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strandness error #1420

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aheravi opened this issue Oct 17, 2024 · 2 comments
Open

strandness error #1420

aheravi opened this issue Oct 17, 2024 · 2 comments
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@aheravi
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aheravi commented Oct 17, 2024

Description of the bug

Hi there,
Could you comment on my error on strandness below.
My sample sheet is something like this:

sample,fastq_1,fastq_2,strandedness
MaleKO_S1,/projects/S1.R1.fastq.gz,/projects/S1.R2.fastq.gz,auto
...
STQ_QC_TRIM_FILTER_SETSTRANDEDNESS:FASTQ_SUBSAMPLE_FQ_SALMON:SALMON_QUANT (MaleWT_F143557); status: COMPLETED; exit: 0; error: -; workDir: /p
rojects/work/17/bfcfef3c832d1422450c1ec6be7658]
Oct.-17 12:05:01.059 [Actor Thread 113] ERROR nextflow.extension.OperatorImpl - @unknown
groovy.lang.MissingPropertyException: No such property: strandedness for class: Script_6526763402877652
        at org.codehaus.groovy.runtime.ScriptBytecodeAdapter.unwrap(ScriptBytecodeAdapter.java:65)

Command used and terminal output

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@aheravi aheravi added the bug Something isn't working label Oct 17, 2024
@tdanhorn
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Can you confirm that this fails reproducibly with the newest version 3.16.1 that came out yesterday? It would probably also be helpful if you could post the .nextflow.log and any information about your configuration (where you are running it, any config files and profiles, etc.).

@aheravi
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aheravi commented Oct 17, 2024

Acutally it is 3.15.1.
nf-core/rnaseq v3.15.1-g4053b2e
How can I run the latest?

How I run it (on a linux machine):

nextflow-23.10.0-all run nf-core/rnaseq \
    --input /projects/samplesheet_male_ko_wt.csv \
    --outdir /projects/output_male_KO_WT \
    --gtf /projects/hg38_no_alt/annotations/ensembl100/Homo_sapiens.GRCh38.100.remapped.gtf \
    --fasta /projects/hg38_no_alt/genome/fasta/hg38_no_alt.fa \
    -profile singularity \
    --aligner star_rsem \

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