From a674e2e0d9354c3084bc34c19832a4d0e02342bc Mon Sep 17 00:00:00 2001 From: Alexandr Chernov Date: Fri, 18 Nov 2022 15:50:47 +0100 Subject: [PATCH 001/116] Fixes a URL query parsing bug for Vcf to BAM mapping --- .../java/org/broad/igv/variant/VariantTrack.java | 13 +++++++++++-- 1 file changed, 11 insertions(+), 2 deletions(-) diff --git a/src/main/java/org/broad/igv/variant/VariantTrack.java b/src/main/java/org/broad/igv/variant/VariantTrack.java index d321097cff..8d7bacc484 100644 --- a/src/main/java/org/broad/igv/variant/VariantTrack.java +++ b/src/main/java/org/broad/igv/variant/VariantTrack.java @@ -236,8 +236,17 @@ public VariantTrack(ResourceLocator locator, FeatureSource source, List boolean bypassFileAutoDiscovery = prefMgr.getAsBoolean(BYPASS_FILE_AUTO_DISCOVERY); if (vcfToBamMapping == null && path != null && !bypassFileAutoDiscovery) { - if (ParsingUtils.fileExists(path + ".mapping")) { - vcfToBamMapping = path + ".mapping"; + String mappingFile = ""; + int queryStart = path.indexOf("?"); + if (queryStart > -1) + { + String query = path.substring(queryStart); + path = path.substring(0,queryStart); + mappingFile = path + ".mapping" + query; + } + + if (ParsingUtils.fileExists(mappingFile)) { + vcfToBamMapping = mappingFile; } } From df7d4ba85d833adb7d840f42b86210c4d381d56c Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 24 Nov 2022 22:48:14 -0800 Subject: [PATCH 002/116] . --- .../org/broad/igv/feature/genome/Genome.java | 2 +- .../feature/genome/load/JsonGenomeLoader.java | 12 ++++-- .../broad/igv/prefs/PreferencesManager.java | 10 +++-- .../java/org/broad/igv/track/BlatTrack.java | 3 ++ .../org/broad/igv/util/blat/BlatClient.java | 40 +++++++++++++------ 5 files changed, 45 insertions(+), 22 deletions(-) diff --git a/src/main/java/org/broad/igv/feature/genome/Genome.java b/src/main/java/org/broad/igv/feature/genome/Genome.java index cbf308e507..6d96bd4b62 100644 --- a/src/main/java/org/broad/igv/feature/genome/Genome.java +++ b/src/main/java/org/broad/igv/feature/genome/Genome.java @@ -383,7 +383,7 @@ public String getId() { } public String getBlatDB() { - return blatDB != null ? blatDB : id; + return blatDB; } public void setBlatDB(String blatDB) { diff --git a/src/main/java/org/broad/igv/feature/genome/load/JsonGenomeLoader.java b/src/main/java/org/broad/igv/feature/genome/load/JsonGenomeLoader.java index 4bcd63d53d..b8ea20e1f4 100644 --- a/src/main/java/org/broad/igv/feature/genome/load/JsonGenomeLoader.java +++ b/src/main/java/org/broad/igv/feature/genome/load/JsonGenomeLoader.java @@ -171,14 +171,18 @@ public Genome loadGenome() throws IOException { } } - JsonElement ucscIDElement = json.get("ucscID"); - if (ucscIDElement != null) { - newGenome.setUcscID(ucscIDElement.getAsString()); - } JsonElement blatDB = json.get("blatDB"); if (blatDB != null) { newGenome.setUcscID(blatDB.getAsString()); } + JsonElement ucscIDElement = json.get("ucscID"); + if (ucscIDElement != null) { + newGenome.setUcscID(ucscIDElement.getAsString()); + if(blatDB == null) { + newGenome.setBlatDB(ucscIDElement.getAsString()); + } + } + JsonElement aliasURL = json.get("aliasURL"); if (aliasURL != null) { String aliasPath = FileUtils.getAbsolutePath(aliasURL.getAsString(), genomePath); diff --git a/src/main/java/org/broad/igv/prefs/PreferencesManager.java b/src/main/java/org/broad/igv/prefs/PreferencesManager.java index a77dc88fbc..bce0d08f7d 100644 --- a/src/main/java/org/broad/igv/prefs/PreferencesManager.java +++ b/src/main/java/org/broad/igv/prefs/PreferencesManager.java @@ -24,7 +24,7 @@ public class PreferencesManager implements IGVEventObserver { private static List preferenceGroupList; private static Logger log = LogManager.getLogger(PreferencesManager.class); private static Map preferencesMap = Collections.synchronizedMap(new HashMap<>()); - private static IGVPreferences genericDefaults; + private static Map genericDefaults; private static String prefFile; // User preferences file @@ -79,15 +79,14 @@ private static void init() { } } - genericDefaults = new IGVPreferences(defaultPreferences.get(NULL_CATEGORY), null, null); - Map defaults = defaultPreferences.get(NULL_CATEGORY); + genericDefaults = defaultPreferences.get(NULL_CATEGORY); Map rnaDefaults = defaultPreferences.get(RNA); Map thirdGenDefaults = defaultPreferences.get(THIRD_GEN); Map> userPrefs = loadUserPreferences(); - final IGVPreferences nullPrefs = new IGVPreferences(userPrefs.get(NULL_CATEGORY), defaults, null); + final IGVPreferences nullPrefs = new IGVPreferences(userPrefs.get(NULL_CATEGORY), genericDefaults, null); extractMutationColors(nullPrefs); preferencesMap.put(NULL_CATEGORY, nullPrefs); preferencesMap.put(RNA, new IGVPreferences(userPrefs.get(RNA), rnaDefaults, nullPrefs)); @@ -116,6 +115,9 @@ private static void extractMutationColors(IGVPreferences prefs) { } } + public static String getDefault(String key) { + return genericDefaults.get(key); + } public static IGVPreferences getPreferences() { return getPreferences(NULL_CATEGORY); } diff --git a/src/main/java/org/broad/igv/track/BlatTrack.java b/src/main/java/org/broad/igv/track/BlatTrack.java index b9cc6760ee..18de6d1882 100644 --- a/src/main/java/org/broad/igv/track/BlatTrack.java +++ b/src/main/java/org/broad/igv/track/BlatTrack.java @@ -125,6 +125,9 @@ public void unmarshalXML(Element element, Integer version) { String db = element.getAttribute("db"); if (db == null || db.length() == 0) { db = GenomeManager.getInstance().getCurrentGenome().getBlatDB(); + if(db == null) { + db = GenomeManager.getInstance().getCurrentGenome().getId(); + } } try { this.features = BlatClient.blat(db, sequence); diff --git a/src/main/java/org/broad/igv/util/blat/BlatClient.java b/src/main/java/org/broad/igv/util/blat/BlatClient.java index 4c3ffb7942..6fac19bd40 100644 --- a/src/main/java/org/broad/igv/util/blat/BlatClient.java +++ b/src/main/java/org/broad/igv/util/blat/BlatClient.java @@ -43,9 +43,7 @@ import java.io.IOException; import java.net.URL; import java.net.URLEncoder; -import java.util.ArrayList; -import java.util.Iterator; -import java.util.List; +import java.util.*; /** * Port of perl script blatPlot.pl http://genomewiki.cse.ucsc.edu/index.php/Blat_Scripts @@ -63,10 +61,12 @@ public class BlatClient { public static List blat(String db, String userSeq) throws IOException { String serverType = PreferencesManager.getPreferences().get(Constants.BLAT_SERVER_TYPE, ""); - String urlpref = PreferencesManager.getPreferences().get( - Constants.BLAT_URL, - "https://genome.ucsc.edu/cgi-bin/hgBlat?userSeq=$SEQUENCE&type=DNA&db=$DB&output=json") - .trim(); + String urlpref = PreferencesManager.getPreferences().get(Constants.BLAT_URL); + if (urlpref == null || urlpref.trim().length() == 0) { + MessageUtils.showMessage("BLAT url is not configured"); + return Collections.EMPTY_LIST; + } + urlpref = urlpref.trim(); if (serverType.equalsIgnoreCase("web_blat") || !urlpref.contains("$SEQUENCE")) { return LegacyBlatClient.blat(userSeq); @@ -140,8 +140,16 @@ public static List blat(String db, String userSeq) throws IOException public static void doBlatQuery(final String userSeq, final String trackLabel) { try { - Genome genome = IGV.hasInstance() ? GenomeManager.getInstance().getCurrentGenome() : null; - String db = genome == null ? "hg19" : genome.getBlatDB(); + Genome genome = GenomeManager.getInstance().getCurrentGenome(); + String db = genome.getBlatDB(); + if (db == null) { + // If this is a known UCSC genome, or a custom blat server is configured, use the genome ID + if (knownUCSCGenomes.contains(genome.getId()) || + !PreferencesManager.getPreferences().get(Constants.BLAT_URL).equals(PreferencesManager.getDefault(Constants.BLAT_URL))) { + db = genome.getId(); + } + } + List features = blat(db, userSeq); if (features.isEmpty()) { MessageUtils.showMessage("No features found"); @@ -177,22 +185,28 @@ public static void doBlatQueryFromRegion(final String chr, final int start, fina doBlatQuery(userSeq, "BLAT"); } - private static String htmlToString (String str) { + private static String htmlToString(String str) { // Remove script tags int idx1; int count = 0; - while((idx1 = str.indexOf("= 0 && count < 10) { + while ((idx1 = str.indexOf("= 0 && count < 10) { int idx2 = str.indexOf("", idx1); - if(idx1 > 0 && idx2 > idx1) { + if (idx1 > 0 && idx2 > idx1) { str = str.substring(0, idx1) + str.substring(idx2 + 9); } count++; } - str = str.replaceAll("\\<.*?>","").replace('\n', ' '); + str = str.replaceAll("\\<.*?>", "").replace('\n', ' '); return str; } + static Set knownUCSCGenomes = new HashSet<>(Arrays.asList("hg18", "hg19", "hg38", "ce10", "ce11", "galGal4", + "galGal5", "galGal6", "panTro3", "panTro4", "panTro5", "panTro6", "felCat6", "bosTau7", "bosTau8", + "bosTau9", "dm3", "dm6", "canFam3", "canFam5", "mm8", "mm9", "mm10", "mm39", "rn5", "rn6", "rn7", + "rheMac3", "rheMac8", "rheMac10", "sacCer3", "susScr3", "xenTro9", "danRer10", "danRer11", + "gorGor4", "gorGor6", "panPan2", "susScr11", "strPur2")); + } From d1b65b43c3f534fba4d8531744a82f9c82aa150c Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Fri, 25 Nov 2022 21:39:03 -0800 Subject: [PATCH 003/116] Add new html paramater option "merge=ask". Fixes #1261 --- .../org/broad/igv/batch/CommandListener.java | 8 ++++++-- test/web/StaticLinkExamples.html | 20 +++++++++++++++++++ 2 files changed, 26 insertions(+), 2 deletions(-) diff --git a/src/main/java/org/broad/igv/batch/CommandListener.java b/src/main/java/org/broad/igv/batch/CommandListener.java index 2672d46bc0..57ba8b7c4c 100755 --- a/src/main/java/org/broad/igv/batch/CommandListener.java +++ b/src/main/java/org/broad/igv/batch/CommandListener.java @@ -35,6 +35,7 @@ import org.broad.igv.prefs.Constants; import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.ui.IGV; +import org.broad.igv.ui.util.MessageUtils; import org.broad.igv.ui.util.UIUtilities; import org.broad.igv.util.HttpUtils; import org.broad.igv.util.StringUtils; @@ -389,8 +390,11 @@ private String processGet(String command, Map params, CommandExe // Default for merge is "false" for session files, "true" otherwise boolean merge; if (mergeValue != null) { - // Explicit setting - merge = mergeValue.equalsIgnoreCase("true"); + if("ask".equals(mergeValue)) { + merge = !MessageUtils.confirm("Unload current session before loading new tracks?"); + } else { + merge = mergeValue.equalsIgnoreCase("true"); + } } else if (file.endsWith(".xml") || file.endsWith(".php") || file.endsWith(".php3")) { // Session file merge = false; diff --git a/test/web/StaticLinkExamples.html b/test/web/StaticLinkExamples.html index 67ddecafda..b9bff4e26b 100644 --- a/test/web/StaticLinkExamples.html +++ b/test/web/StaticLinkExamples.html @@ -7,6 +7,18 @@

