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I have aligned/basecalled pod5->bams that I want to demultiplex into two files based on the presence/absence of a specific adapter sequence at the beginning of the read. Can I use dorado demux with custom barcodes to do this? When I read the documentation it seems to support demuxing based on barcodes flanked by constant sequences, but I'm not sure if it's capable of doing something like presence/absence of a specific sequence.
Run environment:
Dorado version: v0.8.0
Dorado command: dorado demux
Operating system: Linux
Hardware (CPUs, Memory, GPUs): A100/H100
Source data type (e.g., pod5 or fast5 - please note we always recommend converting to pod5 for optimal basecalling performance): pod5
The text was updated successfully, but these errors were encountered:
No, dorado does not have any specific facility to demux based on the adapters. You could try just aligning to the adapter sequences. Alternatively, if the adapter is long enough you could split it into two or three sections and use these as the mask and a single barcode for a custom barcode arrangement, but this is untested.
Issue Report
Please describe the issue:
I have aligned/basecalled pod5->bams that I want to demultiplex into two files based on the presence/absence of a specific adapter sequence at the beginning of the read. Can I use dorado demux with custom barcodes to do this? When I read the documentation it seems to support demuxing based on barcodes flanked by constant sequences, but I'm not sure if it's capable of doing something like presence/absence of a specific sequence.
Run environment:
The text was updated successfully, but these errors were encountered: