From 27e4cb3cf1d3c8e197ae8b353389b39862132224 Mon Sep 17 00:00:00 2001
From: Tao Liu <vladimir.liu@gmail.com>
Date: Tue, 4 Oct 2022 17:00:18 -0400
Subject: [PATCH] change way to decide the open/nuc/bg states

---
 MACS3/Commands/hmmratac_cmd.py                |   13 +-
 MACS3/Signal/BedGraph.pyx                     |    4 +-
 MACS3/Signal/HMMR_Signal_Processing.pyx       |   14 +-
 ...ac_yeast500k_accessible_regions.gappedPeak | 1438 ++++-------------
 4 files changed, 294 insertions(+), 1175 deletions(-)

diff --git a/MACS3/Commands/hmmratac_cmd.py b/MACS3/Commands/hmmratac_cmd.py
index e980d16c..5300d51b 100644
--- a/MACS3/Commands/hmmratac_cmd.py
+++ b/MACS3/Commands/hmmratac_cmd.py
@@ -1,4 +1,4 @@
-# Time-stamp: <2022-10-04 15:13:57 Tao Liu>
+# Time-stamp: <2022-10-04 16:58:30 Tao Liu>
 
 """Description: Main HMMR command
 
@@ -243,8 +243,15 @@ def run( args ):
         options.info( f"#   HMM converged: {hmm_model.monitor_.converged}")
 
         # label hidden states
-        i_open_region = np.where(hmm_model.means_ == max(hmm_model.means_[0:3,0]))[0][0]
-        i_background_region = np.where(hmm_model.transmat_ == min(hmm_model.transmat_[0:3, i_open_region]))[0][0]
+        means_sum = np.sum( hmm_model.means_, axis=1 )
+
+        # first, the state with the highest overall emission is the open state
+        i_open_region = np.where( means_sum == max(means_sum) )[0][0]
+
+        # second, the state with lowest overall emission is the bg state 
+        i_background_region = np.where( means_sum == min(means_sum) )[0][0]
+
+        # last one is the nuc state (note it may not be accurate though
         i_nucleosomal_region = list(set([0, 1, 2]) - set([i_open_region, i_background_region]))[0]
 
         # write hmm into model file
diff --git a/MACS3/Signal/BedGraph.pyx b/MACS3/Signal/BedGraph.pyx
index f36702d5..edc7e502 100644
--- a/MACS3/Signal/BedGraph.pyx
+++ b/MACS3/Signal/BedGraph.pyx
@@ -1,6 +1,6 @@
 # cython: language_level=3
 # cython: profile=True
-# Time-stamp: <2022-09-29 09:07:23 Tao Liu>
+# Time-stamp: <2022-10-04 15:46:00 Tao Liu>
 
 """Module for BedGraph data class.
 
@@ -981,7 +981,7 @@ cdef class bedGraphTrackI:
              int32_t pre_p, p1, p2, i
              float32_t v1, v2
              bytes chrom
-             object ret
+             list ret
 
         assert isinstance(bdgTrack2,bedGraphTrackI), "not a bedGraphTrackI object"
 
diff --git a/MACS3/Signal/HMMR_Signal_Processing.pyx b/MACS3/Signal/HMMR_Signal_Processing.pyx
index 8f2e2688..b039474f 100644
--- a/MACS3/Signal/HMMR_Signal_Processing.pyx
+++ b/MACS3/Signal/HMMR_Signal_Processing.pyx
@@ -1,6 +1,6 @@
 # cython: language_level=3
 # cython: profile=True
-# Time-stamp: <2022-09-29 14:58:12 Tao Liu>
+# Time-stamp: <2022-10-04 16:14:23 Tao Liu>
 
 """Module description:
 