IGV desktop links

+

+ + Load merge=false +

+

+ + Load merge=true +

+

+ + Load merge=ask +

Load with name @@ -23,6 +35,10 @@

IGV desktop links

Load with name into new session

+

+ + Load with name into current session +

Load with index @@ -43,6 +59,10 @@

IGV desktop links

Load session

+

+ + Load session +

Load session with initial locus (chr22) From 78bb4cced2932df15a41ad8c63bcbac096401bec Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Sat, 26 Nov 2022 21:02:18 -0800 Subject: [PATCH 004/116] Use static URLDecoder.decode method for compatibility with Java 17. Fixes #1259 --- src/main/java/org/broad/igv/oauth/OAuthProvider.java | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/src/main/java/org/broad/igv/oauth/OAuthProvider.java b/src/main/java/org/broad/igv/oauth/OAuthProvider.java index 655815ff4d..be12784ed9 100644 --- a/src/main/java/org/broad/igv/oauth/OAuthProvider.java +++ b/src/main/java/org/broad/igv/oauth/OAuthProvider.java @@ -201,7 +201,7 @@ public void setAuthorizationCode(String authorizationCode) throws IOException { if (clientSecret != null) { params.put("client_secret", clientSecret); } - params.put("redirect_uri", new URLDecoder().decode(redirectURI, "utf-8")); + params.put("redirect_uri", URLDecoder.decode(redirectURI, "utf-8")); params.put("grant_type", "authorization_code"); // set the resource if it necessary for the auth provider dwm08 From 13469778dbb2d227865d4c58486b179b6c10985c Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Sat, 26 Nov 2022 21:44:33 -0800 Subject: [PATCH 005/116] Allow quoted string to specify genome path with spaces in batch script. Fixes #1258 --- .../org/broad/igv/batch/CommandExecutor.java | 55 ++++++++----------- test/batch/path_with_spaces.txt | 4 ++ 2 files changed, 28 insertions(+), 31 deletions(-) diff --git a/src/main/java/org/broad/igv/batch/CommandExecutor.java b/src/main/java/org/broad/igv/batch/CommandExecutor.java index 578ffdfc4a..032865a8b8 100755 --- a/src/main/java/org/broad/igv/batch/CommandExecutor.java +++ b/src/main/java/org/broad/igv/batch/CommandExecutor.java @@ -104,7 +104,7 @@ public String execute(String commandLine) { log.debug("Executing: " + commandLine); try { if (args.size() == 0) { - return "Empty command string"; + return result; } String cmd = args.get(0).toLowerCase(); @@ -623,25 +623,14 @@ private String genome(String param1) { return result; } - String genomePath = genomeID; - if (!ParsingUtils.fileExists(genomePath)) { - String workingDirectory = System.getProperty("user.dir", ""); - genomePath = FileUtils.getAbsolutePath(genomeID, workingDirectory); - } - if (ParsingUtils.fileExists(genomePath)) { - try { - GenomeManager.getInstance().loadGenome(genomePath, null); - } catch (IOException e) { - result = "ERROR: Could not load genome: " + genomeID; - MessageUtils.showMessage(result); - } - - } else { - result = "ERROR: Could not locate genome: " + genomeID; + String genomePath = resolveFileReference(genomeID); + try { + GenomeManager.getInstance().loadGenome(genomePath, null); + } catch (IOException e) { + result = "ERROR: Could not load genome: " + genomeID; MessageUtils.showMessage(result); } - return result; } @@ -756,18 +745,8 @@ String loadFiles(final String fileString, // Loop through files for (int fi = 0; fi < files.size(); fi++) { - String f = files.get(fi); - if (!FileUtils.isRemote(f)) { - File maybeFile = getFile(f); - if (maybeFile.exists()) { - f = maybeFile.getAbsolutePath(); - } else { - maybeFile = new File(this.scriptDir, StringUtils.stripQuotes(f)); - if (maybeFile.exists()) { - f = maybeFile.getAbsolutePath(); - } - } - } + + String f = resolveFileReference(files.get(fi)); if (isDataURL && formats == null) { return "Error: format must be specified for dataURLs"; @@ -840,6 +819,19 @@ String loadFiles(final String fileString, return CommandListener.OK; } + private String resolveFileReference(String f) { + + if (FileUtils.isRemote(f)) { + return f; + } else { + File maybeFile = getFile(f); + if (maybeFile.exists()) { + f = maybeFile.getAbsolutePath(); + } + return f; + } + } + static List breakFileString(String fileString) { if (fileString == null) { @@ -956,6 +948,7 @@ String setSnapshotDirectory(String param1) { * @return */ private File getFile(String path) { + // Strip trailing & leading quotes path = StringUtils.stripQuotes(path); @@ -1050,11 +1043,11 @@ private String sort(String sortArg, String param2, String param3, String param4) reverseString = param4; } else { locusString = param2; - reverseString = param3; + reverseString = param3; } // Special case, "reverse" is a resered word for inverting sorting. Locus is optional - if(reverseString == null && "reverse".equalsIgnoreCase(locusString)) { + if (reverseString == null && "reverse".equalsIgnoreCase(locusString)) { reverseString = locusString; locusString = null; } diff --git a/test/batch/path_with_spaces.txt b/test/batch/path_with_spaces.txt index 9fb329db7b..03434cf820 100644 --- a/test/batch/path_with_spaces.txt +++ b/test/batch/path_with_spaces.txt @@ -22,3 +22,7 @@ goto chr1:16270500-16270600 load "../data/folder with spaces/test+wp.wig" snapshot "test+wp.png" +# Test genome in quoted string +genome "../data/genomes/hg18.unittest.genome" + + From da44106a15a7d549cce57bafc822ec03b4ff5d8b Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Sun, 27 Nov 2022 20:21:54 -0800 Subject: [PATCH 006/116] Simplify "isGoogle" tests --- src/main/java/org/broad/igv/track/TrackLoader.java | 2 +- .../org/broad/igv/ui/action/LoadFromURLMenuAction.java | 4 ++-- src/main/java/org/broad/igv/util/GoogleUtils.java | 5 +---- src/main/java/org/broad/igv/util/HttpUtils.java | 7 +++++-- 4 files changed, 9 insertions(+), 9 deletions(-) diff --git a/src/main/java/org/broad/igv/track/TrackLoader.java b/src/main/java/org/broad/igv/track/TrackLoader.java index 7896ecb6a6..a4ca8716f3 100644 --- a/src/main/java/org/broad/igv/track/TrackLoader.java +++ b/src/main/java/org/broad/igv/track/TrackLoader.java @@ -938,7 +938,7 @@ private void loadAlignmentsTrack(ResourceLocator locator, List newTracks, String path = locator.getPath(); boolean bypassFileAutoDiscovery = PreferencesManager.getPreferences().getAsBoolean(BYPASS_FILE_AUTO_DISCOVERY) || - GoogleUtils.isGoogleCloud(locator.getPath()) || + GoogleUtils.isGoogleURL(locator.getPath()) || path.contains("dropbox.com") || path.contains("dataformat=.bam") || path.contains("/query.cgi?"); diff --git a/src/main/java/org/broad/igv/ui/action/LoadFromURLMenuAction.java b/src/main/java/org/broad/igv/ui/action/LoadFromURLMenuAction.java index 8c3d2c9152..e46c76bd3b 100644 --- a/src/main/java/org/broad/igv/ui/action/LoadFromURLMenuAction.java +++ b/src/main/java/org/broad/igv/ui/action/LoadFromURLMenuAction.java @@ -109,7 +109,7 @@ public void actionPerformed(ActionEvent e) { if (dlg.getIndexURL() != null) { String indexUrl = dlg.getIndexURL().trim(); - if (GoogleUtils.isGoogleCloud(indexUrl) || GoogleUtils.isGoogleDrive(indexUrl)) { + if (GoogleUtils.isGoogleURL(indexUrl)) { enableGoogleMenu(); } @@ -151,7 +151,7 @@ public void actionPerformed(ActionEvent e) { private String mapURL(String url) { url = url.trim(); - if (GoogleUtils.isGoogleCloud(url) || GoogleUtils.isGoogleDrive(url)) { + if (GoogleUtils.isGoogleURL(url)) { enableGoogleMenu(); } diff --git a/src/main/java/org/broad/igv/util/GoogleUtils.java b/src/main/java/org/broad/igv/util/GoogleUtils.java index 297a31dd08..079f724c3c 100644 --- a/src/main/java/org/broad/igv/util/GoogleUtils.java +++ b/src/main/java/org/broad/igv/util/GoogleUtils.java @@ -29,12 +29,9 @@ public class GoogleUtils { public static final String GOOGLE_DRIVE_HOST = "drive.google.com"; public static boolean isGoogleURL(String url) { - return url != null && (isGoogleCloud(url) || isGoogleDrive(url) || isGoogleStorageURL(url)); + return url != null && (isGoogleDrive(url) || isGoogleStorageURL(url)); } - public static boolean isGoogleCloud(String url) { - return url != null && (url.startsWith("gs://") || url.contains(GOOGLE_API_HOST)); - } public static boolean isGoogleDrive(String url) { return url != null && (url.contains(GOOGLE_DRIVE_HOST) || url.contains("www.googleapis.com/drive")); diff --git a/src/main/java/org/broad/igv/util/HttpUtils.java b/src/main/java/org/broad/igv/util/HttpUtils.java index 5acfcd4946..47fbd7ece0 100644 --- a/src/main/java/org/broad/igv/util/HttpUtils.java +++ b/src/main/java/org/broad/igv/util/HttpUtils.java @@ -203,7 +203,7 @@ public static String mapURL(String urlString) throws MalformedURLException { urlString = GoogleUtils.translateGoogleCloudURL(urlString); } - if (GoogleUtils.isGoogleCloud(urlString)) { + if (GoogleUtils.isGoogleURL(urlString)) { if (urlString.indexOf("alt=media") < 0) { urlString = URLUtils.addParameter(urlString, "alt=media"); } @@ -679,12 +679,14 @@ private HttpURLConnection openConnection( // If the URL is protected via an oAuth provider fetch token, and optionally map url with find/replace string. OAuthProvider oauthProvider = OAuthUtils.getInstance().getProviderForURL(url); + if (oauthProvider != null) { //Google is skipped here as we don't yet know if the url is protected or not. Login is invoked after 401 error if(!oauthProvider.isGoogle()) { oauthProvider.checkLogin(); } token = oauthProvider.getAccessToken(); + if (oauthProvider.findString != null) { // A hack, supported for backward compatibility but not reccomended url = HttpUtils.createURL(url.toExternalForm().replaceFirst(oauthProvider.findString, oauthProvider.replaceString)); @@ -720,6 +722,7 @@ private HttpURLConnection openConnection( GoogleUtils.getProjectID() != null && GoogleUtils.getProjectID().length() > 0 && !hasQueryParameter(url, "userProject")) { + url = addQueryParameter(url, "userProject", GoogleUtils.getProjectID()); } @@ -860,7 +863,7 @@ else if (code >= 400) { message = conn.getResponseMessage(); String details = readErrorStream(conn); - if (url.getHost().equals("www.googleapis.com") && details.contains("requester pays bucket")) { + if (GoogleUtils.isGoogleURL(url.toExternalForm()) && details.contains("requester pays bucket")) { MessageUtils.showMessage("" + details + "
Use Google menu to set project."); } From ffee518bff024d8568e257d888589b0db903efe7 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Mon, 28 Nov 2022 10:08:00 -0800 Subject: [PATCH 007/116] Handle "error" response to oAuth authorization callbacks -- e.g. user denies authorization --- .../java/org/broad/igv/batch/CommandListener.java | 13 +++++++++---- 1 file changed, 9 insertions(+), 4 deletions(-) diff --git a/src/main/java/org/broad/igv/batch/CommandListener.java b/src/main/java/org/broad/igv/batch/CommandListener.java index 57ba8b7c4c..df99b658a2 100755 --- a/src/main/java/org/broad/igv/batch/CommandListener.java +++ b/src/main/java/org/broad/igv/batch/CommandListener.java @@ -225,15 +225,20 @@ private void processClientSession(CommandExecutor cmdExe) throws IOException { if (command.equals("/oauthCallback")) { OAuthProvider provider = OAuthUtils.getInstance().getProviderForState(params.get("state")); - - if (params.containsKey("code")) { + if (params.containsKey("error")) { + sendTextResponse(out, "Error authorizing IGV: " + params.get("error")); + } else if (params.containsKey("code")) { provider.setAuthorizationCode(params.get("code")); + sendTextResponse(out, "Authorization successful. You may close this tab."); } else if (params.containsKey("token")) { // Very doubtful this is ever called -- its not a normal OAuth flow log.info("Oauth token received"); provider.setAccessToken(params.get("token")); + sendTextResponse(out, "Authorization successful. You may close this tab."); + } else { + sendTextResponse(out, "Unsuccessful authorization response: " + inputLine); } - sendTextResponse(out, "Authorization successful. You may close this tab."); + if (PreferencesManager.getPreferences().getAsBoolean(Constants.PORT_ENABLED) == false) { // Turn off port @@ -390,7 +395,7 @@ private String processGet(String command, Map params, CommandExe // Default for merge is "false" for session files, "true" otherwise boolean merge; if (mergeValue != null) { - if("ask".equals(mergeValue)) { + if ("ask".equals(mergeValue)) { merge = !MessageUtils.confirm("Unload current session before loading new tracks?"); } else { merge = mergeValue.equalsIgnoreCase("true"); From 0f6d6ec8f21701a8faf7d857fe9fa187abcf6e47 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Mon, 28 Nov 2022 22:07:21 -0800 Subject: [PATCH 008/116] Introduce user preference and file directives for "mut" format coordinate convention Revert default coordinate convention for "mut" format to UCSC style "0" base Fixes #1226 --- .../broad/igv/feature/tribble/MUTCodec.java | 43 +- .../java/org/broad/igv/prefs/Constants.java | 2 + .../org/broad/igv/prefs/preferences.tab | 2 + ...cga_20080711_phase1_filtered_nonsilent.mut | 455 ++++++++++++++++++ 4 files changed, 498 insertions(+), 4 deletions(-) create mode 100644 test/data/mut/tcga_20080711_phase1_filtered_nonsilent.mut diff --git a/src/main/java/org/broad/igv/feature/tribble/MUTCodec.java b/src/main/java/org/broad/igv/feature/tribble/MUTCodec.java index f43b87df99..27396107cb 100644 --- a/src/main/java/org/broad/igv/feature/tribble/MUTCodec.java +++ b/src/main/java/org/broad/igv/feature/tribble/MUTCodec.java @@ -34,6 +34,8 @@ import org.broad.igv.exceptions.DataLoadException; import org.broad.igv.feature.Mutation; import org.broad.igv.feature.genome.Genome; +import org.broad.igv.prefs.PreferencesManager; +import org.broad.igv.track.TrackProperties; import org.broad.igv.util.ParsingUtils; import org.broad.igv.util.ResourceLocator; @@ -45,6 +47,8 @@ import java.util.LinkedHashMap; import java.util.Map; +import static org.broad.igv.prefs.Constants.MUT_COORDS; + /** * Codec for .mut and .maf mutation files * @@ -70,6 +74,7 @@ public class MUTCodec extends AsciiFeatureCodec { private int tumorAllele1Column = -1; private int tumorAllele2Column = -1; private int errorCount = 0; + private TrackProperties.BaseCoord coords = TrackProperties.BaseCoord.ZERO; public MUTCodec(String path, Genome genome) { @@ -92,10 +97,10 @@ public MUTCodec(String path, Genome genome) { } public Void readActualHeader(LineIterator reader) { - String nextLine = null; + TrackProperties.BaseCoord coordsValue = null; while (reader.hasNext()) { - nextLine = reader.peek(); + String nextLine = reader.peek(); if (nextLine.startsWith("#")) { if (nextLine.startsWith("#samples")) { String[] tokens = Globals.whitespacePattern.split(nextLine, 2); @@ -107,11 +112,33 @@ public Void readActualHeader(LineIterator reader) { samples[i] = samples[i].trim(); } } + } else if (nextLine.startsWith("#coords")) { + String[] tokens = Globals.equalPattern.split(nextLine); + if(tokens.length > 1) { + String value = tokens[1].trim(); + if (value.equals("0")) { + coordsValue = TrackProperties.BaseCoord.ZERO; + } else if (value.equals("1")) { + coordsValue = TrackProperties.BaseCoord.ONE; + } else { + log.error("Unknown #coords value: " + value + " Legal values are 0 and 1"); + } + } else { + log.error("#coords directive must have value, e.g. #coords=1"); + } } reader.next(); continue; } + // If coords are explicitly set use setting, otherwise check preferences + if(coordsValue != null) { + this.coords = coordsValue; + } else { + this.coords = "1".equals(PreferencesManager.getPreferences().get(MUT_COORDS, "0")) ? + TrackProperties.BaseCoord.ONE : TrackProperties.BaseCoord.ZERO; + } + String[] tokens = Globals.tabPattern.split(nextLine); if (tokens.length >= 5) { reader.next(); @@ -153,7 +180,7 @@ public Mutation decode(String line) { int start; try { - start = Integer.parseInt(tokens[startColumn].trim()) - 1; + start = Integer.parseInt(tokens[startColumn].trim()); } catch (NumberFormatException e) { errorCount++; if (errorCount > 100) { @@ -177,6 +204,14 @@ public Mutation decode(String line) { } } + + // MAF files use the 1-based inclusive convention for coordinates. The convention is not + // specified for MUT files, and it appears both conventions have been used. We can detect + // the convention used for single base mutations by testing start == end. + if (isMAF || (start == end) || coords == TrackProperties.BaseCoord.ONE) { + start--; + } + String sampleId = "Unknown"; if(sampleColumn >= 0) { sampleId = tokens[sampleColumn].trim(); @@ -422,6 +457,6 @@ public static boolean isMafLiteFile(String[] tokens) { @Override public boolean canDecode(String path) { - return isMutationAnnotationFile(new ResourceLocator(path)); + return isMutationAnnotationFile(new ResourceLocator(path)); } } diff --git a/src/main/java/org/broad/igv/prefs/Constants.java b/src/main/java/org/broad/igv/prefs/Constants.java index b86f85bbff..bdc8cad8cb 100644 --- a/src/main/java/org/broad/igv/prefs/Constants.java +++ b/src/main/java/org/broad/igv/prefs/Constants.java @@ -213,6 +213,8 @@ private Constants() { public static final String OVERLAY_MUTATIONS_WHOLE_GENOME = "OVERLAY_MUTATIONS_WHOLE_GENOME"; public static final String COLOR_MUTATIONS = "COVER_OVERLAY_KEY"; + public static final String MUT_COORDS = "MUT_COORDS"; + // GWAS track options public static final String GWAS_TRACK_HEIGHT = "GWAS_TRACK_HEIGHT"; public static final String GWAS_DESCRIPTION_CACHE_SIZE = "GWAS_DESCRIPTION_CACHE_SIZE"; diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index e21c57d36f..cc4b8df723 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -55,6 +55,8 @@ MUTATION_Non-coding_Transcript_COLOR Non-coding Transcript color 0,0,150 MUTATION_Other_AA_changing_COLOR Other AA Changing color 0,150,150 MUTATION_Other_likely_neutral_COLOR Other Likely Neutral color 225,180,225 MUTATION_UNKNOWN_COLOR_KEY Unknown color 0,180,225 +##Coordinate System +MUT_COORDS Mut file coordinate convention. 0 = zero based half-open, 1 = one based fully closed. select 0|1 0 #Charts CHART.DRAW_TOP_BORDER Draw top border boolean FALSE diff --git a/test/data/mut/tcga_20080711_phase1_filtered_nonsilent.mut b/test/data/mut/tcga_20080711_phase1_filtered_nonsilent.mut new file mode 100644 index 0000000000..efe297f99d --- /dev/null +++ b/test/data/mut/tcga_20080711_phase1_filtered_nonsilent.mut @@ -0,0 +1,455 @@ +#coords=1 +chr start end patient type gene phase center shared strand site supertype context class newbase status validation call evidence filtered use hypermutated +1 2072180 2072180 TCGA-06-0206 Missense PRKCZ 1 BCM No + PRKCZ|chr1:2072180-2072180 Missense cgggcGcggga G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +1 2106249 2106249 TCGA-02-0083 Missense PRKCZ 1 BCM No + PRKCZ|chr1:2106249-2106249 Missense agtcaGagttc G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 6112865 6112865 TCGA-06-0189 Missense CHD5 1 genome.wustl.edu No - CHD5|chr1:6112865-6112865 Missense acccgCacaat C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +1 11224264 11224264 TCGA-02-0083 Missense FRAP1 1 genome.wustl.edu No - FRAP1|chr1:11224264-11224264 Missense ctcgaGcaatg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 12175619 12175619 TCGA-06-0126 Missense TNFRSF1B 1 genome.wustl.edu No + TNFRSF1B|chr1:12175619-12175619 Missense tgtccAcacga A C Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +1 15717209 15717209 TCGA-02-0083 Missense CASP9 1 BCM No - CASP9|chr1:15717209-15717209 Missense ttctgGaggat G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 16347951 16347951 TCGA-02-0114 Missense EPHA2 1 BCM No - EPHA2|chr1:16347951-16347951 Missense tggtgGcgcca G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 23758222 23758222 TCGA-02-0114 Missense ID3 1 broad.mit.edu No - ID3|chr1:23758222-23758222 Missense atggcCcccac C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 27555547 27555547 TCGA-02-0028 Missense MAP3K6 1 broad.mit.edu No - MAP3K6|chr1:27555547-27555547 Missense aacggGagtac G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 114742087 114742087 TCGA-02-0010 Nonsense TRIM33 1 broad.mit.edu No - TRIM33|chr1:114742087-114742087 Nonsense atttgAaggta A T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 115060270 115060270 TCGA-02-0085 Missense NRAS 1 BCM No - NRAS|chr1:115060270-115060270 Missense agcagGtggtg G (other) A Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +1 149055490 149055490 TCGA-02-0083 Missense ARNT 1 genome.wustl.edu No - ARNT|chr1:149055490-149055490 Missense tagccCctcct C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 153299877 153299877 TCGA-02-0083 Missense ADAM15 1 BCM No + ADAM15|chr1:153299877-153299877 Missense atctgGtccct G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 165625522 165625522 TCGA-02-0043 Missense POU2F1 1 BCM No + POU2F1|chr1:165625522-165625522 Missense aaagcGaattg G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 195340175 195340175 TCGA-02-0010 Missense ASPM 1 broad.mit.edu No - ASPM|chr1:195340175-195340175 Missense agctgTtataa T C Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 195365053 195365053 TCGA-02-0010 Missense ASPM 1 broad.mit.edu No - ASPM|chr1:195365053-195365053 Missense ctttgTccaag T C Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 202010148 202010148 TCGA-02-0114 Missense LAX1 1 genome.wustl.edu No + LAX1|chr1:202010148-202010148 Missense gtggaAgccag A T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 202674772 202674772 TCGA-02-0043 Missense PIK3C2B 1 broad.mit.edu No - PIK3C2B|chr1:202674772-202674772 Missense aggtgGagcat G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 202774027 202774027 TCGA-06-0129 Missense MDM4 1 BCM, broad.mit.edu, genome.wustl.edu Yes + MDM4|chr1:202774027-202774027 Missense gcctaCctcag C (other) G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +1 202854501 202854501 TCGA-02-0043 Missense LRRN2 1 BCM No - LRRN2|chr1:202854501-202854501 Missense tccggGagatg G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 206025059 206025059 TCGA-02-0010 Missense CD46 1 BCM No + CD46|chr1:206025059-206025059 Missense ctgtgAttgtt A G Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 212237253 212237253 TCGA-02-0010 Missense PROX1 1 broad.mit.edu No + PROX1|chr1:212237253-212237253 Missense gctgcGccagc G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +1 212853776 212853776 TCGA-02-0055 Missense CENPF 1 genome.wustl.edu No + CENPF|chr1:212853776-212853776 Missense tcagaAaattc A G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +1 212888794 212888794 TCGA-02-0099 Splice_site CENPF 1 genome.wustl.edu No + CENPF|chr1:212888794-212888794 Other gcaaaGtaagt G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 5751538 5751538 TCGA-06-0173 Nonsense SOX11 1 broad.mit.edu No + SOX11|chr2:5751538-5751538 Nonsense acttcGagttc G (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +2 5751587 5751587 TCGA-02-0010 Missense SOX11 1 broad.mit.edu No + SOX11|chr2:5751587-5751587 Missense gggggActggc A G Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 15999768 15999768 TCGA-02-0028 Missense MYCN 1 broad.mit.edu No + MYCN|chr2:15999768-15999768 Missense gacccCcccgg C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 47484039 47484039 TCGA-02-0075 Missense MSH2 1 genome.wustl.edu No + MSH2|chr2:47484039-47484039 Missense tggggCcggca C (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +2 47557079 47557079 TCGA-02-0083 Missense MSH2 1 genome.wustl.edu No + MSH2|chr2:47557079-47557079 Missense aattgGgtgtt G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 47880076 47880076 TCGA-02-0114 Missense MSH6 1 BCM No + MSH6|chr2:47880076-47880076 Missense gagtgGaacag G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 47881696 47881696 TCGA-02-0010 Missense MSH6 1 BCM No + MSH6|chr2:47881696-47881696 Missense aagaaCggagg C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 47881792 47881792 TCGA-02-0083 Missense MSH6 1 BCM No + MSH6|chr2:47881792-47881792 Missense tcgcaGtgttg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 47886856 47886856 TCGA-02-0043 Missense MSH6 1 BCM No + MSH6|chr2:47886856-47886856 Missense aggtaCtgcaa C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 60541333 60541333 TCGA-06-0176 Missense BCL11A 1 broad.mit.edu No - BCL11A|chr2:60541333-60541333 Missense gcagaCgcagc C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +2 60542011 60542011 TCGA-06-0133 Missense BCL11A 1 broad.mit.edu No - BCL11A|chr2:60542011-60542011 Missense actacGgcttc G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +2 60542638 60542638 TCGA-02-0083 Missense BCL11A 1 broad.mit.edu No - BCL11A|chr2:60542638-60542638 Missense tgaatCcaatg C (TpC) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 60542986 60542986 TCGA-02-0028 Missense BCL11A 1 broad.mit.edu No - BCL11A|chr2:60542986-60542986 Missense aacacGcacag G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 102332064 102332064 TCGA-02-0007 Missense IL1RL1 1 BCM No + IL1RL1|chr2:102332064-102332064 Missense ccagaTtctgc T C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +2 127170093 127170093 TCGA-02-0114 Missense GYPC 1 genome.wustl.edu No + GYPC|chr2:127170093-127170093 Missense ccaagGgcacg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 189576409 189576409 TCGA-02-0043 Missense COL3A1 1 broad.mit.edu No + COL3A1|chr2:189576409-189576409 Missense tcaaaGgtgaa G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 189576733 189576733 TCGA-02-0083 Missense COL3A1 1 broad.mit.edu No + COL3A1|chr2:189576733-189576733 Missense tcgtgGtcttc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 191571219 191571219 TCGA-06-0185 Missense STAT1 1 BCM No - STAT1|chr2:191571219-191571219 Missense caagaAgatgt A T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +2 215948361 215948361 TCGA-02-0083 Missense FN1 1 broad.mit.edu No - FN1|chr2:215948361-215948361 Missense ttcagCcattg C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 215948606 215948606 TCGA-02-0010 Missense FN1 1 broad.mit.edu No - FN1|chr2:215948606-215948606 Missense tcgacGcctcc G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 215997243 215997243 TCGA-02-0114 Missense FN1 1 broad.mit.edu No - FN1|chr2:215997243-215997243 Missense tcttaCcattc C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 215998200 215998200 TCGA-02-0114 Missense FN1 1 broad.mit.edu No - FN1|chr2:215998200-215998200 Missense ctcacCcccag C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 219249113 219249113 TCGA-02-0014 Nonsense STK36 1 genome.wustl.edu No + STK36|chr2:219249113-219249113 Nonsense ctctgGtctgt G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 222055639 222055639 TCGA-02-0083 Missense EPHA4 1 genome.wustl.edu No - EPHA4|chr2:222055639-222055639 Missense tgctcCcctga C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 227368280 227368280 TCGA-02-0010 Missense IRS1 1 genome.wustl.edu No - IRS1|chr2:227368280-227368280 Missense gaaacGccaca G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 227370416 227370416 TCGA-02-0033 Missense IRS1 1 genome.wustl.edu No - IRS1|chr2:227370416-227370416 Missense ctcctCggatg C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +2 233961724 233961724 TCGA-02-0114 Missense DGKD 1 broad.mit.edu No + DGKD|chr2:233961724-233961724 Missense agggcGaacgc G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +2 240599706 240599706 TCGA-02-0099 Splice_site NDUFA10 1 genome.wustl.edu No - NDUFA10|chr2:240599706-240599706 Other atgagGtgagc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +3 366197 366197 TCGA-02-0114 Missense CHL1 1 broad.mit.edu No + CHL1|chr3:366197-366197 Missense aacagCcactc C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +3 27208682 27208682 TCGA-02-0010 Missense NEK10 1 genome.wustl.edu No - NEK10|chr3:27208682-27208682 Missense cgatgAtatca A G Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +3 30666785 30666785 TCGA-02-0014 Missense TGFBR2 1 broad.mit.edu No + TGFBR2|chr3:30666785-30666785 Missense agaacAtaaca A T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +3 37017543 37017543 TCGA-02-0083 Missense MLH1 1 genome.wustl.edu No + MLH1|chr3:37017543-37017543 Missense ttcgaGgtgag G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +3 37042321 37042321 TCGA-02-0083 Missense MLH1 1 genome.wustl.edu No + MLH1|chr3:37042321-37042321 Missense cccagGccatt G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +3 37056750 37056750 TCGA-02-0014 Missense MLH1 1 genome.wustl.edu No + MLH1|chr3:37056750-37056750 Missense acagcAtcaaa A G Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +3 41240577 41240577 TCGA-02-0028 Splice_site CTNNB1 1 genome.wustl.edu No + CTNNB1|chr3:41240577-41240577 Other tcaagGtttgt G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +3 46454625 46454625 TCGA-02-0114 Splice_site LTF 1 genome.wustl.edu No - LTF|chr3:46454625-46454625 Other tcgcaGgctaa G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +3 46471868 46471868 TCGA-02-0010 Missense LTF 1 genome.wustl.edu No - LTF|chr3:46471868-46471868 Missense tgtgtCgcctg C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +3 50288996 50288996 TCGA-02-0083 Missense SEMA3B 1 genome.wustl.edu No + SEMA3B|chr3:50288996-50288996 Missense aggtgGcagcg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +3 53188716 53188716 TCGA-06-0145 Missense PRKCD 1 BCM No + PRKCD|chr3:53188716-53188716 Missense aggggCgcgtc C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +3 129846483 129846483 TCGA-02-0099 Missense RPN1 1 genome.wustl.edu No - RPN1|chr3:129846483-129846483 Missense atttgGaagta G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +3 143659194 143659194 TCGA-02-0010 Nonsense ATR 1 broad.mit.edu No - ATR|chr3:143659194-143659194 Nonsense tttttCgaaga C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +3 172205794 172205794 TCGA-02-0014 Missense SLC2A2 1 broad.mit.edu No - SLC2A2|chr3:172205794-172205794 Missense atgtgGctcag G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +3 180399335 180399355 TCGA-06-0166 Inframe_Del PIK3CA 1 BCM No + PIK3CA|chr3:180399335-180399355 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +3 180399357 180399359 TCGA-02-0046 Inframe_Del PIK3CA 1 BCM No + PIK3CA|chr3:180399357-180399359 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +3 180399570 180399570 TCGA-02-0116 Missense PIK3CA 1 BCM No + PIK3CA|chr3:180399570-180399570 Missense aagacGacttt G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +3 180404247 180404247 TCGA-02-0047 Missense PIK3CA 1 BCM No + PIK3CA|chr3:180404247-180404247 Missense gtgaaTgtaaa T A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +3 180410674 180410674 TCGA-02-0037 Missense PIK3CA 1 BCM No + PIK3CA|chr3:180410674-180410674 Missense aacacTgtcca T C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +3 180434766 180434766 TCGA-06-0171 Missense PIK3CA 1 BCM, hgsc.bcm.edu No + PIK3CA|chr3:180434766-180434766 Missense aacaaAtgaat A G Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +3 187469360 187469361 TCGA-02-0054 Frameshift_Ins DGKG 1 BCM No - DGKG|chr3:187469360-187469361 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +3 197096286 197096286 TCGA-02-0043 Nonsense TNK2 1 broad.mit.edu No - TNK2|chr3:197096286-197096286 Nonsense gaaagCgactg C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +3 197098255 197098255 TCGA-06-0189 Nonsense TNK2 1 broad.mit.edu No - TNK2|chr3:197098255-197098255 Nonsense gtcgtGgatga G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +3 197106537 197106537 TCGA-02-0014 Missense TNK2 1 broad.mit.edu No - TNK2|chr3:197106537-197106537 Missense gcctgGctggg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +4 8633952 8633952 TCGA-02-0009 Missense GPR78 1 genome.wustl.edu No + GPR78|chr4:8633952-8633952 Missense gctacGccgga G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +4 54828591 54828591 TCGA-06-0174 Missense PDGFRA 1 BCM, genome.wustl.edu Yes + PDGFRA|chr4:54828591-54828591 Missense tcctgGctgaa G (other) C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +4 55288766 55288766 TCGA-02-0083 Missense KIT 1 BCM, broad.mit.edu, genome.wustl.edu Yes + KIT|chr4:55288766-55288766 Missense ctggaGctttc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +4 153463593 153463593 TCGA-02-0028 Missense FBXW7 1 broad.mit.edu, genome.wustl.edu Yes - FBXW7|chr4:153463593-153463593 Missense gagttGtgtgg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +4 153471357 153471357 TCGA-02-0010 Nonsense FBXW7 1 BCM, broad.mit.edu, genome.wustl.edu Yes - FBXW7|chr4:153471357-153471357 Nonsense ggaggCgagga C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +4 154436236 154436236 TCGA-02-0058 Missense TRIM2 1 BCM No + TRIM2|chr4:154436236-154436236 Missense ggcagCccatg C (other) T Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +4 158462195 158462197 TCGA-06-0213 Inframe_Del GRIA2 1 BCM No + GRIA2|chr4:158462195-158462197 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +5 1332521 1332521 TCGA-02-0010 Missense TERT 1 genome.wustl.edu No - TERT|chr5:1332521-1332521 Missense gcggcGccccg G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +5 1346683 1346683 TCGA-02-0043 Missense TERT 1 genome.wustl.edu No - TERT|chr5:1346683-1346683 Missense ccgagGaggag G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +5 67624894 67624894 TCGA-02-0003 Missense PIK3R1 1 genome.wustl.edu No + PIK3R1|chr5:67624894-67624894 Missense aagggGgaaat G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +5 67625310 67625312 TCGA-02-0021 Inframe_Del PIK3R1 1 genome.wustl.edu No + PIK3R1|chr5:67625310-67625312 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +5 67625370 67625372 TCGA-02-0028 Inframe_Del PIK3R1 1 genome.wustl.edu No + PIK3R1|chr5:67625370-67625372 Other Indel Indel Indel Somatic Unknown Somatic Verified OK Use_nonsilent Hypermutated +5 67626840 67626857 TCGA-06-0178 Inframe_Del PIK3R1 1 genome.wustl.edu No + PIK3R1|chr5:67626840-67626857 Other Indel Indel Indel Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +5 67626841 67626841 TCGA-02-0089 Missense PIK3R1 1 genome.wustl.edu No + PIK3R1|chr5:67626841-67626841 Missense aaattGacaaa G (GpA) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +5 67626855 67626855 TCGA-02-0074 Missense PIK3R1 1 genome.wustl.edu No + PIK3R1|chr5:67626855-67626855 Missense atgaaCagcat C (other) G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +5 67626884 67626885 TCGA-06-0129 Frameshift_Del PIK3R1 1 genome.wustl.edu No + PIK3R1|chr5:67626884-67626885 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +5 67626890 67626892 TCGA-02-0114 Inframe_Del PIK3R1 1 genome.wustl.edu No + PIK3R1|chr5:67626890-67626892 Other Indel Indel Indel Somatic Unknown Somatic Verified OK Use_nonsilent Hypermutated +5 67627006 67627008 TCGA-06-0133 Inframe_Del PIK3R1 1 genome.wustl.edu No + PIK3R1|chr5:67627006-67627008 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +5 96112135 96112135 TCGA-02-0043 Missense CAST 1 broad.mit.edu No + CAST|chr5:96112135-96112135 Missense gccggCtacct C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +5 149477555 149477555 TCGA-02-0114 Missense PDGFRB 1 BCM No - PDGFRB|chr5:149477555-149477555 Missense ccatcCttcgg C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +5 149495455 149495455 TCGA-02-0083 Missense PDGFRB 1 BCM No - PDGFRB|chr5:149495455-149495455 Missense aaatgGccaag G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +5 151029489 151029489 TCGA-02-0010 Missense SPARC 1 broad.mit.edu No - SPARC|chr5:151029489-151029489 Missense ctttgCcacaa C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +5 179624427 179624427 TCGA-02-0028 Missense MAPK9 1 broad.mit.edu No - MAPK9|chr5:179624427-179624427 Missense taaatGtgttt G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +5 179640065 179640065 TCGA-02-0014 Missense MAPK9 1 broad.mit.edu No - MAPK9|chr5:179640065-179640065 Missense gctctGgggcc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +5 179978410 179978410 TCGA-06-0201 Missense FLT4 1 genome.wustl.edu No - FLT4|chr5:179978410-179978410 Missense accgaGggcgg G (other) C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +6 36212465 36212465 TCGA-02-0083 Missense MAPK13 1 BCM No + MAPK13|chr6:36212465-36212465 Missense acgtgGtgacc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +6 37247171 37247171 TCGA-02-0011 Missense PIM1 1 BCM No + PIM1|chr6:37247171-37247171 Missense cctcaAtcgcg A G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +6 43083723 43083723 TCGA-02-0069 Missense PPP2R5D 1 broad.mit.edu No + PPP2R5D|chr6:43083723-43083723 Missense tctatGgcaag G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +6 56466808 56466808 TCGA-02-0114 Missense DST 1 genome.wustl.edu No - DST|chr6:56466808-56466808 Missense tgaaaGaaaaa G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +6 56534115 56534115 TCGA-02-0043 Missense DST 1 genome.wustl.edu No - DST|chr6:56534115-56534115 Missense agacaCctgca C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +6 56564994 56564994 TCGA-06-0125 Missense DST 1 genome.wustl.edu No - DST|chr6:56564994-56564994 Missense cttatCtgaac C (TpC) G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +6 56576971 56576971 TCGA-06-0145 Missense DST 1 genome.wustl.edu No - DST|chr6:56576971-56576971 Missense atatgGagtat G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +6 56580657 56580657 TCGA-02-0083 Missense DST 1 genome.wustl.edu No - DST|chr6:56580657-56580657 Missense tgatgGaaaca G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +6 56581605 56581605 TCGA-02-0010 Missense DST 1 genome.wustl.edu No - DST|chr6:56581605-56581605 Missense ctctgAgaagg A G Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +6 56591932 56591932 TCGA-02-0014 Missense DST 1 genome.wustl.edu No - DST|chr6:56591932-56591932 Missense agatgGtttta G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +6 56607311 56607311 TCGA-06-0148 Missense DST 1 genome.wustl.edu No - DST|chr6:56607311-56607311 Missense tggaaGgtcat G (other) C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +6 69760544 69760544 TCGA-02-0010 Missense BAI3 1 genome.wustl.edu No + BAI3|chr6:69760544-69760544 Missense aagggCaagtt C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +6 70149531 70149531 TCGA-02-0083 Missense BAI3 1 genome.wustl.edu No + BAI3|chr6:70149531-70149531 Missense atataCcaaat C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +6 94177152 94177152 TCGA-02-0083 Missense EPHA7 1 BCM No - EPHA7|chr6:94177152-94177152 Missense ctggtCcatta C (TpC) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +6 94177566 94177566 TCGA-02-0003 Missense EPHA7 1 BCM No - EPHA7|chr6:94177566-94177566 Missense gatacGaacat G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +6 108989360 108989360 TCGA-02-0083 Missense FOXO3 1 broad.mit.edu No + FOXO3|chr6:108989360-108989360 Missense ggagcGgcacg G (CpG) C Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +6 117769074 117769074 TCGA-02-0089 Missense ROS1 1 broad.mit.edu No - ROS1|chr6:117769074-117769074 Missense ccttgGttcca G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +6 117790458 117790458 TCGA-02-0114 Splice_site ROS1 1 broad.mit.edu No - ROS1|chr6:117790458-117790458 Other tccagGtatga G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +6 142441713 142441713 TCGA-06-0178 Missense NMBR 1 BCM No - NMBR|chr6:142441713-142441713 Missense taaccCcatgg C (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +6 142451232 142451232 TCGA-02-0010 Missense NMBR 1 BCM No - NMBR|chr6:142451232-142451232 Missense cctggCggccg C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +7 6005414 6005414 TCGA-02-0028 Nonsense PMS2 1 BCM No - PMS2|chr7:6005414-6005414 Nonsense tggtcCaggtc C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +7 15692146 15692146 TCGA-02-0028 Missense MEOX2 1 genome.wustl.edu No - MEOX2|chr7:15692146-15692146 Missense gggctCcagca C (TpC) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +7 17881916 17881916 TCGA-02-0083 Missense SNX13 1 BCM No - SNX13|chr7:17881916-17881916 Missense ggcaaCcttat C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +7 42046363 42046363 TCGA-02-0083 Missense GLI3 1 BCM No - GLI3|chr7:42046363-42046363 Missense aacagGcacca G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +7 55177569 55177569 TCGA-06-0237 Missense EGFR 1 BCM, broad.mit.edu, genome.wustl.edu Yes + EGFR|chr7:55177569-55177569 Missense ggtccTtggga T G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 55187768 55187768 TCGA-06-0154 Missense EGFR 1 BCM, broad.mit.edu Yes + EGFR|chr7:55187768-55187768 Missense ccgggCgctgc C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 55189204 55189204 TCGA-02-0021 Missense EGFR 1 BCM, broad.mit.edu, genome.wustl.edu Yes + EGFR|chr7:55189204-55189204 Missense tctgcCgcaaa C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 55189237 55189237 TCGA-02-0007 Missense EGFR 1 BCM, broad.mit.edu Yes + EGFR|chr7:55189237-55189237 Missense aggacAcctgc A C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 55189315 55189315 TCGA-02-0089 Missense EGFR 1 BCM, broad.mit.edu, genome.wustl.edu Yes + EGFR|chr7:55189315-55189315 Missense ttggtGccacc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 55189316 55189316 TCGA-02-0054 Missense EGFR 1 BCM, broad.mit.edu, genome.wustl.edu Yes + EGFR|chr7:55189316-55189316 Missense tggtgCcacct C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 55189316 55189316 TCGA-06-0156 Missense EGFR 1 broad.mit.edu Yes + EGFR|chr7:55189316-55189316 Missense tggtgCcacct C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 55189316 55189316 TCGA-06-0148 Missense EGFR 1 broad.mit.edu, genome.wustl.edu Yes + EGFR|chr7:55189316-55189316 Missense tggtgCcacct C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 55189316 55189316 TCGA-06-0157 Missense EGFR 1 BCM, broad.mit.edu, genome.wustl.edu Yes + EGFR|chr7:55189316-55189316 Missense tggtgCcacct C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 55200533 55200533 TCGA-02-0116 Missense EGFR 1 BCM, broad.mit.edu, genome.wustl.edu Yes + EGFR|chr7:55200533-55200533 Missense gcccgGcagga G (other) C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 55200537 55200537 TCGA-06-0125 Missense EGFR 1 BCM, broad.mit.edu, genome.wustl.edu Yes + EGFR|chr7:55200537-55200537 Missense ggcagGagtca G (GpA) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 55200537 55200537 TCGA-02-0046 Missense EGFR 1 BCM, broad.mit.edu, genome.wustl.edu, hgsc.bcm.edu Yes + EGFR|chr7:55200537-55200537 Missense ggcagGagtca G (GpA) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 55200603 55200603 TCGA-02-0003 Missense EGFR 1 BCM, broad.mit.edu, genome.wustl.edu, hgsc.bcm.edu Yes + EGFR|chr7:55200603-55200603 Missense cctgtGccatc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 55205721 55205721 TCGA-06-0156 Missense EGFR 1 BCM Yes + EGFR|chr7:55205721-55205721 Missense ctcctCctgcc C (TpC) T Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +7 55208201 55208201 TCGA-06-0185 Missense EGFR 1 BCM, genome.wustl.edu Yes + EGFR|chr7:55208201-55208201 Missense ggatgGtgggg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 55208288 55208288 TCGA-02-0010 Missense EGFR 1 BCM, broad.mit.edu, genome.wustl.edu Yes + EGFR|chr7:55208288-55208288 Missense cgctgCggagg C (CpG) G Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +7 79671071 79671071 TCGA-06-0209 Missense GNAI1 1 broad.mit.edu No + GNAI1|chr7:79671071-79671071 Missense tcaaaGatctt G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 93892889 93892889 TCGA-06-0126 Missense COL1A2 1 genome.wustl.edu No + COL1A2|chr7:93892889-93892889 Missense aggtcGcgatg G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 95676178 95676178 TCGA-02-0083 Missense SLC25A13 1 broad.mit.edu No - SLC25A13|chr7:95676178-95676178 Missense atattCcattt C (TpC) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +7 98391837 98391837 TCGA-02-0114 Missense TRRAP 1 genome.wustl.edu No + TRRAP|chr7:98391837-98391837 Missense agtggGagctg G (GpA) A Valid Valid Valid Validated OK Use_nonsilent Hypermutated +7 98400280 98400280 TCGA-02-0099 Missense TRRAP 1 genome.wustl.edu No + TRRAP|chr7:98400280-98400280 Missense agatgGtccgg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +7 98447697 98447697 TCGA-02-0010 Missense TRRAP 1 genome.wustl.edu No + TRRAP|chr7:98447697-98447697 Missense agaggAcacgt A G Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +7 99219625 99219625 TCGA-02-0099 Missense CYP3A4 1 genome.wustl.edu No - CYP3A4|chr7:99219625-99219625 Missense tcccaGacttg G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +7 104994978 104994978 TCGA-02-0083 Missense RINT1 1 BCM No + RINT1|chr7:104994978-104994978 Missense ttggcCtaata C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +7 116168234 116168234 TCGA-02-0114 Nonsense MET 1 BCM, genome.wustl.edu Yes + MET|chr7:116168234-116168234 Nonsense ggctgGtgcca G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +7 116199281 116199281 TCGA-02-0010 Splice_site MET 1 BCM Yes + MET|chr7:116199281-116199281 Other gaaggTatatt T C Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +7 116546849 116546849 TCGA-02-0021 Splice_site ST7 1 broad.mit.edu No + ST7|chr7:116546849-116546849 Other gttctAgatat A G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 116705516 116705516 TCGA-02-0099 Missense WNT2 1 BCM No - WNT2|chr7:116705516-116705516 Missense gtcagGactgc G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +7 137886376 137886376 TCGA-06-0171 Missense TRIM24 1 BCM No + TRIM24|chr7:137886376-137886376 Missense ccttcGtgcaa G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +7 150327287 150327287 TCGA-02-0114 Missense NOS3 1 genome.wustl.edu No + NOS3|chr7:150327287-150327287 Missense ttgggGgcctg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +7 150329333 150329333 TCGA-02-0114 Missense NOS3 1 genome.wustl.edu No + NOS3|chr7:150329333-150329333 Missense ccaggGggggc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +7 155288984 155288984 TCGA-06-0124 Missense SHH 1 BCM No - SHH|chr7:155288984-155288984 Missense tctacGtgatc G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +8 38391465 38391465 TCGA-02-0060 Missense FGFR1 1 BCM, broad.mit.edu, genome.wustl.edu Yes - FGFR1|chr8:38391465-38391465 Missense ataaaAagaca A G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +8 48873436 48873436 TCGA-02-0099 Missense PRKDC 1 genome.wustl.edu No - PRKDC|chr8:48873436-48873436 Missense aaattCcaatg C (TpC) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +8 48912493 48912493 TCGA-02-0114 Missense PRKDC 1 genome.wustl.edu No - PRKDC|chr8:48912493-48912493 Missense ggttaCcttca C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +8 57025643 57025643 TCGA-02-0083 Missense LYN 1 genome.wustl.edu No + LYN|chr8:57025643-57025643 Missense tgtggCcaaac C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +8 57241776 57241776 TCGA-02-0055 Missense PLAG1 1 BCM, broad.mit.edu, genome.wustl.edu Yes - PLAG1|chr8:57241776-57241776 Missense ctgatGgagtt G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +8 75920181 75920181 TCGA-02-0080 Missense PI15 1 broad.mit.edu No + PI15|chr8:75920181-75920181 Missense tttggGccact G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +8 75923999 75923999 TCGA-02-0010 Missense PI15 1 broad.mit.edu No + PI15|chr8:75923999-75923999 Missense atctgTgtttt T A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +8 91017009 91017009 TCGA-02-0099 Missense NBN 1 genome.wustl.edu No - NBN|chr8:91017009-91017009 Missense acaatCcttat C (TpC) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +8 93157428 93157428 TCGA-02-0083 Missense RUNX1T1 1 broad.mit.edu No - RUNX1T1|chr8:93157428-93157428 Missense ttggaGagcac G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +8 120014420 120014420 TCGA-02-0010 Missense TNFRSF11B 1 BCM No - TNFRSF11B|chr8:120014420-120014420 Missense aagggCgctac C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +9 21827925 21827925 TCGA-06-0156 Missense MTAP 1 broad.mit.edu No + MTAP|chr9:21827925-21827925 Missense cctcaGtcctt G (other) C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +9 21961053 21961053 TCGA-02-0010 Missense CDKN2A 1 BCM, genome.wustl.edu Yes - CDKN2A|chr9:21961053-21961053 Missense cggggCgcggc C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +9 21961124 21961125 TCGA-02-0083 Frameshift_Del CDKN2A 1 genome.wustl.edu Yes - CDKN2A|chr9:21961124-21961125 Other Indel Indel Indel Somatic Unknown Somatic Verified OK Use_nonsilent Hypermutated +9 21961127 21961128 TCGA-02-0083 Frameshift_Del CDKN2A 1 BCM Yes - CDKN2A|chr9:21961127-21961128 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +9 21964782 21964782 TCGA-02-0114 Nonsense CDKN2A 1 BCM, genome.wustl.edu Yes - CDKN2A|chr9:21964782-21964782 Nonsense gactgGctggc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +9 23691590 23691590 TCGA-02-0083 Missense ELAVL2 1 genome.wustl.edu No - ELAVL2|chr9:23691590-23691590 Missense aagggGtgtag G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +9 34542713 34542713 TCGA-02-0083 Missense CNTFR 1 BCM No - CNTFR|chr9:34542713-34542713 Missense ctggaCtgagg C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +9 36836902 36836902 TCGA-06-0214 Missense PAX5 1 genome.wustl.edu No - PAX5|chr9:36836902-36836902 Missense tccctAcagcc A G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +9 74974800 74974800 TCGA-02-0028 Missense ANXA1 1 BCM No + ANXA1|chr9:74974800-74974800 Missense caaagGagatt G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +9 93527042 93527042 TCGA-02-0083 Missense ROR2 1 BCM No - ROR2|chr9:93527042-93527042 Missense tgcggGaggag G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +9 93535351 93535351 TCGA-02-0010 Missense ROR2 1 BCM No - ROR2|chr9:93535351-93535351 Missense ccatcGcccgc G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +9 97249179 97249179 TCGA-06-0125 Nonsense PTCH1 1 BCM No - PTCH1|chr9:97249179-97249179 Nonsense accccCgaggg C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +9 109287936 109287936 TCGA-02-0010 Missense KLF4 1 genome.wustl.edu No - KLF4|chr9:109287936-109287936 Missense cggggCaccgc C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +9 109289919 109289919 TCGA-02-0010 Missense KLF4 1 genome.wustl.edu No - KLF4|chr9:109289919-109289919 Missense acgacGtgagc G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +9 116837402 116837402 TCGA-06-0188 Missense TNC 1 broad.mit.edu No - TNC|chr9:116837402-116837402 Missense ctactGaggtt G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +9 116878675 116878675 TCGA-02-0083 Missense TNC 1 broad.mit.edu No - TNC|chr9:116878675-116878675 Missense aacagGcctcg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +9 116888335 116888335 TCGA-02-0010 Missense TNC 1 broad.mit.edu No - TNC|chr9:116888335-116888335 Missense ggatcGccaat G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +9 134761810 134761810 TCGA-02-0114 Missense TSC1 1 genome.wustl.edu No - TSC1|chr9:134761810-134761810 Missense cagcaGcgagc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +9 135631024 135631024 TCGA-02-0114 Splice_site VAV2 1 genome.wustl.edu No - VAV2|chr9:135631024-135631024 Other caccaGccgcc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +9 138509420 138509420 TCGA-02-0114 Missense NOTCH1 1 genome.wustl.edu No - NOTCH1|chr9:138509420-138509420 Missense gctgaCcccgg C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +9 138510658 138510658 TCGA-02-0083 Missense NOTCH1 1 genome.wustl.edu No - NOTCH1|chr9:138510658-138510658 Missense gcctgGcggtg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 3814279 3814279 TCGA-02-0114 Missense KLF6 1 genome.wustl.edu No - KLF6|chr10:3814279-3814279 Missense ggaatCcgaac C (TpC) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 3817172 3817172 TCGA-02-0010 Missense KLF6 1 genome.wustl.edu No - KLF6|chr10:3817172-3817172 Missense ccaggAgctcc A G Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 8140468 8140468 TCGA-02-0010 Missense GATA3 1 genome.wustl.edu No + GATA3|chr10:8140468-8140468 Missense gccacGccagc G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 8155865 8155865 TCGA-02-0028 Missense GATA3 1 genome.wustl.edu No + GATA3|chr10:8155865-8155865 Missense gtcctCcctga C (TpC) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 12038348 12038348 TCGA-02-0083 Missense UPF2 1 broad.mit.edu No - UPF2|chr10:12038348-12038348 Missense tgttaGaagat G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 12199705 12199705 TCGA-02-0114 Missense DHTKD1 1 broad.mit.edu No + DHTKD1|chr10:12199705-12199705 Missense aaatgGcacca G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 12331616 12331616 TCGA-06-0141 Missense CDC123 1 broad.mit.edu No + CDC123|chr10:12331616-12331616 Missense acagtGaagtg G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 26865111 26865111 TCGA-06-0154 Missense APBB1IP 1 broad.mit.edu No + APBB1IP|chr10:26865111-26865111 Missense ttttaCgggct C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 29011215 29011215 TCGA-02-0114 Missense BAMBI 1 genome.wustl.edu No + BAMBI|chr10:29011215-29011215 Missense cagtgGgcacg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 31831344 31831344 TCGA-02-0114 Missense ZEB1 1 genome.wustl.edu No + ZEB1|chr10:31831344-31831344 Missense ttgaaGagttt G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 50497778 50497778 TCGA-02-0083 Missense CHAT 1 BCM No + CHAT|chr10:50497778-50497778 Missense ccaggGgctgc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 50505694 50505694 TCGA-06-0125 Missense CHAT 1 BCM No + CHAT|chr10:50505694-50505694 Missense ccgccGtctca G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 74826987 74826987 TCGA-02-0083 Missense ANXA7 1 broad.mit.edu No - ANXA7|chr10:74826987-74826987 Missense ttctgGgtatc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 76407199 76407199 TCGA-02-0114 Missense MYST4 1 broad.mit.edu No + MYST4|chr10:76407199-76407199 Missense cttggGaggta G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 76459177 76459177 TCGA-02-0043 Missense MYST4 1 broad.mit.edu No + MYST4|chr10:76459177-76459177 Missense agaggGaaacc G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 89643789 89643789 TCGA-06-0184 Missense PTEN 1 BCM, broad.mit.edu, genome.wustl.edu Yes + PTEN|chr10:89643789-89643789 Missense tatggGatttc G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89680799 89680800 TCGA-06-0197 Frameshift_Del PTEN 1 BCM Yes + PTEN|chr10:89680799-89680800 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89680800 89680801 TCGA-06-0157 Frameshift_Del PTEN 1 genome.wustl.edu Yes + PTEN|chr10:89680800-89680801 Other Indel Indel Indel Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +10 89680800 89680801 TCGA-06-0197 Frameshift_Del PTEN 1 genome.wustl.edu Yes + PTEN|chr10:89680800-89680801 Other Indel Indel Indel Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +10 89680805 89680806 TCGA-02-0052 Frameshift_Del PTEN 1 BCM, genome.wustl.edu Yes + PTEN|chr10:89680805-89680806 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89682772 89682772 TCGA-06-0190 Missense PTEN 1 BCM, broad.mit.edu, genome.wustl.edu Yes + PTEN|chr10:89682772-89682772 Missense gaagaCcataa C (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89682783 89682783 TCGA-02-0014 Missense PTEN 1 BCM, broad.mit.edu, genome.wustl.edu Yes + PTEN|chr10:89682783-89682783 Missense cccacCacagc C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 89682810 89682811 TCGA-06-0156 Frameshift_Del PTEN 1 BCM Yes + PTEN|chr10:89682810-89682811 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89682884 89682884 TCGA-06-0138 Nonsense PTEN 1 BCM, broad.mit.edu Yes + PTEN|chr10:89682884-89682884 Nonsense agggaCgaact C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89682884 89682884 TCGA-06-0176 Nonsense PTEN 1 BCM, broad.mit.edu Yes + PTEN|chr10:89682884-89682884 Nonsense agggaCgaact C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89682885 89682885 TCGA-02-0027 Frameshift_Del PTEN 1 BCM, broad.mit.edu, genome.wustl.edu Yes + PTEN|chr10:89682885-89682885 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89682887 89682887 TCGA-02-0055 Missense PTEN 1 BCM, broad.mit.edu, genome.wustl.edu Yes + PTEN|chr10:89682887-89682887 Missense gacgaActggt A G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89682891 89682891 TCGA-06-0187 Missense PTEN 1 BCM, broad.mit.edu, genome.wustl.edu Yes + PTEN|chr10:89682891-89682891 Missense aactgGtgtaa G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89682971 89682971 TCGA-02-0001 Frameshift_Del PTEN 1 genome.wustl.edu Yes + PTEN|chr10:89682971-89682971 Other Indel Indel Indel Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +10 89682972 89682972 TCGA-06-0195 Missense PTEN 1 BCM, broad.mit.edu, genome.wustl.edu Yes + PTEN|chr10:89682972-89682972 Missense agtaaGgacca G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89701862 89701862 TCGA-06-0166 Missense PTEN 1 BCM, broad.mit.edu, genome.wustl.edu Yes + PTEN|chr10:89701862-89701862 Missense agtaaCtattc C (other) G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89701862 89701862 TCGA-02-0021 Missense PTEN 1 BCM Yes + PTEN|chr10:89701862-89701862 Missense agtaaCtattc C (other) G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89701879 89701879 TCGA-02-0038 Missense PTEN 1 BCM, broad.mit.edu, genome.wustl.edu Yes + PTEN|chr10:89701879-89701879 Missense agaggCgctat C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89701893 89701893 TCGA-02-0099 Nonsense PTEN 1 broad.mit.edu, genome.wustl.edu Yes + PTEN|chr10:89701893-89701893 Nonsense tattaTtatag T G Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 89701940 89701940 TCGA-02-0007 Missense PTEN 1 BCM, broad.mit.edu, genome.wustl.edu Yes + PTEN|chr10:89701940-89701940 Missense ggcacTgttgt T C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89707658 89707658 TCGA-02-0113 Nonsense PTEN 1 BCM, genome.wustl.edu Yes + PTEN|chr10:89707658-89707658 Nonsense gacggGaagac G (GpA) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89707676 89707676 TCGA-02-0075 Missense PTEN 1 BCM, broad.mit.edu, genome.wustl.edu Yes + PTEN|chr10:89707676-89707676 Missense tgtacTttgag T C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89710659 89710659 TCGA-06-0124 Missense PTEN 1 BCM, genome.wustl.edu Yes + PTEN|chr10:89710659-89710659 Missense aaataCattct C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89710781 89710784 TCGA-06-0125 Frameshift_Del PTEN 1 BCM Yes + PTEN|chr10:89710781-89710784 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89710784 89710787 TCGA-06-0125 Frameshift_Del PTEN 1 genome.wustl.edu Yes + PTEN|chr10:89710784-89710787 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89710784 89710787 TCGA-06-0206 Frameshift_Del PTEN 1 genome.wustl.edu Yes + PTEN|chr10:89710784-89710787 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89710788 89710788 TCGA-02-0071 Nonsense PTEN 1 genome.wustl.edu Yes + PTEN|chr10:89710788-89710788 Nonsense tacttTaacaa T A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 89710797 89710797 TCGA-06-0188 Frameshift_Del PTEN 1 genome.wustl.edu Yes + PTEN|chr10:89710797-89710797 Other Indel Indel Indel Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +10 89710811 89710815 TCGA-06-0168 Frameshift_Del PTEN 1 genome.wustl.edu Yes + PTEN|chr10:89710811-89710815 Other Indel Indel Indel Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +10 89710832 89710832 TCGA-06-0213 Nonsense PTEN 1 broad.mit.edu, genome.wustl.edu Yes + PTEN|chr10:89710832-89710832 Nonsense ccaacCgatac C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 123288210 123288210 TCGA-02-0033 Missense FGFR2 1 genome.wustl.edu No - FGFR2|chr10:123288210-123288210 Missense gaaacCagcac C (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +10 124348403 124348403 TCGA-02-0028 Missense DMBT1 1 genome.wustl.edu No + DMBT1|chr10:124348403-124348403 Missense tgatgCcaatg C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 128009005 128009005 TCGA-02-0010 Missense ADAM12 1 genome.wustl.edu No - ADAM12|chr10:128009005-128009005 Missense tggggAcctct A G Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +10 129073048 129073048 TCGA-02-0010 Missense DOCK1 1 broad.mit.edu No + DOCK1|chr10:129073048-129073048 Missense gtggtCggagg C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +11 6426862 6426862 TCGA-06-0122 Missense TRIM3 1 broad.mit.edu No - TRIM3|chr11:6426862-6426862 Missense ccactGcttta G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +11 6434421 6434421 TCGA-02-0083 Missense TRIM3 1 broad.mit.edu No - TRIM3|chr11:6434421-6434421 Missense cggacGgcacg G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +11 6586561 6586561 TCGA-02-0010 Missense ILK 1 BCM No + ILK|chr11:6586561-6586561 Missense tgtggAcaaag A G Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +11 9552534 9552534 TCGA-06-0190 Missense WEE1 1 BCM No + WEE1|chr11:9552534-9552534 Missense gggcgGgcgaa G (other) A Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +11 18380975 18380975 TCGA-02-0083 Missense LDHA 1 genome.wustl.edu No + LDHA|chr11:18380975-18380975 Missense cttgaCctacg C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +11 18381850 18381850 TCGA-02-0014 Missense LDHA 1 genome.wustl.edu No + LDHA|chr11:18381850-18381850 Missense tggtgTctctc T C Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +11 107611687 107611687 TCGA-02-0010 Missense ATM 1 broad.mit.edu No + ATM|chr11:107611687-107611687 Missense tttacGgagct G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +11 107620796 107620796 TCGA-02-0010 Missense ATM 1 broad.mit.edu No + ATM|chr11:107620796-107620796 Missense ggctgTcaact T C Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +11 111095932 111095932 TCGA-02-0114 Missense SNF1LK2 1 broad.mit.edu No + SNF1LK2|chr11:111095932-111095932 Missense gcctgGggcag G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +11 111096956 111096956 TCGA-06-0176 Missense SNF1LK2 1 broad.mit.edu No + SNF1LK2|chr11:111096956-111096956 Missense gccggCggctc C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +11 118654485 118654485 TCGA-02-0083 Missense CBL 1 broad.mit.edu No + CBL|chr11:118654485-118654485 Missense tgaacCcatcg C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +11 118661372 118661372 TCGA-02-0083 Nonsense CBL 1 broad.mit.edu No + CBL|chr11:118661372-118661372 Nonsense ccctgGacagg G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +11 125008322 125008322 TCGA-06-0145 Missense CHEK1 1 BCM No + CHEK1|chr11:125008322-125008322 Missense taatcGtgagc G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +11 125028953 125028953 TCGA-02-0043 Splice_site CHEK1 1 BCM No + CHEK1|chr11:125028953-125028953 Other ctaagGtattt G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +11 128143273 128143273 TCGA-02-0114 Missense FLI1 1 broad.mit.edu No + FLI1|chr11:128143273-128143273 Missense gggcgGaggcg G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +11 128186040 128186040 TCGA-02-0009 Missense FLI1 1 broad.mit.edu No + FLI1|chr11:128186040-128186040 Missense ccaacGtcccc G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +12 6630758 6630758 TCGA-02-0083 Missense ING4 1 broad.mit.edu No - ING4|chr12:6630758-6630758 Missense ctcggGggaaa G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 6630798 6630798 TCGA-02-0114 Nonsense ING4 1 broad.mit.edu No - ING4|chr12:6630798-6630798 Nonsense gagtgGttcca G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 6647395 6647395 TCGA-02-0114 Missense ZNF384 1 genome.wustl.edu No - ZNF384|chr12:6647395-6647395 Missense cccagGcccag G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 6652748 6652748 TCGA-02-0114 Missense ZNF384 1 genome.wustl.edu No - ZNF384|chr12:6652748-6652748 Missense cagctCctacc C (TpC) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 9116623 9116623 TCGA-02-0028 Missense A2M 1 genome.wustl.edu No - A2M|chr12:9116623-9116623 Missense gcaaaTtccaa T C Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 9150104 9150104 TCGA-02-0024 Missense A2M 1 genome.wustl.edu No - A2M|chr12:9150104-9150104 Missense tctttGggcag G (other) C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +12 25289551 25289551 TCGA-02-0069 Missense KRAS 1 genome.wustl.edu No - KRAS|chr12:25289551-25289551 Missense agctgGtggcg G (other) C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +12 43212631 43212631 TCGA-02-0083 Missense NELL2 1 broad.mit.edu No - NELL2|chr12:43212631-43212631 Missense gtgaaGgtcag G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 54780232 54780232 TCGA-02-0083 Missense ERBB3 1 BCM No + ERBB3|chr12:54780232-54780232 Missense ccagaGccttt G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 56151942 56151942 TCGA-06-0133 Missense GLI1 1 broad.mit.edu No + GLI1|chr12:56151942-56151942 Missense tgtggCtattc C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +12 56288721 56288721 TCGA-02-0107 Missense DTX3 1 broad.mit.edu No + DTX3|chr12:56288721-56288721 Missense cacagGgagac G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +12 56407992 56407992 TCGA-02-0014 Missense CENTG1 1 BCM No - CENTG1|chr12:56407992-56407992 Missense gcaagGtcaag G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 56408033 56408033 TCGA-02-0043 Missense CENTG1 1 BCM No - CENTG1|chr12:56408033-56408033 Missense cgagaGtcaga G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 56444898 56444898 TCGA-02-0043 Missense CYP27B1 1 BCM, broad.mit.edu, genome.wustl.edu Yes - CYP27B1|chr12:56444898-56444898 Missense gtctgGggcgc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 56445112 56445112 TCGA-02-0080 Missense CYP27B1 1 BCM, genome.wustl.edu Yes - CYP27B1|chr12:56445112-56445112 Missense ttgtgCctggg C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +12 56482213 56482213 TCGA-02-0114 Nonsense AVIL 1 broad.mit.edu No - AVIL|chr12:56482213-56482213 Nonsense atttgGagtgt G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 63175611 63175611 TCGA-02-0114 Missense TBK1 1 broad.mit.edu No + TBK1|chr12:63175611-63175611 Missense cagacGcatgg G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 67516737 67516737 TCGA-02-0085 Missense MDM2 1 broad.mit.edu No + MDM2|chr12:67516737-67516737 Missense ctgtgTatcag T C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +12 108019967 108019967 TCGA-02-0083 Missense UNG 1 BCM No + UNG|chr12:108019967-108019967 Missense gcgtgGctggg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 111372572 111372572 TCGA-02-0028 Missense PTPN11 1 genome.wustl.edu No + PTPN11|chr12:111372572-111372572 Missense gagggGagaaa G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 111395220 111395220 TCGA-06-0171 Missense PTPN11 1 genome.wustl.edu No + PTPN11|chr12:111395220-111395220 Missense aacatCctgcc C (TpC) G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +12 117103657 117103657 TCGA-02-0075 Missense TAOK3 1 broad.mit.edu No - TAOK3|chr12:117103657-117103657 Missense agcacCgcctc C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +12 119280036 119280036 TCGA-02-0083 Missense MSI1 1 broad.mit.edu No - MSI1|chr12:119280036-119280036 Missense agtgtGtgaaa G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 119286911 119286911 TCGA-02-0114 Missense MSI1 1 broad.mit.edu No - MSI1|chr12:119286911-119286911 Missense ggcgaGcacag G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 121222973 121222973 TCGA-02-0083 Nonsense IL31 1 genome.wustl.edu No - IL31|chr12:121222973-121222973 Nonsense gttttCagagt C (TpC) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 131012246 131012246 TCGA-02-0083 Nonsense EP400 1 BCM No + EP400|chr12:131012246-131012246 Nonsense agatgCaggct C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 131076211 131076211 TCGA-02-0114 Missense EP400 1 BCM No + EP400|chr12:131076211-131076211 Missense gtgatGcagac G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +12 131094732 131094732 TCGA-02-0114 Missense EP400 1 BCM No + EP400|chr12:131094732-131094732 Missense tgcagGagagg G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +13 27829760 27829760 TCGA-06-0173 Missense FLT1 1 BCM No - FLT1|chr13:27829760-27829760 Missense atgaaGgtgtc G (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +13 31788650 31788650 TCGA-02-0010 Missense BRCA2 1 broad.mit.edu No + BRCA2|chr13:31788650-31788650 Missense gacacGctgca G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +13 31809250 31809250 TCGA-06-0169 Missense BRCA2 1 broad.mit.edu No + BRCA2|chr13:31809250-31809250 Missense acgaaCccatt C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +13 31809542 31809542 TCGA-02-0014 Missense BRCA2 1 broad.mit.edu No + BRCA2|chr13:31809542-31809542 Missense ggaaaTcaagc T G Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +13 47834984 47834984 TCGA-06-0176 Nonsense RB1 1 BCM, broad.mit.edu Yes + RB1|chr13:47834984-47834984 Nonsense cacctCgaaca C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +13 47835032 47835069 TCGA-02-0055 Frameshift_Del RB1 1 BCM, genome.wustl.edu Yes + RB1|chr13:47835032-47835069 Other Indel Indel Indel Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +13 47845597 47845597 TCGA-06-0195 Nonsense RB1 1 BCM, broad.mit.edu Yes + RB1|chr13:47845597-47845597 Nonsense gtgatCaacct C (TpC) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +13 47925250 47925250 TCGA-02-0052 Splice_site RB1 1 BCM, broad.mit.edu, genome.wustl.edu Yes + RB1|chr13:47925250-47925250 Other atatgTaagca T C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +13 47928486 47928486 TCGA-06-0213 Missense RB1 1 BCM Yes + RB1|chr13:47928486-47928486 Missense aaaaaGgttag G (other) C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +13 47931824 47931824 TCGA-06-0184 Splice_site RB1 1 BCM, broad.mit.edu Yes + RB1|chr13:47931824-47931824 Other ccacaGtgtat G (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +13 47931840 47931840 TCGA-06-0184 Missense RB1 1 BCM, broad.mit.edu Yes + RB1|chr13:47931840-47931840 Missense agcctAtctcc A T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +13 47931968 47931968 TCGA-02-0033 Nonsense RB1 1 BCM, genome.wustl.edu Yes + RB1|chr13:47931968-47931968 Nonsense tggacCaagta C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +13 47937375 47937375 TCGA-02-0007 Nonsense RB1 1 BCM, broad.mit.edu, genome.wustl.edu Yes + RB1|chr13:47937375-47937375 Nonsense ttcctCgaagc C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +13 47945496 47945496 TCGA-06-0206 Splice_site RB1 1 BCM, broad.mit.edu Yes + RB1|chr13:47945496-47945496 Other tgacaGaatct G (GpA) C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +13 94484895 94484895 TCGA-02-0083 Missense ABCC4 1 broad.mit.edu No - ABCC4|chr13:94484895-94484895 Missense gcaagGcagaa G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +13 94613377 94613377 TCGA-02-0037 Missense ABCC4 1 broad.mit.edu No - ABCC4|chr13:94613377-94613377 Missense attcaGgtaaa G (other) C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +13 113008879 113008879 TCGA-02-0083 Missense LAMP1 1 broad.mit.edu No + LAMP1|chr13:113008879-113008879 Missense cttctCtgctg C (TpC) G Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +13 113021973 113021973 TCGA-02-0116 Splice_site LAMP1 1 broad.mit.edu No + LAMP1|chr13:113021973-113021973 Other acacgGtaggg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +14 55148856 55148856 TCGA-02-0083 Missense KTN1 1 broad.mit.edu No + KTN1|chr14:55148856-55148856 Missense ttaggGaaaga G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +14 59263887 59263887 TCGA-02-0021 Missense RTN1 1 broad.mit.edu No - RTN1|chr14:59263887-59263887 Missense ggccgCggagg C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +14 94150664 94150664 TCGA-06-0209 Missense SERPINA3 1 genome.wustl.edu No + SERPINA3|chr14:94150664-94150664 Missense cacacGtggac G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +14 101617806 101617806 TCGA-02-0010 Missense HSP90AA1 1 BCM No - HSP90AA1|chr14:101617806-101617806 Missense agtagActaat A G Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +14 101675353 101675353 TCGA-02-0083 Missense HSP90AA1 1 BCM No - HSP90AA1|chr14:101675353-101675353 Missense cgccgCcgttt C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +15 32316305 32316305 TCGA-06-0133 Missense SLC12A6 1 BCM No - SLC12A6|chr15:32316305-32316305 Missense agcatGacagt G (GpA) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +15 32317006 32317006 TCGA-02-0102 Missense SLC12A6 1 BCM No - SLC12A6|chr15:32317006-32317006 Missense agtagCccaat C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +15 55330914 55330914 TCGA-02-0114 Splice_site TCF12 1 BCM No + TCF12|chr15:55330914-55330914 Other ccctgGtaaga G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +15 55341606 55341607 TCGA-02-0114 Frameshift_Del TCF12 1 BCM No + TCF12|chr15:55341606-55341607 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +15 55342601 55342601 TCGA-06-0128 Splice_site TCF12 1 BCM No + TCF12|chr15:55342601-55342601 Other tgttaGgtggc G (other) C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +15 72077554 72077554 TCGA-02-0083 Missense PML 1 genome.wustl.edu No + PML|chr15:72077554-72077554 Missense cctggCcccta C (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +15 77008808 77008808 TCGA-02-0043 Splice_site CTSH 1 genome.wustl.edu No - CTSH|chr15:77008808-77008808 Other gcaagGtaact G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +15 82233332 82233332 TCGA-02-0028 Nonsense ADAMTSL3 1 BCM No + ADAMTSL3|chr15:82233332-82233332 Nonsense gcttgGggcga G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +15 82481336 82481336 TCGA-02-0083 Missense ADAMTSL3 1 BCM No + ADAMTSL3|chr15:82481336-82481336 Missense tccggGactgc G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +15 86528499 86528499 TCGA-02-0043 Missense NTRK3 1 genome.wustl.edu No - NTRK3|chr15:86528499-86528499 Missense gctcaAcgccg A G Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +15 88818012 88818012 TCGA-02-0083 Missense IQGAP1 1 BCM No + IQGAP1|chr15:88818012-88818012 Missense gcgctCccaca C (TpC) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +15 88818856 88818856 TCGA-02-0075 Missense IQGAP1 1 BCM No + IQGAP1|chr15:88818856-88818856 Missense taaccAgcagc A G Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +15 89225202 89225202 TCGA-02-0010 Missense FURIN 1 genome.wustl.edu No + FURIN|chr15:89225202-89225202 Missense gcaccGcccct G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +15 89225687 89225687 TCGA-02-0028 Missense FURIN 1 genome.wustl.edu No + FURIN|chr15:89225687-89225687 Missense aggggCcggcc C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +15 89225991 89225991 TCGA-02-0010 Missense FURIN 1 genome.wustl.edu No + FURIN|chr15:89225991-89225991 Missense ccgtgGcctca G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +16 2038750 2038750 TCGA-02-0047 Missense TSC2 1 genome.wustl.edu No + TSC2|chr16:2038750-2038750 Missense aaataCtgaga C (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +16 2064367 2064367 TCGA-02-0010 Missense TSC2 1 genome.wustl.edu No + TSC2|chr16:2064367-2064367 Missense tggccGtccca G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +16 2070190 2070190 TCGA-02-0010 Missense TSC2 1 genome.wustl.edu No + TSC2|chr16:2070190-2070190 Missense ttggcGccctg G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +16 2290056 2290056 TCGA-02-0043 Missense ABCA3 1 genome.wustl.edu No - ABCA3|chr16:2290056-2290056 Missense tgaagCcgagc C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +16 11256592 11256592 TCGA-02-0114 Missense SOCS1 1 genome.wustl.edu No - SOCS1|chr16:11256592-11256592 Missense ctgggGgcccc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +16 24138810 24138810 TCGA-06-0185 Missense PRKCB1 1 broad.mit.edu No + PRKCB1|chr16:24138810-24138810 Missense acttcGacaaa G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +16 49341571 49341571 TCGA-02-0114 Missense CYLD 1 genome.wustl.edu No + CYLD|chr16:49341571-49341571 Missense gttagCagaga C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +16 65554895 65554895 TCGA-06-0139 Missense CES3 1 genome.wustl.edu No + CES3|chr16:65554895-65554895 Missense cacagGtgcct G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +16 65555760 65555760 TCGA-02-0114 Splice_site CES3 1 genome.wustl.edu No + CES3|chr16:65555760-65555760 Other ccccaGcactg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +16 73821184 73821184 TCGA-06-0176 Missense BCAR1 1 BCM No - BCAR1|chr16:73821184-73821184 Missense tgtggCgcaca C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +16 73821401 73821401 TCGA-02-0099 Missense BCAR1 1 BCM No - BCAR1|chr16:73821401-73821401 Missense gactgGaacag G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 7514728 7514728 TCGA-02-0024 Nonsense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7514728-7514728 Nonsense tgttcCgagag C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7517636 7517636 TCGA-06-0128 Frameshift_Del TP53 1 BCM Yes - TP53|chr17:7517636-7517636 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7517651 7517651 TCGA-06-0188 Missense TP53 1 BCM, genome.wustl.edu Yes - TP53|chr17:7517651-7517651 Missense cctagCactgc C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7517652 7517652 TCGA-06-0188 Splice_site TP53 1 BCM, genome.wustl.edu Yes - TP53|chr17:7517652-7517652 Other tcctaGcactg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7517819 7517819 TCGA-02-0003 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7517819-7517819 Missense gagacCggcgc C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7517819 7517819 TCGA-02-0046 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7517819-7517819 Missense gagacCggcgc C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7517821 7517821 TCGA-06-0195 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7517821-7517821 Missense gagagAccggc A C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7517830 7517830 TCGA-02-0001 Missense TP53 1 BCM, genome.wustl.edu Yes - TP53|chr17:7517830-7517830 Missense ctgtcCtggga C (other) G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7517831 7517831 TCGA-02-0083 Missense TP53 1 BCM, genome.wustl.edu Yes - TP53|chr17:7517831-7517831 Missense cctgtCctggg C (TpC) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 7517836 7517836 TCGA-02-0114 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7517836-7517836 Missense ttgtgCctgtc C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 7517845 7517845 TCGA-06-0206 Missense TP53 1 BCM, genome.wustl.edu Yes - TP53|chr17:7517845-7517845 Missense ggtgcGtgttt G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7517846 7517846 TCGA-02-0080 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7517846-7517846 Missense aggtgCgtgtt C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7517846 7517846 TCGA-02-0010 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7517846-7517846 Missense aggtgCgtgtt C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 7517849 7517849 TCGA-02-0058 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7517849-7517849 Missense ttgagGtgcgt G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7517854 7517854 TCGA-06-0197 Missense TP53 1 broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7517854-7517854 Missense cagctTtgagg T C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7517867 7517867 TCGA-06-0195 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7517867-7517867 Missense tactgGgacgg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7518234 7518234 TCGA-02-0034 Missense TP53 1 BCM, genome.wustl.edu Yes - TP53|chr17:7518234-7518234 Missense cactgGaagac G (GpA) C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7518263 7518263 TCGA-02-0033 Missense TP53 1 BCM, genome.wustl.edu Yes - TP53|chr17:7518263-7518263 Missense gaaccGgaggc G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7518263 7518263 TCGA-06-0241 Missense TP53 1 BCM, genome.wustl.edu Yes - TP53|chr17:7518263-7518263 Missense gaaccGgaggc G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7518273 7518273 TCGA-06-0190 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7518273-7518273 Missense tgggcGgcatg G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7518303 7518303 TCGA-02-0074 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7518303-7518303 Missense actacAactac A G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7518928 7518928 TCGA-02-0055 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7518928-7518928 Missense atagtGtggtg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7518937 7518937 TCGA-02-0054 Nonsense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7518937-7518937 Nonsense cttttCgacat C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7518974 7518975 TCGA-02-0014 Frameshift_Del TP53 1 genome.wustl.edu Yes - TP53|chr17:7518974-7518975 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 7518990 7518990 TCGA-06-0237 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7518990-7518990 Missense tcttaTccgag T C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7519120 7519120 TCGA-02-0089 Missense TP53 1 broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7519120-7519120 Missense cccacCatgag C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7519121 7519121 TCGA-02-0003 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7519121-7519121 Missense ccccaCcatga C (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7519126 7519126 TCGA-02-0046 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7519126-7519126 Missense gctgcCcccac C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7519128 7519128 TCGA-02-0114 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7519128-7519128 Missense gcgctGccccc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 7519131 7519131 TCGA-02-0010 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7519131-7519131 Missense gaggcGctgcc G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 7519131 7519131 TCGA-02-0037 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7519131-7519131 Missense gaggcGctgcc G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7519132 7519132 TCGA-02-0011 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7519132-7519132 Missense tgaggCgctgc C (CpG) G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7519182 7519182 TCGA-06-0130 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7519182-7519182 Missense cgtccGcgcca G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7519182 7519182 TCGA-06-0184 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7519182-7519182 Missense cgtccGcgcca G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7519237 7519238 TCGA-06-0221 Frameshift_Del TP53 1 genome.wustl.edu Yes - TP53|chr17:7519237-7519238 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7519251 7519251 TCGA-02-0034 Missense TP53 1 BCM Yes - TP53|chr17:7519251-7519251 Missense gttttGccaac G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7519267 7519267 TCGA-02-0075 Missense TP53 1 BCM, broad.mit.edu, genome.wustl.edu Yes - TP53|chr17:7519267-7519267 Missense ctgccCtcaac C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 7520118 7520122 TCGA-06-0156 Frameshift_Del TP53 1 BCM Yes - TP53|chr17:7520118-7520122 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 8051651 8051651 TCGA-02-0114 Missense AURKB 1 broad.mit.edu No - AURKB|chr17:8051651-8051651 Missense agaagGtgatg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 19225977 19225977 TCGA-02-0014 Missense MAPK7 1 genome.wustl.edu No + MAPK7|chr17:19225977-19225977 Missense cccctGcccca G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 26574560 26574598 TCGA-02-0001 Splice_site NF1 1 genome.wustl.edu No + NF1|chr17:26574560-26574598 Other atgctAgtaac A Indel Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +17 26580304 26580304 TCGA-02-0075 Nonsense NF1 1 genome.wustl.edu No + NF1|chr17:26580304-26580304 Nonsense ttgggGgagtg G (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 26581118 26581118 TCGA-02-0028 Missense NF1 1 genome.wustl.edu No + NF1|chr17:26581118-26581118 Missense ggtcaGgtaag G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 26609550 26609550 TCGA-02-0107 Missense NF1 1 genome.wustl.edu No + NF1|chr17:26609550-26609550 Missense ctcagAtttat A T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 26610175 26610175 TCGA-02-0033 Splice_site NF1 1 genome.wustl.edu No + NF1|chr17:26610175-26610175 Other gtgtaGatact G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 26610182 26610182 TCGA-06-0190 Missense NF1 1 genome.wustl.edu No + NF1|chr17:26610182-26610182 Missense tacttCagagt C (TpC) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 26612877 26612877 TCGA-06-0201 Nonsense NF1 1 genome.wustl.edu No + NF1|chr17:26612877-26612877 Nonsense gaagaCgacct C (CpG) T Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +17 26677105 26677108 TCGA-02-0107 Frameshift_Del NF1 1 genome.wustl.edu No + NF1|chr17:26677105-26677108 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 26677114 26677114 TCGA-02-0115 Nonsense NF1 1 genome.wustl.edu No + NF1|chr17:26677114-26677114 Nonsense aagtgGtttgt G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 26678919 26678919 TCGA-06-0214 Missense NF1 1 genome.wustl.edu No + NF1|chr17:26678919-26678919 Missense caaaaGatgtc G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 26678984 26678984 TCGA-06-0201 Splice_site NF1 1 genome.wustl.edu No + NF1|chr17:26678984-26678984 Other ttacgGtaggt G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 26686128 26686128 TCGA-06-0124 Nonsense NF1 1 genome.wustl.edu No + NF1|chr17:26686128-26686128 Nonsense aaaaaCagatg C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 26687479 26687479 TCGA-02-0086 Frameshift_Del NF1 1 genome.wustl.edu No + NF1|chr17:26687479-26687479 Other Indel Indel Indel Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +17 26688511 26688511 TCGA-02-0028 Splice_site NF1 1 genome.wustl.edu No + NF1|chr17:26688511-26688511 Other ttacaGaagag G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 26689934 26689934 TCGA-06-0184 Frameshift_Del NF1 1 genome.wustl.edu No + NF1|chr17:26689934-26689934 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 26691678 26691678 TCGA-02-0028 Nonsense NF1 1 genome.wustl.edu No + NF1|chr17:26691678-26691678 Nonsense ttttgGgtagc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 26691722 26691722 TCGA-06-0124 Nonsense NF1 1 genome.wustl.edu No + NF1|chr17:26691722-26691722 Nonsense gtattCagcag C (TpC) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 26691733 26691733 TCGA-02-0010 Missense NF1 1 genome.wustl.edu No + NF1|chr17:26691733-26691733 Missense gtaccGcactt G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 26691739 26691739 TCGA-02-0086 Frameshift_Del NF1 1 genome.wustl.edu No + NF1|chr17:26691739-26691739 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 35116841 35116841 TCGA-02-0080 Missense ERBB2 1 genome.wustl.edu No + ERBB2|chr17:35116841-35116841 Missense ccaccTctacc T ? Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 35116922 35116922 TCGA-02-0115 Splice_site ERBB2 1 genome.wustl.edu No + ERBB2|chr17:35116922-35116922 Other gcaggTgaggc T A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 35119867 35119867 TCGA-02-0069 Missense ERBB2 1 genome.wustl.edu No + ERBB2|chr17:35119867-35119867 Missense cagtgAcgcgc A T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 35121736 35121736 TCGA-02-0089 Missense ERBB2 1 genome.wustl.edu No + ERBB2|chr17:35121736-35121736 Missense gatccTgcacc T C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 35121760 35121760 TCGA-02-0116 Missense ERBB2 1 genome.wustl.edu No + ERBB2|chr17:35121760-35121760 Missense tgcacAaccaa A G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 35121767 35121767 TCGA-02-0089 Missense ERBB2 1 genome.wustl.edu No + ERBB2|chr17:35121767-35121767 Missense ccaagAggtga A G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 35121782 35121782 TCGA-02-0115 Missense ERBB2 1 genome.wustl.edu No + ERBB2|chr17:35121782-35121782 Missense agaggAtggaa A G Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 35121805 35121805 TCGA-02-0115 Missense ERBB2 1 genome.wustl.edu No + ERBB2|chr17:35121805-35121805 Missense agaagTgcagc T A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 35133731 35133731 TCGA-02-0107 Missense ERBB2 1 genome.wustl.edu No + ERBB2|chr17:35133731-35133731 Missense tccagTggcca T A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 35134527 35134527 TCGA-02-0069 Missense ERBB2 1 genome.wustl.edu No + ERBB2|chr17:35134527-35134527 Missense tggtgTgggct T C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 35134527 35134527 TCGA-02-0113 Missense ERBB2 1 genome.wustl.edu No + ERBB2|chr17:35134527-35134527 Missense tggtgTgggct T C Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +17 37735120 37735121 TCGA-02-0114 Frameshift_Del STAT3 1 BCM No - STAT3|chr17:37735120-37735121 Other Indel Indel Indel Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 42723325 42723325 TCGA-06-0141 Missense ITGB3 1 broad.mit.edu No + ITGB3|chr17:42723325-42723325 Missense aaatcCgttct C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 42724694 42724694 TCGA-02-0083 Missense ITGB3 1 broad.mit.edu No + ITGB3|chr17:42724694-42724694 Missense gtgtgGggtat G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 42742529 42742529 TCGA-02-0010 Missense ITGB3 1 broad.mit.edu No + ITGB3|chr17:42742529-42742529 Missense agaggCcacgt C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 45539183 45539183 TCGA-02-0043 Missense PDK2 1 BCM No + PDK2|chr17:45539183-45539183 Missense ccaacCcagcc C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 45619111 45619111 TCGA-02-0043 Missense COL1A1 1 BCM No - COL1A1|chr17:45619111-45619111 Missense ccaccCtcaag C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 45623200 45623200 TCGA-02-0083 Missense COL1A1 1 BCM No - COL1A1|chr17:45623200-45623200 Missense ctggcCctgct C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 45628312 45628312 TCGA-02-0083 Missense COL1A1 1 BCM No - COL1A1|chr17:45628312-45628312 Missense gtcctCctggc C (TpC) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 45630091 45630091 TCGA-02-0043 Splice_site COL1A1 1 BCM No - COL1A1|chr17:45630091-45630091 Other atgatGtaagt G (other) Indel Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 62215542 62215542 TCGA-02-0083 Missense PRKCA 1 broad.mit.edu No + PRKCA|chr17:62215542-62215542 Missense agccaCcattc C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 63463748 63463748 TCGA-06-0141 Missense KPNA2 1 broad.mit.edu No + KPNA2|chr17:63463748-63463748 Missense tgcccGtcttc G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +17 74363373 74363373 TCGA-02-0083 Nonsense TIMP2 1 BCM No - TIMP2|chr17:74363373-74363373 Nonsense ccaagCaggag C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +17 74479447 74479447 TCGA-02-0028 Missense LGALS3BP 1 broad.mit.edu No - LGALS3BP|chr17:74479447-74479447 Missense acaaaGccctg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +18 41700876 41700876 TCGA-02-0083 Missense KIAA1632 1 BCM No - KIAA1632|chr18:41700876-41700876 Missense tttcaGcagcc G (other) C Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +18 42074101 42074101 TCGA-02-0099 Missense C18orf25 1 genome.wustl.edu No + C18orf25|chr18:42074101-42074101 Missense cacagGagcta G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +18 46858703 46858703 TCGA-02-0028 Nonsense SMAD4 1 broad.mit.edu No + SMAD4|chr18:46858703-46858703 Nonsense ggctgGggacc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +18 58790915 58790915 TCGA-06-0176 Missense PHLPP 1 broad.mit.edu No + PHLPP|chr18:58790915-58790915 Missense tgtcaTgcaaa T C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +18 59136627 59136627 TCGA-02-0089 Missense BCL2 1 genome.wustl.edu No - BCL2|chr18:59136627-59136627 Missense ggcctGcgctc G (other) A Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +19 7244845 7244845 TCGA-06-0147 Missense INSR 1 BCM No - INSR|chr19:7244845-7244845 Missense tggccGcgctg G (CpG) A Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +19 40903582 40903582 TCGA-02-0057 Missense MLL4 1 broad.mit.edu No + MLL4|chr19:40903582-40903582 Missense tccaaCcccca C (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +19 40908522 40908522 TCGA-02-0114 Missense MLL4 1 broad.mit.edu No + MLL4|chr19:40908522-40908522 Missense gcccaGctatc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +19 40916372 40916372 TCGA-02-0010 Missense MLL4 1 broad.mit.edu No + MLL4|chr19:40916372-40916372 Missense cagtgCgtggg C (CpG) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +19 46435815 46435815 TCGA-02-0083 Missense AXL 1 genome.wustl.edu No + AXL|chr19:46435815-46435815 Missense acaccCcttat C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +19 50482763 50482763 TCGA-06-0147 Splice_site MARK4 1 BCM No + MARK4|chr19:50482763-50482763 Other cccccGtgagt G (CpG) A Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +19 51892838 51892838 TCGA-02-0080 Missense PRKD2 1 broad.mit.edu No - PRKD2|chr19:51892838-51892838 Missense acaagGacacg G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +19 52115128 52115128 TCGA-02-0083 Nonsense GRLF1 1 BCM No + GRLF1|chr19:52115128-52115128 Nonsense ccttgGgaaga G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +19 54150026 54150026 TCGA-02-0055 Missense BAX 1 genome.wustl.edu No + BAX|chr19:54150026-54150026 Missense cagagGcgggg G (other) A Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +19 56195600 56195600 TCGA-02-0028 Missense KLK8 1 broad.mit.edu No - KLK8|chr19:56195600-56195600 Missense ccaacCccatt C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +20 10581166 10581166 TCGA-02-0043 Missense JAG1 1 BCM No - JAG1|chr20:10581166-10581166 Missense tgagcCctggc C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +20 29871752 29871752 TCGA-02-0043 Missense MYLK2 1 broad.mit.edu No + MYLK2|chr20:29871752-29871752 Missense aactaGcagcc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +20 30361377 30361377 TCGA-02-0083 Missense KIF3B 1 BCM No + KIF3B|chr20:30361377-30361377 Missense agaacCccaaa C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +20 39177465 39177465 TCGA-02-0083 Missense TOP1 1 genome.wustl.edu No + TOP1|chr20:39177465-39177465 Missense gcagcCcgagg C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +20 56849073 56849073 TCGA-02-0010 Missense GNAS 1 BCM No + GNAS|chr20:56849073-56849073 Missense ccgacGcctcc G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +20 56863479 56863479 TCGA-02-0083 Missense GNAS 1 BCM No + GNAS|chr20:56863479-56863479 Missense ggatgCctccg C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +21 45131203 45131203 TCGA-02-0014 Missense ITGB2 1 broad.mit.edu No - ITGB2|chr21:45131203-45131203 Missense gggcaCcgtgg C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +21 45144662 45144662 TCGA-06-0158 Splice_site ITGB2 1 broad.mit.edu No - ITGB2|chr21:45144662-45144662 Other aattcGtaagt G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +21 46358384 46358384 TCGA-02-0028 Missense COL6A2 1 genome.wustl.edu No + COL6A2|chr21:46358384-46358384 Missense tgggcCctctg C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +22 16455484 16455484 TCGA-02-0083 Missense ATP6V1E1 1 broad.mit.edu No - ATP6V1E1|chr22:16455484-16455484 Missense tccggGgagcc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +22 16590158 16590158 TCGA-02-0083 Missense BCL2L13 1 BCM No + BCL2L13|chr22:16590158-16590158 Missense tgaggGcaagt G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +22 21853559 21853559 TCGA-02-0083 Missense BCR 1 genome.wustl.edu No + BCR|chr22:21853559-21853559 Missense cagccGcgtcg G (CpG) A Somatic Unknown Somatic Verified OK Use_nonsilent Hypermutated +22 21926057 21926057 TCGA-02-0102 Missense BCR 1 genome.wustl.edu No + BCR|chr22:21926057-21926057 Missense accaaGatggg G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +22 26476992 26476992 TCGA-02-0028 Missense MN1 1 genome.wustl.edu No - MN1|chr22:26476992-26476992 Missense gtgacGccaag G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +22 37019247 37019247 TCGA-02-0010 Missense CSNK1E 1 broad.mit.edu No - CSNK1E|chr22:37019247-37019247 Missense atctcGggaag G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +22 39894533 39894533 TCGA-02-0114 Missense EP300 1 BCM No + EP300|chr22:39894533-39894533 Missense taaaaCcaggc C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +22 39903299 39903299 TCGA-06-0133 Missense EP300 1 BCM No + EP300|chr22:39903299-39903299 Missense cccctCccaat C (TpC) T Somatic Unknown Somatic Verified OK Use_nonsilent Non-hypermutated +22 39904088 39904088 TCGA-02-0028 Missense EP300 1 BCM No + EP300|chr22:39904088-39904088 Missense agaggGctggc G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +X 20103288 20103288 TCGA-06-0157 Missense RPS6KA3 1 BCM No - RPS6KA3|chrX:20103288-20103288 Missense tcagcTttttc T A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +X 48935116 48935116 TCGA-06-0157 Missense SYP 1 broad.mit.edu No - SYP|chrX:48935116-48935116 Missense ggttcGtgttt G (CpG) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +X 70237836 70237836 TCGA-02-0083 Missense MLLT7 1 genome.wustl.edu No + MLLT7|chrX:70237836-70237836 Missense cagctCccttt C (TpC) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +X 70504111 70504111 TCGA-06-0213 Missense TAF1 1 BCM No + TAF1|chrX:70504111-70504111 Missense tttggGgctgg G (other) A Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +X 70591395 70591395 TCGA-06-0176 Missense TAF1 1 BCM No + TAF1|chrX:70591395-70591395 Missense agaaaGcctgg G (other) C Somatic Valid Somatic Validated OK Use_nonsilent Non-hypermutated +X 110442569 110442569 TCGA-02-0083 Missense DCX 1 genome.wustl.edu No - DCX|chrX:110442569-110442569 Missense gtctcCcatct C (other) T Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated +X 129111203 129111203 TCGA-02-0114 Missense AIFM1 1 genome.wustl.edu No - AIFM1|chrX:129111203-129111203 Missense aagagGatgaa G (GpA) A Somatic Valid Somatic Validated OK Use_nonsilent Hypermutated From ccbdb59bd67a33818095390d119840cca22fa089 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Tue, 29 Nov 2022 09:55:57 -0800 Subject: [PATCH 009/116] update batch scripts --- test/batch/exit.txt | 2 -- test/batch/multiple_bams.txt | 16 ++++++++-------- 2 files changed, 8 insertions(+), 10 deletions(-) delete mode 100644 test/batch/exit.txt diff --git a/test/batch/exit.txt b/test/batch/exit.txt deleted file mode 100644 index d4c0852a42..0000000000 --- a/test/batch/exit.txt +++ /dev/null @@ -1,2 +0,0 @@ -# Should cause IGV to close -exit diff --git a/test/batch/multiple_bams.txt b/test/batch/multiple_bams.txt index 6c20aafb1b..3c9460e616 100644 --- a/test/batch/multiple_bams.txt +++ b/test/batch/multiple_bams.txt @@ -5,12 +5,12 @@ load gs://genomics-public-data/platinum-genomes/bam/NA12877_S1.bam load gs://genomics-public-data/platinum-genomes/bam/NA12878_S1.bam load gs://genomics-public-data/platinum-genomes/bam/NA12889_S1.bam load gs://genomics-public-data/platinum-genomes/bam/NA12890_S1.bam -#snapshot +snapshot goto myc muc1 -#snapshot myc_myc1.png -# goto tp53 -# zoomin -# zoomin -# zoomin -# squish -#snapshot tp53_squished.png +snapshot myc_myc1.png +goto tp53 +zoomin +zoomin +zoomin +squish +snapshot tp53_squished.png From 59de6e26505045c65c63cfa20ebe722801a86c15 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 7 Dec 2022 20:15:46 -0800 Subject: [PATCH 010/116] Force synchronization of track loading and sorting (be sure load completes before sorting) when processing command links. See issue #1261 --- .../org/broad/igv/batch/CommandExecutor.java | 189 +++++++++--------- src/main/java/org/broad/igv/ui/IGV.java | 11 +- test/web/StaticLinkExamples.html | 10 + 3 files changed, 116 insertions(+), 94 deletions(-) diff --git a/src/main/java/org/broad/igv/batch/CommandExecutor.java b/src/main/java/org/broad/igv/batch/CommandExecutor.java index 032865a8b8..293e7dba21 100755 --- a/src/main/java/org/broad/igv/batch/CommandExecutor.java +++ b/src/main/java/org/broad/igv/batch/CommandExecutor.java @@ -36,7 +36,6 @@ import org.broad.igv.feature.RegionOfInterest; import org.broad.igv.feature.Strand; import org.broad.igv.feature.genome.GenomeManager; -import org.broad.igv.oauth.OAuthUtils; import org.broad.igv.logging.LogManager; import org.broad.igv.logging.Logger; import org.broad.igv.prefs.Constants; @@ -207,7 +206,7 @@ public String execute(String commandLine) { FrameManager.incrementZoom(-1); } else if ("oauth".equals(cmd) || cmd.equalsIgnoreCase("setaccesstoken")) { HttpUtils.getInstance().setAccessToken(param1, param2); - } else if(cmd.equals("clearaccesstokens")) { + } else if (cmd.equals("clearaccesstokens")) { HttpUtils.getInstance().clearAccessTokens(); } else if (cmd.equalsIgnoreCase("sortByAttribute")) { result = sortByAttribute(args); @@ -703,120 +702,132 @@ String loadFiles(final String fileString, String sortTag, boolean dup) { - boolean isDataURL = ParsingUtils.isDataURL(fileString); - - List files = isDataURL ? Arrays.asList(fileString) : breakFileString(fileString); - List indexFiles = isDataURL ? null : breakFileString(indexString); - List coverageFiles = breakFileString(coverageString); - List names = breakFileString(nameString); - List formats = breakFileString(formatString); - if (names != null && names.size() != files.size()) { - return "Error: If file is a comma-separated list, names must also be a comma-separated list of the same length"; - } - if (indexFiles != null && indexFiles.size() != files.size()) { - return "Error: If file is a comma-separated list, index must also be a comma-separated list of the same length"; - } - if (isDataURL && formatString == null) { - return "Error: format must be specified for dataURLs"; - } + boolean currentBatchSetting = Globals.isBatch(); + try { + // Temporarily switch to batch mode to force synchronization of load followed by possible sort + Globals.setBatch(true); + + boolean isDataURL = ParsingUtils.isDataURL(fileString); + + List files = isDataURL ? Arrays.asList(fileString) : breakFileString(fileString); + List indexFiles = isDataURL ? null : breakFileString(indexString); + List coverageFiles = breakFileString(coverageString); + List names = breakFileString(nameString); + List formats = breakFileString(formatString); + if (names != null && names.size() != files.size()) { + return "Error: If file is a comma-separated list, names must also be a comma-separated list of the same length"; + } + if (indexFiles != null && indexFiles.size() != files.size()) { + return "Error: If file is a comma-separated list, index must also be a comma-separated list of the same length"; + } + if (isDataURL && formatString == null) { + return "Error: format must be specified for dataURLs"; + } - // Fix formats -- backward compatibility - if (formats != null) { - for (int i = 0; i < formats.size(); i++) { - String formatOrExt = decodeFileString(formats.get(i)); - String format = formatOrExt.startsWith(".") ? formatOrExt.substring(1) : formatOrExt; - formats.set(i, format); + // Fix formats -- backward compatibility + if (formats != null) { + for (int i = 0; i < formats.size(); i++) { + String formatOrExt = decodeFileString(formats.get(i)); + String format = formatOrExt.startsWith(".") ? formatOrExt.substring(1) : formatOrExt; + formats.set(i, format); + } } - } - List fileLocators = new ArrayList<>(); + List fileLocators = new ArrayList<>(); - if (!SessionReader.isSessionFile(fileString) && merge == false) { - igv.newSession(); - } + if (!SessionReader.isSessionFile(fileString) && merge == false) { + igv.newSession(); + } - // Create set of loaded files - Set loadedFiles = new HashSet<>(); - for (ResourceLocator rl : igv.getDataResourceLocators()) { - loadedFiles.add(rl.getPath()); - } + // Create set of loaded files + Set loadedFiles = new HashSet<>(); + for (ResourceLocator rl : igv.getDataResourceLocators()) { + loadedFiles.add(rl.getPath()); + } - // Loop through files - for (int fi = 0; fi < files.size(); fi++) { + // Loop through files + for (int fi = 0; fi < files.size(); fi++) { - String f = resolveFileReference(files.get(fi)); + String f = resolveFileReference(files.get(fi)); - if (isDataURL && formats == null) { - return "Error: format must be specified for dataURLs"; - } + if (isDataURL && formats == null) { + return "Error: format must be specified for dataURLs"; + } - // Skip already loaded files - if (!dup && loadedFiles.contains(f)) continue; + // Skip already loaded files + if (!dup && loadedFiles.contains(f)) continue; - if (SessionReader.isSessionFile(f)) { - igv.loadSession(f, locus); - } else { + if (SessionReader.isSessionFile(f)) { + igv.loadSession(f, locus); + } else { - ResourceLocator rl = new ResourceLocator(f); + ResourceLocator rl = new ResourceLocator(f); - if (names != null) { - rl.setName(names.get(fi)); - } else if (isDataURL) { - rl.setName("Data"); - } - if (indexFiles != null) { - rl.setIndexPath(indexFiles.get(fi)); - } - if (coverageFiles != null) { - rl.setCoverage(coverageFiles.get(fi)); - } - if (formats != null) { - rl.setFormat(formats.get(fi)); - } + if (names != null) { + rl.setName(names.get(fi)); + } else if (isDataURL) { + rl.setName("Data"); + } + if (indexFiles != null) { + rl.setIndexPath(indexFiles.get(fi)); + } + if (coverageFiles != null) { + rl.setCoverage(coverageFiles.get(fi)); + } + if (formats != null) { + rl.setFormat(formats.get(fi)); + } - if (params != null) { - String trackLine = createTrackLine(params); - rl.setTrackLine(trackLine); - } + if (params != null) { + String trackLine = createTrackLine(params); + rl.setTrackLine(trackLine); + } - if (!isDataURL && rl.isLocal()) { - File file = new File(rl.getPath()); - if (!file.exists()) { - return "Error: " + f + " does not exist."; + if (!isDataURL && rl.isLocal()) { + File file = new File(rl.getPath()); + if (!file.exists()) { + return "Error: " + f + " does not exist."; + } } + fileLocators.add(rl); } - fileLocators.add(rl); } - } - if (fileLocators.size() > 0) { - igv.loadTracks(fileLocators); - } + if (fileLocators.size() > 0) { + igv.loadTracks(fileLocators); + } - if (locus != null && !locus.equals("null")) { - igv.goToLocus(locus); - //If locus is a single base, we sort by base - String[] tokens = locus.split(":", 2); - if (tokens.length == 2) { - try { - int pos = Integer.parseInt(tokens[1].replace(",", "")); - if (pos >= 0 && sort == null) sort = "base"; - } catch (Exception e) { - //pass + + if (locus != null) { + igv.goToLocus(locus); + } + + // Sort alignment tracks + if (igv.getAlignmentTracks().size() > 0) { + + //If locus is a single base, and session has alignment tracks, provide default sort option (base) + if (locus != null && sort == null) { + String[] tokens = locus.split(":", 2); + if (tokens.length == 2 && !tokens[1].contains("-")) { + // Sort by base by default + sort = "base"; + } + } + + if (sort != null) { + final SortOption sortOption = getAlignmentSortOption(sort); + igv.sortAlignmentTracks(sortOption, sortTag, false); } } - } - if (sort != null) { - final SortOption sortOption = getAlignmentSortOption(sort); - igv.sortAlignmentTracks(sortOption, sortTag, false); + return CommandListener.OK; + } finally { + Globals.setBatch(currentBatchSetting); } - - return CommandListener.OK; } private String resolveFileReference(String f) { diff --git a/src/main/java/org/broad/igv/ui/IGV.java b/src/main/java/org/broad/igv/ui/IGV.java index bec6909127..24cb39cb12 100644 --- a/src/main/java/org/broad/igv/ui/IGV.java +++ b/src/main/java/org/broad/igv/ui/IGV.java @@ -1539,15 +1539,16 @@ public List getAllTracks() { } public List getFeatureTracks() { - Iterable featureTracksIter = Iterables.filter(getAllTracks(), FeatureTrack.class); - List featureTracks = Lists.newArrayList(featureTracksIter); - return featureTracks; + return Lists.newArrayList(Iterables.filter(getAllTracks(), FeatureTrack.class)); } public List getDataTracks() { Iterable dataTracksIter = Iterables.filter(getAllTracks(), DataTrack.class); - List dataTracks = Lists.newArrayList(dataTracksIter); - return dataTracks; + return Lists.newArrayList(Iterables.filter(getAllTracks(), DataTrack.class)); + } + + public List getAlignmentTracks() { + return Lists.newArrayList(Iterables.filter(getAllTracks(), AlignmentTrack.class)); } public void clearSelections() { diff --git a/test/web/StaticLinkExamples.html b/test/web/StaticLinkExamples.html index b9bff4e26b..24eb7a87ff 100644 --- a/test/web/StaticLinkExamples.html +++ b/test/web/StaticLinkExamples.html @@ -83,6 +83,16 @@

IGV desktop links

Goto locus 'MYC'

+

Issue 1261

+

+ + Load HG01880 BAM, merge=ask +

+

+ + Load HG01879 BAM, merge=ask +

+

IGV web app links

Date: Thu, 8 Dec 2022 08:36:31 -0800 Subject: [PATCH 011/116] remove unused code --- src/main/java/org/broad/igv/ui/IGV.java | 1 - 1 file changed, 1 deletion(-) diff --git a/src/main/java/org/broad/igv/ui/IGV.java b/src/main/java/org/broad/igv/ui/IGV.java index 24cb39cb12..06f9e7e10b 100644 --- a/src/main/java/org/broad/igv/ui/IGV.java +++ b/src/main/java/org/broad/igv/ui/IGV.java @@ -1543,7 +1543,6 @@ public List getFeatureTracks() { } public List getDataTracks() { - Iterable dataTracksIter = Iterables.filter(getAllTracks(), DataTrack.class); return Lists.newArrayList(Iterables.filter(getAllTracks(), DataTrack.class)); } From 88bf6b8b06698eb738923ebc8a4f2e4c7eb149f1 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 8 Dec 2022 08:42:40 -0800 Subject: [PATCH 012/116] Add NPE protection to alignment sort method. --- src/main/java/org/broad/igv/sam/AlignmentTrack.java | 6 +++++- 1 file changed, 5 insertions(+), 1 deletion(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrack.java b/src/main/java/org/broad/igv/sam/AlignmentTrack.java index d1b799c4b8..42cc23a5b5 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrack.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrack.java @@ -796,7 +796,11 @@ public void sortRows(final SortOption option, final Double location, final Strin for (ReferenceFrame frame : frames) { final double actloc = location != null ? location : frame.getCenter(); final AlignmentInterval interval = getDataManager().getLoadedInterval(frame); - interval.sortRows(option, actloc, tag, invertSort); + if(interval != null) { + interval.sortRows(option, actloc, tag, invertSort); + } else { + log.warn("Attempt to sort alignments prior to loading"); + } } } From a8904b03d6e5ccc76a63f197030a5ecac0d74b8e Mon Sep 17 00:00:00 2001 From: Louis Bergelson Date: Mon, 12 Dec 2022 17:06:46 -0500 Subject: [PATCH 013/116] Fixing issue where non-snp Variants were not properly selectable * There was a bug causing multibase variants to only be selectable when clicking on the first base. They should now be clickable at any position --- .../org/broad/igv/feature/FeatureUtils.java | 47 +++++++------------ .../broad/igv/feature/FeatureUtilsTest.java | 24 ++++++++++ 2 files changed, 41 insertions(+), 30 deletions(-) diff --git a/src/main/java/org/broad/igv/feature/FeatureUtils.java b/src/main/java/org/broad/igv/feature/FeatureUtils.java index 12137b9d69..7e755272b9 100644 --- a/src/main/java/org/broad/igv/feature/FeatureUtils.java +++ b/src/main/java/org/broad/igv/feature/FeatureUtils.java @@ -199,40 +199,27 @@ public static Feature getFeatureCenteredBefore(double position, List features) { - // look for exact match at position: - htsjdk.tribble.Feature f0 = getFeatureAt(position, features); - if (f0 != null) { - return f0; - } - // otherwise look for features on either side and return the closest: - htsjdk.tribble.Feature f1 = getFeatureEndsBefore(position, features); - htsjdk.tribble.Feature f2 = getFeatureStartsAfter(position, features); - - double d1 = f1 == null ? Double.MAX_VALUE : Math.abs(position - f1.getEnd()); - double d2 = f2 == null ? Double.MAX_VALUE : Math.abs(f2.getStart() - position); - - return (d1 < d2 ? f1 : f2); - - } - /** - * Return a feature that encompasses the supplied position. - * - * @param position Query position. - * @param features List of features. - * @return The feature whose start overlaps with position, or null. + * Get the feature which is closest to the given position. + * If multiple features are equidistant it breaks ties by the one that has the closest + * starting position, followed by the shortest feature. */ - private static Feature getFeatureAt(double position, List features) { - int strt = (int) position; - Feature key = new BasicFeature("", strt, strt + 1); - - int r = Collections.binarySearch(features, key, FEATURE_START_COMPARATOR); + public static Feature getFeatureClosest(double position, List features) { + final Comparator closestComparator = Comparator.comparing((Feature f) -> getDistance((int) position, f)) //overlaps or not + .thenComparing(f -> Math.abs(position - f.getStart())) + .thenComparing(Feature::getLengthOnReference); + return Collections.min(features, closestComparator); + } - if (r >= 0) { - return features.get(r); + private static int getDistance(final int position, final Feature f) { + final int end = f.getEnd(); + final int start = f.getStart(); + if(position >= start && position < end){ //within the feature + return 0; + } else if(position >= end){ + return position - end +1; //after the feature } else { - return null; + return start - position; //before the feature } } diff --git a/src/test/java/org/broad/igv/feature/FeatureUtilsTest.java b/src/test/java/org/broad/igv/feature/FeatureUtilsTest.java index 323f9d4f55..f0b419f065 100644 --- a/src/test/java/org/broad/igv/feature/FeatureUtilsTest.java +++ b/src/test/java/org/broad/igv/feature/FeatureUtilsTest.java @@ -45,6 +45,7 @@ import java.io.FileReader; import java.io.IOException; import java.util.ArrayList; +import java.util.Collections; import java.util.List; import static org.junit.Assert.*; @@ -96,6 +97,29 @@ public void testFeatureLookupInSmallVCF() throws IOException { assertEquals("variant closest to 6321740.4 must be found with position=6321740", 6321740, a_6321740.getStart()); } + @Test + public void testGetFeatureClosestWithIndels(){ + Feature snp = new BasicFeature("", 10, 11); + Feature snp2 = new BasicFeature("", 15, 16); + Feature indel = new BasicFeature("", 5, 13); + Feature indel2 = new BasicFeature( "", 25, 31); + List features = List.of(indel, indel2, snp, snp2); // this order is important to make sure tiebreakers are woorking + assertEquals(FeatureUtils.getFeatureClosest(1, features), indel); + assertEquals(FeatureUtils.getFeatureClosest(4, features), indel); + assertEquals(FeatureUtils.getFeatureClosest(5, features), indel); + assertEquals(FeatureUtils.getFeatureClosest(9, features), indel); + assertEquals("since indel and snp overlap at this point it should pick the snp", FeatureUtils.getFeatureClosest( 10, features), snp); + assertEquals(FeatureUtils.getFeatureClosest(11, features), indel); + assertEquals(FeatureUtils.getFeatureClosest(12, features), indel); + assertEquals(FeatureUtils.getFeatureClosest(13, features), indel); + assertEquals(FeatureUtils.getFeatureClosest(14, features), snp2); + assertEquals(FeatureUtils.getFeatureClosest(15, features), snp2); + assertEquals(FeatureUtils.getFeatureClosest(16, features), snp2); + assertEquals(FeatureUtils.getFeatureClosest(20, features), snp2); + assertEquals(FeatureUtils.getFeatureClosest(21, features), indel2); + assertEquals(FeatureUtils.getFeatureClosest(100, features), indel2); + } + /** * Test of getAllFeaturesAt method, of class FeatureUtils. *

