Results with custom MSA are different and misfolded compared to the result from --use_msa_server

[kartik-rallapalli]

I am trying to fold a protein+RNA structure and while the predict fold is correct in case of the server generated MSAs (screenshot with green ribbons)(

), when I try to predict the same complex with a custom MSA the RNA fold seems distorted(orange in the screenshot

). Intriguingly, the custom MSA used in this case is the output generated from the --use-msa-server. Has anyone encountered/resolved such a issue yet? Thank you!

[wehs7661]

Sorry I am not sure how to resolve this issue but I wonder what MSA file you used from the case where --use-msa-server. Did you use bfd*.a3m or uniref.a3m in the output msa folder? Thanks a lot!

[kartik-rallapalli]

Thanks for taking a look at this issue. I used the uniref.a3m file! I believe that there is something in the way last line of any custom msa input file is processed (line 4459 in the screenshot below).

Along these lines, are the custom MSA required to have the uniref fasta IDs? Do these MSA homologs help decide the pdb templates like in Alphafold?

Thanks again for creating this repo and sharing the amazing work!