diff --git a/plugins/node/opentelemetry-instrumentation-express/src/utils.ts b/plugins/node/opentelemetry-instrumentation-express/src/utils.ts
index fb7446ba76..d888115a0f 100644
--- a/plugins/node/opentelemetry-instrumentation-express/src/utils.ts
+++ b/plugins/node/opentelemetry-instrumentation-express/src/utils.ts
@@ -154,6 +154,10 @@ export const asErrorAndMessage = (
  * @returns The layer path
  */
 export const getLayerPath = (args: unknown[]) => {
+  if (args[0] instanceof RegExp) {
+    return args[0].toString();
+  }
+
   if (typeof args[0] === 'string') {
     return args[0];
   }
diff --git a/plugins/node/opentelemetry-instrumentation-express/test/express.test.ts b/plugins/node/opentelemetry-instrumentation-express/test/express.test.ts
index baac4d40ff..cc194c6244 100644
--- a/plugins/node/opentelemetry-instrumentation-express/test/express.test.ts
+++ b/plugins/node/opentelemetry-instrumentation-express/test/express.test.ts
@@ -567,7 +567,7 @@ describe('ExpressInstrumentation', () => {
     });
   });
 
-  it('should work with ESM usage and multiple routes including regex', async () => {
+  it('should set a correct transaction name for routes specified in RegEx', async () => {
     await testUtils.runTestFixture({
       cwd: __dirname,
       argv: ['fixtures/use-express-regex.mjs'],
@@ -575,6 +575,7 @@ describe('ExpressInstrumentation', () => {
         NODE_OPTIONS:
           '--experimental-loader=@opentelemetry/instrumentation/hook.mjs',
         NODE_NO_WARNINGS: '1',
+        TEST_REGEX_ROUTE: '/test/regex',
       },
       checkResult: (err, stdout, stderr) => {
         assert.ifError(err);
@@ -582,9 +583,7 @@ describe('ExpressInstrumentation', () => {
       checkCollector: (collector: testUtils.TestCollector) => {
         const spans = collector.sortedSpans;
 