From ce7ec07e9b2f35120d8db7f74a7484a38b1cae8c Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 14 Dec 2022 09:04:19 -0800 Subject: [PATCH 014/116] igv dataset parser tweak to handle empty lines --- src/main/java/org/broad/igv/data/IGVDatasetParser.java | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/src/main/java/org/broad/igv/data/IGVDatasetParser.java b/src/main/java/org/broad/igv/data/IGVDatasetParser.java index 4215b39276..30bcfc363b 100644 --- a/src/main/java/org/broad/igv/data/IGVDatasetParser.java +++ b/src/main/java/org/broad/igv/data/IGVDatasetParser.java @@ -173,7 +173,7 @@ public List scan(IGVDataset dataset) { } // Parse comments and directives, if any - nextLine = reader.readLine(); + nextLine = reader.readLine().trim(); while (nextLine.startsWith("#") || (nextLine.trim().length() == 0)) { headerRows++; From f36def74cade1cbdde5c184c91454b846c5afe7a Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 14 Dec 2022 13:35:21 -0800 Subject: [PATCH 015/116] Adjust exeperiment type test for 3rd gen --- src/main/java/org/broad/igv/sam/ReadStats.java | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/src/main/java/org/broad/igv/sam/ReadStats.java b/src/main/java/org/broad/igv/sam/ReadStats.java index 4485d1bd46..2090095980 100644 --- a/src/main/java/org/broad/igv/sam/ReadStats.java +++ b/src/main/java/org/broad/igv/sam/ReadStats.java @@ -135,7 +135,7 @@ private double[] downsample(DoubleArrayList list, int size) { public AlignmentTrack.ExperimentType inferType() { compute(); if (readCount < 100) return null; // Not enough reads - if ((readLengthStdDev > 100 || medianReadLength > 1000) && averageCigarLength > 100) { + if ((readLengthStdDev > 100 || medianReadLength > 1000) && averageCigarLength > 10) { // Cigar length to filter consensus reads return AlignmentTrack.ExperimentType.THIRD_GEN; } else if (medianRefToReadRatio > 5 || fracReadsWithNs > 0.01) { return AlignmentTrack.ExperimentType.RNA; From e6afabcd0b3d7486fcbadf1971c3bec244721e45 Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Thu, 15 Dec 2022 20:46:07 -0800 Subject: [PATCH 016/116] Ctsa ipd color2 (#1270) * Add SMRT kinetics color-by options - IGV now parses SMRT kinetics frame count tags in both 8-bit compressed format, and 16-bit uncompressed format. This is enabled by a new user preference option under the "3rd gen" tab. Co-authored-by: Chris Saunders Co-authored-by: Alexandr Chernov --- src/main/java/module-info.java | 1 + .../java/org/broad/igv/prefs/Constants.java | 2 + .../java/org/broad/igv/sam/Alignment.java | 8 ++ .../org/broad/igv/sam/AlignmentRenderer.java | 22 ++- .../org/broad/igv/sam/AlignmentTrack.java | 23 +++- .../org/broad/igv/sam/AlignmentTrackMenu.java | 16 +++ .../java/org/broad/igv/sam/ReadStats.java | 2 +- .../java/org/broad/igv/sam/SAMAlignment.java | 91 ++++++++++-- .../broad/igv/sam/SMRTKineticsDecoder.java | 40 ++++++ .../org/broad/igv/sam/smrt/SMRTKinetics.java | 129 ++++++++++++++++++ .../igv/sam/smrt/SMRTKineticsDecoder.java | 43 ++++++ .../igv/sam/smrt/SMRTKineticsRenderer.java | 109 +++++++++++++++ .../org/broad/igv/prefs/preferences.tab | 3 + .../igv/sam/smrt/SMRTKineticsDecoderTest.java | 48 +++++++ 14 files changed, 520 insertions(+), 17 deletions(-) create mode 100644 src/main/java/org/broad/igv/sam/SMRTKineticsDecoder.java create mode 100644 src/main/java/org/broad/igv/sam/smrt/SMRTKinetics.java create mode 100644 src/main/java/org/broad/igv/sam/smrt/SMRTKineticsDecoder.java create mode 100644 src/main/java/org/broad/igv/sam/smrt/SMRTKineticsRenderer.java create mode 100644 src/test/java/org/broad/igv/sam/smrt/SMRTKineticsDecoderTest.java diff --git a/src/main/java/module-info.java b/src/main/java/module-info.java index ca3128966c..15bb2a73f2 100644 --- a/src/main/java/module-info.java +++ b/src/main/java/module-info.java @@ -5,6 +5,7 @@ exports org.broad.igv.jbrowse; exports org.broad.igv.logging; exports org.broad.igv.util.liftover; + exports org.broad.igv.sam.smrt; requires com.google.common; requires commons.math3; diff --git a/src/main/java/org/broad/igv/prefs/Constants.java b/src/main/java/org/broad/igv/prefs/Constants.java index bdc8cad8cb..ce0f93e133 100644 --- a/src/main/java/org/broad/igv/prefs/Constants.java +++ b/src/main/java/org/broad/igv/prefs/Constants.java @@ -175,6 +175,8 @@ private Constants() { public static final String SAM_DISPLAY_PAIRED = "SAM.DISPLAY_PAIRED"; public static final String KNOWN_SNPS = "KNOWN_SNPS_FILE"; + public static final String SMRT_KINETICS_SHOW_OPTIONS = "SMRT_KINETICS.SHOW_OPTIONS"; + // Sequence track settings public static final String SEQUENCE_TRANSLATION_STRAND = "SEQUENCE_TRANSLATION_STRAND"; public static final String SHOW_SEQUENCE_TRANSLATION = "SHOW_SEQUENCE_TRANSLATION"; diff --git a/src/main/java/org/broad/igv/sam/Alignment.java b/src/main/java/org/broad/igv/sam/Alignment.java index bef2e3423b..872cac0577 100644 --- a/src/main/java/org/broad/igv/sam/Alignment.java +++ b/src/main/java/org/broad/igv/sam/Alignment.java @@ -33,6 +33,7 @@ import org.broad.igv.feature.Strand; import org.broad.igv.sam.mods.BaseModificationUtils; import org.broad.igv.sam.mods.BaseModificationSet; +import org.broad.igv.sam.smrt.SMRTKinetics; import org.broad.igv.track.WindowFunction; import java.awt.*; @@ -74,6 +75,10 @@ default String getReadSequence() { int getInferredInsertSize(); + default int getLeadingHardClipLength() { + return 0; + } + int getMappingQuality(); ReadMate getMate(); @@ -167,6 +172,9 @@ default void setHaplotypeName(String hap) {} default List getBaseModificationSets() { return null;} + default SMRTKinetics getSmrtKinetics() { return null;} + + default String getAlignmentValueString(double position, int mouseX, AlignmentTrack.RenderOptions renderOptions) { return getValueString(position, mouseX, (WindowFunction) null); } diff --git a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java index 27057a6eea..e9743bdacc 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java +++ b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java @@ -39,6 +39,7 @@ import org.broad.igv.sam.AlignmentTrack.ColorOption; import org.broad.igv.sam.BisulfiteBaseInfo.DisplayStatus; import org.broad.igv.sam.mods.BaseModificationRenderer; +import org.broad.igv.sam.smrt.SMRTKineticsRenderer; import org.broad.igv.track.RenderContext; import org.broad.igv.track.Track; import org.broad.igv.ui.FontManager; @@ -315,9 +316,11 @@ public void renderAlignments(List alignments, double pixelWidth = pixelEnd - pixelStart; Color alignmentColor = getAlignmentColor(alignment, track); final boolean leaveMargin = (this.track.getDisplayMode() != Track.DisplayMode.SQUISHED); + final ColorOption colorOption = renderOptions.getColorOption(); if ((pixelWidth < 2) && - !((AlignmentTrack.isBisulfiteColorType(renderOptions.getColorOption()) || - renderOptions.getColorOption().isBaseMod()) && + !((AlignmentTrack.isBisulfiteColorType(colorOption) || + colorOption.isBaseMod() || + colorOption.isSMRTKinetics()) && (pixelWidth >= 1))) { // Optimization for really zoomed out views. If this alignment occupies screen space already taken, // and it is the default color, skip drawing. @@ -797,7 +800,8 @@ private void drawAlignment( Color color = null; if (bisulfiteMode) { color = bisinfo.getDisplayColor(idx); - } else if (colorOption.isBaseMod()) { + } else if (colorOption.isBaseMod() || + colorOption.isSMRTKinetics()) { color = Color.GRAY; } else { color = nucleotideColors.get(c); @@ -832,6 +836,11 @@ private void drawAlignment( BaseModificationRenderer.drawModifications(alignment, bpStart, locScale, rowRect, context.getGraphics(), colorOption); } + // Kinetic data + if (colorOption.isSMRTKinetics()) { + SMRTKineticsRenderer.drawSmrtKinetics(alignment, bpStart, locScale, rowRect, context.getGraphics(), colorOption); + } + // DRAW Insertions AlignmentBlock[] insertions = alignment.getInsertions(); if (insertions != null) { @@ -945,6 +954,7 @@ private Color getShadedColor(byte qual, Color foregroundColor, Color backgroundC return color; } + private void drawLargeIndelLabel(Graphics2D g, boolean isInsertion, String labelText, int pxCenter, int pxTop, int pxH, int pxWmax, int translateX, AlignmentBlock insertionBlock) { @@ -1176,6 +1186,12 @@ private Color getAlignmentColor(Alignment alignment, AlignmentTrack track) { case BASE_MODIFICATION: case BASE_MODIFICATION_5MC: case BASE_MODIFICATION_C: + case SMRT_SUBREAD_IPD: + case SMRT_SUBREAD_PW: + case SMRT_CCS_FWD_IPD: + case SMRT_CCS_FWD_PW: + case SMRT_CCS_REV_IPD: + case SMRT_CCS_REV_PW: // Just a simple forward/reverse strand color scheme that won't clash with the // methylation rectangles. c = (alignment.getFirstOfPairStrand() == Strand.POSITIVE) ? bisulfiteColorFw1 : bisulfiteColorRev1; diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrack.java b/src/main/java/org/broad/igv/sam/AlignmentTrack.java index 42cc23a5b5..117d9c0511 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrack.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrack.java @@ -100,14 +100,35 @@ public enum ColorOption { YC_TAG, BASE_MODIFICATION, BASE_MODIFICATION_5MC, - BASE_MODIFICATION_C; + BASE_MODIFICATION_C, + SMRT_SUBREAD_IPD, + SMRT_SUBREAD_PW, + SMRT_CCS_FWD_IPD, + SMRT_CCS_FWD_PW, + SMRT_CCS_REV_IPD, + SMRT_CCS_REV_PW; public boolean isBaseMod() { return this == BASE_MODIFICATION || this == BASE_MODIFICATION_5MC || this == BASE_MODIFICATION_C; } + + public boolean isSMRTKinetics() { + switch (this) { + case SMRT_SUBREAD_IPD: + case SMRT_SUBREAD_PW: + case SMRT_CCS_FWD_IPD: + case SMRT_CCS_REV_IPD: + case SMRT_CCS_FWD_PW: + case SMRT_CCS_REV_PW: + return true; + default: + return false; + } + } } + public enum ShadeAlignmentsOption { NONE("none"), MAPPING_QUALITY_HIGH("mapping quality high"), diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index 59f3105107..13969b1dcf 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -578,6 +578,22 @@ void addColorByMenuItem() { group.add(mi); } + if (alignmentTrack.getPreferences().getAsBoolean(SMRT_KINETICS_SHOW_OPTIONS)) { + // Show additional options to help visualize SMRT kinetics data + mappings.clear(); + mappings.put("SMRT subread IPD", AlignmentTrack.ColorOption.SMRT_SUBREAD_IPD); + mappings.put("SMRT subread PW", AlignmentTrack.ColorOption.SMRT_SUBREAD_PW); + mappings.put("SMRT CCS fwd-strand aligned IPD", AlignmentTrack.ColorOption.SMRT_CCS_FWD_IPD); + mappings.put("SMRT CCS fwd-strand aligned PW", AlignmentTrack.ColorOption.SMRT_CCS_FWD_PW); + mappings.put("SMRT CCS rev-strand aligned IPD", AlignmentTrack.ColorOption.SMRT_CCS_REV_IPD); + mappings.put("SMRT CCS rev-strand aligned PW",AlignmentTrack.ColorOption.SMRT_CCS_REV_PW); + colorMenu.addSeparator(); + for (Map.Entry el : mappings.entrySet()) { + JRadioButtonMenuItem mi = getColorMenuItem(el.getKey(), el.getValue()); + colorMenu.add(mi); + group.add(mi); + } + } add(colorMenu); diff --git a/src/main/java/org/broad/igv/sam/ReadStats.java b/src/main/java/org/broad/igv/sam/ReadStats.java index 2090095980..cf2981c509 100644 --- a/src/main/java/org/broad/igv/sam/ReadStats.java +++ b/src/main/java/org/broad/igv/sam/ReadStats.java @@ -134,7 +134,7 @@ private double[] downsample(DoubleArrayList list, int size) { public AlignmentTrack.ExperimentType inferType() { compute(); - if (readCount < 100) return null; // Not enough reads + if (readCount < 20) return null; // Not enough reads if ((readLengthStdDev > 100 || medianReadLength > 1000) && averageCigarLength > 10) { // Cigar length to filter consensus reads return AlignmentTrack.ExperimentType.THIRD_GEN; } else if (medianRefToReadRatio > 5 || fracReadsWithNs > 0.01) { diff --git a/src/main/java/org/broad/igv/sam/SAMAlignment.java b/src/main/java/org/broad/igv/sam/SAMAlignment.java index 41ee8a6784..884fd4ce1e 100644 --- a/src/main/java/org/broad/igv/sam/SAMAlignment.java +++ b/src/main/java/org/broad/igv/sam/SAMAlignment.java @@ -41,6 +41,8 @@ import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.sam.mods.BaseModificationUtils; import org.broad.igv.sam.mods.BaseModificationSet; +import org.broad.igv.sam.smrt.SMRTKinetics; +import org.broad.igv.sam.smrt.SMRTKineticsDecoder; import org.broad.igv.ui.color.ColorUtilities; import java.awt.*; @@ -103,16 +105,16 @@ public class SAMAlignment implements Alignment { public AlignmentBlockImpl[] insertions; List gaps; char[] gapTypes; + protected String mateSequence = null; + protected String pairOrientation = ""; + private Strand firstOfPairStrand; + private Strand secondOfPairStrand; /** * Map of position -> base modification */ private List baseModificationSets; - - protected String mateSequence = null; - protected String pairOrientation = ""; - private Strand firstOfPairStrand; - private Strand secondOfPairStrand; + private SMRTKinetics smrtKinetics; public SAMAlignment(SAMRecord record) { @@ -157,7 +159,6 @@ public SAMAlignment(SAMRecord record) { setPairOrientation(); setPairStrands(); createAlignmentBlocks(); - } public SAMRecord getRecord() { @@ -340,6 +341,13 @@ public List getBaseModificationSets() { return baseModificationSets; } + public SMRTKinetics getSmrtKinetics() { + if (smrtKinetics == null) { + smrtKinetics = new SMRTKinetics(this); + } + return smrtKinetics; + } + /** * Set pair strands. Used for strand specific libraries to recover strand of * originating fragment. @@ -379,6 +387,26 @@ private void setPairStrands() { } } + /** + * Return the number of bases hard-clipped from the leading-edge (leftmost view in IGV) of the alignment + */ + public int getLeadingHardClipLength() { + int clipLength = 0; + + String cigarString = record.getCigarString(); + if (!cigarString.equals("*")) { + java.util.List operators = buildOperators(cigarString); + for (CigarOperator operator : operators) { + if (operator.operator == HARD_CLIP) { + clipLength += operator.nBases; + } else { + break; + } + } + } + return clipLength; + } + /** * Create the alignment blocks from the read bases and alignment information in the CIGAR * string. The CIGAR string encodes insertions, deletions, skipped regions, and padding. @@ -579,6 +607,14 @@ public String getClipboardString(double location, int mouseX) { return getAlignmentValueString(location, mouseX, (AlignmentTrack.RenderOptions) null); } + private Integer positionToReadIndex(double position) { + for (AlignmentBlock block : this.alignmentBlocks) { + if (block.contains((int) position)) { + return (int) (position - block.getStart()) + block.getBasesOffset(); + } + } + return null; + } public String getAlignmentValueString(double position, int mouseX, AlignmentTrack.RenderOptions renderOptions) { @@ -614,12 +650,13 @@ public String getAlignmentValueString(double position, int mouseX, AlignmentTrac } } - // Check base modifications - if (renderOptions != null && - (renderOptions.getColorOption().isBaseMod())) { - for (AlignmentBlock block : this.alignmentBlocks) { - if (block.contains((int) position)) { - int p = (int) (position - block.getStart()) + block.getBasesOffset(); + // Check base modifications & kinetics + if (renderOptions != null) { + final AlignmentTrack.ColorOption colorOption = renderOptions.getColorOption(); + if (colorOption.isBaseMod()) { + Integer readIndex = positionToReadIndex(position); + if (readIndex != null) { + int p = readIndex; if (baseModificationSets != null) { String modString = ""; for (BaseModificationSet bmSet : baseModificationSets) { @@ -635,6 +672,36 @@ public String getAlignmentValueString(double position, int mouseX, AlignmentTrac } } } + } else if (colorOption.isSMRTKinetics()) { + Integer readIndex = positionToReadIndex(position); + SMRTKinetics sk = getSmrtKinetics(); + if (readIndex != null) { + if (colorOption == AlignmentTrack.ColorOption.SMRT_SUBREAD_IPD) { + short[] ipdVals = sk.getSmrtSubreadIpd(); + if (ipdVals != null) { + return "Subread IPD: " + Short.toUnsignedInt(ipdVals[readIndex]) + " Frames"; + } + } else if (colorOption == AlignmentTrack.ColorOption.SMRT_SUBREAD_PW) { + short[] pwVals = sk.getSmrtSubreadPw(); + if (pwVals != null) { + return "Subread PW: " + Short.toUnsignedInt(pwVals[readIndex]) + " Frames"; + } + } else if (colorOption == AlignmentTrack.ColorOption.SMRT_CCS_FWD_IPD || colorOption == AlignmentTrack.ColorOption.SMRT_CCS_REV_IPD) { + final boolean isForwardStrand = (colorOption == AlignmentTrack.ColorOption.SMRT_CCS_FWD_IPD); + short[] ipdVals = sk.getSmrtCcsIpd(isForwardStrand); + if (ipdVals != null) { + final String strand = (isForwardStrand ? "fwd" : "rev"); + return "CCS " + strand + "-strand aligned IPD: " + Short.toUnsignedInt(ipdVals[readIndex]) + " Frames"; + } + } else { + final boolean isForwardStrand = (colorOption == AlignmentTrack.ColorOption.SMRT_CCS_FWD_PW); + short[] pwVals = sk.getSmrtCcsPw(isForwardStrand); + if (pwVals != null) { + final String strand = (isForwardStrand ? "fwd" : "rev"); + return "CCS " + strand + "-strand aligned PW: " + Short.toUnsignedInt(pwVals[readIndex]) + " Frames"; + } + } + } } } diff --git a/src/main/java/org/broad/igv/sam/SMRTKineticsDecoder.java b/src/main/java/org/broad/igv/sam/SMRTKineticsDecoder.java new file mode 100644 index 0000000000..566913c204 --- /dev/null +++ b/src/main/java/org/broad/igv/sam/SMRTKineticsDecoder.java @@ -0,0 +1,40 @@ +package org.broad.igv.sam; + +/// Decode SMRT kinetic values from uint8 compressed code to an approximation of the +/// original frame count. +/// +public class SMRTKineticsDecoder { + + private static final int base = 1 << 6; + + private final short[] frameCounts; + + public SMRTKineticsDecoder() { + frameCounts = new short[256]; + for (int i = 0; i < 256; ++i) { + frameCounts[i] = decodeFrameCount((byte) i); + } + } + + /// Convert compressed byte to frame count from cached LUT + public short lookupFrameCount(byte val) { + return frameCounts[val & 0xFF]; + } + + /// Compute frame count from compressed byte + /// + /// 8-bit compressed V1 codec: + /// + /// xxmm'mmmm + /// + /// where + /// * 'x' represents the exponent (2 MSBs) + /// * 'm' represents the mantissa (6 LSBs) + /// + private static short decodeFrameCount(byte val) { + int mantissa = val & 0b0011_1111; + int exponent = (val & 0b1100_0000) >> 6; + int exponentVal = 1 << exponent; + return (short) (base * (exponentVal - 1) + exponentVal * mantissa); + } +} diff --git a/src/main/java/org/broad/igv/sam/smrt/SMRTKinetics.java b/src/main/java/org/broad/igv/sam/smrt/SMRTKinetics.java new file mode 100644 index 0000000000..d3f8bf5984 --- /dev/null +++ b/src/main/java/org/broad/igv/sam/smrt/SMRTKinetics.java @@ -0,0 +1,129 @@ +package org.broad.igv.sam.smrt; + +import org.broad.igv.sam.Alignment; +import org.broad.igv.sam.SAMAlignment; + +public class SMRTKinetics { + + private Alignment alignment; + private short[] smrtSubreadIpd; + private short[] smrtSubreadPw; + private short[] smrtCcsIpd; + private short[] smrtCcsPw; + + private short[] smrtSubreadIpdVals; + private short[] smrtSubreadPwVals; + private short[] smrtCcsFwdIpdVals; + private short[] smrtCcsFwdPwVals; + private short[] smrtCcsRevIpdVals; + private short[] smrtCcsRevPwVals; + + public SMRTKinetics(Alignment alignment) { + this.alignment = alignment; + } + + + /** + * Given the compressed byte code array of CCS kinetic values from the SAM aux field do the following: + * - Initialize frame count array, decode compressed kinetic byte codes into frame counts and copy these into it + * - Reverse frame count sequence if reverseSequence is true + * - If the read has a leading hard-clip, remove all frame counts from the hard-clipped region + * + * Returns the processed frame count array + */ + private short[] parseSmrtKineticByteCodes(byte[] smrtKineticByteCodes, boolean reverseSequence) { + if (smrtKineticByteCodes.length == 0) { + return null; + } + + int hardClipLength = alignment. getLeadingHardClipLength(); + int kineticValLength = smrtKineticByteCodes.length - hardClipLength; + assert(kineticValLength > 0); + short[] ccsKineticVals = new short[kineticValLength]; + + int byteCodeIndex = 0; + for (byte ccsKineticByteCode : smrtKineticByteCodes) { + int valIndex = byteCodeIndex; + byteCodeIndex++; + if (reverseSequence) { + valIndex = smrtKineticByteCodes.length - (valIndex+1); + } + if (valIndex < hardClipLength) continue; + valIndex -= hardClipLength; + ccsKineticVals[valIndex] = SMRTKineticsDecoder.lookupFrameCount(ccsKineticByteCode); + } + return ccsKineticVals; + } + + /** + * Given the original uint16 CCS kinetic frame count array from the SAM aux field do the following: + * - Initialize the processed frame count array and copy values into it + * - Reverse frame count sequence if reverseSequence is true + * - If the read has a leading hard-clip, remove all frame counts from the hard-clipped region + *

+ * Returns the processed frame count array + */ + private short[] parseAuxSmrtKineticFrameCounts(short[] auxSmrtKineticFrameCounts, boolean reverseSequence) { + if (auxSmrtKineticFrameCounts.length == 0) { + return null; + } + + int hardClipLength = alignment.getLeadingHardClipLength(); + int kineticValLength = auxSmrtKineticFrameCounts.length - hardClipLength; + assert(kineticValLength > 0); + short[] ccsKineticVals = new short[kineticValLength]; + + int auxIndex = 0; + for (short ccsKineticFrameCount : auxSmrtKineticFrameCounts) { + int valIndex = auxIndex; + auxIndex++; + if (reverseSequence) { + valIndex = auxSmrtKineticFrameCounts.length - (valIndex+1); + } + if (valIndex < hardClipLength) continue; + valIndex -= hardClipLength; + ccsKineticVals[valIndex] = ccsKineticFrameCount; + } + return ccsKineticVals; + } + + private short[] getSmrtKineticsVals(short[] smrtKineticVals, String tag, boolean bytesReversedInBam) { + if (smrtKineticVals == null && alignment.getAttribute(tag) != null) { + final boolean reverseSequence = (bytesReversedInBam ^ alignment.isNegativeStrand()); + Object tagValue = alignment.getAttribute(tag); + if (tagValue instanceof byte[]) { + smrtKineticVals = parseSmrtKineticByteCodes((byte[]) (tagValue), reverseSequence); + } else if (tagValue instanceof short[]) { + smrtKineticVals = parseAuxSmrtKineticFrameCounts((short[]) (tagValue), reverseSequence); + } else { + throw new RuntimeException("Unexpected format in SMRT kinetic aux tag '" + tag + "'"); + } + } + return smrtKineticVals; + } + + + public short[] getSmrtSubreadIpd() { + return getSmrtKineticsVals(smrtSubreadIpdVals, "ip", false); + } + + public short[] getSmrtSubreadPw() { + return getSmrtKineticsVals(smrtSubreadPwVals, "pw", false); + } + + public short[] getSmrtCcsIpd(boolean isForwardStrand) { + if (isForwardStrand ^ alignment.isNegativeStrand()) { + return getSmrtKineticsVals(smrtCcsFwdIpdVals, "fi", false); + } else { + return getSmrtKineticsVals(smrtCcsRevIpdVals, "ri", true); + } + } + + public short[] getSmrtCcsPw(boolean isForwardStrand) { + if (isForwardStrand ^ alignment.isNegativeStrand()) { + return getSmrtKineticsVals(smrtCcsFwdPwVals, "fp", false); + } else { + return getSmrtKineticsVals(smrtCcsRevPwVals, "rp", true); + } + } +} diff --git a/src/main/java/org/broad/igv/sam/smrt/SMRTKineticsDecoder.java b/src/main/java/org/broad/igv/sam/smrt/SMRTKineticsDecoder.java new file mode 100644 index 0000000000..518ff42e54 --- /dev/null +++ b/src/main/java/org/broad/igv/sam/smrt/SMRTKineticsDecoder.java @@ -0,0 +1,43 @@ +package org.broad.igv.sam.smrt; + +/// Decode SMRT kinetic values from uint8 compressed code to an approximation of the +/// original frame count. +/// +public class SMRTKineticsDecoder { + + private static final int base = 1 << 6; + + private static short[] frameCounts; + + private SMRTKineticsDecoder() { + + } + + /// Convert compressed byte to frame count from cached LUT + public static short lookupFrameCount(byte val) { + if(frameCounts == null) { + frameCounts = new short[256]; + for (int i = 0; i < 256; ++i) { + frameCounts[i] = decodeFrameCount((byte) i); + } + } + return frameCounts[val & 0xFF]; + } + + /// Compute frame count from compressed byte + /// + /// 8-bit compressed V1 codec: + /// + /// xxmm'mmmm + /// + /// where + /// * 'x' represents the exponent (2 MSBs) + /// * 'm' represents the mantissa (6 LSBs) + /// + private static short decodeFrameCount(byte val) { + int mantissa = val & 0b0011_1111; + int exponent = (val & 0b1100_0000) >> 6; + int exponentVal = 1 << exponent; + return (short) (base * (exponentVal - 1) + exponentVal * mantissa); + } +} diff --git a/src/main/java/org/broad/igv/sam/smrt/SMRTKineticsRenderer.java b/src/main/java/org/broad/igv/sam/smrt/SMRTKineticsRenderer.java new file mode 100644 index 0000000000..087c5b2ec5 --- /dev/null +++ b/src/main/java/org/broad/igv/sam/smrt/SMRTKineticsRenderer.java @@ -0,0 +1,109 @@ +package org.broad.igv.sam.smrt; + +import org.broad.igv.sam.*; + +import java.awt.*; + +public class SMRTKineticsRenderer { + public static void drawSmrtKinetics(Alignment alignment, double bpStart, double locScale, Rectangle rowRect, Graphics g, AlignmentTrack.ColorOption colorOption) { + int dX; + short[] smrtFrameCounts; + SMRTKinetics smrtKinetics = alignment.getSmrtKinetics(); + if (AlignmentTrack.ColorOption.SMRT_SUBREAD_IPD == colorOption) { + smrtFrameCounts = smrtKinetics.getSmrtSubreadIpd(); + } else if (AlignmentTrack.ColorOption.SMRT_SUBREAD_PW == colorOption) { + smrtFrameCounts = smrtKinetics.getSmrtSubreadPw(); + } else if (AlignmentTrack.ColorOption.SMRT_CCS_FWD_IPD == colorOption || AlignmentTrack.ColorOption.SMRT_CCS_REV_IPD == colorOption) { + final boolean isForwardStrand = (AlignmentTrack.ColorOption.SMRT_CCS_FWD_IPD == colorOption); + smrtFrameCounts = smrtKinetics.getSmrtCcsIpd(isForwardStrand); + } else { + final boolean isForwardStrand = (AlignmentTrack.ColorOption.SMRT_CCS_FWD_PW == colorOption); + smrtFrameCounts = smrtKinetics.getSmrtCcsPw(isForwardStrand); + } + + if (smrtFrameCounts != null) { + // Compute bounds + int pY = (int) rowRect.getY(); + int dY = (int) rowRect.getHeight(); + dX = (int) Math.max(1, (1.0 / locScale)); + + for (AlignmentBlock block : alignment.getAlignmentBlocks()) { + ByteSubarray bases = block.getBases(); + final int startOffset = bases.startOffset; + final int stopOffset = startOffset + bases.length; + for (int i = startOffset; i < stopOffset; i++) { + g.setColor(getSmrtFrameCountColor(smrtFrameCounts[i])); + + int blockIdx = i - block.getBases().startOffset; + int pX = (int) ((block.getStart() + blockIdx - bpStart) / locScale); + + // Don't draw out of clipping rect + if (pX > rowRect.getMaxX()) { + break; + } else if (pX + dX < rowRect.getX()) { + continue; + } + + // Expand narrow width to make more visible + if (dX < 3) { + dX = 3; + pX--; + } + g.fillRect(pX, pY, dX, Math.max(1, dY - 2)); + } + } + } + } + + /** + * Set base color for SMRT sequencing frame count + *

+ * This color scheme uses multiple transitions to help visually distinguish a large range of time intervals. + *

+ * Color scheme: + * The color starts out as transparent at a frame count of 0. + * - Color transition 1 goes from transparent to opaque red + * - Color transition 2 goes from red to yellow + * - Color transition 3 goes from yellow to cyan + *

+ * Transition 3 will mostly be visible when uncompressed frame counts are used. It helps to visually distinguish + * exceptional polymerase stalling events. + */ + private static Color getSmrtFrameCountColor(short shortFrameCount) { + final int transition1MaxFrameCount = 100; + final int transition2MaxFrameCount = 600; + final int transition3MaxFrameCount = 6000; + final Color color1 = Color.red; + final Color color2 = Color.yellow; + final Color color3 = Color.cyan; + + final int frameCount = Short.toUnsignedInt(shortFrameCount); + final int alpha = Math.min(255, (frameCount * 255) / transition1MaxFrameCount); + Color blendedColor; + if (frameCount <= transition1MaxFrameCount) { + blendedColor = color1; + } else if (frameCount <= transition2MaxFrameCount) { + float color2Fraction = (frameCount - transition1MaxFrameCount) / ((float) (transition2MaxFrameCount - transition1MaxFrameCount)); + blendedColor = blendColors(color1, color2, color2Fraction); + } else if (frameCount <= transition3MaxFrameCount) { + float color3Fraction = (frameCount - transition2MaxFrameCount) / ((float) (transition3MaxFrameCount - transition2MaxFrameCount)); + blendedColor = blendColors(color2, color3, color3Fraction); + } else { + blendedColor = color3; + } + return new Color(blendedColor.getRed(), blendedColor.getGreen(), blendedColor.getBlue(), alpha); + } + + + private static int blendColorValue(int v1, int v2, float v2Frac) { + return Math.min(255, (int) (v1 + (v2 - v1) * v2Frac)); + } + + private static Color blendColors(Color c1, Color c2, float c2Frac) { + assert (c2Frac >= 0.0 && c2Frac <= 1.0); + return new Color(blendColorValue(c1.getRed(), c2.getRed(), c2Frac), + blendColorValue(c1.getGreen(), c2.getGreen(), c2Frac), + blendColorValue(c1.getBlue(), c2.getBlue(), c2Frac)); + } + +} diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index cc4b8df723..9129618993 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -191,6 +191,9 @@ SAM.SHOW_INSERTION_MARKERS Show insertion markers boolean TRUE SAM.LINK_READS Link alignments by tag boolean FALSE SAM.LINK_TAG Linking tag string READNAME +##SMRT Kinetics +SMRT_KINETICS.SHOW_OPTIONS Show visibility options for SMRT kinetics data boolean FALSE + #Proxy PROXY.DISABLE_CHECK Disable check for system proxy boolean FALSE PROXY.USE Use proxy boolean FALSE diff --git a/src/test/java/org/broad/igv/sam/smrt/SMRTKineticsDecoderTest.java b/src/test/java/org/broad/igv/sam/smrt/SMRTKineticsDecoderTest.java new file mode 100644 index 0000000000..f258dd0b0c --- /dev/null +++ b/src/test/java/org/broad/igv/sam/smrt/SMRTKineticsDecoderTest.java @@ -0,0 +1,48 @@ +/* + * The MIT License (MIT) + * + * Copyright (c) 2007-2015 Broad Institute + * + * Permission is hereby granted, free of charge, to any person obtaining a copy + * of this software and associated documentation files (the "Software"), to deal + * in the Software without restriction, including without limitation the rights + * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell + * copies of the Software, and to permit persons to whom the Software is + * furnished to do so, subject to the following conditions: + * + * The above copyright notice and this permission notice shall be included in + * all copies or substantial portions of the Software. + * + * + * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR + * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, + * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE + * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER + * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, + * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN + * THE SOFTWARE. + */ + +package org.broad.igv.sam.smrt; + +import org.junit.Test; + +import static org.junit.Assert.assertEquals; + +public class SMRTKineticsDecoderTest { + + public SMRTKineticsDecoderTest() { + } + + @Test + public void testDecoder() throws Exception { + + short v0 = SMRTKineticsDecoder.lookupFrameCount((byte) 0); + short v128 = SMRTKineticsDecoder.lookupFrameCount((byte) 128); + short v255 = SMRTKineticsDecoder.lookupFrameCount((byte) 255); + + assertEquals(0, v0); + assertEquals(192, v128); + assertEquals(952, v255); + } +} \ No newline at end of file From f5ae7f471ffe2f831c7a48e6362e41e022454127 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Fri, 16 Dec 2022 09:26:37 -0800 Subject: [PATCH 017/116] Allow merging bam files with different chr name conventions ("1" vs "chr1") --- .../igv/sam/reader/MergedAlignmentReader.java | 66 ++++++++++--------- 1 file changed, 35 insertions(+), 31 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/reader/MergedAlignmentReader.java b/src/main/java/org/broad/igv/sam/reader/MergedAlignmentReader.java index 10e94799ef..f1794efcd7 100644 --- a/src/main/java/org/broad/igv/sam/reader/MergedAlignmentReader.java +++ b/src/main/java/org/broad/igv/sam/reader/MergedAlignmentReader.java @@ -49,7 +49,9 @@ public class MergedAlignmentReader implements AlignmentReader { List readers; List sequenceNames; - Map chrNameIndex; + + Map> readerChrNameMaps; + SAMFileHeader header; public MergedAlignmentReader(List readers) throws IOException { @@ -79,53 +81,64 @@ public Set getPlatforms() { Set platforms = new HashSet(); for (AlignmentReader reader : readers) { Set plf = reader.getPlatforms(); - if(plf != null){ + if (plf != null) { platforms.addAll(plf); } } return platforms; } - public SAMFileHeader getFileHeader(){ - if(this.header == null){ + public SAMFileHeader getFileHeader() { + if (this.header == null) { this.header = loadHeaders(); } return this.header; } /** - * Return the merged list of all sequence names, maintaining order. + * Return the merged list of all sequence names, maintaining order. As sequence name conventions might + * vary between bam files ("1" vs "chr1", etc), the loaded genome canonical name is used for the sequence + * names of the merged bams. Consequentally we need to keep a seq name map per bam file (reader) to substitute + * back when querying.* * * * * @return */ public void loadSequenceNames() throws IOException { - // Use a set for quick comparison + + Genome genome = GenomeManager.getInstance().getCurrentGenome(); + + readerChrNameMaps = new HashMap<>(); + LinkedHashSet names = new LinkedHashSet(50); for (AlignmentReader reader : readers) { - names.addAll(reader.getSequenceNames()); - } - sequenceNames = new ArrayList(names); - Genome genome = GenomeManager.getInstance().getCurrentGenome(); - chrNameIndex = new HashMap(sequenceNames.size()); - for (int i = 0; i < sequenceNames.size(); i++) { - final String seqName = sequenceNames.get(i); - String chr = genome == null ? seqName : genome.getCanonicalChrName(seqName); - chrNameIndex.put(chr, i); + Map chrNameMap = new HashMap<>(); + + List readerSequenceNames = reader.getSequenceNames(); + for (String seq : readerSequenceNames) { + String chr = genome.getCanonicalChrName(seq); + names.add(chr); + + chrNameMap.put(chr, seq); + } + readerChrNameMaps.put(reader, chrNameMap); } + sequenceNames = new ArrayList<>(names); + + } - private SAMFileHeader loadHeaders(){ + private SAMFileHeader loadHeaders() { List headersList = new ArrayList(); SAMFileHeader.SortOrder sortOrder = null; - for(AlignmentReader reader: readers){ + for (AlignmentReader reader : readers) { SAMFileHeader curHeader = reader.getFileHeader(); - if(curHeader != null) { + if (curHeader != null) { headersList.add(curHeader); sortOrder = curHeader.getSortOrder(); } } - if(sortOrder != null){ + if (sortOrder != null) { SamFileHeaderMerger headerMerger = new SamFileHeaderMerger(sortOrder, headersList, true); return headerMerger.getMergedHeader(); } @@ -163,7 +176,8 @@ private void create(String chr, int start, int end, boolean contained) throws IO if (iterate) { iter = reader.iterator(); } else { - iter = reader.query(chr, start, end, contained); + String seq = readerChrNameMaps.get(reader).get(chr); + iter = reader.query(seq, start, end, contained); } allIterators.add(iter); if (iter.hasNext()) { @@ -231,18 +245,8 @@ class AlignmentStartComparator implements Comparator { public int compare(RecordIterWrapper wrapper1, RecordIterWrapper wrapper2) { Alignment a1 = wrapper1.nextRecord; Alignment a2 = wrapper2.nextRecord; + return a1.getAlignmentStart() - a2.getAlignmentStart(); - Integer idx1 = chrNameIndex.get(a1.getChr()); - Integer idx2 = chrNameIndex.get(a2.getChr()); - if(idx1==null) idx1 = Integer.MAX_VALUE; - if(idx2== null) idx2 = Integer.MAX_VALUE; // Put these records at the end. - if (idx1 > idx2) { - return 1; - } else if (idx1 < idx2) { - return -1; - } else { - return a1.getAlignmentStart() - a2.getAlignmentStart(); - } } } } From bc32f74d6f6817fdb1ca967397f5668524282c0a Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Fri, 16 Dec 2022 18:49:02 -0800 Subject: [PATCH 018/116] npe check --- .../java/org/broad/igv/sam/reader/MergedAlignmentReader.java | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/src/main/java/org/broad/igv/sam/reader/MergedAlignmentReader.java b/src/main/java/org/broad/igv/sam/reader/MergedAlignmentReader.java index f1794efcd7..e7a8b3dc90 100644 --- a/src/main/java/org/broad/igv/sam/reader/MergedAlignmentReader.java +++ b/src/main/java/org/broad/igv/sam/reader/MergedAlignmentReader.java @@ -176,7 +176,7 @@ private void create(String chr, int start, int end, boolean contained) throws IO if (iterate) { iter = reader.iterator(); } else { - String seq = readerChrNameMaps.get(reader).get(chr); + String seq = readerChrNameMaps.containsKey(reader) ? readerChrNameMaps.get(reader).get(chr) : chr; iter = reader.query(seq, start, end, contained); } allIterators.add(iter); From b6337613dff7a6e46d35316c3d9d3876240665d8 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Fri, 16 Dec 2022 21:28:16 -0800 Subject: [PATCH 019/116] Fix alignment sort order for merged bams --- .../igv/sam/reader/MergedAlignmentReader.java | 8 +++-- .../sam/reader/MergedAlignmentReaderTest.java | 30 +++++++++++++++++-- 2 files changed, 34 insertions(+), 4 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/reader/MergedAlignmentReader.java b/src/main/java/org/broad/igv/sam/reader/MergedAlignmentReader.java index e7a8b3dc90..f61a393bd6 100644 --- a/src/main/java/org/broad/igv/sam/reader/MergedAlignmentReader.java +++ b/src/main/java/org/broad/igv/sam/reader/MergedAlignmentReader.java @@ -245,8 +245,12 @@ class AlignmentStartComparator implements Comparator { public int compare(RecordIterWrapper wrapper1, RecordIterWrapper wrapper2) { Alignment a1 = wrapper1.nextRecord; Alignment a2 = wrapper2.nextRecord; - return a1.getAlignmentStart() - a2.getAlignmentStart(); - + int chrCompare = a1.getChr().compareTo(a2.getChr()); + if (chrCompare != 0) { + return chrCompare; + } else { + return a1.getAlignmentStart() - a2.getAlignmentStart(); + } } } } diff --git a/src/test/java/org/broad/igv/sam/reader/MergedAlignmentReaderTest.java b/src/test/java/org/broad/igv/sam/reader/MergedAlignmentReaderTest.java index 471924d860..3a4cbf8808 100644 --- a/src/test/java/org/broad/igv/sam/reader/MergedAlignmentReaderTest.java +++ b/src/test/java/org/broad/igv/sam/reader/MergedAlignmentReaderTest.java @@ -42,6 +42,7 @@ import java.util.Map; import static junit.framework.Assert.assertEquals; +import static junit.framework.Assert.assertTrue; /** * @author jacob @@ -49,7 +50,7 @@ */ public class MergedAlignmentReaderTest extends AbstractHeadlessTest { - public static String[] generateRepLargebamsList(File listFile) throws IOException{ + public static String[] generateRepLargebamsList(File listFile) throws IOException { listFile.delete(); listFile.deleteOnExit(); String listPath = listFile.getPath(); @@ -57,7 +58,8 @@ public static String[] generateRepLargebamsList(File listFile) throws IOExceptio return IGVToolsTest.generateRepLargebamsList(listPath, "HG00171.hg18.bam", 2); } - @Test @Ignore("Requires largedata bundle") + @Test + @Ignore("Requires largedata bundle") public void testSimpleRead() throws Exception { File listFile = new File(TestUtils.LARGE_DATA_DIR, "2largebams.bam.list"); @@ -109,4 +111,28 @@ public void testSimpleRead() throws Exception { } } + + @Test + public void testSortOrder() throws Exception { + String listPath = TestUtils.DATA_DIR + "bam/test.unindexed.bam.list"; + + AlignmentReader mergedReader = AlignmentReaderFactory.getBamListReader(listPath, false); + CloseableIterator iter = mergedReader.iterator(); + + int lastPosition = 0; + String lastChr = ""; + while (iter.hasNext()) { + Alignment a = iter.next(); + String chr = a.getChr(); + int pos = a.getAlignmentStart(); + assertTrue(chr.compareTo(lastChr) >= 0); + if(lastChr.equals(chr)) { + assertTrue(pos >= lastPosition); + } + lastChr = chr; + lastPosition = pos; + } + iter.close(); + } + } From 639f20017cec098405e014f2f1c12a4a12530e1d Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Fri, 16 Dec 2022 21:41:09 -0800 Subject: [PATCH 020/116] Account for chr name aliasing when sorting merged bam alignments --- .../org/broad/igv/sam/reader/MergedAlignmentReader.java | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/reader/MergedAlignmentReader.java b/src/main/java/org/broad/igv/sam/reader/MergedAlignmentReader.java index f61a393bd6..e2d2f2a27f 100644 --- a/src/main/java/org/broad/igv/sam/reader/MergedAlignmentReader.java +++ b/src/main/java/org/broad/igv/sam/reader/MergedAlignmentReader.java @@ -46,6 +46,7 @@ public class MergedAlignmentReader implements AlignmentReader { private static Logger log = LogManager.getLogger(MergedAlignmentReader.class); + private final Genome genome; List readers; List sequenceNames; @@ -56,6 +57,7 @@ public class MergedAlignmentReader implements AlignmentReader { public MergedAlignmentReader(List readers) throws IOException { this.readers = readers; + this.genome = GenomeManager.getInstance().getCurrentGenome(); loadSequenceNames(); } @@ -105,8 +107,6 @@ public SAMFileHeader getFileHeader() { */ public void loadSequenceNames() throws IOException { - Genome genome = GenomeManager.getInstance().getCurrentGenome(); - readerChrNameMaps = new HashMap<>(); LinkedHashSet names = new LinkedHashSet(50); @@ -245,7 +245,7 @@ class AlignmentStartComparator implements Comparator { public int compare(RecordIterWrapper wrapper1, RecordIterWrapper wrapper2) { Alignment a1 = wrapper1.nextRecord; Alignment a2 = wrapper2.nextRecord; - int chrCompare = a1.getChr().compareTo(a2.getChr()); + int chrCompare = genome.getCanonicalChrName(a1.getChr()).compareTo(genome.getCanonicalChrName(a2.getChr())); if (chrCompare != 0) { return chrCompare; } else { From 8bbd1cdb60976d0eb1964ce1f718994a04646110 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 21 Dec 2022 21:51:30 -0800 Subject: [PATCH 021/116] Add preference to perform "group" menu action on all alignmnet tracks at once. See issue #1275 * WORK IN PROGRESS * --- .../java/org/broad/igv/prefs/Constants.java | 1 + .../org/broad/igv/sam/AlignmentTrack.java | 9 +++++ .../org/broad/igv/sam/AlignmentTrackMenu.java | 39 +++++++++++++++---- .../java/org/broad/igv/util/HttpUtils.java | 18 +++++++++ .../org/broad/igv/prefs/preferences.tab | 1 + 5 files changed, 60 insertions(+), 8 deletions(-) diff --git a/src/main/java/org/broad/igv/prefs/Constants.java b/src/main/java/org/broad/igv/prefs/Constants.java index ce0f93e133..8723324578 100644 --- a/src/main/java/org/broad/igv/prefs/Constants.java +++ b/src/main/java/org/broad/igv/prefs/Constants.java @@ -145,6 +145,7 @@ private Constants() { public static final String SAM_COLOR_BY_TAG = "SAM.COLOR_BY_TAG"; public static final String SAM_SORT_BY_TAG = "SAM.SORT_BY_TAG"; public static final String SAM_GROUP_BY_TAG = "SAM.GROUP_BY_TAG"; + public static final String SAM_GROUP_ALL = "SAM.GROUP_ALL"; public static final String SAM_LINK_BY_TAGS = "SAM.LINK_BY_TAGS"; public static final String SAM_GROUP_BY_POS = "SAM.GROUP_BY_POS"; public static final String SAM_BISULFITE_CONTEXT = "SAM.BISULFITE_CONTEXT"; diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrack.java b/src/main/java/org/broad/igv/sam/AlignmentTrack.java index 117d9c0511..9d0596fbf3 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrack.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrack.java @@ -1340,6 +1340,7 @@ public static class RenderOptions implements Cloneable, Persistable { private Range groupByPos; private Boolean invertSorting; private boolean invertGroupSorting; + private boolean groupAll; private Boolean showInsertionMarkers; private Boolean hideSmallIndels; private Integer smallIndelThreshold; @@ -1426,6 +1427,10 @@ void setInvertGroupSorting(boolean invertGroupSorting) { this.invertGroupSorting = invertGroupSorting; } + public void setGroupAll(boolean groupAll) { + this.groupAll = groupAll; + } + void setLinkByTag(String linkByTag) { this.linkByTag = linkByTag; } @@ -1582,6 +1587,10 @@ public boolean isInvertGroupSorting() { return invertGroupSorting; } + public boolean isGroupAll() { + return groupAll; + } + public String getLinkByTag() { return linkByTag; } diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index 13969b1dcf..88caf3c2f2 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -9,6 +9,7 @@ import org.broad.igv.lists.GeneList; import org.broad.igv.logging.LogManager; import org.broad.igv.logging.Logger; +import org.broad.igv.prefs.Constants; import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.sashimi.SashimiPlot; import org.broad.igv.session.Session; @@ -211,8 +212,7 @@ private void addHaplotype(TrackClickEvent e) { boolean success = haplotypeUtils.clusterAlignments(frame.getChrName(), start, end, nClusters); if (success) { - alignmentTrack.groupAlignments(AlignmentTrack.GroupOption.HAPLOTYPE, null, null); - alignmentTrack.repaint(); + groupAlignments(AlignmentTrack.GroupOption.HAPLOTYPE, null, null); } //dataManager.sortRows(SortOption.HAPLOTYPE, frame, (end + start) / 2, null); @@ -338,6 +338,9 @@ private JCheckBoxMenuItem getExperimentTypeMenuItem(String label, final Alignmen } void addGroupMenuItem(final TrackClickEvent te) {//ReferenceFrame frame) { + + + final MouseEvent me = te.getMouseEvent(); ReferenceFrame frame = te.getFrame(); if (frame == null) { @@ -363,7 +366,8 @@ void addGroupMenuItem(final TrackClickEvent te) {//ReferenceFrame frame) { JCheckBoxMenuItem mi = new JCheckBoxMenuItem(option.label); mi.setSelected(renderOptions.getGroupByOption() == option); mi.addActionListener(aEvt -> { - alignmentTrack.groupAlignments(option, null, null); + groupAlignments(option, null, null); + }); groupMenu.add(mi); group.add(mi); @@ -374,9 +378,9 @@ void addGroupMenuItem(final TrackClickEvent te) {//ReferenceFrame frame) { String tag = MessageUtils.showInputDialog("Enter tag", renderOptions.getGroupByTag()); if (tag != null) { if (tag.trim().length() > 0) { - alignmentTrack.groupAlignments(AlignmentTrack.GroupOption.TAG, tag, null); + groupAlignments(AlignmentTrack.GroupOption.TAG, tag, null); } else { - alignmentTrack.groupAlignments(AlignmentTrack.GroupOption.NONE, null, null); + groupAlignments(AlignmentTrack.GroupOption.NONE, null, null); } } @@ -399,7 +403,7 @@ void addGroupMenuItem(final TrackClickEvent te) {//ReferenceFrame frame) { ":" + Globals.DECIMAL_FORMAT.format(1 + chromStart)); newGroupByPosOption.addActionListener(aEvt -> { Range groupByPos = new Range(chrom, chromStart, chromStart + 1); - alignmentTrack.groupAlignments(AlignmentTrack.GroupOption.BASE_AT_POS, null, groupByPos); + groupAlignments(AlignmentTrack.GroupOption.BASE_AT_POS, null, groupByPos); }); groupMenu.add(newGroupByPosOption); group.add(newGroupByPosOption); @@ -415,6 +419,15 @@ void addGroupMenuItem(final TrackClickEvent te) {//ReferenceFrame frame) { }); groupMenu.add(invertGroupNameSortingOption); + JCheckBoxMenuItem groupAllOption = new JCheckBoxMenuItem("Reverse group order"); + groupAllOption.setSelected(renderOptions.isInvertGroupSorting()); + groupAllOption.addActionListener(aEvt -> { + renderOptions.setGroupAll(!renderOptions.isGroupAll()); + dataManager.packAlignments(renderOptions); + alignmentTrack.repaint(); + }); + groupMenu.add(invertGroupNameSortingOption); + add(groupMenu); } @@ -586,7 +599,7 @@ void addColorByMenuItem() { mappings.put("SMRT CCS fwd-strand aligned IPD", AlignmentTrack.ColorOption.SMRT_CCS_FWD_IPD); mappings.put("SMRT CCS fwd-strand aligned PW", AlignmentTrack.ColorOption.SMRT_CCS_FWD_PW); mappings.put("SMRT CCS rev-strand aligned IPD", AlignmentTrack.ColorOption.SMRT_CCS_REV_IPD); - mappings.put("SMRT CCS rev-strand aligned PW",AlignmentTrack.ColorOption.SMRT_CCS_REV_PW); + mappings.put("SMRT CCS rev-strand aligned PW", AlignmentTrack.ColorOption.SMRT_CCS_REV_PW); colorMenu.addSeparator(); for (Map.Entry el : mappings.entrySet()) { JRadioButtonMenuItem mi = getColorMenuItem(el.getKey(), el.getValue()); @@ -1184,7 +1197,7 @@ private void splitScreenMate(ReferenceFrame frame, Alignment alignment) { GeneList geneList = new GeneList(listName.toString(), loci, false); currentSession.setCurrentGeneList(geneList); - + Comparator geneListComparator = (n0, n1) -> { ReferenceFrame f0 = FrameManager.getFrame(n0); ReferenceFrame f1 = FrameManager.getFrame(n1); @@ -1251,6 +1264,16 @@ private void setLinkByTag(boolean linkReads, String tag) { repaint(); } + private void groupAlignments(AlignmentTrack.GroupOption option, String tag, Range pos) { + if (alignmentTrack.getPreferences().getAsBoolean(SAM_GROUP_ALL)) { + for(AlignmentTrack t : IGV.getInstance().getAlignmentTracks()) { + t.groupAlignments(option, tag, pos); + } + } else { + alignmentTrack.groupAlignments(option, tag, pos); + } + } + /** * Listener for deselecting one component when another is selected diff --git a/src/main/java/org/broad/igv/util/HttpUtils.java b/src/main/java/org/broad/igv/util/HttpUtils.java index 47fbd7ece0..bfa592479f 100644 --- a/src/main/java/org/broad/igv/util/HttpUtils.java +++ b/src/main/java/org/broad/igv/util/HttpUtils.java @@ -197,6 +197,11 @@ public static URL createURL(String urlString) throws MalformedURLException { */ public static String mapURL(String urlString) throws MalformedURLException { + // Check explicit mappings first + if(urlMappings.containsKey(urlString)) { + return urlMappings.get(urlString); + } + if (urlString.startsWith("htsget://")) { urlString = urlString.replace("htsget://", "https://"); } else if (urlString.startsWith("gs://")) { @@ -1189,4 +1194,17 @@ public long getMaxAge() { return maxAge; } } + + private static Map urlMappings; + static { + // mutable map + urlMappings = new HashMap<>(); + urlMappings.put("https://s3.amazonaws.com/igv.broadinstitute.org/genomes/seq/hg19/hg19.fasta", "https://igv.genepattern.org/genomes/seq/hg19/hg19.fasta"); + urlMappings.put("https://s3.amazonaws.com/igv.broadinstitute.org/genomes/seq/hg19/hg19.fasta.fai", "https://igv.genepattern.org/genomes/seq/hg19/hg19.fasta.fai"); + urlMappings.put("https://s3.amazonaws.com/igv.broadinstitute.org/genomes/seq/hg19/cytoBand.txt", "https://igv.genepattern.org/genomes/seq/hg19/cytoBand.txt"); + urlMappings.put("https://s3.amazonaws.com/igv.broadinstitute.org/genomes/seq/hg38/hg38.fa", "https://igv.genepattern.org/genomes/seq/hg38/hg38.fa"); + urlMappings.put("https://s3.amazonaws.com/igv.broadinstitute.org/genomes/seq/hg38/hg38.fa.fai", "https://igv.genepattern.org/genomes/seq/hg38/hg38.fa.fai"); + } + + } diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index 9129618993..995013b641 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -88,6 +88,7 @@ SAM.COLOR_BY Color alignments by select NONE|READ_STRAND|FIRST_OF_PAIR_STRAND|PA SAM.COLOR_BY_TAG Color by TAG string SAM.GROUP_OPTION Group alignments by select NONE|STRAND|SAMPLE|READ_GROUP|LIBRARY|FIRST_OF_PAIR_STRAND|TAG|PAIR_ORIENTATION|MATE_CHROMOSOME|SV_ALIGNMENT|SUPPLEMENTARY|BASE_AT_POS|MOVIE|ZMW|HAPLOTYPE|READ_ORDER|LINKED|PHASE|MAPPING_QUALITY SAM.GROUP_BY_TAG Group by TAG string +SAM.GROUP_ALL Sync alignment track grouping boolean FALSE SAM.MAX_VISIBLE_RANGE Visibility range threshold (kb) float 30 Range at which alignments become visibile SAM.QUALITY_THRESHOLD Mapping quality threshold int 0 Hide alignments with MQ lower than this value SAM.ALIGNMENT_SCORE_THRESHOLD Alignment score threshold int 0 From 26ac437182e83abdc3c02d191fcb5c694a2b5daa Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 22 Dec 2022 10:31:35 -0800 Subject: [PATCH 022/116] set default for SMRT kinetics preference --- src/main/resources/org/broad/igv/prefs/preferences.tab | 2 +- test/data/bam/platinum.bam.list | 3 +++ 2 files changed, 4 insertions(+), 1 deletion(-) create mode 100644 test/data/bam/platinum.bam.list diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index 995013b641..5d6a2707a7 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -241,7 +241,7 @@ CIRC_VIEW_PORT CircView port integer 60152 SAM.SHOW_JUNCTION_FLANKINGREGIONS FALSE SAM.JUNCTION_MIN_FLANKING_WIDTH 0 SAM.JUNCTION_MIN_COVERAGE 1 - +SMRT_KINETICS.SHOW_OPTIONS FALSE SCORE_VARIANTS FALSE diff --git a/test/data/bam/platinum.bam.list b/test/data/bam/platinum.bam.list new file mode 100644 index 0000000000..3a6360b2df --- /dev/null +++ b/test/data/bam/platinum.bam.list @@ -0,0 +1,3 @@ +gs://genomics-public-data/platinum-genomes/bam/NA12889_S1.bam +gs://genomics-public-data/platinum-genomes/bam/NA12877_S1.bam +gs://genomics-public-data/platinum-genomes/bam/NA12878_S1.bam From 3a37c0c4d4fa0f88bea84321020060e74c83e3c8 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 22 Dec 2022 10:32:22 -0800 Subject: [PATCH 023/116] set default for SMRT kinetics preference --- src/main/resources/org/broad/igv/prefs/preferences.tab | 2 +- test/data/bam/platinum.bam.list | 3 +++ 2 files changed, 4 insertions(+), 1 deletion(-) create mode 100644 test/data/bam/platinum.bam.list diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index 9129618993..8c66e1e92c 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -240,7 +240,7 @@ CIRC_VIEW_PORT CircView port integer 60152 SAM.SHOW_JUNCTION_FLANKINGREGIONS FALSE SAM.JUNCTION_MIN_FLANKING_WIDTH 0 SAM.JUNCTION_MIN_COVERAGE 1 - +SMRT_KINETICS.SHOW_OPTIONS FALSE SCORE_VARIANTS FALSE diff --git a/test/data/bam/platinum.bam.list b/test/data/bam/platinum.bam.list new file mode 100644 index 0000000000..3a6360b2df --- /dev/null +++ b/test/data/bam/platinum.bam.list @@ -0,0 +1,3 @@ +gs://genomics-public-data/platinum-genomes/bam/NA12889_S1.bam +gs://genomics-public-data/platinum-genomes/bam/NA12877_S1.bam +gs://genomics-public-data/platinum-genomes/bam/NA12878_S1.bam From 548e34a455fa008bf19582c4de747b2410cfef5e Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 4 Jan 2023 21:03:46 -0800 Subject: [PATCH 024/116] Allow "alias.tab.txt" extension in addition to "alias.tab". Some plain text editors will append a ".txt" extension --- src/main/java/org/broad/igv/feature/genome/GenomeManager.java | 3 +++ 1 file changed, 3 insertions(+) diff --git a/src/main/java/org/broad/igv/feature/genome/GenomeManager.java b/src/main/java/org/broad/igv/feature/genome/GenomeManager.java index 444419056e..b43082b606 100644 --- a/src/main/java/org/broad/igv/feature/genome/GenomeManager.java +++ b/src/main/java/org/broad/igv/feature/genome/GenomeManager.java @@ -200,6 +200,9 @@ public Genome loadGenome(String genomePath, ProgressMonitor monitor) throws IOEx // Load user-defined chr aliases, if any. This is done last so they have priority try { String aliasPath = (new File(DirectoryManager.getGenomeCacheDirectory(), newGenome.getId() + "_alias.tab")).getAbsolutePath(); + if(!(new File(aliasPath)).exists()) { + aliasPath = (new File(DirectoryManager.getGenomeCacheDirectory(), newGenome.getId() + "_alias.tab.txt")).getAbsolutePath(); + } if ((new File(aliasPath)).exists()) { newGenome.addChrAliases(GenomeLoader.loadChrAliases(aliasPath)); } From 67f843a7eab6926bafa27779884d1e094f856aba Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 5 Jan 2023 12:01:42 -0800 Subject: [PATCH 025/116] Fix broken blat export --- .../java/org/broad/igv/feature/PSLRecord.java | 88 +++++++++++++++++-- .../broad/igv/feature/tribble/PSLCodec.java | 12 ++- test/data/psl/humanBlat.psl | 7 ++ 3 files changed, 94 insertions(+), 13 deletions(-) create mode 100644 test/data/psl/humanBlat.psl diff --git a/src/main/java/org/broad/igv/feature/PSLRecord.java b/src/main/java/org/broad/igv/feature/PSLRecord.java index b7c7cd0554..39400f6c9d 100644 --- a/src/main/java/org/broad/igv/feature/PSLRecord.java +++ b/src/main/java/org/broad/igv/feature/PSLRecord.java @@ -30,9 +30,35 @@ /** * @author jrobinso - * Date: 11/29/12 - * Time: 6:59 PM + * Date: 11/29/12 + * Time: 6:59 PM + *

