Exome vs RNAseq #1074
Exome vs RNAseq
#1074
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We happen to have (bulk) exome and RNAseq on the same samples and I was curious whether people find one or the other is better for mixcr? We plan to try both and compare, but that's just one dataset. |
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Answered by
PoslavskySV
Apr 17, 2023
Replies: 1 comment
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As individual TCRs/BCRs are represented in RNA level by several (lots) of transcripts in comparison to DNA level (only one rearrangement) there is higher probability to extract/assemble CDR3 region from RNAseq. So RNA-Seq is definitely better. |
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PoslavskySV
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As individual TCRs/BCRs are represented in RNA level by several (lots) of transcripts in comparison to DNA level (only one rearrangement) there is higher probability to extract/assemble CDR3 region from RNAseq. So RNA-Seq is definitely better.