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Minimize bead carry-over in TruSeq PCR-free #30

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jgruselius opened this issue Sep 27, 2017 · 0 comments
Open

Minimize bead carry-over in TruSeq PCR-free #30

jgruselius opened this issue Sep 27, 2017 · 0 comments
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@jgruselius
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In recent preps there have been beads left in several wells after the SPRI purifications. Try:

  • Increase elution volume but don't transfer all of it
  • Increase magnet time in elution
@jgruselius jgruselius self-assigned this Sep 27, 2017
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