@@ -155,23 +155,19 @@ cpdef list extract_signals_from_regions( list signals, object regions, int binsi
     assert len( extracted_data[0] ) == len( extracted_data[1] )
     assert len( extracted_data[0] ) == len( extracted_data[2] )
     assert len( extracted_data[0] ) == len( extracted_data[3] )
-    #nnn =len( extracted_len[0] )
-    #debug( f"{n} bins, {nn}, {nnn}" )
     counter = 0
     prev_c = extracted_len[0][0]
     c = 0
     for i in range( nn ):
         ret_training_bins.append( extracted_positions[0][i] )
         ret_training_data.append(
-            [ max( 0.0001, abs(round(extracted_data[0][i], 4))),
-              max( 0.0001, abs(round(extracted_data[1][i], 4))),
-              max( 0.0001, abs(round(extracted_data[2][i], 4))),
-              max( 0.0001, abs(round(extracted_data[3][i], 4))) ] )
+            [ max( 0.0001, extracted_data[0][i] ),
+              max( 0.0001, extracted_data[1][i] ),
+              max( 0.0001, extracted_data[2][i] ),
+              max( 0.0001, extracted_data[3][i] ) ] )
         c = extracted_len[0][i]
-        #print(f"{extracted_positions[0][i]} {extracted_len[0][i]}")
         if counter != 0 and c != prev_c:
             ret_training_lengths.append( counter )
-            #print(f"### add a bin length {counter}")
             counter = 0
         prev_c = c
         counter +=  1
diff --git a/test/standard_results_hmmratac/hmmratac_yeast500k_accessible_regions.gappedPeak b/test/standard_results_hmmratac/hmmratac_yeast500k_accessible_regions.gappedPeak
index d8823385..e4b8ae0a 100644
--- a/test/standard_results_hmmratac/hmmratac_yeast500k_accessible_regions.gappedPeak
+++ b/test/standard_results_hmmratac/hmmratac_yeast500k_accessible_regions.gappedPeak
@@ -1,1162 +1,278 @@
 track name="peak" description="peak" type=gappedPeak nextItemButton=on
-chrI	20630	30810	peak_1	0	.	20700	30050	0	3	440,190,250	70,8020,9170	0	0	0
-chrI	30880	31600	peak_2	0	.	31160	31290	0	1	130	280	0	0	0
-chrI	34930	42930	peak_3	0	.	39110	39300	0	1	190	4180	0	0	0
-chrI	44540	45890	peak_4	0	.	45200	45710	0	1	510	660	0	0	0
-chrI	46360	57420	peak_5	0	.	48350	48600	0	1	250	1990	0	0	0
-chrI	81140	82970	peak_6	0	.	82020	82390	0	1	370	880	0	0	0
-chrI	84580	92930	peak_7	0	.	92410	92780	0	1	370	7830	0	0	0
-chrI	125190	131120	peak_8	0	.	129210	130740	0	3	300,420,220	4020,4780,5330	0	0	0
-chrI	131620	138490	peak_9	0	.	131660	132320	0	2	230,260	40,440	0	0	0
-chrI	138560	139740	peak_10	0	.	139030	139650	0	2	270,110	470,980	0	0	0
-chrI	142250	143840	peak_11	0	.	143450	143610	0	1	160	1200	0	0	0
-chrI	165900	180720	peak_12	0	.	166160	169210	0	2	310,430	260,2880	0	0	0
-chrI	181650	188710	peak_13	0	.	182330	182740	0	1	410	680	0	0	0
-chrI	199140	219740	peak_14	0	.	210900	211330	0	1	430	11760	0	0	0
-chrII	3690	5350	peak_15	0	.	3750	3930	0	1	180	60	0	0	0
-chrII	7240	10620	peak_16	0	.	9590	9710	0	1	120	2350	0	0	0
-chrII	21480	24550	peak_17	0	.	23850	24090	0	1	240	2370	0	0	0
-chrII	28250	30250	peak_18	0	.	28510	28960	0	1	450	260	0	0	0
-chrII	45730	46600	peak_19	0	.	45750	45890	0	1	140	20	0	0	0
-chrII	60510	61280	peak_20	0	.	60820	61120	0	1	300	310	0	0	0
-chrII	62730	75030	peak_21	0	.	63530	74630	0	2	160,310	800,11590	0	0	0
-chrII	75130	84360	peak_22	0	.	80790	80960	0	1	170	5660	0	0	0
