From f5a1c41f76d085d9e5b9a9bfe80ef45ce98393f8 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Tue, 19 Dec 2023 13:34:38 +0100 Subject: [PATCH 01/64] fix vcf_collect when a tool is absent --- CHANGELOG.md | 12 ++++++++++++ assets/multiqc_config.yml | 4 ++-- bin/vcf_collect.py | 5 ++++- nextflow.config | 2 +- 4 files changed, 19 insertions(+), 4 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 5808266d..a4394542 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,6 +3,18 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). +## v3.0.2 - [date] + +### Added + +### Changed + +### Fixed + +- fix VCF_COLLECT handling when a tool is absent from FUSIONREPORT report + +### Removed + ## v3.0.1 - [2023-11-29] ### Added diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index dd83ce70..5930000d 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/rnafusion + This report has been generated by the nf-core/rnafusion analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: nf-core-rnafusion-methods-description: diff --git a/bin/vcf_collect.py b/bin/vcf_collect.py index a8ab85f4..2c25574f 100755 --- a/bin/vcf_collect.py +++ b/bin/vcf_collect.py @@ -346,8 +346,11 @@ def read_build_fusionreport(fusionreport_file: str) -> pd.DataFrame: def read_fusionreport_csv(file: str) -> pd.DataFrame: df = pd.read_csv(file) - df[["starfusion", "arriba", "fusioncatcher"]] = df[["starfusion", "arriba", "fusioncatcher"]].astype("str") columns_to_iterate = ["starfusion", "arriba", "fusioncatcher"] + for column in columns_to_iterate: + if column not in df.columns: + df[column] = "" + df[["starfusion", "arriba", "fusioncatcher"]] = df[["starfusion", "arriba", "fusioncatcher"]].astype("str") for index, row in df.iterrows(): for column in columns_to_iterate: cell_value = row[column] diff --git a/nextflow.config b/nextflow.config index 3a54be0c..b25aa1e3 100644 --- a/nextflow.config +++ b/nextflow.config @@ -290,7 +290,7 @@ manifest { description = """Nextflow rnafusion analysis pipeline, part of the nf-core community.""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '3.0.1' + version = '3.0.2dev' doi = '' } From 266f007e4cd26c25ac4334aea83f8163f94fb10a Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 19 Dec 2023 19:46:36 +0000 Subject: [PATCH 02/64] Template update for nf-core/tools version 2.11 --- .github/CONTRIBUTING.md | 3 + .github/PULL_REQUEST_TEMPLATE.md | 1 + .github/workflows/ci.yml | 2 +- .github/workflows/fix-linting.yml | 4 +- .github/workflows/linting.yml | 12 +- .gitpod.yml | 4 +- CHANGELOG.md | 2 +- README.md | 17 +-- assets/multiqc_config.yml | 2 +- assets/slackreport.json | 2 +- conf/modules.config | 2 +- docs/usage.md | 4 +- lib/NfcoreTemplate.groovy | 32 ++--- modules.json | 6 +- .../dumpsoftwareversions/environment.yml | 7 ++ .../custom/dumpsoftwareversions/main.nf | 6 +- .../custom/dumpsoftwareversions/meta.yml | 7 +- .../dumpsoftwareversions/tests/main.nf.test | 38 ++++++ .../tests/main.nf.test.snap | 27 +++++ .../dumpsoftwareversions/tests/tags.yml | 2 + modules/nf-core/fastqc/environment.yml | 7 ++ modules/nf-core/fastqc/main.nf | 10 +- modules/nf-core/fastqc/meta.yml | 5 + modules/nf-core/fastqc/tests/main.nf.test | 109 ++++++++++++++++++ .../nf-core/fastqc/tests/main.nf.test.snap | 10 ++ modules/nf-core/fastqc/tests/tags.yml | 2 + modules/nf-core/multiqc/environment.yml | 7 ++ modules/nf-core/multiqc/main.nf | 8 +- modules/nf-core/multiqc/meta.yml | 11 +- modules/nf-core/multiqc/tests/main.nf.test | 63 ++++++++++ modules/nf-core/multiqc/tests/tags.yml | 2 + nextflow.config | 12 +- 32 files changed, 360 insertions(+), 66 deletions(-) create mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml create mode 100644 modules/nf-core/fastqc/environment.yml create mode 100644 modules/nf-core/fastqc/tests/main.nf.test create mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap create mode 100644 modules/nf-core/fastqc/tests/tags.yml create mode 100644 modules/nf-core/multiqc/environment.yml create mode 100644 modules/nf-core/multiqc/tests/main.nf.test create mode 100644 modules/nf-core/multiqc/tests/tags.yml diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 5e32dfd8..a0b73d5c 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -27,6 +27,9 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests +You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to +receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. + When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 5cc076f3..ed1d027b 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -19,6 +19,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnaf - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. - [ ] `CHANGELOG.md` is updated. diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index a6726dd9..31823f13 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,7 +28,7 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 14c6c08a..4b02fe04 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -13,7 +13,7 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 with: token: ${{ secrets.nf_core_bot_auth_token }} @@ -24,7 +24,7 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install Prettier run: npm install -g prettier @prettier/plugin-php diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index b8bdd214..905c58e4 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,9 +14,9 @@ jobs: EditorConfig: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install editorconfig-checker run: npm install -g editorconfig-checker @@ -27,9 +27,9 @@ jobs: Prettier: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install Prettier run: npm install -g prettier @@ -40,7 +40,7 @@ jobs: PythonBlack: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - name: Check code lints with Black uses: psf/black@stable @@ -71,7 +71,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 diff --git a/.gitpod.yml b/.gitpod.yml index 25488dcc..acf72695 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -4,7 +4,9 @@ tasks: command: | pre-commit install --install-hooks nextflow self-update - + - name: unset JAVA_TOOL_OPTIONS + command: | + unset JAVA_TOOL_OPTIONS vscode: extensions: # based on nf-core.nf-core-extensionpack - codezombiech.gitignore # Language support for .gitignore files diff --git a/CHANGELOG.md b/CHANGELOG.md index d09f1be9..a01e6117 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v3.0.0dev - [date] +## v3.0.2dev - [date] Initial release of nf-core/rnafusion, created with the [nf-core](https://nf-co.re/) template. diff --git a/README.md b/README.md index b4605ebd..d79c9677 100644 --- a/README.md +++ b/README.md @@ -30,11 +30,8 @@ ## Usage -:::note -If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how -to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) -with `-profile test` before running the workflow on actual data. -::: +> [!NOTE] +> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. - + diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 918aa9d3..b8874305 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,5 +1,5 @@ report_comment: > - This report has been generated by the nf-core/rnafusion + This report has been generated by the nf-core/rnafusion analysis pipeline. For information about how to interpret these results, please see the documentation. report_section_order: diff --git a/assets/slackreport.json b/assets/slackreport.json index 1b3cba01..66b6e474 100644 --- a/assets/slackreport.json +++ b/assets/slackreport.json @@ -3,7 +3,7 @@ { "fallback": "Plain-text summary of the attachment.", "color": "<% if (success) { %>good<% } else { %>danger<%} %>", - "author_name": "nf-core/rnafusion v${version} - ${runName}", + "author_name": "nf-core/rnafusion ${version} - ${runName}", "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", "fields": [ diff --git a/conf/modules.config b/conf/modules.config index 39e81386..d91c6aba 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -39,7 +39,7 @@ process { } withName: 'MULTIQC' { - ext.args = params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' + ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ path: { "${params.outdir}/multiqc" }, mode: params.publish_dir_mode, diff --git a/docs/usage.md b/docs/usage.md index 4c90522f..a39d7dc4 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -20,7 +20,7 @@ You will need to create a samplesheet with information about the samples you wou The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: -```console +```csv title="samplesheet.csv" sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz @@ -33,7 +33,7 @@ The pipeline will auto-detect whether a sample is single- or paired-end using th A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. -```console +```csv title="samplesheet.csv" sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy index 01b8653d..e248e4c3 100755 --- a/lib/NfcoreTemplate.groovy +++ b/lib/NfcoreTemplate.groovy @@ -4,6 +4,7 @@ import org.yaml.snakeyaml.Yaml import groovy.json.JsonOutput +import nextflow.extension.FilesEx class NfcoreTemplate { @@ -141,12 +142,14 @@ class NfcoreTemplate { try { if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } [ 'sendmail', '-t' ].execute() << sendmail_html log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" } catch (all) { // Catch failures and try with plaintext def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { mail_cmd += [ '-A', mqc_report ] } mail_cmd.execute() << email_html @@ -155,14 +158,16 @@ class NfcoreTemplate { } // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") + FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() } // @@ -227,15 +232,14 @@ class NfcoreTemplate { // Dump pipeline parameters in a json file // public static void dump_parameters(workflow, params) { - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def output_pf = new File(output_d, "params_${timestamp}.json") + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") def jsonStr = JsonOutput.toJson(params) - output_pf.text = JsonOutput.prettyPrint(jsonStr) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() } // diff --git a/modules.json b/modules.json index 91f35b97..ea036a48 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "bd8092b67b5103bdd52e300f75889442275c3117", + "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "4ab13872435962dadc239979554d13709e20bf29", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml new file mode 100644 index 00000000..f0c63f69 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -0,0 +1,7 @@ +name: custom_dumpsoftwareversions +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.17 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index ebc87273..7685b33c 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : + 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml index c32657de..5f15a5fd 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml @@ -1,4 +1,4 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: custom_dumpsoftwareversions description: Custom module used to dump software versions within the nf-core pipeline template keywords: @@ -16,7 +16,6 @@ input: type: file description: YML file containing software versions pattern: "*.yml" - output: - yml: type: file @@ -30,7 +29,9 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@drpatelh" - "@grst" +maintainers: + - "@drpatelh" + - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test new file mode 100644 index 00000000..eec1db10 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -0,0 +1,38 @@ +nextflow_process { + + name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" + script "../main.nf" + process "CUSTOM_DUMPSOFTWAREVERSIONS" + tag "modules" + tag "modules_nfcore" + tag "custom" + tag "dumpsoftwareversions" + tag "custom/dumpsoftwareversions" + + test("Should run without failures") { + when { + process { + """ + def tool1_version = ''' + TOOL1: + tool1: 0.11.9 + '''.stripIndent() + + def tool2_version = ''' + TOOL2: + tool2: 1.9 + '''.stripIndent() + + input[0] = Channel.of(tool1_version, tool2_version).collectFile() + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap new file mode 100644 index 00000000..4274ed57 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -0,0 +1,27 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ], + "1": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "2": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "mqc_yml": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "versions": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "yml": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ] + } + ], + "timestamp": "2023-11-03T14:43:22.157011" + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml new file mode 100644 index 00000000..405aa24a --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml @@ -0,0 +1,2 @@ +custom/dumpsoftwareversions: + - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml new file mode 100644 index 00000000..1787b38a --- /dev/null +++ b/modules/nf-core/fastqc/environment.yml @@ -0,0 +1,7 @@ +name: fastqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 249f9064..9e19a74c 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -2,10 +2,10 @@ process FASTQC { tag "$meta.id" label 'process_medium' - conda "bioconda::fastqc=0.11.9" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' : - 'biocontainers/fastqc:0.11.9--0' }" + 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : + 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" input: tuple val(meta), path(reads) @@ -37,7 +37,7 @@ process FASTQC { cat <<-END_VERSIONS > versions.yml "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) END_VERSIONS """ @@ -49,7 +49,7 @@ process FASTQC { cat <<-END_VERSIONS > versions.yml "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) END_VERSIONS """ } diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml index 4da5bb5a..ee5507e0 100644 --- a/modules/nf-core/fastqc/meta.yml +++ b/modules/nf-core/fastqc/meta.yml @@ -50,3 +50,8 @@ authors: - "@grst" - "@ewels" - "@FelixKrueger" +maintainers: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test new file mode 100644 index 00000000..b9e8f926 --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -0,0 +1,109 @@ +nextflow_process { + + name "Test Process FASTQC" + script "../main.nf" + process "FASTQC" + tag "modules" + tag "modules_nfcore" + tag "fastqc" + + test("Single-Read") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id: 'test', single_end:true ], + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. + // looks like this:
Mon 2 Oct 2023
test.gz
+ // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 + { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, + { assert path(process.out.html.get(0).get(1)).getText().contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match("versions") }, + { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + ) + } + } +// TODO +// // +// // Test with paired-end data +// // +// workflow test_fastqc_paired_end { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with interleaved data +// // +// workflow test_fastqc_interleaved { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with bam data +// // +// workflow test_fastqc_bam { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with multiple samples +// // +// workflow test_fastqc_multiple { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with custom prefix +// // +// workflow test_fastqc_custom_prefix { +// input = [ +// [ id:'mysample', single_end:true ], // meta map +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } +} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap new file mode 100644 index 00000000..636a32ce --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -0,0 +1,10 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2023-10-09T23:40:54+0000" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml new file mode 100644 index 00000000..7834294b --- /dev/null +++ b/modules/nf-core/fastqc/tests/tags.yml @@ -0,0 +1,2 @@ +fastqc: + - modules/nf-core/fastqc/** diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml new file mode 100644 index 00000000..bc0bdb5b --- /dev/null +++ b/modules/nf-core/multiqc/environment.yml @@ -0,0 +1,7 @@ +name: multiqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.18 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1fc387be..00cc48d2 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -1,10 +1,10 @@ process MULTIQC { label 'process_single' - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : + 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -25,12 +25,14 @@ process MULTIQC { def args = task.ext.args ?: '' def config = multiqc_config ? "--config $multiqc_config" : '' def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' + def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' """ multiqc \\ --force \\ $args \\ $config \\ $extra_config \\ + $logo \\ . cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f93b5ee5..f1aa660e 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,5 +1,5 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json -name: MultiQC +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: - QC @@ -13,7 +13,6 @@ tools: homepage: https://multiqc.info/ documentation: https://multiqc.info/docs/ licence: ["GPL-3.0-or-later"] - input: - multiqc_files: type: file @@ -31,7 +30,6 @@ input: type: file description: Optional logo file for MultiQC pattern: "*.{png}" - output: - report: type: file @@ -54,3 +52,8 @@ authors: - "@bunop" - "@drpatelh" - "@jfy133" +maintainers: + - "@abhi18av" + - "@bunop" + - "@drpatelh" + - "@jfy133" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test new file mode 100644 index 00000000..c2dad217 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -0,0 +1,63 @@ +nextflow_process { + + name "Test Process MULTIQC" + script "../main.nf" + process "MULTIQC" + tag "modules" + tag "modules_nfcore" + tag "multiqc" + + test("MULTIQC: FASTQC") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.report.get(0)).exists() }, + { assert path(process.out.data.get(0)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + ) + } + + } + + test("MULTIQC: FASTQC and a config file") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.report.get(0)).exists() }, + { assert path(process.out.data.get(0)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + ) + } + + } +} diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml new file mode 100644 index 00000000..bea6c0d3 --- /dev/null +++ b/modules/nf-core/multiqc/tests/tags.yml @@ -0,0 +1,2 @@ +multiqc: + - modules/nf-core/multiqc/** diff --git a/nextflow.config b/nextflow.config index 6d83e938..3d62ebf6 100644 --- a/nextflow.config +++ b/nextflow.config @@ -14,7 +14,7 @@ params { input = null // References genome = null - igenomes_base = 's3://ngi-igenomes/igenomes' + igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false @@ -82,6 +82,7 @@ profiles { dumpHashes = true process.beforeScript = 'echo $HOSTNAME' cleanup = false + nextflow.enable.configProcessNamesValidation = true } conda { conda.enabled = true @@ -104,13 +105,13 @@ profiles { } docker { docker.enabled = true - docker.userEmulation = true conda.enabled = false singularity.enabled = false podman.enabled = false shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false + runOptions = '-u $(id -u):$(id -g)' } arm { docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' @@ -181,7 +182,7 @@ singularity.registry = 'quay.io' // Nextflow plugins plugins { - id 'nf-validation' // Validation of pipeline parameters and creation of an input channel from a sample sheet + id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet } // Load igenomes.config if required @@ -204,6 +205,9 @@ env { // Capture exit codes from upstream processes when piping process.shell = ['/bin/bash', '-euo', 'pipefail'] +// Disable process selector warnings by default. Use debug profile to enable warnings. +nextflow.enable.configProcessNamesValidation = false + def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { enabled = true @@ -229,7 +233,7 @@ manifest { description = """Nextflow rnafusion analysis pipeline, part of the nf-core community.""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '3.0.0dev' + version = '3.0.2dev' doi = '' } From cfb0833cc5cb21537b8885d1d8f06c57ebc02e32 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Wed, 20 Dec 2023 15:57:49 +0000 Subject: [PATCH 03/64] Template update for nf-core/tools version 2.11.1 --- .../{release-announcments.yml => release-announcements.yml} | 0 nextflow.config | 4 ++-- 2 files changed, 2 insertions(+), 2 deletions(-) rename .github/workflows/{release-announcments.yml => release-announcements.yml} (100%) diff --git a/.github/workflows/release-announcments.yml b/.github/workflows/release-announcements.yml similarity index 100% rename from .github/workflows/release-announcments.yml rename to .github/workflows/release-announcements.yml diff --git a/nextflow.config b/nextflow.config index 3d62ebf6..9e5ac5da 100644 --- a/nextflow.config +++ b/nextflow.config @@ -111,10 +111,10 @@ profiles { shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false - runOptions = '-u $(id -u):$(id -g)' + docker.runOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { singularity.enabled = true From 0ccea2e1c1f4e593979ac7e1bdf3c10dd73b1c21 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Wed, 20 Dec 2023 17:38:10 +0100 Subject: [PATCH 04/64] Revert "fix vcf_collect when a tool is absent" This reverts commit f5a1c41f76d085d9e5b9a9bfe80ef45ce98393f8. --- CHANGELOG.md | 12 ------------ assets/multiqc_config.yml | 4 ++-- bin/vcf_collect.py | 5 +---- nextflow.config | 2 +- 4 files changed, 4 insertions(+), 19 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index a4394542..5808266d 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,18 +3,6 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v3.0.2 - [date] - -### Added - -### Changed - -### Fixed - -- fix VCF_COLLECT handling when a tool is absent from FUSIONREPORT report - -### Removed - ## v3.0.1 - [2023-11-29] ### Added diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 5930000d..dd83ce70 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/rnafusion + This report has been generated by the nf-core/rnafusion analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: nf-core-rnafusion-methods-description: diff --git a/bin/vcf_collect.py b/bin/vcf_collect.py index 2c25574f..a8ab85f4 100755 --- a/bin/vcf_collect.py +++ b/bin/vcf_collect.py @@ -346,11 +346,8 @@ def read_build_fusionreport(fusionreport_file: str) -> pd.DataFrame: def read_fusionreport_csv(file: str) -> pd.DataFrame: df = pd.read_csv(file) - columns_to_iterate = ["starfusion", "arriba", "fusioncatcher"] - for column in columns_to_iterate: - if column not in df.columns: - df[column] = "" df[["starfusion", "arriba", "fusioncatcher"]] = df[["starfusion", "arriba", "fusioncatcher"]].astype("str") + columns_to_iterate = ["starfusion", "arriba", "fusioncatcher"] for index, row in df.iterrows(): for column in columns_to_iterate: cell_value = row[column] diff --git a/nextflow.config b/nextflow.config index b25aa1e3..3a54be0c 100644 --- a/nextflow.config +++ b/nextflow.config @@ -290,7 +290,7 @@ manifest { description = """Nextflow rnafusion analysis pipeline, part of the nf-core community.""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '3.0.2dev' + version = '3.0.1' doi = '' } From d76dfc49441415f34f8142ba0bcc4a614ef9ab17 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Wed, 20 Dec 2023 17:43:03 +0100 Subject: [PATCH 05/64] fix vcf_collect when a tool is absent --- CHANGELOG.md | 12 ++++++++++++ assets/multiqc_config.yml | 4 ++-- bin/vcf_collect.py | 5 ++++- nextflow.config | 2 +- 4 files changed, 19 insertions(+), 4 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 5808266d..ef0b02d9 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,6 +3,18 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). +## v3.0.2dev - [date] + +### Added + +### Changed + +### Fixed + +- fix VCF_COLLECT handling when a tool is absent from FUSIONREPORT report + +### Removed + ## v3.0.1 - [2023-11-29] ### Added diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index dd83ce70..5930000d 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/rnafusion + This report has been generated by the nf-core/rnafusion analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: nf-core-rnafusion-methods-description: diff --git a/bin/vcf_collect.py b/bin/vcf_collect.py index a8ab85f4..2c25574f 100755 --- a/bin/vcf_collect.py +++ b/bin/vcf_collect.py @@ -346,8 +346,11 @@ def read_build_fusionreport(fusionreport_file: str) -> pd.DataFrame: def read_fusionreport_csv(file: str) -> pd.DataFrame: df = pd.read_csv(file) - df[["starfusion", "arriba", "fusioncatcher"]] = df[["starfusion", "arriba", "fusioncatcher"]].astype("str") columns_to_iterate = ["starfusion", "arriba", "fusioncatcher"] + for column in columns_to_iterate: + if column not in df.columns: + df[column] = "" + df[["starfusion", "arriba", "fusioncatcher"]] = df[["starfusion", "arriba", "fusioncatcher"]].astype("str") for index, row in df.iterrows(): for column in columns_to_iterate: cell_value = row[column] diff --git a/nextflow.config b/nextflow.config index 3a54be0c..b25aa1e3 100644 --- a/nextflow.config +++ b/nextflow.config @@ -290,7 +290,7 @@ manifest { description = """Nextflow rnafusion analysis pipeline, part of the nf-core community.""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '3.0.1' + version = '3.0.2dev' doi = '' } From d163a4aa8a75fa061c627d54c801e64ee31bf9d7 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Wed, 20 Dec 2023 17:55:55 +0100 Subject: [PATCH 06/64] fix version issue --- assets/multiqc_config.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 4d6c99bc..a50a5992 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > This report has been generated by the nf-core/rnafusion analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: nf-core-rnafusion-methods-description: From 81ca0c0bc0e4e453a04873455b55ebac8cfcc35e Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 29 Jan 2024 14:58:01 +0100 Subject: [PATCH 07/64] prettier --- .devcontainer/devcontainer.json | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4ecfbfe3..4a9bc5c7 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -18,11 +18,11 @@ "python.linting.flake8Path": "/opt/conda/bin/flake8", "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" + "python.linting.pylintPath": "/opt/conda/bin/pylint", }, // Add the IDs of extensions you want installed when the container is created. - "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] - } - } + "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"], + }, + }, } From d0f345a97653c3adae16558ca56d41ddba9cdb00 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 29 Jan 2024 15:13:20 +0000 Subject: [PATCH 08/64] Template update for nf-core/tools version 2.12 --- .editorconfig | 8 + .github/workflows/awsfulltest.yml | 2 +- .github/workflows/awstest.yml | 2 +- .github/workflows/branch.yml | 2 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 67 +++++ .github/workflows/fix-linting.yml | 72 +++-- .github/workflows/linting.yml | 61 +--- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 4 +- .gitpod.yml | 1 + .pre-commit-config.yaml | 7 +- README.md | 8 +- assets/email_template.html | 2 +- assets/email_template.txt | 2 +- assets/nf-core-rnafusion_logo_light.png | Bin 72629 -> 75831 bytes docs/images/nf-core-rnafusion_logo_dark.png | Bin 72761 -> 27749 bytes docs/images/nf-core-rnafusion_logo_light.png | Bin 72629 -> 23933 bytes lib/WorkflowMain.groovy | 16 +- lib/nfcore_external_java_deps.jar | Bin 2291171 -> 0 bytes main.nf | 2 +- modules.json | 6 +- .../dumpsoftwareversions/environment.yml | 2 +- .../custom/dumpsoftwareversions/main.nf | 4 +- .../dumpsoftwareversions/tests/main.nf.test | 7 +- .../tests/main.nf.test.snap | 50 ++-- modules/nf-core/fastqc/tests/main.nf.test | 265 ++++++++++++------ .../nf-core/fastqc/tests/main.nf.test.snap | 12 +- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 6 +- modules/nf-core/multiqc/meta.yml | 1 - modules/nf-core/multiqc/tests/main.nf.test | 48 +++- .../nf-core/multiqc/tests/main.nf.test.snap | 21 ++ nextflow.config | 3 +- pyproject.toml | 17 +- workflows/rnafusion.nf | 7 + 36 files changed, 490 insertions(+), 221 deletions(-) create mode 100644 .github/workflows/download_pipeline.yml delete mode 100644 lib/nfcore_external_java_deps.jar create mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap diff --git a/.editorconfig b/.editorconfig index b6b31907..9b990088 100644 --- a/.editorconfig +++ b/.editorconfig @@ -22,3 +22,11 @@ indent_size = unset [/assets/email*] indent_size = unset + +# ignore Readme +[README.md] +indent_style = unset + +# ignore python +[*.{py}] +indent_style = unset diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 2db9edfa..a4b6b4d3 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@v3 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 5ef7e96c..cf29b8fd 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@v3 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 3867817a..476d72a8 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v1 + uses: mshick/add-pr-comment@v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index 694e90ec..e37cfda5 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v7 + - uses: actions/stale@v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml new file mode 100644 index 00000000..8611458a --- /dev/null +++ b/.github/workflows/download_pipeline.yml @@ -0,0 +1,67 @@ +name: Test successful pipeline download with 'nf-core download' + +# Run the workflow when: +# - dispatched manually +# - when a PR is opened or reopened to master branch +# - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. +on: + workflow_dispatch: + pull_request: + types: + - opened + branches: + - master + pull_request_target: + branches: + - master + +env: + NXF_ANSI_LOG: false + +jobs: + download: + runs-on: ubuntu-latest + steps: + - name: Install Nextflow + uses: nf-core/setup-nextflow@v1 + + - uses: actions/setup-python@v5 + with: + python-version: "3.11" + architecture: "x64" + - uses: eWaterCycle/setup-singularity@v7 + with: + singularity-version: 3.8.3 + + - name: Install dependencies + run: | + python -m pip install --upgrade pip + pip install git+https://github.com/nf-core/tools.git@dev + + - name: Get the repository name and current branch set as environment variable + run: | + echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} + echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + + - name: Download the pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + run: | + nf-core download ${{ env.REPO_LOWERCASE }} \ + --revision ${{ env.REPO_BRANCH }} \ + --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ + --compress "none" \ + --container-system 'singularity' \ + --container-library "quay.io" -l "docker.io" -l "ghcr.io" \ + --container-cache-utilisation 'amend' \ + --download-configuration + + - name: Inspect download + run: tree ./${{ env.REPOTITLE_LOWERCASE }} + + - name: Run the downloaded pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 4b02fe04..36ecd207 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -4,7 +4,7 @@ on: types: [created] jobs: - deploy: + fix-linting: # Only run if comment is on a PR with the main repo, and if it contains the magic keywords if: > contains(github.event.comment.html_url, '/pull/') && @@ -13,10 +13,17 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 with: token: ${{ secrets.nf_core_bot_auth_token }} + # indication that the linting is being fixed + - name: React on comment + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: eyes + # Action runs on the issue comment, so we don't get the PR by default # Use the gh cli to check out the PR - name: Checkout Pull Request @@ -24,32 +31,59 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v4 + # Install and run pre-commit + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + with: + python-version: 3.11 - - name: Install Prettier - run: npm install -g prettier @prettier/plugin-php + - name: Install pre-commit + run: pip install pre-commit - # Check that we actually need to fix something - - name: Run 'prettier --check' - id: prettier_status - run: | - if prettier --check ${GITHUB_WORKSPACE}; then - echo "result=pass" >> $GITHUB_OUTPUT - else - echo "result=fail" >> $GITHUB_OUTPUT - fi + - name: Run pre-commit + id: pre-commit + run: pre-commit run --all-files + continue-on-error: true - - name: Run 'prettier --write' - if: steps.prettier_status.outputs.result == 'fail' - run: prettier --write ${GITHUB_WORKSPACE} + # indication that the linting has finished + - name: react if linting finished succesfully + if: steps.pre-commit.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: "+1" - name: Commit & push changes - if: steps.prettier_status.outputs.result == 'fail' + id: commit-and-push + if: steps.pre-commit.outcome == 'failure' run: | git config user.email "core@nf-co.re" git config user.name "nf-core-bot" git config push.default upstream git add . git status - git commit -m "[automated] Fix linting with Prettier" + git commit -m "[automated] Fix code linting" git push + + - name: react if linting errors were fixed + id: react-if-fixed + if: steps.commit-and-push.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: hooray + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: confused + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + issue-number: ${{ github.event.issue.number }} + body: | + @${{ github.actor }} I tried to fix the linting errors, but it didn't work. Please fix them manually. + See [CI log](https://github.com/nf-core/rnafusion/actions/runs/${{ github.run_id }}) for more details. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 905c58e4..81cd098e 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,61 +11,22 @@ on: types: [published] jobs: - EditorConfig: + pre-commit: runs-on: ubuntu-latest steps: - uses: actions/checkout@v4 - - uses: actions/setup-node@v4 - - - name: Install editorconfig-checker - run: npm install -g editorconfig-checker - - - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') - - Prettier: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - uses: actions/setup-node@v4 - - - name: Install Prettier - run: npm install -g prettier - - - name: Run Prettier --check - run: prettier --check ${GITHUB_WORKSPACE} - - PythonBlack: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - name: Check code lints with Black - uses: psf/black@stable - - # If the above check failed, post a comment on the PR explaining the failure - - name: Post PR comment - if: failure() - uses: mshick/add-pr-comment@v1 + - name: Set up Python 3.11 + uses: actions/setup-python@v5 with: - message: | - ## Python linting (`black`) is failing - - To keep the code consistent with lots of contributors, we run automated code consistency checks. - To fix this CI test, please run: - - * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` - * Fix formatting errors in your pipeline: `black .` - - Once you push these changes the test should pass, and you can hide this comment :+1: + python-version: 3.11 + cache: "pip" - We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + - name: Install pre-commit + run: pip install pre-commit - Thanks again for your contribution! - repo-token: ${{ secrets.GITHUB_TOKEN }} - allow-repeats: false + - name: Run pre-commit + run: pre-commit run --all-files nf-core: runs-on: ubuntu-latest @@ -76,7 +37,7 @@ jobs: - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.11" architecture: "x64" @@ -99,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v3 + uses: actions/upload-artifact@v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 0bbcd30f..147bcd10 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v2 + uses: dawidd6/action-download-artifact@v3 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 6ad33927..21ac3f06 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -24,7 +24,7 @@ jobs: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +56,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.0.2 + - uses: zentered/bluesky-post-action@v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/.gitpod.yml b/.gitpod.yml index acf72695..363d5b1d 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -7,6 +7,7 @@ tasks: - name: unset JAVA_TOOL_OPTIONS command: | unset JAVA_TOOL_OPTIONS + vscode: extensions: # based on nf-core.nf-core-extensionpack - codezombiech.gitignore # Language support for .gitignore files diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 0c31cdb9..af57081f 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -1,5 +1,10 @@ repos: - repo: https://github.com/pre-commit/mirrors-prettier - rev: "v2.7.1" + rev: "v3.1.0" hooks: - id: prettier + - repo: https://github.com/editorconfig-checker/editorconfig-checker.python + rev: "2.7.3" + hooks: + - id: editorconfig-checker + alias: ec diff --git a/README.md b/README.md index d79c9677..c48b6f3b 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,9 @@ -# ![nf-core/rnafusion](docs/images/nf-core-rnafusion_logo_light.png#gh-light-mode-only) ![nf-core/rnafusion](docs/images/nf-core-rnafusion_logo_dark.png#gh-dark-mode-only) - +

+ + + nf-core/rnafusion + +

[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+CI%22) [![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) diff --git a/assets/email_template.html b/assets/email_template.html index efcd0eaa..72ed98c8 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -12,7 +12,7 @@ -

nf-core/rnafusion v${version}

+

nf-core/rnafusion ${version}

Run Name: $runName

<% if (!success){ diff --git a/assets/email_template.txt b/assets/email_template.txt index e1cabd80..bee92f36 100644 --- a/assets/email_template.txt +++ b/assets/email_template.txt @@ -4,7 +4,7 @@ |\\ | |__ __ / ` / \\ |__) |__ } { | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, `._,._,' - nf-core/rnafusion v${version} + nf-core/rnafusion ${version} ---------------------------------------------------- Run Name: $runName diff --git a/assets/nf-core-rnafusion_logo_light.png b/assets/nf-core-rnafusion_logo_light.png index 5609a199d523605d536b8c5bb0aae654af1c4a57..fce712782716cb146bd3b4147dc9821244151318 100644 GIT binary patch literal 75831 zcmeEui93|*|NktQ%2p{POG2_GTh>fbF$vKkWvL`7S+eglWvLVyq^!vx5wgoR)sd*| zWoJr4wy}@>_kPs*ocb4j*Y~ijuVhOY*KysvQ|q$y*J-S zk72oMpYe0q?VAbaq#*9EMu$IdcoXp^so>_-$xz2=@*S~&`he~jv*V1qmOg7gB~|ku z4iN>q9{kdyZ|YpwtFN7@xuSZ(Q#H%X?NoYKwa=aXj}v2~+N64J89ds7jyopzTE&lL zTX=gIP6(U()S#j$694J$cS-q-%;cBHV%V6wo@O1Lq5Km$NZ4lj<2c zwqe?icigw3j2(aYEkjNIUZPcw=4bC$%q+7b?p1P^!$01``3%CuY^87 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zR(SliZInVGdFiX-+?ulq^Pr062u)ng5j(}CTiGLQkv6?rkcPd!;?fH5U^+iRKzvfu zV~;6qm0a{ko%Q&bhwCxwcNjHtrRuX~uPJ4SI{~}WvHI=pn{11L;PnA=|26zUHCTRb zgzHKw^0*vqpCo`j;lJKoyKx8d7!8AI%m}wVX-c@2B_KnHw}>4U#aDXTOq7<|Wd4Tsc3c@~MlU!Do- z;44!I44#TL{egGhbo--w?_)Cnawj*O*6B(*W)rTAlB^EKDv_8PPE_4zdul@}vk=<2 zWM<}+1wca(EgDR~^?BHoZ|m^g5Plb!5RP%HK2`I`LZtE_&_TmfZv$SS=39Ykn@g)h zK2xOBBV8(O>$B1^yv5v)z{*v8xsq2EHw6IY)|ObEUM$)D;2ldBzXL^o2#D*TR7q2+ zDkW3pWRJ7P86=mB!#~|iPx<77n)$##FJ%f5Y(taKVql$zJJ-sa2Lq?0v^>t=^){tS z15cnLfeqNGIDqY|K4E1|Nz)c>_jNb-`iKXYX>tUy3D1%=D4xIVLh5@N-8J$JWP1}C zk1ce0UGgFBPREZ`^YsLWgRZcTtn1;*uP{d&`VCLk^-43Sr>Q}8Ke)}JYvPzo( diff --git a/main.nf b/main.nf index 5003e1b9..47c38e5e 100644 --- a/main.nf +++ b/main.nf @@ -44,7 +44,7 @@ if (params.validate_params) { validateParameters() } -WorkflowMain.initialise(workflow, params, log) +WorkflowMain.initialise(workflow, params, log, args) /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ diff --git a/modules.json b/modules.json index ea036a48..a7097920 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", + "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "4ab13872435962dadc239979554d13709e20bf29", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml index f0c63f69..9b3272bc 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.17 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 7685b33c..f2187611 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -4,8 +4,8 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : - 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test index eec1db10..b1e1630b 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -31,7 +31,12 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot( + process.out.versions, + file(process.out.mqc_yml[0]).readLines()[0..10], + file(process.out.yml[0]).readLines()[0..7] + ).match() + } ) } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap index 4274ed57..5f59a936 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -1,27 +1,33 @@ { "Should run without failures": { "content": [ - { - "0": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ], - "1": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "2": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "mqc_yml": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "versions": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "yml": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ] - } + [ + "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" + ], + [ + "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", + " \\n\\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n \\n \\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n\\n\\n \\n\\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\" + ], + [ + "CUSTOM_DUMPSOFTWAREVERSIONS:", + " python: 3.11.7", + " yaml: 5.4.1", + "TOOL1:", + " tool1: 0.11.9", + "TOOL2:", + " tool2: '1.9'", + "Workflow:" + ] ], - "timestamp": "2023-11-03T14:43:22.157011" + "timestamp": "2024-01-09T23:01:18.710682" } -} +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index b9e8f926..1f21c664 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -3,24 +3,20 @@ nextflow_process { name "Test Process FASTQC" script "../main.nf" process "FASTQC" + tag "modules" tag "modules_nfcore" tag "fastqc" - test("Single-Read") { + test("sarscov2 single-end [fastq]") { when { - params { - outdir = "$outputDir" - } process { """ - input[0] = [ + input[0] = Channel.of([ [ id: 'test', single_end:true ], - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) """ } } @@ -28,82 +24,189 @@ nextflow_process { then { assertAll ( { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. // looks like this:
Mon 2 Oct 2023
test.gz
