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Fatal error: atac.bds, line 1200, pos 2. Task/s failed....during peakcalling #134

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grrompala opened this issue Jul 16, 2018 · 1 comment

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@grrompala
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pipe_out_unfilt.txt

Hi, I am encountering the errors described in the log at the macs2 step of the pipeline. I am starting with BAM files-- I have tried both filtered and unfiltered. Moreover, I tried boosting the mem_macs2 and I still have the issue.

Let me know if I can provide any more insight and thanks for the help!!

-Greg

@leepc12
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leepc12 commented Jul 16, 2018

You have irregular chromosomes (chr20_GL383577v2_alt), which is not covered our default chromosome sizes file, in your BAM file. Please use your own chromosome sizes file (define it with -chrsz) or start from FASTQs.

Chromosome chr20_GL383577v2_alt isn't in /sc/orga/work/rompag01/genome_data/hg38/hg38.chrom.sizes line 1 of stdin: chr20_GL383577v2_alt:0-101601

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