-chrII	84490	88300	peak_23	0	.	87740	88050	0	1	310	3250	0	0	0
-chrII	99180	107560	peak_24	0	.	100140	102320	0	2	170,1080	960,2060	0	0	0
-chrII	107770	111070	peak_25	0	.	107840	108060	0	1	220	70	0	0	0
-chrII	117410	127980	peak_26	0	.	124840	125030	0	1	190	7430	0	0	0
-chrII	128310	133010	peak_27	0	.	132220	132360	0	1	140	3910	0	0	0
-chrII	141330	142990	peak_28	0	.	142620	142900	0	1	280	1290	0	0	0
-chrII	143490	146980	peak_29	0	.	146000	146170	0	1	170	2510	0	0	0
-chrII	160770	164790	peak_30	0	.	164190	164430	0	1	240	3420	0	0	0
-chrII	165660	170210	peak_31	0	.	167710	169310	0	3	120,540,310	2050,2230,3340	0	0	0
-chrII	170290	181430	peak_32	0	.	181090	181280	0	1	190	10800	0	0	0
-chrII	186720	193160	peak_33	0	.	191840	192160	0	1	320	5120	0	0	0
-chrII	196620	201790	peak_34	0	.	197270	197860	0	2	320,210	650,1030	0	0	0
-chrII	217420	222530	peak_35	0	.	221290	221860	0	1	570	3870	0	0	0
-chrII	226090	236820	peak_36	0	.	227090	236290	0	2	310,320	1000,9880	0	0	0
-chrII	237430	238360	peak_37	0	.	237530	237650	0	1	120	100	0	0	0
-chrII	248030	251680	peak_38	0	.	251070	251640	0	1	570	3040	0	0	0
-chrII	254850	257960	peak_39	0	.	255850	256210	0	1	360	1000	0	0	0
-chrII	258240	269740	peak_40	0	.	259900	260600	0	1	700	1660	0	0	0
-chrII	270360	300680	peak_41	0	.	280960	298800	0	5	270,350,430,170,300	10600,17860,21640,27500,28140	0	0	0
-chrII	301160	307080	peak_42	0	.	306280	306570	0	1	290	5120	0	0	0
-chrII	308060	310840	peak_43	0	.	310240	310510	0	1	270	2180	0	0	0
-chrII	311670	324170	peak_44	0	.	315380	315510	0	1	130	3710	0	0	0
-chrII	326170	331220	peak_45	0	.	326730	326930	0	1	200	560	0	0	0
-chrII	331670	336330	peak_46	0	.	332460	332760	0	1	300	790	0	0	0
-chrII	339370	341340	peak_47	0	.	339440	340950	0	2	250,140	70,1440	0	0	0
-chrII	342620	350750	peak_48	0	.	347510	347770	0	1	260	4890	0	0	0
-chrII	355060	370850	peak_49	0	.	356060	369930	0	5	120,180,120,150,150	1000,1560,5350,7610,14720	0	0	0
-chrII	371830	372810	peak_50	0	.	372490	372720	0	1	230	660	0	0	0
-chrII	374920	377730	peak_51	0	.	375890	377000	0	2	460,320	970,1760	0	0	0
-chrII	378240	381120	peak_52	0	.	378680	380140	0	2	370,160	440,1740	0	0	0
-chrII	381140	391540	peak_53	0	.	381360	381650	0	1	290	220	0	0	0
-chrII	393410	394500	peak_54	0	.	393460	393570	0	1	110	50	0	0	0
-chrII	395620	405430	peak_55	0	.	400930	401100	0	1	170	5310	0	0	0
-chrII	405520	415310	peak_56	0	.	405800	410950	0	4	410,230,690,300	280,1820,2790,5130	0	0	0
-chrII	416000	419100	peak_57	0	.	418180	418510	0	1	330	2180	0	0	0
-chrII	427650	435780	peak_58	0	.	429320	429450	0	1	130	1670	0	0	0
-chrII	435860	451260	peak_59	0	.	444580	445200	0	1	620	8720	0	0	0
-chrII	451480	452150	peak_60	0	.	451720	451850	0	1	130	240	0	0	0
-chrII	455730	461620	peak_61	0	.	458430	458940	0	1	510	2700	0	0	0
-chrII	461630	465990	peak_62	0	.	461950	462080	0	1	130	320	0	0	0
-chrII	468000	474720	peak_63	0	.	474060	474270	0	1	210	6060	0	0	0
-chrII	476870	477650	peak_64	0	.	477110	477490	0	1	380	240	0	0	0
-chrII	479000	483060	peak_65	0	.	479150	479260	0	1	110	150	0	0	0
-chrII	483380	488870	peak_66	0	.	483560	486860	0	2	290,290	180,3190	0	0	0
-chrII	491170	495400	peak_67	0	.	491610	493230	0	3	130,280,210	440,1290,1850	0	0	0
-chrII	501420	539800	peak_68	0	.	501660	533630	0	4	170,470,150,280	240,2990,30890,31930	0	0	0
