diff --git a/package-lock.json b/package-lock.json
index f50df50692..976a703a94 100644
--- a/package-lock.json
+++ b/package-lock.json
@@ -26284,9 +26284,9 @@
       }
     },
     "node_modules/mysql2": {
-      "version": "3.11.3",
-      "resolved": "https://registry.npmjs.org/mysql2/-/mysql2-3.11.3.tgz",
-      "integrity": "sha512-Qpu2ADfbKzyLdwC/5d4W7+5Yz7yBzCU05YWt5npWzACST37wJsB23wgOSo00qi043urkiRwXtEvJc9UnuLX/MQ==",
+      "version": "3.11.5",
+      "resolved": "https://registry.npmjs.org/mysql2/-/mysql2-3.11.5.tgz",
+      "integrity": "sha512-0XFu8rUmFN9vC0ME36iBvCUObftiMHItrYFhlCRvFWbLgpNqtC4Br/NmZX1HNCszxT0GGy5QtP+k3Q3eCJPaYA==",
       "dev": true,
       "license": "MIT",
       "dependencies": {
@@ -26309,6 +26309,7 @@
       "resolved": "https://registry.npmjs.org/iconv-lite/-/iconv-lite-0.6.3.tgz",
       "integrity": "sha512-4fCk79wshMdzMp2rH06qWrJE4iolqLhCUH+OiuIgU++RB0+94NlDL81atO7GX55uUKueo0txHNtvEyI6D7WdMw==",
       "dev": true,
+      "license": "MIT",
       "dependencies": {
         "safer-buffer": ">= 2.1.2 < 3.0.0"
       },
@@ -37853,7 +37854,7 @@
         "@types/mocha": "7.0.2",
         "@types/node": "18.18.14",
         "@types/semver": "7.5.8",
-        "mysql2": "3.11.3",
+        "mysql2": "3.11.5",
         "nyc": "15.1.0",
         "rimraf": "5.0.10",
         "semver": "7.6.3",
@@ -48554,7 +48555,7 @@
         "@types/mocha": "7.0.2",
         "@types/node": "18.18.14",
         "@types/semver": "7.5.8",
-        "mysql2": "3.11.3",
+        "mysql2": "3.11.5",
         "nyc": "15.1.0",
         "rimraf": "5.0.10",
         "semver": "7.6.3",
@@ -63019,9 +63020,9 @@
       }
     },
     "mysql2": {
-      "version": "3.11.3",
-      "resolved": "https://registry.npmjs.org/mysql2/-/mysql2-3.11.3.tgz",
-      "integrity": "sha512-Qpu2ADfbKzyLdwC/5d4W7+5Yz7yBzCU05YWt5npWzACST37wJsB23wgOSo00qi043urkiRwXtEvJc9UnuLX/MQ==",
+      "version": "3.11.5",
+      "resolved": "https://registry.npmjs.org/mysql2/-/mysql2-3.11.5.tgz",
+      "integrity": "sha512-0XFu8rUmFN9vC0ME36iBvCUObftiMHItrYFhlCRvFWbLgpNqtC4Br/NmZX1HNCszxT0GGy5QtP+k3Q3eCJPaYA==",
       "dev": true,
       "requires": {
         "aws-ssl-profiles": "^1.1.1",
diff --git a/plugins/node/opentelemetry-instrumentation-mysql2/package.json b/plugins/node/opentelemetry-instrumentation-mysql2/package.json
index dcf3806e56..20de228c25 100644
--- a/plugins/node/opentelemetry-instrumentation-mysql2/package.json
+++ b/plugins/node/opentelemetry-instrumentation-mysql2/package.json
@@ -50,7 +50,7 @@
     "@types/mocha": "7.0.2",
     "@types/node": "18.18.14",
     "@types/semver": "7.5.8",
-    "mysql2": "3.11.3",
+    "mysql2": "3.11.5",
     "nyc": "15.1.0",
     "rimraf": "5.0.10",
     "semver": "7.6.3",
diff --git a/plugins/node/opentelemetry-instrumentation-mysql2/src/instrumentation.ts b/plugins/node/opentelemetry-instrumentation-mysql2/src/instrumentation.ts
index 7557cf7a01..8c90080980 100644
--- a/plugins/node/opentelemetry-instrumentation-mysql2/src/instrumentation.ts
+++ b/plugins/node/opentelemetry-instrumentation-mysql2/src/instrumentation.ts
@@ -31,6 +31,7 @@ import type * as mysqlTypes from 'mysql2';
 import { MySQL2InstrumentationConfig } from './types';
 import {
   getConnectionAttributes,