+ *

+ * * 1. matches - Number of bases that match that aren't repeats + * * 2. misMatches - Number of bases that don't match + * * 3. repMatches - Number of bases that match but are part of repeats + * * 4. nCount - Number of 'N' bases + * * 5. qNumInsert - Number of inserts in query + * * 6. qBaseInsert - Number of bases inserted in query + * * 7. tNumInsert - Number of inserts in target + * * 8. tBaseInsert - Number of bases inserted in target + * * 9. strand - '+' or '-' for query strand. For translated alignments, second '+'or '-' is for genomic strand + * * 10. qName - Query sequence name + * * 11. qSize - Query sequence size + * * 12. qStart - Alignment start position in query + * * 13. qEnd - Alignment end position in query + * * 14. tName - Target sequence name + * * 15. tSize - Target sequence size + * * 16. tStart - Alignment start position in target + * * 17. tEnd - Alignment end position in target + * * 18. blockCount - Number of blocks in the alignment (a block contains no gaps) + * * 19. blockSizes - Comma-separated list of sizes of each block + * * 20. qStarts - Comma-separated list of starting positions of each block in query + * * 21. tStarts - Comma-separated list of starting positions of each block in target + * * + * * 59 9 0 0 1 823 1 96 +- FS_CONTIG_48080_1 1955 171 1062 chr22 47748585 13073589 13073753 2 48,20, 171,1042, 34674832,34674976,* * */ + public class PSLRecord extends BasicFeature { @@ -48,8 +74,11 @@ public class PSLRecord extends BasicFeature { private int ns; private int qGapBases; private int tGapBases; - private String text; + private String blockQueryStarts; + + private int qStart; + private int qEnd; public void setMatch(int match) { this.match = match; @@ -71,6 +100,14 @@ public void setTGapCount(int TNumInsert) { this.tGapCount = TNumInsert; } + public void setqStart(int qStart) { + this.qStart = qStart; + } + + public void setqEnd(int qEnd) { + this.qEnd = qEnd; + } + public void setQSize(int qSize) { this.qSize = qSize; } @@ -135,11 +172,50 @@ public int getTGapBases() { return tGapBases; } - public void setText(String text) { - this.text = text; + public void setBlockQueryStarts(String blockQueryStarts) { + this.blockQueryStarts = blockQueryStarts; } +// 128999999952YourSeq 1289128chr3 9364096243640974310119, 0 36409615, public String getText() { - return text; + StringBuffer buffer = new StringBuffer(); + buffer.append(match + "\t"); // 0 + buffer.append(misMatch + "\t"); // 1 + buffer.append(repMatch + "\t"); // 2 + buffer.append(ns + "\t"); // 3 + buffer.append(qGapCount + "\t"); // 4 + buffer.append(qGapBases + "\t"); // 5 + buffer.append(tGapCount + "\t"); // 6 + buffer.append(tGapBases + "\t"); // 7 + buffer.append((strand == Strand.POSITIVE ? '+' : '-') + "\t"); // 8 + buffer.append(name + "\t"); // 9 + buffer.append(qSize + "\t"); // 10 + buffer.append(qStart + "\t"); // 11 + buffer.append(qEnd + "\t"); // 12 + buffer.append(chr + "\t"); // 13 + buffer.append(tSize + "\t"); // 14 + buffer.append(start + "\t"); // 15 + buffer.append(end + "\t"); // 16 + buffer.append(exons.size() + "\t"); // 17 + + // block sizes // 18 + for (Exon exon : exons) { + buffer.append(exon.getLength() + ","); + } + buffer.append("\t"); + + buffer.append(blockQueryStarts + "\t"); // 19 + + // block target starts // 20 + for (Exon exon : exons) { + buffer.append(exon.getStart() + ","); + } + buffer.append("\t"); + + + /* + + */ + return buffer.toString(); } } diff --git a/src/main/java/org/broad/igv/feature/tribble/PSLCodec.java b/src/main/java/org/broad/igv/feature/tribble/PSLCodec.java index 00753f324a..38643ce58d 100644 --- a/src/main/java/org/broad/igv/feature/tribble/PSLCodec.java +++ b/src/main/java/org/broad/igv/feature/tribble/PSLCodec.java @@ -98,10 +98,6 @@ public PSLRecord decode(String line) { f = getPslRecord(tokens, genome); if (f == null) return null; - if(keepText) { - f.setText(line); - } - } catch (NumberFormatException e) { return null; } @@ -167,9 +163,6 @@ public static PSLRecord getPslRecord(String[] tokens, Genome genome) { for (int i = 0; i < startsBuffer.length; i++) { int exonSize = Integer.parseInt(exonSizes[i]); int exonStart = Integer.parseInt(startsBuffer[i]); - if (gNeg) { - exonStart = tSize - exonStart - exonSize; - } int exonEnd = exonStart + exonSize; Exon exon = new Exon(chr, exonStart, exonEnd, strand); f.addExon(exon); @@ -188,6 +181,8 @@ public static PSLRecord getPslRecord(String[] tokens, Genome genome) { int tGapCount = Integer.parseInt(tokens[6]); int tGapBases = Integer.parseInt(tokens[7]); int qSize = Integer.parseInt(tokens[10]); + int qStart = Integer.parseInt(tokens[11]); + int qEnd = Integer.parseInt(tokens[12]); float score = (1000.0f * (match + repMatch - misMatch - qGapCount - tGapCount)) / qSize; @@ -200,6 +195,9 @@ public static PSLRecord getPslRecord(String[] tokens, Genome genome) { f.setTGapCount(tGapCount); f.setTGapBases(tGapBases); f.setQSize(qSize); + f.setqStart(qStart); + f.setqEnd(qEnd); + f.setBlockQueryStarts(tokens[19]); f.setScore(score); diff --git a/test/data/psl/humanBlat.psl b/test/data/psl/humanBlat.psl new file mode 100644 index 0000000000..1858b111ef --- /dev/null +++ b/test/data/psl/humanBlat.psl @@ -0,0 +1,7 @@ +#hg38 query - ATTCTGCACAGTGACACTGACTTGAAGATATCTTGTATCCACTCCATCCATCCACGTGCATCTTGGGGAAAAATAGAGGAGGCAAGTCATGCATTAAATTATCAAGGTAATATATTTCA +119 0 0 0 0 0 0 0 + YourSeq 119 0 119 chr3 198295559 36409615 36409734 1 119, 0, 36409615, +27 0 0 0 0 0 1 145 + YourSeq 119 92 119 chr6 170805979 84433944 84434116 2 18,9, 92,110, 84433944,84434107, +23 0 0 0 0 0 0 0 - YourSeq 119 63 86 chr4 190214555 114187126 114187149 1 23, 33, 114187126, +24 0 0 0 1 1 1 2 + YourSeq 119 56 81 chr5 181538259 165141490 165141516 2 7,17, 56,64, 165141490,165141499, +23 0 0 0 1 1 0 0 + YourSeq 119 60 84 chr3 198295559 40347823 40347846 2 4,19, 60,65, 40347823,40347827, +21 0 0 0 0 0 0 0 - YourSeq 119 3 24 chr10 133797422 96204441 96204462 1 21, 95, 96204441, From 02287bf819652718ffac4f120f3b92dd54862472 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Mon, 9 Jan 2023 10:03:53 -0800 Subject: [PATCH 026/116] Updat info message for alignment track preferences --- src/main/resources/org/broad/igv/prefs/preferences.tab | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index 8c66e1e92c..288fc8ca02 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -70,7 +70,8 @@ CN_FREQ.AMP_THRESHOLD CN freq amplification threshold float 0.1 CN_FREQ.DEL_THRESHOLD CN freq deletion threshold float -0.1 #Alignments -info Settings for alignment tracks. See the RNA or Third Gen tabs to override for specific types. +info Default settings for alignment tracks. A subset of these properties are overriden for RNA and 3rd generation data. +info See the RNA or Third Gen tabs for those track types. ##Track Display SAM.SHOW_ALIGNMENT_TRACK Show alignment track boolean TRUE @@ -83,7 +84,6 @@ SAM.SAMPLING_WINDOW Sampling window size (bases) integer 50 SAM.MAX_LEVELS Number of reads per window integer 100 ##Alignment Track Options - SAM.COLOR_BY Color alignments by select NONE|READ_STRAND|FIRST_OF_PAIR_STRAND|PAIR_ORIENTATION|UNEXPECTED_PAIR|INSERT_SIZE|SAMPLE|READ_GROUP|LIBRARY|MOVIE|ZMW|BISULFITE|NOMESEQ|TAG|MAPPED_SIZE|LINK_STRAND|YC_TAG UNEXPECTED_PAIR SAM.COLOR_BY_TAG Color by TAG string SAM.GROUP_OPTION Group alignments by select NONE|STRAND|SAMPLE|READ_GROUP|LIBRARY|FIRST_OF_PAIR_STRAND|TAG|PAIR_ORIENTATION|MATE_CHROMOSOME|SV_ALIGNMENT|SUPPLEMENTARY|BASE_AT_POS|MOVIE|ZMW|HAPLOTYPE|READ_ORDER|LINKED|PHASE|MAPPING_QUALITY From 543f80d823de718b39f0856deab8c5af6179c068 Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Tue, 10 Jan 2023 12:45:11 -0800 Subject: [PATCH 027/116] Group all (#1277) * Updat info message for alignment track preferences * Add option to group all alignment tracks. Fixes #1275 --- .../java/org/broad/igv/prefs/Constants.java | 2 ++ .../org/broad/igv/sam/AlignmentTrackMenu.java | 30 +++++++++++++++---- .../org/broad/igv/prefs/preferences.tab | 7 +++-- 3 files changed, 31 insertions(+), 8 deletions(-) diff --git a/src/main/java/org/broad/igv/prefs/Constants.java b/src/main/java/org/broad/igv/prefs/Constants.java index ce0f93e133..787223c720 100644 --- a/src/main/java/org/broad/igv/prefs/Constants.java +++ b/src/main/java/org/broad/igv/prefs/Constants.java @@ -139,6 +139,8 @@ private Constants() { public static final String SAM_SORT_OPTION = "SAM.SORT_OPTION"; public static final String SAM_INVERT_SORT = "SAM.INVERT_SORT"; public static final String SAM_GROUP_OPTION = "SAM.GROUP_OPTION"; + + public static final String SAM_GROUP_ALL = "SAM.GROUP_ALL"; public static final String SAM_SHOW_ALL_BASES = "SAM.SHOW_ALL_BASES"; public static final String SAM_SHOW_MISMATCHES = "SAM.SHOW_MISMATCHES"; public static final String SAM_COLOR_BY = "SAM.COLOR_BY"; diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index 13969b1dcf..645ce451e7 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -9,6 +9,7 @@ import org.broad.igv.lists.GeneList; import org.broad.igv.logging.LogManager; import org.broad.igv.logging.Logger; +import org.broad.igv.prefs.Constants; import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.sashimi.SashimiPlot; import org.broad.igv.session.Session; @@ -211,7 +212,7 @@ private void addHaplotype(TrackClickEvent e) { boolean success = haplotypeUtils.clusterAlignments(frame.getChrName(), start, end, nClusters); if (success) { - alignmentTrack.groupAlignments(AlignmentTrack.GroupOption.HAPLOTYPE, null, null); + groupAlignments(AlignmentTrack.GroupOption.HAPLOTYPE, null, null); alignmentTrack.repaint(); } @@ -363,7 +364,7 @@ void addGroupMenuItem(final TrackClickEvent te) {//ReferenceFrame frame) { JCheckBoxMenuItem mi = new JCheckBoxMenuItem(option.label); mi.setSelected(renderOptions.getGroupByOption() == option); mi.addActionListener(aEvt -> { - alignmentTrack.groupAlignments(option, null, null); + groupAlignments(option, null, null); }); groupMenu.add(mi); group.add(mi); @@ -374,9 +375,9 @@ void addGroupMenuItem(final TrackClickEvent te) {//ReferenceFrame frame) { String tag = MessageUtils.showInputDialog("Enter tag", renderOptions.getGroupByTag()); if (tag != null) { if (tag.trim().length() > 0) { - alignmentTrack.groupAlignments(AlignmentTrack.GroupOption.TAG, tag, null); + groupAlignments(AlignmentTrack.GroupOption.TAG, tag, null); } else { - alignmentTrack.groupAlignments(AlignmentTrack.GroupOption.NONE, null, null); + groupAlignments(AlignmentTrack.GroupOption.NONE, null, null); } } @@ -399,7 +400,7 @@ void addGroupMenuItem(final TrackClickEvent te) {//ReferenceFrame frame) { ":" + Globals.DECIMAL_FORMAT.format(1 + chromStart)); newGroupByPosOption.addActionListener(aEvt -> { Range groupByPos = new Range(chrom, chromStart, chromStart + 1); - alignmentTrack.groupAlignments(AlignmentTrack.GroupOption.BASE_AT_POS, null, groupByPos); + groupAlignments(AlignmentTrack.GroupOption.BASE_AT_POS, null, groupByPos); }); groupMenu.add(newGroupByPosOption); group.add(newGroupByPosOption); @@ -415,9 +416,28 @@ void addGroupMenuItem(final TrackClickEvent te) {//ReferenceFrame frame) { }); groupMenu.add(invertGroupNameSortingOption); + JCheckBoxMenuItem groupAllOption = new JCheckBoxMenuItem("Group all tracks"); + groupAllOption.setSelected(alignmentTrack.getPreferences().getAsBoolean(SAM_GROUP_ALL)); + groupAllOption.addActionListener(aEvt -> { + alignmentTrack.getPreferences().put(SAM_GROUP_ALL, groupAllOption.getState()); + }); + groupMenu.add(groupAllOption); + add(groupMenu); } + private void groupAlignments(AlignmentTrack.GroupOption option, String tag, Range pos) { + + if(alignmentTrack.getPreferences().getAsBoolean(SAM_GROUP_ALL)) { + for(AlignmentTrack t : IGV.getInstance().getAlignmentTracks()) { + t.groupAlignments(option, tag, pos); + } + } else { + alignmentTrack.groupAlignments(option, tag, pos); + } + } + + /** * Sort menu */ diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index 8c66e1e92c..c76beca360 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -70,7 +70,8 @@ CN_FREQ.AMP_THRESHOLD CN freq amplification threshold float 0.1 CN_FREQ.DEL_THRESHOLD CN freq deletion threshold float -0.1 #Alignments -info Settings for alignment tracks. See the RNA or Third Gen tabs to override for specific types. +info Default settings for alignment tracks. A subset of these properties are overriden for RNA and 3rd generation data. +info See the RNA or Third Gen tabs for those track types. ##Track Display SAM.SHOW_ALIGNMENT_TRACK Show alignment track boolean TRUE @@ -83,7 +84,6 @@ SAM.SAMPLING_WINDOW Sampling window size (bases) integer 50 SAM.MAX_LEVELS Number of reads per window integer 100 ##Alignment Track Options - SAM.COLOR_BY Color alignments by select NONE|READ_STRAND|FIRST_OF_PAIR_STRAND|PAIR_ORIENTATION|UNEXPECTED_PAIR|INSERT_SIZE|SAMPLE|READ_GROUP|LIBRARY|MOVIE|ZMW|BISULFITE|NOMESEQ|TAG|MAPPED_SIZE|LINK_STRAND|YC_TAG UNEXPECTED_PAIR SAM.COLOR_BY_TAG Color by TAG string SAM.GROUP_OPTION Group alignments by select NONE|STRAND|SAMPLE|READ_GROUP|LIBRARY|FIRST_OF_PAIR_STRAND|TAG|PAIR_ORIENTATION|MATE_CHROMOSOME|SV_ALIGNMENT|SUPPLEMENTARY|BASE_AT_POS|MOVIE|ZMW|HAPLOTYPE|READ_ORDER|LINKED|PHASE|MAPPING_QUALITY @@ -252,7 +252,6 @@ SAM.BISULFITE_CONTEXT CG SAM.NOMESEQ_ENABLED FALSE SAM.COUNT_DELETED_BASES_COVERED FALSE SAM.SORT_OPTION NUCLEOTIDE -SAM.GROUP_OPTION NONE SAM.COLOR_BY UNEXPECTED_PAIR SAM.SHOW_GROUP_SEPARATOR TRUE SAM.REDUCED_MEMORY_MODE FALSE @@ -261,6 +260,8 @@ SAM.COLOR.C 0,0,255 SAM.COLOR.G 209,113,5 SAM.COLOR.N 182,182,182 SAM.COLOR.T 255,0,0 +SAM.GROUP_ALL FALSE +SAM.INVERT_SORT FALSE SEARCH_ZOOM TRUE From fa6aea65249a050aa837ff603ba980d072e391d8 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 12 Jan 2023 11:15:27 -0800 Subject: [PATCH 028/116] insure experimentType preferences, e.g. hiding junction track, get set even if # reads sampled is < threshold. See issue #1276 --- src/main/java/org/broad/igv/sam/AlignmentTrack.java | 8 +++----- src/main/java/org/broad/igv/sam/ReadStats.java | 2 +- 2 files changed, 4 insertions(+), 6 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrack.java b/src/main/java/org/broad/igv/sam/AlignmentTrack.java index 117d9c0511..0a0b4570b2 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrack.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrack.java @@ -348,11 +348,9 @@ public AlignmentTrack(ResourceLocator locator, AlignmentDataManager dataManager, } public void init() { - if (experimentType == null) { + if (experimentType == null || experimentType == ExperimentType.UNKOWN) { ExperimentType type = dataManager.inferType(); - if (type != null) { - setExperimentType(type); - } + setExperimentType(type); } } @@ -1020,7 +1018,7 @@ public void setViewAsPairs(boolean vAP) { } - public enum ExperimentType {OTHER, RNA, BISULFITE, THIRD_GEN} + public enum ExperimentType {OTHER, RNA, BISULFITE, THIRD_GEN, UNKOWN} class RenderRollback { diff --git a/src/main/java/org/broad/igv/sam/ReadStats.java b/src/main/java/org/broad/igv/sam/ReadStats.java index cf2981c509..14bd051722 100644 --- a/src/main/java/org/broad/igv/sam/ReadStats.java +++ b/src/main/java/org/broad/igv/sam/ReadStats.java @@ -134,7 +134,7 @@ private double[] downsample(DoubleArrayList list, int size) { public AlignmentTrack.ExperimentType inferType() { compute(); - if (readCount < 20) return null; // Not enough reads + if (readCount < 20) return AlignmentTrack.ExperimentType.UNKOWN; // Not enough reads if ((readLengthStdDev > 100 || medianReadLength > 1000) && averageCigarLength > 10) { // Cigar length to filter consensus reads return AlignmentTrack.ExperimentType.THIRD_GEN; } else if (medianRefToReadRatio > 5 || fracReadsWithNs > 0.01) { From 9b4af247f847fd5812433118a88555cce0191cbb Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 12 Jan 2023 19:05:28 -0800 Subject: [PATCH 029/116] bug fix --- src/main/java/org/broad/igv/prefs/Constants.java | 1 - 1 file changed, 1 deletion(-) diff --git a/src/main/java/org/broad/igv/prefs/Constants.java b/src/main/java/org/broad/igv/prefs/Constants.java index cc5336644d..787223c720 100644 --- a/src/main/java/org/broad/igv/prefs/Constants.java +++ b/src/main/java/org/broad/igv/prefs/Constants.java @@ -147,7 +147,6 @@ private Constants() { public static final String SAM_COLOR_BY_TAG = "SAM.COLOR_BY_TAG"; public static final String SAM_SORT_BY_TAG = "SAM.SORT_BY_TAG"; public static final String SAM_GROUP_BY_TAG = "SAM.GROUP_BY_TAG"; - public static final String SAM_GROUP_ALL = "SAM.GROUP_ALL"; public static final String SAM_LINK_BY_TAGS = "SAM.LINK_BY_TAGS"; public static final String SAM_GROUP_BY_POS = "SAM.GROUP_BY_POS"; public static final String SAM_BISULFITE_CONTEXT = "SAM.BISULFITE_CONTEXT"; From 990116866e9c46dd6947aa4b50585381bb20c891 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Fri, 20 Jan 2023 10:27:12 -0800 Subject: [PATCH 030/116] Make "Enter" from either url or index filed trigger "Load from url" ok button. See #1273 --- src/main/java/org/broad/igv/ui/util/LoadFromURLDialog.java | 5 +++++ 1 file changed, 5 insertions(+) diff --git a/src/main/java/org/broad/igv/ui/util/LoadFromURLDialog.java b/src/main/java/org/broad/igv/ui/util/LoadFromURLDialog.java index 5416daa0b7..b1588c29af 100644 --- a/src/main/java/org/broad/igv/ui/util/LoadFromURLDialog.java +++ b/src/main/java/org/broad/igv/ui/util/LoadFromURLDialog.java @@ -181,6 +181,11 @@ private void initComponents() { contentPane.add(dialogPane, BorderLayout.CENTER); pack(); setLocationRelativeTo(getOwner()); + + // Make "enter" trigger the ok button + fileField.addActionListener(e -> okButtonActionPerformed(e)); + indexField.addActionListener(e -> okButtonActionPerformed(e)); + this.rootPane.setDefaultButton(okButton); // JFormDesigner - End of component initialization //GEN-END:initComponents } From eb3edc7bbb03e1f409d9e9818af8a08c7c1fcc00 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Fri, 20 Jan 2023 16:15:43 -0800 Subject: [PATCH 031/116] Allow multiple URLs in load menu. Fixes #1273 --- .../igv/ui/action/LoadFromURLMenuAction.java | 125 +++++++----------- .../java/org/broad/igv/util/GoogleUtils.java | 3 +- 2 files changed, 48 insertions(+), 80 deletions(-) diff --git a/src/main/java/org/broad/igv/ui/action/LoadFromURLMenuAction.java b/src/main/java/org/broad/igv/ui/action/LoadFromURLMenuAction.java index e46c76bd3b..6dac928b86 100644 --- a/src/main/java/org/broad/igv/ui/action/LoadFromURLMenuAction.java +++ b/src/main/java/org/broad/igv/ui/action/LoadFromURLMenuAction.java @@ -29,12 +29,10 @@ */ package org.broad.igv.ui.action; +import org.broad.igv.Globals; import org.broad.igv.logging.*; -import org.broad.igv.exceptions.HttpResponseException; import org.broad.igv.feature.genome.GenomeManager; import org.broad.igv.util.GoogleUtils; -import org.broad.igv.oauth.OAuthProvider; -import org.broad.igv.oauth.OAuthUtils; import org.broad.igv.prefs.Constants; import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.session.SessionReader; @@ -43,18 +41,13 @@ import org.broad.igv.ui.util.LoadFromURLDialog; import org.broad.igv.ui.util.MessageUtils; import org.broad.igv.util.AmazonUtils; -import org.broad.igv.util.HttpUtils; import org.broad.igv.util.LongRunningTask; import org.broad.igv.util.ResourceLocator; import javax.swing.*; import java.awt.*; import java.awt.event.ActionEvent; -import java.io.IOException; -import java.io.InputStream; -import java.util.Arrays; -import java.util.HashMap; -import java.util.Map; +import java.util.ArrayList; import static org.broad.igv.util.AmazonUtils.isObjectAccessible; @@ -64,9 +57,7 @@ public class LoadFromURLMenuAction extends MenuAction { static Logger log = LogManager.getLogger(LoadFilesMenuAction.class); - public static final String LOAD_FROM_DAS = "Load from DAS..."; public static final String LOAD_FROM_URL = "Load from URL..."; - public static final String LOAD_FILE_AND_INDEX_FROM_URLS = "Load file and index from URLs..."; public static final String LOAD_GENOME_FROM_URL = "Load Genome from URL..."; private IGV igv; @@ -87,59 +78,58 @@ public void actionPerformed(ActionEvent e) { if (!dlg.isCanceled()) { - String inputURL = dlg.getFileURL(); + String inputURLs = dlg.getFileURL(); + if (inputURLs != null && inputURLs.trim().length() > 0) { - if (inputURL != null && inputURL.trim().length() > 0) { - - final String url = mapURL(inputURL.trim()); - - if (url.startsWith("s3://")) { - checkAWSAccessbility(url); - } - - if (SessionReader.isSessionFile(url)) { + String[] inputs = Globals.whitespacePattern.split(inputURLs.trim()); + checkURLs(inputs); + if (inputs.length == 1 && SessionReader.isSessionFile(inputs[0])) { + // Session URL + String url = inputs[0]; + if (url.startsWith("s3://")) { + checkAWSAccessbility(url); + } try { LongRunningTask.submit(() -> this.igv.loadSession(url, null)); } catch (Exception ex) { MessageUtils.showMessage("Error loading url: " + url + " (" + ex.toString() + ")"); } } else { - ResourceLocator rl = new ResourceLocator(url.trim()); - - if (dlg.getIndexURL() != null) { - String indexUrl = dlg.getIndexURL().trim(); - - if (GoogleUtils.isGoogleURL(indexUrl)) { - enableGoogleMenu(); + // Files, possibly indexed + String[] indexes = null; + String indexURLs = dlg.getIndexURL(); + if (indexURLs != null && indexURLs.trim().length() > 0) { + indexes = Globals.whitespacePattern.split(indexURLs.trim()); + if (indexes.length != inputs.length) { + throw new RuntimeException("The number of Index URLs must equal the number of File URLs"); } - - rl.setIndexPath(indexUrl); + checkURLs(indexes); } - igv.loadTracks(Arrays.asList(rl)); + ArrayList locators = new ArrayList<>(); + for (int i = 0; i < inputs.length; i++) { + String url = inputs[i]; + ResourceLocator rl = new ResourceLocator(url.trim()); + if (indexes != null) { + String indexUrl = indexes[i]; + rl.setIndexPath(indexUrl); + } + locators.add(rl); + } + igv.loadTracks(locators); } } } - } else if ((e.getActionCommand().equalsIgnoreCase(LOAD_FROM_DAS))) { - String url = JOptionPane.showInputDialog(IGV.getInstance().getMainFrame(), ta, "Enter DAS feature source URL", - JOptionPane.QUESTION_MESSAGE); - if (url != null && url.trim().length() > 0) { - ResourceLocator rl = new ResourceLocator(url.trim()); - rl.setFormat("das"); - igv.loadTracks(Arrays.asList(rl)); - } } else if ((e.getActionCommand().equalsIgnoreCase(LOAD_GENOME_FROM_URL))) { + String url = JOptionPane.showInputDialog(IGV.getInstance().getMainFrame(), ta, "Enter URL to .genome or FASTA file", JOptionPane.QUESTION_MESSAGE); + if (url != null && url.trim().length() > 0) { - if (url.startsWith("s3://")) { - checkAWSAccessbility(url); - } else if (url.startsWith("ftp://")) { - MessageUtils.showMessage("FTP protocol is not supported"); - } + url = url.trim(); try { - url = mapURL(url); - GenomeManager.getInstance().loadGenome(url.trim(), null); + checkURLs(new String[]{url}); + GenomeManager.getInstance().loadGenome(url, null); } catch (Exception e1) { MessageUtils.showMessage("Error loading genome: " + e1.getMessage()); } @@ -148,16 +138,19 @@ public void actionPerformed(ActionEvent e) { } } - private String mapURL(String url) { - - url = url.trim(); - if (GoogleUtils.isGoogleURL(url)) { - enableGoogleMenu(); + private void checkURLs(String[] urls) { + for (String url : urls) { + if (url.startsWith("s3://")) { + checkAWSAccessbility(url); + } else if (GoogleUtils.isGoogleURL(url)) { + enableGoogleMenu(); + } else if (url.startsWith("ftp://")) { + MessageUtils.showMessage("FTP protocol is not supported"); + } } - - return url; } + private void enableGoogleMenu() { if (!PreferencesManager.getPreferences().getAsBoolean(Constants.ENABLE_GOOGLE_MENU)) { @@ -184,31 +177,5 @@ private void checkAWSAccessbility(String url) { AmazonUtils.checkLogin(); } } - - private boolean ping(String url) { - InputStream is = null; - try { - Map params = new HashMap(); - params.put("Range", "bytes=0-10"); - byte[] buffer = new byte[10]; - is = HttpUtils.getInstance().openConnectionStream(HttpUtils.createURL(url), params); - is.read(buffer); - is.close(); - } catch (HttpResponseException e1) { - MessageUtils.showMessage(e1.getMessage()); - return false; - } catch (IOException e) { - log.error(e); - - } finally { - if (is != null) try { - is.close(); - } catch (IOException e) { - e.printStackTrace(); - } - } - - return true; - } } diff --git a/src/main/java/org/broad/igv/util/GoogleUtils.java b/src/main/java/org/broad/igv/util/GoogleUtils.java index 079f724c3c..c8f47de7bf 100644 --- a/src/main/java/org/broad/igv/util/GoogleUtils.java +++ b/src/main/java/org/broad/igv/util/GoogleUtils.java @@ -39,7 +39,8 @@ public static boolean isGoogleDrive(String url) { public static boolean isGoogleStorageURL(String url) { return url != null && - (url.startsWith("https://www.googleapis.com/storage") || + (url.startsWith("gs://") || + url.startsWith("https://www.googleapis.com/storage") || url.startsWith("https://storage.cloud.google.com") || url.startsWith("https://storage.googleapis.com")); } From a41b0296aa4526232dc64bb41332cfddf15b7274 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Fri, 20 Jan 2023 16:26:39 -0800 Subject: [PATCH 032/116] Skip setting user preferences if in batch mode (command runner or port command). See issue #1271 --- .../org/broad/igv/prefs/IGVPreferences.java | 32 ++++++++++++------- 1 file changed, 20 insertions(+), 12 deletions(-) diff --git a/src/main/java/org/broad/igv/prefs/IGVPreferences.java b/src/main/java/org/broad/igv/prefs/IGVPreferences.java index da1ba04361..325bff1ab2 100644 --- a/src/main/java/org/broad/igv/prefs/IGVPreferences.java +++ b/src/main/java/org/broad/igv/prefs/IGVPreferences.java @@ -262,19 +262,27 @@ private void clearCaches() { mutationColorScheme = null; } + /** + * Update preference. This command is ignored if in batch mode * + * @param key + * @param value + */ public void put(String key, String value) { - key = key.trim(); - // Explicitly setting removes override - overrideKeys.remove(key); + if (!Globals.isBatch()) { + key = key.trim(); - if (value == null || value.trim().length() == 0) { - userPreferences.remove(key); - } else { - userPreferences.put(key, value); + // Explicitly setting removes override + overrideKeys.remove(key); + + if (value == null || value.trim().length() == 0) { + userPreferences.remove(key); + } else { + userPreferences.put(key, value); + } + updateCaches(key, value); + IGVEventBus.getInstance().post(new PreferencesChangeEvent()); } - updateCaches(key, value); - IGVEventBus.getInstance().post(new PreferencesChangeEvent()); } public void put(String key, boolean b) { @@ -358,9 +366,9 @@ private void checkForCommandListenerChanges(Map updatedPreferenc private void checkForAttributePanelChanges(Map updatedPreferenceMap) { if (updatedPreferenceMap.containsKey(SHOW_ATTRIBUTE_VIEWS_KEY) || updatedPreferenceMap.containsKey(SHOW_DEFAULT_TRACK_ATTRIBUTES)) { - if(IGV.hasInstance()) { - IGV.getInstance().revalidateTrackPanels(); - } + if (IGV.hasInstance()) { + IGV.getInstance().revalidateTrackPanels(); + } } } From 0e41fbf1bcfd7c41005de665d16091b0fbb24481 Mon Sep 17 00:00:00 2001 From: Louis Bergelson Date: Sat, 21 Jan 2023 00:36:58 -0500 Subject: [PATCH 033/116] Add feature threshold indicator to zoom widget (#1196) * implementing getVisibilityWindow() * Add feature threshold indicator to zoom widget * Add an indicator to show which zoom level will start dispalying features/reads * closes https://github.com/igvteam/igv/issues/1192 * Reduce transparency value for zoom slider viz window indicator to "25". Update text session xml * fix exception when feature window size was larger than the contig length * extracted a log2 function instead of using a wierd static log2 constant everywhere Co-authored-by: jrobinso <933148+jrobinso@users.noreply.github.com> --- src/main/java/org/broad/igv/Globals.java | 6 ++-- .../java/org/broad/igv/data/seg/FreqData.java | 2 +- .../org/broad/igv/sam/AlignmentTrack.java | 7 +++- .../java/org/broad/igv/sam/CoverageTrack.java | 7 +++- .../igv/sashimi/SashimiJunctionRenderer.java | 2 +- .../tools/converters/ExpressionFormatter.java | 2 +- .../org/broad/igv/track/AbstractTrack.java | 2 +- .../broad/igv/ui/panel/ReferenceFrame.java | 7 ++-- .../broad/igv/ui/panel/ZoomSliderPanel.java | 33 ++++++++++++++++++- .../base_mods/HIFI_basemodifictions.xml | 14 ++++---- 10 files changed, 62 insertions(+), 20 deletions(-) diff --git a/src/main/java/org/broad/igv/Globals.java b/src/main/java/org/broad/igv/Globals.java index c2737439ea..502eebaf88 100644 --- a/src/main/java/org/broad/igv/Globals.java +++ b/src/main/java/org/broad/igv/Globals.java @@ -26,9 +26,7 @@ package org.broad.igv; import org.broad.igv.logging.*; -import org.broad.igv.renderer.SequenceRenderer; -import java.io.IOException; import java.text.DecimalFormat; import java.util.*; import java.util.List; @@ -88,7 +86,6 @@ public class Globals { public static String VERSION; public static String BUILD; public static String TIMESTAMP; - public static double log2 = Math.log(2); final public static boolean IS_WINDOWS = @@ -197,4 +194,7 @@ public static boolean checkJavaVersion(String minVersion) { return curVersion.compareTo(minVersion) >= 0; } + public static double log2(final double value) { + return Math.log(value) / Math.log(2); + } } diff --git a/src/main/java/org/broad/igv/data/seg/FreqData.java b/src/main/java/org/broad/igv/data/seg/FreqData.java index 0f122a9142..e3473af0bf 100644 --- a/src/main/java/org/broad/igv/data/seg/FreqData.java +++ b/src/main/java/org/broad/igv/data/seg/FreqData.java @@ -120,7 +120,7 @@ public void compute(float ampThreshold, float delThreshold) { for (LocusScore seg : segments) { final float segScore = logNormalized ? seg.getScore() : - (float) (Math.log(seg.getScore() / 2) / Globals.log2); + (float) (Globals.log2(seg.getScore() / 2)); if (segScore > ampThreshold || segScore < delThreshold) { diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrack.java b/src/main/java/org/broad/igv/sam/AlignmentTrack.java index 0a0b4570b2..3f306adf42 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrack.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrack.java @@ -506,10 +506,15 @@ public void load(ReferenceFrame referenceFrame) { dataManager.load(referenceFrame, renderOptions, true); } + @Override + public int getVisibilityWindow() { + return (int) dataManager.getVisibilityWindow(); + } + public void render(RenderContext context, Rectangle rect) { int viewWindowSize = context.getReferenceFrame().getCurrentRange().getLength(); - if (viewWindowSize > dataManager.getVisibilityWindow()) { + if (viewWindowSize > getVisibilityWindow()) { Rectangle visibleRect = context.getVisibleRect().intersection(rect); Graphics2D g2 = context.getGraphic2DForColor(Color.gray); GraphicUtils.drawCenteredText("Zoom in to see alignments.", visibleRect, g2); diff --git a/src/main/java/org/broad/igv/sam/CoverageTrack.java b/src/main/java/org/broad/igv/sam/CoverageTrack.java index 5ff9f89d7e..e271bd767f 100644 --- a/src/main/java/org/broad/igv/sam/CoverageTrack.java +++ b/src/main/java/org/broad/igv/sam/CoverageTrack.java @@ -214,10 +214,15 @@ public void unload() { setVisible(false); } + @Override + public int getVisibilityWindow() { + return (int) dataManager.getVisibilityWindow(); + } + public void render(RenderContext context, Rectangle rect) { int viewWindowSize = context.getReferenceFrame().getCurrentRange().getLength(); - if (viewWindowSize > dataManager.getVisibilityWindow() && dataSource == null) { + if (viewWindowSize > getVisibilityWindow() && dataSource == null) { Rectangle visibleRect = context.getVisibleRect().intersection(rect); Graphics2D g = context.getGraphic2DForColor(Color.gray); GraphicUtils.drawCenteredText("Zoom in to see coverage.", visibleRect, g); diff --git a/src/main/java/org/broad/igv/sashimi/SashimiJunctionRenderer.java b/src/main/java/org/broad/igv/sashimi/SashimiJunctionRenderer.java index b294da01f5..97694b1909 100644 --- a/src/main/java/org/broad/igv/sashimi/SashimiJunctionRenderer.java +++ b/src/main/java/org/broad/igv/sashimi/SashimiJunctionRenderer.java @@ -362,7 +362,7 @@ protected void drawFeature(int pixelYStartOffset, int pixelYEndOffset, int length = pixelJunctionEnd - pixelJunctionStart; int minArcHeight = (trackRectangle.height - 1) / 8; //We adjust the height slightly by length of junction, just so arcs don't overlap as much - int arcHeight = minArcHeight + (int) (Math.log(length) / Globals.log2); + int arcHeight = minArcHeight + (int) (Globals.log2(length)); int minY = (int) trackRectangle.getCenterY() + Math.min(pixelYStartOffset - arcHeight, pixelYEndOffset - arcHeight); //Check if arc goes too high. All arcs going below have the same height, diff --git a/src/main/java/org/broad/igv/tools/converters/ExpressionFormatter.java b/src/main/java/org/broad/igv/tools/converters/ExpressionFormatter.java index b807887bf9..6b90a59aea 100644 --- a/src/main/java/org/broad/igv/tools/converters/ExpressionFormatter.java +++ b/src/main/java/org/broad/igv/tools/converters/ExpressionFormatter.java @@ -192,7 +192,7 @@ class DataRow { throw new RuntimeException("Negative value detected in input file: " + line); } - double v = Math.log(data[dataIdx]) / Globals.log2; + double v = Globals.log2(data[dataIdx]); scaledData[dataIdx] = v; nonNullData[nNonNull] = v; nNonNull++; diff --git a/src/main/java/org/broad/igv/track/AbstractTrack.java b/src/main/java/org/broad/igv/track/AbstractTrack.java index fda4333ca1..85893be5ab 100644 --- a/src/main/java/org/broad/igv/track/AbstractTrack.java +++ b/src/main/java/org/broad/igv/track/AbstractTrack.java @@ -806,7 +806,7 @@ public float logScaleData(float dataY) { double centerValue = (getTrackType() == TrackType.ALLELE_SPECIFIC_COPY_NUMBER) ? 1.0 : 2.0; - return (float) (Math.log(Math.max(Float.MIN_VALUE, dataY) / centerValue) / Globals.log2); + return (float) (Globals.log2(Math.max(Float.MIN_VALUE, dataY) / centerValue)); } else { return dataY; } diff --git a/src/main/java/org/broad/igv/ui/panel/ReferenceFrame.java b/src/main/java/org/broad/igv/ui/panel/ReferenceFrame.java index 6e261a778d..2758372c7a 100644 --- a/src/main/java/org/broad/igv/ui/panel/ReferenceFrame.java +++ b/src/main/java/org/broad/igv/ui/panel/ReferenceFrame.java @@ -498,7 +498,7 @@ private boolean shouldChangeChromosome(String newChrName) { protected void calculateMaxZoom() { this.maxZoom = Globals.CHR_ALL.equals(this.chrName) ? 0 : - (int) Math.ceil(Math.log(getChromosomeLength() / minBP) / Globals.log2); + (int) Math.ceil(Globals.log2(getChromosomeLength() / minBP)); } public String getChrName() { @@ -714,8 +714,9 @@ private void beforeScaleZoom(Locus locus) { * @param end * @return */ - private int calculateZoom(double start, double end) { - return (int) Math.round((Math.log((getChromosomeLength() / (end - start)) * (((double) widthInPixels) / binsPerTile)) / Globals.log2)); + public int calculateZoom(double start, double end) { + final double windowLength = Math.min(end - start, getChromosomeLength()); + return (int) Math.round(Globals.log2((getChromosomeLength() / windowLength) * (((double) widthInPixels) / binsPerTile))); } diff --git a/src/main/java/org/broad/igv/ui/panel/ZoomSliderPanel.java b/src/main/java/org/broad/igv/ui/panel/ZoomSliderPanel.java index b3954207e0..c78082b958 100644 --- a/src/main/java/org/broad/igv/ui/panel/ZoomSliderPanel.java +++ b/src/main/java/org/broad/igv/ui/panel/ZoomSliderPanel.java @@ -33,13 +33,20 @@ */ package org.broad.igv.ui.panel; +import org.broad.igv.track.Track; +import org.broad.igv.ui.IGV; import org.broad.igv.ui.util.IGVMouseInputAdapter; import org.broad.igv.ui.util.IconFactory; import javax.swing.*; import javax.swing.event.MouseInputAdapter; + +import java.util.List; import java.awt.*; import java.awt.event.MouseEvent; +import java.util.SortedSet; +import java.util.TreeSet; +import java.util.stream.Collectors; /** * @author jrobinso @@ -47,6 +54,7 @@ public class ZoomSliderPanel extends JPanel { private static final Color TRANSPARENT_GRAY = new Color(200, 200, 200, 150); + private static final Color TRANSPARENT_BLUE = new Color(27, 96, 246, 25); static Color TICK_GRAY = new Color(90, 90, 90); static Color TICK_BLUE = new Color(25, 50, 200); @@ -103,7 +111,6 @@ private void updateTickCount() { numZoomLevels = tmp; zoomLevelRects = new Rectangle[numZoomLevels]; } - } @@ -193,9 +200,33 @@ protected void paintHorizontal(Graphics g) { //g.drawImage(slider, x + 1, y, null); } } + + paintVisibilityThresholds(transGraphics); transGraphics.dispose(); } + //Adds an indicator of which zoom level will display reads/features + private void paintVisibilityThresholds(final Graphics2D transGraphics) { + if(numZoomLevels > 1) { + List visibilityThresholds = IGV.getInstance().getAllTracks().stream() + .map(Track::getVisibilityWindow) + .filter(i -> i > 0) + .sorted() + .distinct() + .map(threshold -> this.getReferenceFrame().calculateZoom(0, threshold)) + .collect(Collectors.toList()); + + transGraphics.setColor(TRANSPARENT_BLUE); + Rectangle maxZoom = zoomLevelRects[zoomLevelRects.length - 1]; + for (Integer window : visibilityThresholds) { + final Rectangle currentLevel = zoomLevelRects[window]; + Rectangle windowBox = new Rectangle(currentLevel.x, currentLevel.y, + maxZoom.x + maxZoom.width - currentLevel.x, currentLevel.height); + transGraphics.fill(windowBox); + } + } + } + void setZoom(MouseEvent e) { if (zoomPlusRect.contains(e.getX(), e.getY())) { diff --git a/test/sessions/base_mods/HIFI_basemodifictions.xml b/test/sessions/base_mods/HIFI_basemodifictions.xml index b8e02a156c..2c581bbe7f 100644 --- a/test/sessions/base_mods/HIFI_basemodifictions.xml +++ b/test/sessions/base_mods/HIFI_basemodifictions.xml @@ -1,22 +1,22 @@ - + - + - + - + - + - + - + From 441d13876911d2852d41a4a0f5844a8e78320b7d Mon Sep 17 00:00:00 2001 From: tomkp75 Date: Tue, 21 Feb 2023 12:40:43 -0500 Subject: [PATCH 034/116] Adding Access-Control-Allow-Headers (#1284) * Adding Access-Control-Allow-Headers Adding Access-Control-Allow-Headers to sendHTTPOptionsResponse for options preflight to pass * Print CRLF vs println (cross-platform) Print CRLF instead of using println for this to work on all platforms --- src/main/java/org/broad/igv/batch/CommandListener.java | 5 ++++- 1 file changed, 4 insertions(+), 1 deletion(-) diff --git a/src/main/java/org/broad/igv/batch/CommandListener.java b/src/main/java/org/broad/igv/batch/CommandListener.java index df99b658a2..ff0992fa46 100755 --- a/src/main/java/org/broad/igv/batch/CommandListener.java +++ b/src/main/java/org/broad/igv/batch/CommandListener.java @@ -312,7 +312,8 @@ private void closeSockets() { private static final String CONNECTION_CLOSE = "Connection: close"; private static final String NO_CACHE = "Cache-Control: no-cache, no-store"; private static final String ACCESS_CONTROL_ALLOW_ORIGIN = "Access-Control-Allow-Origin: *"; - + private static final String ACCESS_CONTROL_ALLOW_HEADERS = "Access-Control-Allow-Headers: access-control-allow-origin"; + private void sendTextResponse(PrintWriter out, String result) { sendHTTPResponse(out, result, "text/html", "GET"); } @@ -348,6 +349,8 @@ private void sendHTTPOptionsResponse(PrintWriter out) { out.print(CRLF); out.print(ACCESS_CONTROL_ALLOW_ORIGIN); out.print(CRLF); + out.print(ACCESS_CONTROL_ALLOW_HEADERS); + out.print(CRLF); out.println("Access-Control-Allow-Methods: HEAD, GET, OPTIONS"); out.print(CRLF); From 33e754dd430d351a8c8316f8922ccec3f1a8d856 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Tue, 21 Feb 2023 12:00:28 -0800 Subject: [PATCH 035/116] Reduce needless use of reflection to instantiate renderer objects --- .../igv/feature/EmblFeatureTableParser.java | 2 +- .../igv/feature/dranger/DRangerParser.java | 2 +- .../broad/igv/sam/SpliceJunctionTrack.java | 7 ++++-- .../org/broad/igv/sashimi/SashimiPlot.java | 2 +- .../org/broad/igv/track/AbstractTrack.java | 2 +- .../igv/track/CombinedDataSourceDialog.java | 2 +- .../java/org/broad/igv/track/DataTrack.java | 2 +- .../org/broad/igv/track/FeatureTrack.java | 2 +- .../igv/track/SelectableFeatureTrack.java | 2 +- .../java/org/broad/igv/track/TrackLoader.java | 14 +++++------ .../org/broad/igv/track/TrackMenuUtils.java | 25 ++----------------- 11 files changed, 22 insertions(+), 40 deletions(-) diff --git a/src/main/java/org/broad/igv/feature/EmblFeatureTableParser.java b/src/main/java/org/broad/igv/feature/EmblFeatureTableParser.java index 7d5d2ecd9a..ef5e202f34 100644 --- a/src/main/java/org/broad/igv/feature/EmblFeatureTableParser.java +++ b/src/main/java/org/broad/igv/feature/EmblFeatureTableParser.java @@ -78,7 +78,7 @@ public List loadTracks(ResourceLocator locator, Genome genome) { track.setName(locator.getTrackName()); track.setMinimumHeight(35); track.setHeight(45); - track.setRendererClass(GeneTrackRenderer.class); + track.setRenderer(new GeneTrackRenderer()); List newTracks = new ArrayList(); diff --git a/src/main/java/org/broad/igv/feature/dranger/DRangerParser.java b/src/main/java/org/broad/igv/feature/dranger/DRangerParser.java index 25a9216ca9..bd5e451dee 100644 --- a/src/main/java/org/broad/igv/feature/dranger/DRangerParser.java +++ b/src/main/java/org/broad/igv/feature/dranger/DRangerParser.java @@ -153,7 +153,7 @@ public List loadTracks(ResourceLocator locator, Genome genome) { FeatureTrack track = new FeatureTrack(locator, new FeatureCollectionSource(features, genome)); track.setName(locator.getTrackName()); - track.setRendererClass(DRangerRenderer.class); + track.setRenderer(new DRangerRenderer()); tracks.add(track); return tracks; diff --git a/src/main/java/org/broad/igv/sam/SpliceJunctionTrack.java b/src/main/java/org/broad/igv/sam/SpliceJunctionTrack.java index 325d9e2c23..d3078f25fd 100644 --- a/src/main/java/org/broad/igv/sam/SpliceJunctionTrack.java +++ b/src/main/java/org/broad/igv/sam/SpliceJunctionTrack.java @@ -81,7 +81,7 @@ public SpliceJunctionTrack(ResourceLocator locator, String name, super(locator, locator.getPath() + "_junctions", name); super.setDataRange(new DataRange(0, 0, 60)); - setRendererClass(SpliceJunctionRenderer.class); + this.renderer = new SpliceJunctionRenderer(); if (dataManager != null) { dataManager.unsubscribe(this); } @@ -92,6 +92,7 @@ public SpliceJunctionTrack(ResourceLocator locator, String name, } public SpliceJunctionTrack() { + this.renderer = new SpliceJunctionRenderer(); } @Override @@ -384,7 +385,9 @@ public void unmarshalXML(Element element, Integer version) { } if(element.hasAttribute("maxdepth")) { - ((SpliceJunctionRenderer) renderer).setMaxDepth((int) Float.parseFloat(element.getAttribute("maxdepth"))); + if(renderer != null && renderer instanceof SpliceJunctionRenderer) { + ((SpliceJunctionRenderer) renderer).setMaxDepth((int) Float.parseFloat(element.getAttribute("maxdepth"))); + } } } diff --git a/src/main/java/org/broad/igv/sashimi/SashimiPlot.java b/src/main/java/org/broad/igv/sashimi/SashimiPlot.java index dd27566ec7..78e7f1b33e 100644 --- a/src/main/java/org/broad/igv/sashimi/SashimiPlot.java +++ b/src/main/java/org/broad/igv/sashimi/SashimiPlot.java @@ -125,7 +125,7 @@ public SashimiPlot(ReferenceFrame iframe, Collection a // Override expand/collpase setting -- expanded sashimi plots make no sense spliceJunctionTrack.setDisplayMode(Track.DisplayMode.COLLAPSED); - spliceJunctionTrack.setRendererClass(SashimiJunctionRenderer.class); + spliceJunctionTrack.setRenderer(new SashimiJunctionRenderer()); Color color = plotColors.get(colorInd); colorInd = (colorInd + 1) % plotColors.size(); diff --git a/src/main/java/org/broad/igv/track/AbstractTrack.java b/src/main/java/org/broad/igv/track/AbstractTrack.java index 85893be5ab..206e8dbc24 100644 --- a/src/main/java/org/broad/igv/track/AbstractTrack.java +++ b/src/main/java/org/broad/igv/track/AbstractTrack.java @@ -934,7 +934,7 @@ public void unload() { } - protected void setRenderer(Renderer renderer) { + public void setRenderer(Renderer renderer) { //Here as setter for corresponding getter, subclasses should override } diff --git a/src/main/java/org/broad/igv/track/CombinedDataSourceDialog.java b/src/main/java/org/broad/igv/track/CombinedDataSourceDialog.java index de11206153..1f0697b04a 100644 --- a/src/main/java/org/broad/igv/track/CombinedDataSourceDialog.java +++ b/src/main/java/org/broad/igv/track/CombinedDataSourceDialog.java @@ -100,7 +100,7 @@ private void okButtonActionPerformed(ActionEvent e) { CombinedDataTrack newTrack = new CombinedDataTrack(dataSource, id, name); - TrackMenuUtils.changeRenderer(Arrays.asList(newTrack), track0.getRenderer().getClass()); + TrackMenuUtils.changeRendererClass(Arrays.asList(newTrack), track0.getRenderer().getClass()); newTrack.setDataRange(track0.getDataRange()); newTrack.setColorScale(track0.getColorScale()); IGV.getInstance().addTracks(Arrays.asList(newTrack), PanelName.DATA_PANEL); diff --git a/src/main/java/org/broad/igv/track/DataTrack.java b/src/main/java/org/broad/igv/track/DataTrack.java index b78be82058..2340e91de1 100644 --- a/src/main/java/org/broad/igv/track/DataTrack.java +++ b/src/main/java/org/broad/igv/track/DataTrack.java @@ -251,7 +251,7 @@ public void setRendererClass(Class rc) { } @Override - protected void setRenderer(Renderer renderer) { + public void setRenderer(Renderer renderer) { this.renderer = (DataRenderer) renderer; } diff --git a/src/main/java/org/broad/igv/track/FeatureTrack.java b/src/main/java/org/broad/igv/track/FeatureTrack.java index cec5aa9e5a..35865fbfa8 100644 --- a/src/main/java/org/broad/igv/track/FeatureTrack.java +++ b/src/main/java/org/broad/igv/track/FeatureTrack.java @@ -363,7 +363,7 @@ public float getRegionScore(String chr, int start, int end, int zoom, RegionScor public Renderer getRenderer() { if (renderer == null) { - setRendererClass(IGVFeatureRenderer.class); + setRenderer(new IGVFeatureRenderer()); } return renderer; } diff --git a/src/main/java/org/broad/igv/track/SelectableFeatureTrack.java b/src/main/java/org/broad/igv/track/SelectableFeatureTrack.java index 9566897a3f..6ef69b93dd 100644 --- a/src/main/java/org/broad/igv/track/SelectableFeatureTrack.java +++ b/src/main/java/org/broad/igv/track/SelectableFeatureTrack.java @@ -50,7 +50,7 @@ public SelectableFeatureTrack() { public SelectableFeatureTrack(FeatureTrack geneTrack) { super(geneTrack); - this.setRendererClass(SelectableFeatureRenderer.class); + this.setRenderer(new SelectableFeatureRenderer()); } @Override diff --git a/src/main/java/org/broad/igv/track/TrackLoader.java b/src/main/java/org/broad/igv/track/TrackLoader.java index a4ca8716f3..856eff84bc 100644 --- a/src/main/java/org/broad/igv/track/TrackLoader.java +++ b/src/main/java/org/broad/igv/track/TrackLoader.java @@ -329,7 +329,7 @@ private void loadSMAPFile(ResourceLocator locator, List newTracks, Genome List features = SMAPParser.parseFeatures(locator, genome); FeatureCollectionSource src = new FeatureCollectionSource(features, genome); FeatureTrack track = new FeatureTrack(locator, locator.getName(), src); - track.setRendererClass(SMAPRenderer.class); + track.setRenderer(new SMAPRenderer()); track.setDisplayMode(Track.DisplayMode.EXPANDED); newTracks.add(track); } @@ -526,7 +526,7 @@ private void loadDSIFile(ResourceLocator locator, List newTracks, Genome t.setTrackLine(tp.getTrackLine()); } - t.setRendererClass(DSIRenderer.class); + t.setRenderer(new DSIRenderer()); newTracks.add(t); } @@ -590,8 +590,8 @@ private void loadGctFile(ResourceLocator locator, List newTracks, Genome for (String trackName : ds.getTrackNames()) { DatasetDataSource dataSource = new DatasetDataSource(trackName, ds, genome); String trackId = path + "_" + trackName; - Track track = new DataSourceTrack(locator, trackId, trackName, dataSource); - track.setRendererClass(HeatmapRenderer.class); + DataSourceTrack track = new DataSourceTrack(locator, trackId, trackName, dataSource); + track.setRenderer(new HeatmapRenderer()); track.setProperties(trackProperties); newTracks.add(track); } @@ -644,7 +644,7 @@ private void loadIGVFile(ResourceLocator locator, List newTracks, Genome track.setProperties(trackProperties); if (type == TrackType.ALLELE_FREQUENCY) { - track.setRendererClass(PointsRenderer.class); + track.setRenderer(new PointsRenderer()); track.setHeight(40); } newTracks.add(track); @@ -1019,7 +1019,7 @@ private void loadMutFile(ResourceLocator locator, List newTracks, Genome List mutationTracks = loader.loadMutationTracks(locator, genome); for (FeatureTrack track : mutationTracks) { track.setTrackType(TrackType.MUTATION); - track.setRendererClass(MutationRenderer.class); + track.setRenderer(new MutationRenderer()); newTracks.add(track); } } @@ -1077,7 +1077,7 @@ private void loadSegTrack(ResourceLocator locator, List newTracks, Genome String trackId = path + "_" + trackName; SegmentedDataSource dataSource = new SegmentedDataSource(trackName, ds); DataSourceTrack track = new DataSourceTrack(locator, trackId, trackName, dataSource); - track.setRendererClass(HeatmapRenderer.class); + track.setRenderer(new HeatmapRenderer()); track.setTrackType(ds.getType()); if (props != null) { diff --git a/src/main/java/org/broad/igv/track/TrackMenuUtils.java b/src/main/java/org/broad/igv/track/TrackMenuUtils.java index 14eae88b14..01a8f3c138 100644 --- a/src/main/java/org/broad/igv/track/TrackMenuUtils.java +++ b/src/main/java/org/broad/igv/track/TrackMenuUtils.java @@ -258,7 +258,7 @@ public static void addDataItems(JPopupMenu menu, final Collection tracks, } item.addActionListener(new ActionListener() { public void actionPerformed(ActionEvent evt) { - changeRenderer(tracks, rendererClass); + changeRendererClass(tracks, rendererClass); } }); menu.add(item); @@ -386,27 +386,6 @@ public void actionPerformed(ActionEvent evt) { } - private static void addCombinedDataTrack(List dataTracks, CombinedDataSource.Operation op) { - String text = ""; - switch (op) { - case ADD: - text = "Sum"; - break; - case SUBTRACT: - text = "Difference"; - break; - } - DataTrack track0 = dataTracks.get(0); - DataTrack track1 = dataTracks.get(1); - CombinedDataSource source = new CombinedDataSource(track0, track1, op); - - DataSourceTrack newTrack = new DataSourceTrack(null, track0.getId() + track1.getId() + text, text, source); - changeRenderer(Arrays.asList(newTrack), track0.getRenderer().getClass()); - newTrack.setDataRange(track0.getDataRange()); - newTrack.setColorScale(track0.getColorScale()); - IGV.getInstance().addTracks(Arrays.asList(newTrack), PanelName.DATA_PANEL); - } - /** * Return popup menu with items applicable to feature tracks * @@ -1019,7 +998,7 @@ public static void removeTracksAction(final Collection selectedTracks) { } - public static void changeRenderer(final Collection selectedTracks, Class rendererClass) { + public static void changeRendererClass(final Collection selectedTracks, Class rendererClass) { for (Track track : selectedTracks) { track.setRendererClass(rendererClass); } From ac507b2819adfcdcf5539479005e82be42970c66 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Tue, 21 Feb 2023 12:01:32 -0800 Subject: [PATCH 036/116] fix unit tests --- .../genome/fasta/FastaSequenceParserTest.java | 18 ++-- .../sam/reader/CGIAlignmentReaderTest.java | 102 ------------------ .../java/org/broad/igv/tdf/TDFReaderTest.java | 6 +- .../broad/igv/tools/CoverageCounterTest.java | 20 ---- .../org/broad/igv/util/HttpUtilsTest.java | 7 +- 5 files changed, 14 insertions(+), 139 deletions(-) delete mode 100644 src/test/java/org/broad/igv/sam/reader/CGIAlignmentReaderTest.java diff --git a/src/test/java/org/broad/igv/feature/genome/fasta/FastaSequenceParserTest.java b/src/test/java/org/broad/igv/feature/genome/fasta/FastaSequenceParserTest.java index 7c7f62a19f..39068fb93d 100644 --- a/src/test/java/org/broad/igv/feature/genome/fasta/FastaSequenceParserTest.java +++ b/src/test/java/org/broad/igv/feature/genome/fasta/FastaSequenceParserTest.java @@ -45,7 +45,7 @@ public class FastaSequenceParserTest { @BeforeClass public static void setup() throws IOException { - String path = "http://data.broadinstitute.org/igvdata/test/fasta/ci2_test.fa"; + String path = "https://igv.genepattern.org/test/fasta/chr22.fa"; Map sequenceMap = FastaSequenceParser.parseFasta(path); fastaSequence = new InMemorySequence(sequenceMap); } @@ -54,14 +54,14 @@ public static void setup() throws IOException { public void testReadSequence() throws Exception { - String chr = "chr02q"; - int start = 3531385; - int end = 3531425; + String chr = "chr22"; + int start = 31084127; + int end = 31084167; - String expectedSequence = "TAATTTTTACGTCTTATTTAAACACATATAATGAATAGGT"; + String expectedSequence = "GCCACCATGCCTGGCTAGTTTTTTGTATTTTTAGTAGAGA"; byte[] seq = fastaSequence.getSequence(chr, start, end, true); - String seqString = new String(seq); + String seqString = new String(seq).toUpperCase(); assertEquals(expectedSequence, seqString); } @@ -69,14 +69,14 @@ public void testReadSequence() throws Exception { @Test public void testReadEnd() throws Exception { - String chr = "chr02q"; - int chrLen = 8059593; + String chr = "chr22"; + int chrLen = 51304566; int start = chrLen - 10; int end = chrLen + 10; byte[] bytes = fastaSequence.getSequence(chr, start, end, true); assertEquals(10, bytes.length); - byte[] expectedSequence = "TTTTTCCCAG".getBytes(); + byte[] expectedSequence = "NNNNNNNNNN".getBytes(); for (int i = 0; i < 10; i++) { assertEquals(expectedSequence[i], bytes[i]); diff --git a/src/test/java/org/broad/igv/sam/reader/CGIAlignmentReaderTest.java b/src/test/java/org/broad/igv/sam/reader/CGIAlignmentReaderTest.java deleted file mode 100644 index f1ba576730..0000000000 --- a/src/test/java/org/broad/igv/sam/reader/CGIAlignmentReaderTest.java +++ /dev/null @@ -1,102 +0,0 @@ -/* - * The MIT License (MIT) - * - * Copyright (c) 2007-2015 Broad Institute - * - * Permission is hereby granted, free of charge, to any person obtaining a copy - * of this software and associated documentation files (the "Software"), to deal - * in the Software without restriction, including without limitation the rights - * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell - * copies of the Software, and to permit persons to whom the Software is - * furnished to do so, subject to the following conditions: - * - * The above copyright notice and this permission notice shall be included in - * all copies or substantial portions of the Software. - * - * - * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR - * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, - * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE - * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER - * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, - * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN - * THE SOFTWARE. - */ - -package org.broad.igv.sam.reader; - -import htsjdk.samtools.util.CloseableIterator; -import org.broad.igv.Globals; -import org.broad.igv.sam.Alignment; -import org.junit.BeforeClass; -import org.junit.Test; - -import java.net.MalformedURLException; -import java.util.Iterator; - -import static org.junit.Assert.assertEquals; -import static org.junit.Assert.assertTrue; - -/** - * @author Jim Robinson - * @date 1/10/12 - */ -public class CGIAlignmentReaderTest { - - @BeforeClass - public static void setup() { - Globals.setHeadless(true); - } - - @Test - public void testURLs() throws MalformedURLException { - - String testURL = "http://somehost/cramtools/cgi-bin/query.cgi?program=downsample_bam.pl&downsampled_coverage=10&file=input.sam/"; - String queryPath = "query.cgi?"; - String headerPath = "samHeader.cgi?"; - String seqNamePath = "getSequenceNames.cgi?"; - - CGIAlignmentReader reader = new CGIAlignmentReader(testURL); - - assertEquals(testURL, reader.getQueryURL()); - assertEquals(testURL.replace(queryPath, headerPath), reader.getHeaderURL()); - assertEquals(testURL .replace(queryPath, seqNamePath), reader.getSequenceNamesURL()); - - } - - @Test - public void testURLsWithPort() throws MalformedURLException { - - String testURL = "http://somehost:8080/cramtools/cgi-bin/query.cgi?program=downsample_bam.pl&downsampled_coverage=10&file=input.sam/"; - String queryPath = "query.cgi?"; - String headerPath = "samHeader.cgi?"; - String seqNamePath = "getSequenceNames.cgi?"; - - CGIAlignmentReader reader = new CGIAlignmentReader(testURL); - - assertEquals(testURL, reader.getQueryURL()); - assertEquals(testURL.replace(queryPath, headerPath), reader.getHeaderURL()); - assertEquals(testURL .replace(queryPath, seqNamePath), reader.getSequenceNamesURL()); - - } - - - - // The following test is disabled until we can write our own CGI scripts to test against. - //@Test -// public void testQuery() throws Exception { -// -// String testURL = "http://somehost/query.cgi?file=input.sam"; -// -// CGIAlignmentReader reader = new CGIAlignmentReader(testURL); -// -// // Assert that we get at least 1 record back. -// CloseableIterator iter = reader.iterator(); -// assertTrue(iter.hasNext()); -// iter.close(); -// -// -// } - - -} diff --git a/src/test/java/org/broad/igv/tdf/TDFReaderTest.java b/src/test/java/org/broad/igv/tdf/TDFReaderTest.java index e0d8fde632..0fb17ca215 100644 --- a/src/test/java/org/broad/igv/tdf/TDFReaderTest.java +++ b/src/test/java/org/broad/igv/tdf/TDFReaderTest.java @@ -43,12 +43,12 @@ public class TDFReaderTest { @Test public void testReader() throws Exception { - String url = "http://data.broadinstitute.org/igvdata/encode/hg18/broadHistone/SignalK562H3k4me3.tdf"; + String url = "http://data.broadinstitute.org/igvdata/tutorials/hg18/wgEncodeBroadChipSeqSignalK562H3k4me3.wig.gz.tdf"; TDFReader reader = new TDFReader(new ResourceLocator(url)); int version = reader.getVersion(); - assertEquals(3, version); + assertEquals(4, version); assertTrue(reader.compressed); String [] trackNames = reader.getTrackNames(); @@ -56,7 +56,7 @@ public void testReader() throws Exception { assertEquals(1, nTracks); String trackName = trackNames[0]; - assertEquals(trackName, "SignalK562H3k4me3.wig.gz"); + assertEquals(trackName, "wgEncodeBroadChipSeqSignalK562H3k4me3.wig.gz"); Set chrNames = reader.getChromosomeNames(); int nChromosomes = chrNames.size(); diff --git a/src/test/java/org/broad/igv/tools/CoverageCounterTest.java b/src/test/java/org/broad/igv/tools/CoverageCounterTest.java index e92b74af4f..8089443cc7 100644 --- a/src/test/java/org/broad/igv/tools/CoverageCounterTest.java +++ b/src/test/java/org/broad/igv/tools/CoverageCounterTest.java @@ -228,26 +228,6 @@ public void testColumnCounts() throws Exception { } - @Test - public void testIncludeDuplicatesFlag() throws IOException { - String bamURL = "http://data.broadinstitute.org/igvdata/BodyMap/hg18/Merged/HBM.adipose.bam.sorted.bam"; - int options = CoverageCounter.INCLUDE_DUPS; - String queryString = "chr1:153425249-153425249"; - int windowSize = 1; - File wigFile = null; - Genome genome = null; - - TestDataConsumer dc = new TestDataConsumer(); - - CoverageCounter cc = new CoverageCounter(bamURL, dc, windowSize, 0, wigFile, genome, queryString, 