// https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, - { assert path(process.out.html.get(0).get(1)).getText().contains("") }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 paired-end [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 interleaved [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } ) } } -// TODO -// // -// // Test with paired-end data -// // -// workflow test_fastqc_paired_end { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with interleaved data -// // -// workflow test_fastqc_interleaved { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with bam data -// // -// workflow test_fastqc_bam { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with multiple samples -// // -// workflow test_fastqc_multiple { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with custom prefix -// // -// workflow test_fastqc_custom_prefix { -// input = [ -// [ id:'mysample', single_end:true ], // meta map -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } + + test("sarscov2 paired-end [bam]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 multiple [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, + { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, + { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + { assert path(process.out.html[0][1][2]).text.contains("") }, + { assert path(process.out.html[0][1][3]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 custom_prefix") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastq] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.html.collect { file(it[1]).getName() } + + process.out.zip.collect { file(it[1]).getName() } + + process.out.versions ).match() } + ) + } + } + } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 636a32ce..5d624bb8 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,10 +1,20 @@ { + "sarscov2 single-end [fastq] - stub": { + "content": [ + [ + "test.html", + "test.zip", + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2024-01-17T18:40:57.254299" + }, "versions": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2023-10-09T23:40:54+0000" + "timestamp": "2024-01-17T18:36:50.033627" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index bc0bdb5b..7625b752 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.18 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 00cc48d2..1b9f7c43 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : - 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -43,7 +43,7 @@ process MULTIQC { stub: """ - touch multiqc_data + mkdir multiqc_data touch multiqc_plots touch multiqc_report.html diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f1aa660e..45a9bc35 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,4 +1,3 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index c2dad217..d0438eda 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -7,12 +7,9 @@ nextflow_process { tag "modules_nfcore" tag "multiqc" - test("MULTIQC: FASTQC") { + test("sarscov2 single-end [fastqc]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -26,20 +23,17 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } ) } } - test("MULTIQC: FASTQC and a config file") { + test("sarscov2 single-end [fastqc] [config]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -53,9 +47,35 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastqc] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.report.collect { file(it).getName() } + + process.out.data.collect { file(it).getName() } + + process.out.plots.collect { file(it).getName() } + + process.out.versions ).match() } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap new file mode 100644 index 00000000..d37e7304 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -0,0 +1,21 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:02:49.911994" + }, + "sarscov2 single-end [fastqc] - stub": { + "content": [ + [ + "multiqc_report.html", + "multiqc_data", + "multiqc_plots", + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:03:14.524346" + } +} \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index 9e5ac5da..b52cb3ab 100644 --- a/nextflow.config +++ b/nextflow.config @@ -71,7 +71,7 @@ try { } // Load nf-core/rnafusion custom profiles from different institutions. -// Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs! +// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs! // try { // includeConfig "${params.custom_config_base}/pipeline/rnafusion.config" // } catch (Exception e) { @@ -91,6 +91,7 @@ profiles { podman.enabled = false shifter.enabled = false charliecloud.enabled = false + channels = ['conda-forge', 'bioconda', 'defaults'] apptainer.enabled = false } mamba { diff --git a/pyproject.toml b/pyproject.toml index 0d62beb6..7d08e1c8 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,10 +1,13 @@ -# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. +# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.black] +[tool.ruff] line-length = 120 -target_version = ["py37", "py38", "py39", "py310"] +target-version = "py38" +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] +cache-dir = "~/.cache/ruff" -[tool.isort] -profile = "black" -known_first_party = ["nf_core"] -multi_line_output = 3 +[tool.ruff.isort] +known-first-party = ["nf_core"] + +[tool.ruff.per-file-ignores] +"__init__.py" = ["E402", "F401"] diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf index 686acab7..027a8b38 100644 --- a/workflows/rnafusion.nf +++ b/workflows/rnafusion.nf @@ -127,6 +127,13 @@ workflow.onComplete { } } +workflow.onError { + if (workflow.errorReport.contains("Process requirement exceeds available memory")) { + println("🛑 Default resources exceed availability 🛑 ") + println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") + } +} + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ THE END From 887624c641342d5e8c8cc373cd26d92f3c9423a0 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Wed, 31 Jan 2024 10:33:22 +0100 Subject: [PATCH 09/64] update changelog --- CHANGELOG.md | 2 ++ 1 file changed, 2 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index ef0b02d9..8ef345a3 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -9,6 +9,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Changed +- Update to nf-tools 2.11.1 + ### Fixed - fix VCF_COLLECT handling when a tool is absent from FUSIONREPORT report From 901fa94e0845f6d6f02667c645c5954c377614b2 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 2 Feb 2024 09:16:21 +0100 Subject: [PATCH 10/64] add dump and rewrite channel --- subworkflows/local/fusioninspector_workflow.nf | 10 ++++++++-- 1 file changed, 8 insertions(+), 2 deletions(-) diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf index 7a31d7cd..d3bebbaf 100644 --- a/subworkflows/local/fusioninspector_workflow.nf +++ b/subworkflows/local/fusioninspector_workflow.nf @@ -29,6 +29,8 @@ workflow FUSIONINSPECTOR_WORKFLOW { fusions: it[1].size() > 0 } + ch_fusion_list.fusions.dump(tag:'before_cat') + if (params.whitelist) { ch_whitelist = ch_fusion_list.fusions.combine(Channel.value(file(params.whitelist, checkIfExists:true))) .map { meta, fusions, whitelist -> [ meta, [fusions, whitelist] ] } @@ -36,10 +38,14 @@ workflow FUSIONINSPECTOR_WORKFLOW { CAT_CAT(ch_whitelist) // fusioninspector takes care of possible duplicates ch_versions = ch_versions.mix(CAT_CAT.out.versions) - ch_fusion_list.fusions = CAT_CAT.out.file_out + ch_reads_fusion = reads.join(CAT_CAT.out.file_out ) + ch_reads_fusion.dump(tag:'with_cat') } + else { + ch_reads_fusion = reads.join(ch_fusion_list.fusions ) + ch_reads_fusion.dump(tag:'without_cat') - ch_reads_fusion = reads.join(ch_fusion_list.fusions ) + } FUSIONINSPECTOR( ch_reads_fusion, index) ch_versions = ch_versions.mix(FUSIONINSPECTOR.out.versions) From c37fe98c19878d1cac2d0e223932e049c08dddab Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 2 Feb 2024 11:22:41 +0100 Subject: [PATCH 11/64] fix vcf_collect when fusioninspector output is empty but fusionreport is not --- bin/vcf_collect.py | 104 +++++++++++++++++++++++++++++++++------------ 1 file changed, 77 insertions(+), 27 deletions(-) diff --git a/bin/vcf_collect.py b/bin/vcf_collect.py index 2c25574f..9da5f5c4 100755 --- a/bin/vcf_collect.py +++ b/bin/vcf_collect.py @@ -43,7 +43,6 @@ def vcf_collect( .reset_index() ) hgnc_df = build_hgnc_dataframe(hgnc) - df_symbol = merged_df[merged_df["Left_ensembl_gene_id"].isna()] df_not_symbol = merged_df[merged_df["Left_ensembl_gene_id"].notna()] @@ -66,7 +65,9 @@ def vcf_collect( gtf_df = build_gtf_dataframe(gtf) all_df = df.merge(gtf_df, how="left", left_on="CDS_LEFT_ID", right_on="Transcript_id") - all_df[["PosA", "orig_start", "orig_end"]] = all_df[["PosA", "orig_start", "orig_end"]].fillna(0).astype(int) + all_df[["PosA", "orig_start", "orig_end"]] = ( + all_df[["PosA", "orig_start", "orig_end"]].fillna(0).astype(int) + ) all_df = all_df[ ((all_df["PosA"] >= all_df["orig_start"]) & (all_df["PosA"] <= all_df["orig_end"])) @@ -76,7 +77,9 @@ def vcf_collect( all_df.replace("", np.nan, inplace=True) all_df = all_df.drop_duplicates() - all_df[["exon_number", "transcript_version"]] = all_df[["exon_number", "transcript_version"]].replace(0, np.nan) + all_df[["exon_number", "transcript_version"]] = all_df[ + ["exon_number", "transcript_version"] + ].replace(0, np.nan) # Fill non-empty values within each group for 'exon_number' and 'transcript_version' all_df["exon_number"] = all_df.groupby("PosA")["exon_number"].transform( lambda x: x.fillna(method="ffill").fillna(method="bfill") @@ -115,9 +118,14 @@ def vcf_collect( "annots", ] ].drop_duplicates() + all_df["CDS_RIGHT_ID"] = all_df["CDS_RIGHT_ID"].astype('str') all_df = all_df.merge(gtf_df, how="left", left_on="CDS_RIGHT_ID", right_on="Transcript_id") - all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].fillna(0) - all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].astype(int) + all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].fillna( + 0 + ) + all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].astype( + int + ) all_df = all_df[ ((all_df["PosB"] >= all_df["orig_start"]) & (all_df["PosB"] <= all_df["orig_end"])) | ((all_df["orig_start"] == 0) & (all_df["orig_end"] == 0)) @@ -126,7 +134,9 @@ def vcf_collect( all_df[["PosA", "PosB"]] = all_df[["PosA", "PosB"]].replace(0, np.nan) all_df = all_df.replace("", np.nan) - all_df[["exon_number", "transcript_version"]] = all_df[["exon_number", "transcript_version"]].replace(0, np.nan) + all_df[["exon_number", "transcript_version"]] = all_df[ + ["exon_number", "transcript_version"] + ].replace(0, np.nan) # Fill non-empty values within each group for 'exon_number' and 'transcript_version' all_df["exon_number"] = all_df.groupby("PosB")["exon_number"].transform( lambda x: x.fillna(method="ffill").fillna(method="bfill") @@ -212,7 +222,9 @@ def parse_args(argv=None): type=Path, help="HGNC database.", ) - parser.add_argument("--sample", metavar="SAMPLE", type=Path, help="Sample name.", default="Sample") + parser.add_argument( + "--sample", metavar="SAMPLE", type=Path, help="Sample name.", default="Sample" + ) parser.add_argument( "--out", metavar="OUT", @@ -272,15 +284,41 @@ def build_fusioninspector_dataframe(file: str) -> pd.DataFrame: """ df = pd.read_csv(file, sep="\t") df = df.rename(columns={"#FusionName": "FUSION"}) - df[["ChromosomeA", "PosA", "Strand1"]] = df["LeftBreakpoint"].str.split(":", expand=True) - df[["ChromosomeB", "PosB", "Strand2"]] = df["RightBreakpoint"].str.split(":", expand=True) - df[["LeftGeneName", "Left_ensembl_gene_id"]] = df["LeftGene"].str.split("^", expand=True) - df[["RightGeneName", "Right_ensembl_gene_id"]] = df["RightGene"].str.split("^", expand=True) - df["annots"] = ( - df["annots"] - .apply(convert_to_list) - .apply(lambda x: ",".join(map(str, x)) if isinstance(x, list) else str(x) if pd.notna(x) else "") - ) + if not (df.empty): + df[["ChromosomeA", "PosA", "Strand1"]] = df["LeftBreakpoint"].str.split(":", expand=True) + df[["ChromosomeB", "PosB", "Strand2"]] = df["RightBreakpoint"].str.split(":", expand=True) + df[["LeftGeneName", "Left_ensembl_gene_id"]] = df["LeftGene"].str.split("^", expand=True) + df[["RightGeneName", "Right_ensembl_gene_id"]] = df["RightGene"].str.split("^", expand=True) + df["annots"] = ( + df["annots"] + .apply(convert_to_list) + .apply( + lambda x: ",".join(map(str, x)) + if isinstance(x, list) + else str(x) + if pd.notna(x) + else "" + ) + ) + else: + for i in [ + "ChromosomeA", + "Strand1", + "ChromosomeB", + "Strand2", + "LeftGeneName", + "Left_ensembl_gene_id", + "RightGeneName", + "Right_ensembl_gene_id", + "annots", + ]: + df[i] = "" + for j in [ + "PosA", + "PosB", + ]: + df[j] = np.nan + return df.set_index(["FUSION"]) @@ -315,8 +353,8 @@ def read_build_fusionreport(fusionreport_file: str) -> pd.DataFrame: """ with open(fusionreport_file) as f: from_html = [line.split('rows": [')[1] for line in f if 'name="fusion_list' in line] - expression = from_html[0].split('], "tool')[0] - fusion_report = pd.DataFrame.from_dict(ast.literal_eval(expression)) + expression = ast.literal_eval(from_html[0].split('], "tool')[0]) + fusion_report = pd.DataFrame.from_dict({k: [v] for k, v in expression.items()}) if not "arriba" in fusion_report.columns: fusion_report["arriba"] = "" if not "fusioncatcher" in fusion_report.columns: @@ -339,9 +377,9 @@ def read_build_fusionreport(fusionreport_file: str) -> pd.DataFrame: fusion_report["FOUND_DB"] = fusion_report["FOUND_DB"].apply(lambda x: ",".join(x)) fusion_report[["GeneA", "GeneB"]] = fusion_report["FUSION"].str.split("--", expand=True) - return fusion_report[["FUSION", "GeneA", "GeneB", "TOOLS_HITS", "SCORE", "FOUND_DB", "FOUND_IN"]].set_index( - ["FUSION"] - ) + return fusion_report[ + ["FUSION", "GeneA", "GeneB", "TOOLS_HITS", "SCORE", "FOUND_DB", "FOUND_IN"] + ].set_index(["FUSION"]) def read_fusionreport_csv(file: str) -> pd.DataFrame: @@ -350,7 +388,9 @@ def read_fusionreport_csv(file: str) -> pd.DataFrame: for column in columns_to_iterate: if column not in df.columns: df[column] = "" - df[["starfusion", "arriba", "fusioncatcher"]] = df[["starfusion", "arriba", "fusioncatcher"]].astype("str") + df[["starfusion", "arriba", "fusioncatcher"]] = df[ + ["starfusion", "arriba", "fusioncatcher"] + ].astype("str") for index, row in df.iterrows(): for column in columns_to_iterate: cell_value = row[column] @@ -378,7 +418,9 @@ def read_fusionreport_csv(file: str) -> pd.DataFrame: df[["GeneA", "GeneB"]] = df["Fusion"].str.split("--", expand=True) df = df.set_index("Fusion") df.to_csv("tmp.csv") - return df[["GeneA", "GeneB", "ChromosomeA", "PosA", "StrandA", "ChromosomeB", "PosB", "StrandB"]] + return df[ + ["GeneA", "GeneB", "ChromosomeA", "PosA", "StrandA", "ChromosomeB", "PosB", "StrandB"] + ] def column_manipulation(df: pd.DataFrame) -> pd.DataFrame: @@ -405,7 +447,9 @@ def column_manipulation(df: pd.DataFrame) -> pd.DataFrame: df["Left_exon_number"] = df["Left_exon_number"].fillna(0).astype(int).astype(str) df["Right_exon_number"] = df["Right_exon_number"].fillna(0).astype(int).astype(str) df["Left_transcript_version"] = df["Left_transcript_version"].fillna(0).astype(int).astype(str) - df["Right_transcript_version"] = df["Right_transcript_version"].fillna(0).astype(int).astype(str) + df["Right_transcript_version"] = ( + df["Right_transcript_version"].fillna(0).astype(int).astype(str) + ) df["PosA"] = df["PosA"].fillna(0).astype(int).astype(str) df["PosB"] = df["PosB"].fillna(0).astype(int).astype(str) df["PROT_FUSION_TYPE"] = df["PROT_FUSION_TYPE"].replace(".", "nan") @@ -432,7 +476,9 @@ def column_manipulation(df: pd.DataFrame) -> pd.DataFrame: f"EXON_NUMBER_A={row['Left_exon_number']};EXON_NUMBER_B={row['Right_exon_number']};" f"ANNOTATIONS={row['annots']}" ) - df.loc[index, "Sample"] = f"./1:{row['JunctionReadCount']}:{row['SpanningFragCount']}:{row['FFPM']}" + df.loc[ + index, "Sample" + ] = f"./1:{row['JunctionReadCount']}:{row['SpanningFragCount']}:{row['FFPM']}" return df @@ -477,7 +523,9 @@ def build_gtf_dataframe(file: str) -> pd.DataFrame: """ df = pd.read_csv(file, sep="\t") df[["fusion_dump", "Transcript_id"]] = df["transcript_id"].str.split("^", expand=True) - df[["orig_chromosome", "orig_start", "orig_end", "orig_dir"]] = df["orig_coord_info"].str.split(",", expand=True) + df[["orig_chromosome", "orig_start", "orig_end", "orig_dir"]] = df["orig_coord_info"].str.split( + ",", expand=True + ) return df[["Transcript_id", "transcript_version", "exon_number", "orig_start", "orig_end"]] @@ -491,7 +539,9 @@ def main(argv=None): or not args.fusionreport_csv or not args.hgnc ): - logger.error(f"The given input file {args.fusioninspector} or {args.fusionreport} was not found!") + logger.error( + f"The given input file {args.fusioninspector} or {args.fusionreport} was not found!" + ) sys.exit(2) vcf_collect( args.fusioninspector, From a6659dac892a335d5bfd4f2b810b4fabeef9b48b Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 2 Feb 2024 11:24:24 +0100 Subject: [PATCH 12/64] update changelog --- CHANGELOG.md | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 8ef345a3..d592cefc 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -9,11 +9,12 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Changed -- Update to nf-tools 2.11.1 +- Update to nf-tools 2.11.1 [#457] (https://github.com/nf-core/rnafusion/pull/457) ### Fixed -- fix VCF_COLLECT handling when a tool is absent from FUSIONREPORT report +- fix VCF_COLLECT handling when a tool is absent from FUSIONREPORT report [#458](https://github.com/nf-core/rnafusion/pull/458) +- fix VCF_COLLECT when fusioninspector output is empty but fusionreport is not ### Removed From b67a751bbffcef5a2f80c02f9ce4c073e8a4aa0d Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 2 Feb 2024 11:26:57 +0100 Subject: [PATCH 13/64] update changelog --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index d592cefc..6468aff6 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -14,7 +14,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Fixed - fix VCF_COLLECT handling when a tool is absent from FUSIONREPORT report [#458](https://github.com/nf-core/rnafusion/pull/458) -- fix VCF_COLLECT when fusioninspector output is empty but fusionreport is not +- fix VCF_COLLECT when fusioninspector output is empty but fusionreport is not [#465](https://github.com/nf-core/rnafusion/pull/465) ### Removed From 26e492c3140ac3f6e43cd3dbd2ff332611cb511a Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 2 Feb 2024 11:35:18 +0100 Subject: [PATCH 14/64] black --- bin/vcf_collect.py | 64 ++++++++++++---------------------------------- 1 file changed, 17 insertions(+), 47 deletions(-) diff --git a/bin/vcf_collect.py b/bin/vcf_collect.py index 9da5f5c4..7e988c1e 100755 --- a/bin/vcf_collect.py +++ b/bin/vcf_collect.py @@ -65,9 +65,7 @@ def vcf_collect( gtf_df = build_gtf_dataframe(gtf) all_df = df.merge(gtf_df, how="left", left_on="CDS_LEFT_ID", right_on="Transcript_id") - all_df[["PosA", "orig_start", "orig_end"]] = ( - all_df[["PosA", "orig_start", "orig_end"]].fillna(0).astype(int) - ) + all_df[["PosA", "orig_start", "orig_end"]] = all_df[["PosA", "orig_start", "orig_end"]].fillna(0).astype(int) all_df = all_df[ ((all_df["PosA"] >= all_df["orig_start"]) & (all_df["PosA"] <= all_df["orig_end"])) @@ -77,9 +75,7 @@ def vcf_collect( all_df.replace("", np.nan, inplace=True) all_df = all_df.drop_duplicates() - all_df[["exon_number", "transcript_version"]] = all_df[ - ["exon_number", "transcript_version"] - ].replace(0, np.nan) + all_df[["exon_number", "transcript_version"]] = all_df[["exon_number", "transcript_version"]].replace(0, np.nan) # Fill non-empty values within each group for 'exon_number' and 'transcript_version' all_df["exon_number"] = all_df.groupby("PosA")["exon_number"].transform( lambda x: x.fillna(method="ffill").fillna(method="bfill") @@ -118,14 +114,10 @@ def vcf_collect( "annots", ] ].drop_duplicates() - all_df["CDS_RIGHT_ID"] = all_df["CDS_RIGHT_ID"].astype('str') + all_df["CDS_RIGHT_ID"] = all_df["CDS_RIGHT_ID"].astype("str") all_df = all_df.merge(gtf_df, how="left", left_on="CDS_RIGHT_ID", right_on="Transcript_id") - all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].fillna( - 0 - ) - all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].astype( - int - ) + all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].fillna(0) + all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].astype(int) all_df = all_df[ ((all_df["PosB"] >= all_df["orig_start"]) & (all_df["PosB"] <= all_df["orig_end"])) | ((all_df["orig_start"] == 0) & (all_df["orig_end"] == 0)) @@ -134,9 +126,7 @@ def vcf_collect( all_df[["PosA", "PosB"]] = all_df[["PosA", "PosB"]].replace(0, np.nan) all_df = all_df.replace("", np.nan) - all_df[["exon_number", "transcript_version"]] = all_df[ - ["exon_number", "transcript_version"] - ].replace(0, np.nan) + all_df[["exon_number", "transcript_version"]] = all_df[["exon_number", "transcript_version"]].replace(0, np.nan) # Fill non-empty values within each group for 'exon_number' and 'transcript_version' all_df["exon_number"] = all_df.groupby("PosB")["exon_number"].transform( lambda x: x.fillna(method="ffill").fillna(method="bfill") @@ -222,9 +212,7 @@ def parse_args(argv=None): type=Path, help="HGNC database.", ) - parser.add_argument( - "--sample", metavar="SAMPLE", type=Path, help="Sample name.", default="Sample" - ) + parser.add_argument("--sample", metavar="SAMPLE", type=Path, help="Sample name.", default="Sample") parser.add_argument( "--out", metavar="OUT", @@ -292,13 +280,7 @@ def build_fusioninspector_dataframe(file: str) -> pd.DataFrame: df["annots"] = ( df["annots"] .apply(convert_to_list) - .apply( - lambda x: ",".join(map(str, x)) - if isinstance(x, list) - else str(x) - if pd.notna(x) - else "" - ) + .apply(lambda x: ",".join(map(str, x)) if isinstance(x, list) else str(x) if pd.notna(x) else "") ) else: for i in [ @@ -377,9 +359,9 @@ def read_build_fusionreport(fusionreport_file: str) -> pd.DataFrame: fusion_report["FOUND_DB"] = fusion_report["FOUND_DB"].apply(lambda x: ",".join(x)) fusion_report[["GeneA", "GeneB"]] = fusion_report["FUSION"].str.split("--", expand=True) - return fusion_report[ - ["FUSION", "GeneA", "GeneB", "TOOLS_HITS", "SCORE", "FOUND_DB", "FOUND_IN"] - ].set_index(["FUSION"]) + return fusion_report[["FUSION", "GeneA", "GeneB", "TOOLS_HITS", "SCORE", "FOUND_DB", "FOUND_IN"]].set_index( + ["FUSION"] + ) def read_fusionreport_csv(file: str) -> pd.DataFrame: @@ -388,9 +370,7 @@ def read_fusionreport_csv(file: str) -> pd.DataFrame: for column in columns_to_iterate: if column not in df.columns: df[column] = "" - df[["starfusion", "arriba", "fusioncatcher"]] = df[ - ["starfusion", "arriba", "fusioncatcher"] - ].astype("str") + df[["starfusion", "arriba", "fusioncatcher"]] = df[["starfusion", "arriba", "fusioncatcher"]].astype("str") for index, row in df.iterrows(): for column in columns_to_iterate: cell_value = row[column] @@ -418,9 +398,7 @@ def read_fusionreport_csv(file: str) -> pd.DataFrame: df[["GeneA", "GeneB"]] = df["Fusion"].str.split("--", expand=True) df = df.set_index("Fusion") df.to_csv("tmp.csv") - return df[ - ["GeneA", "GeneB", "ChromosomeA", "PosA", "StrandA", "ChromosomeB", "PosB", "StrandB"] - ] + return df[["GeneA", "GeneB", "ChromosomeA", "PosA", "StrandA", "ChromosomeB", "PosB", "StrandB"]] def column_manipulation(df: pd.DataFrame) -> pd.DataFrame: @@ -447,9 +425,7 @@ def column_manipulation(df: pd.DataFrame) -> pd.DataFrame: df["Left_exon_number"] = df["Left_exon_number"].fillna(0).astype(int).astype(str) df["Right_exon_number"] = df["Right_exon_number"].fillna(0).astype(int).astype(str) df["Left_transcript_version"] = df["Left_transcript_version"].fillna(0).astype(int).astype(str) - df["Right_transcript_version"] = ( - df["Right_transcript_version"].fillna(0).astype(int).astype(str) - ) + df["Right_transcript_version"] = df["Right_transcript_version"].fillna(0).astype(int).astype(str) df["PosA"] = df["PosA"].fillna(0).astype(int).astype(str) df["PosB"] = df["PosB"].fillna(0).astype(int).astype(str) df["PROT_FUSION_TYPE"] = df["PROT_FUSION_TYPE"].replace(".", "nan") @@ -476,9 +452,7 @@ def column_manipulation(df: pd.DataFrame) -> pd.DataFrame: f"EXON_NUMBER_A={row['Left_exon_number']};EXON_NUMBER_B={row['Right_exon_number']};" f"ANNOTATIONS={row['annots']}" ) - df.loc[ - index, "Sample" - ] = f"./1:{row['JunctionReadCount']}:{row['SpanningFragCount']}:{row['FFPM']}" + df.loc[index, "Sample"] = f"./1:{row['JunctionReadCount']}:{row['SpanningFragCount']}:{row['FFPM']}" return df @@ -523,9 +497,7 @@ def build_gtf_dataframe(file: str) -> pd.DataFrame: """ df = pd.read_csv(file, sep="\t") df[["fusion_dump", "Transcript_id"]] = df["transcript_id"].str.split("^", expand=True) - df[["orig_chromosome", "orig_start", "orig_end", "orig_dir"]] = df["orig_coord_info"].str.split( - ",", expand=True - ) + df[["orig_chromosome", "orig_start", "orig_end", "orig_dir"]] = df["orig_coord_info"].str.split(",", expand=True) return df[["Transcript_id", "transcript_version", "exon_number", "orig_start", "orig_end"]] @@ -539,9 +511,7 @@ def main(argv=None): or not args.fusionreport_csv or not args.hgnc ): - logger.error( - f"The given input file {args.fusioninspector} or {args.fusionreport} was not found!" - ) + logger.error(f"The given input file {args.fusioninspector} or {args.fusionreport} was not found!") sys.exit(2) vcf_collect( args.fusioninspector, From b2db95e89dfdc4577728a11b6fd3156280221790 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 2 Feb 2024 11:42:35 +0100 Subject: [PATCH 15/64] harmonise ensembl_version default to 102 --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 29ae6288..28ed55fc 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -80,7 +80,7 @@ "type": "integer", "fa_icon": "far fa-file-code", "description": "ensembl version", - "default": 105 + "default": 102 }, "starfusion_build": { "type": "boolean", From 30604e2efbcad83845f5dc1ff7746e4b4e6e4aa2 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 2 Feb 2024 11:43:12 +0100 Subject: [PATCH 16/64] prettier --- .github/workflows/download_pipeline.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 8611458a..8a330045 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -64,4 +64,4 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results From 8c243b3de18a4ac1b9d706bf67b7bebe0490e8f6 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 2 Feb 2024 11:52:11 +0100 Subject: [PATCH 17/64] prettier with correct version --- .devcontainer/devcontainer.json | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4a9bc5c7..4ecfbfe3 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -18,11 +18,11 @@ "python.linting.flake8Path": "/opt/conda/bin/flake8", "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint", + "python.linting.pylintPath": "/opt/conda/bin/pylint" }, // Add the IDs of extensions you want installed when the container is created. - "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"], - }, - }, + "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] + } + } } From 239345ab67d117c7a7024e88585dc2a60ae86e22 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 2 Feb 2024 12:06:36 +0100 Subject: [PATCH 18/64] fix logos --- assets/nf-core-rnafusion_logo_light.png | Bin 75831 -> 75832 bytes docs/images/nf-core-rnafusion_logo_dark.png | Bin 27749 -> 27732 bytes docs/images/nf-core-rnafusion_logo_light.png | Bin 23933 -> 23936 bytes 3 files changed, 0 insertions(+), 0 deletions(-) diff --git a/assets/nf-core-rnafusion_logo_light.png b/assets/nf-core-rnafusion_logo_light.png index fce712782716cb146bd3b4147dc9821244151318..96e1a4e813d8d5e01804e2a43cc3ee6349871815 100644 GIT binary patch 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CHANGELOG.md | 1 + subworkflows/local/fusioninspector_workflow.nf | 4 ---- 2 files changed, 1 insertion(+), 4 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 8ef345a3..0ce69eb7 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -14,6 +14,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Fixed - fix VCF_COLLECT handling when a tool is absent from FUSIONREPORT report +- fix bug when using parameter "whitelist" [#466](https://github.com/nf-core/rnafusion/pull/466) ### Removed diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf index d3bebbaf..a826fbfc 100644 --- a/subworkflows/local/fusioninspector_workflow.nf +++ b/subworkflows/local/fusioninspector_workflow.nf @@ -37,14 +37,10 @@ workflow FUSIONINSPECTOR_WORKFLOW { CAT_CAT(ch_whitelist) // fusioninspector takes care of possible duplicates ch_versions = ch_versions.mix(CAT_CAT.out.versions) - ch_reads_fusion = reads.join(CAT_CAT.out.file_out ) - ch_reads_fusion.dump(tag:'with_cat') } else { ch_reads_fusion = reads.join(ch_fusion_list.fusions ) - ch_reads_fusion.dump(tag:'without_cat') - } FUSIONINSPECTOR( ch_reads_fusion, index) From 2c75e6005445c3170e07bbc2fa8a9dbf54f7d3ad Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 20 Feb 2024 15:30:09 +0000 Subject: [PATCH 22/64] Template update for nf-core/tools version 2.13 --- .editorconfig | 9 +- .github/workflows/awsfulltest.yml | 4 +- .github/workflows/awstest.yml | 4 +- .github/workflows/branch.yml | 2 +- .github/workflows/ci.yml | 7 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 17 +- .github/workflows/linting.yml | 12 +- .github/workflows/linting_comment.yml | 4 +- .github/workflows/release-announcements.yml | 11 +- README.md | 5 +- assets/multiqc_config.yml | 2 + assets/schema_input.json | 21 +- bin/check_samplesheet.py | 259 ----------- conf/modules.config | 8 - lib/NfcoreTemplate.groovy | 356 -------------- lib/Utils.groovy | 47 -- lib/WorkflowMain.groovy | 77 --- lib/WorkflowRnafusion.groovy | 122 ----- main.nf | 100 ++-- modules.json | 28 +- modules/local/samplesheet_check.nf | 31 -- .../dumpsoftwareversions/environment.yml | 7 - .../custom/dumpsoftwareversions/main.nf | 24 - .../custom/dumpsoftwareversions/meta.yml | 37 -- .../templates/dumpsoftwareversions.py | 102 ---- .../dumpsoftwareversions/tests/main.nf.test | 43 -- .../tests/main.nf.test.snap | 33 -- .../dumpsoftwareversions/tests/tags.yml | 2 - modules/nf-core/fastqc/tests/main.nf.test | 14 +- .../nf-core/fastqc/tests/main.nf.test.snap | 76 ++- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- modules/nf-core/multiqc/tests/main.nf.test | 13 +- .../nf-core/multiqc/tests/main.nf.test.snap | 32 +- nextflow.config | 5 +- nextflow_schema.json | 1 + pyproject.toml | 8 +- subworkflows/local/input_check.nf | 44 -- .../utils_nfcore_rnafusion_pipeline/main.nf | 247 ++++++++++ .../nf-core/utils_nextflow_pipeline/main.nf | 126 +++++ .../nf-core/utils_nextflow_pipeline/meta.yml | 38 ++ .../tests/main.function.nf.test | 54 +++ .../tests/main.function.nf.test.snap | 12 + .../tests/main.workflow.nf.test | 123 +++++ .../tests/nextflow.config | 9 + .../utils_nextflow_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfcore_pipeline/main.nf | 440 ++++++++++++++++++ .../nf-core/utils_nfcore_pipeline/meta.yml | 24 + .../tests/main.function.nf.test | 134 ++++++ .../tests/main.function.nf.test.snap | 138 ++++++ .../tests/main.workflow.nf.test | 29 ++ .../tests/main.workflow.nf.test.snap | 15 + .../tests/nextflow.config | 9 + .../utils_nfcore_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfvalidation_plugin/main.nf | 62 +++ .../utils_nfvalidation_plugin/meta.yml | 44 ++ .../tests/main.nf.test | 200 ++++++++ .../tests/nextflow_schema.json | 96 ++++ .../utils_nfvalidation_plugin/tests/tags.yml | 2 + workflows/rnafusion.nf | 133 ++---- 61 files changed, 2108 insertions(+), 1406 deletions(-) delete mode 100755 bin/check_samplesheet.py delete mode 100755 lib/NfcoreTemplate.groovy delete mode 100644 lib/Utils.groovy delete mode 100755 lib/WorkflowMain.groovy delete mode 100755 lib/WorkflowRnafusion.groovy delete mode 100644 modules/local/samplesheet_check.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml delete mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml delete mode 100644 subworkflows/local/input_check.nf create mode 100644 subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml diff --git a/.editorconfig b/.editorconfig index 9b990088..dd9ffa53 100644 --- a/.editorconfig +++ b/.editorconfig @@ -18,7 +18,12 @@ end_of_line = unset insert_final_newline = unset trim_trailing_whitespace = unset indent_style = unset -indent_size = unset +[/subworkflows/nf-core/**] +charset = unset +end_of_line = unset +insert_final_newline = unset +trim_trailing_whitespace = unset +indent_style = unset [/assets/email*] indent_size = unset @@ -28,5 +33,5 @@ indent_size = unset indent_style = unset # ignore python -[*.{py}] +[*.{py,md}] indent_style = unset diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index a4b6b4d3..b46a4819 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v2 + uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@v4 + - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index cf29b8fd..104df35b 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,7 +12,7 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v2 + uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@v4 + - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 476d72a8..19704d23 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v2 + uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 31823f13..bdbc1fcb 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,13 +28,16 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 with: version: "${{ matrix.NXF_VER }}" + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + - name: Run pipeline with test data # TODO nf-core: You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index e37cfda5..0b6b1f27 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v9 + - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 8611458a..f823210d 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -6,6 +6,11 @@ name: Test successful pipeline download with 'nf-core download' # - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. on: workflow_dispatch: + inputs: + testbranch: + description: "The specific branch you wish to utilize for the test execution of nf-core download." + required: true + default: "dev" pull_request: types: - opened @@ -23,13 +28,13 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" - - uses: eWaterCycle/setup-singularity@v7 + - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 with: singularity-version: 3.8.3 @@ -42,13 +47,13 @@ jobs: run: | echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} - echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} - name: Download the pipeline env: NXF_SINGULARITY_CACHEDIR: ./ run: | - nf-core download ${{ env.REPO_LOWERCASE }} \ + nf-core download ${{ env.REPO_LOWERCASE }} \ --revision ${{ env.REPO_BRANCH }} \ --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ --compress "none" \ @@ -64,4 +69,4 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 81cd098e..748b4311 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,10 +14,10 @@ jobs: pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Set up Python 3.11 - uses: actions/setup-python@v5 + uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: 3.11 cache: "pip" @@ -32,12 +32,12 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" @@ -60,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v4 + uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 147bcd10..b706875f 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v3 + uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 with: workflow: linting.yml workflow_conclusion: completed @@ -21,7 +21,7 @@ jobs: run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment - uses: marocchino/sticky-pull-request-comment@v2 + uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2 with: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} number: ${{ steps.pr_number.outputs.pr_number }} diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 21ac3f06..c3674af2 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -9,6 +9,11 @@ jobs: toot: runs-on: ubuntu-latest steps: + - name: get topics and convert to hashtags + id: get_topics + run: | + curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' > $GITHUB_OUTPUT + - uses: rzr/fediverse-action@master with: access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} @@ -20,11 +25,13 @@ jobs: Please see the changelog: ${{ github.event.release.html_url }} + ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics + send-tweet: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +63,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.1.0 + - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/README.md b/README.md index c48b6f3b..ca941ca5 100644 --- a/README.md +++ b/README.md @@ -4,8 +4,9 @@ nf-core/rnafusion -[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+CI%22) -[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/rnafusion/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) + +[![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml) +[![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index b8874305..c070b89c 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -11,3 +11,5 @@ report_section_order: order: -1002 export_plots: true + +disable_version_detection: true diff --git a/assets/schema_input.json b/assets/schema_input.json index aff11de1..fa630b6c 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -10,25 +10,22 @@ "sample": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Sample name must be provided and cannot contain spaces" + "errorMessage": "Sample name must be provided and cannot contain spaces", + "meta": ["id"] }, "fastq_1": { "type": "string", + "format": "file-path", + "exists": true, "pattern": "^\\S+\\.f(ast)?q\\.gz$", "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" }, "fastq_2": { - "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", - "anyOf": [ - { - "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$" - }, - { - "type": "string", - "maxLength": 0 - } - ] + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.f(ast)?q\\.gz$", + "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" } }, "required": ["sample", "fastq_1"] diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py deleted file mode 100755 index 4a758fe0..00000000 --- a/bin/check_samplesheet.py +++ /dev/null @@ -1,259 +0,0 @@ -#!/usr/bin/env python - - -"""Provide a command line tool to validate and transform tabular samplesheets.""" - - -import argparse -import csv -import logging -import sys -from collections import Counter -from pathlib import Path - -logger = logging.getLogger() - - -class RowChecker: - """ - Define a service that can validate and transform each given row. - - Attributes: - modified (list): A list of dicts, where each dict corresponds to a previously - validated and transformed row. The order of rows is maintained. - - """ - - VALID_FORMATS = ( - ".fq.gz", - ".fastq.gz", - ) - - def __init__( - self, - sample_col="sample", - first_col="fastq_1", - second_col="fastq_2", - single_col="single_end", - **kwargs, - ): - """ - Initialize the row checker with the expected column names. - - Args: - sample_col (str): The name of the column that contains the sample name - (default "sample"). - first_col (str): The name of the column that contains the first (or only) - FASTQ file path (default "fastq_1"). - second_col (str): The name of the column that contains the second (if any) - FASTQ file path (default "fastq_2"). - single_col (str): The name of the new column that will be inserted and - records whether the sample contains single- or paired-end sequencing - reads (default "single_end"). - - """ - super().__init__(**kwargs) - self._sample_col = sample_col - self._first_col = first_col - self._second_col = second_col - self._single_col = single_col - self._seen = set() - self.modified = [] - - def validate_and_transform(self, row): - """ - Perform all validations on the given row and insert the read pairing status. - - Args: - row (dict): A mapping from column headers (keys) to elements of that row - (values). - - """ - self._validate_sample(row) - self._validate_first(row) - self._validate_second(row) - self._validate_pair(row) - self._seen.add((row[self._sample_col], row[self._first_col])) - self.modified.append(row) - - def _validate_sample(self, row): - """Assert that the sample name exists and convert spaces to underscores.""" - if len(row[self._sample_col]) <= 0: - raise AssertionError("Sample input is required.") - # Sanitize samples slightly. - row[self._sample_col] = row[self._sample_col].replace(" ", "_") - - def _validate_first(self, row): - """Assert that the first FASTQ entry is non-empty and has the right format.""" - if len(row[self._first_col]) <= 0: - raise AssertionError("At least the first FASTQ file is required.") - self._validate_fastq_format(row[self._first_col]) - - def _validate_second(self, row): - """Assert that the second FASTQ entry has the right format if it exists.""" - if len(row[self._second_col]) > 0: - self._validate_fastq_format(row[self._second_col]) - - def _validate_pair(self, row): - """Assert that read pairs have the same file extension. Report pair status.""" - if row[self._first_col] and row[self._second_col]: - row[self._single_col] = False - first_col_suffix = Path(row[self._first_col]).suffixes[-2:] - second_col_suffix = Path(row[self._second_col]).suffixes[-2:] - if first_col_suffix != second_col_suffix: - raise AssertionError("FASTQ pairs must have the same file extensions.") - else: - row[self._single_col] = True - - def _validate_fastq_format(self, filename): - """Assert that a given filename has one of the expected FASTQ extensions.""" - if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): - raise AssertionError( - f"The FASTQ file has an unrecognized extension: {filename}\n" - f"It should be one of: {', '.join(self.VALID_FORMATS)}" - ) - - def validate_unique_samples(self): - """ - Assert that the combination of sample name and FASTQ filename is unique. - - In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the - number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. - - """ - if len(self._seen) != len(self.modified): - raise AssertionError("The pair of sample name and FASTQ must be unique.") - seen = Counter() - for row in self.modified: - sample = row[self._sample_col] - seen[sample] += 1 - row[self._sample_col] = f"{sample}_T{seen[sample]}" - - -def read_head(handle, num_lines=10): - """Read the specified number of lines from the current position in the file.""" - lines = [] - for idx, line in enumerate(handle): - if idx == num_lines: - break - lines.append(line) - return "".join(lines) - - -def sniff_format(handle): - """ - Detect the tabular format. - - Args: - handle (text file): A handle to a `text file`_ object. The read position is - expected to be at the beginning (index 0). - - Returns: - csv.Dialect: The detected tabular format. - - .. _text file: - https://docs.python.org/3/glossary.html#term-text-file - - """ - peek = read_head(handle) - handle.seek(0) - sniffer = csv.Sniffer() - dialect = sniffer.sniff(peek) - return dialect - - -def check_samplesheet(file_in, file_out): - """ - Check that the tabular samplesheet has the structure expected by nf-core pipelines. - - Validate the general shape of the table, expected columns, and each row. Also add - an additional column which records whether one or two FASTQ reads were found. - - Args: - file_in (pathlib.Path): The given tabular samplesheet. The format can be either - CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. - file_out (pathlib.Path): Where the validated and transformed samplesheet should - be created; always in CSV format. - - Example: - This function checks that the samplesheet follows the following structure, - see also the `viral recon samplesheet`_:: - - sample,fastq_1,fastq_2 - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, - - .. _viral recon samplesheet: - https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv - - """ - required_columns = {"sample", "fastq_1", "fastq_2"} - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_in.open(newline="") as in_handle: - reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) - # Validate the existence of the expected header columns. - if not required_columns.issubset(reader.fieldnames): - req_cols = ", ".join(required_columns) - logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") - sys.exit(1) - # Validate each row. - checker = RowChecker() - for i, row in enumerate(reader): - try: - checker.validate_and_transform(row) - except AssertionError as error: - logger.critical(f"{str(error)} On line {i + 2}.") - sys.exit(1) - checker.validate_unique_samples() - header = list(reader.fieldnames) - header.insert(1, "single_end") - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_out.open(mode="w", newline="") as out_handle: - writer = csv.DictWriter(out_handle, header, delimiter=",") - writer.writeheader() - for row in checker.modified: - writer.writerow(row) - - -def parse_args(argv=None): - """Define and immediately parse command line arguments.""" - parser = argparse.ArgumentParser( - description="Validate and transform a tabular samplesheet.", - epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", - ) - parser.add_argument( - "file_in", - metavar="FILE_IN", - type=Path, - help="Tabular input samplesheet in CSV or TSV format.", - ) - parser.add_argument( - "file_out", - metavar="FILE_OUT", - type=Path, - help="Transformed output samplesheet in CSV format.", - ) - parser.add_argument( - "-l", - "--log-level", - help="The desired log level (default WARNING).", - choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), - default="WARNING", - ) - return parser.parse_args(argv) - - -def main(argv=None): - """Coordinate argument parsing and program execution.""" - args = parse_args(argv) - logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") - if not args.file_in.is_file(): - logger.error(f"The given input file {args.file_in} was not found!") - sys.exit(2) - args.file_out.parent.mkdir(parents=True, exist_ok=True) - check_samplesheet(args.file_in, args.file_out) - - -if __name__ == "__main__": - sys.exit(main()) diff --git a/conf/modules.config b/conf/modules.config index d91c6aba..e3ea8fa6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -18,14 +18,6 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: SAMPLESHEET_CHECK { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - withName: FASTQC { ext.args = '--quiet' } diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy deleted file mode 100755 index e248e4c3..00000000 --- a/lib/NfcoreTemplate.groovy +++ /dev/null @@ -1,356 +0,0 @@ -// -// This file holds several functions used within the nf-core pipeline template. -// - -import org.yaml.snakeyaml.Yaml -import groovy.json.JsonOutput -import nextflow.extension.FilesEx - -class NfcoreTemplate { - - // - // Check AWS Batch related parameters have been specified correctly - // - public static void awsBatch(workflow, params) { - if (workflow.profile.contains('awsbatch')) { - // Check params.awsqueue and params.awsregion have been set if running on AWSBatch - assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - } - } - - // - // Warn if a -profile or Nextflow config has not been provided to run the pipeline - // - public static void checkConfigProvided(workflow, log) { - if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " - } - } - - // - // Generate version string - // - public static String version(workflow) { - String version_string = "" - - if (workflow.manifest.version) { - def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' - version_string += "${prefix_v}${workflow.manifest.version}" - } - - if (workflow.commitId) { - def git_shortsha = workflow.commitId.substring(0, 7) - version_string += "-g${git_shortsha}" - } - - return version_string - } - - // - // Construct and send completion email - // - public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['Date Started'] = workflow.start - misc_fields['Date Completed'] = workflow.complete - misc_fields['Pipeline script file path'] = workflow.scriptFile - misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build - misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - def email_fields = [:] - email_fields['version'] = NfcoreTemplate.version(workflow) - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary << misc_fields - - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { - if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" - } - mqc_report = mqc_report[0] - } - } - } catch (all) { - if (multiqc_report) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" - } - } - - // Check if we are only sending emails on failure - def email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - Map colors = logColours(params.monochrome_logs) - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") - sendmail_tf.withWriter { w -> w << sendmail_html } - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" - } - } - - // Write summary e-mail HTML to a file - def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); - output_hf.delete() - - // Write summary e-mail TXT to a file - def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); - output_tf.delete() - } - - // - // Construct and send a notification to a web server as JSON - // e.g. Microsoft Teams and Slack - // - public static void IM_notification(workflow, params, summary_params, projectDir, log) { - def hook_url = params.hook_url - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp - - def msg_fields = [:] - msg_fields['version'] = NfcoreTemplate.version(workflow) - msg_fields['runName'] = workflow.runName - msg_fields['success'] = workflow.success - msg_fields['dateComplete'] = workflow.complete - msg_fields['duration'] = workflow.duration - msg_fields['exitStatus'] = workflow.exitStatus - msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - msg_fields['errorReport'] = (workflow.errorReport ?: 'None') - msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") - msg_fields['projectDir'] = workflow.projectDir - msg_fields['summary'] = summary << misc_fields - - // Render the JSON template - def engine = new groovy.text.GStringTemplateEngine() - // Different JSON depending on the service provider - // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format - def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" - def hf = new File("$projectDir/assets/${json_path}") - def json_template = engine.createTemplate(hf).make(msg_fields) - def json_message = json_template.toString() - - // POST - def post = new URL(hook_url).openConnection(); - post.setRequestMethod("POST") - post.setDoOutput(true) - post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); - } - } - - // - // Dump pipeline parameters in a json file - // - public static void dump_parameters(workflow, params) { - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def filename = "params_${timestamp}.json" - def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = JsonOutput.toJson(params) - temp_pf.text = JsonOutput.prettyPrint(jsonStr) - - FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") - temp_pf.delete() - } - - // - // Print pipeline summary on completion - // - public static void summary(workflow, params, log) { - Map colors = logColours(params.monochrome_logs) - if (workflow.success) { - if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" - } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" - } - } - - // - // ANSII Colours used for terminal logging - // - public static Map logColours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - // - // Does what is says on the tin - // - public static String dashedLine(monochrome_logs) { - Map colors = logColours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - // - // nf-core logo - // - public static String logo(workflow, monochrome_logs) { - Map colors = logColours(monochrome_logs) - String workflow_version = NfcoreTemplate.version(workflow) - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/lib/Utils.groovy b/lib/Utils.groovy deleted file mode 100644 index 8d030f4e..00000000 --- a/lib/Utils.groovy +++ /dev/null @@ -1,47 +0,0 @@ -// -// This file holds several Groovy functions that could be useful for any Nextflow pipeline -// - -import org.yaml.snakeyaml.Yaml - -class Utils { - - // - // When running with -profile conda, warn if channels have not been set-up appropriately - // - public static void checkCondaChannels(log) { - Yaml parser = new Yaml() - def channels = [] - try { - def config = parser.load("conda config --show channels".execute().text) - channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return - } - - // Check that all channels are present - // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] - def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean - - // Check that they are in the right order - def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) - } - - if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - } - } -} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy deleted file mode 100755 index b8a1723f..00000000 --- a/lib/WorkflowMain.groovy +++ /dev/null @@ -1,77 +0,0 @@ -// -// This file holds several functions specific to the main.nf workflow in the nf-core/rnafusion pipeline -// - -import nextflow.Nextflow - -class WorkflowMain { - - // - // Citation string for pipeline - // - public static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - // TODO nf-core: Add Zenodo DOI for pipeline after first release - //"* The pipeline\n" + - //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" - } - - - // - // Validate parameters and print summary to screen - // - public static void initialise(workflow, params, log, args) { - - // Print workflow version and exit on --version - if (params.version) { - String workflow_version = NfcoreTemplate.version(workflow) - log.info "${workflow.manifest.name} ${workflow_version}" - System.exit(0) - } - - // Check that a -profile or Nextflow config has been provided to run the pipeline - NfcoreTemplate.checkConfigProvided(workflow, log) - // Check that the profile doesn't contain spaces and doesn't end with a trailing comma - checkProfile(workflow.profile, args, log) - - // Check that conda channels are set-up correctly - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - Utils.checkCondaChannels(log) - } - - // Check AWS batch settings - NfcoreTemplate.awsBatch(workflow, params) - - // Check input has been provided - if (!params.input) { - Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") - } - } - // - // Get attribute from genome config file e.g. fasta - // - public static Object getGenomeAttribute(params, attribute) { - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - return params.genomes[ params.genome ][ attribute ] - } - } - return null - } - - // - // Exit pipeline if --profile contains spaces - // - private static void checkProfile(profile, args, log) { - if (profile.endsWith(',')) { - Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - if (args[0]) { - log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - } -} diff --git a/lib/WorkflowRnafusion.groovy b/lib/WorkflowRnafusion.groovy deleted file mode 100755 index 1e289fdc..00000000 --- a/lib/WorkflowRnafusion.groovy +++ /dev/null @@ -1,122 +0,0 @@ -// -// This file holds several functions specific to the workflow/rnafusion.nf in the nf-core/rnafusion pipeline -// - -import nextflow.Nextflow -import groovy.text.SimpleTemplateEngine - -class WorkflowRnafusion { - - // - // Check and validate parameters - // - public static void initialise(params, log) { - - genomeExistsError(params, log) - - - if (!params.fasta) { - Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - } - } - - // - // Get workflow summary for MultiQC - // - public static String paramsSummaryMultiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

$group

\n" - summary_section += "
\n" - for (param in group_params.keySet()) { - summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" - } - summary_section += "
\n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } - - // - // Generate methods description for MultiQC - // - - public static String toolCitationText(params) { - - // TODO nf-core: Optionally add in-text citation tools to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def citation_text = [ - "Tools used in the workflow included:", - "FastQC (Andrews 2010),", - "MultiQC (Ewels et al. 2016)", - "." - ].join(' ').trim() - - return citation_text - } - - public static String toolBibliographyText(params) { - - // TODO Optionally add bibliographic entries to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " - ].join(' ').trim() - - return reference_text - } - - public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { - // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file - def meta = [:] - meta.workflow = run_workflow.toMap() - meta["manifest_map"] = run_workflow.manifest.toMap() - - // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " - - // Tool references - meta["tool_citations"] = "" - meta["tool_bibliography"] = "" - - // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - //meta["tool_bibliography"] = toolBibliographyText(params) - - - def methods_text = mqc_methods_yaml.text - - def engine = new SimpleTemplateEngine() - def description_html = engine.createTemplate(methods_text).make(meta) - - return description_html - } - - // - // Exit pipeline if incorrect --genome key provided - // - private static void genomeExistsError(params, log) { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + - " Currently, the available genome keys are:\n" + - " ${params.genomes.keySet().join(", ")}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - Nextflow.error(error_string) - } - } -} diff --git a/main.nf b/main.nf index 47c38e5e..91475247 100644 --- a/main.nf +++ b/main.nf @@ -13,66 +13,96 @@ nextflow.enable.dsl = 2 /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - GENOME PARAMETER VALUES + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// TODO nf-core: Remove this line if you don't need a FASTA file -// This is an example of how to use getGenomeAttribute() to fetch parameters -// from igenomes.config using `--genome` -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +include { RNAFUSION } from './workflows/rnafusion' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_rnafusion_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_rnafusion_pipeline' + +include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_rnafusion_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - VALIDATE & PRINT PARAMETER SUMMARY + GENOME PARAMETER VALUES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { validateParameters; paramsHelp } from 'plugin/nf-validation' - -// Print help message if needed -if (params.help) { - def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) - def citation = '\n' + WorkflowMain.citation(workflow) + '\n' - def String command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" - log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs) - System.exit(0) -} - -// Validate input parameters -if (params.validate_params) { - validateParameters() -} - -WorkflowMain.initialise(workflow, params, log, args) +// TODO nf-core: Remove this line if you don't need a FASTA file +// This is an example of how to use getGenomeAttribute() to fetch parameters +// from igenomes.config using `--genome` +params.fasta = getGenomeAttribute('fasta') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - NAMED WORKFLOW FOR PIPELINE + NAMED WORKFLOWS FOR PIPELINE ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { RNAFUSION } from './workflows/rnafusion' - // -// WORKFLOW: Run main nf-core/rnafusion analysis pipeline +// WORKFLOW: Run main analysis pipeline depending on type of input // workflow NFCORE_RNAFUSION { - RNAFUSION () -} + take: + samplesheet // channel: samplesheet read in from --input + + main: + + // + // WORKFLOW: Run pipeline + // + RNAFUSION ( + samplesheet + ) + + emit: + multiqc_report = RNAFUSION.out.multiqc_report // channel: /path/to/multiqc_report.html + +} /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - RUN ALL WORKFLOWS + RUN MAIN WORKFLOW ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// -// WORKFLOW: Execute a single named workflow for the pipeline -// See: https://github.com/nf-core/rnaseq/issues/619 -// workflow { - NFCORE_RNAFUSION () + + main: + + // + // SUBWORKFLOW: Run initialisation tasks + // + PIPELINE_INITIALISATION ( + params.version, + params.help, + params.validate_params, + params.monochrome_logs, + args, + params.outdir, + params.input + ) + + // + // WORKFLOW: Run main workflow + // + NFCORE_RNAFUSION ( + PIPELINE_INITIALISATION.out.samplesheet + ) + + // + // SUBWORKFLOW: Run completion tasks + // + PIPELINE_COMPLETION ( + params.email, + params.email_on_fail, + params.plaintext_email, + params.outdir, + params.monochrome_logs, + params.hook_url, + NFCORE_RNAFUSION.out.multiqc_report + ) } /* diff --git a/modules.json b/modules.json index a7097920..6d161c68 100644 --- a/modules.json +++ b/modules.json @@ -5,22 +5,36 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] - }, "fastqc": { "branch": "master", - "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", + "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", + "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290", "installed_by": ["modules"] } } + }, + "subworkflows": { + "nf-core": { + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "installed_by": ["subworkflows"] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + } + } } } } diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf deleted file mode 100644 index 9226705d..00000000 --- a/modules/local/samplesheet_check.nf +++ /dev/null @@ -1,31 +0,0 @@ -process SAMPLESHEET_CHECK { - tag "$samplesheet" - label 'process_single' - - conda "conda-forge::python=3.8.3" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'biocontainers/python:3.8.3' }" - - input: - path samplesheet - - output: - path '*.csv' , emit: csv - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: // This script is bundled with the pipeline, in nf-core/rnafusion/bin/ - """ - check_samplesheet.py \\ - $samplesheet \\ - samplesheet.valid.csv - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | sed 's/Python //g') - END_VERSIONS - """ -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml deleted file mode 100644 index 9b3272bc..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: custom_dumpsoftwareversions -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf deleted file mode 100644 index f2187611..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ /dev/null @@ -1,24 +0,0 @@ -process CUSTOM_DUMPSOFTWAREVERSIONS { - label 'process_single' - - // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" - - input: - path versions - - output: - path "software_versions.yml" , emit: yml - path "software_versions_mqc.yml", emit: mqc_yml - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - template 'dumpsoftwareversions.py' -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml deleted file mode 100644 index 5f15a5fd..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ /dev/null @@ -1,37 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: custom_dumpsoftwareversions -description: Custom module used to dump software versions within the nf-core pipeline template -keywords: - - custom - - dump - - version -tools: - - custom: - description: Custom module used to dump software versions within the nf-core pipeline template - homepage: https://github.com/nf-core/tools - documentation: https://github.com/nf-core/tools - licence: ["MIT"] -input: - - versions: - type: file - description: YML file containing software versions - pattern: "*.yml" -output: - - yml: - type: file - description: Standard YML file containing software versions - pattern: "software_versions.yml" - - mqc_yml: - type: file - description: MultiQC custom content YML file containing software versions - pattern: "software_versions_mqc.yml" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" -maintainers: - - "@drpatelh" - - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py deleted file mode 100755 index e55b8d43..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ /dev/null @@ -1,102 +0,0 @@ -#!/usr/bin/env python - - -"""Provide functions to merge multiple versions.yml files.""" - - -import platform -from textwrap import dedent - -import yaml - - -def _make_versions_html(versions): - """Generate a tabular HTML output of all versions for MultiQC.""" - html = [ - dedent( - """\\ - -

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Download and build references - - specified with `--build_references` parameter - - required only once before running the pipeline - - **Important**: has to be run with each new release + +- specified with `--build_references` parameter +- required only once before running the pipeline +- **Important**: has to be run with each new release + 2. Detecting fusions - - Supported tools: `Arriba`, `FusionCatcher`, `STAR-Fusion`, and `StringTie` - - QC: `Fastqc`, `MultiQC`, and `Picard CollectInsertSize`, `Picard CollectWgsMetrics`, `Picard Markduplicates` - - Fusions visualization: `Arriba`, `fusion-report`, `FusionInspector`, and `vcf_collect` + +- Supported tools: `Arriba`, `FusionCatcher`, `STAR-Fusion`, and `StringTie` +- QC: `Fastqc`, `MultiQC`, and `Picard CollectInsertSize`, `Picard CollectWgsMetrics`, `Picard Markduplicates` +- Fusions visualization: `Arriba`, `fusion-report`, `FusionInspector`, and `vcf_collect` ## Download and build references From bc0e366a2483584342483c6175d7fb673c9bb952 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 2 Feb 2024 13:26:52 +0100 Subject: [PATCH 20/64] update logos from TEMPLATES --- assets/nf-core-rnafusion_logo_light.png | Bin 75832 -> 75831 bytes docs/images/nf-core-rnafusion_logo_dark.png | Bin 27732 -> 27749 bytes 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    Process Name \\", + " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    - - - - - - - - """ - ) - ] - for process, tmp_versions in sorted(versions.items()): - html.append("") - for i, (tool, version) in enumerate(sorted(tmp_versions.items())): - html.append( - dedent( - f"""\\ - - - - - - """ - ) - ) - html.append("") - html.append("
    Process Name Software Version
    {process if (i == 0) else ''}{tool}{version}
    ") - return "\\n".join(html) - - -def main(): - """Load all version files and generate merged output.""" - versions_this_module = {} - versions_this_module["${task.process}"] = { - "python": platform.python_version(), - "yaml": yaml.__version__, - } - - with open("$versions") as f: - versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module - - # aggregate versions by the module name (derived from fully-qualified process name) - versions_by_module = {} - for process, process_versions in versions_by_process.items(): - module = process.split(":")[-1] - try: - if versions_by_module[module] != process_versions: - raise AssertionError( - "We assume that software versions are the same between all modules. " - "If you see this error-message it means you discovered an edge-case " - "and should open an issue in nf-core/tools. " - ) - except KeyError: - versions_by_module[module] = process_versions - - versions_by_module["Workflow"] = { - "Nextflow": "$workflow.nextflow.version", - "$workflow.manifest.name": "$workflow.manifest.version", - } - - versions_mqc = { - "id": "software_versions", - "section_name": "${workflow.manifest.name} Software Versions", - "section_href": "https://github.com/${workflow.manifest.name}", - "plot_type": "html", - "description": "are collected at run time from the software output.", - "data": _make_versions_html(versions_by_module), - } - - with open("software_versions.yml", "w") as f: - yaml.dump(versions_by_module, f, default_flow_style=False) - with open("software_versions_mqc.yml", "w") as f: - yaml.dump(versions_mqc, f, default_flow_style=False) - - with open("versions.yml", "w") as f: - yaml.dump(versions_this_module, f, default_flow_style=False) - - -if __name__ == "__main__": - main() diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test deleted file mode 100644 index b1e1630b..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ /dev/null @@ -1,43 +0,0 @@ -nextflow_process { - - name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" - script "../main.nf" - process "CUSTOM_DUMPSOFTWAREVERSIONS" - tag "modules" - tag "modules_nfcore" - tag "custom" - tag "dumpsoftwareversions" - tag "custom/dumpsoftwareversions" - - test("Should run without failures") { - when { - process { - """ - def tool1_version = ''' - TOOL1: - tool1: 0.11.9 - '''.stripIndent() - - def tool2_version = ''' - TOOL2: - tool2: 1.9 - '''.stripIndent() - - input[0] = Channel.of(tool1_version, tool2_version).collectFile() - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - process.out.versions, - file(process.out.mqc_yml[0]).readLines()[0..10], - file(process.out.yml[0]).readLines()[0..7] - ).match() - } - ) - } - } -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap deleted file mode 100644 index 5f59a936..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ /dev/null @@ -1,33 +0,0 @@ -{ - "Should run without failures": { - "content": [ - [ - "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" - ], - [ - "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", - " \\n\\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n \\n \\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n\\n\\n \\n\\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\" - ], - [ - "CUSTOM_DUMPSOFTWAREVERSIONS:", - " python: 3.11.7", - " yaml: 5.4.1", - "TOOL1:", - " tool1: 0.11.9", - "TOOL2:", - " tool2: '1.9'", - "Workflow:" - ] - ], - "timestamp": "2024-01-09T23:01:18.710682" - } -} \ No newline at end of file diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml deleted file mode 100644 index 405aa24a..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -custom/dumpsoftwareversions: - - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 1f21c664..70edae4d 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -33,7 +33,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_single") } ) } } @@ -63,7 +63,7 @@ nextflow_process { { assert path(process.out.html[0][1][0]).text.contains("") }, { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_paired") } ) } } @@ -89,7 +89,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } ) } } @@ -115,7 +115,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_bam") } ) } } @@ -153,7 +153,7 @@ nextflow_process { { assert path(process.out.html[0][1][2]).text.contains("") }, { assert path(process.out.html[0][1][3]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } ) } } @@ -179,7 +179,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } ) } } @@ -204,7 +204,7 @@ nextflow_process { { assert process.success }, { assert snapshot(process.out.html.collect { file(it[1]).getName() } + process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match() } + process.out.versions ).match("fastqc_stub") } ) } } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 5d624bb8..86f7c311 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,5 +1,17 @@ { - "sarscov2 single-end [fastq] - stub": { + "fastqc_versions_interleaved": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:07.293713" + }, + "fastqc_stub": { "content": [ [ "test.html", @@ -7,14 +19,70 @@ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:40:57.254299" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:31:01.425198" + }, + "fastqc_versions_multiple": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:55.797907" + }, + "fastqc_versions_bam": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:26.795862" + }, + "fastqc_versions_single": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:27.043675" + }, + "fastqc_versions_paired": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:47.584191" }, - "versions": { + "fastqc_versions_custom_prefix": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:36:50.033627" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:41:14.576531" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 7625b752..2212096a 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.19 + - bioconda::multiqc=1.20 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1b9f7c43..354f4430 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : + 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index d0438eda..f1c4242e 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -3,6 +3,7 @@ nextflow_process { name "Test Process MULTIQC" script "../main.nf" process "MULTIQC" + tag "modules" tag "modules_nfcore" tag "multiqc" @@ -12,7 +13,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -25,7 +26,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_single") } ) } @@ -36,7 +37,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) input[2] = [] input[3] = [] @@ -49,7 +50,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_config") } ) } } @@ -61,7 +62,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -75,7 +76,7 @@ nextflow_process { { assert snapshot(process.out.report.collect { file(it).getName() } + process.out.data.collect { file(it).getName() } + process.out.plots.collect { file(it).getName() } + - process.out.versions ).match() } + process.out.versions ).match("multiqc_stub") } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index d37e7304..c204b488 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -1,21 +1,41 @@ { - "versions": { + "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" ] ], - "timestamp": "2024-01-09T23:02:49.911994" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:28:51.744211298" }, - "sarscov2 single-end [fastqc] - stub": { + "multiqc_stub": { "content": [ [ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" ] ], - "timestamp": "2024-01-09T23:03:14.524346" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:29:28.847433492" + }, + "multiqc_versions_config": { + "content": [ + [ + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:29:13.223621555" } } \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index b52cb3ab..fc563542 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,9 +16,7 @@ params { genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false - - - // MultiQC options + fasta = null// MultiQC options multiqc_config = null multiqc_title = null multiqc_logo = null @@ -43,7 +41,6 @@ params { custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null - // Max resource options // Defaults only, expecting to be overwritten diff --git a/nextflow_schema.json b/nextflow_schema.json index 3acf2be1..025bd65d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -16,6 +16,7 @@ "type": "string", "format": "file-path", "exists": true, + "schema": "assets/schema_input.json", "mimetype": "text/csv", "pattern": "^\\S+\\.csv$", "description": "Path to comma-separated file containing information about the samples in the experiment.", diff --git a/pyproject.toml b/pyproject.toml index 7d08e1c8..56110621 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -3,11 +3,13 @@ [tool.ruff] line-length = 120 target-version = "py38" -select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] cache-dir = "~/.cache/ruff" -[tool.ruff.isort] +[tool.ruff.lint] +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] + +[tool.ruff.lint.isort] known-first-party = ["nf_core"] -[tool.ruff.per-file-ignores] +[tool.ruff.lint.per-file-ignores] "__init__.py" = ["E402", "F401"] diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf deleted file mode 100644 index 0aecf87f..00000000 --- a/subworkflows/local/input_check.nf +++ /dev/null @@ -1,44 +0,0 @@ -// -// Check input samplesheet and get read channels -// - -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' - -workflow INPUT_CHECK { - take: - samplesheet // file: /path/to/samplesheet.csv - - main: - SAMPLESHEET_CHECK ( samplesheet ) - .csv - .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channel(it) } - .set { reads } - - emit: - reads // channel: [ val(meta), [ reads ] ] - versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channel(LinkedHashMap row) { - // create meta map - def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() - - // add path(s) of the fastq file(s) to the meta map - def fastq_meta = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - fastq_meta = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" - } - fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return fastq_meta -} diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf new file mode 100644 index 00000000..dc90ad90 --- /dev/null +++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf @@ -0,0 +1,247 @@ +// +// Subworkflow with functionality specific to the nf-core/pipeline pipeline +// + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { fromSamplesheet } from 'plugin/nf-validation' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' +include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' +include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' +include { nfCoreLogo } from '../../nf-core/utils_nfcore_pipeline' +include { imNotification } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' +include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' + +/* +======================================================================================== + SUBWORKFLOW TO INITIALISE PIPELINE +======================================================================================== +*/ + +workflow PIPELINE_INITIALISATION { + + take: + version // boolean: Display version and exit + help // boolean: Display help text + validate_params // boolean: Boolean whether to validate parameters against the schema at runtime + monochrome_logs // boolean: Do not use coloured log outputs + nextflow_cli_args // array: List of positional nextflow CLI args + outdir // string: The output directory where the results will be saved + input // string: Path to input samplesheet + + main: + + ch_versions = Channel.empty() + + // + // Print version and exit if required and dump pipeline parameters to JSON file + // + UTILS_NEXTFLOW_PIPELINE ( + version, + true, + outdir, + workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 + ) + + // + // Validate parameters and generate parameter summary to stdout + // + pre_help_text = nfCoreLogo(monochrome_logs) + post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) + def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input samplesheet.csv --outdir " + UTILS_NFVALIDATION_PLUGIN ( + help, + workflow_command, + pre_help_text, + post_help_text, + validate_params, + "nextflow_schema.json" + ) + + // + // Check config provided to the pipeline + // + UTILS_NFCORE_PIPELINE ( + nextflow_cli_args + ) + // + // Custom validation for pipeline parameters + // + validateInputParameters() + + // + // Create channel from input file provided through params.input + // + Channel + .fromSamplesheet("input") + .map { + meta, fastq_1, fastq_2 -> + if (!fastq_2) { + return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] + } else { + return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] + } + } + .groupTuple() + .map { + validateInputSamplesheet(it) + } + .map { + meta, fastqs -> + return [ meta, fastqs.flatten() ] + } + .set { ch_samplesheet } + + emit: + samplesheet = ch_samplesheet + versions = ch_versions +} + +/* +======================================================================================== + SUBWORKFLOW FOR PIPELINE COMPLETION +======================================================================================== +*/ + +workflow PIPELINE_COMPLETION { + + take: + email // string: email address + email_on_fail // string: email address sent on pipeline failure + plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published + monochrome_logs // boolean: Disable ANSI colour codes in log output + hook_url // string: hook URL for notifications + multiqc_report // string: Path to MultiQC report + + main: + + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + + // + // Completion email and summary + // + workflow.onComplete { + if (email || email_on_fail) { + completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + } + + completionSummary(monochrome_logs) + + if (hook_url) { + imNotification(summary_params, hook_url) + } + } +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ +// +// Check and validate pipeline parameters +// +def validateInputParameters() { + genomeExistsError() +}// +// Validate channels from input samplesheet +// +def validateInputSamplesheet(input) { + def (metas, fastqs) = input[1..2] + + // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end + def endedness_ok = metas.collect{ it.single_end }.unique().size == 1 + if (!endedness_ok) { + error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") + } + + return [ metas[0], fastqs ] +} +// +// Get attribute from genome config file e.g. fasta +// +def getGenomeAttribute(attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null +} + +// +// Exit pipeline if incorrect --genome key provided +// +def genomeExistsError() { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + error(error_string) + } +}// +// Generate methods description for MultiQC +// +def toolCitationText() { + // TODO nf-core: Optionally add in-text citation tools to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def citation_text = [ + "Tools used in the workflow included:", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text +} + +def toolBibliographyText() { + // TODO nf-core: Optionally add bibliographic entries to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def reference_text = [ + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text +} + +def methodsDescriptionText(mqc_methods_yaml) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = workflow.toMap() + meta["manifest_map"] = workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = "" + meta["tool_bibliography"] = "" + + // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! + // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + // meta["tool_bibliography"] = toolBibliographyText() + + + def methods_text = mqc_methods_yaml.text + + def engine = new groovy.text.SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html.toString() +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf new file mode 100644 index 00000000..ac31f28f --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -0,0 +1,126 @@ +// +// Subworkflow with functionality that may be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NEXTFLOW_PIPELINE { + + take: + print_version // boolean: print version + dump_parameters // boolean: dump parameters + outdir // path: base directory used to publish pipeline results + check_conda_channels // boolean: check conda channels + + main: + + // + // Print workflow version and exit on --version + // + if (print_version) { + log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + System.exit(0) + } + + // + // Dump pipeline parameters to a JSON file + // + if (dump_parameters && outdir) { + dumpParametersToJSON(outdir) + } + + // + // When running with Conda, warn if channels have not been set-up appropriately + // + if (check_conda_channels) { + checkCondaChannels() + } + + emit: + dummy_emit = true +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Generate version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Dump pipeline parameters to a JSON file +// +def dumpParametersToJSON(outdir) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() +} + +// +// When running with -profile conda, warn if channels have not been set-up appropriately +// +def checkCondaChannels() { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml new file mode 100644 index 00000000..e5c3a0a8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml @@ -0,0 +1,38 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NEXTFLOW_PIPELINE" +description: Subworkflow with functionality that may be useful for any Nextflow pipeline +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - print_version: + type: boolean + description: | + Print the version of the pipeline and exit + - dump_parameters: + type: boolean + description: | + Dump the parameters of the pipeline to a JSON file + - output_directory: + type: directory + description: Path to output dir to write JSON file to. + pattern: "results/" + - check_conda_channel: + type: boolean + description: | + Check if the conda channel priority is correct. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" + - "@drpatelh" +maintainers: + - "@adamrtalbot" + - "@drpatelh" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..8ed4310c --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -0,0 +1,54 @@ + +nextflow_function { + + name "Test Functions" + script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Test Function getWorkflowVersion") { + + function "getWorkflowVersion" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dumpParametersToJSON") { + + function "dumpParametersToJSON" + + when { + function { + """ + // define inputs of the function here. Example: + input[0] = "$outputDir" + """.stripIndent() + } + } + + then { + assertAll( + { assert function.success } + ) + } + } + + test("Test Function checkCondaChannels") { + + function "checkCondaChannels" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..db2030f8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,12 @@ +{ + "Test Function getWorkflowVersion": { + "content": [ + "v9.9.9" + ], + "timestamp": "2024-01-19T11:32:36.031083" + }, + "Test Function checkCondaChannels": { + "content": null, + "timestamp": "2024-01-19T11:32:50.456" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..f7c54bc6 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,123 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NEXTFLOW_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + workflow "UTILS_NEXTFLOW_PIPELINE" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Should run no inputs") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = false + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should print version") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = true + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.stdout.contains("nextflow_workflow v9.9.9") } + ) + } + } + + test("Should dump params") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = params.outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should not create params JSON if no output directory") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = null + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config new file mode 100644 index 00000000..53574ffe --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml new file mode 100644 index 00000000..f8476112 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nextflow_pipeline: + - subworkflows/nf-core/utils_nextflow_pipeline/** diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf new file mode 100644 index 00000000..a8b55d6f --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -0,0 +1,440 @@ +// +// Subworkflow with utility functions specific to the nf-core pipeline template +// + +import org.yaml.snakeyaml.Yaml +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFCORE_PIPELINE { + + take: + nextflow_cli_args + + main: + valid_config = checkConfigProvided() + checkProfileProvided(nextflow_cli_args) + + emit: + valid_config +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Warn if a -profile or Nextflow config has not been provided to run the pipeline +// +def checkConfigProvided() { + valid_config = true + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + valid_config = false + } + return valid_config +} + +// +// Exit pipeline if --profile contains spaces +// +def checkProfileProvided(nextflow_cli_args) { + if (workflow.profile.endsWith(',')) { + error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } + if (nextflow_cli_args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } +} + +// +// Citation string for pipeline +// +def workflowCitation() { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + " ${workflow.manifest.doi}\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" +} + +// +// Generate workflow version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Get software versions for pipeline +// +def processVersionsFromYAML(yaml_file) { + Yaml yaml = new Yaml() + versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + return yaml.dumpAsMap(versions).trim() +} + +// +// Get workflow version for pipeline +// +def workflowVersionToYAML() { + return """ + Workflow: + $workflow.manifest.name: ${getWorkflowVersion()} + Nextflow: $workflow.nextflow.version + """.stripIndent().trim() +} + +// +// Get channel of software versions used in pipeline in YAML format +// +def softwareVersionsToYAML(ch_versions) { + return ch_versions + .unique() + .map { processVersionsFromYAML(it) } + .unique() + .mix(Channel.of(workflowVersionToYAML())) +} + +// +// Get workflow summary for MultiQC +// +def paramsSummaryMultiqc(summary_params) { + def summary_section = '' + for (group in summary_params.keySet()) { + def group_params = summary_params.