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Analysis of a TMA #291

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AlbertoFabbri93 opened this issue Jan 10, 2025 · 0 comments
Open

Analysis of a TMA #291

AlbertoFabbri93 opened this issue Jan 10, 2025 · 0 comments

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@AlbertoFabbri93
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AlbertoFabbri93 commented Jan 10, 2025 •
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Hi, it is my first time using CellChat and I was wondering what is the correct approach to analyze a TMA. I have the following slide as a single image in a Seurat object and I want to find the shared ligand-receptor interactions:

TMA_LU001FFP03_primary_diagnosis_highlighted

As of right now, I am analyzing the entire slide together. The problem is that I get a median cell to cell distance that is very high and I was wondering if this is going to affect the analysis. Should I analyze the entire slide together or should I split it into the individual stamps?
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