diff --git a/lib/internal/test_runner/runner.js b/lib/internal/test_runner/runner.js
index cc9bcc09b714fe..c4194923cc0f44 100644
--- a/lib/internal/test_runner/runner.js
+++ b/lib/internal/test_runner/runner.js
@@ -318,7 +318,7 @@ class FileTest extends Test {
 
 function runTestFile(path, filesWatcher, opts) {
   const watchMode = filesWatcher != null;
-  const subtest = opts.root.createSubtest(FileTest, path, async (t) => {
+  const subtest = opts.root.createSubtest(FileTest, path, { __proto__: null, signal: opts.signal }, async (t) => {
     const args = getRunArgs(path, opts);
     const stdio = ['pipe', 'pipe', 'pipe'];
     const env = { __proto__: null, ...process.env, NODE_TEST_CONTEXT: 'child-v8' };
diff --git a/test/parallel/test-runner-run.mjs b/test/parallel/test-runner-run.mjs
index 0d7aa346409647..911f115e5fb6a4 100644
--- a/test/parallel/test-runner-run.mjs
+++ b/test/parallel/test-runner-run.mjs
@@ -176,6 +176,17 @@ describe('require(\'node:test\').run', { concurrency: true }, () => {
   });
 
   describe('AbortSignal', () => {
+    it('should accept a signal', async () => {
+      const stream = run({ signal: AbortSignal.timeout(50), files: [
+        fixtures.path('test-runner', 'never_ending_sync.js'),
+        fixtures.path('test-runner', 'never_ending_async.js'),
+      ] });
+      stream.on('test:fail', common.mustCall(2));
+      stream.on('test:pass', common.mustNotCall());
+      // eslint-disable-next-line no-unused-vars
+      for await (const _ of stream);
+    });
+
     it('should stop watch mode when abortSignal aborts', async () => {
       const controller = new AbortController();
       const result = await run({