-chrII	560860	561250	peak_69	0	.	561040	561210	0	1	170	180	0	0	0
-chrII	561310	564840	peak_70	0	.	561410	561520	0	1	110	100	0	0	0
-chrII	575530	576350	peak_71	0	.	576120	576270	0	1	150	590	0	0	0
-chrII	578250	581790	peak_72	0	.	581460	581610	0	1	150	3210	0	0	0
-chrII	583610	593770	peak_73	0	.	586260	593120	0	3	220,700,290	2650,5300,9220	0	0	0
-chrII	595220	596750	peak_74	0	.	595620	595920	0	1	300	400	0	0	0
-chrII	603430	605260	peak_75	0	.	604060	604520	0	1	460	630	0	0	0
-chrII	605420	609450	peak_76	0	.	605800	606350	0	1	550	380	0	0	0
-chrII	609520	612790	peak_77	0	.	612510	612630	0	1	120	2990	0	0	0
-chrII	616200	641650	peak_78	0	.	618320	628840	0	3	710,880,340	2120,8570,12300	0	0	0
-chrII	643200	650660	peak_79	0	.	644890	650230	0	3	660,470,120	1690,3850,6910	0	0	0
-chrII	662230	662990	peak_80	0	.	662530	662880	0	1	350	300	0	0	0
-chrII	679350	680860	peak_81	0	.	679950	680830	0	2	620,150	600,1330	0	0	0
-chrII	683400	697380	peak_82	0	.	686680	686920	0	1	240	3280	0	0	0
-chrII	703140	707300	peak_83	0	.	703490	706950	0	2	830,110	350,3700	0	0	0
-chrII	707380	720760	peak_84	0	.	718220	718610	0	1	390	10840	0	0	0
-chrII	723210	724180	peak_85	0	.	723610	723760	0	1	150	400	0	0	0
-chrII	741640	760260	peak_86	0	.	742610	742730	0	1	120	970	0	0	0
-chrII	761330	768070	peak_87	0	.	767650	767760	0	1	110	6320	0	0	0
-chrII	774240	776260	peak_88	0	.	775730	776200	0	1	470	1490	0	0	0
-chrII	785890	805550	peak_89	0	.	786880	805210	0	3	190,220,210	990,13000,19110	0	0	0
-chrIII	990	24050	peak_90	0	.	16990	17260	0	1	270	16000	0	0	0
-chrIII	24730	31260	peak_91	0	.	29110	30700	0	2	270,150	4380,5820	0	0	0
-chrIII	40820	47940	peak_92	0	.	47010	47350	0	1	340	6190	0	0	0
-chrIII	49930	50340	peak_93	0	.	50030	50250	0	1	220	100	0	0	0
-chrIII	62360	63030	peak_94	0	.	62400	62740	0	1	340	40	0	0	0
-chrIII	64010	68460	peak_95	0	.	65590	65750	0	1	160	1580	0	0	0
-chrIII	70050	76950	peak_96	0	.	71510	71700	0	1	190	1460	0	0	0
-chrIII	77860	78890	peak_97	0	.	78030	78700	0	1	670	170	0	0	0
-chrIII	79210	91320	peak_98	0	.	85160	91170	0	3	110,360,390	5950,6090,11570	0	0	0
-chrIII	95740	102810	peak_99	0	.	95850	96050	0	1	200	110	0	0	0
-chrIII	103360	103900	peak_100	0	.	103420	103750	0	1	330	60	0	0	0
-chrIII	124400	125450	peak_101	0	.	125050	125320	0	1	270	650	0	0	0
-chrIII	127980	133340	peak_102	0	.	131610	131760	0	1	150	3630	0	0	0
-chrIII	142240	156110	peak_103	0	.	142680	142840	0	1	160	440	0	0	0
-chrIII	156170	156850	peak_104	0	.	156220	156570	0	1	350	50	0	0	0
-chrIII	168210	169490	peak_105	0	.	168250	168510	0	1	260	40	0	0	0
-chrIII	175560	177690	peak_106	0	.	176570	176980	0	2	110,260	1010,1160	0	0	0
-chrIII	177730	193340	peak_107	0	.	178140	193240	0	3	840,190,130	410,1530,15380	0	0	0
-chrIII	194460	208940	peak_108	0	.	197190	208600	0	2	290,210	2730,13930	0	0	0
-chrIII	210500	211540	peak_109	0	.	210990	211220	0	1	230	490	0	0	0
-chrIII	224130	224830	peak_110	0	.	224310	224540	0	1	230	180	0	0	0
-chrIII	226790	227790	peak_111	0	.	226990	227180	0	1	190	200	0	0	0
-chrIII	229550	237690	peak_112	0	.	236480	237180	0	2	150,400	6930,7230	0	0	0
-chrIII	239050	241610	peak_113	0	.	240370	240710	0	1	340	1320	0	0	0
-chrIII	253850	261680	peak_114	0	.	258710	259090	0	1	380	4860	0	0	0
-chrIII	263020	264520	peak_115	0	.	263100	263670	0	2	110,340	80,310	0	0	0
