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Error while parsing gtf #17

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asundaresan1 opened this issue Aug 14, 2024 · 2 comments
Open

Error while parsing gtf #17

asundaresan1 opened this issue Aug 14, 2024 · 2 comments
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bug Something isn't working

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@asundaresan1
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Description of the bug

Hello,

I am getting the follwoing error when i run tf activity pipeline

Command output:
[1] "Reading genes.gtf elements of type gene"
[1] "Found 0 features"

Command error:
[1] "Reading genes.gtf elements of type gene"
[1] "Found 0 features"
gene_id is not a valid field

I am using Rat ensembl GTF.
Another issue is I am not able to use narrowPeak files. I had to rename them to broadPeak in order to process them.

Command used and terminal output

nextflow run nfcore/pipelines/tfactivity/dev/workflow \
            --input samplesheet.csv \
            --outdir ./results \
            -profile  singularity \
            --counts hisat.counts_for_tfactivity.csv \
            --counts_design rnaseq_design.csv \
            -config genome.config \
            --genome rat-ensembl-rn6-r102 \
            --taxon_id 10116

Relevant files

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System information

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@asundaresan1 asundaresan1 added the bug Something isn't working label Aug 14, 2024
@nictru
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nictru commented Aug 18, 2024

Hey, could you provide more detailed error information? E.g. the .nextflow.log file? It's very hard to find out where in the pipeline this error occurred without more information.

@asundaresan1
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error.log
Hi @nictru ,

Attached is the nextflow.log

Thanks!

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