diff --git a/boards/native/nrf_bsim/nrf54l15bsim_nrf54l15_cpuapp.dts b/boards/native/nrf_bsim/nrf54l15bsim_nrf54l15_cpuapp.dts
index 1a38b094795..9a2d3e25c97 100644
--- a/boards/native/nrf_bsim/nrf54l15bsim_nrf54l15_cpuapp.dts
+++ b/boards/native/nrf_bsim/nrf54l15bsim_nrf54l15_cpuapp.dts
@@ -28,7 +28,6 @@
 		/delete-node/ memory@20000000;
 		/delete-node/ memory@2002f000;
 		peripheral@50000000 {
-			/delete-node/ spi@4a000;
 			/delete-node/ vpr@4c000;
 			/delete-node/ mailbox@0;
 			/delete-node/ interrupt-controller@f0000000;
@@ -84,6 +83,10 @@
 	};
 };
 
+&spi00 {
+	status = "disabled";
+};
+
 &uart20 {
 	status = "okay";
 	current-speed = <115200>;