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Nikita Vladimirov edited this page Jan 27, 2022
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Just like its predecessor, mesoSPIM-V5, BT-mesoSPIM is a versatile facility-grade lightsheet microscope for imaging cleared tissues.
- compatibility with all clearing techniques including CLARITY, CUBIC, and iDISCO
- dual-sided axially scanned lightsheet excitation for uniform optical sectioning across entire field of view
- large field of view, from 10 up to 43 mm using commercially available telecentric lenses and new-generation sCMOS cameras
- motorized stages with long travel range (50x50x100 mm) and sub-micron precision, suitable for large samples.
- resolution up to 2 µm in XY and 6 µm in Z
- open-source hardware and software, open for modifications and upgrades
- compact system dimensions: 450x450x600 mm.
TBD from BOM
- Overview
- Room requirements
- Safety
- Parts list
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Assembly
- Overview
- Detection arm
- Excitation arms
- Sample stages
- Electronics
- Lasers
- Immersion chambers (TODO)
- Front cover (with webcam)
- Enclosure (TODO)
- Usage
- Troubleshooting and typical errors (TODO)
- Maintenance(TODO)
- Technical notes