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + + return yaml_file_text +} + +// +// nf-core logo +// +def nfCoreLogo(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) +} + +// +// Return dashed line +// +def dashedLine(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" +} + +// +// ANSII colours used for terminal logging +// +def logColours(monochrome_logs=true) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes +} + +// +// Attach the multiqc report to email +// +def attachMultiqcReport(multiqc_report) { + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + return mqc_report +} + +// +// Construct and send completion email +// +def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = getWorkflowVersion() + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = attachMultiqcReport(multiqc_report) + + // Check if we are only sending emails on failure + def email_address = email + if (!email && email_on_fail && !workflow.success) { + email_address = email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("${workflow.projectDir}/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("${workflow.projectDir}/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(monochrome_logs) + if (email_address) { + try { + if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() +} + +// +// Print pipeline summary on completion +// +def completionSummary(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } +} + +// +// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack +// +def imNotification(summary_params, hook_url) { + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = getWorkflowVersion() + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("${workflow.projectDir}/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml new file mode 100644 index 00000000..d08d2434 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml @@ -0,0 +1,24 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFCORE_PIPELINE" +description: Subworkflow with utility functions specific to the nf-core pipeline template +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - nextflow_cli_args: + type: list + description: | + Nextflow CLI positional arguments +output: + - success: + type: boolean + description: | + Dummy output to indicate success +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..1dc317f8 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test @@ -0,0 +1,134 @@ + +nextflow_function { + + name "Test Functions" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Test Function checkConfigProvided") { + + function "checkConfigProvided" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function checkProfileProvided") { + + function "checkProfileProvided" + + when { + function { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function workflowCitation") { + + function "workflowCitation" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function nfCoreLogo") { + + function "nfCoreLogo" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dashedLine") { + + function "dashedLine" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function without logColours") { + + function "logColours" + + when { + function { + """ + input[0] = true + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function with logColours") { + function "logColours" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..10f948e6 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,138 @@ +{ + "Test Function checkProfileProvided": { + "content": null, + "timestamp": "2024-02-09T15:43:55.145717" + }, + "Test Function checkConfigProvided": { + "content": [ + true + ], + "timestamp": "2024-01-19T11:34:13.548431224" + }, + "Test Function nfCoreLogo": { + "content": [ + "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" + ], + "timestamp": "2024-01-19T11:34:38.840454873" + }, + "Test Function workflowCitation": { + "content": [ + "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" + ], + "timestamp": "2024-01-19T11:34:22.24352016" + }, + "Test Function without logColours": { + "content": [ + { + "reset": "", + "bold": "", + "dim": "", + "underlined": "", + "blink": "", + "reverse": "", + "hidden": "", + "black": "", + "red": "", + "green": "", + "yellow": "", + "blue": "", + "purple": "", + "cyan": "", + "white": "", + "bblack": "", + "bred": "", + "bgreen": "", + "byellow": "", + "bblue": "", + "bpurple": "", + "bcyan": "", + "bwhite": "", + "ublack": "", + "ured": "", + "ugreen": "", + "uyellow": "", + "ublue": "", + "upurple": "", + "ucyan": "", + "uwhite": "", + "iblack": "", + "ired": "", + "igreen": "", + "iyellow": "", + "iblue": "", + "ipurple": "", + "icyan": "", + "iwhite": "", + "biblack": "", + "bired": "", + "bigreen": "", + "biyellow": "", + "biblue": "", + "bipurple": "", + "bicyan": "", + "biwhite": "" + } + ], + "timestamp": "2024-01-19T11:35:04.418416984" + }, + "Test Function dashedLine": { + "content": [ + "-\u001b[2m----------------------------------------------------\u001b[0m-" + ], + "timestamp": "2024-01-19T11:34:55.420000755" + }, + "Test Function with logColours": { + "content": [ + { + "reset": "\u001b[0m", + "bold": "\u001b[1m", + "dim": "\u001b[2m", + "underlined": "\u001b[4m", + "blink": "\u001b[5m", + "reverse": "\u001b[7m", + "hidden": "\u001b[8m", + "black": "\u001b[0;30m", + "red": "\u001b[0;31m", + "green": "\u001b[0;32m", + "yellow": "\u001b[0;33m", + "blue": "\u001b[0;34m", + "purple": "\u001b[0;35m", + "cyan": "\u001b[0;36m", + "white": "\u001b[0;37m", + "bblack": "\u001b[1;30m", + "bred": "\u001b[1;31m", + "bgreen": "\u001b[1;32m", + "byellow": "\u001b[1;33m", + "bblue": "\u001b[1;34m", + "bpurple": "\u001b[1;35m", + "bcyan": "\u001b[1;36m", + "bwhite": "\u001b[1;37m", + "ublack": "\u001b[4;30m", + "ured": "\u001b[4;31m", + "ugreen": "\u001b[4;32m", + "uyellow": "\u001b[4;33m", + "ublue": "\u001b[4;34m", + "upurple": "\u001b[4;35m", + "ucyan": "\u001b[4;36m", + "uwhite": "\u001b[4;37m", + "iblack": "\u001b[0;90m", + "ired": "\u001b[0;91m", + "igreen": "\u001b[0;92m", + "iyellow": "\u001b[0;93m", + "iblue": "\u001b[0;94m", + "ipurple": "\u001b[0;95m", + "icyan": "\u001b[0;96m", + "iwhite": "\u001b[0;97m", + "biblack": "\u001b[1;90m", + "bired": "\u001b[1;91m", + "bigreen": "\u001b[1;92m", + "biyellow": "\u001b[1;93m", + "biblue": "\u001b[1;94m", + "bipurple": "\u001b[1;95m", + "bicyan": "\u001b[1;96m", + "biwhite": "\u001b[1;97m" + } + ], + "timestamp": "2024-01-19T11:35:13.436366565" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..8940d32d --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,29 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFCORE_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + workflow "UTILS_NFCORE_PIPELINE" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Should run without failures") { + + when { + workflow { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot(workflow.out).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap new file mode 100644 index 00000000..d07ce54c --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -0,0 +1,15 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + true + ], + "valid_config": [ + true + ] + } + ], + "timestamp": "2024-01-19T11:35:22.538940073" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config new file mode 100644 index 00000000..d0a926bf --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml new file mode 100644 index 00000000..ac8523c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfcore_pipeline: + - subworkflows/nf-core/utils_nfcore_pipeline/** diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf new file mode 100644 index 00000000..2585b65d --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf @@ -0,0 +1,62 @@ +// +// Subworkflow that uses the nf-validation plugin to render help text and parameter summary +// + +/* +======================================================================================== + IMPORT NF-VALIDATION PLUGIN +======================================================================================== +*/ + +include { paramsHelp } from 'plugin/nf-validation' +include { paramsSummaryLog } from 'plugin/nf-validation' +include { validateParameters } from 'plugin/nf-validation' + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFVALIDATION_PLUGIN { + + take: + print_help // boolean: print help + workflow_command // string: default commmand used to run pipeline + pre_help_text // string: string to be printed before help text and summary log + post_help_text // string: string to be printed after help text and summary log + validate_params // boolean: validate parameters + schema_filename // path: JSON schema file, null to use default value + + main: + + log.debug "Using schema file: ${schema_filename}" + + // Default values for strings + pre_help_text = pre_help_text ?: '' + post_help_text = post_help_text ?: '' + workflow_command = workflow_command ?: '' + + // + // Print help message if needed + // + if (print_help) { + log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text + System.exit(0) + } + + // + // Print parameter summary to stdout + // + log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text + + // + // Validate parameters relative to the parameter JSON schema + // + if (validate_params){ + validateParameters(parameters_schema: schema_filename) + } + + emit: + dummy_emit = true +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml new file mode 100644 index 00000000..3d4a6b04 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml @@ -0,0 +1,44 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFVALIDATION_PLUGIN" +description: Use nf-validation to initiate and validate a pipeline +keywords: + - utility + - pipeline + - initialise + - validation +components: [] +input: + - print_help: + type: boolean + description: | + Print help message and exit + - workflow_command: + type: string + description: | + The command to run the workflow e.g. "nextflow run main.nf" + - pre_help_text: + type: string + description: | + Text to print before the help message + - post_help_text: + type: string + description: | + Text to print after the help message + - validate_params: + type: boolean + description: | + Validate the parameters and error if invalid. + - schema_filename: + type: string + description: | + The filename of the schema to validate against. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test new file mode 100644 index 00000000..517ee54e --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -0,0 +1,200 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFVALIDATION_PLUGIN" + script "../main.nf" + workflow "UTILS_NFVALIDATION_PLUGIN" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "plugin/nf-validation" + tag "'plugin/nf-validation'" + tag "utils_nfvalidation_plugin" + tag "subworkflows/utils_nfvalidation_plugin" + + test("Should run nothing") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should run help") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with command") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with extra text") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = "pre-help-text" + post_help_text = "post-help-text" + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('pre-help-text') } }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } }, + { assert workflow.stdout.any { it.contains('post-help-text') } } + ) + } + } + + test("Should validate params") { + + when { + + params { + monochrome_logs = true + test_data = '' + outdir = 1 + } + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = true + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json new file mode 100644 index 00000000..7626c1c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json @@ -0,0 +1,96 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", + "title": ". pipeline parameters", + "description": "", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["outdir"], + "properties": { + "validate_params": { + "type": "boolean", + "description": "Validate parameters?", + "default": true, + "hidden": true + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "test_data_base": { + "type": "string", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules", + "description": "Base for test data directory", + "hidden": true + }, + "test_data": { + "type": "string", + "description": "Fake test data param", + "hidden": true + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "logo": { + "type": "boolean", + "default": true, + "description": "Display nf-core logo in console output.", + "fa_icon": "fas fa-image", + "hidden": true + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Pull Singularity container from Docker?", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Use monochrome_logs", + "hidden": true + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml new file mode 100644 index 00000000..60b1cfff --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfvalidation_plugin: + - subworkflows/nf-core/utils_nfvalidation_plugin/** diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf index 027a8b38..62c2cf15 100644 --- a/workflows/rnafusion.nf +++ b/workflows/rnafusion.nf @@ -1,54 +1,15 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - PRINT PARAMS SUMMARY + IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' - -def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) -def citation = '\n' + WorkflowMain.citation(workflow) + '\n' -def summary_params = paramsSummaryMap(workflow) - -// Print parameter summary log to screen -log.info logo + paramsSummaryLog(workflow) + citation - -WorkflowRnafusion.initialise(params, log) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CONFIG FILES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT LOCAL MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// -include { INPUT_CHECK } from '../subworkflows/local/input_check' - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT NF-CORE MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// MODULE: Installed directly from nf-core/modules -// -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' +include { FASTQC } from '../modules/nf-core/fastqc/main' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_rnafusion_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -56,50 +17,45 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// Info required for completion email and summary -def multiqc_report = [] - workflow RNAFUSION { - ch_versions = Channel.empty() + take: + ch_samplesheet // channel: samplesheet read in from --input - // - // SUBWORKFLOW: Read in samplesheet, validate and stage input files - // - INPUT_CHECK ( - file(params.input) - ) - ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) - // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") - // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ - // ! There is currently no tooling to help you write a sample sheet schema + main: + + ch_versions = Channel.empty() + ch_multiqc_files = Channel.empty() // // MODULE: Run FastQC // FASTQC ( - INPUT_CHECK.out.reads + ch_samplesheet ) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique().collectFile(name: 'collated_versions.yml') - ) + // + // Collate and save software versions + // + softwareVersionsToYAML(ch_versions) + .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) + .set { ch_collated_versions } // // MODULE: MultiQC // - workflow_summary = WorkflowRnafusion.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - methods_description = WorkflowRnafusion.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) - ch_methods_description = Channel.value(methods_description) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) MULTIQC ( ch_multiqc_files.collect(), @@ -107,31 +63,10 @@ workflow RNAFUSION { ch_multiqc_custom_config.toList(), ch_multiqc_logo.toList() ) - multiqc_report = MULTIQC.out.report.toList() -} - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - COMPLETION EMAIL AND SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -workflow.onComplete { - if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) - } - NfcoreTemplate.dump_parameters(workflow, params) - NfcoreTemplate.summary(workflow, params, log) - if (params.hook_url) { - NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log) - } -} -workflow.onError { - if (workflow.errorReport.contains("Process requirement exceeds available memory")) { - println("🛑 Default resources exceed availability 🛑 ") - println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") - } + emit: + multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html + versions = ch_versions // channel: [ path(versions.yml) ] } /* From 05ffaee8e4b8a363e6654d20695504e498830b6e Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 29 Feb 2024 16:11:57 +0000 Subject: [PATCH 23/64] Template update for nf-core/tools version 2.13.1 --- .devcontainer/devcontainer.json | 10 +---- .github/CONTRIBUTING.md | 14 ++++--- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/awsfulltest.yml | 4 +- .github/workflows/awstest.yml | 4 +- .github/workflows/ci.yml | 2 +- .github/workflows/download_pipeline.yml | 2 +- .github/workflows/linting.yml | 2 +- .github/workflows/release-announcements.yml | 2 +- .gitpod.yml | 6 +-- README.md | 3 +- modules.json | 8 ++-- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- .../nf-core/multiqc/tests/main.nf.test.snap | 12 +++--- .../utils_nfcore_rnafusion_pipeline/main.nf | 10 +++-- .../tests/main.function.nf.test | 2 +- .../tests/main.function.nf.test.snap | 12 +++++- .../tests/main.workflow.nf.test | 20 ++------- .../tests/nextflow.config | 2 +- .../tests/main.function.nf.test.snap | 42 +++++++++++++++---- .../tests/main.workflow.nf.test.snap | 6 ++- .../tests/main.nf.test | 2 +- 23 files changed, 99 insertions(+), 74 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4ecfbfe3..b290e090 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -10,15 +10,7 @@ "vscode": { // Set *default* container specific settings.json values on container create. "settings": { - "python.defaultInterpreterPath": "/opt/conda/bin/python", - "python.linting.enabled": true, - "python.linting.pylintEnabled": true, - "python.formatting.autopep8Path": "/opt/conda/bin/autopep8", - "python.formatting.yapfPath": "/opt/conda/bin/yapf", - "python.linting.flake8Path": "/opt/conda/bin/flake8", - "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", - "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" + "python.defaultInterpreterPath": "/opt/conda/bin/python" }, // Add the IDs of extensions you want installed when the container is created. diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index a0b73d5c..8e5d1fef 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,9 +9,8 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -:::info -If you need help using or modifying nf-core/rnafusion then the best place to ask is on the nf-core Slack [#rnafusion](https://nfcore.slack.com/channels/rnafusion) channel ([join our Slack here](https://nf-co.re/join/slack)). -::: +> [!NOTE] +> If you need help using or modifying nf-core/rnafusion then the best place to ask is on the nf-core Slack [#rnafusion](https://nfcore.slack.com/channels/rnafusion) channel ([join our Slack here](https://nf-co.re/join/slack)). ## Contribution workflow @@ -27,8 +26,11 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests -You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to -receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. +You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command: + +```bash +nf-test test --profile debug,test,docker --verbose +``` When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. @@ -90,7 +92,7 @@ Once there, use `nf-core schema build` to add to `nextflow_schema.json`. Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. -The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. +The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. ### Naming schemes diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index ed1d027b..8b85a0aa 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnaf - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index b46a4819..a4b6b4d3 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 + uses: seqeralabs/action-tower-launch@v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 104df35b..cf29b8fd 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,7 +12,7 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 + uses: seqeralabs/action-tower-launch@v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index bdbc1fcb..3390bf1a 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -31,7 +31,7 @@ jobs: uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 with: version: "${{ matrix.NXF_VER }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index f823210d..08622fd5 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -28,7 +28,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 748b4311..073e1876 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -35,7 +35,7 @@ jobs: uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index c3674af2..d468aeaa 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -12,7 +12,7 @@ jobs: - name: get topics and convert to hashtags id: get_topics run: | - curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' > $GITHUB_OUTPUT + curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT - uses: rzr/fediverse-action@master with: diff --git a/.gitpod.yml b/.gitpod.yml index 363d5b1d..105a1821 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -10,13 +10,11 @@ tasks: vscode: extensions: # based on nf-core.nf-core-extensionpack - - codezombiech.gitignore # Language support for .gitignore files - # - cssho.vscode-svgviewer # SVG viewer - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code - - eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar - mechatroner.rainbow-csv # Highlight columns in csv files in different colors - # - nextflow.nextflow # Nextflow syntax highlighting + # - nextflow.nextflow # Nextflow syntax highlighting - oderwat.indent-rainbow # Highlight indentation level - streetsidesoftware.code-spell-checker # Spelling checker for source code + - charliermarsh.ruff # Code linter Ruff diff --git a/README.md b/README.md index ca941ca5..805de70e 100644 --- a/README.md +++ b/README.md @@ -7,12 +7,13 @@ [![GitHub Actions CI Status](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/ci.yml) [![GitHub Actions Linting Status](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/rnafusion/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/rnafusion/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/rnafusion) +[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/rnafusion) [![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23rnafusion-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/rnafusion)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) diff --git a/modules.json b/modules.json index 6d161c68..5002e346 100644 --- a/modules.json +++ b/modules.json @@ -12,7 +12,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290", + "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", "installed_by": ["modules"] } } @@ -21,17 +21,17 @@ "nf-core": { "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] } } diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 2212096a..ca39fb67 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.20 + - bioconda::multiqc=1.21 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 354f4430..47ac352f 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : - 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : + 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index c204b488..bfebd802 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -2,14 +2,14 @@ "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:28:51.744211298" + "timestamp": "2024-02-29T08:48:55.657331" }, "multiqc_stub": { "content": [ @@ -17,25 +17,25 @@ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:29:28.847433492" + "timestamp": "2024-02-29T08:49:49.071937" }, "multiqc_versions_config": { "content": [ [ - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:29:13.223621555" + "timestamp": "2024-02-29T08:49:25.457567" } } \ No newline at end of file diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf index dc90ad90..c24a3741 100644 --- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf @@ -1,5 +1,5 @@ // -// Subworkflow with functionality specific to the nf-core/pipeline pipeline +// Subworkflow with functionality specific to the nf-core/rnafusion pipeline // /* @@ -152,7 +152,9 @@ workflow PIPELINE_COMPLETION { // def validateInputParameters() { genomeExistsError() -}// +} + +// // Validate channels from input samplesheet // def validateInputSamplesheet(input) { @@ -190,7 +192,9 @@ def genomeExistsError() { "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" error(error_string) } -}// +} + +// // Generate methods description for MultiQC // def toolCitationText() { diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test index 8ed4310c..68718e4f 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -51,4 +51,4 @@ nextflow_function { ) } } -} \ No newline at end of file +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap index db2030f8..e3f0baf4 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -3,10 +3,18 @@ "content": [ "v9.9.9" ], - "timestamp": "2024-01-19T11:32:36.031083" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:05.308243" }, "Test Function checkCondaChannels": { "content": null, - "timestamp": "2024-01-19T11:32:50.456" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:12.425833" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test index f7c54bc6..ca964ce8 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -11,9 +11,6 @@ nextflow_workflow { test("Should run no inputs") { when { - params { - outdir = "tests/results" - } workflow { """ print_version = false @@ -39,9 +36,6 @@ nextflow_workflow { test("Should print version") { when { - params { - outdir = "tests/results" - } workflow { """ print_version = true @@ -68,19 +62,16 @@ nextflow_workflow { test("Should dump params") { when { - params { - outdir = "$outputDir" - } workflow { """ print_version = false dump_parameters = true - outdir = params.outdir + outdir = 'results' check_conda_channels = false input[0] = false input[1] = true - input[2] = params.outdir + input[2] = outdir input[3] = false """ } @@ -96,19 +87,16 @@ nextflow_workflow { test("Should not create params JSON if no output directory") { when { - params { - outdir = "$outputDir" - } workflow { """ print_version = false dump_parameters = true - outdir = params.outdir + outdir = null check_conda_channels = false input[0] = false input[1] = true - input[2] = null + input[2] = outdir input[3] = false """ } diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config index 53574ffe..d0a926bf 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -6,4 +6,4 @@ manifest { nextflowVersion = '!>=23.04.0' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' -} \ No newline at end of file +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap index 10f948e6..1037232c 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -1,25 +1,41 @@ { "Test Function checkProfileProvided": { "content": null, - "timestamp": "2024-02-09T15:43:55.145717" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:03.360873" }, "Test Function checkConfigProvided": { "content": [ true ], - "timestamp": "2024-01-19T11:34:13.548431224" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:59.729647" }, "Test Function nfCoreLogo": { "content": [ "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" ], - "timestamp": "2024-01-19T11:34:38.840454873" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:10.562934" }, "Test Function workflowCitation": { "content": [ "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" ], - "timestamp": "2024-01-19T11:34:22.24352016" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:07.019761" }, "Test Function without logColours": { "content": [ @@ -73,13 +89,21 @@ "biwhite": "" } ], - "timestamp": "2024-01-19T11:35:04.418416984" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:17.969323" }, "Test Function dashedLine": { "content": [ "-\u001b[2m----------------------------------------------------\u001b[0m-" ], - "timestamp": "2024-01-19T11:34:55.420000755" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:14.366181" }, "Test Function with logColours": { "content": [ @@ -133,6 +157,10 @@ "biwhite": "\u001b[1;97m" } ], - "timestamp": "2024-01-19T11:35:13.436366565" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:21.714424" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap index d07ce54c..859d1030 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -10,6 +10,10 @@ ] } ], - "timestamp": "2024-01-19T11:35:22.538940073" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:25.726491" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test index 517ee54e..5784a33f 100644 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -197,4 +197,4 @@ nextflow_workflow { ) } } -} \ No newline at end of file +} From ec3e795c4604e25ecada43f2bb3671c989a4e08c Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 4 Mar 2024 09:34:38 +0100 Subject: [PATCH 24/64] update collectrnaseqmetrics memory requirement --- bin/check_samplesheet.py | 4 +- bin/vcf_collect.py | 60 ++++++++++++++----- .../local/picard/collectrnaseqmetrics/main.nf | 14 ++--- 3 files changed, 54 insertions(+), 24 deletions(-) diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 57cf8e6b..218dd802 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -123,7 +123,9 @@ def _validate_fastq_format(self, filename): def _validate_strandedness(self, row): """Assert that the strandedness given is one of unstranded/forward/reverse""" if row[self._strandedness] not in self.VALID_STRANDEDNESSES: - raise AssertionError(f"Strandedness must be one of {', '.join(self.VALID_STRANDEDNESSES)}") + raise AssertionError( + f"Strandedness must be one of {', '.join(self.VALID_STRANDEDNESSES)}" + ) def validate_unique_samples(self): """ diff --git a/bin/vcf_collect.py b/bin/vcf_collect.py index 7e988c1e..61283d3b 100755 --- a/bin/vcf_collect.py +++ b/bin/vcf_collect.py @@ -65,7 +65,9 @@ def vcf_collect( gtf_df = build_gtf_dataframe(gtf) all_df = df.merge(gtf_df, how="left", left_on="CDS_LEFT_ID", right_on="Transcript_id") - all_df[["PosA", "orig_start", "orig_end"]] = all_df[["PosA", "orig_start", "orig_end"]].fillna(0).astype(int) + all_df[["PosA", "orig_start", "orig_end"]] = ( + all_df[["PosA", "orig_start", "orig_end"]].fillna(0).astype(int) + ) all_df = all_df[ ((all_df["PosA"] >= all_df["orig_start"]) & (all_df["PosA"] <= all_df["orig_end"])) @@ -75,7 +77,9 @@ def vcf_collect( all_df.replace("", np.nan, inplace=True) all_df = all_df.drop_duplicates() - all_df[["exon_number", "transcript_version"]] = all_df[["exon_number", "transcript_version"]].replace(0, np.nan) + all_df[["exon_number", "transcript_version"]] = all_df[ + ["exon_number", "transcript_version"] + ].replace(0, np.nan) # Fill non-empty values within each group for 'exon_number' and 'transcript_version' all_df["exon_number"] = all_df.groupby("PosA")["exon_number"].transform( lambda x: x.fillna(method="ffill").fillna(method="bfill") @@ -116,8 +120,12 @@ def vcf_collect( ].drop_duplicates() all_df["CDS_RIGHT_ID"] = all_df["CDS_RIGHT_ID"].astype("str") all_df = all_df.merge(gtf_df, how="left", left_on="CDS_RIGHT_ID", right_on="Transcript_id") - all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].fillna(0) - all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].astype(int) + all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].fillna( + 0 + ) + all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].astype( + int + ) all_df = all_df[ ((all_df["PosB"] >= all_df["orig_start"]) & (all_df["PosB"] <= all_df["orig_end"])) | ((all_df["orig_start"] == 0) & (all_df["orig_end"] == 0)) @@ -126,7 +134,9 @@ def vcf_collect( all_df[["PosA", "PosB"]] = all_df[["PosA", "PosB"]].replace(0, np.nan) all_df = all_df.replace("", np.nan) - all_df[["exon_number", "transcript_version"]] = all_df[["exon_number", "transcript_version"]].replace(0, np.nan) + all_df[["exon_number", "transcript_version"]] = all_df[ + ["exon_number", "transcript_version"] + ].replace(0, np.nan) # Fill non-empty values within each group for 'exon_number' and 'transcript_version' all_df["exon_number"] = all_df.groupby("PosB")["exon_number"].transform( lambda x: x.fillna(method="ffill").fillna(method="bfill") @@ -212,7 +222,9 @@ def parse_args(argv=None): type=Path, help="HGNC database.", ) - parser.add_argument("--sample", metavar="SAMPLE", type=Path, help="Sample name.", default="Sample") + parser.add_argument( + "--sample", metavar="SAMPLE", type=Path, help="Sample name.", default="Sample" + ) parser.add_argument( "--out", metavar="OUT", @@ -280,7 +292,11 @@ def build_fusioninspector_dataframe(file: str) -> pd.DataFrame: df["annots"] = ( df["annots"] .apply(convert_to_list) - .apply(lambda x: ",".join(map(str, x)) if isinstance(x, list) else str(x) if pd.notna(x) else "") + .apply( + lambda x: ( + ",".join(map(str, x)) if isinstance(x, list) else str(x) if pd.notna(x) else "" + ) + ) ) else: for i in [ @@ -359,9 +375,9 @@ def read_build_fusionreport(fusionreport_file: str) -> pd.DataFrame: fusion_report["FOUND_DB"] = fusion_report["FOUND_DB"].apply(lambda x: ",".join(x)) fusion_report[["GeneA", "GeneB"]] = fusion_report["FUSION"].str.split("--", expand=True) - return fusion_report[["FUSION", "GeneA", "GeneB", "TOOLS_HITS", "SCORE", "FOUND_DB", "FOUND_IN"]].set_index( - ["FUSION"] - ) + return fusion_report[ + ["FUSION", "GeneA", "GeneB", "TOOLS_HITS", "SCORE", "FOUND_DB", "FOUND_IN"] + ].set_index(["FUSION"]) def read_fusionreport_csv(file: str) -> pd.DataFrame: @@ -370,7 +386,9 @@ def read_fusionreport_csv(file: str) -> pd.DataFrame: for column in columns_to_iterate: if column not in df.columns: df[column] = "" - df[["starfusion", "arriba", "fusioncatcher"]] = df[["starfusion", "arriba", "fusioncatcher"]].astype("str") + df[["starfusion", "arriba", "fusioncatcher"]] = df[ + ["starfusion", "arriba", "fusioncatcher"] + ].astype("str") for index, row in df.iterrows(): for column in columns_to_iterate: cell_value = row[column] @@ -398,7 +416,9 @@ def read_fusionreport_csv(file: str) -> pd.DataFrame: df[["GeneA", "GeneB"]] = df["Fusion"].str.split("--", expand=True) df = df.set_index("Fusion") df.to_csv("tmp.csv") - return df[["GeneA", "GeneB", "ChromosomeA", "PosA", "StrandA", "ChromosomeB", "PosB", "StrandB"]] + return df[ + ["GeneA", "GeneB", "ChromosomeA", "PosA", "StrandA", "ChromosomeB", "PosB", "StrandB"] + ] def column_manipulation(df: pd.DataFrame) -> pd.DataFrame: @@ -425,7 +445,9 @@ def column_manipulation(df: pd.DataFrame) -> pd.DataFrame: df["Left_exon_number"] = df["Left_exon_number"].fillna(0).astype(int).astype(str) df["Right_exon_number"] = df["Right_exon_number"].fillna(0).astype(int).astype(str) df["Left_transcript_version"] = df["Left_transcript_version"].fillna(0).astype(int).astype(str) - df["Right_transcript_version"] = df["Right_transcript_version"].fillna(0).astype(int).astype(str) + df["Right_transcript_version"] = ( + df["Right_transcript_version"].fillna(0).astype(int).astype(str) + ) df["PosA"] = df["PosA"].fillna(0).astype(int).astype(str) df["PosB"] = df["PosB"].fillna(0).astype(int).astype(str) df["PROT_FUSION_TYPE"] = df["PROT_FUSION_TYPE"].replace(".", "nan") @@ -452,7 +474,9 @@ def column_manipulation(df: pd.DataFrame) -> pd.DataFrame: f"EXON_NUMBER_A={row['Left_exon_number']};EXON_NUMBER_B={row['Right_exon_number']};" f"ANNOTATIONS={row['annots']}" ) - df.loc[index, "Sample"] = f"./1:{row['JunctionReadCount']}:{row['SpanningFragCount']}:{row['FFPM']}" + df.loc[index, "Sample"] = ( + f"./1:{row['JunctionReadCount']}:{row['SpanningFragCount']}:{row['FFPM']}" + ) return df @@ -497,7 +521,9 @@ def build_gtf_dataframe(file: str) -> pd.DataFrame: """ df = pd.read_csv(file, sep="\t") df[["fusion_dump", "Transcript_id"]] = df["transcript_id"].str.split("^", expand=True) - df[["orig_chromosome", "orig_start", "orig_end", "orig_dir"]] = df["orig_coord_info"].str.split(",", expand=True) + df[["orig_chromosome", "orig_start", "orig_end", "orig_dir"]] = df["orig_coord_info"].str.split( + ",", expand=True + ) return df[["Transcript_id", "transcript_version", "exon_number", "orig_start", "orig_end"]] @@ -511,7 +537,9 @@ def main(argv=None): or not args.fusionreport_csv or not args.hgnc ): - logger.error(f"The given input file {args.fusioninspector} or {args.fusionreport} was not found!") + logger.error( + f"The given input file {args.fusioninspector} or {args.fusionreport} was not found!" + ) sys.exit(2) vcf_collect( args.fusioninspector, diff --git a/modules/local/picard/collectrnaseqmetrics/main.nf b/modules/local/picard/collectrnaseqmetrics/main.nf index af0b8958..e633e563 100644 --- a/modules/local/picard/collectrnaseqmetrics/main.nf +++ b/modules/local/picard/collectrnaseqmetrics/main.nf @@ -2,10 +2,10 @@ process PICARD_COLLECTRNASEQMETRICS { tag "$meta.id" label 'process_medium' - conda "bioconda::picard=3.0.0 r::r-base" + conda "bioconda::picard=3.1.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/picard:3.0.0--hdfd78af_1' : - 'biocontainers/picard:3.0.0--hdfd78af_1' }" + 'https://depot.galaxyproject.org/singularity/picard:3.1.0--hdfd78af_0' : + 'biocontainers/picard:3.1.0--hdfd78af_0' }" input: tuple val(meta), path(bam), path(bai) @@ -33,15 +33,15 @@ process PICARD_COLLECTRNASEQMETRICS { def rrna = rrna_intervals == [] ? '' : "--RIBOSOMAL_INTERVALS ${rrna_intervals}" def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def avail_mem = 3 + def avail_mem = 3072 if (!task.memory) { log.info '[Picard CollectRnaMetrics] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { - avail_mem = task.memory.giga - } + avail_mem = (task.memory.mega*0.8).intValue() + } """ picard \\ - -Xmx${avail_mem}g \\ + -Xmx${avail_mem}M \\ CollectRnaSeqMetrics \\ --TMP_DIR ./tmp \\ ${strandedness} \\ From 0980e9fab64b6492914ec2c90f3248e7245d7116 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 4 Mar 2024 09:36:46 +0100 Subject: [PATCH 25/64] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 58e8c618..547839a6 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -10,6 +10,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Changed - Update to nf-tools 2.11.1 [#457] (https://github.com/nf-core/rnafusion/pull/457) +- Update picard collectrnaseqmetrics memory requirements to 0.8x what is provided [#474](https://github.com/nf-core/rnafusion/pull/474) ### Fixed From 2cea1a4179fd76185fe471a9398267269fc915ce Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 4 Mar 2024 11:19:01 +0100 Subject: [PATCH 26/64] first step fix --- bin/vcf_collect.py | 7 +++++-- 1 file changed, 5 insertions(+), 2 deletions(-) diff --git a/bin/vcf_collect.py b/bin/vcf_collect.py index 61283d3b..00e5fdb8 100755 --- a/bin/vcf_collect.py +++ b/bin/vcf_collect.py @@ -352,7 +352,8 @@ def read_build_fusionreport(fusionreport_file: str) -> pd.DataFrame: with open(fusionreport_file) as f: from_html = [line.split('rows": [')[1] for line in f if 'name="fusion_list' in line] expression = ast.literal_eval(from_html[0].split('], "tool')[0]) - fusion_report = pd.DataFrame.from_dict({k: [v] for k, v in expression.items()}) + # print(expression) + fusion_report = pd.DataFrame(list(expression)) if not "arriba" in fusion_report.columns: fusion_report["arriba"] = "" if not "fusioncatcher" in fusion_report.columns: @@ -371,8 +372,10 @@ def read_build_fusionreport(fusionreport_file: str) -> pd.DataFrame: fusion_report["FOUND_IN"] = fusion_report[["arriba", "starfusion", "fusioncatcher"]].apply( concatenate_columns, axis=1 ) + print(fusion_report) fusion_report.columns = fusion_report.columns.str.upper() - fusion_report["FOUND_DB"] = fusion_report["FOUND_DB"].apply(lambda x: ",".join(x)) + print(fusion_report["FOUND_DB"]) + fusion_report["FOUND_DB"] = fusion_report["FOUND_DB"].apply(lambda x: ",".join(x) if len(x) > 0 else '') fusion_report[["GeneA", "GeneB"]] = fusion_report["FUSION"].str.split("--", expand=True) return fusion_report[ From 840c0f21c6f5ad570e1e37c58d974a96be1867ea Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 4 Mar 2024 13:10:16 +0100 Subject: [PATCH 27/64] pre-commit for template merge 2.13.1 --- lib/NfcoreTemplate.groovy | 356 -------------- lib/Utils.groovy | 47 -- lib/WorkflowMain.groovy | 76 --- lib/WorkflowRnafusion.groovy | 122 ----- modules.json | 19 + .../utils_nfcore_rnafusion_pipeline/main.nf | 286 ++++++++++++ .../nf-core/utils_nextflow_pipeline/main.nf | 126 +++++ .../nf-core/utils_nextflow_pipeline/meta.yml | 38 ++ .../tests/main.function.nf.test | 54 +++ .../tests/main.function.nf.test.snap | 20 + .../tests/main.workflow.nf.test | 111 +++++ .../tests/nextflow.config | 9 + .../utils_nextflow_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfcore_pipeline/main.nf | 440 ++++++++++++++++++ .../nf-core/utils_nfcore_pipeline/meta.yml | 24 + .../tests/main.function.nf.test | 134 ++++++ .../tests/main.function.nf.test.snap | 166 +++++++ .../tests/main.workflow.nf.test | 29 ++ .../tests/main.workflow.nf.test.snap | 19 + .../tests/nextflow.config | 9 + .../utils_nfcore_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfvalidation_plugin/main.nf | 62 +++ .../utils_nfvalidation_plugin/meta.yml | 44 ++ .../tests/main.nf.test | 200 ++++++++ .../tests/nextflow_schema.json | 96 ++++ .../utils_nfvalidation_plugin/tests/tags.yml | 2 + workflows/rnafusion.nf | 57 ++- 27 files changed, 1926 insertions(+), 624 deletions(-) delete mode 100755 lib/NfcoreTemplate.groovy delete mode 100644 lib/Utils.groovy delete mode 100755 lib/WorkflowMain.groovy delete mode 100755 lib/WorkflowRnafusion.groovy create mode 100644 subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy deleted file mode 100755 index e248e4c3..00000000 --- a/lib/NfcoreTemplate.groovy +++ /dev/null @@ -1,356 +0,0 @@ -// -// This file holds several functions used within the nf-core pipeline template. -// - -import org.yaml.snakeyaml.Yaml -import groovy.json.JsonOutput -import nextflow.extension.FilesEx - -class NfcoreTemplate { - - // - // Check AWS Batch related parameters have been specified correctly - // - public static void awsBatch(workflow, params) { - if (workflow.profile.contains('awsbatch')) { - // Check params.awsqueue and params.awsregion have been set if running on AWSBatch - assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - } - } - - // - // Warn if a -profile or Nextflow config has not been provided to run the pipeline - // - public static void checkConfigProvided(workflow, log) { - if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " - } - } - - // - // Generate version string - // - public static String version(workflow) { - String version_string = "" - - if (workflow.manifest.version) { - def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' - version_string += "${prefix_v}${workflow.manifest.version}" - } - - if (workflow.commitId) { - def git_shortsha = workflow.commitId.substring(0, 7) - version_string += "-g${git_shortsha}" - } - - return version_string - } - - // - // Construct and send completion email - // - public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['Date Started'] = workflow.start - misc_fields['Date Completed'] = workflow.complete - misc_fields['Pipeline script file path'] = workflow.scriptFile - misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build - misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - def email_fields = [:] - email_fields['version'] = NfcoreTemplate.version(workflow) - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary << misc_fields - - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { - if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" - } - mqc_report = mqc_report[0] - } - } - } catch (all) { - if (multiqc_report) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" - } - } - - // Check if we are only sending emails on failure - def email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - Map colors = logColours(params.monochrome_logs) - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") - sendmail_tf.withWriter { w -> w << sendmail_html } - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" - } - } - - // Write summary e-mail HTML to a file - def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); - output_hf.delete() - - // Write summary e-mail TXT to a file - def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); - output_tf.delete() - } - - // - // Construct and send a notification to a web server as JSON - // e.g. Microsoft Teams and Slack - // - public static void IM_notification(workflow, params, summary_params, projectDir, log) { - def hook_url = params.hook_url - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp - - def msg_fields = [:] - msg_fields['version'] = NfcoreTemplate.version(workflow) - msg_fields['runName'] = workflow.runName - msg_fields['success'] = workflow.success - msg_fields['dateComplete'] = workflow.complete - msg_fields['duration'] = workflow.duration - msg_fields['exitStatus'] = workflow.exitStatus - msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - msg_fields['errorReport'] = (workflow.errorReport ?: 'None') - msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") - msg_fields['projectDir'] = workflow.projectDir - msg_fields['summary'] = summary << misc_fields - - // Render the JSON template - def engine = new groovy.text.GStringTemplateEngine() - // Different JSON depending on the service provider - // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format - def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" - def hf = new File("$projectDir/assets/${json_path}") - def json_template = engine.createTemplate(hf).make(msg_fields) - def json_message = json_template.toString() - - // POST - def post = new URL(hook_url).openConnection(); - post.setRequestMethod("POST") - post.setDoOutput(true) - post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); - } - } - - // - // Dump pipeline parameters in a json file - // - public static void dump_parameters(workflow, params) { - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def filename = "params_${timestamp}.json" - def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = JsonOutput.toJson(params) - temp_pf.text = JsonOutput.prettyPrint(jsonStr) - - FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") - temp_pf.delete() - } - - // - // Print pipeline summary on completion - // - public static void summary(workflow, params, log) { - Map colors = logColours(params.monochrome_logs) - if (workflow.success) { - if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" - } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" - } - } - - // - // ANSII Colours used for terminal logging - // - public static Map logColours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - // - // Does what is says on the tin - // - public static String dashedLine(monochrome_logs) { - Map colors = logColours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - // - // nf-core logo - // - public static String logo(workflow, monochrome_logs) { - Map colors = logColours(monochrome_logs) - String workflow_version = NfcoreTemplate.version(workflow) - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/lib/Utils.groovy b/lib/Utils.groovy deleted file mode 100644 index 8d030f4e..00000000 --- a/lib/Utils.groovy +++ /dev/null @@ -1,47 +0,0 @@ -// -// This file holds several Groovy functions that could be useful for any Nextflow pipeline -// - -import org.yaml.snakeyaml.Yaml - -class Utils { - - // - // When running with -profile conda, warn if channels have not been set-up appropriately - // - public static void checkCondaChannels(log) { - Yaml parser = new Yaml() - def channels = [] - try { - def config = parser.load("conda config --show channels".execute().text) - channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return - } - - // Check that all channels are present - // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] - def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean - - // Check that they are in the right order - def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) - } - - if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - } - } -} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy deleted file mode 100755 index 550682a3..00000000 --- a/lib/WorkflowMain.groovy +++ /dev/null @@ -1,76 +0,0 @@ -// -// This file holds several functions specific to the main.nf workflow in the nf-core/rnafusion pipeline -// - -import nextflow.Nextflow - -class WorkflowMain { - - // - // Citation string for pipeline - // - public static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - "* The pipeline\n" + - " https://doi.org/10.5281/zenodo.151721952\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" - } - - - // - // Validate parameters and print summary to screen - // - public static void initialise(workflow, params, log, args) { - - // Print workflow version and exit on --version - if (params.version) { - String workflow_version = NfcoreTemplate.version(workflow) - log.info "${workflow.manifest.name} ${workflow_version}" - System.exit(0) - } - - // Check that a -profile or Nextflow config has been provided to run the pipeline - NfcoreTemplate.checkConfigProvided(workflow, log) - // Check that the profile doesn't contain spaces and doesn't end with a trailing comma - checkProfile(workflow.profile, args, log) - - // Check that conda channels are set-up correctly - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - Utils.checkCondaChannels(log) - } - - // Check AWS batch settings - NfcoreTemplate.awsBatch(workflow, params) - - // Check input has been provided - if (!params.input) { - Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") - } - } - // - // Get attribute from genome config file e.g. fasta - // - public static Object getGenomeAttribute(params, attribute) { - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - return params.genomes[ params.genome ][ attribute ] - } - } - return null - } - - // - // Exit pipeline if --profile contains spaces - // - private static void checkProfile(profile, args, log) { - if (profile.endsWith(',')) { - Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - if (args[0]) { - log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - } -} diff --git a/lib/WorkflowRnafusion.groovy b/lib/WorkflowRnafusion.groovy deleted file mode 100755 index 1e289fdc..00000000 --- a/lib/WorkflowRnafusion.groovy +++ /dev/null @@ -1,122 +0,0 @@ -// -// This file holds several functions specific to the workflow/rnafusion.nf in the nf-core/rnafusion pipeline -// - -import nextflow.Nextflow -import groovy.text.SimpleTemplateEngine - -class WorkflowRnafusion { - - // - // Check and validate parameters - // - public static void initialise(params, log) { - - genomeExistsError(params, log) - - - if (!params.fasta) { - Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - } - } - - // - // Get workflow summary for MultiQC - // - public static String paramsSummaryMultiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

    $group

    \n" - summary_section += "
    \n" - for (param in group_params.keySet()) { - summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" - } - summary_section += "
    \n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } - - // - // Generate methods description for MultiQC - // - - public static String toolCitationText(params) { - - // TODO nf-core: Optionally add in-text citation tools to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def citation_text = [ - "Tools used in the workflow included:", - "FastQC (Andrews 2010),", - "MultiQC (Ewels et al. 2016)", - "." - ].join(' ').trim() - - return citation_text - } - - public static String toolBibliographyText(params) { - - // TODO Optionally add bibliographic entries to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " - ].join(' ').trim() - - return reference_text - } - - public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { - // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file - def meta = [:] - meta.workflow = run_workflow.toMap() - meta["manifest_map"] = run_workflow.manifest.toMap() - - // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " - - // Tool references - meta["tool_citations"] = "" - meta["tool_bibliography"] = "" - - // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - //meta["tool_bibliography"] = toolBibliographyText(params) - - - def methods_text = mqc_methods_yaml.text - - def engine = new SimpleTemplateEngine() - def description_html = engine.createTemplate(methods_text).make(meta) - - return description_html - } - - // - // Exit pipeline if incorrect --genome key provided - // - private static void genomeExistsError(params, log) { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + - " Currently, the available genome keys are:\n" + - " ${params.genomes.keySet().join(", ")}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - Nextflow.error(error_string) - } - } -} diff --git a/modules.json b/modules.json index d5c7ee3d..a926bbcb 100644 --- a/modules.json +++ b/modules.json @@ -111,6 +111,25 @@ "installed_by": ["modules"] } } + }, + "subworkflows": { + "nf-core": { + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": ["subworkflows"] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": ["subworkflows"] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": ["subworkflows"] + } + } } } } diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf new file mode 100644 index 00000000..dec3e3d8 --- /dev/null +++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf @@ -0,0 +1,286 @@ +// +// Subworkflow with functionality specific to the nf-core/rnafusion pipeline +// + +import groovy.json.JsonSlurper + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' +include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' +include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' +include { nfCoreLogo } from '../../nf-core/utils_nfcore_pipeline' +include { imNotification } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' +include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' + +/* +======================================================================================== + SUBWORKFLOW TO INITIALISE PIPELINE +======================================================================================== +*/ + +workflow PIPELINE_INITIALISATION { + + take: + version // boolean: Display version and exit + help // boolean: Display help text + validate_params // boolean: Boolean whether to validate parameters against the schema at runtime + monochrome_logs // boolean: Do not use coloured log outputs + nextflow_cli_args // array: List of positional nextflow CLI args + outdir // string: The output directory where the results will be saved + + main: + + // + // Print version and exit if required and dump pipeline parameters to JSON file + // + UTILS_NEXTFLOW_PIPELINE ( + version, + true, + outdir, + workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 + ) + + // + // Validate parameters and generate parameter summary to stdout + // + pre_help_text = nfCoreLogo(monochrome_logs) + post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) + def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input samplesheet.csv --genome_base } --outdir " + UTILS_NFVALIDATION_PLUGIN ( + help, + workflow_command, + pre_help_text, + post_help_text, + validate_params, + "nextflow_schema.json" + ) + + // + // Check config provided to the pipeline + // + UTILS_NFCORE_PIPELINE ( + nextflow_cli_args + ) + + // + // Custom validation for pipeline parameters + // + validateInputParameters() + +} + +/* +======================================================================================== + SUBWORKFLOW FOR PIPELINE COMPLETION +======================================================================================== +*/ + +workflow PIPELINE_COMPLETION { + + take: + email // string: email address + email_on_fail // string: email address sent on pipeline failure + plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published + monochrome_logs // boolean: Disable ANSI colour codes in log output + hook_url // string: hook URL for notifications + multiqc_report // string: Path to MultiQC report + + main: + + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + + // + // Completion email and summary + // + workflow.onComplete { + if (email || email_on_fail) { + completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + } + + completionSummary(monochrome_logs) + + if (hook_url) { + imNotification(summary_params, hook_url) + } + } +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Function to validate channels from input samplesheet +// +def validateInputSamplesheet(input) { + def (metas, fastqs) = input[1..2] + + // Check that multiple runs of the same sample are of the same strandedness + def strandedness_ok = metas.collect{ it.strandedness }.unique().size == 1 + if (!strandedness_ok) { + error("Please check input samplesheet -> Multiple runs of a sample must have the same strandedness!: ${metas[0].id}") + } + + // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end + def endedness_ok = metas.collect{ it.single_end }.unique().size == 1 + if (!endedness_ok) { + error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") + } + + return [ metas[0], fastqs ] +} + +// +// Check and validate pipeline parameters +// +def validateInputParameters() { + + genomeExistsError() +} + +// +// Get attribute from genome config file e.g. fasta +// +def getGenomeAttribute(attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null +} + +// +// Exit pipeline if incorrect --genome key provided +// +def genomeExistsError() { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + error(error_string) + } +} + +// +// Generate methods description for MultiQC +// +def toolCitationText() { + // TODO nf-core: Optionally add in-text citation tools to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def citation_text = [ + "Tools used in the workflow included:", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text +} + +def toolBibliographyText() { + // TODO nf-core: Optionally add bibliographic entries to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def reference_text = [ + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text +} + +def methodsDescriptionText(mqc_methods_yaml) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = workflow.toMap() + meta["manifest_map"] = workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = "" + meta["tool_bibliography"] = "" + + // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! + // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + // meta["tool_bibliography"] = toolBibliographyText() + def methods_text = mqc_methods_yaml.text + + def engine = new groovy.text.SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html.toString() +} + +// +// Function to generate an error if contigs in genome fasta file > 512 Mbp +// +def checkMaxContigSize(fai_file) { + def max_size = 512000000 + fai_file.eachLine { line -> + def lspl = line.split('\t') + def chrom = lspl[0] + def size = lspl[1] + if (size.toInteger() > max_size) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Contig longer than ${max_size}bp found in reference genome!\n\n" + + " ${chrom}: ${size}\n\n" + + " Provide the '--bam_csi_index' parameter to use a CSI instead of BAI index.\n\n" + + " Please see:\n" + + " https://github.com/nf-core/rnaseq/issues/744\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + error(error_string) + } + } +} + +// +// Create MultiQC tsv custom content from a list of values +// +def multiqcTsvFromList(tsv_data, header) { + def tsv_string = "" + if (tsv_data.size() > 0) { + tsv_string += "${header.join('\t')}\n" + tsv_string += tsv_data.join('\n') + } + return tsv_string +} + + +// +// Function that parses and returns the alignment rate from the STAR log output +// +def getStarPercentMapped(params, align_log) { + def percent_aligned = 0 + def pattern = /Uniquely mapped reads %\s*\|\s*([\d\.]+)%/ + align_log.eachLine { line -> + def matcher = line =~ pattern + if (matcher) { + percent_aligned = matcher[0][1].toFloat() + } + } + + def pass = false + if (percent_aligned >= params.min_mapped_reads.toFloat()) { + pass = true + } + return [ percent_aligned, pass ] +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf new file mode 100644 index 00000000..ac31f28f --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -0,0 +1,126 @@ +// +// Subworkflow with functionality that may be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NEXTFLOW_PIPELINE { + + take: + print_version // boolean: print version + dump_parameters // boolean: dump parameters + outdir // path: base directory used to publish pipeline results + check_conda_channels // boolean: check conda channels + + main: + + // + // Print workflow version and exit on --version + // + if (print_version) { + log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + System.exit(0) + } + + // + // Dump pipeline parameters to a JSON file + // + if (dump_parameters && outdir) { + dumpParametersToJSON(outdir) + } + + // + // When running with Conda, warn if channels have not been set-up appropriately + // + if (check_conda_channels) { + checkCondaChannels() + } + + emit: + dummy_emit = true +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Generate version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Dump pipeline parameters to a JSON file +// +def dumpParametersToJSON(outdir) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() +} + +// +// When running with -profile conda, warn if channels have not been set-up appropriately +// +def checkCondaChannels() { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml new file mode 100644 index 00000000..e5c3a0a8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml @@ -0,0 +1,38 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NEXTFLOW_PIPELINE" +description: Subworkflow with functionality that may be useful for any Nextflow pipeline +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - print_version: + type: boolean + description: | + Print the version of the pipeline and exit + - dump_parameters: + type: boolean + description: | + Dump the parameters of the pipeline to a JSON file + - output_directory: + type: directory + description: Path to output dir to write JSON file to. + pattern: "results/" + - check_conda_channel: + type: boolean + description: | + Check if the conda channel priority is correct. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" + - "@drpatelh" +maintainers: + - "@adamrtalbot" + - "@drpatelh" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..68718e4f --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -0,0 +1,54 @@ + +nextflow_function { + + name "Test Functions" + script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Test Function getWorkflowVersion") { + + function "getWorkflowVersion" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dumpParametersToJSON") { + + function "dumpParametersToJSON" + + when { + function { + """ + // define inputs of the function here. Example: + input[0] = "$outputDir" + """.stripIndent() + } + } + + then { + assertAll( + { assert function.success } + ) + } + } + + test("Test Function checkCondaChannels") { + + function "checkCondaChannels" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..e3f0baf4 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,20 @@ +{ + "Test Function getWorkflowVersion": { + "content": [ + "v9.9.9" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:05.308243" + }, + "Test Function checkCondaChannels": { + "content": null, + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:12.425833" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..ca964ce8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,111 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NEXTFLOW_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + workflow "UTILS_NEXTFLOW_PIPELINE" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Should run no inputs") { + + when { + workflow { + """ + print_version = false + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should print version") { + + when { + workflow { + """ + print_version = true + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.stdout.contains("nextflow_workflow v9.9.9") } + ) + } + } + + test("Should dump params") { + + when { + workflow { + """ + print_version = false + dump_parameters = true + outdir = 'results' + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should not create params JSON if no output directory") { + + when { + workflow { + """ + print_version = false + dump_parameters = true + outdir = null + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config new file mode 100644 index 00000000..d0a926bf --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml new file mode 100644 index 00000000..f8476112 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nextflow_pipeline: + - subworkflows/nf-core/utils_nextflow_pipeline/** diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf new file mode 100644 index 00000000..a8b55d6f --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -0,0 +1,440 @@ +// +// Subworkflow with utility functions specific to the nf-core pipeline template +// + +import org.yaml.snakeyaml.Yaml +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFCORE_PIPELINE { + + take: + nextflow_cli_args + + main: + valid_config = checkConfigProvided() + checkProfileProvided(nextflow_cli_args) + + emit: + valid_config +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Warn if a -profile or Nextflow config has not been provided to run the pipeline +// +def checkConfigProvided() { + valid_config = true + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + valid_config = false + } + return valid_config +} + +// +// Exit pipeline if --profile contains spaces +// +def checkProfileProvided(nextflow_cli_args) { + if (workflow.profile.endsWith(',')) { + error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } + if (nextflow_cli_args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } +} + +// +// Citation string for pipeline +// +def workflowCitation() { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + " ${workflow.manifest.doi}\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" +} + +// +// Generate workflow version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Get software versions for pipeline +// +def processVersionsFromYAML(yaml_file) { + Yaml yaml = new Yaml() + versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + return yaml.dumpAsMap(versions).trim() +} + +// +// Get workflow version for pipeline +// +def workflowVersionToYAML() { + return """ + Workflow: + $workflow.manifest.name: ${getWorkflowVersion()} + Nextflow: $workflow.nextflow.version + """.stripIndent().trim() +} + +// +// Get channel of software versions used in pipeline in YAML format +// +def softwareVersionsToYAML(ch_versions) { + return ch_versions + .unique() + .map { processVersionsFromYAML(it) } + .unique() + .mix(Channel.of(workflowVersionToYAML())) +} + +// +// Get workflow summary for MultiQC +// +def paramsSummaryMultiqc(summary_params) { + def summary_section = '' + for (group in summary_params.keySet()) { + def group_params = summary_params.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + + return yaml_file_text +} + +// +// nf-core logo +// +def nfCoreLogo(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) +} + +// +// Return dashed line +// +def dashedLine(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" +} + +// +// ANSII colours used for terminal logging +// +def logColours(monochrome_logs=true) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes +} + +// +// Attach the multiqc report to email +// +def attachMultiqcReport(multiqc_report) { + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + return mqc_report +} + +// +// Construct and send completion email +// +def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = getWorkflowVersion() + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = attachMultiqcReport(multiqc_report) + + // Check if we are only sending emails on failure + def email_address = email + if (!email && email_on_fail && !workflow.success) { + email_address = email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("${workflow.projectDir}/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("${workflow.projectDir}/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(monochrome_logs) + if (email_address) { + try { + if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() +} + +// +// Print pipeline summary on completion +// +def completionSummary(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } +} + +// +// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack +// +def imNotification(summary_params, hook_url) { + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = getWorkflowVersion() + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("${workflow.projectDir}/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml new file mode 100644 index 00000000..d08d2434 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml @@ -0,0 +1,24 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFCORE_PIPELINE" +description: Subworkflow with utility functions specific to the nf-core pipeline template +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - nextflow_cli_args: + type: list + description: | + Nextflow CLI positional arguments +output: + - success: + type: boolean + description: | + Dummy output to indicate success +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..1dc317f8 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test @@ -0,0 +1,134 @@ + +nextflow_function { + + name "Test Functions" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Test Function checkConfigProvided") { + + function "checkConfigProvided" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function checkProfileProvided") { + + function "checkProfileProvided" + + when { + function { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function workflowCitation") { + + function "workflowCitation" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function nfCoreLogo") { + + function "nfCoreLogo" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dashedLine") { + + function "dashedLine" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function without logColours") { + + function "logColours" + + when { + function { + """ + input[0] = true + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function with logColours") { + function "logColours" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..1037232c --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,166 @@ +{ + "Test Function checkProfileProvided": { + "content": null, + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:03.360873" + }, + "Test Function checkConfigProvided": { + "content": [ + true + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:59.729647" + }, + "Test Function nfCoreLogo": { + "content": [ + "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:10.562934" + }, + "Test Function workflowCitation": { + "content": [ + "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:07.019761" + }, + "Test Function without logColours": { + "content": [ + { + "reset": "", + "bold": "", + "dim": "", + "underlined": "", + "blink": "", + "reverse": "", + "hidden": "", + "black": "", + "red": "", + "green": "", + "yellow": "", + "blue": "", + "purple": "", + "cyan": "", + "white": "", + "bblack": "", + "bred": "", + "bgreen": "", + "byellow": "", + "bblue": "", + "bpurple": "", + "bcyan": "", + "bwhite": "", + "ublack": "", + "ured": "", + "ugreen": "", + "uyellow": "", + "ublue": "", + "upurple": "", + "ucyan": "", + "uwhite": "", + "iblack": "", + "ired": "", + "igreen": "", + "iyellow": "", + "iblue": "", + "ipurple": "", + "icyan": "", + "iwhite": "", + "biblack": "", + "bired": "", + "bigreen": "", + "biyellow": "", + "biblue": "", + "bipurple": "", + "bicyan": "", + "biwhite": "" + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:17.969323" + }, + "Test Function dashedLine": { + "content": [ + "-\u001b[2m----------------------------------------------------\u001b[0m-" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:14.366181" + }, + "Test Function with logColours": { + "content": [ + { + "reset": "\u001b[0m", + "bold": "\u001b[1m", + "dim": "\u001b[2m", + "underlined": "\u001b[4m", + "blink": "\u001b[5m", + "reverse": "\u001b[7m", + "hidden": "\u001b[8m", + "black": "\u001b[0;30m", + "red": "\u001b[0;31m", + "green": "\u001b[0;32m", + "yellow": "\u001b[0;33m", + "blue": "\u001b[0;34m", + "purple": "\u001b[0;35m", + "cyan": "\u001b[0;36m", + "white": "\u001b[0;37m", + "bblack": "\u001b[1;30m", + "bred": "\u001b[1;31m", + "bgreen": "\u001b[1;32m", + "byellow": "\u001b[1;33m", + "bblue": "\u001b[1;34m", + "bpurple": "\u001b[1;35m", + "bcyan": "\u001b[1;36m", + "bwhite": "\u001b[1;37m", + "ublack": "\u001b[4;30m", + "ured": "\u001b[4;31m", + "ugreen": "\u001b[4;32m", + "uyellow": "\u001b[4;33m", + "ublue": "\u001b[4;34m", + "upurple": "\u001b[4;35m", + "ucyan": "\u001b[4;36m", + "uwhite": "\u001b[4;37m", + "iblack": "\u001b[0;90m", + "ired": "\u001b[0;91m", + "igreen": "\u001b[0;92m", + "iyellow": "\u001b[0;93m", + "iblue": "\u001b[0;94m", + "ipurple": "\u001b[0;95m", + "icyan": "\u001b[0;96m", + "iwhite": "\u001b[0;97m", + "biblack": "\u001b[1;90m", + "bired": "\u001b[1;91m", + "bigreen": "\u001b[1;92m", + "biyellow": "\u001b[1;93m", + "biblue": "\u001b[1;94m", + "bipurple": "\u001b[1;95m", + "bicyan": "\u001b[1;96m", + "biwhite": "\u001b[1;97m" + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:21.714424" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..8940d32d --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,29 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFCORE_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + workflow "UTILS_NFCORE_PIPELINE" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Should run without failures") { + + when { + workflow { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot(workflow.out).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap new file mode 100644 index 00000000..859d1030 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -0,0 +1,19 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + true + ], + "valid_config": [ + true + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:25.726491" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config new file mode 100644 index 00000000..d0a926bf --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml new file mode 100644 index 00000000..ac8523c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfcore_pipeline: + - subworkflows/nf-core/utils_nfcore_pipeline/** diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf new file mode 100644 index 00000000..2585b65d --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf @@ -0,0 +1,62 @@ +// +// Subworkflow that uses the nf-validation plugin to render help text and parameter summary +// + +/* +======================================================================================== + IMPORT NF-VALIDATION PLUGIN +======================================================================================== +*/ + +include { paramsHelp } from 'plugin/nf-validation' +include { paramsSummaryLog } from 'plugin/nf-validation' +include { validateParameters } from 'plugin/nf-validation' + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFVALIDATION_PLUGIN { + + take: + print_help // boolean: print help + workflow_command // string: default commmand used to run pipeline + pre_help_text // string: string to be printed before help text and summary log + post_help_text // string: string to be printed after help text and summary log + validate_params // boolean: validate parameters + schema_filename // path: JSON schema file, null to use default value + + main: + + log.debug "Using schema file: ${schema_filename}" + + // Default values for strings + pre_help_text = pre_help_text ?: '' + post_help_text = post_help_text ?: '' + workflow_command = workflow_command ?: '' + + // + // Print help message if needed + // + if (print_help) { + log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text + System.exit(0) + } + + // + // Print parameter summary to stdout + // + log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text + + // + // Validate parameters relative to the parameter JSON schema + // + if (validate_params){ + validateParameters(parameters_schema: schema_filename) + } + + emit: + dummy_emit = true +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml new file mode 100644 index 00000000..3d4a6b04 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml @@ -0,0 +1,44 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFVALIDATION_PLUGIN" +description: Use nf-validation to initiate and validate a pipeline +keywords: + - utility + - pipeline + - initialise + - validation +components: [] +input: + - print_help: + type: boolean + description: | + Print help message and exit + - workflow_command: + type: string + description: | + The command to run the workflow e.g. "nextflow run main.nf" + - pre_help_text: + type: string + description: | + Text to print before the help message + - post_help_text: + type: string + description: | + Text to print after the help message + - validate_params: + type: boolean + description: | + Validate the parameters and error if invalid. + - schema_filename: + type: string + description: | + The filename of the schema to validate against. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test new file mode 100644 index 00000000..5784a33f --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -0,0 +1,200 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFVALIDATION_PLUGIN" + script "../main.nf" + workflow "UTILS_NFVALIDATION_PLUGIN" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "plugin/nf-validation" + tag "'plugin/nf-validation'" + tag "utils_nfvalidation_plugin" + tag "subworkflows/utils_nfvalidation_plugin" + + test("Should run nothing") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should run help") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with command") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with extra text") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = "pre-help-text" + post_help_text = "post-help-text" + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('pre-help-text') } }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } }, + { assert workflow.stdout.any { it.contains('post-help-text') } } + ) + } + } + + test("Should validate params") { + + when { + + params { + monochrome_logs = true + test_data = '' + outdir = 1 + } + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = true + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json new file mode 100644 index 00000000..7626c1c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json @@ -0,0 +1,96 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", + "title": ". pipeline parameters", + "description": "", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["outdir"], + "properties": { + "validate_params": { + "type": "boolean", + "description": "Validate parameters?", + "default": true, + "hidden": true + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "test_data_base": { + "type": "string", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules", + "description": "Base for test data directory", + "hidden": true + }, + "test_data": { + "type": "string", + "description": "Fake test data param", + "hidden": true + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "logo": { + "type": "boolean", + "default": true, + "description": "Display nf-core logo in console output.", + "fa_icon": "fas fa-image", + "hidden": true + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Pull Singularity container from Docker?", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Use monochrome_logs", + "hidden": true + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml new file mode 100644 index 00000000..60b1cfff --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfvalidation_plugin: + - subworkflows/nf-core/utils_nfvalidation_plugin/** diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf index 1cdc6bfb..d3a1d114 100644 --- a/workflows/rnafusion.nf +++ b/workflows/rnafusion.nf @@ -17,6 +17,8 @@ WorkflowRnafusion.initialise(params, log) // Check mandatory parameters + +//TODO: move this to utils_nf_core_rnafusion_pipeline if (file(params.input).exists() || params.build_references) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet does not exist or was not specified!' } if (params.fusioninspector_only && !params.fusioninspector_fusions) { exit 1, 'Parameter --fusioninspector_fusions PATH_TO_FUSION_LIST expected with parameter --fusioninspector_only'} if (params.tools_cutoff < 1) { exit 1, 'Parameter: --tools_cutoff should be >= 1'} @@ -88,6 +90,8 @@ include { FUSIONCATCHER_WORKFLOW } from '../subworkflows/local/fusionca include { FUSIONINSPECTOR_WORKFLOW } from '../subworkflows/local/fusioninspector_workflow' include { FUSIONREPORT_WORKFLOW } from '../subworkflows/local/fusionreport_workflow' +include { validateInputSamplesheet } from '../../subworkflows/local/utils_nfcore_rnaseq_pipeline' + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT NF-CORE MODULES/SUBWORKFLOWS @@ -118,31 +122,35 @@ workflow RNAFUSION { ch_versions = Channel.empty() - - // - // SUBWORKFLOW: Read in samplesheet, validate and stage input files + // Create channel from input file provided through params.input // - INPUT_CHECK ( - file(params.input) - ) - .reads - .map { - meta, fastq -> - def meta_clone = meta.clone() - meta_clone.id = meta_clone.id.split('_')[0..-2].join('_') - [ meta_clone, fastq ] } - .groupTuple(by: [0]) - .branch { - meta, fastq -> - single : fastq.size() == 1 - return [ meta, fastq.flatten() ] - multiple: fastq.size() > 1 - return [ meta, fastq.flatten() ] - } - .set { ch_fastq } - ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) + Channel + .fromSamplesheet("input") + .map { + meta, fastq_1, fastq_2 -> + if (!fastq_2) { + return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] + } else { + return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] + } + } + .groupTuple() + .map { + validateInputSamplesheet(it) + } + .branch { + meta, fastqs -> + single : fastqs.size() == 1 + return [ meta, fastqs.flatten() ] + multiple: fastqs.size() > 1 + return [ meta, fastqs.flatten() ] + } + .set { ch_fastq } + // + // MODULE: Concatenate FastQ files from same sample if required + // CAT_FASTQ ( ch_fastq.multiple ) @@ -167,7 +175,10 @@ workflow RNAFUSION { ch_reads_all = TRIM_WORKFLOW.out.ch_reads_all ch_versions = ch_versions.mix(TRIM_WORKFLOW.out.versions) - // Run STAR alignment and Arriba + // + // SUBWORKFLOW: Run STAR alignment and Arriba + // + ARRIBA_WORKFLOW ( ch_reads_all, ch_gtf, From 3e8a300b11bdf2085fd4f16a1b9e91daf10394a9 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 4 Mar 2024 13:28:37 +0100 Subject: [PATCH 28/64] first changes --- main.nf | 24 ++++++++++++++++-------- 1 file changed, 16 insertions(+), 8 deletions(-) diff --git a/main.nf b/main.nf index 3a8dfc57..396ae487 100644 --- a/main.nf +++ b/main.nf @@ -13,17 +13,25 @@ nextflow.enable.dsl = 2 /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - GENOME PARAMETER VALUES + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') -params.fai = WorkflowMain.getGenomeAttribute(params, 'fai') -params.gtf = WorkflowMain.getGenomeAttribute(params, 'gtf') -params.chrgtf = WorkflowMain.getGenomeAttribute(params, 'chrgtf') -params.transcript = WorkflowMain.getGenomeAttribute(params, 'transcript') -params.refflat = WorkflowMain.getGenomeAttribute(params, 'refflat') -params.rrna_intervals = WorkflowMain.getGenomeAttribute(params, 'rrna_intervals') +include { BUILD_REFERENCES } from './workflows/build_references' +include { RNAFUSION } from './workflows/rnafusion' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_rnafusion_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_rnafusion_pipeline' +include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_rnafusion_pipeline' + + + +params.fasta = getGenomeAttribute('fasta') +params.fai = getGenomeAttribute('fai') +params.gtf = getGenomeAttribute('gtf') +params.chrgtf = getGenomeAttribute('chrgtf') +params.transcript = getGenomeAttribute('transcript') +params.refflat = getGenomeAttribute('refflat') +params.rrna_intervals = getGenomeAttribute('rrna_intervals') /* ======================================================================================== From ef0ac08e843fc6f4d1fe6188263409c2b3a00b4d Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 4 Mar 2024 15:01:32 +0100 Subject: [PATCH 29/64] remove genome arg --- nextflow_schema.json | 5 ----- 1 file changed, 5 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 2a311296..5beb02f0 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -284,11 +284,6 @@ "fa_icon": "fas fa-dna", "description": "Reference genome related files and options required for the workflow.", "properties": { - "genome": { - "type": "string", - "description": "Name of iGenomes reference.", - "fa_icon": "fas fa-book" - }, "fasta": { "type": "string", "format": "file-path", From 7f37d02631a0de542246221e6f184c422b38b153 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 4 Mar 2024 15:04:47 +0100 Subject: [PATCH 30/64] move fasta out from genome --- conf/genomes.config | 23 ----------------------- nextflow.config | 15 +++++++++++---- 2 files changed, 11 insertions(+), 27 deletions(-) delete mode 100644 conf/genomes.config diff --git a/conf/genomes.config b/conf/genomes.config deleted file mode 100644 index c1385ebe..00000000 --- a/conf/genomes.config +++ /dev/null @@ -1,23 +0,0 @@ -/* - * ------------------------------------------------- - * Nextflow config file for reference genome - * ------------------------------------------------- - * These references have to be build manually due - * to ERCC spike-ins. - * Can be used by any config that customizes the base - * path using $params.genomes_base / --genomes_base - */ - -params { - genomes { - 'GRCh38' { - fasta = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa" - fai = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa.fai" - gtf = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.gtf" - chrgtf = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf" - transcript = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.cdna.all.fa.gz" - refflat = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf.refflat" - rrna_intervals= "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.interval_list" - } - } -} diff --git a/nextflow.config b/nextflow.config index d2352a8c..8eca3ca0 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,6 +16,8 @@ params { cosmic_passwd = null qiagen = false + + // MultiQC options multiqc_config = null multiqc_title = null @@ -24,13 +26,21 @@ params { multiqc_methods_description = null // Genome - genome = 'GRCh38' genomes_base = "${params.outdir}/references" ensembl_version = 102 read_length = 100 genomes = [:] starfusion_build = true + // Genomes options + fasta = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa" + fai = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa.fai" + gtf = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.gtf" + chrgtf = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf" + transcript = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.cdna.all.fa.gz" + refflat = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf.refflat" + rrna_intervals= "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.interval_list" + // Filtering tools_cutoff = 1 @@ -114,7 +124,6 @@ params { // Schema validation default options validationFailUnrecognisedParams = false validationLenientMode = false - validationSchemaIgnoreParams = 'genomes' validationShowHiddenParams = false validate_params = true } @@ -122,8 +131,6 @@ params { // Load base.config by default for all pipelines includeConfig 'conf/base.config' -includeConfig 'conf/genomes.config' - // Load nf-core custom profiles from different Institutions try { includeConfig "${params.custom_config_base}/nfcore_custom.config" From 49d4ecaf1448af8352b6c31d6bd7089e33337e5b Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 4 Mar 2024 15:16:12 +0100 Subject: [PATCH 31/64] merge fixes --- conf/base.config | 3 --- conf/modules.config | 8 -------- modules.json | 24 ------------------------ nextflow.config | 1 - 4 files changed, 36 deletions(-) diff --git a/conf/base.config b/conf/base.config index 165a5e88..5b2e6f36 100644 --- a/conf/base.config +++ b/conf/base.config @@ -52,7 +52,4 @@ process { errorStrategy = 'retry' maxRetries = 2 } - withName:CUSTOM_DUMPSOFTWAREVERSIONS { - cache = false - } } diff --git a/conf/modules.config b/conf/modules.config index fb0be54e..5bd32d63 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -49,14 +49,6 @@ process { ] } - withName: 'CUSTOM_DUMPSOFTWAREVERSIONS' { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - pattern: '*_versions.yml' - ] - } - withName: 'ENSEMBL_DOWNLOAD' { publishDir = [ path: { "${params.genomes_base}/ensembl" }, diff --git a/modules.json b/modules.json index 4fef058f..23a6ce29 100644 --- a/modules.json +++ b/modules.json @@ -25,11 +25,6 @@ "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] - }, "fastp": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", @@ -112,25 +107,6 @@ } } }, - "subworkflows": { - "nf-core": { - "utils_nextflow_pipeline": { - "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] - }, - "utils_nfcore_pipeline": { - "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] - }, - "utils_nfvalidation_plugin": { - "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] - } - } - }, "subworkflows": { "nf-core": { "utils_nextflow_pipeline": { diff --git a/nextflow.config b/nextflow.config index 8eca3ca0..0341ae61 100644 --- a/nextflow.config +++ b/nextflow.config @@ -29,7 +29,6 @@ params { genomes_base = "${params.outdir}/references" ensembl_version = 102 read_length = 100 - genomes = [:] starfusion_build = true // Genomes options From b64f5ae32fa2886e883ef3aa06c0809f4e685d31 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 4 Mar 2024 15:17:25 +0100 Subject: [PATCH 32/64] merge fixes --- nextflow.config | 1 + 1 file changed, 1 insertion(+) diff --git a/nextflow.config b/nextflow.config index 0341ae61..632565ee 100644 --- a/nextflow.config +++ b/nextflow.config @@ -123,6 +123,7 @@ params { // Schema validation default options validationFailUnrecognisedParams = false validationLenientMode = false + validationSchemaIgnoreParams = '' validationShowHiddenParams = false validate_params = true } From 296bb48a05cf27a9f7741caebdb8d3890d381e91 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 4 Mar 2024 15:48:23 +0100 Subject: [PATCH 33/64] genomes --- nextflow.config | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 632565ee..45d63730 100644 --- a/nextflow.config +++ b/nextflow.config @@ -30,6 +30,7 @@ params { ensembl_version = 102 read_length = 100 starfusion_build = true + genomes = [:] // Genomes options fasta = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa" @@ -123,7 +124,7 @@ params { // Schema validation default options validationFailUnrecognisedParams = false validationLenientMode = false - validationSchemaIgnoreParams = '' + validationSchemaIgnoreParams = 'genomes' validationShowHiddenParams = false validate_params = true } From f551306d2af946b1f0c4c72f6e8e0b62e69a4852 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 4 Mar 2024 15:54:32 +0100 Subject: [PATCH 34/64] add genome --- nextflow.config | 1 + nextflow_schema.json | 5 +++++ 2 files changed, 6 insertions(+) diff --git a/nextflow.config b/nextflow.config index 45d63730..3c22cd82 100644 --- a/nextflow.config +++ b/nextflow.config @@ -26,6 +26,7 @@ params { multiqc_methods_description = null // Genome + genome = 'GRCh38' genomes_base = "${params.outdir}/references" ensembl_version = 102 read_length = 100 diff --git a/nextflow_schema.json b/nextflow_schema.json index 5beb02f0..2a311296 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -284,6 +284,11 @@ "fa_icon": "fas fa-dna", "description": "Reference genome related files and options required for the workflow.", "properties": { + "genome": { + "type": "string", + "description": "Name of iGenomes reference.", + "fa_icon": "fas fa-book" + }, "fasta": { "type": "string", "format": "file-path", From 38fe79f92279fd3fa39ed4ff459bcda8d329f263 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 4 Mar 2024 17:52:04 +0100 Subject: [PATCH 35/64] fix merging --- main.nf | 9 +--- .../utils_nfcore_rnafusion_pipeline/main.nf | 29 +---------- workflows/build_references.nf | 12 ++--- workflows/rnafusion.nf | 48 +++++++------------ 4 files changed, 28 insertions(+), 70 deletions(-) diff --git a/main.nf b/main.nf index 76253a91..6fe53192 100644 --- a/main.nf +++ b/main.nf @@ -35,9 +35,6 @@ include { RNAFUSION } from './workflows/rnafusion' // workflow NFCORE_RNAFUSION { - take: - samplesheet // channel: samplesheet read in from --input - main: // @@ -46,7 +43,7 @@ workflow NFCORE_RNAFUSION { if (params.build_references) { BUILD_REFERENCES () } else { - RNAFUSION(samplesheet) + RNAFUSION() } emit: @@ -79,9 +76,7 @@ workflow { // // WORKFLOW: Run main workflow // - NFCORE_RNAFUSION ( - PIPELINE_INITIALISATION.out.samplesheet - ) + NFCORE_RNAFUSION () // // SUBWORKFLOW: Run completion tasks diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf index a60d3f6b..98b9d7e7 100644 --- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf @@ -2,6 +2,8 @@ // Subworkflow with functionality specific to the nf-core/rnafusion pipeline // +import groovy.json.JsonSlurper + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS @@ -79,33 +81,6 @@ workflow PIPELINE_INITIALISATION { // validateInputParameters() - // - // Create channel from input file provided through params.input - // - Channel - .fromSamplesheet("input") - .map { - meta, fastq_1, fastq_2 -> - if (!fastq_2) { - return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] - } else { - return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] - } - } - .groupTuple() - .map { - validateInputSamplesheet(it) - } - .map { - meta, fastqs -> - return [ meta, fastqs.flatten() ] - } - .set { ch_samplesheet } - - emit: - samplesheet = ch_samplesheet - versions = ch_versions - } /* diff --git a/workflows/build_references.nf b/workflows/build_references.nf index 0ebf3c08..cfbafafe 100644 --- a/workflows/build_references.nf +++ b/workflows/build_references.nf @@ -86,12 +86,12 @@ workflow BUILD_REFERENCES { ======================================================================================== */ -workflow.onComplete { - if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) - } - NfcoreTemplate.summary(workflow, params, log) -} +// workflow.onComplete { +// if (params.email || params.email_on_fail) { +// NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) +// } +// NfcoreTemplate.summary(workflow, params, log) +// } /* ======================================================================================== diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf index c97761e2..98345287 100644 --- a/workflows/rnafusion.nf +++ b/workflows/rnafusion.nf @@ -6,7 +6,6 @@ include { FASTQC } from '../modules/nf-core/fastqc/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { paramsSummaryMap } from 'plugin/nf-validation' include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_rnafusion_pipeline' @@ -24,22 +23,22 @@ include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' // if (params.tools_cutoff < 1) { exit 1, 'Parameter: --tools_cutoff should be >= 1'} -// ch_chrgtf = params.starfusion_build ? Channel.fromPath(params.chrgtf).