diff --git a/Dockerfile b/Dockerfile index a04c03ce..303dd2c1 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,8 +1,8 @@ FROM nfcore/base MAINTAINER Phil Ewels LABEL authors="phil.ewels@scilifelab.se" \ - description="Docker image containing all requirements for the nfcore/ChIPseq pipeline" + description="Docker image containing all requirements for the nfcore/ChIPseq pipeline" COPY environment.yml / RUN conda env create -f /environment.yml && conda clean -a -ENV PATH /opt/conda/envs/nfcore-chipseq-1.4dev/bin:$PATH +ENV PATH /opt/conda/envs/nfcore-chipseq-1.4dev/bin:$PATH \ No newline at end of file diff --git a/environment.yml b/environment.yml index e7d4bef6..6263efe9 100644 --- a/environment.yml +++ b/environment.yml @@ -10,6 +10,7 @@ dependencies: - fastqc=0.11.7 - trim-galore=0.4.5 - bwa=0.7.17 + - conda-forge::gawk=4.2.1 - conda-forge::r-markdown=0.8 - bioconda::r-spp=1.15.2 - bedtools=2.27.1 @@ -28,6 +29,7 @@ dependencies: - conda-forge::r-ggplot2=2.2.1 - conda-forge::r-knitr=1.20 - conda-forge::r-rmysql=0.10.13 + - conda-forge::r-doparallel=1.0.11 - bioconda::bioconductor-bsgenome=1.46.0 - bioconda::bioconductor-rsamtools=1.30.0 - bioconda::bioconductor-shortread=1.36.0 @@ -41,4 +43,5 @@ dependencies: - bioconda::bioconductor-org.hs.eg.db=3.5.0 - bioconda::bioconductor-bsgenome.mmusculus.ucsc.mm10=1.4.0 - bioconda::bioconductor-org.mm.eg.db=3.5.0 - - bioconda::r-spp=1.15.2 \ No newline at end of file + - deeptools=3.0.2 + - bioconda::r-spp=1.15.2 diff --git a/main.nf b/main.nf index f61b9daf..ceed11ff 100755 --- a/main.nf +++ b/main.nf @@ -597,7 +597,7 @@ process deepTools { bamCoverage \\ -b $bam \\ --extendReads ${params.extendReadsLen} \\ - --normalizeUsingRPKM \\ + --normalizeUsing RPKM \\ -o ${bam}.bw """ } else { @@ -619,7 +619,7 @@ process deepTools { bamCoverage \\ -b \$bamfile \\ --extendReads ${params.extendReadsLen} \\ - --normalizeUsingRPKM \\ + --normalizeUsing RPKM \\ -o \${bamfile}.bw done