0, options); - - cc.parse(); - - String totalCount = dc.attributes.get("totalCount"); - - assertEquals("22", totalCount); - - } static class TestDataConsumer implements DataConsumer { diff --git a/src/test/java/org/broad/igv/util/HttpUtilsTest.java b/src/test/java/org/broad/igv/util/HttpUtilsTest.java index 52a9da7611..270936c454 100644 --- a/src/test/java/org/broad/igv/util/HttpUtilsTest.java +++ b/src/test/java/org/broad/igv/util/HttpUtilsTest.java @@ -47,10 +47,7 @@ public class HttpUtilsTest extends AbstractHeadlessTest { - static String broadURLString = "http://data.broadinstitute.org/igvdata/annotations/seq/hg19/chr1.txt"; - static String genericURLString = "http://hgdownload.cse.ucsc.edu/goldenPath/hg19/chromosomes/chr1.fa.gz"; - - static String noRangeHeaderSupportString = "http://www.ncbi.nlm.nih.gov/geo/download/?acc=GSM714693&format=file&file=GSM714693%5Fhg19%5FwgEncodeGisDnaPetK562F1kAln%2Ebam"; + static String broadURLString = "https://igv-genepattern-org.s3.amazonaws.com/test/fasta/chr22.fa"; @Test public void testGetContentLength() throws IOException { @@ -59,7 +56,7 @@ public void testGetContentLength() throws IOException { try { conn = (HttpURLConnection) (HttpUtils.createURL(broadURLString)).openConnection(); String contentLength = conn.getHeaderField("Content-length"); - assertEquals("249250621", contentLength); + assertEquals("52330665", contentLength); } finally { From b5136e64156cccc986aa4dce44b13c8d304dc4e2 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 23 Mar 2023 12:36:25 -0700 Subject: [PATCH 037/116] Do some minimal validation of MM and ML tags. Fixes #1305 --- src/main/java/org/broad/igv/sam/SAMAlignment.java | 15 ++++++++++----- 1 file changed, 10 insertions(+), 5 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/SAMAlignment.java b/src/main/java/org/broad/igv/sam/SAMAlignment.java index 884fd4ce1e..432faaeb4d 100644 --- a/src/main/java/org/broad/igv/sam/SAMAlignment.java +++ b/src/main/java/org/broad/igv/sam/SAMAlignment.java @@ -330,12 +330,17 @@ public List getBaseModificationSets() { Object mm = record.hasAttribute("Mm") ? record.getAttribute("Mm") : record.getAttribute("MM"); byte[] ml = (byte[]) (record.hasAttribute("Ml") ? record.getAttribute("Ml") : record.getAttribute("ML")); - byte[] sequence = record.getReadBases(); - if (mm.toString().length() == 0) { // TODO -- more extensive validation? - baseModificationSets = Collections.EMPTY_LIST; - } else { - baseModificationSets = BaseModificationUtils.getBaseModificationSets(mm.toString(), ml, sequence, isNegativeStrand()); + // Minimal tag validation + if(mm instanceof String && (ml == null || ml instanceof byte [])) { + + byte[] sequence = record.getReadBases(); + + if (mm.toString().length() == 0) { // TODO -- more extensive validation? + baseModificationSets = Collections.EMPTY_LIST; + } else { + baseModificationSets = BaseModificationUtils.getBaseModificationSets((String) mm, ml, sequence, isNegativeStrand()); + } } } return baseModificationSets; From 8527dfaa2a14d3d4a464a45da9fded0081961029 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 30 Mar 2023 16:26:35 -0700 Subject: [PATCH 038/116] Show details for inter-chromosomal bedpe/interact features. Fixes #1302 --- src/main/java/org/broad/igv/bedpe/InteractionTrack.java | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/src/main/java/org/broad/igv/bedpe/InteractionTrack.java b/src/main/java/org/broad/igv/bedpe/InteractionTrack.java index 3891d0c682..890721cb6e 100644 --- a/src/main/java/org/broad/igv/bedpe/InteractionTrack.java +++ b/src/main/java/org/broad/igv/bedpe/InteractionTrack.java @@ -379,7 +379,9 @@ private void setGraphType(GraphType value) { @Override public String getValueStringAt(String chr, double position, int mouseX, int mouseY, ReferenceFrame frame) { - List candidates = getFeaturesOverlapping(frame.getChrName(), (int) position, (int) position + 1); + // Expand range a little bit -- this should be done in pixels + double tolerance = frame.getScale() * 3; + List candidates = getFeaturesOverlapping(frame.getChrName(), (int) position - tolerance, (int) position + 1 + tolerance); // Sort candidate features smallest to largest Comparator sorter = graphType == GraphType.PROPORTIONAL_ARC ? @@ -402,7 +404,7 @@ public String getValueStringAt(String chr, double position, int mouseX, int mous for (BedPE f : candidates) { BedPEShape s = f.getShape(); - if (s != null && s.contains(mouseX, mouseY)) { + if (s == null || s.contains(mouseX, mouseY)) { return f.getValueString(); } } From d1b96f6cc27db7b689f5037a55af361179ca3e3a Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 30 Mar 2023 16:27:06 -0700 Subject: [PATCH 039/116] update url mapping --- src/main/java/org/broad/igv/util/HttpUtils.java | 2 ++ 1 file changed, 2 insertions(+) diff --git a/src/main/java/org/broad/igv/util/HttpUtils.java b/src/main/java/org/broad/igv/util/HttpUtils.java index bfa592479f..60250c434e 100644 --- a/src/main/java/org/broad/igv/util/HttpUtils.java +++ b/src/main/java/org/broad/igv/util/HttpUtils.java @@ -226,6 +226,8 @@ public static String mapURL(String urlString) throws MalformedURLException { urlString = urlString.replace("//www.dropbox.com", "//dl.dropboxusercontent.com"); } else if (host.equals("drive.google.com")) { urlString = GoogleUtils.driveDownloadURL(urlString); + } else if (host.equals("igv.genepattern.org")) { + urlString = urlString.replace("//igv.genepattern.org", "//igv-genepattern-org.s3.amazonaws.com"); } // data.broadinstitute.org requires https From bf7427f3a2cdf20f6fb30422d18667e8ed91345f Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 5 Apr 2023 09:57:19 -0700 Subject: [PATCH 040/116] Provisionally modify oAuth provisioning URLs with HttpUtils.mapURL (e.g. converting dropbox share URL to download URL) --- src/main/java/org/broad/igv/oauth/OAuthUtils.java | 3 +++ 1 file changed, 3 insertions(+) diff --git a/src/main/java/org/broad/igv/oauth/OAuthUtils.java b/src/main/java/org/broad/igv/oauth/OAuthUtils.java index f7f73f6208..12a1f03e5f 100644 --- a/src/main/java/org/broad/igv/oauth/OAuthUtils.java +++ b/src/main/java/org/broad/igv/oauth/OAuthUtils.java @@ -143,6 +143,9 @@ public void updateOauthProvider(String provisioningURL) throws IOException { private String loadAsString(String urlOrPath) throws IOException { + if(HttpUtils.isRemoteURL(urlOrPath)) { + urlOrPath = HttpUtils.mapURL(urlOrPath); + } InputStream is = null; try { is = openInputStream(urlOrPath); From 73705563227dec764ff469d1364122148a3ef38b Mon Sep 17 00:00:00 2001 From: Louis Bergelson Date: Wed, 5 Apr 2023 18:37:13 -0400 Subject: [PATCH 041/116] Update htsjdk to 3.0.5 (#1290) * This includes a fix for https://github.com/igvteam/igv/issues/1286 --- build.gradle | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/build.gradle b/build.gradle index fdccc5047c..0adbbfc315 100644 --- a/build.gradle +++ b/build.gradle @@ -111,7 +111,7 @@ dependencies { [group: 'org.xerial.snappy', name: 'snappy-java', version: '1.1.7.3'], [group: 'org.apache.commons', name: 'commons-jexl', version: '2.1.1'], [group: 'org.apache.commons', name: 'commons-lang3', version: '3.9'], - [group: 'com.github.samtools', name: 'htsjdk', version: '3.0.0'], + [group: 'com.github.samtools', name: 'htsjdk', version: '3.0.5'], [group: 'org.swinglabs', name: 'swing-layout', version: '1.0.3'], //[group: 'org.bidib.com.jidesoft', name: 'jide-common', version: '3.7.3'], // no longer in Maven Central [group: 'com.google.guava', name: 'guava', version: '27.0.1-jre'], From 55d1923521badb0712d5482bf782256e997c2a2b Mon Sep 17 00:00:00 2001 From: Louis Bergelson Date: Wed, 5 Apr 2023 18:37:30 -0400 Subject: [PATCH 042/116] Add the option to split screen chimeric reads. (#1278) * Adding option to split screen to show supplementary (chimeric) reads * move comparatooor * refactoring some of the setGeneList stuff to make it less confusing --- .../java/org/broad/igv/lists/GeneList.java | 2 +- .../org/broad/igv/sam/AlignmentTrackMenu.java | 133 +++++++++--------- src/main/java/org/broad/igv/sam/ReadMate.java | 15 +- .../broad/igv/sam/SupplementaryAlignment.java | 27 +++- .../java/org/broad/igv/session/Session.java | 7 +- .../org/broad/igv/ui/panel/FrameManager.java | 56 ++++---- 6 files changed, 135 insertions(+), 105 deletions(-) diff --git a/src/main/java/org/broad/igv/lists/GeneList.java b/src/main/java/org/broad/igv/lists/GeneList.java index 02506a7a1b..b9b7a0ee9e 100644 --- a/src/main/java/org/broad/igv/lists/GeneList.java +++ b/src/main/java/org/broad/igv/lists/GeneList.java @@ -168,6 +168,6 @@ public void setDescription(String description) { // } public void sort(Comparator comparator) { - Collections.sort(this.loci, comparator); + this.loci.sort(comparator); } } diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index 645ce451e7..6fa4edfed1 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -1,10 +1,11 @@ package org.broad.igv.sam; +import htsjdk.samtools.SAMTag; +import htsjdk.samtools.util.Locatable; import org.broad.igv.Globals; import org.broad.igv.feature.Locus; import org.broad.igv.feature.Range; import org.broad.igv.feature.Strand; -import org.broad.igv.feature.genome.ChromosomeNameComparator; import org.broad.igv.jbrowse.CircularViewUtilities; import org.broad.igv.lists.GeneList; import org.broad.igv.logging.LogManager; @@ -12,7 +13,6 @@ import org.broad.igv.prefs.Constants; import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.sashimi.SashimiPlot; -import org.broad.igv.session.Session; import org.broad.igv.tools.PFMExporter; import org.broad.igv.track.SequenceTrack; import org.broad.igv.track.Track; @@ -39,6 +39,7 @@ import java.util.*; import java.util.List; import java.util.function.BiConsumer; +import java.util.stream.Collectors; import static org.broad.igv.prefs.Constants.*; @@ -125,7 +126,10 @@ class AlignmentTrackMenu extends IGVPopupMenu { if (clickedAlignment != null) { addGoToMate(e, clickedAlignment); showMateRegion(e, clickedAlignment); + //Supplementary/chimeric items, only if the read has an SA tag + addShowChimericRegions(alignmentTrack, e, clickedAlignment); } + addInsertSizeMenuItem(); // Third gen (primarily) items @@ -171,6 +175,27 @@ class AlignmentTrackMenu extends IGVPopupMenu { } + private void addShowChimericRegions(final AlignmentTrack alignmentTrack, final TrackClickEvent e, final Alignment clickedAlignment) { + JMenuItem item = new JMenuItem("View chimeric alignments in split screen"); + if (clickedAlignment.getAttribute(SAMTag.SA.name()) != null) { + item.setEnabled(true); + item.addActionListener(aEvt -> { + final String saTag = clickedAlignment.getAttribute(SAMTag.SA.name()).toString(); + try { + List supplementaryAlignments = SupplementaryAlignment.parseFromSATag(saTag); + alignmentTrack.setSelectedAlignment(clickedAlignment); + addNewLociToFrames(e.getFrame(), supplementaryAlignments); + } catch (final Exception ex) { + MessageUtils.showMessage("Failed to handle SA tag: " + saTag + " due to " + ex.getMessage()); + item.setEnabled(false); + } + }); + } else { + item.setEnabled(false); + } + add(item); + } + private void addHaplotype(TrackClickEvent e) { @@ -1155,79 +1180,57 @@ private void gotoMate(final ReferenceFrame frame, Alignment alignment) { * Need a better name for this method. */ private void splitScreenMate(ReferenceFrame frame, Alignment alignment) { - if (alignment != null) { ReadMate mate = alignment.getMate(); if (mate != null && mate.isMapped()) { - alignmentTrack.setSelectedAlignment(alignment); - - String mateChr = mate.getChr(); - int mateStart = mate.start - 1; - - String locus1 = frame.getFormattedLocusString(); - - // Generate a locus string for the read mate. Keep the window width (in base pairs) == to the current range - Range range = frame.getCurrentRange(); - int length = range.getLength(); - int s2 = Math.max(0, mateStart - length / 2); - int e2 = s2 + length; - String startStr = String.valueOf(s2); - String endStr = String.valueOf(e2); - String mateLocus = mateChr + ":" + startStr + "-" + endStr; - - Session currentSession = IGV.getInstance().getSession(); - - List loci; - if (FrameManager.isGeneListMode()) { - loci = new ArrayList<>(FrameManager.getFrames().size()); - for (ReferenceFrame ref : FrameManager.getFrames()) { - //If the frame-name is a locus, we use it unaltered - //Don't want to reprocess, easy to get off-by-one - String name = ref.getName(); - if (Locus.fromString(name) != null) { - loci.add(name); - } else { - loci.add(ref.getFormattedLocusString()); - } - - } - loci.add(mateLocus); - } else { - loci = Arrays.asList(locus1, mateLocus); - } - - StringBuilder listName = new StringBuilder(); - for (String s : loci) { - listName.append(s + " "); - } - - GeneList geneList = new GeneList(listName.toString(), loci, false); - currentSession.setCurrentGeneList(geneList); - - Comparator geneListComparator = (n0, n1) -> { - ReferenceFrame f0 = FrameManager.getFrame(n0); - ReferenceFrame f1 = FrameManager.getFrame(n1); - - String chr0 = f0 == null ? "" : f0.getChrName(); - String chr1 = f1 == null ? "" : f1.getChrName(); - int s0 = f0 == null ? 0 : f0.getCurrentRange().getStart(); - int s1 = f1 == null ? 0 : f1.getCurrentRange().getStart(); - - int chrComp = ChromosomeNameComparator.get().compare(chr0, chr1); - if (chrComp != 0) return chrComp; - return s0 - s1; - }; - - //Need to sort the frames by position - currentSession.sortGeneList(geneListComparator); - IGV.getInstance().resetFrames(); + addNewLociToFrames(frame, List.of(mate)); } else { MessageUtils.showMessage("Alignment does not have mate, or it is not mapped."); } } } + private static void addNewLociToFrames(final ReferenceFrame frame, final List toIncludeInSplit) { + final List newLoci = toIncludeInSplit.stream() + .map(locatable -> getLocusStringForAlignment(frame, locatable)) + .collect(Collectors.toList()); + List loci = createLociList(frame, newLoci); + String listName = String.join(" ", loci); // TODO check the trailing " " was unnecessary + //Need to sort the frames by position + GeneList geneList = new GeneList(listName, loci, false); + geneList.sort(FrameManager.FRAME_COMPARATOR); + IGV.getInstance().getSession().setCurrentGeneList(geneList); + IGV.getInstance().resetFrames(); + } + + private static List createLociList(final ReferenceFrame frame, final List lociToAdd) { + final List loci = new ArrayList<>(FrameManager.getFrames().size() + lociToAdd.size()); + if (FrameManager.isGeneListMode()) { + for (ReferenceFrame ref : FrameManager.getFrames()) { + //If the frame-name is a locus, we use it unaltered + //Don't want to reprocess, easy to get off-by-one + String name = ref.getName(); + loci.add(Locus.fromString(name) != null ? name : ref.getFormattedLocusString()); + } + } else { + loci.add(frame.getFormattedLocusString()); + } + loci.addAll(lociToAdd); + return loci; + } + + private static String getLocusStringForAlignment(final ReferenceFrame frame, final Locatable alignment) { + int adjustedMateStart = alignment.getStart() - 1; + + // Generate a locus string for the alignment. Keep the window width (in base pairs) == to the current range + Range range = frame.getCurrentRange(); + int length = range.getLength(); + int start = Math.max(0, adjustedMateStart - length / 2); + int end = start + length; + return alignment.getContig() + ":" + start + "-" + end; + } + /** * Get the most "specific" alignment at the specified location. Specificity refers to the smallest alignment diff --git a/src/main/java/org/broad/igv/sam/ReadMate.java b/src/main/java/org/broad/igv/sam/ReadMate.java index 605f742dbd..7c34767214 100644 --- a/src/main/java/org/broad/igv/sam/ReadMate.java +++ b/src/main/java/org/broad/igv/sam/ReadMate.java @@ -30,9 +30,10 @@ package org.broad.igv.sam; +import htsjdk.samtools.util.Locatable; import org.broad.igv.feature.Strand; -public class ReadMate { +public class ReadMate implements Locatable { private String chr; int start; @@ -55,10 +56,20 @@ public String positionString() { return chr + ":" + start + " (" + (isNegativeStrand() ? "-" : "+") + ")"; } + @Override + public String getContig() { + return chr; + } + public int getStart() { return start; } + @Override + public int getEnd() { + throw new UnsupportedOperationException("ReadMate has an unknown end."); + } + public boolean isNegativeStrand() { return negativeStrand; } @@ -68,6 +79,6 @@ public Strand getStrand() { } public String getChr() { - return chr; + return getContig(); } } diff --git a/src/main/java/org/broad/igv/sam/SupplementaryAlignment.java b/src/main/java/org/broad/igv/sam/SupplementaryAlignment.java index 8912873233..bb5cf87a67 100644 --- a/src/main/java/org/broad/igv/sam/SupplementaryAlignment.java +++ b/src/main/java/org/broad/igv/sam/SupplementaryAlignment.java @@ -1,8 +1,13 @@ package org.broad.igv.sam; +import htsjdk.samtools.util.Locatable; import org.broad.igv.Globals; -public class SupplementaryAlignment { +import java.util.Arrays; +import java.util.List; +import java.util.stream.Collectors; + +public class SupplementaryAlignment implements Locatable { public String chr; public int start; @@ -28,6 +33,11 @@ public String printString() { + " (" + strand + ") = " + Globals.DECIMAL_FORMAT.format(lenOnRef) + "bp @MAPQ " + mapQ + " NM" + numMismatches; } + public static List parseFromSATag(String saTag){ + return Arrays.stream(Globals.semicolonPattern.split(saTag)) + .map(SupplementaryAlignment::new) + .collect(Collectors.toList()); + } int computeLengthOnReference(String cigarString) { @@ -53,4 +63,19 @@ int computeLengthOnReference(String cigarString) { } return len; } + + @Override + public String getContig() { + return chr; + } + + @Override + public int getStart() { + return start; + } + + @Override + public int getEnd() { + return start + lenOnRef; + } } diff --git a/src/main/java/org/broad/igv/session/Session.java b/src/main/java/org/broad/igv/session/Session.java index 957a442c52..9c6d6dfce3 100644 --- a/src/main/java/org/broad/igv/session/Session.java +++ b/src/main/java/org/broad/igv/session/Session.java @@ -415,11 +415,6 @@ public void setCurrentGeneList(GeneList currentGeneList) { } } - public void sortGeneList(Comparator comparator) { - getCurrentGeneList().sort(comparator); - this.setCurrentGeneList(getCurrentGeneList()); - } - public int getVersion() { return version; } @@ -486,7 +481,7 @@ static class Locus { * position string. UCSC conventions are followed for coordinates, * specifically the internal representation is "zero" based (first base is * numbered 0) but the display representation is "one" based (first base is - * numbered 1). Consequently 1 is substracted from the parsed positions + * numbered 1). Consequently, 1 is subtracted from the parsed positions */ private static int[] getStartEnd(String posString) { try { diff --git a/src/main/java/org/broad/igv/ui/panel/FrameManager.java b/src/main/java/org/broad/igv/ui/panel/FrameManager.java index 042a0c00ce..85c46c0d53 100644 --- a/src/main/java/org/broad/igv/ui/panel/FrameManager.java +++ b/src/main/java/org/broad/igv/ui/panel/FrameManager.java @@ -36,7 +36,6 @@ import org.broad.igv.lists.GeneList; import org.broad.igv.prefs.Constants; import org.broad.igv.prefs.PreferencesManager; -import org.broad.igv.session.Session; import org.broad.igv.track.RegionScoreType; import org.broad.igv.track.Track; import org.broad.igv.ui.IGV; @@ -52,6 +51,20 @@ */ public class FrameManager implements IGVEventObserver { + public static final Comparator FRAME_COMPARATOR = (n0, n1) -> { + ReferenceFrame f0 = getFrame(n0); + ReferenceFrame f1 = getFrame(n1); + + String chr0 = f0 == null ? "" : f0.getChrName(); + String chr1 = f1 == null ? "" : f1.getChrName(); + int s0 = f0 == null ? 0 : f0.getCurrentRange().getStart(); + int s1 = f1 == null ? 0 : f1.getCurrentRange().getStart(); + + int chrComp = ChromosomeNameComparator.get().compare(chr0, chr1); + if (chrComp != 0) return chrComp; + return s0 - s1; + }; + private static List frames = new ArrayList(); private static ReferenceFrame defaultFrame; @@ -139,7 +152,7 @@ public static void resetFrames(GeneList gl) { if (gl == null) { frames.add(getDefaultFrame()); } else { - List lociNotFound = new ArrayList(); + List lociNotFound = new ArrayList<>(); List loci = gl.getLoci(); if (loci.size() == 1) { Locus locus = getLocus(loci.get(0)); @@ -247,17 +260,14 @@ public static void removeFrame(ReferenceFrame frame) { public static void sortFrames(final Track t) { - Collections.sort(frames, new Comparator() { - @Override - public int compare(ReferenceFrame o1, ReferenceFrame o2) { - float s1 = t.getRegionScore(o1.getChromosome().getName(), (int) o1.getOrigin(), (int) o1.getEnd(), - o1.getZoom(), RegionScoreType.SCORE, o1.getName()); - float s2 = t.getRegionScore(o2.getChromosome().getName(), (int) o2.getOrigin(), (int) o2.getEnd(), - o2.getZoom(), RegionScoreType.SCORE, o2.getName()); - return (s1 == s2 ? 0 : (s1 > s2) ? -1 : 1); - } - }); - + frames.sort(Comparator.comparingDouble((ReferenceFrame f) -> + t.getRegionScore(f.getChromosome().getName(), + (int) f.getOrigin(), + (int) f.getEnd(), + f.getZoom(), + RegionScoreType.SCORE, + f.getName())) + .reversed()); } /** @@ -338,24 +348,10 @@ public static void addFrames(List newLociStrings) { } } + //Need to sort the frames by position GeneList geneList = new GeneList("Current frames", loci, false); - Session currentSession = IGV.getInstance().getSession(); - currentSession.setCurrentGeneList(geneList); - - // sort the frames by position - currentSession.sortGeneList((n0, n1) -> { - ReferenceFrame f0 = getFrame(n0); - ReferenceFrame f1 = getFrame(n1); - - String chr0 = f0 == null ? "" : f0.getChrName(); - String chr1 = f1 == null ? "" : f1.getChrName(); - int s0 = f0 == null ? 0 : f0.getCurrentRange().getStart(); - int s1 = f1 == null ? 0 : f1.getCurrentRange().getStart(); - - int chrComp = ChromosomeNameComparator.get().compare(chr0, chr1); - if (chrComp != 0) return chrComp; - return s0 - s1; - }); + geneList.sort(FRAME_COMPARATOR); + IGV.getInstance().getSession().setCurrentGeneList(geneList); IGV.getInstance().resetFrames(); } From 7d893e11c3376fbf118bc15a9f62660a8979720f Mon Sep 17 00:00:00 2001 From: Kevin Lydon Date: Wed, 5 Apr 2023 18:39:31 -0400 Subject: [PATCH 043/116] Autosaves session to a file in the igv folder when exiting. Added option in preferences to enable automatically loading autosave when starting IGV. (#1309) Modified quit handler to not call shutdown twice, which seemed to cause the saving to happen twice. --- .../java/org/broad/igv/DirectoryManager.java | 26 +++++++++++++++++++ .../java/org/broad/igv/prefs/Constants.java | 1 + .../org/broad/igv/ui/DesktopIntegration.java | 8 ++---- src/main/java/org/broad/igv/ui/IGV.java | 16 ++++++++++++ .../org/broad/igv/prefs/defaults_2.3.tab | 1 + .../org/broad/igv/prefs/preferences.tab | 1 + 6 files changed, 47 insertions(+), 6 deletions(-) diff --git a/src/main/java/org/broad/igv/DirectoryManager.java b/src/main/java/org/broad/igv/DirectoryManager.java index 605d4ec05d..316efff4f0 100644 --- a/src/main/java/org/broad/igv/DirectoryManager.java +++ b/src/main/java/org/broad/igv/DirectoryManager.java @@ -263,6 +263,32 @@ public static synchronized File getLogFile() throws IOException { } + /** + * Creates and returns a file object for the file where a user's session is autosaved on exit. + * + * @return File object for the session autosave file + */ + public static File getAutosavedSession() { + File autosavedSessionFile = new File(getIgvDirectory(), "session_autosave.xml"); + return autosavedSessionFile; + } + + /** + * Creates and returns a file object for the file where a user's session is autosaved on exit. + * If the file does not exist in the file system (at "~/igv/session_autosave.xml"), creates it. + * + * @return File object for the session autosave file + * @throws IOException + */ + public static synchronized File getAutosavedSessionCreateIfNotExists() throws IOException { + + File autosavedSessionFile = new File(getIgvDirectory(), "session_autosave.xml"); + if (!autosavedSessionFile.exists()) { + autosavedSessionFile.createNewFile(); + } + return autosavedSessionFile; + } + /** * Return the user preferences property file ("~/igv/prefs.properties"). diff --git a/src/main/java/org/broad/igv/prefs/Constants.java b/src/main/java/org/broad/igv/prefs/Constants.java index 787223c720..c38c29818c 100644 --- a/src/main/java/org/broad/igv/prefs/Constants.java +++ b/src/main/java/org/broad/igv/prefs/Constants.java @@ -34,6 +34,7 @@ private Constants() { public static final String SHOW_GENOME_SERVER_WARNING = "SHOW_GENOME_SERVER_WARNING"; public static final String CN_FREQ_AMP_THRESHOLD = "CN_FREQ.AMP_THRESHOLD"; public static final String CN_FREQ_DEL_THRESHOLD = "CN_FREQ.DEL_THRESHOLD"; + public static final String AUTOLOAD_LAST_AUTOSAVE = "AUTOLOAD_LAST_AUTOSAVE"; // public static final String RECENT_SESSIONS = "IGV.Session.recent.sessions"; diff --git a/src/main/java/org/broad/igv/ui/DesktopIntegration.java b/src/main/java/org/broad/igv/ui/DesktopIntegration.java index a20751c58f..fd995fa692 100644 --- a/src/main/java/org/broad/igv/ui/DesktopIntegration.java +++ b/src/main/java/org/broad/igv/ui/DesktopIntegration.java @@ -58,14 +58,10 @@ public static void setDockIcon(Image image) { public static void setAboutHandler(IGVMenuBar igvMenuBar) { Desktop.getDesktop().setAboutHandler(e -> igvMenuBar.showAboutDialog()); } - + public static void setQuitHandler() { Desktop.getDesktop().setQuitHandler((e, response) -> { - try { - ShutdownThread.runS(); - } finally { - response.performQuit(); - } + response.performQuit(); }); } } diff --git a/src/main/java/org/broad/igv/ui/IGV.java b/src/main/java/org/broad/igv/ui/IGV.java index 06f9e7e10b..b304116075 100644 --- a/src/main/java/org/broad/igv/ui/IGV.java +++ b/src/main/java/org/broad/igv/ui/IGV.java @@ -248,6 +248,12 @@ public void windowGainedFocus(WindowEvent windowEvent) { } mainFrame.setBounds(applicationBounds); + // Autoload session autosave if user preferences say to do that + if (DirectoryManager.getAutosavedSession().exists() && PreferencesManager.getPreferences().getAsBoolean(AUTOLOAD_LAST_AUTOSAVE)) { + File sessionAutosave = DirectoryManager.getAutosavedSession(); + loadSession(sessionAutosave.getAbsolutePath(), null); + } + subscribeToEvents(); } @@ -561,6 +567,16 @@ final public void saveStateForExit() { PreferencesManager.getPreferences().remove(RECENT_SESSIONS); PreferencesManager.getPreferences().setRecentSessions(recentSessions); } + + // Autosave current session + try { + File sessionAutosave = DirectoryManager.getAutosavedSessionCreateIfNotExists(); + saveSession(sessionAutosave); + } + catch(Exception e) { + log.error("Error autosaving session", e); + } + } final public void doShowAttributeDisplay(boolean enableAttributeView) { diff --git a/src/main/resources/org/broad/igv/prefs/defaults_2.3.tab b/src/main/resources/org/broad/igv/prefs/defaults_2.3.tab index ec9c16e2c4..004a16a36b 100644 --- a/src/main/resources/org/broad/igv/prefs/defaults_2.3.tab +++ b/src/main/resources/org/broad/igv/prefs/defaults_2.3.tab @@ -1,6 +1,7 @@ AF_REF.COLOR 0,0,220 AF_VAR.COLOR 255,0,0 AUTO_UPDATE_GENOMES true +AUTOLOAD_LAST_AUTOSAVE false BACKGROUND_COLOR 250,250,250 BLAT_URL http://genome.cse.ucsc.edu/cgi-bin/hgBlat BYPASS_FILE_AUTO_DISCOVERY false diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index d6b46672a2..a61daf19bc 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -1,5 +1,6 @@ #General SESSION.RELATIVE_PATH Use relative paths in session files boolean TRUE +AUTOLOAD_LAST_AUTOSAVE Load last autosaved session on startup boolean FALSE IGV.single.track.pane Display all tracks in a single panel boolean FALSE IGV.track.show.attribute.views Show attribute panel boolean FALSE SHOW_DEFAULT_TRACK_ATTRIBUTES Show default track attributes boolean FALSE From 4e5df4c41ec74362e8f24e38247d1bf7239b72d4 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 5 Apr 2023 21:31:42 -0700 Subject: [PATCH 044/116] Use POST for UCSC blat calls --- .../org/broad/igv/util/blat/BlatClient.java | 19 +++++++--- .../org/broad/igv/prefs/preferences.tab | 2 +- .../broad/igv/util/blat/BlatClientTest.java | 36 ++++++++++++++----- 3 files changed, 44 insertions(+), 13 deletions(-) diff --git a/src/main/java/org/broad/igv/util/blat/BlatClient.java b/src/main/java/org/broad/igv/util/blat/BlatClient.java index 6fac19bd40..f94cfcb126 100644 --- a/src/main/java/org/broad/igv/util/blat/BlatClient.java +++ b/src/main/java/org/broad/igv/util/blat/BlatClient.java @@ -68,13 +68,12 @@ public static List blat(String db, String userSeq) throws IOException } urlpref = urlpref.trim(); - if (serverType.equalsIgnoreCase("web_blat") || !urlpref.contains("$SEQUENCE")) { + if (serverType.equalsIgnoreCase("web_blat")) { return LegacyBlatClient.blat(userSeq); } else { String dbEncoded = URLEncoder.encode(db, "UTF-8"); - urlpref = urlpref.replace("$SEQUENCE", userSeq).replace("$DB", dbEncoded); //Strip leading "file://" protocol, if any if (urlpref.startsWith("file://")) { @@ -84,12 +83,24 @@ public static List blat(String db, String userSeq) throws IOException String jsonString = null; try { - // If urlpref is not a URL, assume it is a command line program. An example might be // blat.sh $SEQUENCE $DB if (URLUtils.isURL(urlpref)) { - jsonString = HttpUtils.getInstance().getContentsAsJSON(new URL(urlpref)); + if (urlpref.contains("$SEQUENCE")) { + // Old "GET" style url + urlpref = urlpref.replace("$SEQUENCE", userSeq).replace("$DB", dbEncoded); + jsonString = HttpUtils.getInstance().getContentsAsJSON(new URL(urlpref)); + } else { + // New "POST" style url -- can handle large sequences + Map params = new HashMap(); + params.put("userSeq", userSeq); + params.put("db", dbEncoded); + params.put("type", "DNA"); + params.put("output", "json"); + jsonString = HttpUtils.getInstance().doPost(new URL(urlpref), params); + } + } else { jsonString = RuntimeUtils.exec(urlpref); } diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index a61daf19bc..88b9cc1613 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -219,7 +219,7 @@ MASTER_RESOURCE_FILE_KEY Data registry URL string https://data.broadinstitute.or PROVISIONING.URL OAuth provisioning URL string null --- -BLAT_URL BLAT URL String http://genome.ucsc.edu/cgi-bin/hgBlat?userSeq=$SEQUENCE&type=DNA&db=$DB&output=json +BLAT_URL BLAT URL String http://genome.ucsc.edu/cgi-bin/hgBlat --- ## Tooltip diff --git a/src/test/java/org/broad/igv/util/blat/BlatClientTest.java b/src/test/java/org/broad/igv/util/blat/BlatClientTest.java index be910d2179..0b2076651e 100644 --- a/src/test/java/org/broad/igv/util/blat/BlatClientTest.java +++ b/src/test/java/org/broad/igv/util/blat/BlatClientTest.java @@ -1,7 +1,10 @@ package org.broad.igv.util.blat; +import org.broad.igv.AbstractHeadlessTest; import org.broad.igv.feature.PSLRecord; import org.broad.igv.feature.Strand; +import org.broad.igv.prefs.Constants; +import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.ui.util.MessageUtils; import org.junit.Test; @@ -10,18 +13,36 @@ import static org.junit.Assert.*; -public class BlatClientTest { +public class BlatClientTest extends AbstractHeadlessTest { @Test - public void blat() throws IOException { + public void blatGET() throws IOException { //https://genome.ucsc.edu/cgi-bin/hgBlat?userSeq=GTCCTCGGAACCAGGACCTCGGCGTGGCCTAGCG&type=DNA&db=hg19&output=json - List results = BlatClient.blat("hg19", "GTCCTCGGAACCAGGACCTCGGCGTGGCCTAGCG"); + //http://genome.ucsc.edu/cgi-bin/hgBlat?userSeq=$SEQUENCE&type=DNA&db=$DB&output=json - assertEquals(1, results.size()); - assertEquals("chr21", results.get(0).getChr()); - assertEquals(Strand.POSITIVE, results.get(0).getStrand()); - assertEquals(34, results.get(0).getqSize()); + PreferencesManager.getPreferences().put(Constants.BLAT_URL, "https://genome.ucsc.edu/cgi-bin/hgBlat?userSeq=$SEQUENCE&type=DNA&db=$DB&output=json"); + List results = BlatClient.blat("hg19", "GTCCTCGGAACCAGGACCTCGGCGTGGCCTAGCG"); + + assertEquals(1, results.size()); + assertEquals("chr21", results.get(0).getChr()); + assertEquals(Strand.POSITIVE, results.get(0).getStrand()); + assertEquals(34, results.get(0).getqSize()); + } + + @Test + public void blatPOST() throws IOException { + + //https://genome.ucsc.edu/cgi-bin/hgBlat?userSeq=GTCCTCGGAACCAGGACCTCGGCGTGGCCTAGCG&type=DNA&db=hg19&output=json + //http://genome.ucsc.edu/cgi-bin/hgBlat?userSeq=$SEQUENCE&type=DNA&db=$DB&output=json + + PreferencesManager.getPreferences().put(Constants.BLAT_URL, "https://genome.ucsc.edu/cgi-bin/hgBlat"); + List results = BlatClient.blat("hg19", "GTCCTCGGAACCAGGACCTCGGCGTGGCCTAGCG"); + + assertEquals(1, results.size()); + assertEquals("chr21", results.get(0).getChr()); + assertEquals(Strand.POSITIVE, results.get(0).getStrand()); + assertEquals(34, results.get(0).getqSize()); } @Test @@ -39,7 +60,6 @@ public void blatTooShort() throws IOException { } - // public static void main(String [] args) throws IOException { // (new BlatClientTest()).blatTooShort(); // } From fe691f03b92c0b187f451e367db269d88dbab880 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Tue, 18 Apr 2023 13:27:40 -0700 Subject: [PATCH 045/116] fix -- show all bases broken --- .../java/org/broad/igv/sam/AlignmentRenderer.java | 12 ++++++------ 1 file changed, 6 insertions(+), 6 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java index e9743bdacc..aba8df86cc 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java +++ b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java @@ -801,7 +801,7 @@ private void drawAlignment( if (bisulfiteMode) { color = bisinfo.getDisplayColor(idx); } else if (colorOption.isBaseMod() || - colorOption.isSMRTKinetics()) { + colorOption.isSMRTKinetics()) { color = Color.GRAY; } else { color = nucleotideColors.get(c); @@ -817,8 +817,8 @@ private void drawAlignment( BisulfiteBaseInfo.DisplayStatus bisstatus = (bisinfo == null) ? null : bisinfo.getDisplayStatus(idx); - final boolean showBase = - isSoftClip || + final boolean showBase = showAllBases || + isSoftClip || bisulfiteMode || // In "quick consensus" mode, only show mismatches at positions with a consistent alternative basepair. (!quickConsensus || alignmentCounts.isConsensusMismatch(loc, reference[idx], chr, snpThreshold)); @@ -1265,10 +1265,10 @@ private Color getAlignmentColor(Alignment alignment, AlignmentTrack track) { } break; case READ_ORDER: - if (alignment.isPaired()){ - if(alignment.isFirstOfPair() && !alignment.isSecondOfPair()){ + if (alignment.isPaired()) { + if (alignment.isFirstOfPair() && !alignment.isSecondOfPair()) { c = firstOfPairColor; - } else if(!alignment.isFirstOfPair() && alignment.isSecondOfPair()) { + } else if (!alignment.isFirstOfPair() && alignment.isSecondOfPair()) { c = secondOfPairColor; } else if (alignment.isFirstOfPair() && alignment.isSecondOfPair()) { c = firstAndSecondofPairColor; From bc0292017bd82a914714a7dfa37be5a09dfd1e5c Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Tue, 18 Apr 2023 13:28:16 -0700 Subject: [PATCH 046/116] move "View chimeric alignments" menu item to 3rd gene groupings --- .../org/broad/igv/sam/AlignmentTrackMenu.java | 15 +++++++-------- 1 file changed, 7 insertions(+), 8 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index 6fa4edfed1..774b4e3f3a 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -10,7 +10,6 @@ import org.broad.igv.lists.GeneList; import org.broad.igv.logging.LogManager; import org.broad.igv.logging.Logger; -import org.broad.igv.prefs.Constants; import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.sashimi.SashimiPlot; import org.broad.igv.tools.PFMExporter; @@ -126,15 +125,12 @@ class AlignmentTrackMenu extends IGVPopupMenu { if (clickedAlignment != null) { addGoToMate(e, clickedAlignment); showMateRegion(e, clickedAlignment); - //Supplementary/chimeric items, only if the read has an SA tag - addShowChimericRegions(alignmentTrack, e, clickedAlignment); } - addInsertSizeMenuItem(); // Third gen (primarily) items addSeparator(); - addThirdGenItems(); + addThirdGenItems(clickedAlignment, e); // Display mode items addSeparator(); @@ -171,11 +167,10 @@ class AlignmentTrackMenu extends IGVPopupMenu { // Show alignments, coverage, splice junctions addSeparator(); addShowItems(); - - } private void addShowChimericRegions(final AlignmentTrack alignmentTrack, final TrackClickEvent e, final Alignment clickedAlignment) { + JMenuItem item = new JMenuItem("View chimeric alignments in split screen"); if (clickedAlignment.getAttribute(SAMTag.SA.name()) != null) { item.setEnabled(true); @@ -1082,7 +1077,11 @@ private void addInsertionItems(AlignmentBlock insertion) { } } - void addThirdGenItems() { + void addThirdGenItems(Alignment clickedAlignment, final TrackClickEvent tce) { + + //Supplementary/chimeric items, only if the read has an SA tag + addShowChimericRegions(alignmentTrack, tce, clickedAlignment); + final JMenuItem qcItem = new JCheckBoxMenuItem("Quick consensus mode"); qcItem.setSelected(renderOptions.isQuickConsensusMode()); From 798d043dd4af7b854a888ba27af257f950d240ae Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 19 Apr 2023 00:00:39 -0400 Subject: [PATCH 047/116] Various NPE exceptions in VariantTrack on "allSamples" member -- this can be null (apparently) --- .../org/broad/igv/variant/VariantMenu.java | 2 +- .../org/broad/igv/variant/VariantTrack.java | 42 +++++++++++-------- 2 files changed, 26 insertions(+), 18 deletions(-) diff --git a/src/main/java/org/broad/igv/variant/VariantMenu.java b/src/main/java/org/broad/igv/variant/VariantMenu.java index 56b781921b..00808d28da 100644 --- a/src/main/java/org/broad/igv/variant/VariantMenu.java +++ b/src/main/java/org/broad/igv/variant/VariantMenu.java @@ -112,7 +112,7 @@ public VariantMenu(final VariantTrack variantTrack, final Variant variant, Track } // Show genotypes - if (track.getAllSamples().size() > 0) { + if (track.sampleCount() > 0) { addSeparator(); add(getShowGenotypes()); } diff --git a/src/main/java/org/broad/igv/variant/VariantTrack.java b/src/main/java/org/broad/igv/variant/VariantTrack.java index 8d7bacc484..b3e12f643b 100644 --- a/src/main/java/org/broad/igv/variant/VariantTrack.java +++ b/src/main/java/org/broad/igv/variant/VariantTrack.java @@ -223,7 +223,7 @@ public VariantTrack(ResourceLocator locator, FeatureSource source, List setDisplayMode(DisplayMode.EXPANDED); - int sampleCount = allSamples.size(); + int sampleCount = sampleCount(); final int groupCount = samplesByGroups.size(); final int margins = (groupCount - 1) * 3; squishedHeight = sampleCount == 0 || showGenotypes == false ? DEFAULT_SQUISHED_HEIGHT : @@ -262,7 +262,7 @@ public VariantTrack(ResourceLocator locator, FeatureSource source, List if (defVisibilityWindow > 0) { setVisibilityWindow(defVisibilityWindow * 1000); } else { - int vw = Math.max(10000, (100000 - 100 * (allSamples.size() - 1))); + int vw = Math.max(10000, (100000 - 100 * (sampleCount() - 1))); setVisibilityWindow(vw); } } @@ -272,7 +272,7 @@ public VariantTrack(ResourceLocator locator, FeatureSource source, List } private boolean defaultShowGenotypes() { - return allSamples.size() > 0; + return sampleCount() > 0; } private void loadAlignmentMappings(String bamListPath) { @@ -349,16 +349,18 @@ private void setupGroupsFromAttributes() { return; } - for (String sample : allSamples) { + if(allSamples != null) { + for (String sample : allSamples) { - String sampleGroup = manager.getAttribute(sample, newGroupByAttribute); + String sampleGroup = manager.getAttribute(sample, newGroupByAttribute); - List sampleList = samplesByGroups.get(sampleGroup); - if (sampleList == null) { - sampleList = new ArrayList(); - samplesByGroups.put(sampleGroup, sampleList); + List sampleList = samplesByGroups.get(sampleGroup); + if (sampleList == null) { + sampleList = new ArrayList(); + samplesByGroups.put(sampleGroup, sampleList); + } + sampleList.add(sample); } - sampleList.add(sample); } grouped = samplesByGroups.size() > 1; @@ -371,9 +373,11 @@ private void setupGroupsFromAttributes() { * @param comparator the comparator to sort by */ public void sortSamples(Comparator comparator) { - Collections.sort(allSamples, comparator); - for (List samples : samplesByGroups.values()) { - Collections.sort(samples, comparator); + if(allSamples != null) { + Collections.sort(allSamples, comparator); + for (List samples : samplesByGroups.values()) { + Collections.sort(samples, comparator); + } } } @@ -406,7 +410,7 @@ public int getGenotypeBandHeight() { * @return */ public int getHeight() { - int sampleCount = allSamples.size(); + int sampleCount = sampleCount(); int h; if (getDisplayMode() == DisplayMode.COLLAPSED || sampleCount == 0 || showGenotypes == false) { h = getVariantsHeight(); @@ -425,6 +429,10 @@ public Object getHeader() { return null; } + public int sampleCount() { + return allSamples == null ? 0 : allSamples.size(); + } + /** * Return the height of the variant section only (no samples/genotypes) * @@ -451,7 +459,7 @@ public void setHeight(int height) { // If height is < expanded height try "squishing" track, otherwise expand it final int groupCount = samplesByGroups.size(); final int margins = (groupCount - 1) * 3; - int sampleCount = showGenotypes == false ? 0 : allSamples.size(); + int sampleCount = showGenotypes == false ? 0 : sampleCount(); final int expandedHeight = getVariantBandHeight() + margins + (sampleCount * getGenotypeBandHeight()); if (height < expandedHeight) { setDisplayMode(DisplayMode.SQUISHED); @@ -584,7 +592,7 @@ private void drawLineIfVisible(Graphics2D g2D, Rectangle visibleRectangle, Color } private void drawVariantBandBorder(Graphics2D g2D, Rectangle visibleRectangle, int variantBandY, int left, int right) { - if (allSamples.size() > 0 && showGenotypes) { + if (sampleCount() > 0 && showGenotypes) { drawLineIfVisible(g2D, visibleRectangle, Color.lightGray, variantBandY, left, right); } } @@ -635,7 +643,7 @@ private void renderBoundaryLines(Graphics2D g2D, Rectangle trackRectangle, Recta drawLineIfVisible(g2D, visibleRectangle, borderGray, bottomY, left, right); // Variant / Genotype border - if (allSamples.size() > 0 && showGenotypes) { + if (sampleCount() > 0 && showGenotypes) { int variantGenotypeBorderY = trackRectangle.y + getVariantsHeight(); drawVariantBandBorder(g2D, visibleRectangle, variantGenotypeBorderY, left, right); From 2e803459951429c6d9ae6769cf62f790b9321217 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 19 Apr 2023 00:00:52 -0400 Subject: [PATCH 048/116] NPE fix --- src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index 774b4e3f3a..1dedbbac82 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -172,7 +172,7 @@ class AlignmentTrackMenu extends IGVPopupMenu { private void addShowChimericRegions(final AlignmentTrack alignmentTrack, final TrackClickEvent e, final Alignment clickedAlignment) { JMenuItem item = new JMenuItem("View chimeric alignments in split screen"); - if (clickedAlignment.getAttribute(SAMTag.SA.name()) != null) { + if (clickedAlignment != null && clickedAlignment.getAttribute(SAMTag.SA.name()) != null) { item.setEnabled(true); item.addActionListener(aEvt -> { final String saTag = clickedAlignment.getAttribute(SAMTag.SA.name()).toString(); From cef2198974f0af77ce3818ec983768b7aa1c3eb8 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 19 Apr 2023 00:54:51 -0400 Subject: [PATCH 049/116] Fix schatz sessions --- test/sessions/circview/schatz_paired-end.xml | 18 +++++++++--------- .../circview/schatz_skbr3_longreads.xml | 14 +++++++------- 2 files changed, 16 insertions(+), 16 deletions(-) diff --git a/test/sessions/circview/schatz_paired-end.xml b/test/sessions/circview/schatz_paired-end.xml index bc584b4410..f1387388d3 100644 --- a/test/sessions/circview/schatz_paired-end.xml +++ b/test/sessions/circview/schatz_paired-end.xml @@ -1,26 +1,26 @@ - + - + - + - + - + - + - + - + - + diff --git a/test/sessions/circview/schatz_skbr3_longreads.xml b/test/sessions/circview/schatz_skbr3_longreads.xml index eb8d9a69a3..9aceaeb348 100644 --- a/test/sessions/circview/schatz_skbr3_longreads.xml +++ b/test/sessions/circview/schatz_skbr3_longreads.xml @@ -1,26 +1,26 @@ - + - + - + - + - + - + - + From 6d39e5c64b2d899ff85aff3a5bcddccf68ebfcad Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Wed, 26 Apr 2023 13:54:42 -0700 Subject: [PATCH 050/116] Update license.txt --- license.txt | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/license.txt b/license.txt index 71939b5b51..c8a4dc5858 100644 --- a/license.txt +++ b/license.txt @@ -1,7 +1,7 @@ The MIT License (MIT) -Copyright (c) 2007-2022 Broad Institute and Regents of the University of California +Copyright (c) 2007-2023 Broad Institute and Regents of the University of California Permission is hereby granted, free of charge, to any person obtaining a copy From 9509213130ce1624e832a001d2d1f5aa361d9acb Mon Sep 17 00:00:00 2001 From: Kevin Lydon Date: Thu, 4 May 2023 23:29:11 -0400 Subject: [PATCH 051/116] Moved autosave logic to startup code so it won't be overwritten by loading default genomes. Also modified startup logic to only load default genome if not loading a session file and not autoload session if user has specified a session at the command line (#1317) --- src/main/java/org/broad/igv/ui/IGV.java | 42 ++++++++++++++----------- 1 file changed, 23 insertions(+), 19 deletions(-) diff --git a/src/main/java/org/broad/igv/ui/IGV.java b/src/main/java/org/broad/igv/ui/IGV.java index b304116075..1acf776cd5 100644 --- a/src/main/java/org/broad/igv/ui/IGV.java +++ b/src/main/java/org/broad/igv/ui/IGV.java @@ -248,12 +248,6 @@ public void windowGainedFocus(WindowEvent windowEvent) { } mainFrame.setBounds(applicationBounds); - // Autoload session autosave if user preferences say to do that - if (DirectoryManager.getAutosavedSession().exists() && PreferencesManager.getPreferences().getAsBoolean(AUTOLOAD_LAST_AUTOSAVE)) { - File sessionAutosave = DirectoryManager.getAutosavedSession(); - loadSession(sessionAutosave.getAbsolutePath(), null); - } - subscribeToEvents(); } @@ -1936,8 +1930,10 @@ public void run() { } } else { - boolean genomeLoaded = false; + // Check whether autosave is set to load and exists + boolean loadAutosave = DirectoryManager.getAutosavedSession().exists() && PreferencesManager.getPreferences().getAsBoolean(AUTOLOAD_LAST_AUTOSAVE); + boolean genomeLoaded = false; if (igvArgs.getGenomeId() != null) { String genomeId = igvArgs.getGenomeId(); try { @@ -1948,8 +1944,8 @@ public void run() { log.error("Error loading genome: " + genomeId, e); } } - - if (genomeLoaded == false && igvArgs.getSessionFile() == null) { + // If we're not loading a session file, attempt to load a default genome file + if(igvArgs.getSessionFile() == null && !loadAutosave && !genomeLoaded) { String genomeId = preferences.getDefaultGenome(); try { GenomeManager.getInstance().loadGenomeById(genomeId); @@ -1958,19 +1954,19 @@ public void run() { MessageUtils.showErrorMessage("Error loading genome: " + genomeId, e); log.error("Error loading genome: " + genomeId, e); } - } - if (genomeLoaded == false && igvArgs.getSessionFile() == null) { - String genomeId = GenomeListManager.DEFAULT_GENOME.getId(); - try { - GenomeManager.getInstance().loadGenomeById(genomeId); - } catch (IOException e) { - MessageUtils.showErrorMessage("Error loading genome: " + genomeId, e); - log.error("Error loading genome: " + genomeId, e); + if (!genomeLoaded) { + genomeId = GenomeListManager.DEFAULT_GENOME.getId(); + try { + GenomeManager.getInstance().loadGenomeById(genomeId); + } catch (IOException e) { + MessageUtils.showErrorMessage("Error loading genome: " + genomeId, e); + log.error("Error loading genome: " + genomeId, e); + } } } - if (igvArgs.getSessionFile() != null || igvArgs.getDataFileStrings() != null) { + if (igvArgs.getSessionFile() != null || igvArgs.getDataFileStrings() != null || loadAutosave) { if (log.isDebugEnabled()) { log.debug("Loading session data"); @@ -2000,7 +1996,6 @@ public void run() { if (!success) { String genomeId = preferences.getDefaultGenome(); contentPane.getCommandBar().selectGenome(genomeId); - } } else if (igvArgs.getDataFileStrings() != null) { @@ -2067,6 +2062,15 @@ public void run() { locators.add(rl); } loadTracks(locators); + } else if (loadAutosave) { + // Get the last autosave and attempt to load + File sessionAutosave = DirectoryManager.getAutosavedSession(); + boolean success = loadSession(sessionAutosave.getAbsolutePath(), null); + // Load the default genome if unsuccessful + if (!success) { + String genomeId = preferences.getDefaultGenome(); + contentPane.getCommandBar().selectGenome(genomeId); + } } From e803e3af2d8c9ea049961dfd4628146bdde9a574 Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Thu, 4 May 2023 20:29:29 -0700 Subject: [PATCH 052/116] Range query fix (#1325) * Add some protection for unsatisfiable range queries, near the end of the file. These can arise due to buffering. Some servers do not handle these queries correctly, notably Globus. * format (code indention) -- no functional changes --- .../java/org/broad/igv/util/HttpUtils.java | 19 +++++++++-- .../util/stream/IGVSeekableHTTPStream.java | 34 +++++++++++++++---- .../org/broad/igv/util/HttpUtilsTest.java | 11 ++++++ 3 files changed, 55 insertions(+), 9 deletions(-) diff --git a/src/main/java/org/broad/igv/util/HttpUtils.java b/src/main/java/org/broad/igv/util/HttpUtils.java index 60250c434e..aebc9d8e65 100644 --- a/src/main/java/org/broad/igv/util/HttpUtils.java +++ b/src/main/java/org/broad/igv/util/HttpUtils.java @@ -198,7 +198,7 @@ public static URL createURL(String urlString) throws MalformedURLException { public static String mapURL(String urlString) throws MalformedURLException { // Check explicit mappings first - if(urlMappings.containsKey(urlString)) { + if (urlMappings.containsKey(urlString)) { return urlMappings.get(urlString); } @@ -644,6 +644,7 @@ public HttpURLConnection delete(URL url) throws IOException { return openConnection(url, Collections.emptyMap(), "DELETE"); } + // Called by IGVSeekableHTTPStream.openInputStreamForRange public HttpURLConnection openConnection(URL url, Map requestProperties) throws IOException { return openConnection(url, requestProperties, "GET"); } @@ -689,7 +690,7 @@ private HttpURLConnection openConnection( if (oauthProvider != null) { //Google is skipped here as we don't yet know if the url is protected or not. Login is invoked after 401 error - if(!oauthProvider.isGoogle()) { + if (!oauthProvider.isGoogle()) { oauthProvider.checkLogin(); } token = oauthProvider.getAccessToken(); @@ -1155,6 +1156,19 @@ public static void readFully(InputStream is, byte b[]) throws IOException { } } + /** + * Return true if URL has a known "Signed" signature. There is no expecation this is comprehensive, but it + * does match Amazon and Google patterns and possibly others* + * + * @param url + * @return + */ + public static boolean isSignedURL(String url) { + Pattern pattern = Pattern.compile("X-.*-Signature"); + Matcher matcher = pattern.matcher(url); + return matcher.find(); + } + public class UnsatisfiableRangeException extends RuntimeException { String message; @@ -1198,6 +1212,7 @@ public long getMaxAge() { } private static Map urlMappings; + static { // mutable map urlMappings = new HashMap<>(); diff --git a/src/main/java/org/broad/igv/util/stream/IGVSeekableHTTPStream.java b/src/main/java/org/broad/igv/util/stream/IGVSeekableHTTPStream.java index a72db31294..fc2e006641 100755 --- a/src/main/java/org/broad/igv/util/stream/IGVSeekableHTTPStream.java +++ b/src/main/java/org/broad/igv/util/stream/IGVSeekableHTTPStream.java @@ -26,6 +26,7 @@ package org.broad.igv.util.stream; import htsjdk.samtools.seekablestream.SeekableStream; +import org.broad.igv.exceptions.HttpResponseException; import org.broad.igv.logging.*; import org.broad.igv.util.HttpUtils; @@ -39,6 +40,7 @@ import java.util.Map; /** + * */ public class IGVSeekableHTTPStream extends SeekableStream { @@ -78,15 +80,14 @@ public void seek(final long position) { public int read(byte[] buffer, int offset, int len) throws IOException { int attempts = 0; - while(attempts < 3) { + while (attempts < 3) { try { return _read(buffer, offset, len); } catch (java.net.SocketException e) { - if(attempts < 3) { + if (attempts < 3) { attempts++; log.error("Socket exception. Trying again.", e); - } - else { + } else { throw e; } } @@ -140,9 +141,7 @@ public int _read(byte[] buffer, int offset, int len) throws IOException { } catch (HttpUtils.UnsatisfiableRangeException e) { return handleUnsatisfiableRange(n); - } - - catch (IOException e) { + } catch (IOException e) { if (e.getMessage().contains("416") || (e instanceof EOFException)) { return handleUnsatisfiableRange(n); @@ -183,6 +182,27 @@ public int read() throws IOException { public InputStream openInputStreamForRange(long start, long end) throws IOException { + // To safely do a range query we should limit it to the content length. Try to determine content-length, + // there is no guarantee this will succeed. + if (!HttpUtils.isSignedURL(url.toExternalForm())) { + try { + contentLength = HttpUtils.getInstance().getContentLength(url); + } catch (Exception e) { + log.error("Error fetching content-length", e); + } + } + + // Check content length, if known + if (contentLength > 0) { + if (start >= contentLength) { + throw new IOException("Start (" + start + ") is > content length(" + contentLength + ")"); + } else if (start == contentLength) { + + } else { + end = contentLength - 1; + } + } + String byteRange = "bytes=" + start + "-" + end; Map params = new HashMap(); params.put("Range", byteRange); diff --git a/src/test/java/org/broad/igv/util/HttpUtilsTest.java b/src/test/java/org/broad/igv/util/HttpUtilsTest.java index 270936c454..7961e7fa48 100644 --- a/src/test/java/org/broad/igv/util/HttpUtilsTest.java +++ b/src/test/java/org/broad/igv/util/HttpUtilsTest.java @@ -49,6 +49,17 @@ public class HttpUtilsTest extends AbstractHeadlessTest { static String broadURLString = "https://igv-genepattern-org.s3.amazonaws.com/test/fasta/chr22.fa"; + @Test + public void testSignedURLMatch() throws Exception { + String aws = "https://amazonaws.com?X-Amz-Signature=foo"; //X-Amz-Signature" + assertTrue(HttpUtils.isSignedURL(aws)); + String google = "https://google.com?X-Goog-Signature=bar"; + assertTrue(HttpUtils.isSignedURL(google)); + String noMatch = "https://www.google.com"; + assertFalse(HttpUtils.isSignedURL(noMatch)); + } + + @Test public void testGetContentLength() throws IOException { // Open an input stream just to check permissions From ce1d22c48f2a8dfbcf54005732214461cede26cb Mon Sep 17 00:00:00 2001 From: kcibul Date: Fri, 19 May 2023 17:56:10 -0400 Subject: [PATCH 053/116] Add AWSSDK SSO Dependency (#1334) --- build.gradle | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/build.gradle b/build.gradle index 0adbbfc315..f4d18a57e3 100644 --- a/build.gradle +++ b/build.gradle @@ -123,7 +123,8 @@ dependencies { // Amazon deps [group: 'software.amazon.awssdk', name: 'cognitoidentity', version: '2.16.7'], [group: 'software.amazon.awssdk', name: 'sts', version: '2.16.7'], - [group: 'software.amazon.awssdk', name: 's3', version: '2.16.7'] + [group: 'software.amazon.awssdk', name: 's3', version: '2.16.7'], + [group: 'software.amazon.awssdk', name: 'sso', version: '2.16.7'] ) testImplementation( From 84257e0d93769519b2f488a26860be96b3f72d17 Mon Sep 17 00:00:00 2001 From: Kevin Lydon Date: Thu, 1 Jun 2023 01:18:06 -0400 Subject: [PATCH 054/116] Timed autosaves of sessions (#1327) * Added some preferences for configuring a timed autosave, which is triggered with a Timer Added a check in the recent session list loading to remove sessions that don't exist anymore * Now the autosaves created on exit will not be deleted as part of the periodic autosave functionality, and there is a submenu of the file menu that populates with current autosave files * Saving a session will now fill in the default session name when saving a session loaded from an autosave (instead of the filename of the autosave). Also changed the default for AUTOSAVE_FREQUENCY to 0 so timed autosave is disabled by default, and added clarity to the description for that option so the user knows that setting it to 0 will disable it * Added an option to disable the autosave-on-exit. Changed the exit autosave session file name to be more clear. Changed the organization of the load autosave menu to have a separator between the exit autosave and the timed autosaves and to sort the autosaves by date descending. Fixed an issue where autoloading on startup would fail if there were only timed autosaves. --- .../java/org/broad/igv/DirectoryManager.java | 43 +++---- .../java/org/broad/igv/prefs/Constants.java | 3 + .../session/autosave/AutosaveTimerTask.java | 50 ++++++++ .../autosave/SessionAutosaveManager.java | 119 ++++++++++++++++++ src/main/java/org/broad/igv/ui/IGV.java | 63 ++++++++-- .../java/org/broad/igv/ui/IGVMenuBar.java | 7 +- .../igv/ui/action/SaveSessionMenuAction.java | 9 +- .../org/broad/igv/ui/util/AutosaveMenu.java | 73 +++++++++++ .../org/broad/igv/prefs/defaults_2.3.tab | 3 + .../org/broad/igv/prefs/preferences.tab | 9 +- 10 files changed, 338 insertions(+), 41 deletions(-) create mode 100644 src/main/java/org/broad/igv/session/autosave/AutosaveTimerTask.java create mode 100644 src/main/java/org/broad/igv/session/autosave/SessionAutosaveManager.java create mode 100644 src/main/java/org/broad/igv/ui/util/AutosaveMenu.java diff --git a/src/main/java/org/broad/igv/DirectoryManager.java b/src/main/java/org/broad/igv/DirectoryManager.java index 316efff4f0..5d7c98a5e1 100644 --- a/src/main/java/org/broad/igv/DirectoryManager.java +++ b/src/main/java/org/broad/igv/DirectoryManager.java @@ -54,6 +54,7 @@ public class DirectoryManager { private static File USER_HOME; private static File USER_DIRECTORY; // FileSystemView.getFileSystemView().getDefaultDirectory(); private static File IGV_DIRECTORY; // The IGV application directory + private static File AUTOSAVE_DIRECTORY; private static File GENOME_CACHE_DIRECTORY; private static File GENE_LIST_DIRECTORY; private static File BAM_CACHE_DIRECTORY; @@ -179,6 +180,21 @@ private static File getIgvDirectoryOverride() { return override; } + public static File getAutosaveDirectory() { + if (AUTOSAVE_DIRECTORY == null) { + + AUTOSAVE_DIRECTORY = new File(getIgvDirectory(), "autosave"); + if (!AUTOSAVE_DIRECTORY.exists()) { + AUTOSAVE_DIRECTORY.mkdir(); + } + if (!AUTOSAVE_DIRECTORY.canRead()) { + throw new DataLoadException("Cannot read from user directory", AUTOSAVE_DIRECTORY.getAbsolutePath()); + } else if (!AUTOSAVE_DIRECTORY.canWrite()) { + throw new DataLoadException("Cannot write to user directory", AUTOSAVE_DIRECTORY.getAbsolutePath()); + } + } + return AUTOSAVE_DIRECTORY; + } public static File getGenomeCacheDirectory() { if (GENOME_CACHE_DIRECTORY == null) { @@ -263,32 +279,6 @@ public static synchronized File getLogFile() throws IOException { } - /** - * Creates and returns a file object for the file where a user's session is autosaved on exit. - * - * @return File object for the session autosave file - */ - public static File getAutosavedSession() { - File autosavedSessionFile = new File(getIgvDirectory(), "session_autosave.xml"); - return autosavedSessionFile; - } - - /** - * Creates and returns a file object for the file where a user's session is autosaved on exit. - * If the file does not exist in the file system (at "~/igv/session_autosave.xml"), creates it. - * - * @return File object for the session autosave file - * @throws IOException - */ - public static synchronized File getAutosavedSessionCreateIfNotExists() throws IOException { - - File autosavedSessionFile = new File(getIgvDirectory(), "session_autosave.xml"); - if (!autosavedSessionFile.exists()) { - autosavedSessionFile.createNewFile(); - } - return autosavedSessionFile; - } - /** * Return the user preferences property file ("~/igv/prefs.properties"). @@ -374,6 +364,7 @@ public static void moveIGVDirectory(final File newIGVDirectoryParent) { } + AUTOSAVE_DIRECTORY = null; GENOME_CACHE_DIRECTORY = null; GENE_LIST_DIRECTORY = null; BAM_CACHE_DIRECTORY = null; diff --git a/src/main/java/org/broad/igv/prefs/Constants.java b/src/main/java/org/broad/igv/prefs/Constants.java index c38c29818c..8b320a968f 100644 --- a/src/main/java/org/broad/igv/prefs/Constants.java +++ b/src/main/java/org/broad/igv/prefs/Constants.java @@ -34,7 +34,10 @@ private Constants() { public static final String SHOW_GENOME_SERVER_WARNING = "SHOW_GENOME_SERVER_WARNING"; public static final String CN_FREQ_AMP_THRESHOLD = "CN_FREQ.AMP_THRESHOLD"; public static final String CN_FREQ_DEL_THRESHOLD = "CN_FREQ.DEL_THRESHOLD"; + public static final String AUTOSAVE_ON_EXIT = "AUTOSAVE_ON_EXIT"; public static final String AUTOLOAD_LAST_AUTOSAVE = "AUTOLOAD_LAST_AUTOSAVE"; + public static final String AUTOSAVE_FREQUENCY = "AUTOSAVE_FREQUENCY"; + public static final String AUTOSAVES_TO_KEEP = "AUTOSAVES_TO_KEEP"; // public static final String RECENT_SESSIONS = "IGV.Session.recent.sessions"; diff --git a/src/main/java/org/broad/igv/session/autosave/AutosaveTimerTask.java b/src/main/java/org/broad/igv/session/autosave/AutosaveTimerTask.java new file mode 100644 index 0000000000..c213415670 --- /dev/null +++ b/src/main/java/org/broad/igv/session/autosave/AutosaveTimerTask.java @@ -0,0 +1,50 @@ +package org.broad.igv.session.autosave; + +import org.broad.igv.logging.LogManager; +import org.broad.igv.logging.Logger; +import org.broad.igv.session.Session; +import org.broad.igv.ui.IGV; +import org.broad.igv.ui.util.MessageUtils; + +import java.io.IOException; +import java.util.TimerTask; + +/** + * Triggers a session autosave when dictated by a timer + */ +public class AutosaveTimerTask extends TimerTask { + + private static Logger log = LogManager.getLogger(AutosaveTimerTask.class); + + private IGV igv; + + /** + * Creates a new task which will save the current session for the specified IGV + * instance when triggered by a timer + * @param igv We'll autosave the session from this IGV instance + */ + public AutosaveTimerTask(IGV igv) { + this.igv = igv; + } + + /** + * Saves the current session for this listener's IGV instance + */ + @Override + public void run() { + // Get the current session so we can save it + Session session = igv.getSession(); + try { + // Save the session to a new file in the autosave directory + SessionAutosaveManager.saveTimedSessionAutosaveFile(session); + } + catch (IOException err) { + // If autosaving fails, notify the user and stop autosaving + final String message = "Failure while trying to autosave session. Timed autosave will be disabled until IGV is restarted."; + log.error(message, err); + MessageUtils.showMessage(message + "\n" + err); + igv.stopTimedAutosave(); + } + + } +} diff --git a/src/main/java/org/broad/igv/session/autosave/SessionAutosaveManager.java b/src/main/java/org/broad/igv/session/autosave/SessionAutosaveManager.java new file mode 100644 index 0000000000..0d17bc28f4 --- /dev/null +++ b/src/main/java/org/broad/igv/session/autosave/SessionAutosaveManager.java @@ -0,0 +1,119 @@ +package org.broad.igv.session.autosave; + +import org.broad.igv.DirectoryManager; +import org.broad.igv.prefs.Constants; +import org.broad.igv.prefs.PreferencesManager; +import org.broad.igv.session.Session; +import org.broad.igv.session.SessionWriter; + +import java.io.File; +import java.io.IOException; +import java.nio.file.Files; +import java.nio.file.attribute.BasicFileAttributes; +import java.nio.file.attribute.FileTime; +import java.text.SimpleDateFormat; +import java.util.