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_annot.gtf").map { it -> [[id:it.Name], it] }.collect() -// ch_starindex_ref = params.starfusion_build ? Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_genome.fa.star.idx").map { it -> [[id:it.Name], it] }.collect() -// ch_starindex_ensembl_ref = Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect() -// ch_refflat = params.starfusion_build ? Channel.fromPath(params.refflat).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.gtf.refflat").map { it -> [[id:it.Name], it] }.collect() -// ch_rrna_interval = params.starfusion_build ? Channel.fromPath(params.rrna_intervals).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.interval_list").map { it -> [[id:it.Name], it] }.collect() -// ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { it -> [[id:it.Name], it] }.collect() -// ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { it -> [[id:it.Name], it] }.collect() -// ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { it -> [[id:it.Name], it] }.collect() -// ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { it -> [[id:it.Name], it] }.collect() -// ch_arriba_ref_cytobands = Channel.fromPath(params.arriba_ref_cytobands).map { it -> [[id:it.Name], it] }.collect() -// ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { it -> [[id:it.Name], it] }.collect() -// ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { it -> [[id:it.Name], it] }.collect() -// ch_fasta = Channel.fromPath(params.fasta).map { it -> [[id:it.Name], it] }.collect() -// ch_gtf = Channel.fromPath(params.gtf).map { it -> [[id:it.Name], it] }.collect() -// ch_transcript = Channel.fromPath(params.transcript).map { it -> [[id:it.Name], it] }.collect() -// ch_fai = Channel.fromPath(params.fai).map { it -> [[id:it.Name], it] }.collect() +ch_chrgtf = params.starfusion_build ? Channel.fromPath(params.chrgtf).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_annot.gtf").map { it -> [[id:it.Name], it] }.collect() +ch_starindex_ref = params.starfusion_build ? Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_genome.fa.star.idx").map { it -> [[id:it.Name], it] }.collect() +ch_starindex_ensembl_ref = Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect() +ch_refflat = params.starfusion_build ? Channel.fromPath(params.refflat).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.gtf.refflat").map { it -> [[id:it.Name], it] }.collect() +ch_rrna_interval = params.starfusion_build ? Channel.fromPath(params.rrna_intervals).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.ensembl_ref}/ref_annot.interval_list").map { it -> [[id:it.Name], it] }.collect() +ch_fusionreport_ref = Channel.fromPath(params.fusionreport_ref).map { it -> [[id:it.Name], it] }.collect() +ch_arriba_ref_blacklist = Channel.fromPath(params.arriba_ref_blacklist).map { it -> [[id:it.Name], it] }.collect() +ch_arriba_ref_known_fusions = Channel.fromPath(params.arriba_ref_known_fusions).map { it -> [[id:it.Name], it] }.collect() +ch_arriba_ref_protein_domains = Channel.fromPath(params.arriba_ref_protein_domains).map { it -> [[id:it.Name], it] }.collect() +ch_arriba_ref_cytobands = Channel.fromPath(params.arriba_ref_cytobands).map { it -> [[id:it.Name], it] }.collect() +ch_hgnc_ref = Channel.fromPath(params.hgnc_ref).map { it -> [[id:it.Name], it] }.collect() +ch_hgnc_date = Channel.fromPath(params.hgnc_date).map { it -> [[id:it.Name], it] }.collect() +ch_fasta = Channel.fromPath(params.fasta).map { it -> [[id:it.Name], it] }.collect() +ch_gtf = Channel.fromPath(params.gtf).map { it -> [[id:it.Name], it] }.collect() +ch_transcript = Channel.fromPath(params.transcript).map { it -> [[id:it.Name], it] }.collect() +ch_fai = Channel.fromPath(params.fai).map { it -> [[id:it.Name], it] }.collect() // def checkPathParamList = [ // params.fasta, @@ -70,7 +69,6 @@ include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // -include { INPUT_CHECK } from '../subworkflows/local/input_check' include { TRIM_WORKFLOW } from '../subworkflows/local/trim_workflow' include { ARRIBA_WORKFLOW } from '../subworkflows/local/arriba_workflow' include { QC_WORKFLOW } from '../subworkflows/local/qc_workflow' @@ -79,7 +77,7 @@ include { STRINGTIE_WORKFLOW } from '../subworkflows/local/stringti include { FUSIONCATCHER_WORKFLOW } from '../subworkflows/local/fusioncatcher_workflow' include { FUSIONINSPECTOR_WORKFLOW } from '../subworkflows/local/fusioninspector_workflow' include { FUSIONREPORT_WORKFLOW } from '../subworkflows/local/fusionreport_workflow' -include { validateInputSamplesheet } from '../../subworkflows/local/utils_nfcore_rnaseq_pipeline' +include { validateInputSamplesheet } from '../subworkflows/local/utils_nfcore_rnafusion_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -91,9 +89,6 @@ include { validateInputSamplesheet } from '../../subworkflows/local/utils_n // MODULE: Installed directly from nf-core/modules // include { CAT_FASTQ } from '../modules/nf-core/cat/fastq/main' -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -103,9 +98,6 @@ include { MULTIQC } from '../modules/nf-core/multiqc/main' workflow RNAFUSION { - take: - ch_samplesheet // channel: samplesheet read in from --input - main: ch_versions = Channel.empty() @@ -152,15 +144,11 @@ workflow RNAFUSION { // MODULE: Run FastQC // FASTQC ( - ch_samplesheet + ch_cat_fastq ) ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - ch_cat_fastq - ) - ch_versions = ch_versions.mix(FASTQC.out.versions) - TRIM_WORKFLOW ( ch_cat_fastq ) From 3a9e1233be5fe2ab163f80e49b73dac6a72bb4c0 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 4 Mar 2024 18:05:42 +0100 Subject: [PATCH 36/64] fix merging --- main.nf | 4 ---- subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf | 3 +-- 2 files changed, 1 insertion(+), 6 deletions(-) diff --git a/main.nf b/main.nf index 6fe53192..8ba6f049 100644 --- a/main.nf +++ b/main.nf @@ -46,9 +46,6 @@ workflow NFCORE_RNAFUSION { RNAFUSION() } - emit: - multiqc_report = RNAFUSION.out.multiqc_report // channel: /path/to/multiqc_report.html - } /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -88,7 +85,6 @@ workflow { params.outdir, params.monochrome_logs, params.hook_url, - NFCORE_RNAFUSION.out.multiqc_report ) } diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf index 98b9d7e7..e7d4e72d 100644 --- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf @@ -98,7 +98,6 @@ workflow PIPELINE_COMPLETION { outdir // path: Path to output directory where results will be published monochrome_logs // boolean: Disable ANSI colour codes in log output hook_url // string: hook URL for notifications - multiqc_report // string: Path to MultiQC report main: @@ -109,7 +108,7 @@ workflow PIPELINE_COMPLETION { // workflow.onComplete { if (email || email_on_fail) { - completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, null) } completionSummary(monochrome_logs) From 22e3ecb163e21e6e32c1394bca71931b84e8ae52 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 4 Mar 2024 20:01:02 +0100 Subject: [PATCH 37/64] checks --- conf/modules.config | 4 ---- nextflow.config | 2 -- workflows/build_references.nf | 13 ------------- workflows/rnafusion.nf | 6 ++---- 4 files changed, 2 insertions(+), 23 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 5bd32d63..9d4ca62b 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -18,10 +18,6 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: FASTQC { - ext.args = '--quiet' - } - withName: 'ARRIBA' { publishDir = [ path: { "${params.outdir}/arriba" }, diff --git a/nextflow.config b/nextflow.config index 3c22cd82..1a35e3c7 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,8 +16,6 @@ params { cosmic_passwd = null qiagen = false - - // MultiQC options multiqc_config = null multiqc_title = null diff --git a/workflows/build_references.nf b/workflows/build_references.nf index cfbafafe..7847dd05 100644 --- a/workflows/build_references.nf +++ b/workflows/build_references.nf @@ -80,19 +80,6 @@ workflow BUILD_REFERENCES { } -/* -======================================================================================== - COMPLETION EMAIL AND SUMMARY -======================================================================================== -*/ - -// workflow.onComplete { -// if (params.email || params.email_on_fail) { -// NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) -// } -// NfcoreTemplate.summary(workflow, params, log) -// } - /* ======================================================================================== THE END diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf index 98345287..63c2f1f1 100644 --- a/workflows/rnafusion.nf +++ b/workflows/rnafusion.nf @@ -14,7 +14,7 @@ include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' -// // Check mandatory parameters +// Check mandatory parameters // //TODO: move this to utils_nf_core_rnafusion_pipeline @@ -98,8 +98,6 @@ include { CAT_FASTQ } from '../modules/nf-core/cat/fastq/main' workflow RNAFUSION { - main: - ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() @@ -147,7 +145,7 @@ workflow RNAFUSION { ch_cat_fastq ) ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) - ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + ch_versions = ch_versions.mix(FASTQC.out.versions) TRIM_WORKFLOW ( ch_cat_fastq From dae6bd1c5f5df9d108c6ad405008bfcc195ed309 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Wed, 6 Mar 2024 09:00:48 +0100 Subject: [PATCH 38/64] remove commented out param checks, moved to issue --- workflows/rnafusion.nf | 30 ------------------------------ 1 file changed, 30 deletions(-) diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf index 63c2f1f1..a1b8f431 100644 --- a/workflows/rnafusion.nf +++ b/workflows/rnafusion.nf @@ -11,18 +11,6 @@ include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pi include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_rnafusion_pipeline' include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' - - - -// Check mandatory parameters - - -// //TODO: move this to utils_nf_core_rnafusion_pipeline -// if (file(params.input).exists() || params.build_references) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet does not exist or was not specified!' } -// if (params.fusioninspector_only && !params.fusioninspector_fusions) { exit 1, 'Parameter --fusioninspector_fusions PATH_TO_FUSION_LIST expected with parameter --fusioninspector_only'} -// if (params.tools_cutoff < 1) { exit 1, 'Parameter: --tools_cutoff should be >= 1'} - - ch_chrgtf = params.starfusion_build ? Channel.fromPath(params.chrgtf).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_annot.gtf").map { it -> [[id:it.Name], it] }.collect() ch_starindex_ref = params.starfusion_build ? Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect() : Channel.fromPath("${params.starfusion_ref}/ref_genome.fa.star.idx").map { it -> [[id:it.Name], it] }.collect() ch_starindex_ensembl_ref = Channel.fromPath(params.starindex_ref).map { it -> [[id:it.Name], it] }.collect() @@ -40,24 +28,6 @@ ch_gtf = Channel.fromPath(params.gtf).map { it -> [[id:it.Name], it] }.collect() ch_transcript = Channel.fromPath(params.transcript).map { it -> [[id:it.Name], it] }.collect() ch_fai = Channel.fromPath(params.fai).map { it -> [[id:it.Name], it] }.collect() -// def checkPathParamList = [ -// params.fasta, -// params.fai, -// params.gtf, -// params.transcript, -// ] - -// for (param in checkPathParamList) if ((param) && !params.build_references) file(param, checkIfExists: true) -// def params_fasta_path_uri = params.fasta =~ /^([a-zA-Z0-9]*):\/\/(?:[-a-zA-Z0-9()@:%_\+.~#?&\/=]*)$/ -// // check if params.fasta is a remote uri such as s3://, gs:// or dx:// -// if (params_fasta_path_uri){ -// log.info "INFO: a remote uri path detected, check for absolute path and trailing '/' not performed" -// // log.info "INFO: remote uri path detected (e.g. s3), check for absolute path and trailing '/' not performed" -// } -// else { -// for (param in checkPathParamList) if ((param.toString())!= file(param).toString() && !params.build_references) { exit 1, "Problem with ${param}: ABSOLUTE PATHS are required! Check for trailing '/' at the end of paths too." } -// } - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From bddbb710becadaa4d0e64e552328acc1fbd432da Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 8 Mar 2024 13:51:11 +0100 Subject: [PATCH 39/64] add strandedness in input schema --- .nf-test.log | 4 ++++ assets/schema_input.json | 9 ++++++++- nf-test.config | 8 ++++++++ tests/main.nf.test | 31 +++++++++++++++++++++++++++++++ 4 files changed, 51 insertions(+), 1 deletion(-) create mode 100644 .nf-test.log create mode 100644 nf-test.config create mode 100644 tests/main.nf.test diff --git a/.nf-test.log b/.nf-test.log new file mode 100644 index 00000000..8da6769a --- /dev/null +++ b/.nf-test.log @@ -0,0 +1,4 @@ +Mar-06 08:33:23.861 [main] INFO com.askimed.nf.test.App - nf-test 0.8.1 +Mar-06 08:33:23.919 [main] INFO com.askimed.nf.test.App - Arguments: [test, --profile, debug,test,docker, --verbose] +Mar-06 08:33:23.924 [main] INFO com.askimed.nf.test.commands.RunTestsCommand - Load config from file /Users/annick.renevey/Projects/rnafusion/nf-test.config... +Mar-06 08:33:24.984 [main] ERROR com.askimed.nf.test.commands.RunTestsCommand - No tests ot directories found containing test files. diff --git a/assets/schema_input.json b/assets/schema_input.json index fa630b6c..12ec2cc8 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -26,8 +26,15 @@ "exists": true, "pattern": "^\\S+\\.f(ast)?q\\.gz$", "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" + }, + "strandedness": { + "type": "string", + "format": "string", + "exists": true, + "pattern": "forward|reverse|unstranded|unknown", + "errorMessage": "Strandedness has to be forward, reverse, unstranded or unknown" } }, - "required": ["sample", "fastq_1"] + "required": ["sample", "fastq_1", "fastq_2", "strandedness"] } } diff --git a/nf-test.config b/nf-test.config new file mode 100644 index 00000000..870799df --- /dev/null +++ b/nf-test.config @@ -0,0 +1,8 @@ +config { + + testsDir "tests" + workDir ".nf-test" + configFile "tests/nextflow.config" + profile "" + +} diff --git a/tests/main.nf.test b/tests/main.nf.test new file mode 100644 index 00000000..25f5c9aa --- /dev/null +++ b/tests/main.nf.test @@ -0,0 +1,31 @@ +nextflow_pipeline { + + name "Test pipeline" + script "../main.nf" + tag "pipeline" + tag "pipeline_rnafusion" + + test("Run build references with profile test") { + + when { + params { + build_references = true + outdir = "results" + genome_base = "references" + max_cpus = 2 + max_memory = '6.GB' + max_time = '6.h' + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv' + cosmic_username = secrets.COSMIC_USERNAME + cosmic_passwd = secrets.COSMIC_PASSWD + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + +} From 2c1f1e5c91ace13c2b0448086e627ae8061839a7 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 8 Mar 2024 14:14:48 +0100 Subject: [PATCH 40/64] update instructions to run nf-test --- .github/CONTRIBUTING.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 8e5d1fef..3cfa08b3 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -26,7 +26,7 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests -You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command: +You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command, BUT DON'T FORGET TO ADD THE PARAMETERS cosmic_username AND cosmic_passwd in tests/main.nf.test. ```bash nf-test test --profile debug,test,docker --verbose From 255d91ca95acf9785c6b30f680faee7eef3b4160 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 8 Mar 2024 14:15:02 +0100 Subject: [PATCH 41/64] writh pipeline test --- tests/main.nf.test | 22 ++++++++++++++++++++-- 1 file changed, 20 insertions(+), 2 deletions(-) diff --git a/tests/main.nf.test b/tests/main.nf.test index 25f5c9aa..715335d8 100644 --- a/tests/main.nf.test +++ b/tests/main.nf.test @@ -16,8 +16,8 @@ nextflow_pipeline { max_memory = '6.GB' max_time = '6.h' input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv' - cosmic_username = secrets.COSMIC_USERNAME - cosmic_passwd = secrets.COSMIC_PASSWD + cosmic_username = COSMIC_USERNAME + cosmic_passwd = COSMIC_PASSWD } } @@ -27,5 +27,23 @@ nextflow_pipeline { ) } } + test("Run fusion detection with profile test") { + when { + params { + outdir = "results" + genome_base = "references" + max_cpus = 2 + max_memory = '6.GB' + max_time = '6.h' + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv' + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } } From ffed4c05c54ec05345a4b1b8cb0506f7aa05de4b Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 8 Mar 2024 14:15:25 +0100 Subject: [PATCH 42/64] write general test config --- tests/nextflow.config | 35 +++++++++++++++++++++++++++++++++++ 1 file changed, 35 insertions(+) create mode 100644 tests/nextflow.config diff --git a/tests/nextflow.config b/tests/nextflow.config new file mode 100644 index 00000000..ebd067ac --- /dev/null +++ b/tests/nextflow.config @@ -0,0 +1,35 @@ +/* +======================================================================================== + Nextflow config file for running tests +======================================================================================== +*/ + +params { + // Base directory for nf-core/modules test data + modules_testdata_base_path = 's3://ngi-igenomes/testdata/nf-core/modules/' + + // Base directory for nf-core/rnaseq test data + pipelines_testdata_base_path = 's3://ngi-igenomes/testdata/nf-core/pipelines/rnaseq/3.15/' + + outdir = 'results' +} + +// Impose sensible resource limits for testing +process { + withName: '.*' { + cpus = 2 + memory = 3.GB + time = 2.h + } +} + +// Impose same minimum Nextflow version as the pipeline for testing +manifest { + nextflowVersion = '!>=23.04.0' +} + +// Disable all Nextflow reporting options +timeline { enabled = false } +report { enabled = false } +trace { enabled = false } +dag { enabled = false } From 645634c3fc178bb15766502e073c37a8b4de6147 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 8 Mar 2024 14:18:42 +0100 Subject: [PATCH 43/64] comment out nf-test from PR template --- .github/PULL_REQUEST_TEMPLATE.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 8b85a0aa..d2824258 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/rnaf - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/rnafusion/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/rnafusion _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). + - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. From 6a31fd0d49d48dffe5e9f9cdca3728478cf618da Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 8 Mar 2024 14:56:57 +0100 Subject: [PATCH 44/64] CONTRIBUTING AND PULL_REQUEST_TEMPLATE not part of files_unchanged --- .nf-core.yml | 4 + .../meta/assets | 1 + .../818076bdee20d7e7df39ccd4d6617997/meta/bin | 1 + .../meta/nextflow.log | 314 ++++++++++++++++++ .../meta/params.json | 1 + .../meta/std.err | 0 .../meta/std.out | 145 ++++++++ .../meta/trace.csv | 3 + 8 files changed, 469 insertions(+) create mode 120000 .nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/assets create mode 120000 .nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/bin create mode 100644 .nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/nextflow.log create mode 100644 .nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/params.json create mode 100644 .nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/std.err create mode 100644 .nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/std.out create mode 100644 .nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/trace.csv diff --git a/.nf-core.yml b/.nf-core.yml index 3805dc81..78a3fd2e 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1 +1,5 @@ repository_type: pipeline +lint: + files_unchanged: + - .github/CONTRIBUTING.md + - .github/PULL_REQUEST_TEMPLATE.md diff --git a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/assets b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/assets new file mode 120000 index 00000000..e4ea7900 --- /dev/null +++ b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/assets @@ -0,0 +1 @@ +/Users/annick.renevey/Projects/rnafusion/assets \ No newline at end of file diff --git a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/bin b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/bin new file mode 120000 index 00000000..db857c9f --- /dev/null +++ b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/bin @@ -0,0 +1 @@ +/Users/annick.renevey/Projects/rnafusion/bin \ No newline at end of file diff --git a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/nextflow.log b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/nextflow.log new file mode 100644 index 00000000..586050f1 --- /dev/null +++ b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/nextflow.log @@ -0,0 +1,314 @@ +Mar-08 14:13:11.080 [main] DEBUG nextflow.cli.Launcher - $> nextflow -log /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/nextflow.log run /Users/annick.renevey/Projects/rnafusion/tests/../main.nf -c /Users/annick.renevey/Projects/rnafusion/nextflow.config -c /Users/annick.renevey/Projects/rnafusion/tests/nextflow.config -params-file /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/params.json -ansi-log false -profile debug,test,docker -with-trace /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/trace.csv -w /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/work +Mar-08 14:13:11.190 [main] INFO nextflow.cli.CmdRun - N E X T F L O W ~ version 23.04.1 +Mar-08 14:13:11.228 [main] DEBUG nextflow.plugin.PluginsFacade - Setting up plugin manager > mode=prod; embedded=false; plugins-dir=/Users/annick.renevey/.nextflow/plugins; core-plugins: nf-amazon@1.16.2,nf-azure@1.0.1,nf-codecommit@0.1.4,nf-console@1.0.5,nf-ga4gh@1.0.5,nf-google@1.7.3,nf-tower@1.5.12,nf-wave@0.8.2 +Mar-08 14:13:11.245 [main] INFO org.pf4j.DefaultPluginStatusProvider - Enabled plugins: [] +Mar-08 14:13:11.246 [main] INFO org.pf4j.DefaultPluginStatusProvider - Disabled plugins: [] +Mar-08 14:13:11.250 [main] INFO org.pf4j.DefaultPluginManager - PF4J version 3.4.1 in 'deployment' mode +Mar-08 14:13:11.269 [main] INFO org.pf4j.AbstractPluginManager - No plugins +Mar-08 14:13:11.303 [main] DEBUG nextflow.config.ConfigBuilder - Found config base: /Users/annick.renevey/Projects/rnafusion/nextflow.config +Mar-08 14:13:11.310 [main] DEBUG nextflow.config.ConfigBuilder - User config file: /Users/annick.renevey/Projects/rnafusion/nextflow.config +Mar-08 14:13:11.311 [main] DEBUG nextflow.config.ConfigBuilder - User config file: /Users/annick.renevey/Projects/rnafusion/tests/nextflow.config +Mar-08 14:13:11.311 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /Users/annick.renevey/Projects/rnafusion/nextflow.config +Mar-08 14:13:11.311 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /Users/annick.renevey/Projects/rnafusion/nextflow.config +Mar-08 14:13:11.311 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /Users/annick.renevey/Projects/rnafusion/tests/nextflow.config +Mar-08 14:13:11.393 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `debug,test,docker` +Mar-08 14:13:16.099 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `debug,test,docker` +Mar-08 14:13:17.277 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `debug,test,docker` +Mar-08 14:13:17.413 [main] DEBUG nextflow.config.ConfigBuilder - Available config profiles: [cfc_dev, uzl_omics, ifb_core, denbi_qbic, alice, mjolnir_globe, uppmax, incliva, ilifu, uge, rosalind_uge, lugh, unibe_ibu, vai, czbiohub_aws, jax, ccga_med, scw, unc_longleaf, tigem, tubingen_apg, google, apollo, ipop_up, vsc_calcua, pdc_kth, googlels, daisybio, eddie, medair, biowulf, apptainer, bi, bigpurple, adcra, cedars, pawsey_setonix, vsc_kul_uhasselt, pawsey_nimbus, ucl_myriad, utd_ganymede, charliecloud, icr_davros, ceres, munin, arm, rosalind, hasta, cfc, uzh, ebi_codon_slurm, ebc, ku_sund_dangpu, ccga_dx, crick, marvin, biohpc_gen, shifter, mana, mamba, york_viking, unc_lccc, wehi, awsbatch, wustl_htcf, imperial, maestro, software_license, utd_europa, genotoul, nci_gadi, abims, janelia, nu_genomics, googlebatch, oist, sahmri, mpcdf, leicester, vsc_ugent, create, sage, cambridge, jex, podman, ebi_codon, cheaha, xanadu, nyu_hpc, test, computerome, ucd_sonic, seg_globe, sanger, dkfz, pasteur, ethz_euler, test_full, imb, tuos_stanage, azurebatch, hki, crukmi, csiro_petrichor, docker, engaging, gis, hypatia, psmn, eva, nygc, fgcz, conda, crg, singularity, self_hosted_runner, tufts, uw_hyak_pedslabs, utd_sysbio, debug, genouest, cbe, phoenix, gitpod, seawulf, uod_hpc, fub_curta, uct_hpc, aws_tower, binac] +Mar-08 14:13:17.512 [main] DEBUG nextflow.cli.CmdRun - Applied DSL=2 from script declararion +Mar-08 14:13:17.534 [main] INFO nextflow.cli.CmdRun - Launching `/Users/annick.renevey/Projects/rnafusion/tests/../main.nf` [cheesy_hawking] DSL2 - revision: 12cef51b71 +Mar-08 14:13:17.536 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins declared=[nf-validation@1.1.3] +Mar-08 14:13:17.541 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins default=[] +Mar-08 14:13:17.541 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins resolved requirement=[nf-validation@1.1.3] +Mar-08 14:13:17.542 [main] DEBUG nextflow.plugin.PluginUpdater - Installing plugin nf-validation version: 1.1.3 +Mar-08 14:13:17.559 [main] INFO org.pf4j.AbstractPluginManager - Plugin 'nf-validation@1.1.3' resolved +Mar-08 14:13:17.560 [main] INFO org.pf4j.AbstractPluginManager - Start plugin 'nf-validation@1.1.3' +Mar-08 14:13:17.592 [main] DEBUG nextflow.plugin.BasePlugin - Plugin started nf-validation@1.1.3 +Mar-08 14:13:17.616 [main] DEBUG nextflow.secret.LocalSecretsProvider - Secrets store: /Users/annick.renevey/.nextflow/secrets/store.json +Mar-08 14:13:17.628 [main] DEBUG nextflow.secret.SecretsLoader - Discovered secrets providers: [nextflow.secret.LocalSecretsProvider@57e03347] - activable => nextflow.secret.LocalSecretsProvider@57e03347 +Mar-08 14:13:17.713 [main] DEBUG nextflow.Session - Session UUID: 1bbe00d2-1928-4556-b97c-ecef4af4222e +Mar-08 14:13:17.713 [main] DEBUG nextflow.Session - Run name: cheesy_hawking +Mar-08 14:13:17.714 [main] DEBUG nextflow.Session - Executor pool size: 8 +Mar-08 14:13:17.727 [main] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'FileTransfer' minSize=10; maxSize=24; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Mar-08 14:13:17.787 [main] DEBUG nextflow.cli.CmdRun - + Version: 23.04.1 build 5866 + Created: 15-04-2023 06:51 UTC (08:51 CEST) + System: Mac OS X 14.2.1 + Runtime: Groovy 3.0.16 on OpenJDK 64-Bit Server VM 17.0.3+7-LTS + Encoding: UTF-8 (UTF-8) + Process: 89543@annickrmbp [130.229.140.244] + CPUs: 8 - Mem: 16 GB (22.8 MB) - Swap: 6 GB (1.4 GB) +Mar-08 14:13:17.818 [main] DEBUG nextflow.Session - Work-dir: /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/work [Mac OS X] +Mar-08 14:13:17.840 [main] DEBUG nextflow.executor.ExecutorFactory - Extension executors providers=[] +Mar-08 14:13:17.857 [main] DEBUG nextflow.Session - Observer factory: DefaultObserverFactory +Mar-08 14:13:17.959 [main] DEBUG nextflow.cache.CacheFactory - Using Nextflow cache factory: nextflow.cache.DefaultCacheFactory +Mar-08 14:13:17.990 [main] DEBUG nextflow.util.CustomThreadPool - Creating default thread pool > poolSize: 9; maxThreads: 1000 +Mar-08 14:13:18.140 [main] DEBUG nextflow.Session - Session start +Mar-08 14:13:18.175 [main] DEBUG nextflow.trace.TraceFileObserver - Workflow started -- trace file: /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/trace.csv +Mar-08 14:13:18.579 [main] DEBUG nextflow.script.ScriptRunner - > Launching execution +Mar-08 14:13:19.365 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [paramsHelp:paramsHelp]; plugin Id: nf-validation +Mar-08 14:13:19.385 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [paramsSummaryLog:paramsSummaryLog]; plugin Id: nf-validation +Mar-08 14:13:19.385 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [validateParameters:validateParameters]; plugin Id: nf-validation +Mar-08 14:13:19.411 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [paramsSummaryMap:paramsSummaryMap]; plugin Id: nf-validation +Mar-08 14:13:19.413 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [fromSamplesheet:fromSamplesheet]; plugin Id: nf-validation +Mar-08 14:13:22.583 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [paramsHelp:paramsHelp]; plugin Id: nf-validation +Mar-08 14:13:22.586 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [paramsSummaryLog:paramsSummaryLog]; plugin Id: nf-validation +Mar-08 14:13:22.586 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [validateParameters:validateParameters]; plugin Id: nf-validation +Mar-08 14:13:22.588 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [paramsSummaryMap:paramsSummaryMap]; plugin Id: nf-validation +Mar-08 14:13:22.588 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [fromSamplesheet:fromSamplesheet]; plugin Id: nf-validation +Mar-08 14:13:22.738 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [paramsSummaryLog:paramsSummaryLog, paramsSummaryMap:paramsSummaryMap]; plugin Id: nf-validation +Mar-08 14:13:27.381 [main] DEBUG nextflow.Nextflow - Using schema file: nextflow_schema.json +Mar-08 14:13:27.389 [main] WARN nextflow.script.ScriptBinding - Access to undefined parameter `monochromeLogs` -- Initialise it to a default value eg. `params.monochromeLogs = some_value` +Mar-08 14:13:27.418 [main] INFO nextflow.Nextflow - + +------------------------------------------------------ + ,--./,-. + ___ __ __ __ ___ /,-._.--~' + |\ | |__ __ / ` / \ |__) |__ } { + | \| | \__, \__/ | \ |___ \`-._,-`-, + `._,._,' + nf-core/rnafusion v3.0.2dev +------------------------------------------------------ +Core Nextflow options + runName : cheesy_hawking + containerEngine : docker + launchDir : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997 + workDir : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/work + projectDir : /Users/annick.renevey/Projects/rnafusion + userName : annick.renevey + profile : debug,test,docker + configFiles :  + +Input/output options + input : https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv + outdir : results + build_references : true + genomes_base : results/references + starfusion_build : true + arriba_ref : results/references/arriba + arriba_ref_blacklist : results/references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz + arriba_ref_cytobands : results/references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv + arriba_ref_known_fusions : results/references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz + arriba_ref_protein_domains : results/references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3 + ensembl_ref : results/references/ensembl + fusioncatcher_limitSjdbInsertNsj : 2000000 + fusioncatcher_ref : results/references/fusioncatcher/human_v102 + fusioninspector_limitSjdbInsertNsj: 1000000 + fusionreport_ref : results/references/fusion_report_db + hgnc_ref : results/references/hgnc/hgnc_complete_set.txt + hgnc_date : results/references/hgnc/HGNC-DB-timestamp.txt + starfusion_ref : results/references/starfusion/ctat_genome_lib_build_dir + starindex_ref : results/references/star + tools_cutoff : 1 + +Read trimming options + adapter_fasta : [] + +Alignment compression options + cram : [] + +Reference genome options + genome : GRCh38 + fasta : results/references/ensembl/Homo_sapiens.GRCh38.102.all.fa + fai : results/references/ensembl/Homo_sapiens.GRCh38.102.all.fa.fai + gtf : results/references/ensembl/Homo_sapiens.GRCh38.102.gtf + chrgtf : results/references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf + transcript : results/references/ensembl/Homo_sapiens.GRCh38.102.cdna.all.fa.gz + refflat : results/references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf.refflat + rrna_intervals : results/references/ensembl/Homo_sapiens.GRCh38.102.interval_list + +Institutional config options + config_profile_name : Test profile + config_profile_description : Minimal test dataset to check pipeline function + +Max job request options + max_cpus : 2 + max_memory : 6.GB + max_time : 6.h + +!! Only displaying parameters that differ from the pipeline defaults !! +------------------------------------------------------ +If you use nf-core/rnafusion for your analysis please cite: + +* The pipeline + + +* The nf-core framework + https://doi.org/10.1038/s41587-020-0439-x + +* Software dependencies + https://github.com/nf-core/rnafusion/blob/master/CITATIONS.md +------------------------------------------------------ +Mar-08 14:13:27.425 [main] DEBUG nextflow.validation.SchemaValidator - Starting parameters validation +Mar-08 14:13:27.617 [main] WARN nextflow.validation.SchemaValidator - The following invalid input values have been detected: + +* --genome_base: references +* --modules_testdata_base_path: s3://ngi-igenomes/testdata/nf-core/modules/ +* --pipelines_testdata_base_path: s3://ngi-igenomes/testdata/nf-core/pipelines/rnaseq/3.15/ + + +Mar-08 14:13:27.912 [main] DEBUG nextflow.validation.SchemaValidator - Starting validation: 'input': '/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv' with '/Users/annick.renevey/Projects/rnafusion/assets/schema_input.json' +Mar-08 14:13:29.935 [main] DEBUG nextflow.validation.SchemaValidator - Validation passed: 'input': '/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv' with '/Users/annick.renevey/Projects/rnafusion/assets/schema_input.json' +Mar-08 14:13:29.935 [main] DEBUG nextflow.validation.SchemaValidator - Finishing parameters validation +Mar-08 14:13:30.046 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD +Mar-08 14:13:30.049 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:ENSEMBL_DOWNLOAD` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD +Mar-08 14:13:30.050 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD +Mar-08 14:13:30.053 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD +Mar-08 14:13:30.061 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null +Mar-08 14:13:30.061 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' +Mar-08 14:13:30.083 [main] DEBUG nextflow.executor.Executor - [warm up] executor > local +Mar-08 14:13:30.090 [main] DEBUG n.processor.LocalPollingMonitor - Creating local task monitor for executor 'local' > cpus=8; memory=16 GB; capacity=8; pollInterval=100ms; dumpInterval=5m +Mar-08 14:13:30.203 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD +Mar-08 14:13:30.204 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:HGNC_DOWNLOAD` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD +Mar-08 14:13:30.206 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD +Mar-08 14:13:30.207 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD +Mar-08 14:13:30.213 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null +Mar-08 14:13:30.214 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' +Mar-08 14:13:30.236 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_single` matches labels `process_single` for process with name NFCORE_RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX +Mar-08 14:13:30.237 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:SAMTOOLS_FAIDX` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX +Mar-08 14:13:30.238 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX +Mar-08 14:13:30.238 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX +Mar-08 14:13:30.240 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null +Mar-08 14:13:30.240 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' +Mar-08 14:13:30.248 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_CREATESEQUENCEDICTIONARY +Mar-08 14:13:30.248 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_CREATESEQUENCEDICTIONARY +Mar-08 14:13:30.249 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_CREATESEQUENCEDICTIONARY +Mar-08 14:13:30.250 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null +Mar-08 14:13:30.250 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' +Mar-08 14:13:30.263 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_single` matches labels `process_single` for process with name NFCORE_RNAFUSION:BUILD_REFERENCES:RRNA_TRANSCRIPTS +Mar-08 14:13:30.264 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:RRNA_TRANSCRIPTS +Mar-08 14:13:30.264 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:RRNA_TRANSCRIPTS +Mar-08 14:13:30.265 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null +Mar-08 14:13:30.265 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' +Mar-08 14:13:30.273 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_single` matches labels `process_single` for process with name NFCORE_RNAFUSION:BUILD_REFERENCES:CONVERT2BED +Mar-08 14:13:30.274 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:CONVERT2BED +Mar-08 14:13:30.274 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:CONVERT2BED +Mar-08 14:13:30.274 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null +Mar-08 14:13:30.275 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' +Mar-08 14:13:30.290 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST +Mar-08 14:13:30.292 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:GATK4_BEDTOINTERVALLIST` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST +Mar-08 14:13:30.292 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST +Mar-08 14:13:30.292 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST +Mar-08 14:13:30.294 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null +Mar-08 14:13:30.294 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' +Mar-08 14:13:30.302 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT +Mar-08 14:13:30.303 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:GTF_TO_REFFLAT` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT +Mar-08 14:13:30.304 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT +Mar-08 14:13:30.304 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT +Mar-08 14:13:30.306 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null +Mar-08 14:13:30.306 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' +Mar-08 14:13:30.322 [main] DEBUG nextflow.Session - Workflow process names [dsl2]: SAMTOOLS_VIEW_FOR_STARFUSION, SAMTOOLS_SORT_FOR_ARRIBA, GATK4_BEDTOINTERVALLIST, STAR_FOR_ARRIBA, STARFUSION_DOWNLOAD, FUSIONCATCHER, FUSIONCATCHER_DOWNLOAD, STAR_FOR_STARFUSION, GATK4_CREATESEQUENCEDICTIONARY, FASTP, FASTQC_FOR_FASTP, ENSEMBL_DOWNLOAD, GATK4_MARKDUPLICATES, CAT_FASTQ, GTF_TO_REFFLAT, STARFUSION, NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST, NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_CREATESEQUENCEDICTIONARY, NFCORE_RNAFUSION:BUILD_REFERENCES:CONVERT2BED, VCF_COLLECT, SAMTOOLS_INDEX_FOR_ARRIBA, ARRIBA_VISUALISATION, CONVERT2BED, FUSIONREPORT_DOWNLOAD, STAR_ALIGN, SAMTOOLS_INDEX_FOR_STARFUSION, FUSIONINSPECTOR, SAMTOOLS_INDEX, FASTQC, ARRIBA_DOWNLOAD, NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD, MULTIQC, NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD, STRINGTIE_MERGE, SAMTOOLS_SORT, SAMTOOLS_INDEX_FOR_STARFUSION_CRAM, SAMTOOLS_VIEW_FOR_ARRIBA, NFCORE_RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT, NFCORE_RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX, STARFUSION_BUILD, CAT_CAT, PICARD_COLLECTRNASEQMETRICS, HGNC_DOWNLOAD, SAMTOOLS_VIEW, SAMTOOLS_FAIDX, FUSIONREPORT, RRNA_TRANSCRIPTS, AGAT_CONVERTSPGFF2TSV, STRINGTIE_STRINGTIE, PICARD_COLLECTINSERTSIZEMETRICS, ARRIBA, NFCORE_RNAFUSION:BUILD_REFERENCES:RRNA_TRANSCRIPTS, STAR_GENOMEGENERATE +Mar-08 14:13:30.337 [main] WARN nextflow.Session - There's no process matching config selector: SAMPLESHEET_CHECK +Mar-08 14:13:30.337 [main] DEBUG nextflow.Session - Igniting dataflow network (25) +Mar-08 14:13:30.369 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD +Mar-08 14:13:30.370 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD +Mar-08 14:13:30.371 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX +Mar-08 14:13:30.371 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_CREATESEQUENCEDICTIONARY +Mar-08 14:13:30.372 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNAFUSION:BUILD_REFERENCES:RRNA_TRANSCRIPTS +Mar-08 14:13:30.372 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNAFUSION:BUILD_REFERENCES:CONVERT2BED +Mar-08 14:13:30.373 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST +Mar-08 14:13:30.373 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT +Mar-08 14:13:30.373 [main] DEBUG nextflow.script.ScriptRunner - > Awaiting termination +Mar-08 14:13:30.373 [main] DEBUG nextflow.Session - Session await +Mar-08 14:13:30.553 [Actor Thread 17] INFO nextflow.processor.TaskProcessor - [NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD] cache hash: 7f18070817f5b2ac14c8e745abfb6933; mode: STANDARD; entries: + 338337db5eff3bff24c213e2d8964e63 [java.util.UUID] 1bbe00d2-1928-4556-b97c-ecef4af4222e + 5dd471f5f4c9a54dea64867d4265ee28 [java.lang.String] NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD + 9ff7290eb13332bf5c4063dc712689d6 [java.lang.String] """ + wget https://ftp.ebi.ac.uk/pub/databases/genenames/hgnc/tsv/hgnc_complete_set.txt + date +%Y-%m-%d/%H:%M > HGNC-DB-timestamp.