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Profile long reads

Alessio Milanese edited this page Jul 12, 2022 · 16 revisions

Since mOTUs 3.0.3 it is possible to profile long reads with mOTUs.

Given a long read fasta file (let's say long_reads.fasta), you need to first run:

motus prep_long -i long_reads.fasta -o converted_long_reads.fasta.gz

to split the long reads into shorter reads that can be profiled by a default mOTUs profile call like:

motus profile -s converted_long_reads.fasta.gz

Note that the command prep_long works on both fasta and fastq files, as well as on .gz files.