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Workaround for iVar not outputting separate reference segments in consensus sequences #25

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nrminor opened this issue Sep 30, 2024 · 0 comments
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bug Something isn't working enhancement New feature or request help wanted Extra attention is needed

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nrminor commented Sep 30, 2024

Ivar, which oneroof uses for variant- and consensus-calling, currently doesn't support outputting multiple reference segments in consensus sequences. This is inconvenient for multi-segment viruses like H5N1 (not to mention for anything with chromosomes). Until the iVar team adds support, it might be worth writing a workaround that separates reads aligned to each segment into its own consensus-calling step and then remerges them afterward.

@nrminor nrminor added bug Something isn't working enhancement New feature or request help wanted Extra attention is needed labels Sep 30, 2024
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