-        console.debug(spans);
-
-        assert.strictEqual(spans[0].name, 'GET /test/arr/:id,/\\\/test\\\/arr[0-9]*\\\/required(path)?(\\\/optionalPath)?\\\/(lastParam)?/');
+        assert.strictEqual(spans[0].name, 'GET /\\/test\\/regex/');
         assert.strictEqual(spans[0].kind, SpanKind.CLIENT);
         assert.strictEqual(spans[1].name, 'middleware - query');
         assert.strictEqual(spans[1].kind, SpanKind.SERVER);
@@ -595,10 +594,106 @@ describe('ExpressInstrumentation', () => {
         assert.strictEqual(spans[3].name, 'middleware - simpleMiddleware');
         assert.strictEqual(spans[3].kind, SpanKind.SERVER);
         assert.strictEqual(spans[3].parentSpanId, spans[0].spanId);
-        assert.strictEqual(spans[4].name, 'request handler - /test/arr/:id,/\\\/test\\\/arr[0-9]*\\\/required(path)?(\\\/optionalPath)?\\\/(lastParam)?/');
+        assert.strictEqual(
+          spans[4].name,
+          'request handler - /\\/test\\/regex/'
+        );
         assert.strictEqual(spans[4].kind, SpanKind.SERVER);
         assert.strictEqual(spans[4].parentSpanId, spans[0].spanId);
       },
     });
   });
+
+  for (const segment of ['array1', 'array5']) {
+    it('should set a correct transaction name for routes consisting of arrays of routes', async () => {
+      await testUtils.runTestFixture({
+        cwd: __dirname,
+        argv: ['fixtures/use-express-regex.mjs'],
+        env: {
+          NODE_OPTIONS:
+            '--experimental-loader=@opentelemetry/instrumentation/hook.mjs',
+          NODE_NO_WARNINGS: '1',
+          TEST_REGEX_ROUTE: `/test/${segment}`,
+        },
+        checkResult: err => {
+          assert.ifError(err);
+        },
+        checkCollector: (collector: testUtils.TestCollector) => {
+          const spans = collector.sortedSpans;
+
+          assert.strictEqual(
+            spans[0].name,
+            'GET /test/array1,/\\/test\\/array[2-9]/'
+          );
+          assert.strictEqual(spans[0].kind, SpanKind.CLIENT);
+          assert.strictEqual(spans[1].name, 'middleware - query');
+          assert.strictEqual(spans[1].kind, SpanKind.SERVER);
+          assert.strictEqual(spans[1].parentSpanId, spans[0].spanId);
+          assert.strictEqual(spans[2].name, 'middleware - expressInit');
+          assert.strictEqual(spans[2].kind, SpanKind.SERVER);
+          assert.strictEqual(spans[2].parentSpanId, spans[0].spanId);
+          assert.strictEqual(spans[3].name, 'middleware - simpleMiddleware');
+          assert.strictEqual(spans[3].kind, SpanKind.SERVER);
+          assert.strictEqual(spans[3].parentSpanId, spans[0].spanId);
+          assert.strictEqual(
+            spans[4].name,
+            'request handler - /test/array1,/\\/test\\/array[2-9]/'
+          );
+          assert.strictEqual(spans[4].kind, SpanKind.SERVER);
+          assert.strictEqual(spans[4].parentSpanId, spans[0].spanId);
+        },
+      });
+    });
+  }
+
+  for (const segment of [
+    'arr/545',
+    'arr/required',
+    'arr/required',
+    'arr/requiredPath',
+    'arr/required/lastParam',
+    'arr55/required/lastParam',
+    'arr/requiredPath/optionalPath/',
+    'arr/requiredPath/optionalPath/lastParam',
+  ]) {
+    it('should handle more complex regexes in route arrays correctly', async () => {
+      await testUtils.runTestFixture({
+        cwd: __dirname,
+        argv: ['fixtures/use-express-regex.mjs'],
+        env: {
+          NODE_OPTIONS:
+            '--experimental-loader=@opentelemetry/instrumentation/hook.mjs',
+          NODE_NO_WARNINGS: '1',
+          TEST_REGEX_ROUTE: `/test/${segment}`,
+        },
+        checkResult: err => {
+          assert.ifError(err);
+        },
+        checkCollector: (collector: testUtils.TestCollector) => {
+          const spans = collector.sortedSpans;
+
+          assert.strictEqual(
+            spans[0].name,
+            'GET /test/arr/:id,/\\/test\\/arr[0-9]*\\/required(path)?(\\/optionalPath)?\\/(lastParam)?/'
+          );
+          assert.strictEqual(spans[0].kind, SpanKind.CLIENT);
+          assert.strictEqual(spans[1].name, 'middleware - query');
+          assert.strictEqual(spans[1].kind, SpanKind.SERVER);
+          assert.strictEqual(spans[1].parentSpanId, spans[0].spanId);
+          assert.strictEqual(spans[2].name, 'middleware - expressInit');
+          assert.strictEqual(spans[2].kind, SpanKind.SERVER);
+          assert.strictEqual(spans[2].parentSpanId, spans[0].spanId);
+          assert.strictEqual(spans[3].name, 'middleware - simpleMiddleware');
+          assert.strictEqual(spans[3].kind, SpanKind.SERVER);
+          assert.strictEqual(spans[3].parentSpanId, spans[0].spanId);
+          assert.strictEqual(
+            spans[4].name,
+            'request handler - /test/arr/:id,/\\/test\\/arr[0-9]*\\/required(path)?(\\/optionalPath)?\\/(lastParam)?/'
+          );
+          assert.strictEqual(spans[4].kind, SpanKind.SERVER);
+          assert.strictEqual(spans[4].parentSpanId, spans[0].spanId);
+        },
+      });
+    });
+  }
 });
diff --git a/plugins/node/opentelemetry-instrumentation-express/test/fixtures/use-express-regex.mjs b/plugins/node/opentelemetry-instrumentation-express/test/fixtures/use-express-regex.mjs
index e5aebae064..facba6b16c 100644
--- a/plugins/node/opentelemetry-instrumentation-express/test/fixtures/use-express-regex.mjs
+++ b/plugins/node/opentelemetry-instrumentation-express/test/fixtures/use-express-regex.mjs
@@ -25,11 +25,8 @@ import { ExpressInstrumentation } from '../../build/src/index.js';
 
 const sdk = createTestNodeSdk({
   serviceName: 'use-express-regex',
-  instrumentations: [
-    new HttpInstrumentation(),
-    new ExpressInstrumentation()
-  ]
-})
+  instrumentations: [new HttpInstrumentation(), new ExpressInstrumentation()],
+});
 sdk.start();
 
 import express from 'express';
@@ -46,8 +43,22 @@ app.use(async function simpleMiddleware(req, res, next) {
   next();
 });
 
-app.get(['/test/arr/:id', /\/test\/arr[0-9]*\/required(path)?(\/optionalPath)?\/(lastParam)?/], (_req, res) => {
-  res.send({ response: 'response' });
+app.get(
+  [
+    '/test/arr/:id',
+    /\/test\/arr[0-9]*\/required(path)?(\/optionalPath)?\/(lastParam)?/,
+  ],
+  (_req, res) => {
+    res.send({ response: 'response' });
+  }
+);
+
+app.get(/\/test\/regex/, (_req, res) => {
+  res.send({ response: 'response 2' });
+});
+
+app.get(['/test/array1', /\/test\/array[2-9]/], (_req, res) => {
+  res.send({ response: 'response 3' });
 });
 
 const server = http.createServer(app);
@@ -55,12 +66,15 @@ await new Promise(resolve => server.listen(0, resolve));
 const port = server.address().port;
 
 await new Promise(resolve => {
-  http.get(`http://localhost:${port}/test/arr/requiredPath/optionalPath/lastParam`, (res) => {
-    res.resume();
-    res.on('end', () => {
-      resolve();
-    });
-  })
+  http.get(
+    `http://localhost:${port}${process.env.TEST_REGEX_ROUTE}`,
+    res => {
+      res.resume();
+      res.on('end', () => {
+        resolve();
+      });
+    }
+  );
 });
 
 await new Promise(resolve => server.close(resolve));