*; +import java.util.stream.Collectors; + +/** + * Defines several static methods for saving and retrieving session autosave files + */ +public class SessionAutosaveManager { + + + /** + * Returns a file object for the file corresponding to the autosave-on-exit session file, or an empty optional if + * the file does not exist + * + * @return Optional containing a File object for the session autosave file or empty + */ + public static synchronized Optional getExitSessionAutosaveFile() { + File autosavedSessionFile = new File(DirectoryManager.getAutosaveDirectory(), "exit_session_autosave.xml"); + if(!autosavedSessionFile.exists()) { + return Optional.empty(); + } + return Optional.of(autosavedSessionFile); + } + + /** + * Saves the specified session to a file named to indicate it is an autosave. Creates the file if one does not yet + * exist, or overwrites an existing session autosave created on exit + * + * @param session the session to save + * @throws IOException if saving the session fails + */ + public static synchronized void saveExitSessionAutosaveFile(Session session) throws IOException { + // Get the file we use for saving sessions on exit + File autosavedSessionFile = new File(DirectoryManager.getAutosaveDirectory(), "exit_session_autosave.xml"); + // Save the session + (new SessionWriter()).saveSession(session, autosavedSessionFile); + } + + /** + * Gets a File object for each timed autosave session file in the autosave directory and returns an array of those + * objects + * + * @return Array of autosave files + */ + public static synchronized File[] getTimedSessionAutosaveFiles() { + File[] autosaveFiles = DirectoryManager.getAutosaveDirectory().listFiles(); + // Remove the exit session autosave file from the list + return Arrays.stream(autosaveFiles) + .filter(file -> !file.getName().equals("exit_session_autosave.xml")) + .toArray(File[]::new); + } + + /** + * Creates a new timed autosave session file and writes the provided session to it + * + * @param session The session to save + * @throws IOException if saving the session or deleting sessions over the limit fails + */ + public static synchronized void saveTimedSessionAutosaveFile(Session session) throws IOException { + // Create a filename for the new autosave file that includes the current datetime + String currentDate = new SimpleDateFormat("yyyy-MM-dd'T'HH:mm:ss.SSS'Z'").format(new Date()); + String newAutosaveFilename = "session_autosave" + currentDate + ".xml"; + + // Create the file + File autosavedSessionFile = new File(DirectoryManager.getAutosaveDirectory(), newAutosaveFilename); + autosavedSessionFile.createNewFile(); + + // If this puts the number of timed autosave files over the maximum, delete the oldest (the first in + // alphabetical order that is the one created on exit) + File[] autosaves = getTimedSessionAutosaveFiles(); + if(autosaves.length > PreferencesManager.getPreferences().getAsInt(Constants.AUTOSAVES_TO_KEEP)) { + Arrays.sort(autosaves); + autosaves[0].delete(); + } + + // Save the session + (new SessionWriter()).saveSession(session, autosavedSessionFile); + } + + /** + * Gets a File object for the most recent file in the autosave directory, or an empty optional if the directory is + * empty + * + * @return Optional containing a File object for the session autosave file or empty + */ + public static synchronized Optional getMostRecentAutosaveFile() throws IOException{ + File[] autosaveFiles = DirectoryManager.getAutosaveDirectory().listFiles(); + if(autosaveFiles.length < 1) { + return Optional.empty(); + } + // Check the creation dates of the files so we can figure out which is the most recent + int latestIndex = 0; + long latestCreationTime = Files.readAttributes(autosaveFiles[0].toPath(), BasicFileAttributes.class) + .creationTime().toMillis(); + for(int i = 1; i < autosaveFiles.length; i++) { + // This file is the new latest if its creationTime is later than the current latest + long currentCreationTime = Files.readAttributes(autosaveFiles[i].toPath(), BasicFileAttributes.class) + .creationTime().toMillis(); + if(currentCreationTime > latestCreationTime) { + latestCreationTime = currentCreationTime; + latestIndex = i; + } + } + return Optional.of(autosaveFiles[latestIndex]); + } +} diff --git a/src/main/java/org/broad/igv/ui/IGV.java b/src/main/java/org/broad/igv/ui/IGV.java index 1acf776cd5..0f68aad6ee 100644 --- a/src/main/java/org/broad/igv/ui/IGV.java +++ b/src/main/java/org/broad/igv/ui/IGV.java @@ -51,6 +51,7 @@ import org.broad.igv.lists.GeneList; import org.broad.igv.logging.LogManager; import org.broad.igv.logging.Logger; +import org.broad.igv.prefs.Constants; import org.broad.igv.prefs.IGVPreferences; import org.broad.igv.prefs.PreferencesEditor; import org.broad.igv.prefs.PreferencesManager; @@ -58,6 +59,8 @@ import org.broad.igv.sam.InsertionSelectionEvent; import org.broad.igv.sam.SortOption; import org.broad.igv.session.*; +import org.broad.igv.session.autosave.AutosaveTimerTask; +import org.broad.igv.session.autosave.SessionAutosaveManager; import org.broad.igv.track.*; import org.broad.igv.ui.WaitCursorManager.CursorToken; import org.broad.igv.ui.commandbar.GenomeListManager; @@ -81,6 +84,7 @@ import java.net.URL; import java.util.List; import java.util.*; +import java.util.Timer; import java.util.concurrent.CompletableFuture; import java.util.concurrent.ExecutorService; import java.util.concurrent.Executors; @@ -126,6 +130,11 @@ public class IGV implements IGVEventObserver { */ private Session session; + /** + * Timer for triggering periodic autosave of current session + */ + private Timer sessionAutosaveTimer = new Timer(); + // Misc state private Map> overlayTracksMap = new HashMap(); private Set overlaidTracks = new HashSet(); @@ -249,6 +258,12 @@ public void windowGainedFocus(WindowEvent windowEvent) { mainFrame.setBounds(applicationBounds); subscribeToEvents(); + + // Start running periodic autosaves (unless the user has specified not to retain timed autosaves) + if(PreferencesManager.getPreferences().getAsInt(Constants.AUTOSAVES_TO_KEEP) > 0) { + int timerDelay = PreferencesManager.getPreferences().getAsInt(AUTOSAVE_FREQUENCY) * 60000; // Convert timer delay to ms + sessionAutosaveTimer.scheduleAtFixedRate(new AutosaveTimerTask(this), timerDelay, timerDelay); + } } public JRootPane getRootPane() { @@ -562,15 +577,20 @@ final public void saveStateForExit() { PreferencesManager.getPreferences().setRecentSessions(recentSessions); } - // Autosave current session - try { - File sessionAutosave = DirectoryManager.getAutosavedSessionCreateIfNotExists(); - saveSession(sessionAutosave); - } - catch(Exception e) { - log.error("Error autosaving session", e); + // Stop the timer that is triggering the timed autosave + this.stopTimedAutosave(); + + // Autosave current session if configured to do so + if(PreferencesManager.getPreferences().getAsBoolean(AUTOSAVE_ON_EXIT)) { + try { + SessionAutosaveManager.saveExitSessionAutosaveFile(session); + } + catch(Exception e) { + log.error("Error autosaving session", e); + } } + } final public void doShowAttributeDisplay(boolean enableAttributeView) { @@ -1931,7 +1951,14 @@ public void run() { } else { // Check whether autosave is set to load and exists - boolean loadAutosave = DirectoryManager.getAutosavedSession().exists() && PreferencesManager.getPreferences().getAsBoolean(AUTOLOAD_LAST_AUTOSAVE); + boolean autosavePresent = false; + try { + autosavePresent = SessionAutosaveManager.getMostRecentAutosaveFile().isPresent(); + } + catch(Exception e) { + log.error("Failure trying to get most recent autosave file", e); + } + boolean loadAutosave = autosavePresent && PreferencesManager.getPreferences().getAsBoolean(AUTOLOAD_LAST_AUTOSAVE); boolean genomeLoaded = false; if (igvArgs.getGenomeId() != null) { @@ -2063,9 +2090,16 @@ public void run() { } loadTracks(locators); } else if (loadAutosave) { - // Get the last autosave and attempt to load - File sessionAutosave = DirectoryManager.getAutosavedSession(); - boolean success = loadSession(sessionAutosave.getAbsolutePath(), null); + boolean success = false; + try { + // Get the last autosave and attempt to load + File sessionAutosave = SessionAutosaveManager.getMostRecentAutosaveFile().get(); + success = loadSession(sessionAutosave.getAbsolutePath(), null); + } + catch(Exception e) { + log.error("Failure trying to load most recent autosave file", e); + } + // Load the default genome if unsuccessful if (!success) { String genomeId = preferences.getDefaultGenome(); @@ -2344,6 +2378,13 @@ public List visibleTracks(DataPanelContainer dataPanelContainer) { collect(Collectors.toList()); } + /** + * Stops the scheduled autosave task + */ + public void stopTimedAutosave() { + sessionAutosaveTimer.cancel(); + } + // Thread pool for loading data private static final ExecutorService threadExecutor = Executors.newFixedThreadPool(5); } diff --git a/src/main/java/org/broad/igv/ui/IGVMenuBar.java b/src/main/java/org/broad/igv/ui/IGVMenuBar.java index 42cd405f03..aca257bf9f 100644 --- a/src/main/java/org/broad/igv/ui/IGVMenuBar.java +++ b/src/main/java/org/broad/igv/ui/IGVMenuBar.java @@ -97,6 +97,7 @@ public class IGVMenuBar extends JMenuBar implements IGVEventObserver { private JMenu toolsMenu; private JMenu googleMenu; private JMenu AWSMenu; + private AutosaveMenu autosaveMenu; private FilterTracksMenuAction filterTracksAction; private JMenu viewMenu; private IGV igv; @@ -322,6 +323,9 @@ JMenu createFileMenu() { reloadSessionItem.setEnabled(false); menuItems.add(reloadSessionItem); + autosaveMenu = new AutosaveMenu(); + menuItems.add(autosaveMenu); + menuItems.add(new JSeparator()); // ***** Snapshots @@ -375,7 +379,8 @@ public void actionPerformed(ActionEvent e) { String[] sessions = recentSessions.split(";"); for (String sessionPath : sessions) { if (!sessionPath.equals("null") && - !igv.getRecentSessionList().contains(sessionPath)) { + !igv.getRecentSessionList().contains(sessionPath) && + (new File(sessionPath)).exists()) { igv.getRecentSessionList().add(sessionPath); } diff --git a/src/main/java/org/broad/igv/ui/action/SaveSessionMenuAction.java b/src/main/java/org/broad/igv/ui/action/SaveSessionMenuAction.java index b6a415b3c1..1d1a732b7d 100644 --- a/src/main/java/org/broad/igv/ui/action/SaveSessionMenuAction.java +++ b/src/main/java/org/broad/igv/ui/action/SaveSessionMenuAction.java @@ -31,7 +31,9 @@ //~--- non-JDK imports -------------------------------------------------------- +import org.broad.igv.DirectoryManager; import org.broad.igv.logging.*; +import org.broad.igv.prefs.Constants; import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.session.Session; import org.broad.igv.session.SessionWriter; @@ -78,7 +80,12 @@ public void actionPerformed(ActionEvent e) { String currentSessionFilePath = igv.getSession().getPath(); - String initFile = currentSessionFilePath == null ? UIConstants.DEFAULT_SESSION_FILE : currentSessionFilePath; + // Get the parent dir of the session file so we can check if it's in the autosave directory + File parentDir = currentSessionFilePath == null ? null : new File(new File(currentSessionFilePath).getParent()); + + // If the filepath is null or the file is in the autosave dir, use the default session file name + String initFile = currentSessionFilePath == null || parentDir.equals(DirectoryManager.getAutosaveDirectory()) ? + UIConstants.DEFAULT_SESSION_FILE : currentSessionFilePath; sessionFile = FileDialogUtils.chooseFile("Save Session", PreferencesManager.getPreferences().getLastTrackDirectory(), new File(initFile), diff --git a/src/main/java/org/broad/igv/ui/util/AutosaveMenu.java b/src/main/java/org/broad/igv/ui/util/AutosaveMenu.java new file mode 100644 index 0000000000..b6069c9264 --- /dev/null +++ b/src/main/java/org/broad/igv/ui/util/AutosaveMenu.java @@ -0,0 +1,73 @@ +package org.broad.igv.ui.util; + +import org.broad.igv.session.autosave.SessionAutosaveManager; +import org.broad.igv.ui.IGV; +import org.broad.igv.ui.action.OpenSessionMenuAction; + +import javax.swing.*; +import javax.swing.event.MenuEvent; +import javax.swing.event.MenuListener; +import java.io.File; +import java.util.Arrays; +import java.util.Collections; +import java.util.Optional; + +/** + * A menu for listing currently available autosaved sessions that can be opened + */ +public class AutosaveMenu extends JMenu { + + public AutosaveMenu() { + this("Autosaved Sessions"); + } + + private AutosaveMenu(String name) { + super(name); + + this.addMenuListener(new MenuListener() { + @Override + public void menuSelected(MenuEvent e) { + fillAutosaveList(); + } + + @Override + public void menuDeselected(MenuEvent e) { + + } + + @Override + public void menuCanceled(MenuEvent e) { + + } + }); + } + + /** + * Fills this menu with options for opening each of the files in the autosave directory + */ + private void fillAutosaveList() { + // Get the exit session autosave file + Optional exitAutosave = SessionAutosaveManager.getExitSessionAutosaveFile(); + // Get a current list of timed autosaves + File[] timedAutosaves = SessionAutosaveManager.getTimedSessionAutosaveFiles(); + Arrays.sort(timedAutosaves, Collections.reverseOrder()); + // Remove what's in the menu right now + this.removeAll(); + // Add the exit session autosave file if it exists + if(exitAutosave.isPresent()) { + add(MenuAndToolbarUtils.createMenuItem( + new OpenSessionMenuAction(exitAutosave.get().getAbsolutePath(), IGV.getInstance()) + )); + // Separate this from the timed autosaves + if(timedAutosaves.length > 0) { + addSeparator(); + } + } + // Create a menu item for each of the timed autosave files and add it to the menu + for(int i = 0; i < timedAutosaves.length; i++) { + add(MenuAndToolbarUtils.createMenuItem( + new OpenSessionMenuAction(timedAutosaves[i].getAbsolutePath(), IGV.getInstance()) + )); + } + } +} diff --git a/src/main/resources/org/broad/igv/prefs/defaults_2.3.tab b/src/main/resources/org/broad/igv/prefs/defaults_2.3.tab index 004a16a36b..223f6e152f 100644 --- a/src/main/resources/org/broad/igv/prefs/defaults_2.3.tab +++ b/src/main/resources/org/broad/igv/prefs/defaults_2.3.tab @@ -2,6 +2,9 @@ AF_REF.COLOR 0,0,220 AF_VAR.COLOR 255,0,0 AUTO_UPDATE_GENOMES true AUTOLOAD_LAST_AUTOSAVE false +AUTOSAVE_FREQUENCY 10 +AUTOSAVE_ON_EXIT true +AUTOSAVES_TO_KEEP 0 BACKGROUND_COLOR 250,250,250 BLAT_URL http://genome.cse.ucsc.edu/cgi-bin/hgBlat BYPASS_FILE_AUTO_DISCOVERY false diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index 88b9cc1613..8ce9bd3cff 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -1,13 +1,18 @@ #General SESSION.RELATIVE_PATH Use relative paths in session files boolean TRUE -AUTOLOAD_LAST_AUTOSAVE Load last autosaved session on startup boolean FALSE IGV.single.track.pane Display all tracks in a single panel boolean FALSE IGV.track.show.attribute.views Show attribute panel boolean FALSE SHOW_DEFAULT_TRACK_ATTRIBUTES Show default track attributes boolean FALSE SHOW_REGION_BARS Show region-of-interest boundaries boolean FALSE ENABLE_GOOGLE_MENU Enable Google access boolean FALSE Enable loading from Google apis. FLANKING_REGION "Search for feature" padding region in bp, < 0 is interpreted as a percentage. integer 2000 -DEFAULT_VISIBILITY_WINDOW Default visibility window (kilobases), < 0 disables visibility window. float -1 +DEFAULT_VISIBILITY_WINDOW Default visibility window (kilobases), < 0 disables visibility window. float -1 + +## Session Autosave +AUTOSAVE_ON_EXIT Automatically save session on exit boolean TRUE +AUTOLOAD_LAST_AUTOSAVE Load last autosaved session on startup boolean FALSE +AUTOSAVE_FREQUENCY How often, in minutes, to autosave the current session integer 10 +AUTOSAVES_TO_KEEP How many timed autosave session files to keep (0 disables timed autosave) integer 0 ##Jump To Next Feature Options (f/b Keys) NEXT_FIT_TO_WINDOW Fit feature to window (with optional padding). boolean FALSE From fa4601642f287c0a542c6c41460856ad72d394cf Mon Sep 17 00:00:00 2001 From: Louis Bergelson Date: Thu, 1 Jun 2023 01:18:56 -0400 Subject: [PATCH 055/116] Adding improved support for displaying chimeric / supplementary reads (#1331) * Adding improved support for displaying chimeric / supplementary reads * When reads include an SA tag it will now be used to add additional information Color clipped ends by the associated supplementary reads location * within a chromosome color by inversion/non-inversion * connections between chromosomes are colored by the associated chromosome * new preference to display the nane the connected chromosome * new grouping option to group reads that have an SA tag vs not * new sort options to sort reads by the amount of clipping at either end * New diagram popup to visually show the connections between a single reads set of supplementary reads * Improved supplementary read information in the tooltip * Splitting by SATag / Mates now displays the selected reads at the top of the window * fixed some issues around frame loading to support this where each frame would force a full repack of all the other frames when it finished loading * Other changes and additions: * Added a new ClippingCounts class to standarize how clipping information is counted and passed around * Many additions to SupplementaryAlignment * Changes to Alignment and SAMAlignment to support more efficient CIGAR access and caching of expensive computed values * Select read by name now prefils the name of the clicked read if there is one * Fix frame sorting issue * Improve frame loading so refreshing a single frame doesn't force a repack of every frame. * Reactived event that fires on data load which is used to complete sorts that * Triggering a sort when all frames are not fully loaded will now queue the sort and perform it when the frame is done loading * make STOP button work to abort loading reads, it may end up in a weird state, but it's the same state you get when it stops itself due to memory issues, this just lets you get there without waiting for memory to fill up * delete unused RenderReset class --- build.gradle | 2 +- .../org/broad/igv/batch/CommandExecutor.java | 2 +- .../java/org/broad/igv/feature/Strand.java | 13 +- .../java/org/broad/igv/jbrowse/Chord.java | 10 +- .../igv/jbrowse/CircularViewUtilities.java | 3 +- .../java/org/broad/igv/prefs/Constants.java | 2 + .../java/org/broad/igv/sam/Alignment.java | 34 ++- .../broad/igv/sam/AlignmentDataManager.java | 9 +- .../org/broad/igv/sam/AlignmentInterval.java | 11 +- .../org/broad/igv/sam/AlignmentPacker.java | 22 +- .../org/broad/igv/sam/AlignmentRenderer.java | 185 +++++++++--- .../broad/igv/sam/AlignmentTileLoader.java | 3 +- .../org/broad/igv/sam/AlignmentTrack.java | 73 ++--- .../org/broad/igv/sam/AlignmentTrackMenu.java | 87 ++++-- .../org/broad/igv/sam/ClippingCounts.java | 139 +++++++++ .../java/org/broad/igv/sam/CoverageTrack.java | 3 - .../broad/igv/sam/DotAlignedAlignment.java | 33 --- .../igv/sam/FeatureWrappedAlignment.java | 28 -- .../org/broad/igv/sam/LinkedAlignment.java | 22 -- .../org/broad/igv/sam/PairedAlignment.java | 22 +- .../broad/igv/sam/ReducedMemoryAlignment.java | 28 -- .../java/org/broad/igv/sam/SAMAlignment.java | 175 ++++++----- .../java/org/broad/igv/sam/SortOption.java | 25 ++ .../broad/igv/sam/SupplementaryAlignment.java | 274 +++++++++++++++--- .../broad/igv/ui/AlignmentDiagramFrame.java | 234 +++++++++++++++ src/main/java/org/broad/igv/ui/IGV.java | 19 +- .../org/broad/igv/ui/panel/DataPanel.java | 2 +- .../org/broad/igv/ui/panel/FrameManager.java | 3 +- .../igv/ui/util/ApplicationStatusBar.java | 5 + .../org/broad/igv/ui/util/UIUtilities.java | 5 +- .../org/broad/igv/util/ChromosomeColors.java | 2 +- .../org/broad/igv/prefs/preferences.tab | 1 + .../broad/igv/sam/BisulfiteBaseInfoTest.java | 20 -- .../org/broad/igv/sam/ClippingCountsTest.java | 53 ++++ 34 files changed, 1127 insertions(+), 422 deletions(-) create mode 100644 src/main/java/org/broad/igv/sam/ClippingCounts.java create mode 100644 src/main/java/org/broad/igv/ui/AlignmentDiagramFrame.java create mode 100644 src/test/java/org/broad/igv/sam/ClippingCountsTest.java diff --git a/build.gradle b/build.gradle index f4d18a57e3..0f2a391c25 100644 --- a/build.gradle +++ b/build.gradle @@ -1,6 +1,6 @@ // The MIT License (MIT) // -// Copyright (c) 2007-2019 Broad Institute +// Copyright (c) 2007-2019 Broad Institut≈e // // Permission is hereby granted, free of charge, to any person obtaining a copy // of this software and associated documentation files (the "Software"), to deal diff --git a/src/main/java/org/broad/igv/batch/CommandExecutor.java b/src/main/java/org/broad/igv/batch/CommandExecutor.java index 293e7dba21..eaae030f07 100755 --- a/src/main/java/org/broad/igv/batch/CommandExecutor.java +++ b/src/main/java/org/broad/igv/batch/CommandExecutor.java @@ -1082,7 +1082,7 @@ private String sort(String sortArg, String param2, String param3, String param4) boolean invertSort = "reverse".equalsIgnoreCase(reverseString); - igv.sortAlignmentTracks(getAlignmentSortOption(sortArg), location, tag, invertSort); + igv.sortAlignmentTracks(getAlignmentSortOption(sortArg), location, tag, invertSort, null); return "OK"; } } diff --git a/src/main/java/org/broad/igv/feature/Strand.java b/src/main/java/org/broad/igv/feature/Strand.java index d0f2299010..d07d9f2180 100644 --- a/src/main/java/org/broad/igv/feature/Strand.java +++ b/src/main/java/org/broad/igv/feature/Strand.java @@ -34,11 +34,22 @@ public enum Strand { - POSITIVE, NEGATIVE, NONE; //The order here matters because it sets how strand sorts. + POSITIVE("+"), + NEGATIVE("-"), + NONE(""); //The order here matters because it sets how strand sorts. + private final String shortString; + + private Strand(String shortString){ + this.shortString = shortString; + } public static Strand fromString(String strandString) { return strandString.equals("+") || strandString.equalsIgnoreCase("POSITIVE") ? POSITIVE : (strandString.equals("-") || strandString.equalsIgnoreCase("NEGATIVE") ? NEGATIVE : NONE); } + + public String toShortString(){ + return shortString; + } } diff --git a/src/main/java/org/broad/igv/jbrowse/Chord.java b/src/main/java/org/broad/igv/jbrowse/Chord.java index 1f58d4977a..36c1a8c15b 100644 --- a/src/main/java/org/broad/igv/jbrowse/Chord.java +++ b/src/main/java/org/broad/igv/jbrowse/Chord.java @@ -1,5 +1,6 @@ package org.broad.igv.jbrowse; +import htsjdk.samtools.SAMTag; import org.broad.igv.Globals; import org.broad.igv.bedpe.BedPE; import org.broad.igv.bedpe.BedPEFeature; @@ -51,21 +52,20 @@ public static Chord fromPEAlignment(Alignment a) { return c; } - public static List fromSAString(Alignment a) { - - String sastring = a.getAttribute("SA").toString(); + public static List fromSAString(Alignment a) { + String sastring = a.getAttribute(SAMTag.SA.name()).toString(); String[] records = Globals.semicolonPattern.split(sastring); List chords = new ArrayList<>(); int n = 0; for (String rec : records) { - SupplementaryAlignment sa = new SupplementaryAlignment(rec); + SupplementaryAlignment sa = SupplementaryAlignment.fromSingleSaTagRecord(rec); if (sa.start != a.getStart()) { Chord c = new Chord(); c.uniqueId = a.getReadName() + "_" + n++; c.refName = shortName(a.getChr()); c.start = a.getStart(); c.end = a.getEnd(); - c.mate = new Mate(shortName(sa.chr), sa.start, sa.start + sa.lenOnRef); + c.mate = new Mate(shortName(sa.chr), sa.start, sa.start + sa.getLenOnRef()); chords.add(c); } } diff --git a/src/main/java/org/broad/igv/jbrowse/CircularViewUtilities.java b/src/main/java/org/broad/igv/jbrowse/CircularViewUtilities.java index 2d509f5ebf..9c00d769e6 100644 --- a/src/main/java/org/broad/igv/jbrowse/CircularViewUtilities.java +++ b/src/main/java/org/broad/igv/jbrowse/CircularViewUtilities.java @@ -1,5 +1,6 @@ package org.broad.igv.jbrowse; +import htsjdk.samtools.SAMTag; import htsjdk.tribble.Feature; import org.broad.igv.bedpe.BedPE; import org.broad.igv.feature.Chromosome; @@ -45,7 +46,7 @@ public static void sendAlignmentsToJBrowse(List alignments, String tr if (a.isPaired() && a.getMate().isMapped()) { chords.add(Chord.fromPEAlignment(a)); } - if (a.getAttribute("SA") != null) { + if (a.getAttribute(SAMTag.SA.name()) != null) { chords.addAll(Chord.fromSAString(a)); } } diff --git a/src/main/java/org/broad/igv/prefs/Constants.java b/src/main/java/org/broad/igv/prefs/Constants.java index 8b320a968f..594678926f 100644 --- a/src/main/java/org/broad/igv/prefs/Constants.java +++ b/src/main/java/org/broad/igv/prefs/Constants.java @@ -167,6 +167,8 @@ private Constants() { public static final String SAM_LARGE_INDELS_THRESHOLD = "SAM.LARGE_INSERTIONS_THRESOLD"; public static final String SAM_FLAG_CLIPPING = "SAM.FLAG_CLIPPING"; public static final String SAM_CLIPPING_THRESHOLD = "SAM.CLIPPING_THRESHOLD"; + + public static final String SAM_SHOW_CONNECTED_CHR_NAME = "SAM.SHOW_CONNECTED_CHR_NAME"; public static final String SAM_SHOW_GROUP_SEPARATOR = "SAM.SHOW_GROUP_SEPARATOR"; public static final String SAM_REDUCED_MEMORY_MODE = "SAM.REDUCED_MEMORY_MODE"; public static final String SAM_HIDE_SMALL_INDEL = "SAM.HIDE_SMALL_INDEL"; diff --git a/src/main/java/org/broad/igv/sam/Alignment.java b/src/main/java/org/broad/igv/sam/Alignment.java index 872cac0577..9442bbe5df 100644 --- a/src/main/java/org/broad/igv/sam/Alignment.java +++ b/src/main/java/org/broad/igv/sam/Alignment.java @@ -29,6 +29,7 @@ */ package org.broad.igv.sam; +import htsjdk.samtools.Cigar; import org.broad.igv.feature.LocusScore; import org.broad.igv.feature.Strand; import org.broad.igv.sam.mods.BaseModificationUtils; @@ -57,7 +58,7 @@ default String getReadSequence() { return ""; } - String getChr(); + default String getChr(){ return getContig();} int getAlignmentStart(); @@ -69,9 +70,16 @@ default String getReadSequence() { AlignmentBlock[] getInsertions(); - String getCigarString(); + /** + * @return the CIGAR string of the alignment if present, otherwise "*", should not return null + */ + default String getCigarString(){ return "*";} - java.util.List getGaps(); + default Cigar getCigar() { + return Cigar.fromCigarString(getCigarString()); + } + + List getGaps(); int getInferredInsertSize(); @@ -81,7 +89,7 @@ default int getLeadingHardClipLength() { int getMappingQuality(); - ReadMate getMate(); + default ReadMate getMate() { return null;} Strand getReadStrand(); @@ -107,7 +115,7 @@ default int getLeadingHardClipLength() { byte getPhred(double position); - Object getAttribute(String key); + default Object getAttribute(String key) { return null; } void setMateSequence(String sequence); @@ -125,15 +133,15 @@ default int getLeadingHardClipLength() { */ Color getYcColor(); - String getSample(); + default String getSample(){ return null;} - String getReadGroup(); + default String getReadGroup(){ return null;} - String getLibrary(); + default String getLibrary(){ return null;} String getClipboardString(double location, int mouseX); - void finish(); + default void finish(){}; default AlignmentBlock getInsertionAt(int position) { final AlignmentBlock[] insertions = getInsertions(); @@ -160,6 +168,13 @@ default Gap getDeletionAt(int position) { return null; } + /** + * Use the alignments CIGAR to count the clipping operations on either end + */ + default ClippingCounts getClippingCounts(){ + return ClippingCounts.fromCigar(getCigar()); + } + default void setHaplotypeName(String hap) {} default String getHaplotypeName() {return null;} @@ -189,4 +204,5 @@ default String getAlignmentValueString(double position, int mouseX, AlignmentTra default Alignment getSpecificAlignment(double location) { return this.contains(location) ? this : null; } + } diff --git a/src/main/java/org/broad/igv/sam/AlignmentDataManager.java b/src/main/java/org/broad/igv/sam/AlignmentDataManager.java index d638274529..d2c351eba2 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentDataManager.java +++ b/src/main/java/org/broad/igv/sam/AlignmentDataManager.java @@ -25,6 +25,7 @@ package org.broad.igv.sam; +import org.broad.igv.event.DataLoadedEvent; import org.broad.igv.logging.*; import org.broad.igv.Globals; import org.broad.igv.event.IGVEventBus; @@ -341,13 +342,13 @@ public void load(ReferenceFrame frame, intervalCache.add(loadedInterval); - packAlignments(renderOptions); + loadedInterval.packAlignments(renderOptions); } finally { currentlyLoading = null; } - // IGVEventBus.getInstance().post(new DataLoadedEvent(frame)); + IGVEventBus.getInstance().post(new DataLoadedEvent(frame)); } @@ -379,7 +380,7 @@ private boolean intervalInView(AlignmentInterval interval) { AlignmentInterval loadInterval(String chr, int start, int end, AlignmentTrack.RenderOptions renderOptions) { - String sequence = chrMappings.containsKey(chr) ? chrMappings.get(chr) : chr; + String sequence = chrMappings.getOrDefault(chr, chr); DownsampleOptions downsampleOptions = new DownsampleOptions(); @@ -390,7 +391,7 @@ AlignmentInterval loadInterval(String chr, int start, int end, AlignmentTrack.Re AlignmentTileLoader.AlignmentTile t = getLoader().loadTile(sequence, start, end, spliceJunctionHelper, downsampleOptions, peStats, bisulfiteContext, renderOptions); - List alignments = t.getAlignments(); + List alignments = t.getAlignments(); List downsampledIntervals = t.getDownsampledIntervals(); return new AlignmentInterval(chr, start, end, alignments, t.getCounts(), spliceJunctionHelper, downsampledIntervals); } diff --git a/src/main/java/org/broad/igv/sam/AlignmentInterval.java b/src/main/java/org/broad/igv/sam/AlignmentInterval.java index 5b567b27de..72f0a358a0 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentInterval.java +++ b/src/main/java/org/broad/igv/sam/AlignmentInterval.java @@ -102,7 +102,7 @@ static Alignment getFeatureContaining(List features, int right) { * @param option * @param location */ - public void sortRows(SortOption option, double location, String tag, boolean invertSort) { + public void sortRows(SortOption option, double location, String tag, boolean invertSort, Set priorityRecords) { PackedAlignments packedAlignments = getPackedAlignments(); if (packedAlignments == null) { @@ -111,7 +111,14 @@ public void sortRows(SortOption option, double location, String tag, boolean inv final int center = (int) location; byte referenceBase = this.getReference(center); - final Comparator rowComparator = option.getComparator(center, referenceBase, tag, invertSort); + Comparator rowComparator = option.getComparator(center, referenceBase, tag, invertSort); + + if( priorityRecords != null && !priorityRecords.isEmpty()){ + rowComparator = Comparator.comparing((Row row) -> row.getAlignments().stream() + .anyMatch( aln -> priorityRecords.contains(aln.getReadName()))) + .reversed() + .thenComparing(rowComparator); + } for (List alignmentRows : packedAlignments.values()) { alignmentRows.sort(rowComparator); diff --git a/src/main/java/org/broad/igv/sam/AlignmentPacker.java b/src/main/java/org/broad/igv/sam/AlignmentPacker.java index f8577792e8..009ca3b854 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentPacker.java +++ b/src/main/java/org/broad/igv/sam/AlignmentPacker.java @@ -29,6 +29,7 @@ */ package org.broad.igv.sam; +import htsjdk.samtools.SAMTag; import org.broad.igv.logging.*; import org.broad.igv.feature.Range; import org.broad.igv.feature.Strand; @@ -81,19 +82,13 @@ public PackedAlignments packAlignments( } else { // Separate alignments into groups. - Map> groupedAlignments = new HashMap>(); - Iterator iter = alList.iterator(); - while (iter.hasNext()) { - Alignment alignment = iter.next(); + Map> groupedAlignments = new HashMap<>(); + for (final Alignment alignment : alList) { Object groupKey = getGroupValue(alignment, renderOptions); if (groupKey == null) { groupKey = NULL_GROUP_VALUE; } - List groupList = groupedAlignments.get(groupKey); - if (groupList == null) { - groupList = new ArrayList<>(1000); - groupedAlignments.put(groupKey, groupList); - } + List groupList = groupedAlignments.computeIfAbsent(groupKey, k -> new ArrayList<>(1000)); groupList.add(alignment); } @@ -104,7 +99,7 @@ public PackedAlignments packAlignments( if(renderOptions.isInvertGroupSorting()){ groupComparator = groupComparator.reversed(); } - Collections.sort(keys, groupComparator); + keys.sort(groupComparator); for (Object key : keys) { List alignmentRows = new ArrayList<>(10000); @@ -125,7 +120,6 @@ private void pack(List alList, AlignmentTrack.RenderOptions renderOpt Map pairs = null; boolean isPairedAlignments = renderOptions.isViewPairs(); - String linkByTag = renderOptions.getLinkByTag(); if (isPairedAlignments) { pairs = new HashMap<>(1000); @@ -293,7 +287,7 @@ private List linkByTag(List alList, String tag) { } } - // Now copy list, de-linking orhpaned alignments (alignments with no linked mates) + // Now copy list, de-linking orphaned alignments (alignments with no linked mates) List delinkedList = new ArrayList<>(alList.size()); for (Alignment a : bcList) { if (a instanceof LinkedAlignment) { @@ -436,6 +430,8 @@ private Object getGroupValue(Alignment al, AlignmentTrack.RenderOptions renderOp } else { return mate.getChr(); } + case CHIMERIC: + return al.getAttribute(SAMTag.SA.name()) != null ? "CHIMERIC" : ""; case SUPPLEMENTARY: return al.isSupplementary() ? "SUPPLEMENTARY" : ""; case REFERENCE_CONCORDANCE: @@ -670,7 +666,7 @@ public int getBucketCount() { } } - private class PairOrientationComparator implements Comparator { + private static class PairOrientationComparator implements Comparator { private final List orientationTypes; //private final Set orientationNames = new HashSet(AlignmentTrack.OrientationType.values().length); diff --git a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java index aba8df86cc..a062b45987 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java +++ b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java @@ -43,22 +43,41 @@ import org.broad.igv.track.RenderContext; import org.broad.igv.track.Track; import org.broad.igv.ui.FontManager; -import org.broad.igv.ui.color.*; +import org.broad.igv.ui.color.ColorPalette; +import org.broad.igv.ui.color.ColorTable; +import org.broad.igv.ui.color.ColorUtilities; +import org.broad.igv.ui.color.GreyscaleColorTable; +import org.broad.igv.ui.color.HSLColorTable; +import org.broad.igv.ui.color.PaletteColorTable; import org.broad.igv.util.ChromosomeColors; import java.awt.*; import java.awt.geom.Rectangle2D; -import java.util.*; +import java.util.HashMap; import java.util.List; - -import static org.broad.igv.prefs.Constants.*; +import java.util.Map; + +import static org.broad.igv.prefs.Constants.SAM_ALLELE_THRESHOLD; +import static org.broad.igv.prefs.Constants.SAM_BASE_QUALITY_MAX; +import static org.broad.igv.prefs.Constants.SAM_BASE_QUALITY_MIN; +import static org.broad.igv.prefs.Constants.SAM_CLIPPING_THRESHOLD; +import static org.broad.igv.prefs.Constants.SAM_COLOR_A; +import static org.broad.igv.prefs.Constants.SAM_COLOR_C; +import static org.broad.igv.prefs.Constants.SAM_COLOR_G; +import static org.broad.igv.prefs.Constants.SAM_COLOR_N; +import static org.broad.igv.prefs.Constants.SAM_COLOR_T; +import static org.broad.igv.prefs.Constants.SAM_FLAG_CLIPPING; +import static org.broad.igv.prefs.Constants.SAM_FLAG_LARGE_INDELS; +import static org.broad.igv.prefs.Constants.SAM_LARGE_INDELS_THRESHOLD; +import static org.broad.igv.prefs.Constants.SAM_SHOW_CENTER_LINE; +import static org.broad.igv.prefs.Constants.SAM_SHOW_CONNECTED_CHR_NAME; /** * @author jrobinso */ public class AlignmentRenderer { - private static Logger log = LogManager.getLogger(AlignmentRenderer.class); + private static final Logger log = LogManager.getLogger(AlignmentRenderer.class); private static final Color negStrandColor = new Color(150, 150, 230); @@ -72,18 +91,22 @@ public class AlignmentRenderer { private static final Color RL_COLOR = new Color(0, 150, 0); private static final Color RR_COLOR = new Color(20, 50, 200); private static final Color LL_COLOR = new Color(0, 150, 150); - private static Color smallISizeColor = new Color(0, 0, 150); - private static Color largeISizeColor = new Color(200, 0, 0); + private static final Color smallISizeColor = new Color(0, 0, 150); + private static final Color largeISizeColor = new Color(200, 0, 0); private static final Color OUTLINE_COLOR = new Color(185, 185, 185); + //chimeric read connecting on the same contig colors + private static final Color INVERSION_COLOR = new Color( 200, 0, 0); + private static final Color NON_INVERSION_COLOR = new Color ( 230, 150, 150); + // Clipping colors - private static Color clippedColor = new Color(255, 20, 147); + private static final Color clippedColor = new Color(255, 20, 147); // Indel colors public static Color purple = new Color(118, 24, 220); - private static Color deletionColor = Color.black; - private static Color skippedColor = new Color(150, 184, 200); - private static Color unknownGapColor = new Color(0, 150, 0); + private static final Color deletionColor = Color.black; + private static final Color skippedColor = new Color(150, 184, 200); + private static final Color unknownGapColor = new Color(0, 150, 0); // Bisulfite colors private static final Color bisulfiteColorFw1 = new Color(195, 195, 195); @@ -102,7 +125,7 @@ public class AlignmentRenderer { public static final Color GROUP_DIVIDER_COLOR = new Color(200, 200, 200); // A "dummy" reference for soft-clipped reads. - private static byte[] softClippedReference = new byte[1000]; + private static final byte[] softClippedReference = new byte[1000]; private static ColorTable readGroupColors; private static ColorTable sampleColors; @@ -116,7 +139,7 @@ private static void initializeTagTypes() { // pre-seed from orientation colors // fr Orientations (e.g. Illumina paired-end libraries) - frOrientationTypes = new HashMap(); + frOrientationTypes = new HashMap<>(); //LR frOrientationTypes.put("F1R2", AlignmentTrack.OrientationType.LR); frOrientationTypes.put("F2R1", AlignmentTrack.OrientationType.LR); @@ -139,7 +162,7 @@ private static void initializeTagTypes() { frOrientationTypes.put("RF", AlignmentTrack.OrientationType.RL); // rf orienation (e.g. Illumina mate-pair libraries) - rfOrientationTypes = new HashMap(); + rfOrientationTypes = new HashMap<>(); //LR rfOrientationTypes.put("R1F2", AlignmentTrack.OrientationType.LR); rfOrientationTypes.put("R2F1", AlignmentTrack.OrientationType.LR); @@ -162,7 +185,7 @@ private static void initializeTagTypes() { rfOrientationTypes.put("FR", AlignmentTrack.OrientationType.RL); // f1f2 orienation (e.g. SOLID libraries, AlignmentTrack.OrientationType.second read appears first on + strand (leftmost)) - f2f1OrientationTypes = new HashMap(); + f2f1OrientationTypes = new HashMap<>(); //LR f2f1OrientationTypes.put("F2F1", AlignmentTrack.OrientationType.LR); f2f1OrientationTypes.put("R1R2", AlignmentTrack.OrientationType.LR); @@ -179,8 +202,8 @@ private static void initializeTagTypes() { f2f1OrientationTypes.put("R2R1", AlignmentTrack.OrientationType.RL); f2f1OrientationTypes.put("F1F2", AlignmentTrack.OrientationType.RL); - // f1f2 orienation (e.g. SOLID libraries, AlignmentTrack.OrientationType.actually is this one even possible?) - f1f2OrientationTypes = new HashMap(); + // f1f2 orientation (e.g. SOLID libraries, AlignmentTrack.OrientationType.actually is this one even possible?) + f1f2OrientationTypes = new HashMap<>(); //LR f1f2OrientationTypes.put("F1F2", AlignmentTrack.OrientationType.LR); f1f2OrientationTypes.put("R2R1", AlignmentTrack.OrientationType.LR); @@ -202,7 +225,7 @@ private static void initializeTagColors() { movieColors = new PaletteColorTable(palette); zmwColors = new PaletteColorTable(palette); defaultTagColors = new PaletteColorTable(palette); - tagValueColors = new HashMap(); + tagValueColors = new HashMap<>(); typeToColorMap = new HashMap<>(5); typeToColorMap.put(AlignmentTrack.OrientationType.LL, LL_COLOR); @@ -214,7 +237,7 @@ private static void setNucleotideColors() { IGVPreferences prefs = PreferencesManager.getPreferences(); - nucleotideColors = new HashMap(); + nucleotideColors = new HashMap<>(); Color a = ColorUtilities.stringToColor(prefs.get(SAM_COLOR_A), Color.green); Color c = ColorUtilities.stringToColor(prefs.get(SAM_COLOR_C), Color.blue); @@ -262,7 +285,6 @@ private void initializeGraphics(RenderContext context) { Graphics2D g2 = context.getGraphics2D("LINK_LINE"); alpha = 0.3f; - type = AlphaComposite.SRC_OVER; alignmentAlphaComposite = AlphaComposite.getInstance(type, alpha); g2.setComposite(alignmentAlphaComposite); @@ -373,7 +395,7 @@ private void drawLinkedAlignment(LinkedAlignment alignment, Rectangle rowRect, R List barcodedAlignments = alignment.alignments; if (barcodedAlignments.size() > 0) { - boolean mixedStrand = (alignment instanceof LinkedAlignment && alignment.getStrand() == Strand.NONE); + boolean mixedStrand = alignment.getStrand() == Strand.NONE; Alignment firstAlignment = barcodedAlignments.get(0); if (barcodedAlignments.size() > 1) { Graphics2D gline = context.getGraphics2D("LINK_LINE"); @@ -576,15 +598,15 @@ private void drawAlignment( /* Clipping */ boolean flagClipping = prefs.getAsBoolean(SAM_FLAG_CLIPPING); int clippingThreshold = prefs.getAsInt(SAM_CLIPPING_THRESHOLD); - int[] clipping = SAMAlignment.getClipping(alignment.getCigarString()); - boolean leftClipped = flagClipping && ((clipping[0] + clipping[1]) > clippingThreshold); - boolean rightClipped = flagClipping && ((clipping[2] + clipping[3]) > clippingThreshold); + ClippingCounts clipping = alignment.getClippingCounts(); + boolean leftClipped = flagClipping && (clipping.getLeft() > clippingThreshold); + boolean rightClipped = flagClipping && (clipping.getRight() > clippingThreshold); double bpStart = context.getOrigin(); double bpEnd = Math.ceil(context.getEndLocation()); // Draw gaps (deletions and junctions) - java.util.List gaps = alignment.getGaps(); + List gaps = alignment.getGaps(); if (gaps != null) { for (Gap gap : gaps) { int gapStart = gap.getStart(); @@ -643,8 +665,9 @@ private void drawAlignment( // Get a graphics context for outlining alignment blocks. Graphics2D outlineGraphics = null; final HashMap selectedReadNames = this.track.getSelectedReadNames(); - if (selectedReadNames.containsKey(alignment.getReadName())) { - Color c = selectedReadNames.get(alignment.getReadName()); + final String readName = alignment.getReadName(); + if (selectedReadNames.containsKey(readName)) { + Color c = selectedReadNames.get(readName); c = (c == null) ? Color.blue : c; outlineGraphics = context.getGraphics2D("THICK_STROKE"); gAlignment.setColor(c); @@ -711,14 +734,11 @@ private void drawAlignment( if (outlineGraphics != null) { outlineGraphics.draw(blockShape); } - if (leftmost && leftClipped) { - clippedGraphics.drawLine(xPoly[0], yPoly[0], xPoly[1], yPoly[1]); - clippedGraphics.drawLine(xPoly[5], yPoly[5] - 1, xPoly[0], yPoly[0]); - } - if (rightmost && rightClipped) { - clippedGraphics.drawLine(xPoly[2], yPoly[2], xPoly[3], yPoly[3]); - clippedGraphics.drawLine(xPoly[3], yPoly[3], xPoly[4], yPoly[4] - 1); - } + + final SupplementaryAlignment.SupplementaryNeighbors supplementaryRenderingInfo = SupplementaryAlignment.getAdjacentSupplementaryReads(alignment); + final boolean drawLeftClip = leftmost && leftClipped; + final boolean drawRightClip = rightmost && rightClipped; + drawClippedEnds(clippedGraphics, xPoly, yPoly, drawLeftClip, drawRightClip, supplementaryRenderingInfo); } leftmost = false; } @@ -892,6 +912,101 @@ private void drawAlignment( } + private static void drawClippedEnds(final Graphics2D g, final int[] xPoly, final int[] yPoly, + final boolean drawLeftClip, final boolean drawRightClip, + final SupplementaryAlignment.SupplementaryNeighbors sri) { + /* + 5 4 + 0 <|=======|> 3 + 1 2 + */ + final int xLeftPoint = xPoly[0]; + final int xLeft = xPoly[1]; + final int xRightPoint = xPoly[3]; + final int xRight = xPoly[2]; + final int yMiddle = yPoly[0]; + final int yBottom = yPoly[5]; + final int yTop = yPoly[1]; + final Color savedColor = g.getColor(); + final int arrowWidth = 3; + String leftContigLabel = null; + String rightContigLabel = null; + try { + //left side + if (drawLeftClip) { + if (sri != null && sri.previous != null) { + final SupplementaryAlignment previous = sri.previous; + if (previous.contigsMatch(sri.alignment)) { + g.setColor(previous.getStrand() == sri.alignment.getReadStrand() ? NON_INVERSION_COLOR : INVERSION_COLOR); + } else { + if (previous.getContig() != null) { + g.setColor(ChromosomeColors.getColor(previous.getContig())); + leftContigLabel = previous.getContig(); + } else { + log.warn("previous is missing contig: " + previous); + } + } + final Polygon thickLeftArrow = new Polygon( + new int[]{xLeftPoint, xLeft, xLeft + arrowWidth, xLeft + arrowWidth, xLeft}, + new int[]{yMiddle, yBottom, yBottom, yTop, yTop}, + 5); + g.drawPolygon(thickLeftArrow); + g.fillPolygon(thickLeftArrow); + g.setColor(savedColor); + } + g.drawLine(xLeftPoint, yMiddle, xLeft, yBottom); + g.drawLine(xLeft, yTop - 1, xLeftPoint, yMiddle); + } + //right side + if (drawRightClip) { + if (sri != null && sri.next != null) { + final SupplementaryAlignment next = sri.next; + if (next.contigsMatch(sri.alignment)) { + //TODO Set to scaled color by contig position + g.setColor(next.getStrand() == sri.alignment.getReadStrand() ? NON_INVERSION_COLOR : INVERSION_COLOR); + } else { + g.setColor(ChromosomeColors.getColor(next.getContig())); + rightContigLabel = next.getContig(); + } + Polygon thickRightArrow = new Polygon(new int[]{xRightPoint, xRight, xRight - arrowWidth, xRight - arrowWidth, xRight}, + new int[]{yMiddle, yBottom, yBottom, yTop, yTop}, 5); + g.drawPolygon(thickRightArrow); + g.fillPolygon(thickRightArrow); + g.setColor(savedColor); + } + g.drawLine(xRight, yBottom, xRightPoint, yMiddle); + g.drawLine(xRightPoint, yMiddle, xRight, yTop - 1); + } + //contig names + if(PreferencesManager.getPreferences().getAsBoolean(SAM_SHOW_CONNECTED_CHR_NAME)) { + if (leftContigLabel != null || rightContigLabel != null) { + final int height = yBottom - yTop; + int textHeight = g.getFontMetrics().getHeight(); + if (textHeight <= height + 4) { + int leftTextWidth = leftContigLabel != null ? g.getFontMetrics().stringWidth(leftContigLabel) : 0; + int rightContigWidth = rightContigLabel != null ? g.getFontMetrics().stringWidth(rightContigLabel) : 0; + + final int width = xRight - xLeft; + int totalTextWidth = leftTextWidth + rightContigWidth + g.getFontMetrics().stringWidth(" "); + final int distanceFromArrow = 4; + if (totalTextWidth <= width + 2 * distanceFromArrow) { + if (leftContigLabel != null) { + g.setColor(ChromosomeColors.getColor(leftContigLabel)); + g.drawString(leftContigLabel, xLeft + distanceFromArrow, yBottom - 1); + } + if (rightContigLabel != null) { + g.setColor(ChromosomeColors.getColor(rightContigLabel)); + g.drawString(rightContigLabel, xRight - (rightContigWidth + distanceFromArrow), yBottom - 1); + } + } + } + } + } + } finally { + g.setColor(savedColor); + } + } + /** * Draw the base using either a letter or character, using the given color, * depending on size and bisulfite status diff --git a/src/main/java/org/broad/igv/sam/AlignmentTileLoader.java b/src/main/java/org/broad/igv/sam/AlignmentTileLoader.java index 72f7833dea..3d30843d19 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTileLoader.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTileLoader.java @@ -26,6 +26,7 @@ package org.broad.igv.sam; import htsjdk.samtools.SAMFileHeader; +import htsjdk.samtools.SAMTag; import htsjdk.samtools.util.CloseableIterator; import org.broad.igv.logging.*; import org.broad.igv.Globals; @@ -60,7 +61,7 @@ public class AlignmentTileLoader implements IGVEventObserver { static DecimalFormat df = new DecimalFormat("###,###,###"); - private static Set> activeLoaders = Collections.synchronizedSet(new HashSet()); + private static Set> activeLoaders = Collections.synchronizedSet(new HashSet<>()); /** * Flag to mark a corrupt index. Without this attempted reads will continue in an infinite loop diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrack.java b/src/main/java/org/broad/igv/sam/AlignmentTrack.java index 3f306adf42..c8d6226df6 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrack.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrack.java @@ -28,6 +28,7 @@ import org.broad.igv.Globals; import org.broad.igv.event.AlignmentTrackEvent; +import org.broad.igv.event.DataLoadedEvent; import org.broad.igv.event.IGVEventBus; import org.broad.igv.event.IGVEventObserver; import org.broad.igv.feature.FeatureUtils; @@ -65,6 +66,7 @@ import java.awt.geom.Rectangle2D; import java.util.List; import java.util.*; +import java.util.function.Consumer; import static org.broad.igv.prefs.Constants.*; @@ -74,7 +76,7 @@ public class AlignmentTrack extends AbstractTrack implements IGVEventObserver { - private static Logger log = LogManager.getLogger(AlignmentTrack.class); + private static final Logger log = LogManager.getLogger(AlignmentTrack.class); // Alignment colors static final Color DEFAULT_ALIGNMENT_COLOR = new Color(185, 185, 185); //200, 200, 200); @@ -151,6 +153,7 @@ public enum GroupOption { PAIR_ORIENTATION("pair orientation"), MATE_CHROMOSOME("chromosome of mate"), NONE("none"), + CHIMERIC("chimeric"), SUPPLEMENTARY("supplementary flag"), BASE_AT_POS("base at position"), MOVIE("movie"), @@ -286,6 +289,7 @@ public static boolean isBisulfiteColorType(ColorOption o) { private ColorTable readNamePalette; private final HashMap selectedReadNames = new HashMap<>(); + private final HashMap> actionToPerformOnFrameLoad = new HashMap<>(); /** * Create a new alignment track @@ -335,7 +339,7 @@ public AlignmentTrack(ResourceLocator locator, AlignmentDataManager dataManager, spliceJunctionTrack.setHeight(60); this.spliceJunctionTrack = spliceJunctionTrack; - if (renderOptions.colorOption == ColorOption.BISULFITE) { + if (renderOptions.getColorOption() == ColorOption.BISULFITE) { setExperimentType(ExperimentType.BISULFITE); } readNamePalette = new PaletteColorTable(ColorUtilities.getDefaultPalette()); @@ -345,6 +349,7 @@ public AlignmentTrack(ResourceLocator locator, AlignmentDataManager dataManager, IGVEventBus.getInstance().subscribe(FrameManager.ChangeEvent.class, this); IGVEventBus.getInstance().subscribe(AlignmentTrackEvent.class, this); + IGVEventBus.getInstance().subscribe(DataLoadedEvent.class, this); } public void init() { @@ -383,6 +388,12 @@ public void receiveEvent(Object event) { break; } + } else if( event instanceof DataLoadedEvent){ + final DataLoadedEvent dataLoaded = (DataLoadedEvent) event; + actionToPerformOnFrameLoad.computeIfPresent(dataLoaded.getReferenceFrame(), (k, v) -> { + v.accept(k); + return null; + }); } } @@ -598,8 +609,8 @@ private void renderAlignments(RenderContext context, Rectangle inputRect) { } // Check for YC tag - if (renderOptions.colorOption == null && dataManager.hasYCTags()) { - renderOptions.colorOption = ColorOption.YC_TAG; + if (renderOptions.getColorOption() == null && dataManager.hasYCTags()) { + renderOptions.setColorOption(ColorOption.YC_TAG); } Map peStats = dataManager.getPEStats(); @@ -815,15 +826,20 @@ private InsertionInterval getInsertionInterval(ReferenceFrame frame, int x, int return null; } - public void sortRows(final SortOption option, final Double location, final String tag, final boolean invertSort) { + public void sortRows(final SortOption option, final Double location, final String tag, final boolean invertSort, final Set priorityRecords) { final List frames = FrameManager.getFrames(); for (ReferenceFrame frame : frames) { - final double actloc = location != null ? location : frame.getCenter(); - final AlignmentInterval interval = getDataManager().getLoadedInterval(frame); - if(interval != null) { - interval.sortRows(option, actloc, tag, invertSort); + Consumer sort = (ReferenceFrame f) -> { + final AlignmentInterval interval = getDataManager().getLoadedInterval(f); + final double actloc = location != null ? location : f.getCenter(); + interval.sortRows(option, actloc, tag, invertSort, priorityRecords); + }; + //If the data is loaded sort now, otherwise delay until we get a message that it is loaded. + if(getDataManager().isLoaded(frame)){ + sort.accept(frame); } else { - log.warn("Attempt to sort alignments prior to loading"); + log.debug("Attempt to sort alignments prior to loading"); + actionToPerformOnFrameLoad.put(frame, sort); } } } @@ -854,7 +870,6 @@ public void groupAlignments(GroupOption option, String tag, Range pos) { renderOptions.setGroupByOption(option); dataManager.packAlignments(renderOptions); repaint(); - } public void setBisulfiteContext(BisulfiteContext option) { @@ -1009,7 +1024,7 @@ private void clearCaches() { public void setViewAsPairs(boolean vAP) { // TODO -- generalize this test to all incompatible pairings - if (vAP && renderOptions.groupByOption == GroupOption.STRAND) { + if (vAP && renderOptions.getGroupByOption() == GroupOption.STRAND) { boolean ungroup = MessageUtils.confirm("\"View as pairs\" is incompatible with \"Group by strand\". Ungroup?"); if (ungroup) { renderOptions.setGroupByOption(null); @@ -1026,39 +1041,6 @@ public void setViewAsPairs(boolean vAP) { public enum ExperimentType {OTHER, RNA, BISULFITE, THIRD_GEN, UNKOWN} - class RenderRollback { - final ColorOption colorOption; - final GroupOption groupByOption; - final String groupByTag; - final String colorByTag; - final String linkByTag; - final DisplayMode displayMode; - final int expandedHeight; - final boolean showGroupLine; - - RenderRollback(RenderOptions renderOptions, DisplayMode displayMode) { - this.colorOption = renderOptions.colorOption; - this.groupByOption = renderOptions.groupByOption; - this.colorByTag = renderOptions.colorByTag; - this.groupByTag = renderOptions.groupByTag; - this.displayMode = displayMode; - this.expandedHeight = AlignmentTrack.this.expandedHeight; - this.showGroupLine = AlignmentTrack.this.showGroupLine; - this.linkByTag = renderOptions.linkByTag; - } - - void restore(RenderOptions renderOptions) { - renderOptions.colorOption = this.colorOption; - renderOptions.groupByOption = this.groupByOption; - renderOptions.colorByTag = this.colorByTag; - renderOptions.groupByTag = this.groupByTag; - renderOptions.linkByTag = this.linkByTag; - AlignmentTrack.this.expandedHeight = this.expandedHeight; - AlignmentTrack.this.showGroupLine = this.showGroupLine; - AlignmentTrack.this.setDisplayMode(this.displayMode); - } - } - public boolean isRemoved() { return removed; } @@ -1313,6 +1295,7 @@ public boolean includeStandardItems() { public static class RenderOptions implements Cloneable, Persistable { public static final String NAME = "RenderOptions"; + private static final Logger log = LogManager.getLogger(RenderOptions.class); private AlignmentTrack track; private Boolean shadeBasesOption; diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index 1dedbbac82..d672926a5f 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -1,5 +1,6 @@ package org.broad.igv.sam; +import com.google.gson.annotations.Since; import htsjdk.samtools.SAMTag; import htsjdk.samtools.util.Locatable; import org.broad.igv.Globals; @@ -17,6 +18,7 @@ import org.broad.igv.track.Track; import org.broad.igv.track.TrackClickEvent; import org.broad.igv.track.TrackMenuUtils; +import org.broad.igv.ui.AlignmentDiagramFrame; import org.broad.igv.ui.IGV; import org.broad.igv.ui.InsertSizeSettingsDialog; import org.broad.igv.ui.panel.FrameManager; @@ -37,6 +39,8 @@ import java.awt.event.MouseEvent; import java.util.