-chrIII	264590	280800	peak_116	0	.	264870	280060	0	5	280,350,340,180,180	280,1690,2400,9570,15290	0	0	0
-chrIII	289430	294780	peak_117	0	.	289610	289720	0	1	110	180	0	0	0
-chrIII	295250	299380	peak_118	0	.	295410	299250	0	2	340,310	160,3690	0	0	0
-chrIII	299420	309300	peak_119	0	.	305720	306040	0	1	320	6300	0	0	0
-chrIV	1750	19330	peak_120	0	.	5510	5790	0	1	280	3760	0	0	0
-chrIV	19380	20790	peak_121	0	.	20010	20430	0	1	420	630	0	0	0
-chrIV	21320	32480	peak_122	0	.	31960	32120	0	1	160	10640	0	0	0
-chrIV	43150	48680	peak_123	0	.	43260	46450	0	2	600,120	110,3180	0	0	0
-chrIV	48730	49760	peak_124	0	.	49320	49610	0	1	290	590	0	0	0
-chrIV	49840	62130	peak_125	0	.	56700	61680	0	4	190,170,240,690	6860,7600,9600,11150	0	0	0
-chrIV	63110	73420	peak_126	0	.	68680	68900	0	1	220	5570	0	0	0
-chrIV	73520	79730	peak_127	0	.	74050	77180	0	2	480,420	530,3240	0	0	0
-chrIV	83900	86930	peak_128	0	.	86070	86240	0	1	170	2170	0	0	0
-chrIV	87880	104740	peak_129	0	.	88070	98550	0	2	280,370	190,10300	0	0	0
-chrIV	107550	125100	peak_130	0	.	116020	124760	0	4	170,160,450,250	8470,9580,9790,16960	0	0	0
-chrIV	125130	125950	peak_131	0	.	125210	125580	0	1	370	80	0	0	0
-chrIV	127090	131980	peak_132	0	.	130370	130860	0	1	490	3280	0	0	0
-chrIV	135680	145890	peak_133	0	.	145390	145680	0	1	290	9710	0	0	0
-chrIV	150300	158820	peak_134	0	.	156000	156250	0	1	250	5700	0	0	0
-chrIV	158900	172240	peak_135	0	.	159360	167860	0	3	130,190,670	460,730,8290	0	0	0
-chrIV	176250	198410	peak_136	0	.	176290	190840	0	2	270,150	40,14440	0	0	0
-chrIV	206140	211470	peak_137	0	.	211060	211320	0	1	260	4920	0	0	0
-chrIV	212180	215770	peak_138	0	.	212700	213090	0	1	390	520	0	0	0
-chrIV	216750	218840	peak_139	0	.	217170	217650	0	1	480	420	0	0	0
-chrIV	218910	219890	peak_140	0	.	219390	219520	0	1	130	480	0	0	0
-chrIV	219940	222430	peak_141	0	.	221280	222250	0	2	140,450	1340,1860	0	0	0
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+chrXIV	559180	560180	peak_234	0	.	559440	560020	0	1	580	260	0	0	0
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+chrXIV	619750	620440	peak_237	0	.	619950	620430	0	1	480	200	0	0	0
+chrXIV	707270	708760	peak_238	0	.	707610	708750	0	1	1140	340	0	0	0
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+chrXV	31700	32650	peak_240	0	.	31820	32370	0	1	550	120	0	0	0
+chrXV	79760	80230	peak_241	0	.	79800	80190	0	1	390	40	0	0	0
+chrXV	108920	110220	peak_242	0	.	109030	109930	0	1	900	110	0	0	0
+chrXV	117950	118750	peak_243	0	.	118100	118330	0	1	230	150	0	0	0
+chrXV	159560	161950	peak_244	0	.	159610	161860	0	2	1680,510	50,1790	0	0	0
+chrXV	201880	202500	peak_245	0	.	201950	202410	0	1	460	70	0	0	0
+chrXV	216180	216890	peak_246	0	.	216390	216830	0	1	440	210	0	0	0
+chrXV	233740	234730	peak_247	0	.	234110	234670	0	1	560	370	0	0	0
+chrXV	253560	254210	peak_248	0	.	253630	254100	0	1	470	70	0	0	0
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+chrXV	304090	305180	peak_250	0	.	304230	305020	0	1	790	140	0	0	0
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+chrXV	595110	595820	peak_255	0	.	595310	595730	0	1	420	200	0	0	0
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+chrXV	911800	912760	peak_260	0	.	911950	912360	0	1	410	150	0	0	0
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