This package is a modified version of Nick Sofroniew's 2P-Mesoscope PSF analysis package. (https://github.com/sofroniewn/psf)
Compute the point spread function from a 3d image of beads
This package computes the point spread function (psf) from a 3d image of beads. It first finds the centers of each well separated bead and then fits 2d Gaussians to max projections of the bead. It returns the resulting psfs as a table. Look at the examply jupyter notebook for usage instructions.
git clone https://github.com/mesoSPIM/mesoSPIM-PSFanalysis