*; import java.util.List; +import java.util.concurrent.CompletableFuture; +import java.util.concurrent.Future; import java.util.function.BiConsumer; import java.util.stream.Collectors; @@ -48,7 +52,7 @@ */ class AlignmentTrackMenu extends IGVPopupMenu { - private static Logger log = LogManager.getLogger(AlignmentTrackMenu.class); + private static final Logger log = LogManager.getLogger(AlignmentTrackMenu.class); private final AlignmentTrack alignmentTrack; private final AlignmentDataManager dataManager; @@ -138,7 +142,7 @@ class AlignmentTrackMenu extends IGVPopupMenu { // Select alignment items addSeparator(); - addSelectByNameItem(); + addSelectByNameItem(alignmentTrack, e); addClearSelectionsMenuItem(); // Copy items @@ -179,7 +183,7 @@ private void addShowChimericRegions(final AlignmentTrack alignmentTrack, final T try { List supplementaryAlignments = SupplementaryAlignment.parseFromSATag(saTag); alignmentTrack.setSelectedAlignment(clickedAlignment); - addNewLociToFrames(e.getFrame(), supplementaryAlignments); + addNewLociToFrames(e.getFrame(), supplementaryAlignments, alignmentTrack.getSelectedReadNames().keySet()); } catch (final Exception ex) { MessageUtils.showMessage("Failed to handle SA tag: " + saTag + " due to " + ex.getMessage()); item.setEnabled(false); @@ -191,6 +195,25 @@ private void addShowChimericRegions(final AlignmentTrack alignmentTrack, final T add(item); } + private void addShowDiagram(final TrackClickEvent e, final Alignment clickedAlignment){ + JMenuItem item = new JMenuItem("Supplementary Reads Diagram"); + if (clickedAlignment != null && clickedAlignment.getAttribute(SAMTag.SA.name()) != null) { + item.setEnabled(true); + item.addActionListener(aEvt -> { + try { + final AlignmentDiagramFrame frame = new AlignmentDiagramFrame(clickedAlignment, new Dimension(500, 100)); + frame.setVisible(true); + } catch (final Exception ex) { + MessageUtils.showMessage("Failed to handle SA tag: " + clickedAlignment.getAttribute(SAMTag.SA.name()) + " due to " + ex.getMessage()); + item.setEnabled(false); + } + }); + } else { + item.setEnabled(false); + } + add(item); + } + private void addHaplotype(TrackClickEvent e) { @@ -322,11 +345,12 @@ private JMenu getBisulfiteContextMenuItem(ButtonGroup group) { } - void addSelectByNameItem() { - // Change track height by attribute + void addSelectByNameItem(AlignmentTrack track, TrackClickEvent e) { JMenuItem item = new JMenuItem("Select by name..."); + final Alignment alignment = track.getAlignmentAt(e); + String alignmentName = alignment == null ? "" : alignment.getReadName(); item.addActionListener(aEvt -> { - String val = MessageUtils.showInputDialog("Enter read name: "); + String val = MessageUtils.showInputDialog("Enter read name: ", alignmentName); if (val != null && val.trim().length() > 0) { alignmentTrack.getSelectedReadNames().put(val, alignmentTrack.getReadNamePalette().get(val)); alignmentTrack.repaint(); @@ -375,7 +399,7 @@ void addGroupMenuItem(final TrackClickEvent te) {//ReferenceFrame frame) { AlignmentTrack.GroupOption[] groupOptions = { AlignmentTrack.GroupOption.NONE, AlignmentTrack.GroupOption.STRAND, AlignmentTrack.GroupOption.FIRST_OF_PAIR_STRAND, AlignmentTrack.GroupOption.SAMPLE, AlignmentTrack.GroupOption.LIBRARY, AlignmentTrack.GroupOption.READ_GROUP, AlignmentTrack.GroupOption.MATE_CHROMOSOME, - AlignmentTrack.GroupOption.PAIR_ORIENTATION, AlignmentTrack.GroupOption.SUPPLEMENTARY, AlignmentTrack.GroupOption.REFERENCE_CONCORDANCE, + AlignmentTrack.GroupOption.PAIR_ORIENTATION, AlignmentTrack.GroupOption.CHIMERIC, AlignmentTrack.GroupOption.SUPPLEMENTARY, AlignmentTrack.GroupOption.REFERENCE_CONCORDANCE, AlignmentTrack.GroupOption.MOVIE, AlignmentTrack.GroupOption.ZMW, AlignmentTrack.GroupOption.READ_ORDER, AlignmentTrack.GroupOption.LINKED, AlignmentTrack.GroupOption.PHASE, AlignmentTrack.GroupOption.MAPPING_QUALITY }; @@ -477,7 +501,8 @@ void addSortMenuItem() { mappings.put("read order", SortOption.READ_ORDER); mappings.put("read name", SortOption.READ_NAME); mappings.put("aligned read length", SortOption.ALIGNED_READ_LENGTH); -// mappings.put("supplementary flag", SortOption.SUPPLEMENTARY); + mappings.put("left clip", SortOption.LEFT_CLIP); + mappings.put("right clip", SortOption.RIGHT_CLIP); if (dataManager.isPairedEnd()) { mappings.put("insert size", SortOption.INSERT_SIZE); @@ -873,21 +898,21 @@ void addCopySequenceItems(final TrackClickEvent te) { /* Add a "Copy left clipped sequence" item if there is left clipping. */ int minimumBlatLength = BlatClient.MINIMUM_BLAT_LENGTH; - int[] clipping = SAMAlignment.getClipping(alignment.getCigarString()); - if (clipping[1] > 0) { - String lcSeq = getClippedSequence(alignment.getReadSequence(), alignment.getReadStrand(), 0, clipping[1]); + ClippingCounts clipping = alignment.getClippingCounts(); + if (clipping.getLeftSoft() > 0) { + String lcSeq = getClippedSequence(alignment.getReadSequence(), alignment.getReadStrand(), 0, clipping.getLeftSoft()); final JMenuItem lccItem = new JMenuItem("Copy left-clipped sequence"); add(lccItem); lccItem.addActionListener(aEvt -> StringUtils.copyTextToClipboard(lcSeq)); } /* Add a "Copy right clipped sequence" item if there is right clipping. */ - if (clipping[3] > 0) { + if (clipping.getRightHard() > 0) { int seqLength = seq.length(); String rcSeq = getClippedSequence( alignment.getReadSequence(), alignment.getReadStrand(), - seqLength - clipping[3], + seqLength - clipping.getRightHard(), seqLength); final JMenuItem rccItem = new JMenuItem("Copy right-clipped sequence"); @@ -930,11 +955,11 @@ void addBlatClippingItems(final TrackClickEvent te) { } int minimumBlatLength = BlatClient.MINIMUM_BLAT_LENGTH; - int[] clipping = SAMAlignment.getClipping(alignment.getCigarString()); + ClippingCounts clipping = alignment.getClippingCounts(); /* Add a "BLAT left clipped sequence" item if there is significant left clipping. */ - if (clipping[1] > minimumBlatLength) { - String lcSeq = getClippedSequence(alignment.getReadSequence(), alignment.getReadStrand(), 0, clipping[1]); + if (clipping.getLeftSoft() > minimumBlatLength) { + String lcSeq = getClippedSequence(alignment.getReadSequence(), alignment.getReadStrand(), 0, clipping.getLeftSoft()); final JMenuItem lcbItem = new JMenuItem("BLAT left-clipped sequence"); add(lcbItem); lcbItem.addActionListener(aEvt -> @@ -942,14 +967,14 @@ void addBlatClippingItems(final TrackClickEvent te) { ); } /* Add a "BLAT right clipped sequence" item if there is significant right clipping. */ - if (clipping[3] > minimumBlatLength) { + if (clipping.getRightSoft() > minimumBlatLength) { String seq = alignment.getReadSequence(); int seqLength = seq.length(); String rcSeq = getClippedSequence( alignment.getReadSequence(), alignment.getReadStrand(), - seqLength - clipping[3], + seqLength - clipping.getRightSoft(), seqLength); final JMenuItem rcbItem = new JMenuItem("BLAT right-clipped sequence"); @@ -1081,7 +1106,7 @@ void addThirdGenItems(Alignment clickedAlignment, final TrackClickEvent tce) { //Supplementary/chimeric items, only if the read has an SA tag addShowChimericRegions(alignmentTrack, tce, clickedAlignment); - + addShowDiagram(tce, clickedAlignment); final JMenuItem qcItem = new JCheckBoxMenuItem("Quick consensus mode"); qcItem.setSelected(renderOptions.isQuickConsensusMode()); @@ -1166,6 +1191,7 @@ private void gotoMate(final ReferenceFrame frame, Alignment alignment) { int newStart = (int) Math.max(0, (start + (alignment.getEnd() - alignment.getStart()) / 2 - range / 2)); int newEnd = newStart + (int) range; frame.jumpTo(chr, newStart, newEnd); + sortSelectedReadsToTheTop(alignmentTrack.getSelectedReadNames().keySet()); frame.recordHistory(); } else { MessageUtils.showMessage("Alignment does not have mate, or it is not mapped."); @@ -1183,14 +1209,14 @@ private void splitScreenMate(ReferenceFrame frame, Alignment alignment) { ReadMate mate = alignment.getMate(); if (mate != null && mate.isMapped()) { alignmentTrack.setSelectedAlignment(alignment); - addNewLociToFrames(frame, List.of(mate)); + addNewLociToFrames(frame, List.of(mate), alignmentTrack.getSelectedReadNames().keySet()); } else { MessageUtils.showMessage("Alignment does not have mate, or it is not mapped."); } } } - private static void addNewLociToFrames(final ReferenceFrame frame, final List toIncludeInSplit) { + private static void addNewLociToFrames(final ReferenceFrame frame, final List toIncludeInSplit, final Set selectedReadNames) { final List newLoci = toIncludeInSplit.stream() .map(locatable -> getLocusStringForAlignment(frame, locatable)) .collect(Collectors.toList()); @@ -1198,9 +1224,26 @@ private static void addNewLociToFrames(final ReferenceFrame frame, final List selectedReadNames) { + //copy this in case it changes out from under us + Set selectedReadNameCopy = new HashSet<>(selectedReadNames); + UIUtilities.invokeOnEventThread(() -> + IGV.getInstance().sortAlignmentTracks(SortOption.NONE, null, null, false, selectedReadNameCopy)); } private static List createLociList(final ReferenceFrame frame, final List lociToAdd) { diff --git a/src/main/java/org/broad/igv/sam/ClippingCounts.java b/src/main/java/org/broad/igv/sam/ClippingCounts.java new file mode 100644 index 0000000000..00a5034f0c --- /dev/null +++ b/src/main/java/org/broad/igv/sam/ClippingCounts.java @@ -0,0 +1,139 @@ +package org.broad.igv.sam; + +import htsjdk.samtools.Cigar; +import htsjdk.samtools.CigarElement; +import htsjdk.samtools.CigarOperator; + +import java.util.regex.Matcher; +import java.util.regex.Pattern; + +public final class ClippingCounts { + private final static ClippingCounts NO_CLIPPING = new ClippingCounts(0, 0, 0, 0); + public static final Pattern LCLIP_PATTERN = Pattern.compile("^(([0-9]+)H)?(([0-9]+)S)?"); + public static final Pattern RCLIP_PATTERN = Pattern.compile("(([0-9]+)S)?(([0-9]+)H)?$"); + private final int leftHard; + private final int leftSoft; + private final int rightHard; + private final int rightSoft; + + @Override + public boolean equals(final Object o) { + if (this == o) return true; + if (!(o instanceof ClippingCounts)) return false; + + final ClippingCounts that = (ClippingCounts) o; + + if (leftHard != that.leftHard) return false; + if (leftSoft != that.leftSoft) return false; + if (rightHard != that.rightHard) return false; + return rightSoft == that.rightSoft; + } + + @Override + public int hashCode() { + int result = leftHard; + result = 31 * result + leftSoft; + result = 31 * result + rightHard; + result = 31 * result + rightSoft; + return result; + } + + public ClippingCounts(final int leftHard, final int leftSoft, final int rightSoft, final int rightHard) { + this.leftHard = leftHard; + this.leftSoft = leftSoft; + this.rightHard = rightHard; + this.rightSoft = rightSoft; + } + + public static ClippingCounts fromCigar(Cigar cigar) { + if (cigar == null || cigar.isEmpty() || !cigar.isClipped()) { + return NO_CLIPPING; + } else { + final int numberOfElements = cigar.numCigarElements(); + int leftHard = 0, leftSoft = 0, rightSoft = 0, rightHard = 0; + int i = 0; + for (; i < 2 && i <= numberOfElements; i++) { + final CigarElement element = cigar.getCigarElement(i); + final CigarOperator operator = element.getOperator(); + if (operator == CigarOperator.H) { + leftHard = element.getLength(); + } else if (operator == CigarOperator.S) { + leftSoft = element.getLength(); + break; + } else { + break; + } + } + for (int j = numberOfElements - 1; j > (numberOfElements - 3) && (j > i) && (j > 0); j--) { + final CigarElement element = cigar.getCigarElement(j); + final CigarOperator operator = element.getOperator(); + if (operator == CigarOperator.H) { + rightHard = element.getLength(); + } else if (operator == CigarOperator.S) { + rightSoft = element.getLength(); + break; + } else { + break; + } + } + return new ClippingCounts(leftHard, leftSoft, rightSoft, rightHard); + } + } + + public static ClippingCounts fromCigarString(String cigarString) { + // Identify the number of hard and soft clipped bases. + Matcher lclipMatcher = LCLIP_PATTERN.matcher(cigarString); + Matcher rclipMatcher = RCLIP_PATTERN.matcher(cigarString); + int lclipHard = 0, lclipSoft = 0, rclipHard = 0, rclipSoft = 0; + if (lclipMatcher.find()) { + lclipHard = lclipMatcher.group(2) == null ? 0 : Integer.parseInt(lclipMatcher.group(2), 10); + lclipSoft = lclipMatcher.group(4) == null ? 0 : Integer.parseInt(lclipMatcher.group(4), 10); + } + if (rclipMatcher.find()) { + rclipHard = rclipMatcher.group(4) == null ? 0 : Integer.parseInt(rclipMatcher.group(4), 10); + rclipSoft = rclipMatcher.group(2) == null ? 0 : Integer.parseInt(rclipMatcher.group(2), 10); + } + return new ClippingCounts(lclipHard, lclipSoft, rclipSoft, rclipHard); + } + + public boolean isClipped() { + return leftHard + leftSoft + rightHard + rightSoft > 0; + } + + public int getLeftHard() { + return leftHard; + } + + public int getLeftSoft() { + return leftSoft; + } + + public int getRightHard() { + return rightHard; + } + + public int getRightSoft() { + return rightSoft; + } + + public boolean isLeftClipped() { + return getLeft() > 0; + } + + public int getLeft() { + return leftSoft + leftHard; + } + + public boolean isRightClipped() { + return getRight() > 0; + } + + public int getRight() { + return rightSoft + rightHard; + } + + @Override + public String toString() { + return "<" + leftHard + "H" + leftSoft + "S" + "|" + rightSoft + "S" + rightHard + "H" + ">"; + } +} diff --git a/src/main/java/org/broad/igv/sam/CoverageTrack.java b/src/main/java/org/broad/igv/sam/CoverageTrack.java index e271bd767f..5a0730d24f 100644 --- a/src/main/java/org/broad/igv/sam/CoverageTrack.java +++ b/src/main/java/org/broad/igv/sam/CoverageTrack.java @@ -29,7 +29,6 @@ */ package org.broad.igv.sam; -import htsjdk.samtools.util.SequenceUtil; import org.broad.igv.Globals; import org.broad.igv.data.CoverageDataSource; import org.broad.igv.feature.FeatureUtils; @@ -40,8 +39,6 @@ import org.broad.igv.prefs.IGVPreferences; import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.renderer.*; -import org.broad.igv.sam.mods.BaseModificationColors; -import org.broad.igv.sam.mods.BaseModificationCounts; import org.broad.igv.sam.mods.BaseModificationCoverageRenderer; import org.broad.igv.tdf.TDFDataSource; import org.broad.igv.tdf.TDFReader; diff --git a/src/main/java/org/broad/igv/sam/DotAlignedAlignment.java b/src/main/java/org/broad/igv/sam/DotAlignedAlignment.java index ae7090149d..786ef6325b 100644 --- a/src/main/java/org/broad/igv/sam/DotAlignedAlignment.java +++ b/src/main/java/org/broad/igv/sam/DotAlignedAlignment.java @@ -90,11 +90,6 @@ public Color getYcColor() { return null; } - - public String getChromosome() { - return chromosome; - } - public String getChr() { return chromosome; } @@ -120,10 +115,6 @@ public AlignmentBlock[] getInsertions() { return null; } - public String getCigarString() { - return "*"; - } - public int getInferredInsertSize() { return 0; } @@ -132,10 +123,6 @@ public int getMappingQuality() { return 255; } - public ReadMate getMate() { - return null; - } - public boolean isProperPair() { return true; } @@ -212,22 +199,6 @@ public byte getPhred(double position) { return 0; } - public String getSample() { - return null; - } - - public String getReadGroup() { - return null; - } - - public String getLibrary() { - return null; - } - - public Object getAttribute(String key) { - return null; //To change body of implemented methods use File | Settings | File Templates. - } - @Override public List getGaps() { return null; @@ -255,10 +226,6 @@ public Strand getReadStrand() { return isNegativeStrand() ? Strand.NEGATIVE : Strand.POSITIVE; } - @Override - public void finish() { - } - @Override public boolean isPrimary() { return true; diff --git a/src/main/java/org/broad/igv/sam/FeatureWrappedAlignment.java b/src/main/java/org/broad/igv/sam/FeatureWrappedAlignment.java index dd2ee73599..e0519622ec 100644 --- a/src/main/java/org/broad/igv/sam/FeatureWrappedAlignment.java +++ b/src/main/java/org/broad/igv/sam/FeatureWrappedAlignment.java @@ -107,10 +107,6 @@ public AlignmentBlock[] getInsertions() { return null; } - public String getCigarString() { - return "*"; - } - public int getInferredInsertSize() { return 0; } @@ -119,10 +115,6 @@ public int getMappingQuality() { return 255; } - public ReadMate getMate() { - return null; - } - public boolean isProperPair() { return true; } @@ -199,22 +191,6 @@ public byte getPhred(double position) { return 0; } - public String getSample() { - return null; - } - - public String getReadGroup() { - return null; - } - - public String getLibrary() { - return null; - } - - public Object getAttribute(String key) { - return null; //To change body of implemented methods use File | Settings | File Templates. - } - public void setMateSequence(String sequence) { //To change body of implemented methods use File | Settings | File Templates. } @@ -260,10 +236,6 @@ public Strand getReadStrand() { return isNegativeStrand() ? Strand.NEGATIVE : Strand.POSITIVE; } - @Override - public void finish() { - } - @Override public boolean isPrimary() { return true; diff --git a/src/main/java/org/broad/igv/sam/LinkedAlignment.java b/src/main/java/org/broad/igv/sam/LinkedAlignment.java index 4d7b97ae70..e90eb6dc50 100644 --- a/src/main/java/org/broad/igv/sam/LinkedAlignment.java +++ b/src/main/java/org/broad/igv/sam/LinkedAlignment.java @@ -246,12 +246,6 @@ public AlignmentBlock[] getInsertions() { return insertions; } - - @Override - public String getCigarString() { - return null; - } - @Override public List getGaps() { return null; @@ -262,11 +256,6 @@ public int getInferredInsertSize() { return 0; } - @Override - public ReadMate getMate() { - return null; - } - @Override public Strand getReadStrand() { return null; @@ -374,17 +363,6 @@ public String getSample() { return sample; } - @Override - public String getReadGroup() { - - return null; - } - - @Override - public String getLibrary() { - return null; - } - @Override public String getClipboardString(double location, int mouseX) { return null; diff --git a/src/main/java/org/broad/igv/sam/PairedAlignment.java b/src/main/java/org/broad/igv/sam/PairedAlignment.java index 087ef0fd9b..831d9e1bb2 100644 --- a/src/main/java/org/broad/igv/sam/PairedAlignment.java +++ b/src/main/java/org/broad/igv/sam/PairedAlignment.java @@ -216,14 +216,7 @@ public String getReadSequence() { return firstAlignment == null ? "" : firstAlignment.getReadSequence(); } - public String getCigarString() { - return null; //To change body of implemented methods use File | Settings | File Templates. - } - - public ReadMate getMate() { - return null; //To change body of implemented methods use File | Settings | File Templates. - } - + @Override public boolean isPaired() { return false; //Counter intuitive, but the pair does not have a mate } @@ -232,19 +225,6 @@ public boolean isNegativeStrand() { return false; //To change body of implemented methods use File | Settings | File Templates. } - - public String getSample() { - return null; //To change body of implemented methods use File | Settings | File Templates. - } - - public String getReadGroup() { - return null; //To change body of implemented methods use File | Settings | File Templates. - } - - public Object getAttribute(String key) { - return null; //To change body of implemented methods use File | Settings | File Templates. - } - public void setMateSequence(String sequence) { //To change body of implemented methods use File | Settings | File Templates. } diff --git a/src/main/java/org/broad/igv/sam/ReducedMemoryAlignment.java b/src/main/java/org/broad/igv/sam/ReducedMemoryAlignment.java index 27f1ed150e..90fe24cf65 100644 --- a/src/main/java/org/broad/igv/sam/ReducedMemoryAlignment.java +++ b/src/main/java/org/broad/igv/sam/ReducedMemoryAlignment.java @@ -170,10 +170,6 @@ public AlignmentBlock[] getInsertions() { return insertions; } - public String getCigarString() { - return "*"; - } - public int getInferredInsertSize() { return 0; } @@ -182,10 +178,6 @@ public int getMappingQuality() { return 255; } - public ReadMate getMate() { - return null; - } - public boolean isProperPair() { return true; } @@ -281,22 +273,6 @@ public byte getPhred(double position) { return 0; } - public String getSample() { - return null; - } - - public String getReadGroup() { - return null; - } - - public String getLibrary() { - return null; - } - - public Object getAttribute(String key) { - return null; //To change body of implemented methods use File | Settings | File Templates. - } - @Override public List getGaps() { return gaps; @@ -323,10 +299,6 @@ public Strand getReadStrand() { return isNegativeStrand() ? Strand.NEGATIVE : Strand.POSITIVE; } - @Override - public void finish() { - } - @Override public boolean isPrimary() { return true; diff --git a/src/main/java/org/broad/igv/sam/SAMAlignment.java b/src/main/java/org/broad/igv/sam/SAMAlignment.java index 432faaeb4d..4e8e6cd436 100644 --- a/src/main/java/org/broad/igv/sam/SAMAlignment.java +++ b/src/main/java/org/broad/igv/sam/SAMAlignment.java @@ -29,9 +29,11 @@ */ package org.broad.igv.sam; +import htsjdk.samtools.Cigar; import htsjdk.samtools.SAMFileHeader; import htsjdk.samtools.SAMReadGroupRecord; import htsjdk.samtools.SAMRecord; +import htsjdk.samtools.SAMTag; import org.broad.igv.logging.*; import org.broad.igv.Globals; import org.broad.igv.feature.Strand; @@ -42,12 +44,12 @@ import org.broad.igv.sam.mods.BaseModificationUtils; import org.broad.igv.sam.mods.BaseModificationSet; import org.broad.igv.sam.smrt.SMRTKinetics; -import org.broad.igv.sam.smrt.SMRTKineticsDecoder; import org.broad.igv.ui.color.ColorUtilities; import java.awt.*; import java.util.*; import java.util.List; +import java.util.function.Supplier; import java.util.regex.Matcher; import java.util.regex.Pattern; @@ -56,7 +58,7 @@ */ public class SAMAlignment implements Alignment { - private static Logger log = LogManager.getLogger(SAMAlignment.class); + private static final Logger log = LogManager.getLogger(SAMAlignment.class); public static final char DELETE_CHAR = '-'; public static final char SKIP_CHAR = '='; @@ -116,6 +118,23 @@ public class SAMAlignment implements Alignment { private List baseModificationSets; private SMRTKinetics smrtKinetics; + private enum CacheKey {CLIPPING_COUNTS, SA_GROUP}; + + /** + * Use this to cache an expensive to compute value on this record and retrieve it as necessary. + */ + @SuppressWarnings("unchecked") + private T getCachedOrCompute(CacheKey key, Supplier supplier){ + final Object value = record.getTransientAttribute(key); + if (value == null) { + final T newValue = supplier.get(); + record.setTransientAttribute(key, newValue); + return newValue; + } else { + return (T)value; + } + } + public SAMAlignment(SAMRecord record) { this.record = record; @@ -195,6 +214,10 @@ public Object getAttribute(String key) { } } + private Object getAttribute(SAMTag key) { + return key == null ? null : record.getAttribute(key); + } + public boolean isFirstOfPair() { return isPaired() && (flags & FIRST_OF_PAIR_FLAG) != 0; } @@ -255,6 +278,17 @@ public String getCigarString() { return record.getCigarString(); } + @Override + public Cigar getCigar() { + return record.getCigar(); + } + + @Override + public ClippingCounts getClippingCounts(){ + return getCachedOrCompute(CacheKey.CLIPPING_COUNTS, + () -> ClippingCounts.fromCigar(record.getCigar())); + } + public String getReadSequence() { return record.getReadString(); } @@ -476,7 +510,7 @@ private void createAlignmentBlocks() { gapTypes = new char[nGaps]; } if (nRealGaps > 0) { - gaps = new ArrayList(); + gaps = new ArrayList<>(); } // Adjust start to include soft clipped bases a @@ -580,7 +614,7 @@ private void createAlignmentBlocks() { */ private static List buildOperators(String cigarString) { - java.util.List operators = new ArrayList(); + java.util.List operators = new ArrayList<>(); StringBuilder buffer = new StringBuilder(4); // Create list of cigar operators @@ -608,6 +642,7 @@ private static List buildOperators(String cigarString) { } + @Override public String getClipboardString(double location, int mouseX) { return getAlignmentValueString(location, mouseX, (AlignmentTrack.RenderOptions) null); } @@ -621,6 +656,7 @@ private Integer positionToReadIndex(double position) { return null; } + @Override public String getAlignmentValueString(double position, int mouseX, AlignmentTrack.RenderOptions renderOptions) { boolean truncate = renderOptions != null; @@ -754,37 +790,27 @@ public String getAlignmentValueString(double position, int mouseX, AlignmentTrac buf.append("Clipping = "); // Identify the number of hard and soft clipped bases. - Matcher lclipMatcher = Pattern.compile("^(([0-9]+)H)?(([0-9]+)S)?").matcher(cigarString); - Matcher rclipMatcher = Pattern.compile("(([0-9]+)S)?(([0-9]+)H)?$").matcher(cigarString); - int lclipHard = 0, lclipSoft = 0, rclipHard = 0, rclipSoft = 0; - if (lclipMatcher.find()) { - lclipHard = lclipMatcher.group(2) == null ? 0 : Integer.parseInt(lclipMatcher.group(2), 10); - lclipSoft = lclipMatcher.group(4) == null ? 0 : Integer.parseInt(lclipMatcher.group(4), 10); - } - if (rclipMatcher.find()) { - rclipHard = rclipMatcher.group(4) == null ? 0 : Integer.parseInt(rclipMatcher.group(4), 10); - rclipSoft = rclipMatcher.group(2) == null ? 0 : Integer.parseInt(rclipMatcher.group(2), 10); - } + ClippingCounts clipping = getClippingCounts(); - if (lclipHard + lclipSoft + rclipHard + rclipSoft == 0) { + if (!clipping.isClipped()){ buf.append("None"); } else { - if (lclipHard + lclipSoft > 0) { + if (clipping.isLeftClipped()) { buf.append("Left"); - if (lclipHard > 0) { - buf.append(" " + Globals.DECIMAL_FORMAT.format(lclipHard) + " hard"); + if (clipping.getLeftHard() > 0) { + buf.append(" " + Globals.DECIMAL_FORMAT.format(clipping.getLeftHard()) + " hard"); } - if (lclipSoft > 0) { - buf.append(" " + Globals.DECIMAL_FORMAT.format(lclipSoft) + " soft"); + if (clipping.getLeftSoft() > 0) { + buf.append(" " + Globals.DECIMAL_FORMAT.format(clipping.getLeftSoft()) + " soft"); } } - if (rclipHard + rclipSoft > 0) { - buf.append((lclipHard + lclipSoft > 0 ? "; " : "") + "Right"); - if (rclipHard > 0) { - buf.append(" " + Globals.DECIMAL_FORMAT.format(rclipHard) + " hard"); + if (clipping.isRightClipped()) { + buf.append((clipping.isLeftClipped() ? "; " : "") + "Right"); + if (clipping.getRightHard() > 0) { + buf.append(" " + Globals.DECIMAL_FORMAT.format(clipping.getRightHard()) + " hard"); } - if (rclipSoft > 0) { - buf.append(" " + Globals.DECIMAL_FORMAT.format(rclipSoft) + " soft"); + if (clipping.getRightSoft() > 0) { + buf.append(" " + Globals.DECIMAL_FORMAT.format(clipping.getRightSoft()) + " soft"); } } } @@ -814,10 +840,10 @@ public String getAlignmentValueString(double position, int mouseX, AlignmentTrac } } - Object suppAlignment = this.getAttribute("SA"); + Object suppAlignment = this.getAttribute(SAMTag.SA); if (suppAlignment != null) { buf.append("----------------------
"); - buf.append(getSupplAlignmentString(suppAlignment.toString())); + buf.append(getSupplAlignmentString()); buf.append("
"); } @@ -936,28 +962,43 @@ private String getAttributeString(boolean truncate) { private String getMlTagString(SAMRecord.SAMTagAndValue tag) { byte[] bytes = (byte[]) tag.value; - String buf = ""; + StringBuilder buf = new StringBuilder(); for (int i = 0; i < bytes.length; i++) { - if (i > 0) buf += ","; - buf += Byte.toUnsignedInt(bytes[i]); + if (i > 0) buf.append(","); + buf.append(Byte.toUnsignedInt(bytes[i])); } - return buf; + return buf.toString(); } - private String getSupplAlignmentString(String sa) { - - StringBuffer buf = new StringBuffer(); - buf.append("SupplementaryAlignments"); - String[] records = Globals.semicolonPattern.split(sa); - for (String rec : records) { + private String getSupplAlignmentString() { + StringBuilder sb = new StringBuilder(); + sb.append("SupplementaryAlignments"); + final List supplementaryAlignments = getSupplementaryAlignments(); + final int insertionIndex = SupplementaryAlignment.getInsertionIndex(this, supplementaryAlignments); + int i = 0; + for (SupplementaryAlignment sa: supplementaryAlignments) { + if(i == insertionIndex) { //Add this read into the list + sb.append(getThisReadDescriptionForSAList()); + } + i++; try { - SupplementaryAlignment a = new SupplementaryAlignment(rec); - buf.append("
" + a.printString()); + sb.append("
" + sa.printString()); } catch (Exception e) { - buf.append("
* Invalid SA entry (not listed) *"); + sb.append("
* Invalid SA entry (not listed) *"); } } - return buf.toString(); + if(i == insertionIndex){ + sb.append(getThisReadDescriptionForSAList()); + } + return sb.toString(); + } + + private String getThisReadDescriptionForSAList() { + return "
" + + "" + + chr + ":" + Globals.DECIMAL_FORMAT.format(getAlignmentStart() + 1) + "-" + Globals.DECIMAL_FORMAT.format(getAlignmentEnd()) + + " (" + getReadStrand().toShortString() + ")" + + ""; } @@ -994,12 +1035,6 @@ public java.util.List getGaps() { return gaps; } - - @Override - public void finish() { - - } - @Override public AlignmentBlock getInsertionAt(int position) { for (AlignmentBlock block : insertions) { @@ -1122,18 +1157,18 @@ public CigarOperator(int nBases, char operator) { public String getSynopsisString() { char st = isNegativeStrand() ? '-' : '+'; - Object nm = getAttribute("NM"); + Object nm = getAttribute(SAMTag.NM); String numMismatches = nm == null ? "?" : nm.toString(); int lenOnRef = getAlignmentEnd() - getAlignmentStart(); - int[] clipping = getClipping(getCigarString()); + ClippingCounts clipping = getClippingCounts(); String clippingString = ""; - if (clipping[0] + clipping[1] + clipping[2] + clipping[3] > 0) { - if (clipping[0] > 0) clippingString += clipping[0] + "H"; - if (clipping[1] > 0) clippingString += clipping[1] + "S"; + if (clipping.isClipped()) { + if (clipping.getLeftHard() > 0) clippingString += clipping.getLeftHard() + "H"; + if (clipping.getLeftSoft() > 0) clippingString += clipping.getLeftSoft() + "S"; clippingString += " ... "; - if (clipping[3] > 0) clippingString += clipping[3] + "S"; - if (clipping[2] > 0) clippingString += clipping[2] + "H"; + if (clipping.getRightSoft() > 0) clippingString += clipping.getRightSoft() + "S"; + if (clipping.getRightHard() > 0) clippingString += clipping.getRightHard() + "H"; } return chr + ":" + Globals.DECIMAL_FORMAT.format(getAlignmentStart()) + "-" + @@ -1143,22 +1178,6 @@ public String getSynopsisString() { } - public static int[] getClipping(String cigarString) { - // Identify the number of hard and soft clipped bases. - Matcher lclipMatcher = Pattern.compile("^(([0-9]+)H)?(([0-9]+)S)?").matcher(cigarString); - Matcher rclipMatcher = Pattern.compile("(([0-9]+)S)?(([0-9]+)H)?$").matcher(cigarString); - int lclipHard = 0, lclipSoft = 0, rclipHard = 0, rclipSoft = 0; - if (lclipMatcher.find()) { - lclipHard = lclipMatcher.group(2) == null ? 0 : Integer.parseInt(lclipMatcher.group(2), 10); - lclipSoft = lclipMatcher.group(4) == null ? 0 : Integer.parseInt(lclipMatcher.group(4), 10); - } - if (rclipMatcher.find()) { - rclipHard = rclipMatcher.group(4) == null ? 0 : Integer.parseInt(rclipMatcher.group(4), 10); - rclipSoft = rclipMatcher.group(2) == null ? 0 : Integer.parseInt(rclipMatcher.group(2), 10); - } - return new int[]{lclipHard, lclipSoft, rclipHard, rclipSoft}; - } - private static boolean operatorIsMatch(boolean showSoftClipped, char operator) { return operator == MATCH || operator == PERFECT_MATCH || operator == MISMATCH || (showSoftClipped && operator == SOFT_CLIP); @@ -1191,4 +1210,16 @@ public int getHapDistance() { return hapDistance; } + public List getSupplementaryAlignments(){ + return getCachedOrCompute(CacheKey.SA_GROUP, + () -> { + Object rawSAValue = this.getAttribute(SAMTag.SA); + if (rawSAValue == null) { + return null; + } else { + final List sas = Collections.unmodifiableList(SupplementaryAlignment.parseFromSATag(rawSAValue.toString())); + return sas; + } + }); + } } diff --git a/src/main/java/org/broad/igv/sam/SortOption.java b/src/main/java/org/broad/igv/sam/SortOption.java index 36f5a76d30..93e26787c9 100644 --- a/src/main/java/org/broad/igv/sam/SortOption.java +++ b/src/main/java/org/broad/igv/sam/SortOption.java @@ -1,11 +1,18 @@ package org.broad.igv.sam; +import htsjdk.samtools.SAMTag; +import htsjdk.samtools.util.Locatable; +import org.broad.igv.feature.genome.ChromosomeNameComparator; + import java.util.Comparator; +import java.util.List; import java.util.Objects; +import java.util.Set; import java.util.function.Function; import java.util.function.ToIntFunction; public enum SortOption { + START { @Override Comparator getAlignmentComparator(final int center, final String tag, final byte referenceBase) { @@ -100,6 +107,20 @@ Comparator getAlignmentComparator(final int center, final String tag, //todo It would be nice to sort by something smarter than hash code but the possibility of mixed tag types makes that more complicated } }, + LEFT_CLIP { + @Override + Comparator getAlignmentComparator(final int center, final String tag, final byte referenceBase) { + return Comparator.comparing((Alignment a) -> a.getClippingCounts().isLeftClipped()).reversed() + .thenComparing(Alignment::getAlignmentStart); + } + }, + RIGHT_CLIP { + @Override + Comparator getAlignmentComparator(final int center, final String tag, final byte referenceBase) { + return Comparator.comparing((Alignment a) -> a.getClippingCounts().isRightClipped()).reversed() + .thenComparing(Alignment::getAlignmentEnd); + } + }, SUPPLEMENTARY { @Override Comparator getAlignmentComparator(final int center, final String tag, final byte referenceBase) { @@ -154,4 +175,8 @@ public Comparator getComparator(final int center, final byte reference, fin // center alignment anyway abstract Comparator getAlignmentComparator(final int center, final String tag, final byte referenceBase); + + public static final Comparator POSITION_COMPARATOR = Comparator.nullsFirst(Comparator.comparing(Locatable::getContig, ChromosomeNameComparator.get())) + .thenComparing(Locatable::getStart) + .thenComparing(Locatable::getEnd); } diff --git a/src/main/java/org/broad/igv/sam/SupplementaryAlignment.java b/src/main/java/org/broad/igv/sam/SupplementaryAlignment.java index bb5cf87a67..8991b3e89d 100644 --- a/src/main/java/org/broad/igv/sam/SupplementaryAlignment.java +++ b/src/main/java/org/broad/igv/sam/SupplementaryAlignment.java @@ -1,69 +1,162 @@ package org.broad.igv.sam; +import htsjdk.samtools.Cigar; +import htsjdk.samtools.SAMTag; +import htsjdk.samtools.TextCigarCodec; import htsjdk.samtools.util.Locatable; import org.broad.igv.Globals; +import org.broad.igv.feature.Strand; +import org.broad.igv.feature.genome.Genome; +import org.broad.igv.feature.genome.GenomeManager; +import org.broad.igv.logging.LogManager; +import org.broad.igv.logging.Logger; +import java.util.ArrayList; import java.util.Arrays; +import java.util.Collections; +import java.util.Comparator; +import java.util.Iterator; import java.util.List; +import java.util.TreeSet; import java.util.stream.Collectors; +import java.util.stream.Stream; public class SupplementaryAlignment implements Locatable { + private static final Logger log = LogManager.getLogger(SupplementaryAlignment.class); - public String chr; - public int start; - public char strand; - public int mapQ; - public int numMismatches; - public int lenOnRef; - - - public SupplementaryAlignment(String rec) { - String[] tokens = Globals.commaPattern.split(rec); - chr = tokens[0]; - start = Integer.parseInt(tokens[1]); - strand = tokens[2].charAt(0); - mapQ = Integer.parseInt(tokens[4]); - numMismatches = Integer.parseInt(tokens[5]); - lenOnRef = computeLengthOnReference(tokens[3]); + public final String chr; + public final int start; + + public Strand getStrand() { + return strand; + } + + private final Strand strand; + private final Cigar cigar; + + public final int mapQ; + public final int numMismatches; + + //potentially expensive with very long reads, compute it lazily + private Integer lenOnRef = null; + + public SupplementaryAlignment(String chr, int start, Strand strand, Cigar cigar, int mapQ, int numMismatches){ + this.chr = chr; + this.start = start; + this.strand = strand; + this.cigar = cigar; + this.mapQ = mapQ; + this.numMismatches = numMismatches; + } + + public static int getInsertionIndex(final Alignment alignment, final List supplementaryAlignments) { + final SupplementaryAlignment alignmentAsSupplementary = new SupplementaryAlignment(null, 0, alignment.getReadStrand(), alignment.getCigar(), + 0, 0); //We're just using this as a placeholder for sorting since Alignment + // and SupplementaryAlignment don't share any common class + int insertionIndex = Collections.binarySearch(supplementaryAlignments, alignmentAsSupplementary, LEADING_CLIP_COMPARATOR); + if(insertionIndex > 0) { + log.warn("Saw 2 SupplementaryReads with the same leading clip length."); + } else { + insertionIndex = -1* (insertionIndex + 1); + } + return insertionIndex; + } + + public static SupplementaryNeighbors getAdjacentSupplementaryReads(final Alignment alignment) { + final List supplementaryAlignments; + + final SupplementaryNeighbors supplementaryRenderingInfo; + + if( alignment instanceof SAMAlignment){ + supplementaryAlignments = ((SAMAlignment) alignment).getSupplementaryAlignments(); + } else { + final Object rawSATag = alignment.getAttribute(SAMTag.SA.name()); + supplementaryAlignments = rawSATag == null ? null : new ArrayList<>(parseFromSATag(rawSATag.toString())); + } + + + if(supplementaryAlignments != null && !supplementaryAlignments.isEmpty()) { + + int insertionIndex = getInsertionIndex(alignment, supplementaryAlignments); + if( insertionIndex < 0 ){ + log.warn("Encountered a group of supplementary alignments that couldn't be sorted unambiguously." + + "\nSee reads with the name: " + alignment.getReadName()); + return null; + } + //Is this the first or last in a set of supplementary reads by position along the original read. + // ex: + // --- Match / xxxx Clip + // original unaligned read: + // ----------------------------- + // split into 3 + // xxxxxx--------xxxxxxxxxxxxxx : not first or last + // ------xxxxxxxxxxxxxxxxxxxxxx: first + // xxxxxxxxxxxxxx--------------: last + + final boolean firstInRead = insertionIndex == 0; + final boolean lastInRead = insertionIndex == supplementaryAlignments.size(); + supplementaryRenderingInfo = new SupplementaryNeighbors(alignment, + firstInRead ? null : supplementaryAlignments.get(insertionIndex - 1), + lastInRead ? null : supplementaryAlignments.get(insertionIndex)); + return supplementaryRenderingInfo; + } else { + return null; + } } public String printString() { // chr6:43,143,415-43,149,942 (-) @ MAPQ 60 NM 763 - return chr + ":" + Globals.DECIMAL_FORMAT.format(start) + "-" + Globals.DECIMAL_FORMAT.format(start + lenOnRef) - + " (" + strand + ") = " + Globals.DECIMAL_FORMAT.format(lenOnRef) + "bp @MAPQ " + mapQ + " NM" + numMismatches; + // be sure to adjust start by + 1 because SATag is 1 based but IGV internal is 0 based + return chr + ":" + Globals.DECIMAL_FORMAT.format(start + 1) + "-" + Globals.DECIMAL_FORMAT.format(start + getLenOnRef()) + + " (" + strand.toShortString() + ") = " + Globals.DECIMAL_FORMAT.format(getLenOnRef()) + "bp @MAPQ " + mapQ + " NM" + numMismatches; } public static List parseFromSATag(String saTag){ return Arrays.stream(Globals.semicolonPattern.split(saTag)) - .map(SupplementaryAlignment::new) + .map(SupplementaryAlignment::fromSingleSaTagRecord) + .sorted(LEADING_CLIP_COMPARATOR) .collect(Collectors.toList()); } - int computeLengthOnReference(String cigarString) { + public static SupplementaryAlignment fromSingleSaTagRecord(String saTagRecord){ + //SA:(reference name, pos, strand, CIGAR, mapQ, NM;)+ + String[] tokens = Globals.commaPattern.split(saTagRecord); - int len = 0; - StringBuffer buf = new StringBuffer(); + //We have to canonicalize the chr name + String chr = tokens[0]; + Genome genome = GenomeManager.getInstance().getCurrentGenome(); + chr = genome == null ? chr : genome.getCanonicalChrName(chr); - for (char c : cigarString.toCharArray()) { + int start = Integer.parseInt(tokens[1]) - 1; // this is 1 based so subtract 1! + Strand strand = Strand.fromString(tokens[2]); + Cigar cigar = TextCigarCodec.decode(tokens[3]); + int mapQ = Integer.parseInt(tokens[4]); + int numMismatches = Integer.parseInt(tokens[5]); + return new SupplementaryAlignment(chr, start, strand, cigar, mapQ, numMismatches); + } - if (c > 47 && c < 58) { - buf.append(c); - } else { - switch (c) { - case 'N': - case 'D': - case 'M': - case '=': - case 'X': - len += Integer.parseInt(buf.toString()); - } - buf.setLength(0); - } + public int getCountOfLeadingClipping(){ + ClippingCounts counts = ClippingCounts.fromCigar(this.cigar); + return getCountOfLeadingClipping(counts, this.strand); + } + + public static int getCountOfLeadingClipping(final ClippingCounts counts, final Strand strand) { + switch(strand){ + case NONE: // if there's no strand it's not aligned, and we can assume it's in cycle order + case POSITIVE: + return counts.getLeft(); + case NEGATIVE: + return counts.getRight(); + default: throw new IllegalStateException(strand + " is not an expected value for strand"); } - return len; } + /** + * Sort by how the alignments were positioned in the original read. Sort by the amount of clipping on the strand in read order. + */ + public static final Comparator LEADING_CLIP_COMPARATOR = Comparator.comparingInt(SupplementaryAlignment::getCountOfLeadingClipping); + @Override public String getContig() { return chr; @@ -76,6 +169,109 @@ public int getStart() { @Override public int getEnd() { - return start + lenOnRef; + return start + getLenOnRef(); + } + + public int getLenOnRef() { + if(lenOnRef == null) { + lenOnRef = cigar.getReferenceLength(); + } + return cigar.getReferenceLength(); + } + + static class SupplementaryNeighbors { + final Alignment alignment; + final SupplementaryAlignment previous; + final SupplementaryAlignment next; + public SupplementaryNeighbors(Alignment alignment, + SupplementaryAlignment previous, SupplementaryAlignment next) { + this.alignment = alignment; + if(alignment.isNegativeStrand()){ + this.next = previous; + this.previous = next; + } else { + this.next = next; + this.previous = previous; + } + } + public SupplementaryAlignment previousIgnoreOrientation (){ + return alignment.isNegativeStrand() ? next : previous; + } + + public SupplementaryAlignment nextIgnoreOrientation (){ + return alignment.isNegativeStrand() ? previous : next; + } + + } + + public static class SupplementaryGroup { + private final Alignment alignment; + private final TreeSet readOrder; + private final TreeSet positionOrder; + + private final Adapter adapter; + + public SupplementaryGroup(Alignment alignment){ + final List supplementaryAlignments; + if( alignment instanceof SAMAlignment){ + supplementaryAlignments = ((SAMAlignment) alignment).getSupplementaryAlignments(); + } else { + final Object rawSATag = alignment.getAttribute(SAMTag.SA.name()); + supplementaryAlignments = rawSATag == null ? null : new ArrayList<>(parseFromSATag(rawSATag.toString())); + } + + this.alignment = alignment; + this.adapter = new Adapter(alignment); + final List combined = new ArrayList<>(supplementaryAlignments); + combined.add(adapter); + readOrder = new TreeSet<>(LEADING_CLIP_COMPARATOR); + readOrder.addAll(combined); + positionOrder = new TreeSet<>(SortOption.POSITION_COMPARATOR); + positionOrder.addAll(combined); + } + + public SupplementaryAlignment getNextInRead(SupplementaryAlignment alignment){ + return readOrder.higher(alignment); + } + + public SupplementaryAlignment getPreviousInRead(SupplementaryAlignment alignment){ + return readOrder.lower(alignment); + } + public SupplementaryAlignment getNextPosition(SupplementaryAlignment alignment){ + return positionOrder.higher(alignment); + } + + public SupplementaryAlignment getPreviousPosition(SupplementaryAlignment alignment){ + return positionOrder.lower(alignment); + } + + public Adapter getAdapter() { + return adapter; + } + + public Iterator iterateInReadOrder(){ + return readOrder.iterator(); + } + + public Iterator iterateInPositionOrder(){ + return positionOrder.iterator(); + } + + public Stream streamInReadOrder(){ + return readOrder.stream(); + } + public Stream streamInPositionOrder(){ + return positionOrder.stream(); + } + + public int size(){ + return readOrder.size(); + } + + private static class Adapter extends SupplementaryAlignment{ + public Adapter(Alignment a) { + super(a.getChr(), a.getStart(), a.getReadStrand(), a.getCigar(), a.getMappingQuality(), a.getAttribute(SAMTag.NM.name()) == null ? 0 : (int)a.getAttribute(SAMTag.NM.name())); + } + } } -} +} \ No newline at end of file diff --git a/src/main/java/org/broad/igv/ui/AlignmentDiagramFrame.java b/src/main/java/org/broad/igv/ui/AlignmentDiagramFrame.java new file mode 100644 index 0000000000..c1df4c3feb --- /dev/null +++ b/src/main/java/org/broad/igv/ui/AlignmentDiagramFrame.java @@ -0,0 +1,234 @@ +/* + * The MIT License (MIT) + * + * Copyright (c) 2007-2015 Broad Institute + * + * Permission is hereby granted, free of charge, to any person obtaining a copy + * of this software and associated documentation files (the "Software"), to deal + * in the Software without restriction, including without limitation the rights + * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell + * copies of the Software, and to permit persons to whom the Software is + * furnished to do so, subject to the following conditions: + * + * The above copyright notice and this permission notice shall be included in + * all copies or substantial portions of the Software. + * + * + * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR + * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, + * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE + * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER + * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, + * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN + * THE SOFTWARE. + */ + +package org.broad.igv.ui; + +import htsjdk.samtools.SAMRecord; +import htsjdk.samtools.SAMRecordSetBuilder; +import htsjdk.samtools.SAMTag; +import htsjdk.samtools.util.Locatable; +import org.broad.igv.feature.Strand; +import org.broad.igv.feature.genome.ChromosomeNameComparator; +import org.broad.igv.sam.*; +import org.broad.igv.ui.color.ColorPalette; +import org.broad.igv.ui.color.ColorUtilities; +import org.broad.igv.util.ChromosomeColors; +import org.broad.igv.util.Pair; + +import javax.swing.*; +import java.awt.*; +import java.awt.geom.Ellipse2D; +import java.awt.geom.Point2D; +import java.awt.geom.QuadCurve2D; +import java.awt.geom.Rectangle2D; +import java.util.List; +import java.util.*; +import java.util.stream.Collectors; +import java.util.stream.StreamSupport; + + +public class AlignmentDiagramFrame extends JFrame { + + public AlignmentDiagramFrame(Alignment alignment, Dimension dimension) { + + setSize(dimension); + + JPanel readPanel = new JPanel(); + BoxLayout boxLayout = new BoxLayout(readPanel, BoxLayout.Y_AXIS); + readPanel.setLayout(boxLayout); + ReadDiagram diagram = new ReadDiagram(alignment); + readPanel.add(diagram); + diagram.setVisible(true); + setAlwaysOnTop(true); + + this.add(readPanel); + + + } + + private static class ReadDiagram extends JComponent { + + public static final int BORDER_GAP = 30; + public static final int BETWEEN_ALIGNMENT_GAP = 15; + public static final int BETWEEN_CONTIG_GAP = 10; + public static final int ALIGNMENT_HEIGHT = 10; + + SupplementaryAlignment.SupplementaryGroup toDraw; + + public ReadDiagram(Alignment alignment) { + this.toDraw = new SupplementaryAlignment.SupplementaryGroup(alignment); + } + + + @Override + protected void paintComponent(final Graphics g) { + super.paintComponent(g); + g.drawString("Hello", 0, 0); + draw((Graphics2D) g, toDraw); + } + + private void draw(final Graphics2D g, SupplementaryAlignment.SupplementaryGroup toDraw) { + int totalWidth = getWidth(); + final List contigs = toDraw.streamInPositionOrder() + .map(SupplementaryAlignment::getContig) + .filter(Objects::nonNull) + .sorted(ChromosomeNameComparator.get()) + .distinct() + .collect(Collectors.toList()); + + final int totalAlignedBases = toDraw.streamInPositionOrder() + .mapToInt(Locatable::getLengthOnReference) + .sum(); + + String lastContig = contigs.get(0); + final int mid = getHeight() / 2; + double lastPosition = BORDER_GAP; + + float alpha = 0.75f; + int type = AlphaComposite.SRC_OVER; + Composite alignmentAlphaComposite = AlphaComposite.getInstance(type, alpha); + g.setComposite(alignmentAlphaComposite); + g.setColor(Color.LIGHT_GRAY); + + Map> positions = new LinkedHashMap<>(); + int arrowPxWidth = 5; + for (SupplementaryAlignment sa : (Iterable) toDraw::iterateInPositionOrder) { + // System.out.print(sa.toString()); + final int length = sa.getLengthOnReference(); + double fractionOfWhole = (double) length / totalAlignedBases; + double availableSpace = totalWidth - (2 * BORDER_GAP + (toDraw.size() - 1) * BETWEEN_ALIGNMENT_GAP + (contigs.size() - 1) * BETWEEN_CONTIG_GAP); + double spaceToUse = fractionOfWhole * availableSpace; + final String newContig = sa.getContig(); + if (lastPosition != BORDER_GAP && !Objects.equals(lastContig, newContig)) { + lastPosition += BETWEEN_CONTIG_GAP; + } + + lastContig = newContig; + int y = mid - ALIGNMENT_HEIGHT; + int h = ALIGNMENT_HEIGHT; + int start = (int) lastPosition; + int end = (int) (lastPosition + spaceToUse); + /* + 1 2 + 0 <|=======|> 3 + 5 4 + */ + final int startAdjusted = start - (sa.getStrand() == Strand.NEGATIVE ? arrowPxWidth : 0); + final int endAdjusted = end + (sa.getStrand() == Strand.POSITIVE ? arrowPxWidth : 0); + positions.put(sa, new Pair<>(startAdjusted, endAdjusted)); + int[] xPoly = {startAdjusted, start, end, endAdjusted, end, start}; + int[] yPoly = {y + h / 2, y, y, y + h / 2, y + h, y + h}; + Polygon blockShape = new Polygon(xPoly, yPoly, xPoly.length); + lastPosition = end; + lastPosition += BETWEEN_ALIGNMENT_GAP; + g.fill(blockShape); + g.draw(blockShape); + if (sa == toDraw.getAdapter()) { + Color original = g.getColor(); + g.setColor(Color.DARK_GRAY); + g.draw(blockShape); + g.setColor(original); + } + } + + toDraw.streamInReadOrder() + .forEachOrdered(sa -> { + SupplementaryAlignment next = toDraw.getNextInRead(sa); + if (next != null) { + final Pair currentPos = positions.get(sa); + final Pair nextPos = positions.get(next); + int from = sa.getStrand() == Strand.NEGATIVE ? currentPos.getFirst() : currentPos.getSecond(); + int to = next.getStrand() == Strand.NEGATIVE ? nextPos.getSecond() : nextPos.getFirst(); + g.setStroke(new BasicStroke(2, BasicStroke.CAP_BUTT, BasicStroke.JOIN_MITER)); + int centerX = (from + to) / 2; + int width = Math.abs(from - to); + int height = (int) ((double) width / getWidth() * getHeight()); + int centerY = mid - ALIGNMENT_HEIGHT / 2; + g.setRenderingHint(RenderingHints.KEY_ANTIALIASING, RenderingHints.VALUE_ANTIALIAS_ON); + g.setColor(Color.GRAY); + g.drawArc(centerX - width / 2, centerY - height / 2, width, height, 0, 180); + drawPoint(g, to, centerY); + } + }); + + positions.keySet(). + stream() + .collect(Collectors.groupingBy(SupplementaryAlignment::getContig)) + .forEach((contig, alignments) -> { + int start = positions.get(alignments.get(0)).getFirst(); + int end = positions.get(alignments.get(alignments.size() - 1)).getSecond(); + g.setColor(ChromosomeColors.getColor(contig)); + final FontMetrics fontMetrics = g.getFontMetrics(); + final int labelWidth = fontMetrics.stringWidth(contig); + // s----label-----e + final int lineY = mid + 15; + final int LABEL_GAP = 2; + if (labelWidth + 2 * LABEL_GAP < end - start) { + final int leftLineEnd = (start + end - labelWidth) / 2 - LABEL_GAP; + final int rightLineStart = (start + end + labelWidth) / 2 + LABEL_GAP; + g.drawLine(start, lineY - 2, start, lineY + 2); + g.drawLine(end, lineY - 2, end, lineY + 2); + g.drawLine(start, lineY, leftLineEnd, lineY); + g.drawString(contig, leftLineEnd + LABEL_GAP, lineY + fontMetrics.getHeight() / 3); + g.drawLine(rightLineStart, lineY, end, lineY); + } else { + g.drawString(contig, start, lineY + fontMetrics.getHeight() / 3); + } + }); + } + + private static void drawPoint(Graphics2D g, int x, int y) { + g.draw(new Ellipse2D.Double(x - 1, y - 1, 2, 2)); + } + + private static void writeContigName(final Graphics g, final String contig, final double x, final int y) { + Color originalColor = g.getColor(); + try { + g.setColor(ChromosomeColors.getColor(contig)); + g.drawString(contig, (int) x, y); + } finally { + g.setColor(originalColor); + } + } + } + + + + public static void main(String[] args){ + final SAMRecordSetBuilder samRecords = new SAMRecordSetBuilder(); + samRecords.addPair("read1", 13, 50255292, 50255400 ); + final List records = new ArrayList<>(samRecords.getRecords()); + + final SAMRecord record = records.get(0); + String read = "m141213_163442_00118_c100750562550000001823151707081585_s1_p0/6849/0_5821\t2064\t14\t50255292\t38\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\t*\t0\t0\tATTCAAGGCACCAGCAGATTCTTGTCTGAATAGGGCTTGCCCCTCAAAGATTGGTACCTATAAGGCTGAGGTTGGGTGGCTCATACCTGTAATCCCAGCACTTTGGCGAGGCCAAGGTCGGGGGGATCCTTGGCCCAGGAGATTCGACACCAGCCTGCGGCAACAAGGGAGACCTCAATCTTAAAAAAAAGATGGCACCTTATTGCAGTGTACCTCACGTGGCAACAGGGGCACTCATCCTCATTCATGACACAGAGGCCCCTCGTTGACTTCACTTCCTAAAGGCCACTCTTAAATACCATCCACACTGGGCTATTTAGGTTCCCATATATGAATGTGGGGATAATTAACTGTAGAACCATAGCAGCCGTAACTCATGAAAGAACATTAATAAGTTTAACACCTCAGTGAGTATTTGTTGAGTCGTAGGATTAACAGAACATAAGGTTATTTGTAACAAATTTTTTAAAAAGACCACCAAAAATGGTTTTAGTCATTACTAAACCAACAGTGTCAGCAGGAAAAAAAGAAAATAGTAATTACAGAATTCCCGTAAGACATTACCTGCCATAAACTGGAGCACACAATTCATAACTCAAGCTTGACATAAAAAAATGCTGGTGGCAAAAGTAAAATATTTTTATTGCCTCTATTTTAGCCGTTATTAAAAACATAAACAGGCTGGTGACATGGTGTCTTACAGCCTGTATCCCAACACTTTGGGAGGCCAAGGGCAGGCGGAATTCACGGAGATCAGGACGATTTCAAGACCAGCACTGGCCAACAATGGTTGAACCCCAATTCACCACTTAAAATACCAAAATAAGCCAGGCGTAGATGGCGCATTTGCTTAGTAATCCCAAGCTACTCAGAAGGCTGAGGCGGATAATTGCTTGAATCACGGGAGGTGGCAGGTTGCCAGTGAGCTGAGATTGTGCCACTTGCACTCCAGCCTGGGACAAGAGCCGAGCGACTGCCTCAAAAAAAAAAAAAAAAAAAAAAAAATTCAAAGAGAGGTAAATTTCATGTAAGAAGATATACAGCAAGGCGTGGGCTTGGCTGTACTAGTTAACAAAACAATATGTGTGTAGGCGATGTGTCTGTCTTAGCTGGAAAATATTCGTCAGTGGTTTTGTAACCAGGGTACTTGGCTCTTTAAATGGTGTACTCGATGTTCAACATTATGTACGACCATATTGGTGTATAGATCGTGAGTGTGTTAGGAGTGTGGATGAAATTTATTTGTGGGGTGTCTTCAACAGACTGTTTCCAATTATTCTCTGTGTTGGTTTTTTGTGTGTGTTGAGATGGAGTACTCGCATCTGTCGACCAGGCTGGAGTGCAATGCACAATCTCGGACTCACTGACACAGCTCTGCCTCCAGAGTTCACGACTATTCTGCCTTCAGCCTTCCCAGTAGCTGAGGGGGGACTAACAGAGGCGCTCACCACCACACCCAGGCTAATTTCTTTTGTTATTTTTTTAGTAGAGACAGCGGTTTCACCACGTATAGCCAGGCATGGTTCTCAATTTCCTGACCTTTGTTGATCTGCCTGCCTGGCCTCTCAAAAGATTGCTGGATTACAGGCGTGAAGCCACCGTGCCAGGCCTCTCATTCTTATCTTTTTTTTTATATTAACTCAGTTCCATCAGAATCCAAAGCCTCTCCGCTTAAAATGATACTTCCTAAAGCTCTGTAAACCAAATTGTTATCAGCTAATCGAAGTACTAACCATTCTATTTGGCTTAGCATAAGATACGTGTGTGGATGTGGTAGTGTCTCGTGGTGTGTGTTGGTTGTATTGTGATGGGGGGGTGTCACTTGTCTTGTGTGGTGTACTGTGGTGCCAGTGTGGTTCCTGACTCCAGGAGGGTAATCACCCTAATTAAGAAGCAGGAAGCCACAAAGTAGCCATACCTTAGTCATCATGTGGATCATCTTACAGCAAATCTCTGTTAACTTCATAACGTTATAAACCTCAAGGAACGGAGTATTCTATTTTCTTAATGTTGTCAGAGGCACCCTCTGTCCCACCTCTAGGTTTTCTGGGGCTGTTTCTTCACAGGTTTGGTCTTTGTTTTTCCTTGGTTCTTCCCCTTCTTCCCTTTTTTCTTCTTTGTTTGAACCTGACCAGACTTTAATTCAGTAGAGGAAAACATCAGTCCAGTGTTGTATTTTTCAATACTGTGGGAATATTTTTACAAGATGTATTGAATTGCCTGTCATACGGCATAGGCAGGTGGGCTAGATGGCCCATTTTGAAAAAGCAAAAGAAAAAAATTGTTTTTAGAATGTTATTTACCCCCAGCATACCTTCATGATAAGTTCACGGTCTTCTTCATCTGGTCTTTGTATTTTTCTTTCATTTTTTCATTTGACTCTTACAAATTCAGAAGATTTTGGTTGAATATTTCAGCAGCAAAAAAAATGTCAACAAATACTTTTGCAAGAAATGGTGTTACTTTATTATTCTCTGTCAAAATGCTTACTGAATAAGGCAACCATATATAATAAACATATTGAGGCTACAAGTTGGATAACTGAAGGTCCTTCATCTTCAGGAATTTACATAGGGTGGAGAGAAGATTACACAGAGAAGTTAAGAATACTTAACAAACATTAAAGAAACTGTTGAGGAAGAAATAAAGTAAAACAGTATTGGAAAAGCAGTCTAATCCTCCTGCCTCAGCCTCCGAGTAGCTGGGGACTACAAGCGCTGCTGCCATGCCCGGCTAATTTTCTGTTATTTTTTAGTAGAGATGGGGTTATTCCACTGTATTAGCCAGGATGGTCTCCAATCTCCTGACTTGTGATTGCCCGCCTCGCCTCCCAAATTGCTGCGGATTTAACAGCGATGAGCCACCACTGCACCGGCCAAAAGTTTTTAAAAAATATAGTCTCAAGAAAGCATAACCATACTGTTAGTTGTGTTTAACTACTAAAAAATGAGGGGGTCCAAAATTTGGACTTCTGAGCAACTAAACAAAATAGAAACATGATCGTTTATATGGATCTTTCCAATGAGCCTATTAACTTTTCTCCTTCTTTAAATCCAATGGTTTAATTTTCAATATTTTCATTTATCAAGTGATAACATTAAGTAAACAAAAATTAAAATCACATTTTTTACCTTAGGTTGTGAGCTGATTGTGTCCTCCAAAAGATAGTGATTGAGGACAATCTCCACGTTTACCTATGAAGTGTGGCCTTATTTGGTTGATAGTGTCTTTCAGATGTAAGCAAGTTAAAATGATATTCTATACTGGATTAGGGTGGGCCCTTAAATCCAACAACTTCTGACATTATGAGAAAGGCATGTTGGGAAGAACATCGAGACAGAAATACACAATTGAAACATGAAATTGTTGACAGAGCAGGACTTGCCATGCTCACTGTAGCCAGGAAATGCTGAGGACCACCCGCAGCCACAGGAAGCTCCACAGAGGACAAGAAGAATTTTCCTCTACCAAGAACCTTCAAGAAAAGCCAGGTTCTTGTGGTGGGCGTGCTTTCATTCATACTTGCAGGTCTTCAAAACTGTGAGGCAATAATTTCTGTTGCTTTTAAAGCCACTCGGTTTGTGGTCATTTGCCACGCGCCTAGGAATCAAAACTAACTATCAACTGGCCAACTTTCGGTTGTTTTTATTTATTTTTTATTTTCATTTTTATTTTATTTTTTTATTTGTTTGAGATGGAGTCTCCCTTGTTGCCCCAGCTGGAGTGCAGAGTGGGCTATGATCCTCAAGCTCACTTGCAACCCCATGGGCCTCCTGGGTTCAAGTGATTCTCCTTGCTCAGCTCCTGAGTAGCTGGGATATACAGGCACACGCCACCATGCGCGGCTAAATTTTTTGTATTTGAGTAGGACGAGGGGTTCACCTGTTGGCCCAAGGCTTTGGATTCTCGAAACTCCTGACGTCAAAAGTGGATCCGCCCACCTTGGCTTCCAAAAGTGCTGGATTTACAGGCGAAAAGCCACTGCACCCAGCCTGTTTTTAATTAACACCATTTTGGTTTGAAATGCTAGAAGGAATTGGGACATTTAACATATACTTTCATTAGAGAGTAATTTTTTTATTGTAAGATATTAATTTTCTGTTTTTCTTTTTCATCATCTCTTTTTTCTAAAGTTTTCAATGTTGAGCTTGTATATCTTTTTGCAATAACAAAAACTTTCATTTAAAAACACTATTGTCACCATTATTTAACATTTTTCTCGTAAGTTTCTAGCGGTAAGTGGCTAAGAAATGGATGTCGGGTTTGATTTTAACAGCTCCCTGGGTCGGGGAGAACAGGGTTGGACTTGAAATCACAGGAAACATGAATCTTCAGTCCCAAGCTGTGCCATTTCCAGCTGTATGATCTTGAGTAAGCCACTGAACTTTTCGTAAACCTCAAGTTTCCCCCACCTCCCTATGATGTTGTTTCTATTGGGCTCTTCAATTTTCTACCACCGTGTGCCCTTTAAAGAAGGATGAGAGCAAGAGGGAAACAAATGTCAAACATTAATTGCATTTTTAATACAGTTCACTGGAGGATGACCCTGGGGAAGAGGGATTGAGGGAAATCAATTCTGGTGATGAAAACAATAATCGAGAATTACAAGAAGGGTCACAGCTTTAAATCTGGATTAGAGAGAAAAATGCCTCTGCATTTTTCTTTGCCTTTGTGGGGTTCAGTCAGACTGTTACTTGATTCAATGTTTACTGTGATTCAAACAGTTCTGAGGGGAAATGCTTTGTGTGTCATCAACTATTACAGACACTGAGGGGACAGAGATGTTCCAGACATAACCCTGTTTTCCTTCATGTGGGCTTCTCTTGTGAGTGGGTGAAGGGTGGGGTCAAAGTACTAGATGGATTTTTTTTAAATTTTTTATATTGTACTTTAAGTTTTAGGGGTACATGTGCACAAATGTACAGGTTTGTTACATATATTTACATGTGCCCATGTTTGGTGTGCTGCAACCATTAACTCGTCAGTTAACATTTAGGGTTATAATCTCCTAATGCTATTCTACCCCGCTCCCGCACCCATAACAGGCTTCTGTTATTGATGTTTCCCTTTTCTGTGTCCATGTGTTTCTCATTGTTCAATTCCACCTGTTGAGTGAGACATGCCAGTGTTTTTGGTTTTTTGTCCTTGTTTGATGTTTGCGGAGAATGAATGGTTTTCCAGCTTCATCCATGTCCCTACAAAGACATGAAAATTCATTTAAGATGGATTTTTTTAAATGACAAAGCAGAATCAGAAAAAATGGCTAAGAGAGGATGAATTGGCTGAGCCTTCTTTTCCATGCATTGGTGTCACCTTTATAAAGGATTGTGAAAATGTCAAGGCAGGGCAGCTGATCTTTTATTTATTGGTTTTCATTACAGGTGGACCTGGCCAGAAGCCCTTGGGAAACTGGCGGTGCGCTACGGGTAATTTATTTCTAGGCACCTTCAAAGGAGAAAAGGGCAGTGTCCTCCCTTCTCAACTGGATATTGTGCTAGACCATAGTTGCATACCGCCCACAAGCCCCCAAGCATCAGCTGGCCTGGCATCCCTGCCCAGAAGGGCCCATTGCCACTGGTCTTTCCACAAAAACCTGAGCCTTAGGCCAAATTAAAAAGCCACTTGTAGGCATTTTTACCACTTGAATTTACAGCCAAGAGGAAAATCCAACCATGGGGAAAAAGATCCACTGGCTTTTGGCAATTTTCCAAACTTTAAAAACCCCCAAAAGGGCCAAAAAAGGCAAAAACTACCCCAAAGGCCAAAAAGGCTATTAGGAACATTACAAGGAAAAAAAGTCTTTGATATTTCTTTTCTTTGT\t*\tSA:Z:17,64042266,-,2676S17M1D10M1I14M1D26M1I2M1I20M2I1M1I23M1I11M1D8M1D10M1D16M1I3M1I3M1D1M1I4M1I11M1I2M1I6M1D10M1I11M1D3M1D11M1D8M3I2M1I14M1I7M4I12M1I3M1I9M1D13M1D23M1I4M1I13M1D12M1I38M1I11M1I5M1D11M1I17M1I14M1D12M1D8M1I2M1I3M1D12M1I2M2I8M1I19M1D1M2D11M1D25M1I2M1I21M1I28M1I4M1D4M1I1M2I4M1I22M1I5M1I3M1D3M1I9M2I6M1I9M1D2M1D4M1D11M1D2M1I18M1D2M1I13M1I23M1I17M1I7M1I2M1D4M1I21M1I25M1D12M1I3M1I29M1D2M1D1M1D6M1D8M1I8M1D17M1D13M1I54M1D5M1I4M1D12M2I1M1I3M1I5M1I3M1I7M1I9M1I1M2I25M1I2M1D5M1D18M1I27M1I2M1I8M1D9M1D3M1I11M1D6M1I2M1I4M2I3M2I5M1I13M2I4M1I30M1D3M1I9M1I30M2D4M1D4M1D9M1D5M1I5M1I3M2I6M1I15M1D20M1I3M1I13M5I18M2I10M1I7M1D1M1I38M1D39M1I5M1I6M1588S,36,188;14,50254065,-,2S19M1D7M1I2M1D8M2I9M1I8M1I1M1I6M1I2M1I2M1D32M1I12M1I7M1D5M1D9M1I15M1I7M1D11M1I5M1I28M1I13M1I12M1I15M1I2M1D1M1I6M1I19M1D2M1D1M1D5M1I8M1I9M1I22M1D3M2D6M1I6M1D5M1I9M1I2M1I1M1I22M2I10M1D12M1I6M1I24M1I4M1I5M2I4M1I5M4I2M1I6M1D8M1I10M2D7M1I8M1D18M1I7M1I4M1D5M1I9M1I2M1D6M1I12M1I18M1D27M1I1M1D7M1D1M1D11M1D15M1I13M1I1M1I3M1I9M1I6M1D23M1I9M1I1M1I3M1D12M1I1M2I12M1I9M1I4M1I2M1D6M1I1M1I33M1I9M2I5M1I8M1I20M1D18M1I10M1I6M1I22M1I16M1I6M3I3M2D9M5I23M1I3M1D10M1D21M1I5M1I7M1I8M1I14M7I8M4I1M1I15M1I5M3D31M1I15M1I26M4624S,38,166;8,123906302,+,157S3M1I2M1I8M1I2M1I2M1I3M1I4M1D14M1I2M2I3M1I1M1I7M1I5M1D13M2I3M1I7M1I2M1I9M2D5M1D11M1I2M1D10M2I4M1I3M1I5M1D5M1I4M1I3M1D4M1I5M1I45M1I4M1I12M1D3M1I1M1I4M2I27M1I3M1D4M1I3M1I5M1I2M1D4M1I6M1D13M1I12M1I9M1I11M1I18M2I4M1I12M1D7M1I40M2I3M1I1M1I8M1D26M1I6M1I15M1D3M2I16M2I7M1I5M1D8M1I6M1D16M1I22M1I6M1I3M1D1M1D3M1I10M1D6M1D10M1I1M1D16M1I3M1I1M1I3M1I35M1I5M1I33M1I13M1I22M2I22M1D5M1I1M1D4M1D21M1I15M1I3M1I5M1I9M1D3M1I10M1D13M1D2M1I4M1I3M1D8M2I5M1D2M3I3M1D20M2I10M1I16M1I5M1I1M2I4M2I2M1I14M1D22M1I7M1I4M1I13M1I1M1I5M1D5M1D22M1I7M1I13M1I17M1I15M1I26M1I1M1D11M2D8M1D20M1I7M1D4M1I8M1D8M4409S,30,166;8,126480844,-,4239S17M1D6M1I30M1I11M1D4M1I7M1I11M1I9M1I50M1I8M1I10M1411S,2,11;\tKB:f:24.508675\tSB:f:24.508675\tID:i:150690\tMD:Z:24C15G1^G31^C31^A101C24^T17^G36^G11T6^C28^C3^T11^G9A30A1^C19C0A3T4C1T2^A2T3A1T1^C3A2^A0C3^CC0A1T1A1^AA0T0A3T1^A0C14T0T1^A1C4^A0A0A2C9^GG1^A9^T29^C47^G13G92C2^C115A5^A125^C23^T11T10A16^G5^A33^A30^A39^A2^A7^A28^A9^C39^A5^A51^A7\tQE:i:2676\tXE:i:1426\tXI:f:0.8567\tNM:i:203\tXR:i:1374\tAS:i:1426\tQS:i:1302\tXS:i:0\tCV:f:23.60419\tSV:i:2"; + String