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strain3C

strain3C is a workflow to resolve strain genomes from metagenomic data. strain3C requires as input:

  1. A polished long read assembly in GFA format
  2. A name-sorted bamfile containing Hi-C read pairs mapped against the assembly contigs
  3. A tabix-indexed set of variant calls in VCF format, made from the Hi-C reads mapped against the long read assembly

Usage

./strain3C --gfa polished.gfa --bam hic.namesorted.bam --vcf hic.vcf.gz -resume

WARNING

strain3C is currently a work in progress and UNLIKELY TO WORK CORRECTLY. Please contact the authors if you are interested in using it or contributing to its development.