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Bakta running only for one sample #171

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SchwarzMarek opened this issue Oct 1, 2024 · 5 comments
Closed

Bakta running only for one sample #171

SchwarzMarek opened this issue Oct 1, 2024 · 5 comments
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@SchwarzMarek
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Description of the bug

When running bacass for multiple samples, bakta output is produced only for one of them.
Execution trace and sw version are included in zip. (no .nextflow.log for this one, sorry)

Command used and terminal output

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Relevant files

pipeline_info.zip

System information

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@SchwarzMarek SchwarzMarek added the bug Something isn't working label Oct 1, 2024
@Daniel-VM Daniel-VM self-assigned this Oct 15, 2024
@Daniel-VM
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Hi @SchwarzMarek ,

I have ran -r dev and no issues... Can you try to reproduce this bug?

nextflow run main.nf -profile test,docker --annotation_tool bakta --baktadb_download true --outdir results

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@SchwarzMarek
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Hi,
still no luck...

.nextflow.log

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@Daniel-VM
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It seems something went wrong with the assembly step in sample ERR044595. Was the FASTA file generated correctly? There is no value under the # contig column for this sample.

@SchwarzMarek
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I can't see anything wrong with the assembly step. I noticed this issue when I wanted more precise annotation than those provided by prokka and did rerun with bakta. With prokka, I had annotations for every sample. One thing to note is that I was using full bakta db then (and also for this test data here).

I'm uploading the full multiqc report. multiqc.zip

Best
Marek

@Daniel-VM
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Got it! Thanks for testing it out. If possible, please review it here: #178

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