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml) + END_VERSIONS + """ + + 00c7c646a986ea5502fb404ea15e7443 [java.lang.String] quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5 + 4f9d4b0d22865056c37fb6d9c2a04a67 [java.lang.String] $ + 16fe7483905cce7a85670e43e4678877 [java.lang.Boolean] true + 77476d335f7c3b5b95495d42532f58d0 [java.util.HashMap$EntrySet] [task.process=NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD] + +Mar-08 14:13:30.613 [Actor Thread 15] INFO nextflow.processor.TaskProcessor - [NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD] cache hash: dd31277bda420c7e0145fc89ea8613e5; mode: STANDARD; entries: + 338337db5eff3bff24c213e2d8964e63 [java.util.UUID] 1bbe00d2-1928-4556-b97c-ecef4af4222e + f65e11932b552ca585de284c5e5d0162 [java.lang.String] NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD + 0955cd72953c2b84dffe718d8e97dc15 [java.lang.String] """ + wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{1..22}.fa.gz + wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{MT,X,Y}.fa.gz + + wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz + wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz + wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz + + gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}.${ensembl_version}.all.fa + gunzip Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz + gunzip Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz + + + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml) + END_VERSIONS + """ + + 00c7c646a986ea5502fb404ea15e7443 [java.lang.String] quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5 + fee0e11be1152749b19f3d19542d3901 [java.lang.String] ensembl_version + f56f0c2893db292d86b93804fe80b6ac [java.lang.Integer] 102 + d9c152f5939e0f93a25dbd62b7c4d0a5 [java.lang.String] genome + 6b15613e5b490261f4ffe786a92c5825 [java.lang.String] GRCh38 + 22a9e5e0e97f0475e310f04c56b1393a [java.lang.String] meta + cb0f105a57d4145f7acb92ca9cf2ea12 [java.util.LinkedHashMap] [id:Homo_sapiens.GRCh38.102] + 4f9d4b0d22865056c37fb6d9c2a04a67 [java.lang.String] $ + 16fe7483905cce7a85670e43e4678877 [java.lang.Boolean] true + c877f2c88b6ad8c3dbe3236a80f57755 [java.util.HashMap$EntrySet] [task.process=NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD, params.genome=GRCh38] + +Mar-08 14:13:30.754 [Task submitter] DEBUG n.executor.local.LocalTaskHandler - Launch cmd line: /bin/bash -ue .command.run +Mar-08 14:13:30.756 [Task submitter] INFO nextflow.Session - [81/2c2c1f] Submitted process > NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD (hgnc) +Mar-08 14:13:30.864 [Task submitter] DEBUG n.executor.local.LocalTaskHandler - Launch cmd line: /bin/bash -ue .command.run +Mar-08 14:13:30.865 [Task submitter] INFO nextflow.Session - [52/33e328] Submitted process > NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD (ensembl) +Mar-08 14:14:06.817 [Task monitor] DEBUG n.processor.TaskPollingMonitor - Task completed > TaskHandler[id: 1; name: NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD (hgnc); status: COMPLETED; exit: 0; error: -; workDir: /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/work/81/2c2c1f77cfe71f861687ce1e2e35ba] +Mar-08 14:14:06.983 [Task monitor] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'PublishDir' minSize=10; maxSize=24; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Mar-08 14:14:10.653 [SIGINT handler] DEBUG nextflow.Session - Session aborted -- Cause: SIGINT +Mar-08 14:14:10.717 [SIGINT handler] DEBUG nextflow.Session - The following nodes are still active: +[process] NFCORE_RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX + status=ACTIVE + port 0: (value) OPEN ; channel: - + port 1: (value) bound ; channel: - + port 2: (cntrl) - ; channel: $ + +[process] NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_CREATESEQUENCEDICTIONARY + status=ACTIVE + port 0: (value) OPEN ; channel: - + port 1: (cntrl) - ; channel: $ + +[process] NFCORE_RNAFUSION:BUILD_REFERENCES:RRNA_TRANSCRIPTS + status=ACTIVE + port 0: (value) OPEN ; channel: - + port 1: (cntrl) - ; channel: $ + +[process] NFCORE_RNAFUSION:BUILD_REFERENCES:CONVERT2BED + status=ACTIVE + port 0: (value) OPEN ; channel: - + port 1: (cntrl) - ; channel: $ + +[process] NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST + status=ACTIVE + port 0: (value) OPEN ; channel: - + port 1: (value) OPEN ; channel: - + port 2: (cntrl) - ; channel: $ + +[process] NFCORE_RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT + status=ACTIVE + port 0: (value) OPEN ; channel: - + port 1: (cntrl) - ; channel: $ + +Mar-08 14:14:10.812 [main] DEBUG nextflow.Session - Session await > all processes finished +Mar-08 14:14:10.813 [main] DEBUG nextflow.Session - Session await > all barriers passed +Mar-08 14:14:10.829 [main] INFO nextflow.Nextflow - -[nf-core/rnafusion] Pipeline completed with errors- +Mar-08 14:14:10.840 [main] WARN n.processor.TaskPollingMonitor - Killing running tasks (1) +Mar-08 14:14:10.874 [main] DEBUG nextflow.trace.WorkflowStatsObserver - Workflow completed > WorkflowStats[succeededCount=1; failedCount=0; ignoredCount=0; cachedCount=0; pendingCount=0; submittedCount=0; runningCount=1; retriesCount=0; abortedCount=0; succeedDuration=52s; failedDuration=0ms; cachedDuration=0ms;loadCpus=0; loadMemory=0; peakRunning=2; peakCpus=4; peakMemory=6 GB; ] +Mar-08 14:14:10.875 [main] DEBUG nextflow.trace.TraceFileObserver - Workflow completed -- saving trace file +Mar-08 14:14:10.897 [main] DEBUG nextflow.cache.CacheDB - Closing CacheDB done +Mar-08 14:14:10.897 [main] INFO org.pf4j.AbstractPluginManager - Stop plugin 'nf-validation@1.1.3' +Mar-08 14:14:10.898 [main] DEBUG nextflow.plugin.BasePlugin - Plugin stopped nf-validation +Mar-08 14:14:11.002 [main] DEBUG nextflow.script.ScriptRunner - > Execution complete -- Goodbye diff --git a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/params.json b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/params.json new file mode 100644 index 00000000..eda4f800 --- /dev/null +++ b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/params.json @@ -0,0 +1 @@ +{"input":"https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv","cosmic_username":null,"build_references":true,"genome_base":"references","cosmic_passwd":null,"max_time":"6.h","max_cpus":2,"max_memory":"6.GB","outdir":"results"} \ No newline at end of file diff --git a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/std.err b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/std.err new file mode 100644 index 00000000..e69de29b diff --git a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/std.out b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/std.out new file mode 100644 index 00000000..7b566f86 --- /dev/null +++ b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/std.out @@ -0,0 +1,145 @@ +N E X T F L O W ~ version 23.04.1 +Launching `/Users/annick.renevey/Projects/rnafusion/tests/../main.nf` [cheesy_hawking] DSL2 - revision: 12cef51b71 +WARN: Access to undefined parameter `monochromeLogs` -- Initialise it to a default value eg. `params.monochromeLogs = some_value` + + +------------------------------------------------------ + ,--./,-. + ___ __ __ __ ___ /,-._.--~' + |\ | |__ __ / ` / \ |__) |__ } { + | \| | \__, \__/ | \ |___ \`-._,-`-, + `._,._,' + nf-core/rnafusion v3.0.2dev +------------------------------------------------------ +Core Nextflow options + runName : cheesy_hawking + containerEngine : docker + launchDir : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997 + workDir : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/work + projectDir : /Users/annick.renevey/Projects/rnafusion + userName : annick.renevey + profile : debug,test,docker + configFiles :  + +Input/output options + input : https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv + outdir : results + build_references : true + genomes_base : results/references + starfusion_build : true + arriba_ref : results/references/arriba + arriba_ref_blacklist : results/references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz + arriba_ref_cytobands : results/references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv + arriba_ref_known_fusions : results/references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz + arriba_ref_protein_domains : results/references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3 + ensembl_ref : results/references/ensembl + fusioncatcher_limitSjdbInsertNsj : 2000000 + fusioncatcher_ref : results/references/fusioncatcher/human_v102 + fusioninspector_limitSjdbInsertNsj: 1000000 + fusionreport_ref : results/references/fusion_report_db + hgnc_ref : results/references/hgnc/hgnc_complete_set.txt + hgnc_date : results/references/hgnc/HGNC-DB-timestamp.txt + starfusion_ref : results/references/starfusion/ctat_genome_lib_build_dir + starindex_ref : results/references/star + tools_cutoff : 1 + +Read trimming options + adapter_fasta : [] + +Alignment compression options + cram : [] + +Reference genome options + genome : GRCh38 + fasta : results/references/ensembl/Homo_sapiens.GRCh38.102.all.fa + fai : results/references/ensembl/Homo_sapiens.GRCh38.102.all.fa.fai + gtf : results/references/ensembl/Homo_sapiens.GRCh38.102.gtf + chrgtf : results/references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf + transcript : results/references/ensembl/Homo_sapiens.GRCh38.102.cdna.all.fa.gz + refflat : results/references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf.refflat + rrna_intervals : results/references/ensembl/Homo_sapiens.GRCh38.102.interval_list + +Institutional config options + config_profile_name : Test profile + config_profile_description : Minimal test dataset to check pipeline function + +Max job request options + max_cpus : 2 + max_memory : 6.GB + max_time : 6.h + +!! Only displaying parameters that differ from the pipeline defaults !! +------------------------------------------------------ +If you use nf-core/rnafusion for your analysis please cite: + +* The pipeline + + +* The nf-core framework + https://doi.org/10.1038/s41587-020-0439-x + +* Software dependencies + https://github.com/nf-core/rnafusion/blob/master/CITATIONS.md +------------------------------------------------------ +WARN: The following invalid input values have been detected: + +* --genome_base: references +* --modules_testdata_base_path: s3://ngi-igenomes/testdata/nf-core/modules/ +* --pipelines_testdata_base_path: s3://ngi-igenomes/testdata/nf-core/pipelines/rnaseq/3.15/ + + +WARN: There's no process matching config selector: SAMPLESHEET_CHECK +[NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD] cache hash: 7f18070817f5b2ac14c8e745abfb6933; mode: STANDARD; entries: + 338337db5eff3bff24c213e2d8964e63 [java.util.UUID] 1bbe00d2-1928-4556-b97c-ecef4af4222e + 5dd471f5f4c9a54dea64867d4265ee28 [java.lang.String] NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD + 9ff7290eb13332bf5c4063dc712689d6 [java.lang.String] """ + wget https://ftp.ebi.ac.uk/pub/databases/genenames/hgnc/tsv/hgnc_complete_set.txt + date +%Y-%m-%d/%H:%M > HGNC-DB-timestamp.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml) + END_VERSIONS + """ + + 00c7c646a986ea5502fb404ea15e7443 [java.lang.String] quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5 + 4f9d4b0d22865056c37fb6d9c2a04a67 [java.lang.String] $ + 16fe7483905cce7a85670e43e4678877 [java.lang.Boolean] true + 77476d335f7c3b5b95495d42532f58d0 [java.util.HashMap$EntrySet] [task.process=NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD] + +[NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD] cache hash: dd31277bda420c7e0145fc89ea8613e5; mode: STANDARD; entries: + 338337db5eff3bff24c213e2d8964e63 [java.util.UUID] 1bbe00d2-1928-4556-b97c-ecef4af4222e + f65e11932b552ca585de284c5e5d0162 [java.lang.String] NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD + 0955cd72953c2b84dffe718d8e97dc15 [java.lang.String] """ + wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{1..22}.fa.gz + wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{MT,X,Y}.fa.gz + + wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz + wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz + wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz + + gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}.${ensembl_version}.all.fa + gunzip Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz + gunzip Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz + + + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml) + END_VERSIONS + """ + + 00c7c646a986ea5502fb404ea15e7443 [java.lang.String] quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5 + fee0e11be1152749b19f3d19542d3901 [java.lang.String] ensembl_version + f56f0c2893db292d86b93804fe80b6ac [java.lang.Integer] 102 + d9c152f5939e0f93a25dbd62b7c4d0a5 [java.lang.String] genome + 6b15613e5b490261f4ffe786a92c5825 [java.lang.String] GRCh38 + 22a9e5e0e97f0475e310f04c56b1393a [java.lang.String] meta + cb0f105a57d4145f7acb92ca9cf2ea12 [java.util.LinkedHashMap] [id:Homo_sapiens.GRCh38.102] + 4f9d4b0d22865056c37fb6d9c2a04a67 [java.lang.String] $ + 16fe7483905cce7a85670e43e4678877 [java.lang.Boolean] true + c877f2c88b6ad8c3dbe3236a80f57755 [java.util.HashMap$EntrySet] [task.process=NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD, params.genome=GRCh38] + +[81/2c2c1f] Submitted process > NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD (hgnc) +[52/33e328] Submitted process > NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD (ensembl) diff --git a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/trace.csv b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/trace.csv new file mode 100644 index 00000000..57aed492 --- /dev/null +++ b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/trace.csv @@ -0,0 +1,3 @@ +task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar +1 81/2c2c1f 89776 NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD (hgnc) COMPLETED 0 2024-03-08 14:13:30.756 36s 26s 3.0% 24.8 MB 2.3 GB 16.2 MB 15.8 MB +2 52/33e328 89787 NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD (ensembl) ABORTED - 2024-03-08 14:13:30.864 - - - - - - - From 27ae59d1f6e51cad4041858dcc97c76b04fc7e0e Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 8 Mar 2024 15:08:43 +0100 Subject: [PATCH 45/64] prettier --- .../meta/params.json | 12 +++++++++++- 1 file changed, 11 insertions(+), 1 deletion(-) diff --git a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/params.json b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/params.json index eda4f800..691664e7 100644 --- a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/params.json +++ b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/params.json @@ -1 +1,11 @@ -{"input":"https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv","cosmic_username":null,"build_references":true,"genome_base":"references","cosmic_passwd":null,"max_time":"6.h","max_cpus":2,"max_memory":"6.GB","outdir":"results"} \ No newline at end of file +{ + "input": "https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv", + "cosmic_username": null, + "build_references": true, + "genome_base": "references", + "cosmic_passwd": null, + "max_time": "6.h", + "max_cpus": 2, + "max_memory": "6.GB", + "outdir": "results" +} From c4fb47d53b67ae5aeb1d5e25e06e554e5c5706c0 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 8 Mar 2024 15:10:52 +0100 Subject: [PATCH 46/64] remove tests --- .../meta/assets | 1 - .../818076bdee20d7e7df39ccd4d6617997/meta/bin | 1 - .../meta/nextflow.log | 314 ------------------ .../meta/params.json | 11 - .../meta/std.err | 0 .../meta/std.out | 145 -------- .../meta/trace.csv | 3 - 7 files changed, 475 deletions(-) delete mode 120000 .nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/assets delete mode 120000 .nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/bin delete mode 100644 .nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/nextflow.log delete mode 100644 .nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/params.json delete mode 100644 .nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/std.err delete mode 100644 .nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/std.out delete mode 100644 .nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/trace.csv diff --git a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/assets b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/assets deleted file mode 120000 index e4ea7900..00000000 --- a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/assets +++ /dev/null @@ -1 +0,0 @@ -/Users/annick.renevey/Projects/rnafusion/assets \ No newline at end of file diff --git a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/bin b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/bin deleted file mode 120000 index db857c9f..00000000 --- a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/bin +++ /dev/null @@ -1 +0,0 @@ -/Users/annick.renevey/Projects/rnafusion/bin \ No newline at end of file diff --git a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/nextflow.log b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/nextflow.log deleted file mode 100644 index 586050f1..00000000 --- a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/nextflow.log +++ /dev/null @@ -1,314 +0,0 @@ -Mar-08 14:13:11.080 [main] DEBUG nextflow.cli.Launcher - $> nextflow -log /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/nextflow.log run /Users/annick.renevey/Projects/rnafusion/tests/../main.nf -c /Users/annick.renevey/Projects/rnafusion/nextflow.config -c /Users/annick.renevey/Projects/rnafusion/tests/nextflow.config -params-file /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/params.json -ansi-log false -profile debug,test,docker -with-trace /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/trace.csv -w /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/work -Mar-08 14:13:11.190 [main] INFO nextflow.cli.CmdRun - N E X T F L O W ~ version 23.04.1 -Mar-08 14:13:11.228 [main] DEBUG nextflow.plugin.PluginsFacade - Setting up plugin manager > mode=prod; embedded=false; plugins-dir=/Users/annick.renevey/.nextflow/plugins; core-plugins: nf-amazon@1.16.2,nf-azure@1.0.1,nf-codecommit@0.1.4,nf-console@1.0.5,nf-ga4gh@1.0.5,nf-google@1.7.3,nf-tower@1.5.12,nf-wave@0.8.2 -Mar-08 14:13:11.245 [main] INFO org.pf4j.DefaultPluginStatusProvider - Enabled plugins: [] -Mar-08 14:13:11.246 [main] INFO org.pf4j.DefaultPluginStatusProvider - Disabled plugins: [] -Mar-08 14:13:11.250 [main] INFO org.pf4j.DefaultPluginManager - PF4J version 3.4.1 in 'deployment' mode -Mar-08 14:13:11.269 [main] INFO org.pf4j.AbstractPluginManager - No plugins -Mar-08 14:13:11.303 [main] DEBUG nextflow.config.ConfigBuilder - Found config base: /Users/annick.renevey/Projects/rnafusion/nextflow.config -Mar-08 14:13:11.310 [main] DEBUG nextflow.config.ConfigBuilder - User config file: /Users/annick.renevey/Projects/rnafusion/nextflow.config -Mar-08 14:13:11.311 [main] DEBUG nextflow.config.ConfigBuilder - User config file: /Users/annick.renevey/Projects/rnafusion/tests/nextflow.config -Mar-08 14:13:11.311 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /Users/annick.renevey/Projects/rnafusion/nextflow.config -Mar-08 14:13:11.311 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /Users/annick.renevey/Projects/rnafusion/nextflow.config -Mar-08 14:13:11.311 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /Users/annick.renevey/Projects/rnafusion/tests/nextflow.config -Mar-08 14:13:11.393 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `debug,test,docker` -Mar-08 14:13:16.099 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `debug,test,docker` -Mar-08 14:13:17.277 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `debug,test,docker` -Mar-08 14:13:17.413 [main] DEBUG nextflow.config.ConfigBuilder - Available config profiles: [cfc_dev, uzl_omics, ifb_core, denbi_qbic, alice, mjolnir_globe, uppmax, incliva, ilifu, uge, rosalind_uge, lugh, unibe_ibu, vai, czbiohub_aws, jax, ccga_med, scw, unc_longleaf, tigem, tubingen_apg, google, apollo, ipop_up, vsc_calcua, pdc_kth, googlels, daisybio, eddie, medair, biowulf, apptainer, bi, bigpurple, adcra, cedars, pawsey_setonix, vsc_kul_uhasselt, pawsey_nimbus, ucl_myriad, utd_ganymede, charliecloud, icr_davros, ceres, munin, arm, rosalind, hasta, cfc, uzh, ebi_codon_slurm, ebc, ku_sund_dangpu, ccga_dx, crick, marvin, biohpc_gen, shifter, mana, mamba, york_viking, unc_lccc, wehi, awsbatch, wustl_htcf, imperial, maestro, software_license, utd_europa, genotoul, nci_gadi, abims, janelia, nu_genomics, googlebatch, oist, sahmri, mpcdf, leicester, vsc_ugent, create, sage, cambridge, jex, podman, ebi_codon, cheaha, xanadu, nyu_hpc, test, computerome, ucd_sonic, seg_globe, sanger, dkfz, pasteur, ethz_euler, test_full, imb, tuos_stanage, azurebatch, hki, crukmi, csiro_petrichor, docker, engaging, gis, hypatia, psmn, eva, nygc, fgcz, conda, crg, singularity, self_hosted_runner, tufts, uw_hyak_pedslabs, utd_sysbio, debug, genouest, cbe, phoenix, gitpod, seawulf, uod_hpc, fub_curta, uct_hpc, aws_tower, binac] -Mar-08 14:13:17.512 [main] DEBUG nextflow.cli.CmdRun - Applied DSL=2 from script declararion -Mar-08 14:13:17.534 [main] INFO nextflow.cli.CmdRun - Launching `/Users/annick.renevey/Projects/rnafusion/tests/../main.nf` [cheesy_hawking] DSL2 - revision: 12cef51b71 -Mar-08 14:13:17.536 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins declared=[nf-validation@1.1.3] -Mar-08 14:13:17.541 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins default=[] -Mar-08 14:13:17.541 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins resolved requirement=[nf-validation@1.1.3] -Mar-08 14:13:17.542 [main] DEBUG nextflow.plugin.PluginUpdater - Installing plugin nf-validation version: 1.1.3 -Mar-08 14:13:17.559 [main] INFO org.pf4j.AbstractPluginManager - Plugin 'nf-validation@1.1.3' resolved -Mar-08 14:13:17.560 [main] INFO org.pf4j.AbstractPluginManager - Start plugin 'nf-validation@1.1.3' -Mar-08 14:13:17.592 [main] DEBUG nextflow.plugin.BasePlugin - Plugin started nf-validation@1.1.3 -Mar-08 14:13:17.616 [main] DEBUG nextflow.secret.LocalSecretsProvider - Secrets store: /Users/annick.renevey/.nextflow/secrets/store.json -Mar-08 14:13:17.628 [main] DEBUG nextflow.secret.SecretsLoader - Discovered secrets providers: [nextflow.secret.LocalSecretsProvider@57e03347] - activable => nextflow.secret.LocalSecretsProvider@57e03347 -Mar-08 14:13:17.713 [main] DEBUG nextflow.Session - Session UUID: 1bbe00d2-1928-4556-b97c-ecef4af4222e -Mar-08 14:13:17.713 [main] DEBUG nextflow.Session - Run name: cheesy_hawking -Mar-08 14:13:17.714 [main] DEBUG nextflow.Session - Executor pool size: 8 -Mar-08 14:13:17.727 [main] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'FileTransfer' minSize=10; maxSize=24; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false -Mar-08 14:13:17.787 [main] DEBUG nextflow.cli.CmdRun - - Version: 23.04.1 build 5866 - Created: 15-04-2023 06:51 UTC (08:51 CEST) - System: Mac OS X 14.2.1 - Runtime: Groovy 3.0.16 on OpenJDK 64-Bit Server VM 17.0.3+7-LTS - Encoding: UTF-8 (UTF-8) - Process: 89543@annickrmbp [130.229.140.244] - CPUs: 8 - Mem: 16 GB (22.8 MB) - Swap: 6 GB (1.4 GB) -Mar-08 14:13:17.818 [main] DEBUG nextflow.Session - Work-dir: /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/work [Mac OS X] -Mar-08 14:13:17.840 [main] DEBUG nextflow.executor.ExecutorFactory - Extension executors providers=[] -Mar-08 14:13:17.857 [main] DEBUG nextflow.Session - Observer factory: DefaultObserverFactory -Mar-08 14:13:17.959 [main] DEBUG nextflow.cache.CacheFactory - Using Nextflow cache factory: nextflow.cache.DefaultCacheFactory -Mar-08 14:13:17.990 [main] DEBUG nextflow.util.CustomThreadPool - Creating default thread pool > poolSize: 9; maxThreads: 1000 -Mar-08 14:13:18.140 [main] DEBUG nextflow.Session - Session start -Mar-08 14:13:18.175 [main] DEBUG nextflow.trace.TraceFileObserver - Workflow started -- trace file: /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/trace.csv -Mar-08 14:13:18.579 [main] DEBUG nextflow.script.ScriptRunner - > Launching execution -Mar-08 14:13:19.365 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [paramsHelp:paramsHelp]; plugin Id: nf-validation -Mar-08 14:13:19.385 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [paramsSummaryLog:paramsSummaryLog]; plugin Id: nf-validation -Mar-08 14:13:19.385 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [validateParameters:validateParameters]; plugin Id: nf-validation -Mar-08 14:13:19.411 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [paramsSummaryMap:paramsSummaryMap]; plugin Id: nf-validation -Mar-08 14:13:19.413 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [fromSamplesheet:fromSamplesheet]; plugin Id: nf-validation -Mar-08 14:13:22.583 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [paramsHelp:paramsHelp]; plugin Id: nf-validation -Mar-08 14:13:22.586 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [paramsSummaryLog:paramsSummaryLog]; plugin Id: nf-validation -Mar-08 14:13:22.586 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [validateParameters:validateParameters]; plugin Id: nf-validation -Mar-08 14:13:22.588 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [paramsSummaryMap:paramsSummaryMap]; plugin Id: nf-validation -Mar-08 14:13:22.588 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [fromSamplesheet:fromSamplesheet]; plugin Id: nf-validation -Mar-08 14:13:22.738 [main] DEBUG nextflow.script.IncludeDef - Loading included plugin extensions with names: [paramsSummaryLog:paramsSummaryLog, paramsSummaryMap:paramsSummaryMap]; plugin Id: nf-validation -Mar-08 14:13:27.381 [main] DEBUG nextflow.Nextflow - Using schema file: nextflow_schema.json -Mar-08 14:13:27.389 [main] WARN nextflow.script.ScriptBinding - Access to undefined parameter `monochromeLogs` -- Initialise it to a default value eg. `params.monochromeLogs = some_value` -Mar-08 14:13:27.418 [main] INFO nextflow.Nextflow - - ------------------------------------------------------- - ,--./,-. - ___ __ __ __ ___ /,-._.--~' - |\ | |__ __ / ` / \ |__) |__ } { - | \| | \__, \__/ | \ |___ \`-._,-`-, - `._,._,' - nf-core/rnafusion v3.0.2dev ------------------------------------------------------- -Core Nextflow options - runName : cheesy_hawking - containerEngine : docker - launchDir : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997 - workDir : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/work - projectDir : /Users/annick.renevey/Projects/rnafusion - userName : annick.renevey - profile : debug,test,docker - configFiles :  - -Input/output options - input : https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv - outdir : results - build_references : true - genomes_base : results/references - starfusion_build : true - arriba_ref : results/references/arriba - arriba_ref_blacklist : results/references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz - arriba_ref_cytobands : results/references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv - arriba_ref_known_fusions : results/references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz - arriba_ref_protein_domains : results/references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3 - ensembl_ref : results/references/ensembl - fusioncatcher_limitSjdbInsertNsj : 2000000 - fusioncatcher_ref : results/references/fusioncatcher/human_v102 - fusioninspector_limitSjdbInsertNsj: 1000000 - fusionreport_ref : results/references/fusion_report_db - hgnc_ref : results/references/hgnc/hgnc_complete_set.txt - hgnc_date : results/references/hgnc/HGNC-DB-timestamp.txt - starfusion_ref : results/references/starfusion/ctat_genome_lib_build_dir - starindex_ref : results/references/star - tools_cutoff : 1 - -Read trimming options - adapter_fasta : [] - -Alignment compression options - cram : [] - -Reference genome options - genome : GRCh38 - fasta : results/references/ensembl/Homo_sapiens.GRCh38.102.all.fa - fai : results/references/ensembl/Homo_sapiens.GRCh38.102.all.fa.fai - gtf : results/references/ensembl/Homo_sapiens.GRCh38.102.gtf - chrgtf : results/references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf - transcript : results/references/ensembl/Homo_sapiens.GRCh38.102.cdna.all.fa.gz - refflat : results/references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf.refflat - rrna_intervals : results/references/ensembl/Homo_sapiens.GRCh38.102.interval_list - -Institutional config options - config_profile_name : Test profile - config_profile_description : Minimal test dataset to check pipeline function - -Max job request options - max_cpus : 2 - max_memory : 6.GB - max_time : 6.h - -!! Only displaying parameters that differ from the pipeline defaults !! ------------------------------------------------------- -If you use nf-core/rnafusion for your analysis please cite: - -* The pipeline - - -* The nf-core framework - https://doi.org/10.1038/s41587-020-0439-x - -* Software dependencies - https://github.com/nf-core/rnafusion/blob/master/CITATIONS.md ------------------------------------------------------- -Mar-08 14:13:27.425 [main] DEBUG nextflow.validation.SchemaValidator - Starting parameters validation -Mar-08 14:13:27.617 [main] WARN nextflow.validation.SchemaValidator - The following invalid input values have been detected: - -* --genome_base: references -* --modules_testdata_base_path: s3://ngi-igenomes/testdata/nf-core/modules/ -* --pipelines_testdata_base_path: s3://ngi-igenomes/testdata/nf-core/pipelines/rnaseq/3.15/ - - -Mar-08 14:13:27.912 [main] DEBUG nextflow.validation.SchemaValidator - Starting validation: 'input': '/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv' with '/Users/annick.renevey/Projects/rnafusion/assets/schema_input.json' -Mar-08 14:13:29.935 [main] DEBUG nextflow.validation.SchemaValidator - Validation passed: 'input': '/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv' with '/Users/annick.renevey/Projects/rnafusion/assets/schema_input.json' -Mar-08 14:13:29.935 [main] DEBUG nextflow.validation.SchemaValidator - Finishing parameters validation -Mar-08 14:13:30.046 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD -Mar-08 14:13:30.049 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:ENSEMBL_DOWNLOAD` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD -Mar-08 14:13:30.050 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD -Mar-08 14:13:30.053 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD -Mar-08 14:13:30.061 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null -Mar-08 14:13:30.061 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' -Mar-08 14:13:30.083 [main] DEBUG nextflow.executor.Executor - [warm up] executor > local -Mar-08 14:13:30.090 [main] DEBUG n.processor.LocalPollingMonitor - Creating local task monitor for executor 'local' > cpus=8; memory=16 GB; capacity=8; pollInterval=100ms; dumpInterval=5m -Mar-08 14:13:30.203 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD -Mar-08 14:13:30.204 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:HGNC_DOWNLOAD` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD -Mar-08 14:13:30.206 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD -Mar-08 14:13:30.207 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD -Mar-08 14:13:30.213 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null -Mar-08 14:13:30.214 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' -Mar-08 14:13:30.236 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_single` matches labels `process_single` for process with name NFCORE_RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX -Mar-08 14:13:30.237 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:SAMTOOLS_FAIDX` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX -Mar-08 14:13:30.238 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX -Mar-08 14:13:30.238 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX -Mar-08 14:13:30.240 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null -Mar-08 14:13:30.240 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' -Mar-08 14:13:30.248 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_CREATESEQUENCEDICTIONARY -Mar-08 14:13:30.248 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_CREATESEQUENCEDICTIONARY -Mar-08 14:13:30.249 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_CREATESEQUENCEDICTIONARY -Mar-08 14:13:30.250 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null -Mar-08 14:13:30.250 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' -Mar-08 14:13:30.263 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_single` matches labels `process_single` for process with name NFCORE_RNAFUSION:BUILD_REFERENCES:RRNA_TRANSCRIPTS -Mar-08 14:13:30.264 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:RRNA_TRANSCRIPTS -Mar-08 14:13:30.264 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:RRNA_TRANSCRIPTS -Mar-08 14:13:30.265 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null -Mar-08 14:13:30.265 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' -Mar-08 14:13:30.273 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_single` matches labels `process_single` for process with name NFCORE_RNAFUSION:BUILD_REFERENCES:CONVERT2BED -Mar-08 14:13:30.274 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:CONVERT2BED -Mar-08 14:13:30.274 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:CONVERT2BED -Mar-08 14:13:30.274 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null -Mar-08 14:13:30.275 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' -Mar-08 14:13:30.290 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST -Mar-08 14:13:30.292 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:GATK4_BEDTOINTERVALLIST` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST -Mar-08 14:13:30.292 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST -Mar-08 14:13:30.292 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST -Mar-08 14:13:30.294 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null -Mar-08 14:13:30.294 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' -Mar-08 14:13:30.302 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_low` matches labels `process_low` for process with name NFCORE_RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT -Mar-08 14:13:30.303 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:GTF_TO_REFFLAT` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT -Mar-08 14:13:30.304 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT -Mar-08 14:13:30.304 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process NFCORE_RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT -Mar-08 14:13:30.306 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null -Mar-08 14:13:30.306 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' -Mar-08 14:13:30.322 [main] DEBUG nextflow.Session - Workflow process names [dsl2]: SAMTOOLS_VIEW_FOR_STARFUSION, SAMTOOLS_SORT_FOR_ARRIBA, GATK4_BEDTOINTERVALLIST, STAR_FOR_ARRIBA, STARFUSION_DOWNLOAD, FUSIONCATCHER, FUSIONCATCHER_DOWNLOAD, STAR_FOR_STARFUSION, GATK4_CREATESEQUENCEDICTIONARY, FASTP, FASTQC_FOR_FASTP, ENSEMBL_DOWNLOAD, GATK4_MARKDUPLICATES, CAT_FASTQ, GTF_TO_REFFLAT, STARFUSION, NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST, NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_CREATESEQUENCEDICTIONARY, NFCORE_RNAFUSION:BUILD_REFERENCES:CONVERT2BED, VCF_COLLECT, SAMTOOLS_INDEX_FOR_ARRIBA, ARRIBA_VISUALISATION, CONVERT2BED, FUSIONREPORT_DOWNLOAD, STAR_ALIGN, SAMTOOLS_INDEX_FOR_STARFUSION, FUSIONINSPECTOR, SAMTOOLS_INDEX, FASTQC, ARRIBA_DOWNLOAD, NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD, MULTIQC, NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD, STRINGTIE_MERGE, SAMTOOLS_SORT, SAMTOOLS_INDEX_FOR_STARFUSION_CRAM, SAMTOOLS_VIEW_FOR_ARRIBA, NFCORE_RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT, NFCORE_RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX, STARFUSION_BUILD, CAT_CAT, PICARD_COLLECTRNASEQMETRICS, HGNC_DOWNLOAD, SAMTOOLS_VIEW, SAMTOOLS_FAIDX, FUSIONREPORT, RRNA_TRANSCRIPTS, AGAT_CONVERTSPGFF2TSV, STRINGTIE_STRINGTIE, PICARD_COLLECTINSERTSIZEMETRICS, ARRIBA, NFCORE_RNAFUSION:BUILD_REFERENCES:RRNA_TRANSCRIPTS, STAR_GENOMEGENERATE -Mar-08 14:13:30.337 [main] WARN nextflow.Session - There's no process matching config selector: SAMPLESHEET_CHECK -Mar-08 14:13:30.337 [main] DEBUG nextflow.Session - Igniting dataflow network (25) -Mar-08 14:13:30.369 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD -Mar-08 14:13:30.370 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD -Mar-08 14:13:30.371 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX -Mar-08 14:13:30.371 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_CREATESEQUENCEDICTIONARY -Mar-08 14:13:30.372 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNAFUSION:BUILD_REFERENCES:RRNA_TRANSCRIPTS -Mar-08 14:13:30.372 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNAFUSION:BUILD_REFERENCES:CONVERT2BED -Mar-08 14:13:30.373 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST -Mar-08 14:13:30.373 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > NFCORE_RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT -Mar-08 14:13:30.373 [main] DEBUG nextflow.script.ScriptRunner - > Awaiting termination -Mar-08 14:13:30.373 [main] DEBUG nextflow.Session - Session await -Mar-08 14:13:30.553 [Actor Thread 17] INFO nextflow.processor.TaskProcessor - [NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD] cache hash: 7f18070817f5b2ac14c8e745abfb6933; mode: STANDARD; entries: - 338337db5eff3bff24c213e2d8964e63 [java.util.UUID] 1bbe00d2-1928-4556-b97c-ecef4af4222e - 5dd471f5f4c9a54dea64867d4265ee28 [java.lang.String] NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD - 9ff7290eb13332bf5c4063dc712689d6 [java.lang.String] """ - wget https://ftp.ebi.ac.uk/pub/databases/genenames/hgnc/tsv/hgnc_complete_set.txt - date +%Y-%m-%d/%H:%M > HGNC-DB-timestamp.txt - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml) - END_VERSIONS - """ - - 00c7c646a986ea5502fb404ea15e7443 [java.lang.String] quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5 - 4f9d4b0d22865056c37fb6d9c2a04a67 [java.lang.String] $ - 16fe7483905cce7a85670e43e4678877 [java.lang.Boolean] true - 77476d335f7c3b5b95495d42532f58d0 [java.util.HashMap$EntrySet] [task.process=NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD] - -Mar-08 14:13:30.613 [Actor Thread 15] INFO nextflow.processor.TaskProcessor - [NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD] cache hash: dd31277bda420c7e0145fc89ea8613e5; mode: STANDARD; entries: - 338337db5eff3bff24c213e2d8964e63 [java.util.UUID] 1bbe00d2-1928-4556-b97c-ecef4af4222e - f65e11932b552ca585de284c5e5d0162 [java.lang.String] NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD - 0955cd72953c2b84dffe718d8e97dc15 [java.lang.String] """ - wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{1..22}.fa.gz - wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{MT,X,Y}.fa.gz - - wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz - wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz - wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz - - gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}.