+  getConnectionPrototypeToInstrument,
   getDbStatement,
   getSpanName,
   once,
@@ -56,7 +57,7 @@ export class MySQL2Instrumentation extends InstrumentationBase<MySQL2Instrumenta
         ['>=1.4.2 <4'],
         (moduleExports: any) => {
           const ConnectionPrototype: mysqlTypes.Connection =
-            moduleExports.Connection.prototype;
+            getConnectionPrototypeToInstrument(moduleExports.Connection);
           if (isWrapped(ConnectionPrototype.query)) {
             this._unwrap(ConnectionPrototype, 'query');
           }
diff --git a/plugins/node/opentelemetry-instrumentation-mysql2/src/utils.ts b/plugins/node/opentelemetry-instrumentation-mysql2/src/utils.ts
index 0a045ff45a..bb0fb763d3 100644
--- a/plugins/node/opentelemetry-instrumentation-mysql2/src/utils.ts
+++ b/plugins/node/opentelemetry-instrumentation-mysql2/src/utils.ts
@@ -146,3 +146,22 @@ export const once = (fn: Function) => {
     return fn(...args);
   };
 };
+
+export function getConnectionPrototypeToInstrument(connection: any) {
+  const connectionPrototype = connection.prototype;
+  const basePrototype = Object.getPrototypeOf(connectionPrototype);
+
+  // mysql2@3.11.5 included a refactoring, where most code was moved out of the `Connection` class and into a shared base
+  // so we need to instrument that instead, see https://github.com/sidorares/node-mysql2/pull/3081
+  // This checks if the functions we're instrumenting are there on the base - we cannot use the presence of a base
+  // prototype since EventEmitter is the base for mysql2@<=3.11.4
+  if (
+    typeof basePrototype?.query === 'function' &&
+    typeof basePrototype?.execute === 'function'
+  ) {
+    return basePrototype;
+  }
+
+  // otherwise instrument the connection directly.
+  return connectionPrototype;
+}
diff --git a/plugins/node/opentelemetry-instrumentation-mysql2/test/mysql.test.ts b/plugins/node/opentelemetry-instrumentation-mysql2/test/mysql.test.ts
index a11480418c..ef14d7d982 100644
--- a/plugins/node/opentelemetry-instrumentation-mysql2/test/mysql.test.ts
+++ b/plugins/node/opentelemetry-instrumentation-mysql2/test/mysql.test.ts
@@ -223,10 +223,14 @@ describe('mysql2', () => {
         });
 
         query.on('end', () => {
-          assert.strictEqual(rows, 1);
-          const spans = memoryExporter.getFinishedSpans();
-          assert.strictEqual(spans.length, 1);
-          assertSpan(spans[0], sql);
+          try {
+            assert.strictEqual(rows, 1);
+            const spans = memoryExporter.getFinishedSpans();
+            assert.strictEqual(spans.length, 1);
+            assertSpan(spans[0], sql);
+          } catch (e) {
+            done(e);
+          }
           done();
         });
       });
@@ -340,8 +344,12 @@ describe('mysql2', () => {
           const spans = memoryExporter.getFinishedSpans();
           assert.strictEqual(spans.length, 1);
           getLastQueries(1).then(([query]) => {
-            assert.doesNotMatch(query, /.*traceparent.*/);
-            done();
+            try {
+              assert.doesNotMatch(query, /.*traceparent.*/);
+              done();
+            } catch (e) {
+              done(e);
+            }
           });
         });
       });