diff --git a/.zenodo.json b/.zenodo.json
index 4b8ae1f2a6..6e884e7594 100644
--- a/.zenodo.json
+++ b/.zenodo.json
@@ -911,6 +911,11 @@
       "name": "Wu, Jianxiao",
       "orcid": "0000-0002-4866-272X",
     },
+    {
+      "affiliation": "Lund University",
+      "name": "Anijärv, Toomas Erik",
+      "orcid": "0000-0002-3650-4230",
+    },
   ],
   "keywords": [
     "neuroimaging",
diff --git a/nipype/interfaces/mrtrix3/utils.py b/nipype/interfaces/mrtrix3/utils.py
index 6909f380be..3a35e7e534 100644
--- a/nipype/interfaces/mrtrix3/utils.py
+++ b/nipype/interfaces/mrtrix3/utils.py
@@ -11,6 +11,7 @@
     traits,
     TraitedSpec,
     File,
+    Directory,
     InputMultiPath,
     isdefined,
 )
@@ -224,13 +225,16 @@ class Generate5ttInputSpec(MRTrix3BaseInputSpec):
         "fsl",
         "gif",
         "freesurfer",
+        "hsvs",
         argstr="%s",
         position=-3,
         mandatory=True,
         desc="tissue segmentation algorithm",
     )
-    in_file = File(
-        exists=True, argstr="%s", mandatory=True, position=-2, desc="input image"
+    in_file = traits.Either(
+        File(exists=True),
+        Directory(exists=True),
+        argstr="%s", mandatory=True, position=-2, desc="input image / directory"
     )
     out_file = File(argstr="%s", mandatory=True, position=-1, desc="output image")