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Strange hapmer result #151

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YPGG1234 opened this issue Dec 30, 2024 · 1 comment
Closed

Strange hapmer result #151

YPGG1234 opened this issue Dec 30, 2024 · 1 comment

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@YPGG1234
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YPGG1234 commented Dec 30, 2024

Thank you for developing such an excellent tool! I'm now testing it using results from two sets of triobin

$meryl k=21 count ${pat_dir}/*fq.gz output pat.meryl # ngs
$meryl k=21 count ${mat_dir}/*fq.gz output mat.meryl # ngs
$meryl k=21 count ${chi_dir}/*fq.gz output chi.meryl # ngs
$hapmer mat.meryl pat.meryl chi.meryl
###$merqury chi.meryl mat.hapmer.meryl pat.hapmer.meryl $hap2 $hap1 output

But our results seem a little strange, just like #147 It seems that the curves of child-only and mat are reversed.
The first triobin
first
The second triobin
second

Here is the part of output of the first triobin: output.hap1.genome.pat.hapmer.spectra-cn.ln.png and output.hap1.genome.mat.hapmer.spectra-cn.ln.png
output hap1 genome pat hapmer spectra-cn ln
output hap1 genome mat hapmer spectra-cn ln

We think we're using the right two sets of triobin, but why is this happening? Looking forward to your reply

@YPGG1234
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YPGG1234 commented Jan 1, 2025

I finally solved the problem by changing the kmer

@YPGG1234 YPGG1234 closed this as completed Jan 1, 2025
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