${ensembl_version}.all.fa - gunzip Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz - gunzip Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz - - - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml) - END_VERSIONS - """ - - 00c7c646a986ea5502fb404ea15e7443 [java.lang.String] quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5 - fee0e11be1152749b19f3d19542d3901 [java.lang.String] ensembl_version - f56f0c2893db292d86b93804fe80b6ac [java.lang.Integer] 102 - d9c152f5939e0f93a25dbd62b7c4d0a5 [java.lang.String] genome - 6b15613e5b490261f4ffe786a92c5825 [java.lang.String] GRCh38 - 22a9e5e0e97f0475e310f04c56b1393a [java.lang.String] meta - cb0f105a57d4145f7acb92ca9cf2ea12 [java.util.LinkedHashMap] [id:Homo_sapiens.GRCh38.102] - 4f9d4b0d22865056c37fb6d9c2a04a67 [java.lang.String] $ - 16fe7483905cce7a85670e43e4678877 [java.lang.Boolean] true - c877f2c88b6ad8c3dbe3236a80f57755 [java.util.HashMap$EntrySet] [task.process=NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD, params.genome=GRCh38] - -Mar-08 14:13:30.754 [Task submitter] DEBUG n.executor.local.LocalTaskHandler - Launch cmd line: /bin/bash -ue .command.run -Mar-08 14:13:30.756 [Task submitter] INFO nextflow.Session - [81/2c2c1f] Submitted process > NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD (hgnc) -Mar-08 14:13:30.864 [Task submitter] DEBUG n.executor.local.LocalTaskHandler - Launch cmd line: /bin/bash -ue .command.run -Mar-08 14:13:30.865 [Task submitter] INFO nextflow.Session - [52/33e328] Submitted process > NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD (ensembl) -Mar-08 14:14:06.817 [Task monitor] DEBUG n.processor.TaskPollingMonitor - Task completed > TaskHandler[id: 1; name: NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD (hgnc); status: COMPLETED; exit: 0; error: -; workDir: /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/work/81/2c2c1f77cfe71f861687ce1e2e35ba] -Mar-08 14:14:06.983 [Task monitor] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'PublishDir' minSize=10; maxSize=24; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false -Mar-08 14:14:10.653 [SIGINT handler] DEBUG nextflow.Session - Session aborted -- Cause: SIGINT -Mar-08 14:14:10.717 [SIGINT handler] DEBUG nextflow.Session - The following nodes are still active: -[process] NFCORE_RNAFUSION:BUILD_REFERENCES:SAMTOOLS_FAIDX - status=ACTIVE - port 0: (value) OPEN ; channel: - - port 1: (value) bound ; channel: - - port 2: (cntrl) - ; channel: $ - -[process] NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_CREATESEQUENCEDICTIONARY - status=ACTIVE - port 0: (value) OPEN ; channel: - - port 1: (cntrl) - ; channel: $ - -[process] NFCORE_RNAFUSION:BUILD_REFERENCES:RRNA_TRANSCRIPTS - status=ACTIVE - port 0: (value) OPEN ; channel: - - port 1: (cntrl) - ; channel: $ - -[process] NFCORE_RNAFUSION:BUILD_REFERENCES:CONVERT2BED - status=ACTIVE - port 0: (value) OPEN ; channel: - - port 1: (cntrl) - ; channel: $ - -[process] NFCORE_RNAFUSION:BUILD_REFERENCES:GATK4_BEDTOINTERVALLIST - status=ACTIVE - port 0: (value) OPEN ; channel: - - port 1: (value) OPEN ; channel: - - port 2: (cntrl) - ; channel: $ - -[process] NFCORE_RNAFUSION:BUILD_REFERENCES:GTF_TO_REFFLAT - status=ACTIVE - port 0: (value) OPEN ; channel: - - port 1: (cntrl) - ; channel: $ - -Mar-08 14:14:10.812 [main] DEBUG nextflow.Session - Session await > all processes finished -Mar-08 14:14:10.813 [main] DEBUG nextflow.Session - Session await > all barriers passed -Mar-08 14:14:10.829 [main] INFO nextflow.Nextflow - -[nf-core/rnafusion] Pipeline completed with errors- -Mar-08 14:14:10.840 [main] WARN n.processor.TaskPollingMonitor - Killing running tasks (1) -Mar-08 14:14:10.874 [main] DEBUG nextflow.trace.WorkflowStatsObserver - Workflow completed > WorkflowStats[succeededCount=1; failedCount=0; ignoredCount=0; cachedCount=0; pendingCount=0; submittedCount=0; runningCount=1; retriesCount=0; abortedCount=0; succeedDuration=52s; failedDuration=0ms; cachedDuration=0ms;loadCpus=0; loadMemory=0; peakRunning=2; peakCpus=4; peakMemory=6 GB; ] -Mar-08 14:14:10.875 [main] DEBUG nextflow.trace.TraceFileObserver - Workflow completed -- saving trace file -Mar-08 14:14:10.897 [main] DEBUG nextflow.cache.CacheDB - Closing CacheDB done -Mar-08 14:14:10.897 [main] INFO org.pf4j.AbstractPluginManager - Stop plugin 'nf-validation@1.1.3' -Mar-08 14:14:10.898 [main] DEBUG nextflow.plugin.BasePlugin - Plugin stopped nf-validation -Mar-08 14:14:11.002 [main] DEBUG nextflow.script.ScriptRunner - > Execution complete -- Goodbye diff --git a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/params.json b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/params.json deleted file mode 100644 index 691664e7..00000000 --- a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/params.json +++ /dev/null @@ -1,11 +0,0 @@ -{ - "input": "https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv", - "cosmic_username": null, - "build_references": true, - "genome_base": "references", - "cosmic_passwd": null, - "max_time": "6.h", - "max_cpus": 2, - "max_memory": "6.GB", - "outdir": "results" -} diff --git a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/std.err b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/std.err deleted file mode 100644 index e69de29b..00000000 diff --git a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/std.out b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/std.out deleted file mode 100644 index 7b566f86..00000000 --- a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/std.out +++ /dev/null @@ -1,145 +0,0 @@ -N E X T F L O W ~ version 23.04.1 -Launching `/Users/annick.renevey/Projects/rnafusion/tests/../main.nf` [cheesy_hawking] DSL2 - revision: 12cef51b71 -WARN: Access to undefined parameter `monochromeLogs` -- Initialise it to a default value eg. `params.monochromeLogs = some_value` - - ------------------------------------------------------- - ,--./,-. - ___ __ __ __ ___ /,-._.--~' - |\ | |__ __ / ` / \ |__) |__ } { - | \| | \__, \__/ | \ |___ \`-._,-`-, - `._,._,' - nf-core/rnafusion v3.0.2dev ------------------------------------------------------- -Core Nextflow options - runName : cheesy_hawking - containerEngine : docker - launchDir : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997 - workDir : /Users/annick.renevey/Projects/rnafusion/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/work - projectDir : /Users/annick.renevey/Projects/rnafusion - userName : annick.renevey - profile : debug,test,docker - configFiles :  - -Input/output options - input : https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv - outdir : results - build_references : true - genomes_base : results/references - starfusion_build : true - arriba_ref : results/references/arriba - arriba_ref_blacklist : results/references/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz - arriba_ref_cytobands : results/references/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv - arriba_ref_known_fusions : results/references/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz - arriba_ref_protein_domains : results/references/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3 - ensembl_ref : results/references/ensembl - fusioncatcher_limitSjdbInsertNsj : 2000000 - fusioncatcher_ref : results/references/fusioncatcher/human_v102 - fusioninspector_limitSjdbInsertNsj: 1000000 - fusionreport_ref : results/references/fusion_report_db - hgnc_ref : results/references/hgnc/hgnc_complete_set.txt - hgnc_date : results/references/hgnc/HGNC-DB-timestamp.txt - starfusion_ref : results/references/starfusion/ctat_genome_lib_build_dir - starindex_ref : results/references/star - tools_cutoff : 1 - -Read trimming options - adapter_fasta : [] - -Alignment compression options - cram : [] - -Reference genome options - genome : GRCh38 - fasta : results/references/ensembl/Homo_sapiens.GRCh38.102.all.fa - fai : results/references/ensembl/Homo_sapiens.GRCh38.102.all.fa.fai - gtf : results/references/ensembl/Homo_sapiens.GRCh38.102.gtf - chrgtf : results/references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf - transcript : results/references/ensembl/Homo_sapiens.GRCh38.102.cdna.all.fa.gz - refflat : results/references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf.refflat - rrna_intervals : results/references/ensembl/Homo_sapiens.GRCh38.102.interval_list - -Institutional config options - config_profile_name : Test profile - config_profile_description : Minimal test dataset to check pipeline function - -Max job request options - max_cpus : 2 - max_memory : 6.GB - max_time : 6.h - -!! Only displaying parameters that differ from the pipeline defaults !! ------------------------------------------------------- -If you use nf-core/rnafusion for your analysis please cite: - -* The pipeline - - -* The nf-core framework - https://doi.org/10.1038/s41587-020-0439-x - -* Software dependencies - https://github.com/nf-core/rnafusion/blob/master/CITATIONS.md ------------------------------------------------------- -WARN: The following invalid input values have been detected: - -* --genome_base: references -* --modules_testdata_base_path: s3://ngi-igenomes/testdata/nf-core/modules/ -* --pipelines_testdata_base_path: s3://ngi-igenomes/testdata/nf-core/pipelines/rnaseq/3.15/ - - -WARN: There's no process matching config selector: SAMPLESHEET_CHECK -[NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD] cache hash: 7f18070817f5b2ac14c8e745abfb6933; mode: STANDARD; entries: - 338337db5eff3bff24c213e2d8964e63 [java.util.UUID] 1bbe00d2-1928-4556-b97c-ecef4af4222e - 5dd471f5f4c9a54dea64867d4265ee28 [java.lang.String] NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD - 9ff7290eb13332bf5c4063dc712689d6 [java.lang.String] """ - wget https://ftp.ebi.ac.uk/pub/databases/genenames/hgnc/tsv/hgnc_complete_set.txt - date +%Y-%m-%d/%H:%M > HGNC-DB-timestamp.txt - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml) - END_VERSIONS - """ - - 00c7c646a986ea5502fb404ea15e7443 [java.lang.String] quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5 - 4f9d4b0d22865056c37fb6d9c2a04a67 [java.lang.String] $ - 16fe7483905cce7a85670e43e4678877 [java.lang.Boolean] true - 77476d335f7c3b5b95495d42532f58d0 [java.util.HashMap$EntrySet] [task.process=NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD] - -[NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD] cache hash: dd31277bda420c7e0145fc89ea8613e5; mode: STANDARD; entries: - 338337db5eff3bff24c213e2d8964e63 [java.util.UUID] 1bbe00d2-1928-4556-b97c-ecef4af4222e - f65e11932b552ca585de284c5e5d0162 [java.lang.String] NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD - 0955cd72953c2b84dffe718d8e97dc15 [java.lang.String] """ - wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{1..22}.fa.gz - wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/dna/Homo_sapiens.${params.genome}.dna.chromosome.{MT,X,Y}.fa.gz - - wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz - wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/gtf/homo_sapiens/Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz - wget ftp://ftp.ensembl.org/pub/release-${ensembl_version}/fasta/homo_sapiens/cdna/Homo_sapiens.${params.genome}.cdna.all.fa.gz -O Homo_sapiens.${params.genome}.${ensembl_version}.cdna.all.fa.gz - - gunzip -c Homo_sapiens.${params.genome}.dna.chromosome.* > Homo_sapiens.${params.genome}.${ensembl_version}.all.fa - gunzip Homo_sapiens.${params.genome}.${ensembl_version}.gtf.gz - gunzip Homo_sapiens.${params.genome}.${ensembl_version}.chr.gtf.gz - - - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - wget: \$(echo wget -V 2>&1 | grep "GNU Wget" | cut -d" " -f3 > versions.yml) - END_VERSIONS - """ - - 00c7c646a986ea5502fb404ea15e7443 [java.lang.String] quay.io/biocontainers/gnu-wget:1.18--h5bf99c6_5 - fee0e11be1152749b19f3d19542d3901 [java.lang.String] ensembl_version - f56f0c2893db292d86b93804fe80b6ac [java.lang.Integer] 102 - d9c152f5939e0f93a25dbd62b7c4d0a5 [java.lang.String] genome - 6b15613e5b490261f4ffe786a92c5825 [java.lang.String] GRCh38 - 22a9e5e0e97f0475e310f04c56b1393a [java.lang.String] meta - cb0f105a57d4145f7acb92ca9cf2ea12 [java.util.LinkedHashMap] [id:Homo_sapiens.GRCh38.102] - 4f9d4b0d22865056c37fb6d9c2a04a67 [java.lang.String] $ - 16fe7483905cce7a85670e43e4678877 [java.lang.Boolean] true - c877f2c88b6ad8c3dbe3236a80f57755 [java.util.HashMap$EntrySet] [task.process=NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD, params.genome=GRCh38] - -[81/2c2c1f] Submitted process > NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD (hgnc) -[52/33e328] Submitted process > NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD (ensembl) diff --git a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/trace.csv b/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/trace.csv deleted file mode 100644 index 57aed492..00000000 --- a/.nf-test/tests/818076bdee20d7e7df39ccd4d6617997/meta/trace.csv +++ /dev/null @@ -1,3 +0,0 @@ -task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar -1 81/2c2c1f 89776 NFCORE_RNAFUSION:BUILD_REFERENCES:HGNC_DOWNLOAD (hgnc) COMPLETED 0 2024-03-08 14:13:30.756 36s 26s 3.0% 24.8 MB 2.3 GB 16.2 MB 15.8 MB -2 52/33e328 89787 NFCORE_RNAFUSION:BUILD_REFERENCES:ENSEMBL_DOWNLOAD (ensembl) ABORTED - 2024-03-08 14:13:30.864 - - - - - - - From 9313a47e5c62830c66d580589098b87cad2d54e9 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 15 Mar 2024 15:33:33 +0200 Subject: [PATCH 47/64] fix ci --- main.nf | 13 ++++++++++++- workflows/rnafusion.nf | 7 ++++++- 2 files changed, 18 insertions(+), 2 deletions(-) diff --git a/main.nf b/main.nf index 8ba6f049..ac96ab66 100644 --- a/main.nf +++ b/main.nf @@ -24,6 +24,16 @@ include { BUILD_REFERENCES } from './workflows/build_references' include { RNAFUSION } from './workflows/rnafusion' +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + GENOME PARAMETER VALUES +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + + + + + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ NAMED WORKFLOWS FOR PIPELINE @@ -43,7 +53,8 @@ workflow NFCORE_RNAFUSION { if (params.build_references) { BUILD_REFERENCES () } else { - RNAFUSION() + ch_samplesheet = Channel.value(file(params.input, checkIfExists: true)) + RNAFUSION(ch_samplesheet) } } diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf index a1b8f431..511b121b 100644 --- a/workflows/rnafusion.nf +++ b/workflows/rnafusion.nf @@ -68,6 +68,11 @@ include { CAT_FASTQ } from '../modules/nf-core/cat/fastq/main' workflow RNAFUSION { + take: + ch_samplesheet + + main: + ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() @@ -77,7 +82,7 @@ workflow RNAFUSION { Channel .fromSamplesheet("input") .map { - meta, fastq_1, fastq_2 -> + meta, fastq_1, fastq_2, strandedness -> if (!fastq_2) { return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] } else { From 1ea6a3aff55d067a17097f3a2bdd7a6847c9a6e1 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 15 Mar 2024 16:29:10 +0200 Subject: [PATCH 48/64] add // --- .../utils_nfcore_rnafusion_pipeline/main.nf | 23 +++++++++++++++++++ 1 file changed, 23 insertions(+) diff --git a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf index e7d4e72d..3a02b058 100644 --- a/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_rnafusion_pipeline/main.nf @@ -233,3 +233,26 @@ def methodsDescriptionText(mqc_methods_yaml) { return description_html.toString() } + +// +// Function to generate an error if contigs in genome fasta file > 512 Mbp +// +def checkMaxContigSize(fai_file) { + def max_size = 512000000 + fai_file.eachLine { line -> + def lspl = line.split('\t') + def chrom = lspl[0] + def size = lspl[1] + if (size.toInteger() > max_size) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Contig longer than ${max_size}bp found in reference genome!\n\n" + + " ${chrom}: ${size}\n\n" + + " Provide the '--bam_csi_index' parameter to use a CSI instead of BAI index.\n\n" + + " Please see:\n" + + " https://github.com/nf-core/rnaseq/issues/744\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + error(error_string) + } + } +} + From 6e2408689ab6d5f1c9a605d284ae0c20e6e87cf7 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Fri, 15 Mar 2024 16:29:23 +0200 Subject: [PATCH 49/64] checkMaxContigSize function --- 512 | 0 1 file changed, 0 insertions(+), 0 deletions(-) create mode 100644 512 diff --git a/512 b/512 new file mode 100644 index 00000000..e69de29b From a66ec8479e159e24413e9786793744d58705e313 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 18 Mar 2024 14:10:23 +0100 Subject: [PATCH 50/64] bugfix for vcf --- bin/vcf_collect.py | 69 ++++++++++++++++++++++++++++++++-------------- 1 file changed, 49 insertions(+), 20 deletions(-) diff --git a/bin/vcf_collect.py b/bin/vcf_collect.py index 7e988c1e..5b73a92e 100755 --- a/bin/vcf_collect.py +++ b/bin/vcf_collect.py @@ -65,7 +65,9 @@ def vcf_collect( gtf_df = build_gtf_dataframe(gtf) all_df = df.merge(gtf_df, how="left", left_on="CDS_LEFT_ID", right_on="Transcript_id") - all_df[["PosA", "orig_start", "orig_end"]] = all_df[["PosA", "orig_start", "orig_end"]].fillna(0).astype(int) + all_df[["PosA", "orig_start", "orig_end"]] = ( + all_df[["PosA", "orig_start", "orig_end"]].fillna(0).astype(int) + ) all_df = all_df[ ((all_df["PosA"] >= all_df["orig_start"]) & (all_df["PosA"] <= all_df["orig_end"])) @@ -75,7 +77,9 @@ def vcf_collect( all_df.replace("", np.nan, inplace=True) all_df = all_df.drop_duplicates() - all_df[["exon_number", "transcript_version"]] = all_df[["exon_number", "transcript_version"]].replace(0, np.nan) + all_df[["exon_number", "transcript_version"]] = all_df[ + ["exon_number", "transcript_version"] + ].replace(0, np.nan) # Fill non-empty values within each group for 'exon_number' and 'transcript_version' all_df["exon_number"] = all_df.groupby("PosA")["exon_number"].transform( lambda x: x.fillna(method="ffill").fillna(method="bfill") @@ -116,8 +120,12 @@ def vcf_collect( ].drop_duplicates() all_df["CDS_RIGHT_ID"] = all_df["CDS_RIGHT_ID"].astype("str") all_df = all_df.merge(gtf_df, how="left", left_on="CDS_RIGHT_ID", right_on="Transcript_id") - all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].fillna(0) - all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].astype(int) + all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].fillna( + 0 + ) + all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].astype( + int + ) all_df = all_df[ ((all_df["PosB"] >= all_df["orig_start"]) & (all_df["PosB"] <= all_df["orig_end"])) | ((all_df["orig_start"] == 0) & (all_df["orig_end"] == 0)) @@ -126,7 +134,9 @@ def vcf_collect( all_df[["PosA", "PosB"]] = all_df[["PosA", "PosB"]].replace(0, np.nan) all_df = all_df.replace("", np.nan) - all_df[["exon_number", "transcript_version"]] = all_df[["exon_number", "transcript_version"]].replace(0, np.nan) + all_df[["exon_number", "transcript_version"]] = all_df[ + ["exon_number", "transcript_version"] + ].replace(0, np.nan) # Fill non-empty values within each group for 'exon_number' and 'transcript_version' all_df["exon_number"] = all_df.groupby("PosB")["exon_number"].transform( lambda x: x.fillna(method="ffill").fillna(method="bfill") @@ -212,7 +222,9 @@ def parse_args(argv=None): type=Path, help="HGNC database.", ) - parser.add_argument("--sample", metavar="SAMPLE", type=Path, help="Sample name.", default="Sample") + parser.add_argument( + "--sample", metavar="SAMPLE", type=Path, help="Sample name.", default="Sample" + ) parser.add_argument( "--out", metavar="OUT", @@ -280,7 +292,11 @@ def build_fusioninspector_dataframe(file: str) -> pd.DataFrame: df["annots"] = ( df["annots"] .apply(convert_to_list) - .apply(lambda x: ",".join(map(str, x)) if isinstance(x, list) else str(x) if pd.notna(x) else "") + .apply( + lambda x: ( + ",".join(map(str, x)) if isinstance(x, list) else str(x) if pd.notna(x) else "" + ) + ) ) else: for i in [ @@ -334,9 +350,10 @@ def read_build_fusionreport(fusionreport_file: str) -> pd.DataFrame: Make all column headers uppercase. """ with open(fusionreport_file) as f: - from_html = [line.split('rows": [')[1] for line in f if 'name="fusion_list' in line] - expression = ast.literal_eval(from_html[0].split('], "tool')[0]) - fusion_report = pd.DataFrame.from_dict({k: [v] for k, v in expression.items()}) + from_html = [line.split('rows": ')[1] for line in f if 'name="fusion_list' in line] + tmp=str(from_html)[2:] + tmp2 = (tmp.split(', "tools": ')[0]) + fusion_report = pd.DataFrame(ast.literal_eval(tmp2)) if not "arriba" in fusion_report.columns: fusion_report["arriba"] = "" if not "fusioncatcher" in fusion_report.columns: @@ -356,12 +373,12 @@ def read_build_fusionreport(fusionreport_file: str) -> pd.DataFrame: concatenate_columns, axis=1 ) fusion_report.columns = fusion_report.columns.str.upper() - fusion_report["FOUND_DB"] = fusion_report["FOUND_DB"].apply(lambda x: ",".join(x)) + fusion_report["FOUND_DB"] = fusion_report["FOUND_DB"].apply(lambda x: ",".join(x) if len(x) > 0 else '') fusion_report[["GeneA", "GeneB"]] = fusion_report["FUSION"].str.split("--", expand=True) - return fusion_report[["FUSION", "GeneA", "GeneB", "TOOLS_HITS", "SCORE", "FOUND_DB", "FOUND_IN"]].set_index( - ["FUSION"] - ) + return fusion_report[ + ["FUSION", "GeneA", "GeneB", "TOOLS_HITS", "SCORE", "FOUND_DB", "FOUND_IN"] + ].set_index(["FUSION"]) def read_fusionreport_csv(file: str) -> pd.DataFrame: @@ -370,7 +387,9 @@ def read_fusionreport_csv(file: str) -> pd.DataFrame: for column in columns_to_iterate: if column not in df.columns: df[column] = "" - df[["starfusion", "arriba", "fusioncatcher"]] = df[["starfusion", "arriba", "fusioncatcher"]].astype("str") + df[["starfusion", "arriba", "fusioncatcher"]] = df[ + ["starfusion", "arriba", "fusioncatcher"] + ].astype("str") for index, row in df.iterrows(): for column in columns_to_iterate: cell_value = row[column] @@ -398,7 +417,9 @@ def read_fusionreport_csv(file: str) -> pd.DataFrame: df[["GeneA", "GeneB"]] = df["Fusion"].str.split("--", expand=True) df = df.set_index("Fusion") df.to_csv("tmp.csv") - return df[["GeneA", "GeneB", "ChromosomeA", "PosA", "StrandA", "ChromosomeB", "PosB", "StrandB"]] + return df[ + ["GeneA", "GeneB", "ChromosomeA", "PosA", "StrandA", "ChromosomeB", "PosB", "StrandB"] + ] def column_manipulation(df: pd.DataFrame) -> pd.DataFrame: @@ -425,7 +446,9 @@ def column_manipulation(df: pd.DataFrame) -> pd.DataFrame: df["Left_exon_number"] = df["Left_exon_number"].fillna(0).astype(int).astype(str) df["Right_exon_number"] = df["Right_exon_number"].fillna(0).astype(int).astype(str) df["Left_transcript_version"] = df["Left_transcript_version"].fillna(0).astype(int).astype(str) - df["Right_transcript_version"] = df["Right_transcript_version"].fillna(0).astype(int).astype(str) + df["Right_transcript_version"] = ( + df["Right_transcript_version"].fillna(0).astype(int).astype(str) + ) df["PosA"] = df["PosA"].fillna(0).astype(int).astype(str) df["PosB"] = df["PosB"].fillna(0).astype(int).astype(str) df["PROT_FUSION_TYPE"] = df["PROT_FUSION_TYPE"].replace(".", "nan") @@ -452,7 +475,9 @@ def column_manipulation(df: pd.DataFrame) -> pd.DataFrame: f"EXON_NUMBER_A={row['Left_exon_number']};EXON_NUMBER_B={row['Right_exon_number']};" f"ANNOTATIONS={row['annots']}" ) - df.loc[index, "Sample"] = f"./1:{row['JunctionReadCount']}:{row['SpanningFragCount']}:{row['FFPM']}" + df.loc[index, "Sample"] = ( + f"./1:{row['JunctionReadCount']}:{row['SpanningFragCount']}:{row['FFPM']}" + ) return df @@ -497,7 +522,9 @@ def build_gtf_dataframe(file: str) -> pd.DataFrame: """ df = pd.read_csv(file, sep="\t") df[["fusion_dump", "Transcript_id"]] = df["transcript_id"].str.split("^", expand=True) - df[["orig_chromosome", "orig_start", "orig_end", "orig_dir"]] = df["orig_coord_info"].str.split(",", expand=True) + df[["orig_chromosome", "orig_start", "orig_end", "orig_dir"]] = df["orig_coord_info"].str.split( + ",", expand=True + ) return df[["Transcript_id", "transcript_version", "exon_number", "orig_start", "orig_end"]] @@ -511,7 +538,9 @@ def main(argv=None): or not args.fusionreport_csv or not args.hgnc ): - logger.error(f"The given input file {args.fusioninspector} or {args.fusionreport} was not found!") + logger.error( + f"The given input file {args.fusioninspector} or {args.fusionreport} was not found!" + ) sys.exit(2) vcf_collect( args.fusioninspector, From 3ec309015f0ce12c0c5d678075b00bfe19a30db8 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 18 Mar 2024 20:09:46 +0100 Subject: [PATCH 51/64] black without line limit --- bin/vcf_collect.py | 135 +++++++++++++++++++++++++++++++-------------- 1 file changed, 95 insertions(+), 40 deletions(-) diff --git a/bin/vcf_collect.py b/bin/vcf_collect.py index 5b73a92e..1decbe90 100755 --- a/bin/vcf_collect.py +++ b/bin/vcf_collect.py @@ -47,9 +47,14 @@ def vcf_collect( df_not_symbol = merged_df[merged_df["Left_ensembl_gene_id"].notna()] df_not_symbol = hgnc_df.merge( - df_not_symbol, how="right", left_on="ensembl_gene_id", right_on="Left_ensembl_gene_id" + df_not_symbol, + how="right", + left_on="ensembl_gene_id", + right_on="Left_ensembl_gene_id", + ) + df_symbol = hgnc_df.merge( + df_symbol, how="right", left_on="symbol", right_on="GeneA" ) - df_symbol = hgnc_df.merge(df_symbol, how="right", left_on="symbol", right_on="GeneA") df = pd.concat([df_not_symbol, df_symbol]) df = df.rename(columns={"hgnc_id": "Left_hgnc_id"}) @@ -57,20 +62,30 @@ def vcf_collect( df_not_symbol = df[df["Right_ensembl_gene_id"].notna()] df_not_symbol = hgnc_df.merge( - df_not_symbol, how="right", left_on="ensembl_gene_id", right_on="Right_ensembl_gene_id" + df_not_symbol, + how="right", + left_on="ensembl_gene_id", + right_on="Right_ensembl_gene_id", + ) + df_symbol = hgnc_df.merge( + df_symbol, how="right", left_on="symbol", right_on="GeneB" ) - df_symbol = hgnc_df.merge(df_symbol, how="right", left_on="symbol", right_on="GeneB") df = pd.concat([df_not_symbol, df_symbol]) df = df.rename(columns={"hgnc_id": "Right_hgnc_id"}) gtf_df = build_gtf_dataframe(gtf) - all_df = df.merge(gtf_df, how="left", left_on="CDS_LEFT_ID", right_on="Transcript_id") + all_df = df.merge( + gtf_df, how="left", left_on="CDS_LEFT_ID", right_on="Transcript_id" + ) all_df[["PosA", "orig_start", "orig_end"]] = ( all_df[["PosA", "orig_start", "orig_end"]].fillna(0).astype(int) ) all_df = all_df[ - ((all_df["PosA"] >= all_df["orig_start"]) & (all_df["PosA"] <= all_df["orig_end"])) + ( + (all_df["PosA"] >= all_df["orig_start"]) + & (all_df["PosA"] <= all_df["orig_end"]) + ) | ((all_df["orig_start"] == 0) & (all_df["orig_end"] == 0)) ] @@ -84,9 +99,9 @@ def vcf_collect( all_df["exon_number"] = all_df.groupby("PosA")["exon_number"].transform( lambda x: x.fillna(method="ffill").fillna(method="bfill") ) - all_df["transcript_version"] = all_df.groupby("PosA")["transcript_version"].transform( - lambda x: x.fillna(method="ffill").fillna(method="bfill") - ) + all_df["transcript_version"] = all_df.groupby("PosA")[ + "transcript_version" + ].transform(lambda x: x.fillna(method="ffill").fillna(method="bfill")) all_df = all_df.rename(columns={"transcript_version": "Left_transcript_version"}) all_df = all_df.rename(columns={"exon_number": "Left_exon_number"}) @@ -119,15 +134,20 @@ def vcf_collect( ] ].drop_duplicates() all_df["CDS_RIGHT_ID"] = all_df["CDS_RIGHT_ID"].astype("str") - all_df = all_df.merge(gtf_df, how="left", left_on="CDS_RIGHT_ID", right_on="Transcript_id") - all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].fillna( - 0 - ) - all_df[["PosB", "orig_start", "orig_end"]] = all_df[["PosB", "orig_start", "orig_end"]].astype( - int + all_df = all_df.merge( + gtf_df, how="left", left_on="CDS_RIGHT_ID", right_on="Transcript_id" ) + all_df[["PosB", "orig_start", "orig_end"]] = all_df[ + ["PosB", "orig_start", "orig_end"] + ].fillna(0) + all_df[["PosB", "orig_start", "orig_end"]] = all_df[ + ["PosB", "orig_start", "orig_end"] + ].astype(int) all_df = all_df[ - ((all_df["PosB"] >= all_df["orig_start"]) & (all_df["PosB"] <= all_df["orig_end"])) + ( + (all_df["PosB"] >= all_df["orig_start"]) + & (all_df["PosB"] <= all_df["orig_end"]) + ) | ((all_df["orig_start"] == 0) & (all_df["orig_end"] == 0)) ] @@ -141,9 +161,9 @@ def vcf_collect( all_df["exon_number"] = all_df.groupby("PosB")["exon_number"].transform( lambda x: x.fillna(method="ffill").fillna(method="bfill") ) - all_df["transcript_version"] = all_df.groupby("PosB")["transcript_version"].transform( - lambda x: x.fillna(method="ffill").fillna(method="bfill") - ) + all_df["transcript_version"] = all_df.groupby("PosB")[ + "transcript_version" + ].transform(lambda x: x.fillna(method="ffill").fillna(method="bfill")) all_df = all_df.rename(columns={"transcript_version": "Right_transcript_version"}) all_df = all_df.rename(columns={"exon_number": "Right_exon_number"}) @@ -285,16 +305,26 @@ def build_fusioninspector_dataframe(file: str) -> pd.DataFrame: df = pd.read_csv(file, sep="\t") df = df.rename(columns={"#FusionName": "FUSION"}) if not (df.empty): - df[["ChromosomeA", "PosA", "Strand1"]] = df["LeftBreakpoint"].str.split(":", expand=True) - df[["ChromosomeB", "PosB", "Strand2"]] = df["RightBreakpoint"].str.split(":", expand=True) - df[["LeftGeneName", "Left_ensembl_gene_id"]] = df["LeftGene"].str.split("^", expand=True) - df[["RightGeneName", "Right_ensembl_gene_id"]] = df["RightGene"].str.split("^", expand=True) + df[["ChromosomeA", "PosA", "Strand1"]] = df["LeftBreakpoint"].str.split( + ":", expand=True + ) + df[["ChromosomeB", "PosB", "Strand2"]] = df["RightBreakpoint"].str.split( + ":", expand=True + ) + df[["LeftGeneName", "Left_ensembl_gene_id"]] = df["LeftGene"].str.split( + "^", expand=True + ) + df[["RightGeneName", "Right_ensembl_gene_id"]] = df["RightGene"].str.split( + "^", expand=True + ) df["annots"] = ( df["annots"] .apply(convert_to_list) .apply( lambda x: ( - ",".join(map(str, x)) if isinstance(x, list) else str(x) if pd.notna(x) else "" + ",".join(map(str, x)) + if isinstance(x, list) + else str(x) if pd.notna(x) else "" ) ) ) @@ -320,7 +350,9 @@ def build_fusioninspector_dataframe(file: str) -> pd.DataFrame: return df.set_index(["FUSION"]) -def replace_value_with_column_name(row: pd.Series, value_to_replace: str, column_name: str) -> str: +def replace_value_with_column_name( + row: pd.Series, value_to_replace: str, column_name: str +) -> str: """ Replace a specific value in a row with the corresponding column name. """ @@ -350,9 +382,11 @@ def read_build_fusionreport(fusionreport_file: str) -> pd.DataFrame: Make all column headers uppercase. """ with open(fusionreport_file) as f: - from_html = [line.split('rows": ')[1] for line in f if 'name="fusion_list' in line] - tmp=str(from_html)[2:] - tmp2 = (tmp.split(', "tools": ')[0]) + from_html = [ + line.split('rows": ')[1] for line in f if 'name="fusion_list' in line + ] + tmp = str(from_html)[2:] + tmp2 = tmp.split(', "tools": ')[0] fusion_report = pd.DataFrame(ast.literal_eval(tmp2)) if not "arriba" in fusion_report.columns: fusion_report["arriba"] = "" @@ -369,12 +403,16 @@ def read_build_fusionreport(fusionreport_file: str) -> pd.DataFrame: fusion_report["starfusion"] = fusion_report[["starfusion"]].apply( replace_value_with_column_name, args=("true", "starfusion"), axis=1 ) - fusion_report["FOUND_IN"] = fusion_report[["arriba", "starfusion", "fusioncatcher"]].apply( - concatenate_columns, axis=1 - ) + fusion_report["FOUND_IN"] = fusion_report[ + ["arriba", "starfusion", "fusioncatcher"] + ].apply(concatenate_columns, axis=1) fusion_report.columns = fusion_report.columns.str.upper() - fusion_report["FOUND_DB"] = fusion_report["FOUND_DB"].apply(lambda x: ",".join(x) if len(x) > 0 else '') - fusion_report[["GeneA", "GeneB"]] = fusion_report["FUSION"].str.split("--", expand=True) + fusion_report["FOUND_DB"] = fusion_report["FOUND_DB"].apply( + lambda x: ",".join(x) if len(x) > 0 else "" + ) + fusion_report[["GeneA", "GeneB"]] = fusion_report["FUSION"].str.split( + "--", expand=True + ) return fusion_report[ ["FUSION", "GeneA", "GeneB", "TOOLS_HITS", "SCORE", "FOUND_DB", "FOUND_IN"] @@ -418,7 +456,16 @@ def read_fusionreport_csv(file: str) -> pd.DataFrame: df = df.set_index("Fusion") df.to_csv("tmp.csv") return df[ - ["GeneA", "GeneB", "ChromosomeA", "PosA", "StrandA", "ChromosomeB", "PosB", "StrandB"] + [ + "GeneA", + "GeneB", + "ChromosomeA", + "PosA", + "StrandA", + "ChromosomeB", + "PosB", + "StrandB", + ] ] @@ -445,7 +492,9 @@ def column_manipulation(df: pd.DataFrame) -> pd.DataFrame: df["Right_hgnc_id"] = df["Right_hgnc_id"].fillna(0).astype(int).astype(str) df["Left_exon_number"] = df["Left_exon_number"].fillna(0).astype(int).astype(str) df["Right_exon_number"] = df["Right_exon_number"].fillna(0).astype(int).astype(str) - df["Left_transcript_version"] = df["Left_transcript_version"].fillna(0).astype(int).astype(str) + df["Left_transcript_version"] = ( + df["Left_transcript_version"].fillna(0).astype(int).astype(str) + ) df["Right_transcript_version"] = ( df["Right_transcript_version"].fillna(0).astype(int).astype(str) ) @@ -499,7 +548,9 @@ def write_vcf(df_to_print: pd.DataFrame, header: str, out_file: str) -> None: "FORMAT", "Sample", ] - ].to_csv(path_or_buf=out_file, sep="\t", header=None, index=False, quoting=csv.QUOTE_NONE) + ].to_csv( + path_or_buf=out_file, sep="\t", header=None, index=False, quoting=csv.QUOTE_NONE + ) with open(out_file, "r+") as f: content = f.read() @@ -521,11 +572,15 @@ def build_gtf_dataframe(file: str) -> pd.DataFrame: Build a DataFrame from GTF file converted in TSV, extracting relevant columns. """ df = pd.read_csv(file, sep="\t") - df[["fusion_dump", "Transcript_id"]] = df["transcript_id"].str.split("^", expand=True) - df[["orig_chromosome", "orig_start", "orig_end", "orig_dir"]] = df["orig_coord_info"].str.split( - ",", expand=True + df[["fusion_dump", "Transcript_id"]] = df["transcript_id"].str.split( + "^", expand=True ) - return df[["Transcript_id", "transcript_version", "exon_number", "orig_start", "orig_end"]] + df[["orig_chromosome", "orig_start", "orig_end", "orig_dir"]] = df[ + "orig_coord_info" + ].str.split(",", expand=True) + return df[ + ["Transcript_id", "transcript_version", "exon_number", "orig_start", "orig_end"] + ] def main(argv=None): From 2b6ff48b95e7b471fb7c5559e2a0a9ea1f8d91ec Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Tue, 19 Mar 2024 13:44:48 +0100 Subject: [PATCH 52/64] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 58e8c618..5ce3a28a 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -16,6 +16,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - fix bug when using parameter "whitelist" [#466](https://github.com/nf-core/rnafusion/pull/466) - fix VCF_COLLECT handling when a tool is absent from FUSIONREPORT report [#458](https://github.com/nf-core/rnafusion/pull/458) - fix VCF_COLLECT when fusioninspector output is empty but fusionreport is not [#465](https://github.com/nf-core/rnafusion/pull/465) +- fix VCF_COLLECT bug [#481](https://github.com/nf-core/rnafusion/pull/481) ### Removed From 78a56b37cd276a08c8197162c55b2b586543be04 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Wed, 3 Apr 2024 12:14:38 +0200 Subject: [PATCH 53/64] align { --- modules/local/picard/collectrnaseqmetrics/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/picard/collectrnaseqmetrics/main.nf b/modules/local/picard/collectrnaseqmetrics/main.nf index e633e563..ede593e0 100644 --- a/modules/local/picard/collectrnaseqmetrics/main.nf +++ b/modules/local/picard/collectrnaseqmetrics/main.nf @@ -38,7 +38,7 @@ process PICARD_COLLECTRNASEQMETRICS { log.info '[Picard CollectRnaMetrics] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { avail_mem = (task.memory.mega*0.8).intValue() - } + } """ picard \\ -Xmx${avail_mem}M \\ From 3ab9344cbf6b38e2e3d02d53b293010321304e3d Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Wed, 3 Apr 2024 12:24:20 +0200 Subject: [PATCH 54/64] fix logical gate: when stringtie and not starfusion, only alignment is done --- subworkflows/local/starfusion_workflow.nf | 11 ++++++----- 1 file changed, 6 insertions(+), 5 deletions(-) diff --git a/subworkflows/local/starfusion_workflow.nf b/subworkflows/local/starfusion_workflow.nf index ec7a832c..de99d8e5 100644 --- a/subworkflows/local/starfusion_workflow.nf +++ b/subworkflows/local/starfusion_workflow.nf @@ -30,6 +30,7 @@ workflow STARFUSION_WORKFLOW { SAMTOOLS_INDEX_FOR_STARFUSION(STAR_FOR_STARFUSION.out.bam_sorted) ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_STARFUSION.out.versions) bam_sorted_indexed = STAR_FOR_STARFUSION.out.bam_sorted.join(SAMTOOLS_INDEX_FOR_STARFUSION.out.bai) + reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction ) if (params.cram.contains('starfusion')){ SAMTOOLS_VIEW_FOR_STARFUSION (bam_sorted_indexed, ch_fasta, [] ) @@ -38,12 +39,12 @@ workflow STARFUSION_WORKFLOW { SAMTOOLS_INDEX_FOR_STARFUSION_CRAM (SAMTOOLS_VIEW_FOR_STARFUSION.out.cram) ch_versions = ch_versions.mix(SAMTOOLS_INDEX_FOR_STARFUSION_CRAM.out.versions) } - reads_junction = reads.join(STAR_FOR_STARFUSION.out.junction ) - - STARFUSION( reads_junction, params.starfusion_ref) - ch_versions = ch_versions.mix(STARFUSION.out.versions) + if (params.starfusion || params.all){ + STARFUSION( reads_junction, params.starfusion_ref) + ch_versions = ch_versions.mix(STARFUSION.out.versions) + ch_starfusion_fusions = STARFUSION.out.fusions + } - ch_starfusion_fusions = STARFUSION.out.fusions ch_star_stats = STAR_FOR_STARFUSION.out.log_final ch_star_gene_count = STAR_FOR_STARFUSION.out.read_per_gene_tab } From 6a36c3ebb64ffb98a0fc8b19c68355c6ce8d0a44 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Wed, 3 Apr 2024 12:39:46 +0200 Subject: [PATCH 55/64] fix conda package requirements --- modules/local/starfusion/detect/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/starfusion/detect/main.nf b/modules/local/starfusion/detect/main.nf index fba671b6..1994d555 100644 --- a/modules/local/starfusion/detect/main.nf +++ b/modules/local/starfusion/detect/main.nf @@ -2,7 +2,7 @@ process STARFUSION { tag "$meta.id" label 'process_high' - conda "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.12.0 bioconda::=2.13.2 bioconda::samtools=1.9 bioconda::star=2.7.8a" + conda "bioconda::dfam=3.3 bioconda::hmmer=3.3.2 bioconda::star-fusion=1.12.0 bioconda::trinity=2.13.2 bioconda::samtools=1.9 bioconda::star=2.7.8a" container 'docker.io/trinityctat/starfusion:1.12.0' input: From d506c69ea33358c5995e1d992a6e670084f280ac Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Wed, 3 Apr 2024 12:49:26 +0200 Subject: [PATCH 56/64] update changelog --- CHANGELOG.md | 2 ++ 1 file changed, 2 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 5ce3a28a..4f09369c 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -17,6 +17,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - fix VCF_COLLECT handling when a tool is absent from FUSIONREPORT report [#458](https://github.com/nf-core/rnafusion/pull/458) - fix VCF_COLLECT when fusioninspector output is empty but fusionreport is not [#465](https://github.com/nf-core/rnafusion/pull/465) - fix VCF_COLLECT bug [#481](https://github.com/nf-core/rnafusion/pull/481) +- fix conda package for starfusion/detect[#482](https://github.com/nf-core/rnafusion/pull/482) +- fix logical gate so when stringtie should run but not starfusion, starfusion will not run[#482](https://github.com/nf-core/rnafusion/pull/482) ### Removed From e3a909f6b43d5517d73f907d707bdce07134f389 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Wed, 3 Apr 2024 16:06:46 +0200 Subject: [PATCH 57/64] bump version 3.0.2 --- assets/multiqc_config.yml | 4 ++-- nextflow.config | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 963555cf..fe045988 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/rnafusion + This report has been generated by the nf-core/rnafusion analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: nf-core-rnafusion-methods-description: diff --git a/nextflow.config b/nextflow.config index 1a35e3c7..23a39b21 100644 --- a/nextflow.config +++ b/nextflow.config @@ -301,7 +301,7 @@ manifest { description = """Nextflow rnafusion analysis pipeline, part of the nf-core community.""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '3.0.2dev' + version = '3.0.2' doi = '' } From 2dbd1346401ff131b74269fd64e1932f16384e72 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Wed, 3 Apr 2024 16:18:44 +0200 Subject: [PATCH 58/64] update changelog date --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index c19e02a8..d0bb6bfb 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v3.0.2dev - [date] +## v3.0.2 - [2024-04-03] ### Added From 58dd1129ec03aebf1c7be0e96b9ad78f2b48b5aa Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 8 Apr 2024 10:52:17 +0200 Subject: [PATCH 59/64] remove emtpy file --- 512 | 0 1 file changed, 0 insertions(+), 0 deletions(-) delete mode 100644 512 diff --git a/512 b/512 deleted file mode 100644 index e69de29b..00000000 From a5428bf456cf3bf46ec22a8265abae67754221b6 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 8 Apr 2024 13:26:24 +0200 Subject: [PATCH 60/64] Update nextflow.config Co-authored-by: Felix Lenner <52530259+fellen31@users.noreply.github.com> --- nextflow.config | 14 +++++++------- 1 file changed, 7 insertions(+), 7 deletions(-) diff --git a/nextflow.config b/nextflow.config index 23a39b21..5163dab2 100644 --- a/nextflow.config +++ b/nextflow.config @@ -32,13 +32,13 @@ params { genomes = [:] // Genomes options - fasta = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa" - fai = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa.fai" - gtf = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.gtf" - chrgtf = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf" - transcript = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.cdna.all.fa.gz" - refflat = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf.refflat" - rrna_intervals= "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.interval_list" + fasta = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa" + fai = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.all.fa.fai" + gtf = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.gtf" + chrgtf = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf" + transcript = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.cdna.all.fa.gz" + refflat = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.chr.gtf.refflat" + rrna_intervals = "${params.genomes_base}/ensembl/Homo_sapiens.${params.genome}.${params.ensembl_version}.interval_list" // Filtering tools_cutoff = 1 From 3602cc0b9b6c5c2aa6f2d71306fae06b16bba0da Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 8 Apr 2024 16:49:46 +0200 Subject: [PATCH 61/64] remove nf-test.log --- .nf-test.log | 4 ---- 1 file changed, 4 deletions(-) delete mode 100644 .nf-test.log diff --git a/.nf-test.log b/.nf-test.log deleted file mode 100644 index 8da6769a..00000000 --- a/.nf-test.log +++ /dev/null @@ -1,4 +0,0 @@ -Mar-06 08:33:23.861 [main] INFO com.askimed.nf.test.App - nf-test 0.8.1 -Mar-06 08:33:23.919 [main] INFO com.askimed.nf.test.App - Arguments: [test, --profile, debug,test,docker, --verbose] -Mar-06 08:33:23.924 [main] INFO com.askimed.nf.test.commands.RunTestsCommand - Load config from file /Users/annick.renevey/Projects/rnafusion/nf-test.config... -Mar-06 08:33:24.984 [main] ERROR com.askimed.nf.test.commands.RunTestsCommand - No tests ot directories found containing test files. From 5adb5216b9f3078b5bfdfcd0bdf0aa831f128e89 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 8 Apr 2024 16:50:17 +0200 Subject: [PATCH 62/64] Update workflows/rnafusion.nf Co-authored-by: Felix Lenner <52530259+fellen31@users.noreply.github.com> --- workflows/rnafusion.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/workflows/rnafusion.nf b/workflows/rnafusion.nf index 511b121b..7483f1be 100644 --- a/workflows/rnafusion.nf +++ b/workflows/rnafusion.nf @@ -132,7 +132,6 @@ workflow RNAFUSION { // // SUBWORKFLOW: Run STAR alignment and Arriba // - ARRIBA_WORKFLOW ( ch_reads_all, ch_gtf, From d09c5bbdd736b7f1c12816b7930e587b7eee93d7 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Mon, 8 Apr 2024 16:51:58 +0200 Subject: [PATCH 63/64] use a real input as validation of the samplesheet occurs even with build_references, althought the main detection rnafusion analysis will not run --- nextflow.config | 3 ++- subworkflows/local/fusioninspector_workflow.nf | 2 -- 2 files changed, 2 insertions(+), 3 deletions(-) diff --git a/nextflow.config b/nextflow.config index 23a39b21..7bc32137 100644 --- a/nextflow.config +++ b/nextflow.config @@ -10,7 +10,8 @@ params { // Input options - input = "fake_input_to_build_refs.csv" + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv' + build_references = false cosmic_username = null cosmic_passwd = null diff --git a/subworkflows/local/fusioninspector_workflow.nf b/subworkflows/local/fusioninspector_workflow.nf index a826fbfc..f521ae8c 100644 --- a/subworkflows/local/fusioninspector_workflow.nf +++ b/subworkflows/local/fusioninspector_workflow.nf @@ -29,8 +29,6 @@ workflow FUSIONINSPECTOR_WORKFLOW { fusions: it[1].size() > 0 } - ch_fusion_list.fusions.dump(tag:'before_cat') - if (params.whitelist) { ch_whitelist = ch_fusion_list.fusions.combine(Channel.value(file(params.whitelist, checkIfExists:true))) .map { meta, fusions, whitelist -> [ meta, [fusions, whitelist] ] } From c27117b0c3e49be715ad7346cf7d2d373d9342b3 Mon Sep 17 00:00:00 2001 From: Annick Renevey <47788523+rannick@users.noreply.github.com> Date: Wed, 10 Apr 2024 10:14:03 +0200 Subject: [PATCH 64/64] final release date in changelog --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index d0bb6bfb..871c4659 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v3.0.2 - [2024-04-03] +## v3.0.2 - [2024-04-10] ### Added
    Process Name \\", - " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
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Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } } diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar deleted file mode 100644 index 805c8bb5e4fd43a12a5891eea5a68788309629b0..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 2291171 zcma%i1CS<7mu}m(-Ea4_?dhK8v~AnAZQHhO+qP}n-E-&Lf8*Yb{bM(7MpZ;s){{|L znGpxiIY(9k6buar3JMCSNkdW|=)W{*ATS^)VMTsgF=-L{?{Od?P#{?eNT`1hApZ%I z{U0Zz{YCuW$x{5%Vj{u{igZ#U4^op8k`lCZbMO+hRMV3)4e|_&%)3YSbmCGpVp4NX zni_mKLGakt&P12{r^KCD0GPa9sIuy`fu+agt?8O-hY9i{WloGe}y@kSsDEY_Fud9 z-?2ab4J)MQXe4E3Xl-U<>PRMQZ)0m@@90KnV5#Te5Tz`pvZ0Lj4F<9%Flh(`Qb;Tn zMV!iFmH{S^fF*j#MCKRUd`7m>9||*;WE32I_6pg4-Q1C#ke$$aVmP-TtfJ+j+N9;Q z>bxN=bCVQkg$Jzty71(+~A9}ZfANuYIVnTdW!??Sj)Ti-Qpk8XgPDab8pk2QD&dwUl=iFhd1c$C2uMVagr zWc@z9s$#g__{3?VGb#)^fSNp^lZh zU>MI!D?+L)nyth{@@L0+B&_M8iR08L)G6#PZQLFmvz-9DlZkwRWigRIyI4xYr7>tRJ-N6MlBB`nJQ!!8D)mI2lB3PP;dC{<{Td>^ 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