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Incorrect germline assignment #69

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dg1002 opened this issue Oct 24, 2023 · 0 comments
Open

Incorrect germline assignment #69

dg1002 opened this issue Oct 24, 2023 · 0 comments

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@dg1002
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dg1002 commented Oct 24, 2023

With an antibody sequence I have, I get different germline assignments from ANARCI and IgBLAST.
IgBLAST assigned germline IGKV7-301 for my sequence while ANARCI gave IGKV3-1101.
At first, I guessed that it's something about the HMM algorithm that makes the result different.
However, if I run IGKV7-301 germline sequence through ANARCI, it's assigned to be IGKV3-1101.

Does anyone have a good idea why this is so?
Thanks in advance.

FYI, IGKV7-3*01 amino acid sequence is

IGKV7-3*01
DIVLTQSPASLAVSPGQRATITCRASESVSFLGINLIHWYQQKPGQPPKLLIYQASNKDTGVPARFSGSGSGTDFTLTINPVEANDTANYYCLQSKNFP
according to the IMGT database.

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