saTag = "17,64042266,-,2676S17M1D10M1I14M1D26M1I2M1I20M2I1M1I23M1I11M1D8M1D10M1D16M1I3M1I3M1D1M1I4M1I11M1I2M1I6M1D10M1I11M1D3M1D11M1D8M3I2M1I14M1I7M4I12M1I3M1I9M1D13M1D23M1I4M1I13M1D12M1I38M1I11M1I5M1D11M1I17M1I14M1D12M1D8M1I2M1I3M1D12M1I2M2I8M1I19M1D1M2D11M1D25M1I2M1I21M1I28M1I4M1D4M1I1M2I4M1I22M1I5M1I3M1D3M1I9M2I6M1I9M1D2M1D4M1D11M1D2M1I18M1D2M1I13M1I23M1I17M1I7M1I2M1D4M1I21M1I25M1D12M1I3M1I29M1D2M1D1M1D6M1D8M1I8M1D17M1D13M1I54M1D5M1I4M1D12M2I1M1I3M1I5M1I3M1I7M1I9M1I1M2I25M1I2M1D5M1D18M1I27M1I2M1I8M1D9M1D3M1I11M1D6M1I2M1I4M2I3M2I5M1I13M2I4M1I30M1D3M1I9M1I30M2D4M1D4M1D9M1D5M1I5M1I3M2I6M1I15M1D20M1I3M1I13M5I18M2I10M1I7M1D1M1I38M1D39M1I5M1I6M1588S,36,188;14,50254065,-,2S19M1D7M1I2M1D8M2I9M1I8M1I1M1I6M1I2M1I2M1D32M1I12M1I7M1D5M1D9M1I15M1I7M1D11M1I5M1I28M1I13M1I12M1I15M1I2M1D1M1I6M1I19M1D2M1D1M1D5M1I8M1I9M1I22M1D3M2D6M1I6M1D5M1I9M1I2M1I1M1I22M2I10M1D12M1I6M1I24M1I4M1I5M2I4M1I5M4I2M1I6M1D8M1I10M2D7M1I8M1D18M1I7M1I4M1D5M1I9M1I2M1D6M1I12M1I18M1D27M1I1M1D7M1D1M1D11M1D15M1I13M1I1M1I3M1I9M1I6M1D23M1I9M1I1M1I3M1D12M1I1M2I12M1I9M1I4M1I2M1D6M1I1M1I33M1I9M2I5M1I8M1I20M1D18M1I10M1I6M1I22M1I16M1I6M3I3M2D9M5I23M1I3M1D10M1D21M1I5M1I7M1I8M1I14M7I8M4I1M1I15M1I5M3D31M1I15M1I26M4624S,38,166;8,123906302,+,157S3M1I2M1I8M1I2M1I2M1I3M1I4M1D14M1I2M2I3M1I1M1I7M1I5M1D13M2I3M1I7M1I2M1I9M2D5M1D11M1I2M1D10M2I4M1I3M1I5M1D5M1I4M1I3M1D4M1I5M1I45M1I4M1I12M1D3M1I1M1I4M2I27M1I3M1D4M1I3M1I5M1I2M1D4M1I6M1D13M1I12M1I9M1I11M1I18M2I4M1I12M1D7M1I40M2I3M1I1M1I8M1D26M1I6M1I15M1D3M2I16M2I7M1I5M1D8M1I6M1D16M1I22M1I6M1I3M1D1M1D3M1I10M1D6M1D10M1I1M1D16M1I3M1I1M1I3M1I35M1I5M1I33M1I13M1I22M2I22M1D5M1I1M1D4M1D21M1I15M1I3M1I5M1I9M1D3M1I10M1D13M1D2M1I4M1I3M1D8M2I5M1D2M3I3M1D20M2I10M1I16M1I5M1I1M2I4M2I2M1I14M1D22M1I7M1I4M1I13M1I1M1I5M1D5M1D22M1I7M1I13M1I17M1I15M1I26M1I1M1D11M2D8M1D20M1I7M1D4M1I8M1D8M4409S,30,166;8,126480844,-,4239S17M1D6M1I30M1I11M1D4M1I7M1I11M1I9M1I50M1I8M1I10M1411S,2,11"; + record.setAttribute(SAMTag.SA, saTag); + final Alignment samAlignment = new SAMAlignment(record); + + AlignmentDiagramFrame frame = new AlignmentDiagramFrame(samAlignment, new Dimension(500, 100)); + frame.setVisible(true); + + } +} diff --git a/src/main/java/org/broad/igv/ui/IGV.java b/src/main/java/org/broad/igv/ui/IGV.java index 0f68aad6ee..89f0e7bcd9 100644 --- a/src/main/java/org/broad/igv/ui/IGV.java +++ b/src/main/java/org/broad/igv/ui/IGV.java @@ -1408,14 +1408,14 @@ private boolean isAnnotationFile(String format) { public void sortAlignmentTracks(SortOption option, String tag, final boolean invertSort) { - sortAlignmentTracks(option, null, tag, invertSort); + sortAlignmentTracks(option, null, tag, invertSort, null); } - public void sortAlignmentTracks(SortOption option, Double location, String tag, boolean invertSort) { + public void sortAlignmentTracks(SortOption option, Double location, String tag, boolean invertSort, Set priorityRecords) { List alignmentTracks = getAllTracks().stream() .filter(track -> track instanceof AlignmentTrack) .map(track -> (AlignmentTrack)track) - .peek(track -> track.sortRows(option, location, tag, invertSort)) + .peek(track -> track.sortRows(option, location, tag, invertSort, priorityRecords)) .collect(Collectors.toList()); this.repaint(alignmentTracks); } @@ -2288,7 +2288,7 @@ private void repaint(final JComponent component, Collection tra try { for (ReferenceFrame frame : FrameManager.getFrames()) { for (Track track : trackList) { - if (track.isReadyToPaint(frame) == false) { + if (!track.isReadyToPaint(frame)) { track.load(frame); } } @@ -2313,11 +2313,11 @@ private void repaint(final JComponent component, Collection tra return; } - List futures = new ArrayList(); + List> futures = new ArrayList<>(); for (ReferenceFrame frame : FrameManager.getFrames()) { for (Track track : trackList) { - if (track.isReadyToPaint(frame) == false) { + if (!track.isReadyToPaint(frame)) { futures.add(CompletableFuture.runAsync(() -> track.load(frame), threadExecutor)); } } @@ -2330,9 +2330,9 @@ private void repaint(final JComponent component, Collection tra component.repaint(); }); } else { - // One ore more tracks require loading before repaint. Load all needed tracks, autscale if needed, then - // repaint. The autoscale step is key, since tracks can be grouped for autoscaling it is neccessary that - // all data is loaded before any track is repainted. Otherwise tracks be loaded an painted independently. + // One or more tracks require loading before repaint. Load all needed tracks, autoscale if needed, then + // repaint. The autoscale step is key, since tracks can be grouped for autoscaling it is necessary that + // all data is loaded before any track is repainted. Otherwise tracks be loaded and painted independently. final CompletableFuture[] futureArray = futures.toArray(new CompletableFuture[futures.size()]); WaitCursorManager.CursorToken token = WaitCursorManager.showWaitCursor(); @@ -2353,6 +2353,7 @@ private void repaint(final JComponent component, Collection tra }); return null; }).exceptionally(ex -> { + WaitCursorManager.removeWaitCursor(token); log.error("Error loading track data", ex); isLoading = false; pending = null; diff --git a/src/main/java/org/broad/igv/ui/panel/DataPanel.java b/src/main/java/org/broad/igv/ui/panel/DataPanel.java index b7a4606cbe..51e77e72d8 100644 --- a/src/main/java/org/broad/igv/ui/panel/DataPanel.java +++ b/src/main/java/org/broad/igv/ui/panel/DataPanel.java @@ -797,7 +797,7 @@ public void run() { } /** - * A utility class for sceduling single-click actions "in the future", + * A utility class for schedueling single-click actions "in the future", * * @author jrobinso * @date Dec 17, 2010 diff --git a/src/main/java/org/broad/igv/ui/panel/FrameManager.java b/src/main/java/org/broad/igv/ui/panel/FrameManager.java index 85c46c0d53..dd36db8352 100644 --- a/src/main/java/org/broad/igv/ui/panel/FrameManager.java +++ b/src/main/java/org/broad/igv/ui/panel/FrameManager.java @@ -25,6 +25,7 @@ package org.broad.igv.ui.panel; +import com.jidesoft.utils.SortedList; import org.broad.igv.event.GenomeChangeEvent; import org.broad.igv.event.IGVEventBus; import org.broad.igv.event.IGVEventObserver; @@ -65,7 +66,7 @@ public class FrameManager implements IGVEventObserver { return s0 - s1; }; - private static List frames = new ArrayList(); + private static List frames = new ArrayList<>(); private static ReferenceFrame defaultFrame; public static final String DEFAULT_FRAME_NAME = "genome"; diff --git a/src/main/java/org/broad/igv/ui/util/ApplicationStatusBar.java b/src/main/java/org/broad/igv/ui/util/ApplicationStatusBar.java index 5a9b7265b1..952411d4ed 100644 --- a/src/main/java/org/broad/igv/ui/util/ApplicationStatusBar.java +++ b/src/main/java/org/broad/igv/ui/util/ApplicationStatusBar.java @@ -27,15 +27,19 @@ package org.broad.igv.ui.util; import com.jidesoft.swing.JideBoxLayout; +import org.broad.igv.event.StopEvent; import org.broad.igv.logging.*; import org.broad.igv.oauth.OAuthProvider; import org.broad.igv.ui.FontManager; import org.broad.igv.event.IGVEventBus; import org.broad.igv.event.IGVEventObserver; +import org.broad.igv.ui.IGV; //import org.broad.igv.event.StopEvent; import javax.swing.*; import java.awt.*; +import java.awt.event.ActionEvent; +import java.awt.event.ActionListener; import java.text.NumberFormat; import java.util.TimerTask; @@ -78,6 +82,7 @@ private void initialize() { stopButton = new JButton(); stopButton.setBorder(BorderFactory.createLineBorder(Color.black)); + stopButton.addActionListener(e -> IGVEventBus.getInstance().post(new StopEvent())); stopButton.setIcon(new javax.swing.ImageIcon( getClass().getResource("/toolbarButtonGraphics/general/Stop16.gif"))); stopButton.setMaximumSize(new java.awt.Dimension(16, 16)); diff --git a/src/main/java/org/broad/igv/ui/util/UIUtilities.java b/src/main/java/org/broad/igv/ui/util/UIUtilities.java index 2cae9ccd99..c1aab4cfd1 100644 --- a/src/main/java/org/broad/igv/ui/util/UIUtilities.java +++ b/src/main/java/org/broad/igv/ui/util/UIUtilities.java @@ -167,10 +167,7 @@ public static void invokeAndWaitOnEventThread(Runnable runnable) { } else { try { SwingUtilities.invokeAndWait(runnable); - } catch (InterruptedException e) { - log.error("Error invoking runnable", e); - UIUtilities.invokeOnEventThread(runnable); - } catch (InvocationTargetException e) { + } catch (InterruptedException | InvocationTargetException e) { log.error("Error invoking runnable", e); UIUtilities.invokeOnEventThread(runnable); } diff --git a/src/main/java/org/broad/igv/util/ChromosomeColors.java b/src/main/java/org/broad/igv/util/ChromosomeColors.java index 74d9b3952f..96711195c5 100644 --- a/src/main/java/org/broad/igv/util/ChromosomeColors.java +++ b/src/main/java/org/broad/igv/util/ChromosomeColors.java @@ -55,7 +55,7 @@ public static synchronized Color getColor(String chr) { private static void initColorMap() { - colorMap = new HashMap(); + colorMap = new HashMap<>(); colorMap.put("chrX", new Color(204, 153, 0)); colorMap.put("chrY", new Color(153, 204, 0)); colorMap.put("chrUn", Color.DARK_GRAY); diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index 8ce9bd3cff..d9b8dd1df8 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -128,6 +128,7 @@ SAM.SMALL_INDEL_BP_THRESHOLD Indel size threshold (bases) integer 0 --- SAM.FLAG_CLIPPING Flag clipping > threshold boolean FALSE SAM.CLIPPING_THRESHOLD Flag clipping threshold (bases) integer 0 +SAM.SHOW_CONNECTED_CHR_NAME Show connected chromosome name boolean TRUE --- SAM.QUICK_CONSENSUS_MODE Quick consensus mode boolean FALSE SAM.SHOW_INSERTION_MARKERS Show insertion markers boolean FALSE diff --git a/src/test/java/org/broad/igv/sam/BisulfiteBaseInfoTest.java b/src/test/java/org/broad/igv/sam/BisulfiteBaseInfoTest.java index c8952ab895..a0cd9636e0 100644 --- a/src/test/java/org/broad/igv/sam/BisulfiteBaseInfoTest.java +++ b/src/test/java/org/broad/igv/sam/BisulfiteBaseInfoTest.java @@ -86,11 +86,6 @@ public int getMappingQuality() { return 0; } - @Override - public ReadMate getMate() { - return null; - } - @Override public Strand getReadStrand() { return null; @@ -101,11 +96,6 @@ public int getInferredInsertSize() { return 0; } - @Override - public String getCigarString() { - return null; //To change body of implemented methods use File | Settings | File Templates. - } - @Override public List getGaps() { return null; @@ -160,11 +150,6 @@ public String getSample() { return null; //To change body of implemented methods use File | Settings | File Templates. } - @Override - public Object getAttribute(String key) { - return null; - } - @Override public void setMateSequence(String sequence) { @@ -334,10 +319,5 @@ public String getLibrary() { public String getClipboardString(double location, int mouseX) { return null; } - - @Override - public void finish() { - - } } } diff --git a/src/test/java/org/broad/igv/sam/ClippingCountsTest.java b/src/test/java/org/broad/igv/sam/ClippingCountsTest.java new file mode 100644 index 0000000000..a70305aa32 --- /dev/null +++ b/src/test/java/org/broad/igv/sam/ClippingCountsTest.java @@ -0,0 +1,53 @@ +package org.broad.igv.sam; + +import htsjdk.samtools.TextCigarCodec; +import org.junit.Assert; +import org.junit.Test; + +import java.util.List; + +public class ClippingCountsTest { + + @Test + public void testFactories() { + List booleans = List.of(true, false); + for (boolean lh : booleans) { + for (boolean ls : booleans) { + for (boolean rs : booleans) { + for (boolean rh : booleans) { + StringBuilder cigar = new StringBuilder(); + cigar.append(lh ? "1H" : ""); + cigar.append(ls ? "2S" : ""); + cigar.append("5M"); + cigar.append(rs ? "3S" : ""); + cigar.append(rh ? "4H" : ""); + ClippingCounts expected = new ClippingCounts(lh ? 1 : 0, ls ? 2 : 0, rs ? 3 : 0, rh ? 4 : 0); + Assert.assertEquals("from string", expected, ClippingCounts.fromCigarString(cigar.toString())); + Assert.assertEquals("from cigar", expected, ClippingCounts.fromCigar(TextCigarCodec.decode(cigar.toString()))); + } + } + } + } + } + + @Test + public void testForWiringIssues(){ + final ClippingCounts counts = new ClippingCounts(1, 2, 3, 4); + Assert.assertEquals(1, counts.getLeftHard()); + Assert.assertEquals(2, counts.getLeftSoft()); + Assert.assertEquals(3, counts.getRightSoft()); + Assert.assertEquals(4, counts.getRightHard()); + Assert.assertTrue(counts.isClipped()); + Assert.assertTrue(counts.isLeftClipped()); + Assert.assertTrue(counts.isRightClipped()); + Assert.assertEquals(3, counts.getLeft()); + Assert.assertEquals(7, counts.getRight()); + + final ClippingCounts empty = new ClippingCounts(0, 0, 0 ,0); + Assert.assertFalse(empty.isClipped()); + Assert.assertFalse(empty.isRightClipped()); + Assert.assertFalse(empty.isLeftClipped()); + Assert.assertEquals(0,empty.getLeft()); + Assert.assertEquals(0,empty.getRight()); + } +} \ No newline at end of file From 8d1f8909dce7e05425faa01b80cfff31644e7a35 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 1 Jun 2023 14:38:58 -0700 Subject: [PATCH 056/116] Add batch command "scrollToTop". See #1336 --- .../org/broad/igv/batch/CommandExecutor.java | 3 ++ src/main/java/org/broad/igv/ui/IGV.java | 24 +++++++++- .../igv/ui/panel/TrackPanelComponent.java | 11 +++-- test/batch/many_tracks.txt | 45 +++++++++++++++++++ 4 files changed, 77 insertions(+), 6 deletions(-) create mode 100644 test/batch/many_tracks.txt diff --git a/src/main/java/org/broad/igv/batch/CommandExecutor.java b/src/main/java/org/broad/igv/batch/CommandExecutor.java index eaae030f07..d941715fdc 100755 --- a/src/main/java/org/broad/igv/batch/CommandExecutor.java +++ b/src/main/java/org/broad/igv/batch/CommandExecutor.java @@ -121,6 +121,9 @@ public String execute(String commandLine) { } else if (cmd.equalsIgnoreCase("scrolltotrack") || cmd.equalsIgnoreCase("gototrack")) { boolean res = this.igv.scrollToTrack(StringUtils.stripQuotes(param1)); result = res ? "OK" : String.format("Error: Track %s not found", param1); + } else if (cmd.equalsIgnoreCase("scrolltotop") ) { + this.igv.scrollToTop(); + result = "OK"; } else if (cmd.equalsIgnoreCase("snapshotdirectory")) { result = setSnapshotDirectory(param1); } else if (cmd.equalsIgnoreCase("snapshot")) { diff --git a/src/main/java/org/broad/igv/ui/IGV.java b/src/main/java/org/broad/igv/ui/IGV.java index 89f0e7bcd9..dd00fd6c8d 100644 --- a/src/main/java/org/broad/igv/ui/IGV.java +++ b/src/main/java/org/broad/igv/ui/IGV.java @@ -939,13 +939,33 @@ public List getTrackPanels() { } + /** + * Scroll panel(s) containing track with specified name to the top. Supports batch command "scrolltotrack" * + * + * @param trackName + * @return + */ public boolean scrollToTrack(String trackName) { + boolean found = false; for (TrackPanel tp : getTrackPanels()) { if (tp.getScrollPane().getNamePanel().scrollTo(trackName)) { - return true; + found = true; } + } + return found; + } + + + /** + * Scroll all panels to the top (position 0). Supports batch command "scrolltotop" + * + * @return + */ + public void scrollToTop() { + for (TrackPanel tp : getTrackPanels()) { + tp.getScrollPane().getNamePanel().scrollToPosition(0); + } - return false; } diff --git a/src/main/java/org/broad/igv/ui/panel/TrackPanelComponent.java b/src/main/java/org/broad/igv/ui/panel/TrackPanelComponent.java index a482791d13..d4d250999d 100644 --- a/src/main/java/org/broad/igv/ui/panel/TrackPanelComponent.java +++ b/src/main/java/org/broad/igv/ui/panel/TrackPanelComponent.java @@ -120,15 +120,18 @@ public boolean scrollTo(String trackName) { if (t != null) { IGV.getInstance().clearSelections(); t.setSelected(true); - if (trackPanel.getScrollPane().getVerticalScrollBar().isShowing()) { - trackPanel.getScrollPane().getVerticalScrollBar().setValue(t.getY()); - } + scrollToPosition(t.getY()); return true; } - return false; } + public void scrollToPosition(int y) { + if (trackPanel.getScrollPane().getVerticalScrollBar().isShowing()) { + trackPanel.getScrollPane().getVerticalScrollBar().setValue(y); + } + } + int searchIdx = 0; private synchronized Track findNextTrackMatching(String trackName) { diff --git a/test/batch/many_tracks.txt b/test/batch/many_tracks.txt new file mode 100644 index 0000000000..90825c3362 --- /dev/null +++ b/test/batch/many_tracks.txt @@ -0,0 +1,45 @@ +new +maxPanelHeight: 10000 +goto chr1:637-9,175 +load ../data/gff/canonical.eden.unsorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +load ../data/gff/canonical.eden.sorted.gff3 +scrollToTop +snapshot From 08b068262b77eb54732535d9195f36ba0cee46d1 Mon Sep 17 00:00:00 2001 From: Chris Saunders Date: Sat, 17 Jun 2023 14:59:36 -0700 Subject: [PATCH 057/116] Add a dedicated 6mA color mode (#1342) * Add a dedicated 6mA color mode This allows a user to see coloration for only 6mA modifications by filtering out any other basemod types. This can be useful when viewing a bam file containing multiple modification types, such as 6mA and 5mC. * Consolidate BaseModificationRenderer.draw Consolidate general and 6mA cases down to one method * Consolidate BaseModificationCoverageRenderer.draw Consolidate general and 6mA cases down to one method --- .../org/broad/igv/sam/AlignmentRenderer.java | 1 + .../java/org/broad/igv/sam/AlignmentTrack.java | 3 ++- .../org/broad/igv/sam/AlignmentTrackMenu.java | 1 + .../mods/BaseModificationCoverageRenderer.java | 16 ++++++++++++---- .../igv/sam/mods/BaseModificationRenderer.java | 14 ++++++++++---- 5 files changed, 26 insertions(+), 9 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java index a062b45987..10ccaab96e 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java +++ b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java @@ -1301,6 +1301,7 @@ private Color getAlignmentColor(Alignment alignment, AlignmentTrack track) { case BASE_MODIFICATION: case BASE_MODIFICATION_5MC: case BASE_MODIFICATION_C: + case BASE_MODIFICATION_6MA: case SMRT_SUBREAD_IPD: case SMRT_SUBREAD_PW: case SMRT_CCS_FWD_IPD: diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrack.java b/src/main/java/org/broad/igv/sam/AlignmentTrack.java index c8d6226df6..93b3d57c7c 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrack.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrack.java @@ -103,6 +103,7 @@ public enum ColorOption { BASE_MODIFICATION, BASE_MODIFICATION_5MC, BASE_MODIFICATION_C, + BASE_MODIFICATION_6MA, SMRT_SUBREAD_IPD, SMRT_SUBREAD_PW, SMRT_CCS_FWD_IPD, @@ -111,7 +112,7 @@ public enum ColorOption { SMRT_CCS_REV_PW; public boolean isBaseMod() { - return this == BASE_MODIFICATION || this == BASE_MODIFICATION_5MC || this == BASE_MODIFICATION_C; + return this == BASE_MODIFICATION || this == BASE_MODIFICATION_5MC || this == BASE_MODIFICATION_C || this == BASE_MODIFICATION_6MA; } public boolean isSMRTKinetics() { diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index d672926a5f..79c0b16609 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -636,6 +636,7 @@ void addColorByMenuItem() { mappings.put("base modification", AlignmentTrack.ColorOption.BASE_MODIFICATION); mappings.put("base modification (5mC)", AlignmentTrack.ColorOption.BASE_MODIFICATION_5MC); mappings.put("base modification (all C)", AlignmentTrack.ColorOption.BASE_MODIFICATION_C); + mappings.put("base modification (6mA)", AlignmentTrack.ColorOption.BASE_MODIFICATION_6MA); colorMenu.addSeparator(); for (Map.Entry el : mappings.entrySet()) { JRadioButtonMenuItem mi = getColorMenuItem(el.getKey(), el.getValue()); diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java index 69402245bd..f52eef3587 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java @@ -31,8 +31,11 @@ public static void drawModifications(RenderContext context, case BASE_MODIFICATION_C: draw5MC(context, pX, pBottom, dX, barHeight, pos, alignmentCounts, true); break; + case BASE_MODIFICATION_6MA: + draw(context, pX, pBottom, dX, barHeight, pos, alignmentCounts, true); + break; default: - draw(context, pX, pBottom, dX, barHeight, pos, alignmentCounts); + draw(context, pX, pBottom, dX, barHeight, pos, alignmentCounts, false); } } @@ -43,7 +46,8 @@ private static void draw(RenderContext context, int dX, int barHeight, int pos, - AlignmentCounts alignmentCounts) { + AlignmentCounts alignmentCounts, + boolean onlyDraw6mA) { BaseModificationCounts modificationCounts = alignmentCounts.getModifiedBaseCounts(); @@ -53,6 +57,12 @@ private static void draw(RenderContext context, for (BaseModificationCounts.Key key : modificationCounts.getAllModifications()) { + String modification = key.getModification(); + if (onlyDraw6mA) { + if (key.getCanonicalBase() != 'A' && key.getCanonicalBase() != 'T') continue; + if (!modification.equals("a")) continue; + } + // The number of modification calls, some of which might have likelihood of zero int modificationCount = modificationCounts.getCount(pos, key); @@ -60,8 +70,6 @@ private static void draw(RenderContext context, byte base = (byte) key.getBase(); byte complement = SequenceUtil.complement(base); - char modStrand = key.getStrand(); - String modification = key.getModification(); // Count of bases at this location that could potentially be modified, accounting for strand int baseCount = alignmentCounts.getPosCount(pos, base) + alignmentCounts.getNegCount(pos, complement); diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java index aa64abe903..50ba94ebfe 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java @@ -24,9 +24,11 @@ public static void drawModifications( case BASE_MODIFICATION_C: draw5mC(alignment, bpStart, locScale, rowRect, g, true); break; - + case BASE_MODIFICATION_6MA: + draw(alignment, bpStart, locScale, rowRect, g, true); + break; default: - draw(alignment, bpStart, locScale, rowRect, g); + draw(alignment, bpStart, locScale, rowRect, g, false); } } @@ -45,7 +47,8 @@ private static void draw( double bpStart, double locScale, Rectangle rowRect, - Graphics g) { + Graphics g, + boolean onlyDraw6mA) { List baseModificationSets = alignment.getBaseModificationSets(); @@ -73,6 +76,10 @@ private static void draw( byte lh = 0; String modification = null; for (BaseModificationSet bmSet : baseModificationSets) { + if (onlyDraw6mA) { + if (bmSet.getCanonicalBase() != 'A' && bmSet.getCanonicalBase() != 'T') continue; + if (! bmSet.getModification().equals("a")) continue; + } if (bmSet.containsPosition(i)) { if (modification == null || Byte.toUnsignedInt(bmSet.getLikelihoods().get(i)) > Byte.toUnsignedInt(lh)) { modification = bmSet.getModification(); @@ -185,5 +192,4 @@ private static void draw5mC( } } } - } From 4877b30cc6a12a8a1a4e34150f5a304f94beaca5 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 15 Jun 2023 21:41:47 -0700 Subject: [PATCH 058/116] Warn on attempt to save illegal value of numeric preference. --- .../java/org/broad/igv/prefs/PreferencesEditor.java | 2 ++ src/main/java/org/broad/igv/track/AbstractTrack.java | 10 +++++++++- src/main/resources/org/broad/igv/prefs/preferences.tab | 2 +- 3 files changed, 12 insertions(+), 2 deletions(-) diff --git a/src/main/java/org/broad/igv/prefs/PreferencesEditor.java b/src/main/java/org/broad/igv/prefs/PreferencesEditor.java index d646476a16..9d5b23eb56 100644 --- a/src/main/java/org/broad/igv/prefs/PreferencesEditor.java +++ b/src/main/java/org/broad/igv/prefs/PreferencesEditor.java @@ -235,6 +235,7 @@ private static void init(final JDialog parent, JPanel panel, List Date: Sat, 17 Jun 2023 13:32:39 -0700 Subject: [PATCH 059/116] update bm test --- .../java/org/broad/igv/sam/mods/BaseModificationsTest.java | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/src/test/java/org/broad/igv/sam/mods/BaseModificationsTest.java b/src/test/java/org/broad/igv/sam/mods/BaseModificationsTest.java index e6639944b6..15efd23b9e 100644 --- a/src/test/java/org/broad/igv/sam/mods/BaseModificationsTest.java +++ b/src/test/java/org/broad/igv/sam/mods/BaseModificationsTest.java @@ -399,5 +399,9 @@ public void testHiFINegativeAlignment() { assertTrue(expectedPositions.contains(pos)); assertEquals('G', seq.charAt(pos)); } + + for(int i=0; i<=255; i++) { + System.out.println(i + " " + ((byte) i) + " " + Byte.toUnsignedInt((byte) i)); + } } } From cbd6bdec7b613ef4f0d070d4f6e7c6bd2ddba184 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Sat, 17 Jun 2023 17:04:45 -0700 Subject: [PATCH 060/116] Add color for 6ma modifications. See PR #1342 --- .../igv/sam/mods/BaseModificationColors.java | 29 ++++++++----------- 1 file changed, 12 insertions(+), 17 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationColors.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationColors.java index 2dce742672..ea2ddecc47 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationColors.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationColors.java @@ -23,32 +23,25 @@ public class BaseModificationColors { private static Logger log = LogManager.getLogger(BaseModificationColors.class); - - static Color mColor = Color.red; - static Color hColor = new Color(11, 132, 165); - static Color oColor = new Color(111, 78, 129); - static Color fColor = new Color(246, 200, 95); - static Color cColor = new Color(157, 216, 102); - static Color gColor = new Color(255, 160, 86); - static Color eColor = new Color(141, 221, 208); - static Color bColor = new Color(202, 71, 47); static Color genericColor = new Color(132, 178, 158); public static Color noModColor5MC = Color.blue; + public static Color color6ma = new Color(51, 0, 111); static HashMap colors = new HashMap<>(); // 5MC overrides -- avoid colors close to blue for "C" modificatoins static HashMap colors5MC = new HashMap<>(); static { - colors.put("m", mColor); - colors.put("h", hColor); - colors.put("o", oColor); - colors.put("f", fColor); - colors.put("c", cColor); - colors.put("g", gColor); - colors.put("e", eColor); - colors.put("b", bColor); + colors.put("m", Color.red); + colors.put("h", new Color(11, 132, 165)); + colors.put("o", new Color(111, 78, 129)); + colors.put("f", new Color(246, 200, 95)); + colors.put("c", new Color(157, 216, 102)); + colors.put("g", new Color(255, 160, 86)); + colors.put("e", new Color(141, 221, 208)); + colors.put("b", new Color(202, 71, 47)); + colors.put("a", new Color(51, 0, 111)); colors5MC.put("h", new Color(255, 0, 255)); } @@ -124,6 +117,8 @@ private static Color getBaseColor(String modification, AlignmentTrack.ColorOptio if ((colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_5MC || colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_C) && colors5MC.containsKey(modification)) { return colors5MC.get(modification); + } else if (colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_6MA) { + return color6ma; } else if (colors.containsKey(modification)) { return colors.get(modification); } else { From c8dcbe8dc6e59ac844eb289c8df864c976178603 Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Wed, 21 Jun 2023 10:48:45 -0700 Subject: [PATCH 061/116] Refactor base mod coverage -- special mode for CpG 5mC (#1344) --- .../java/org/broad/igv/sam/SAMAlignment.java | 2 +- .../igv/sam/mods/BaseModificationCounts.java | 111 +++--------------- .../BaseModificationCoverageRenderer.java | 86 ++++++++------ .../igv/sam/mods/BaseModificationKey.java | 69 +++++++++++ .../sam/mods/BaseModificationRenderer.java | 20 ++-- .../igv/sam/mods/BaseModificationSet.java | 34 ++++++ .../sam/mods/BaseModificationCountsTest.java | 2 +- .../igv/sam/mods/BaseModificationsTest.java | 40 ++++++- 8 files changed, 219 insertions(+), 145 deletions(-) create mode 100644 src/main/java/org/broad/igv/sam/mods/BaseModificationKey.java diff --git a/src/main/java/org/broad/igv/sam/SAMAlignment.java b/src/main/java/org/broad/igv/sam/SAMAlignment.java index 4e8e6cd436..4b7ff70137 100644 --- a/src/main/java/org/broad/igv/sam/SAMAlignment.java +++ b/src/main/java/org/broad/igv/sam/SAMAlignment.java @@ -703,7 +703,7 @@ public String getAlignmentValueString(double position, int mouseX, AlignmentTrac for (BaseModificationSet bmSet : baseModificationSets) { if (bmSet.containsPosition(p)) { if (modString.length() > 0) modString += "
"; - modString += BaseModificationUtils.valueString(bmSet.getModification(), bmSet.getLikelihoods().get(p)); + modString += bmSet.valueString(p); } } if (modString.length() > 0) { diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java index e5c3cdcb9f..d70c6d1964 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java @@ -1,32 +1,5 @@ -/* - * The MIT License (MIT) - * - * Copyright (c) 2007-2015 Broad Institute - * - * Permission is hereby granted, free of charge, to any person obtaining a copy - * of this software and associated documentation files (the "Software"), to deal - * in the Software without restriction, including without limitation the rights - * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell - * copies of the Software, and to permit persons to whom the Software is - * furnished to do so, subject to the following conditions: - * - * The above copyright notice and this permission notice shall be included in - * all copies or substantial portions of the Software. - * - * - * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR - * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, - * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE - * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER - * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, - * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN - * THE SOFTWARE. - */ - package org.broad.igv.sam.mods; -import htsjdk.samtools.util.SequenceUtil; -import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.sam.Alignment; import org.broad.igv.sam.AlignmentBlock; import org.broad.igv.sam.AlignmentTrack; @@ -39,27 +12,21 @@ */ public class BaseModificationCounts { - - public static Key Key5mC = new Key('C', '+', "m"); - public static Key Key5mCcomplement = new Key('G', '-', "m"); - public static Key Key5hmC = new Key('C', '+', "hm"); - public static Key Key5hmCcomplement = new Key('G', '-', "hm"); - /** * Set of all modification seen. */ - LinkedHashSet allModifications; + LinkedHashSet allModifications; /** * Map for counts for each modification (e.g. m, h, etc). Key is base+modification identifier, value is map of position -> count */ - Map> counts; + Map> counts; /** * Map for capturing modification likelihood "pileup", key is modification identifier, * value is map of position -> sum of likelihoods for modifications at that position */ - Map> likelihoodSums; + Map> likelihoodSums; public BaseModificationCounts() { allModifications = new LinkedHashSet<>(); @@ -89,7 +56,7 @@ public void incrementCounts(Alignment alignment) { for (BaseModificationSet bmset : baseModificationSets) { //String modification = bmset.getModification(); - Key key = new Key(bmset.getBase(), bmset.getStrand(), bmset.getModification()); + BaseModificationKey key = BaseModificationKey.getKey(bmset.getBase(), bmset.getStrand(), bmset.getModification()); Map likelihoods = bmset.getLikelihoods(); if (bmset.containsPosition(i)) { @@ -126,7 +93,7 @@ public void incrementCounts(Alignment alignment) { } } - public int getCount(int position, Key key) { + public int getCount(int position, BaseModificationKey key) { Map modCounts = counts.get(key); if (modCounts != null && modCounts.containsKey(position)) { return modCounts.get(position); @@ -135,7 +102,7 @@ public int getCount(int position, Key key) { } } - public int getLikelhoodSum(int position, Key key) { + public int getLikelhoodSum(int position, BaseModificationKey key) { Map modLikelihoods = likelihoodSums.get(key); if (modLikelihoods != null && modLikelihoods.containsKey(position)) { return modLikelihoods.get(position); @@ -144,21 +111,21 @@ public int getLikelhoodSum(int position, Key key) { } } - public Collection getAllModifications() { + public Set getAllModificationKeys() { return allModifications; } public String getValueString(int position, AlignmentTrack.ColorOption colorOption) { StringBuffer buffer = new StringBuffer(); - for (Map.Entry> entry : counts.entrySet()) { - String modification = entry.getKey().modification; - Map modCounts = entry.getValue(); - if (modCounts.containsKey(position)) { - final Integer count = modCounts.get(position); - int lh = (int) (((100.0f / 255) * getLikelhoodSum(position, entry.getKey())) / count); - buffer.append("Modification: " + modification + " (" + count + " @ " + lh + "%)
"); - } + for (Map.Entry> entry : counts.entrySet()) { + BaseModificationKey key = entry.getKey(); + Map modCounts = entry.getValue(); + if (modCounts.containsKey(position)) { + final Integer count = modCounts.get(position); + int lh = (int) (((100.0f / 255) * getLikelhoodSum(position, entry.getKey())) / count); + buffer.append("Modification: " + key.modification + " (" + key.base + key.strand + ", " + count + " @ " + lh + "%)
"); } + } return buffer.toString(); } @@ -166,7 +133,7 @@ public String getValueString(int position, AlignmentTrack.ColorOption colorOptio * For debugging */ public void dump() { - for (Map.Entry> entry : counts.entrySet()) { + for (Map.Entry> entry : counts.entrySet()) { String modification = entry.getKey().toString(); Map modCounts = entry.getValue(); @@ -177,51 +144,5 @@ public void dump() { } } - public static class Key { - char base; - char strand; - String modification; - - public Key(char base, char strand, String modification) { - this.base = base; - this.strand = strand; - this.modification = modification; - } - - public char getBase() { - return base; - } - - public char getCanonicalBase() { - return strand == '+' ? base : (char) SequenceUtil.complement((byte) base); - } - - public char getStrand() { - return strand; - } - - public String getModification() { - return modification; - } - - @Override - public boolean equals(Object o) { - if (this == o) return true; - if (o == null || getClass() != o.getClass()) return false; - Key key = (Key) o; - return base == key.base && strand == key.strand && modification.equals(key.modification); - } - - @Override - public int hashCode() { - return Objects.hash(base, strand, modification); - } - - @Override - public String toString() { - return "" + base + strand + modification; - } - } - } diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java index f52eef3587..c1838180be 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java @@ -1,20 +1,16 @@ package org.broad.igv.sam.mods; -import htsjdk.samtools.util.SequenceUtil; -import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.sam.AlignmentCounts; -import org.broad.igv.sam.AlignmentTrack; import org.broad.igv.sam.AlignmentTrack.ColorOption; import org.broad.igv.track.RenderContext; import java.awt.*; -import java.util.Arrays; -import java.util.Collections; -import java.util.HashMap; -import java.util.Map; +import java.util.*; public class BaseModificationCoverageRenderer { + + public static void drawModifications(RenderContext context, int pX, int pBottom, @@ -32,10 +28,10 @@ public static void drawModifications(RenderContext context, draw5MC(context, pX, pBottom, dX, barHeight, pos, alignmentCounts, true); break; case BASE_MODIFICATION_6MA: - draw(context, pX, pBottom, dX, barHeight, pos, alignmentCounts, true); + draw(context, pX, pBottom, dX, barHeight, pos, alignmentCounts, "a"); break; default: - draw(context, pX, pBottom, dX, barHeight, pos, alignmentCounts, false); + draw(context, pX, pBottom, dX, barHeight, pos, alignmentCounts, null); } } @@ -47,47 +43,69 @@ private static void draw(RenderContext context, int barHeight, int pos, AlignmentCounts alignmentCounts, - boolean onlyDraw6mA) { + String filter) { BaseModificationCounts modificationCounts = alignmentCounts.getModifiedBaseCounts(); if (modificationCounts != null) { + final BaseModificationKey mCKey = BaseModificationKey.getKey('C', '+', "m"); + final BaseModificationKey mGKey = BaseModificationKey.getKey('G', '-', "m"); + Graphics2D graphics = context.getGraphics(); - for (BaseModificationCounts.Key key : modificationCounts.getAllModifications()) { + Set allModificationKeys = modificationCounts.getAllModificationKeys(); + boolean cpgMode = allModificationKeys.contains(mCKey) && !allModificationKeys.contains(mGKey); + // Merge complementary sets (same modification, opposite strands) + Map likelihoodSums = new LinkedHashMap<>(); + for (BaseModificationKey key : allModificationKeys) { String modification = key.getModification(); - if (onlyDraw6mA) { - if (key.getCanonicalBase() != 'A' && key.getCanonicalBase() != 'T') continue; - if (!modification.equals("a")) continue; - } + if(filter != null && !filter.equals(modification)) continue; + float currentCount = likelihoodSums.containsKey(modification) ? likelihoodSums.get(modification) : 0; + likelihoodSums.put(modification, currentCount + modificationCounts.getLikelhoodSum(pos, key)); + } - // The number of modification calls, some of which might have likelihood of zero - int modificationCount = modificationCounts.getCount(pos, key); + // Color bar by likelihood weighted count + int total = alignmentCounts.getTotalCount(pos); - if (barHeight > 0 && modificationCount > 0) { + for (String modification : likelihoodSums.keySet()) { - byte base = (byte) key.getBase(); - byte complement = SequenceUtil.complement(base); + if (likelihoodSums.get(modification) > 0) { - // Count of bases at this location that could potentially be modified, accounting for strand - int baseCount = alignmentCounts.getPosCount(pos, base) + alignmentCounts.getNegCount(pos, complement); + float modFraction; + if (cpgMode & "m".equals(modification)) { + // Special mode for out-of-spec 5mC CpG convention. + // Calls are made for the CG dinucleotide and only recorded on 1 strand. We adjust the height + // of the bar to account for the missing G- calls. This is an approximation and assumes the + // distribution of calls is ~ equal on both strands. + final byte base = (byte) 'C'; //key.getCanonicalBase(); + final byte compl = (byte) 'G'; //SequenceUtil.complement(base); + final int posCount = alignmentCounts.getPosCount(pos, base); + final int negCount = alignmentCounts.getNegCount(pos, compl); - int calledBarHeight = (int) ((((float) modificationCount) / baseCount) * barHeight); - Color modColor = BaseModificationColors.getModColor(modification, (byte) 255, ColorOption.BASE_MODIFICATION); + // Is this a "C" or "G" reference site? We want to determine this from the counts data directly, this should work for all but pahtological edge cases + final int cCounts = alignmentCounts.getCount(pos, base); + final int gCounts = alignmentCounts.getCount(pos, compl); + final boolean cSite = cCounts > gCounts; + final int referenceCounts = cSite ? cCounts : gCounts; - float averageLikelihood = (float) (modificationCounts.getLikelhoodSum(pos, key)) / (modificationCount * 255); - int modHeight = (int) (averageLikelihood * calledBarHeight); - // Generic modification - float threshold = PreferencesManager.getPreferences().getAsFloat("SAM.BASEMOD_THRESHOLD"); - if (averageLikelihood > threshold && modHeight > 0) { - int baseY = pBottom - modHeight; - graphics.setColor(modColor); - graphics.fillRect(pX, baseY, dX, modHeight); - pBottom = baseY; + final int detectable = cSite ? posCount : negCount; + + modFraction = (((float) referenceCounts) / total) * (likelihoodSums.get(modification) / (detectable * 255f)); + + } else { + modFraction = likelihoodSums.get(modification) / (total * 255f); + } + int modHeight = Math.round(modFraction * barHeight); + + int baseY = pBottom - modHeight; + Color modColor = BaseModificationColors.getModColor(modification, (byte) 255, ColorOption.BASE_MODIFICATION); + graphics.setColor(modColor); + graphics.fillRect(pX, baseY, dX, modHeight); + pBottom = baseY; } } } @@ -111,7 +129,7 @@ private static void draw5MC(RenderContext context, Map likelihoodSums = new HashMap<>(); Map modCounts = new HashMap<>(); - for (BaseModificationCounts.Key key : modificationCounts.getAllModifications()) { + for (BaseModificationKey key : modificationCounts.getAllModificationKeys()) { // This coloring mode is exclusively for "C" modifications if (key.getCanonicalBase() != 'C') continue; diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationKey.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationKey.java new file mode 100644 index 0000000000..a83a3c0480 --- /dev/null +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationKey.java @@ -0,0 +1,69 @@ +package org.broad.igv.sam.mods; + +import htsjdk.samtools.util.SequenceUtil; + +import java.util.Collections; +import java.util.HashMap; +import java.util.Map; +import java.util.Objects; + +public class BaseModificationKey { + char base; + char strand; + String modification; + + static Map keyCache = Collections.synchronizedMap(new HashMap<>()); + + public static BaseModificationKey getKey(char base, char strand, String modification) { + + String name = base + strand + modification; + if (keyCache.containsKey(name)) { + return keyCache.get(name); + } else { + BaseModificationKey key = new BaseModificationKey(base, strand, modification); + keyCache.put(name, key); + return key; + } + + } + + private BaseModificationKey(char base, char strand, String modification) { + this.base = base; + this.strand = strand; + this.modification = modification; + } + + public char getBase() { + return base; + } + + public char getCanonicalBase() { + return strand == '+' ? base : (char) SequenceUtil.complement((byte) base); + } + + public char getStrand() { + return strand; + } + + public String getModification() { + return modification; + } + + @Override + public boolean equals(Object o) { + if (this == o) return true; + if (o == null || getClass() != o.getClass()) return false; + BaseModificationKey key = (BaseModificationKey) o; + return base == key.base && strand == key.strand && modification.equals(key.modification); + } + + @Override + public int hashCode() { + return Objects.hash(base, strand, modification); + } + + @Override + public String toString() { + return "" + base + strand + modification; + } +} diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java index 50ba94ebfe..d0d2651571 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java @@ -25,10 +25,10 @@ public static void drawModifications( draw5mC(alignment, bpStart, locScale, rowRect, g, true); break; case BASE_MODIFICATION_6MA: - draw(alignment, bpStart, locScale, rowRect, g, true); + draw(alignment, bpStart, locScale, rowRect, g, "a"); break; default: - draw(alignment, bpStart, locScale, rowRect, g, false); + draw(alignment, bpStart, locScale, rowRect, g, null); } } @@ -48,7 +48,7 @@ private static void draw( double locScale, Rectangle rowRect, Graphics g, - boolean onlyDraw6mA) { + String filter) { List baseModificationSets = alignment.getBaseModificationSets(); @@ -76,14 +76,12 @@ private static void draw( byte lh = 0; String modification = null; for (BaseModificationSet bmSet : baseModificationSets) { - if (onlyDraw6mA) { - if (bmSet.getCanonicalBase() != 'A' && bmSet.getCanonicalBase() != 'T') continue; - if (! bmSet.getModification().equals("a")) continue; - } - if (bmSet.containsPosition(i)) { - if (modification == null || Byte.toUnsignedInt(bmSet.getLikelihoods().get(i)) > Byte.toUnsignedInt(lh)) { - modification = bmSet.getModification(); - lh = bmSet.getLikelihoods().get(i); + if (filter == null || filter.equals(bmSet.getModification())) { + if (bmSet.containsPosition(i)) { + if (modification == null || Byte.toUnsignedInt(bmSet.getLikelihoods().get(i)) > Byte.toUnsignedInt(lh)) { + modification = bmSet.getModification(); + lh = bmSet.getLikelihoods().get(i); + } } } } diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationSet.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationSet.java index 5ad234b2ed..8be28f8028 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationSet.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationSet.java @@ -2,6 +2,7 @@ import htsjdk.samtools.util.SequenceUtil; +import java.util.HashMap; import java.util.Map; public class BaseModificationSet { @@ -43,7 +44,40 @@ public boolean containsPosition(Integer pos) { return likelihoods.containsKey(pos); } + /** + * Return a descriptive string for the modification at the given position of the read sequence* + * @param pos - position in the read sequence (left to right, as recorded in BAM record, not 5'->3') + * @return + */ + public String valueString(int pos) { + int l = (int) (100.0 * Byte.toUnsignedInt(likelihoods.get(pos)) / 255); + return "Base modification: " + + ((codeValues.containsKey(modification)) ? codeValues.get(modification) : "Uknown") + " (" + l + "%)"; + } + public boolean is5mC() { return modification.equals("m") && ((base == 'C' && strand == '+') || (base == 'G' && strand == '-')); } + + static Map codeValues; + + static { + codeValues = new HashMap<>(); + codeValues.put("m", "5mC"); + codeValues.put("h", "5hmC"); + codeValues.put("f", "5fC"); + codeValues.put("c", "5caC"); + codeValues.put("g", "5hmU"); + codeValues.put("e", "5fU"); + codeValues.put("b", "5caU"); + codeValues.put("a", "6mA"); + codeValues.put("o", "8xoG"); + codeValues.put("n", "Xao"); + codeValues.put("C", "Unknown C"); + codeValues.put("T", "Unknown T"); + codeValues.put("A", "Unknown A"); + codeValues.put("G", "Unknown G"); + codeValues.put("N", "Unknown"); + + } } diff --git a/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java b/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java index 3e0037887f..54495ae2f9 100644 --- a/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java +++ b/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java @@ -38,7 +38,7 @@ public void incrementCounts() throws IOException { int[] expectedPositions = {13846181, 13846182, 13846227, 13846228, 13846232, 13846233, 13846234}; int[] expectedCounts = {1, 1, 1, 2, 1, 1, 1 }; - BaseModificationCounts.Key key = new BaseModificationCounts.Key('C', '+', "m"); + BaseModificationKey key = BaseModificationKey.getKey('C', '+', "m"); for(int i=0; i modificationSets = BaseModificationUtils.getBaseModificationSets(mm, ml, sequence, isNegativeStrand); @@ -203,10 +206,14 @@ public void testDouble() { String mm = "C+m,1,3,0;G-m,0,2,0,4;G+o,4"; byte[] ml = {(byte) 128, (byte) 153, (byte) 179, (byte) 115, (byte) 141, (byte) 166, (byte) 192, (byte) 102}; boolean isNegativeStrand = false; + List modificationSets = BaseModificationUtils.getBaseModificationSets(mm, ml, sequence, isNegativeStrand); assertEquals(3, modificationSets.size()); + // Check positions + + for (BaseModificationSet bms : modificationSets) { String mod = bms.getModification(); if (mod.equals("m") && bms.getBase() == 'C') { @@ -400,8 +407,35 @@ public void testHiFINegativeAlignment() { assertEquals('G', seq.charAt(pos)); } - for(int i=0; i<=255; i++) { - System.out.println(i + " " + ((byte) i) + " " + Byte.toUnsignedInt((byte) i)); + for (int i = 0; i <= 255; i++) { + System.out.println(i + " " + ((byte) i) + " " + Byte.toUnsignedInt((byte) i)); + } + } + + /** + * Test a aread with both A+a and T-a calls* + */ + @Test + public void test6mAAlignment() { + + String mm = "A+a,0,0,0,14,0,0,30,30,5,10,1,41,1,1,5,0,32,0,0,0,0,30,0,21,25,29,0,52,48,2,6,32,1,35,64,0,6,99,0,0,1,36,66,0,13,0,39,1,18,22,0,0,0,5,23,7,5,2,22,1,1,0,79,21,27,0,3,7,13,9,50,95,57,7,0,1,3,0,0,0,1,2,0,0,0,0,2,25,0,5,1,15,48,0,1,0,0,0,1,0,0,0,0,3,11,15,0,0,25,0,0,3,17,0,2,1,3,0,5,0,7,1,0,1,0,0,0,0,1,3,0,0,3,14,29,2,0,20,71,61,9,28,19,7,1,29,36,58,3,0,13,4,23,91,21,0,39,1,42,115,0,16,29,40,96,0,106,2,32,11,6,0,32,0,21,2,0,0,2,70,4,20,88,0,6,2,4,0,41,0,0,1,0,0,5,1,5,17,0,3,3,0,0,0,0,1,0,1,0,20,7,28,0,26,2,0,20,5,0,0,5,6,0,0,1,1,1,0,0,0,27,3,68,0,1,0,2,13,74,0,0,6,0,0,0,0,2,50,1,17,6,0,1,21,0,10,0,0,1,0,52,8,44,4,0,0,80,30,0,10,0,0,3,0,0,0,0,24,27,0,7,103,1,0,3,0,155,3,0,9,1,17,10,40,0,20,138,33,0,15,2,76,78,0,0,47,0,0,0,1,0,3,39,0,0,2,2,23,23,0,10,30,15,31,11,0,27,8,0,5,0,2,3,33,6,1,20,30,10,0,8,4,32,0,34,1,11,0,1,4,6,21,3,0,4,38,5,0,38,0,0,40,1,0,0,22,22,13,0,9,2,0,2,2,0,1,0,0,0,0,0,0,0,23,27,17,11,42,4,0,2,0,24,11,2,14,10,11,0,0;T-a,0,0,70,0,49,0,0,0,45,4,0,1,0,8,23,7,0,2,46,88,1,3,37,32,6,72,0,2,0,0,47,1,4,60,0,0,0,69,5,16,0,0,1,1,48,11,71,1,31,8,48,0,1,1,0,2,0,29,4,0,1,0,45,2,0,4,97,23,86,64,31,0,65,0,0,0,3,3,0,5,8,13,9,8,12,8,0,0,0,0,2,1,0,2,0,0,0,0,1,0,0,0,0,0,1,0,0,0,1,1,17,9,19,0,1,0,3,1,4,0,18,3,0,10,0,1,1,0,4,6,0,1,0,0,0,0,21,37,0,0,0,0,0,0,0,182,0,33,0,68,2,1,0,5,0,36,4,5,38,32,67,0,2,0,1,38,81,10,51,80,47,5,0,24,79,26,8,5,0,3,0,4,2,0,0,1,0,22,32,1,7,126,1,0,0,0,0,27,1,8,0,3,0,29,0,11,0,35,0,1,0,0,1,2,0,27,0,1,2,4,0,0,14,34,0,11,20,30,0,69,1,0,6,0,1,0,0,0,5,5,2,0,0,16,23,4,0,3,62,25,118,0,10,0,105,0,62,24,8,0,0,59,7,7,0,13,0,15,4,0,1,0,9,3,0,55,0,0,1,0,2,0,39,3,0,0,4,13,28,40,38,2,39,0,7,33,7,1,0,7,0,4,0,54,36,0,0,0,1,41,0,0,2,0,4,8,0,1,0,2,0,3,12,9,0,7,0,24,0,1,1,0,0,0,0,0,2,1,1,0,0,3,6,9,0,1,6,0,0,0,0,0,23,0,0,3,12,0,1,0,0,3,1,11,10,0,0,5,1,4,6,14,0,0,0,6,11,1,5,3,30,12,18,7,14,0,0,0,6,17,14,6,0,0,3,1,1,1,3,6,0,43,6,0,7,2,12,0,54,79,9,1,3,4,4,0,13,2,24,1,0,1,1,0,0,99,0,0,0,0,0,2,0,1,0,0,0,1,0,1,23,1,0,0,0,7,0,0,0,0,0,0,9,15,0,8,0,1,0,0,1,2,0,1,0,0,11,20,2,0,26,8,3,1,0,3,1,31,0,4,2,5,20,0,0,2,2,3,3,0,2,7,10,0,6;"; + String seq = "AATCAGTGAGTTTTCTGGAAAGGAGTGGAAGCTAATGGGAAGCCCAGTACCCCGAGAGGAGAGAACACAACATTTCTGGCTTTGCCTATAGCTAAAGCCCGTCCCGCTGCCCCGAGATTCCTTCTGGGCTGCTCCCAGTTCTGAAGGTGCTTTCCTCTGAATACCTCCAGCTCTGACTACCTGGATTAGCCTGGCATTTAACATCTTGAGCTTTGGGTCTTTTTATGAGTGTTTCTGGTCTTCCTGCTCGATTGTATATACTCAGAGGGCAGGAACCAGGGATTATGTGCCTCTGTCCCCATCATGAATCGTAGCACAGCGCTAGGCTCAGTAAATGCTGATCAATAATGAGCACCTGATTGATTGACTCTCTCCTCAGTTGCTATGTCCACCACAGGGGACAAGATTCTTGATACTGCACTCTCTAAGGTAACAACATCTTCCTCCCCAGTTCTTGTCCCCACTCTTCTTTCCTTCCCTGAAGGGATTCACTCAGGCTCTTTCTGTCCGGCAGATTGGAGAGAAAAGCCTGTTTACCAAGGAGCTTGAACATGCCCTGGAGAAGAATGAGTAAGTAAAGATAGGAGAGTGTGGTGCCCTCCCAGTCTCTTGCTGGGACCCTAGTATGCTAGGTCTCTTGCTGGGACCCGGGGTGTCAGATAGGCTGCTGGGCTTAAACCCTCAGAGAGGCTGAAGGCAGCTCATAGGTGGGTTTTTTCAGGCTTCAGAAAAGGAGAGTGTCTGGTTCTGAGCCATCTGGCTGCCTGGACTGCAAGAATGGCTGGGGGAGGGAGGGTAGGAGGGAGAGTAGGAGGGAGAGTGAGAGGAGAGCAGTTTTCATGCTCCTGAGATCTTGAGAAGGTGTGCTTCCTGAACTGCCCTAGGCTCCACCACTGAAGTAGAGGCAGGGGTGGGTGGAGAAGGGGTGAAGGCTGGCTGCTCATACCCTTTCTCTTTGCCCCCCTCTCCCATCTCTATAGAGTGGACCTGGTTGTTCACTCCTTGAAGGACCTGCCCACTGTGCTTCCTCCTGGCTTCACCATCGGAGCCATCTGCAAGTAAGAGTCTTGCAAGTAAGGGGCTTGGGCAGGGGTAGGCATCATGTGAACCTTTGCCTTTCCCTTTGGGGCCTGACCCTCTGCTTCAGGGTTATCTCCTCTGCCCTGAGGAGTGTTGACTGGTGGCAGAAAACTCAAGAAATACCAGTGAGTTGGCAATCGAGAGAGAATAGAGGTGATCTGAACTTAAATCTCTTCCCTCATTCTGTGCCCTTCCCTCCTCCCCCAGGCGGGAAAACCCTCATGATGCTGTTGTCTTTCACCCAAAATTTGTTGGGAAGACCCTAGAAACCCTGCCAGAGAAGAGGTAAGTGGGGCCTGGATAGGCAGCTTGGTGGGATGTGCCCAGAAGATGCAGGGATGGGAGGAGGAGGAAAGGAACAGTGACTGCCTAGTGTTAAAATCTCATTGTAACTTCTCTCTGGGCAGTGTGGTGGGAACCAGCTCCCTGCGAAGAGCAGCCCAGCTGCAGAGAAAGTTCCCGCATCTGGAGTTCAGGAGTATTGTATCCTTTTAGAAGAGTGACGGATCCTTTTGGAAGAGTGACGGAGACAGCAGCCAAGGAAAAAGACAAGGTCTAGAGGGCTCTGGGAGTCCGGAGAGTGGAAGGGGCTTCCAGCAAGCAGCCCGTGGGGTCAGTGGCCTGTCTGTCTTTCCATGCACTCATCCGTCCACTCATTTACAGTCTAATGTTTTCTTAGCCCCAGACAAGTGTTCAGAGTGCAAGGCATTGGGGATAATGGTGAGCAAGATAAACATTTCCCTGCATATGTAGAGTTTACGTCTTACTTAGGGATAATGCAGTTATACTGAACTGAATAGTGACTACTTCTGGAGGGATAGGGAGTACTTCCTTTTTTTTTTTTTTTTTTTTCTGAGACGGAGTCTCGCTCTGTTGCCCAGGTTGGAGTGCAGTGGCGCAATCTAGCTCACTGCAACTTCTGCCTCCTGAGTTCAAGCAATCTTCCTGCCTCAGCCTCCTAAGTAGTTGGGATTACAGGTGCCACCACACCTGGCTAATTTTTGTATTTTTAGTAGAGACTGGGTTTCACCATGTTAGTCAGGCTGGTCTCAAACTCCTGACCTCAGGTGATCCACCAGCCTCGGCCTCCCAAAGGGCTGGGATTACAGGCTTGAGCCCCGCACCCGGTCAGTACTTCCATTTTTATATGCTACTATATTGTCTTGACTTTTACAATGAATATGTAGTACATTTCATAAAACTAAATTTAAAAATAGTATGTGCTAAGTGCTCCAATAAGTGAAGTTGGGAATTTTCTGGAAACTTCTAGTTGGAACATCTAAACACAGAAGTCTGGGGTGTCAGGGAAGGTTTCTCAGAGGTCTTGTAACCTTGGCAAGTTATTTAGCCTCCCTATGTCATTTTCCTTATCTGTAAAGTGGGGATAATAATACTACCTTCCTCACAGGGTTGTTGTGAAGATGAAATGAGCTGACATATGGAAAGTACTTTTAGAGCAGTGTCTGGCATGTAGTAAGTATGATGTAACTGTTAGCTGTTAACATTAAGCTGAGAGCTGGAAGATGACTGAAAGTCAGCCAGCTAGAGAGGGAAAGACAGACTCAGGCAGAGGGAACCGCACGAGGCCCCAGATTGCCCGACACTGTGGTCCTTAGCAACTCTCCACAGCGGGGAAACCTCAACACCCGGCTTCGGAAGCTGGACGAGCAGCAGGAGTTCAGTGCCATCATCCTGGCAACAGCTGGCCTGCAGCGCATGGGCTGGCACAACCGGGTTGGGCAGGTAGGGCCTGCCCCTATCCTCTCCCAGCTCATCTGCATCTCCTTTCTGCCTTACAGTCATCCCCAATTTAGGATTTTTAGACTTTATGATTGTGTGAAAGCGATATACGTTCAGTAGAAACTGTACTTAGTACCCATACAGCCATTCTGTTTTTTACTTTCAGTACAGTATTCATTACATGAGATATTCACTTTATTGTAAAACAGGCTTGGTGTCAGATGATTTTGTCCAACTATAATAGGCTAATCTTAAGTGTTCTGAGCACATGTAAGGTAGGCTAGGTGTATTAATGCATTTTCAGCTTGTTTTCAACTTAACAATGGGTTTATCAGGATGTAACCCTATTGTAAGTCAAGGACCATCTGTCTTCACTTCTTGACCACCCCACCTCTAACACCGTAGGCTGGGAAGATTGTGAATCAGAGGCCAGACTCTAGGCTTTCATGGAGAAAATTTACAAAAAAAAAAAAAAAGAGGCCAGACTCACACTTAGGCCTACCCAGGCTTTCTAGATGATAGGGAACTCCCATCTCACTGCCAGGTGCTTTTAGACACCCCCGTGTCCACCCTTTTGACTCCCTGTTCCGCCTCCACAGATCCTGCACCCTGAGGAATGCATGTATGCTGTGGGCCAGGTACACTTGACCAGGGAAGCCACATGGTGACATATGCCTTCCCTTTGTTCTCAACCAAGAAGCTTGTCTCACAACCTTCTGCATCTGCTTCCCCAGAATAGCATTCTCAGGGAGGGGCAGACCTTGGGATGCTACCGGTCCAAAAGGCGCTGGGGAGCAAGTAGATAGAGGTGGTCCCATGCTTTGCGCCATTGGTTGGGGAAAGATCAGGCCTGATGTCCTAGGATGTTTTTCCATCAGGGGGCCTTGGGCGTGGAAGTGCGAGCCAAGGACCAGGACATCTTGGATCTGGTGGGTGTGCTGCACGATCCCGAGACTCTGCTTCGCTGCATCGCTGAAAGGGCCTTCCTGAGGCACCTGGTAGGGCCTGTGCTCCACCTGTGGAGGGCTGGGGACTTGGAGAGCTGGGAAAGGTGGCAGGGAAGATTTCTTACATGAATGCTCTGTATACAGTGCTAACTCATTCTTGTTGAATGTTGTGTATGGATAGGACCAGGTCTGGGCCCACAGTTGCCTTTTCAGTGATGTCCTCAGGTCTGTGGTCACAGGGTGGTGTTAAGAGCCCTTGCAGCTCACAAGAACTTCTTGTTACAGGAAGGAGGCTGCAGTGTGCCAGTAGCCGTGCATACAGCTATGAAGGATGGGCAAGTAAGTGGGGGGAAATGGGCGGGAAGCCAGGGAAAGGAGGACTGTGGCATTTCTTCCTGTGCATCCCAGGTTTCTAGGTAGTCCCCTCTCAGACTGTGCTGAGGCAACTGTTTTCTTCCCCAGCTGTACCTGACTGGAGGAGTCTGGAGTCTAGACGGCTCAGATAGCATACAAGAGACCATGCAGGCTACCATCCATGTCCCTGCCCAGGTACCAAAGCTGGAGGGCGAGGGGGTAATAAACAAGAGTGCATATAATCTCTTGTTCTCACCAAATCCCACCTCCTTCCCTCATACAGCATGAAGATGGCCCTGAGGATGACCCACAGTTGGTAGGCATCACTGCTCGTAACATTCCACGAGGGCCCCAGTTGGCTGCCCAGAACTTGGGCATCAGCCTGGCCAACTTGTTGCTGAGCAAAGGAGCCAAAAACATCCTGGATGTTGCACGGCAGCTTAACGATGCCCATTAACTGGTTTGTGGGGCACAGATGCCTGGGTTGCTGCTGTCCAGTGCCTACATCCCGGGCCTCAGTGCCCCATTCTCACTGCTATCTGGGGAGTGATTACCCCGGGAGACTGAACTGCAGGGTTCAAGCCTTCCAGGGATTTGCCTCACCTTGGGGCCTTGATGACTGCCTTGCCTCCTCAGTATGTGGGGGCTTCATCTCTTTAGAGAAGTCCAAGCAACAGCCTTTGAATGTAACCAATCCTACTAATAAACCAGTTCTGAAGGTGTTGTGTGTGCGCGTGTGGAGTTGGCGGGAAGATAGGAACAAACACAAAGCCCTTTCATCCTTACCTCAGAGACTGGGACTTTTGCCCAGAGTTCTCCTGGTACGTCCTTTCTGCTTCTGCCTCAATAGTTTTCATTTCACACAGAATAAATTGTCTCCCAGGAACACCAAGAAACAGAGCCACAATCTTAAATTCCTATGGTTTGCCCCTTCAGTTAACAGTAGAGCCTGTTTATATTGCATGGCCCCTCCCACCCCTATTATCAGGAAAGTATAGAAAGTCACTAATTCTACAACTCTCTTGCAAAATGAAAACAAATGCTCCATTTAAAAAAAAAACAATCCTTTAATAAAATTAGTCCATCTAAAACTCCCCAATGCCTAAGGTTCTAGTCGTGGAAGGGTTAGCTGCAGAATTCCAGTTCAGAAGCCAACGGAGGCTCAGTCCAGACAGGGATTAACCGACTTGTGCTGGTATCTAGGTGCTTGGATTGCCGAGTTGAGTTTGCTGGAAGGGAAATGGGGCGGCGTCGGGGGGCCCGACAGGCCTGGCTGCCAGTCGGCTTCCGCGAAAACGACTCTTGCTGTCCACATAGCCAGCCGTGAAGCCAAACTGCAGGCCTCTGCCCTCCCTAAATGTCCTCCTAGGAGGCAGTCACTCGGGAGGAAGATGTGCCTGTTACCAAGTGCTTCGTCCCGGCCCCAGCGCAGACCTATGAATGAAGATGGAGGGAGAGCTGATGTGAAAGGCCTGGGTCCCGGCCGCAGAAGGGGCGCCAGACCGAAATCGGCGATCGCGTCTCTCCCACCAAAGCCTCCCGGCCAGCGGCCGCGTCTGAAAGTCCTGGGCTAAGCACCACGCCGGGGGGGTACCAGACACCCCGGCTGCAGCTGCCGAGGGCCTCACTCACCTTCAGGGCCGCCGGCCTCTCGGCCTGGACTGCCCACCTCGGCCCTGCGTCAGAGCGGAAGGCCAAGTCTTCCAGAAGGTGCTGCGGCCTCCCCGCAGCCCCTTAGCGACTCGGGATGGGGCGGCTGAGTCACGAACGCCGAGGCGGGCAGGTGCGGCGCCGCCGGCCTCCCCCCGGCAGGCTCGGGTCTTCCGCGCCACGGAGGTCCCCGAAGAGCGCCCAAGCCGCATCCGGAAGGCCCGAACCCTACCGGAGGGCGGACGGCGGACAGGGCAGGGCCCGAGGCCGACGCGGCAGGCGGTGCGGGACGGGAGCGGGGGCGGGCGGGGACTCGAGGCCGGGCGGCGAAGGCGGGCGAGGGGAGGGGAGGGGAAGGGAGCCGCGGCCCGCTTGCCCCGCAGTCTGAAGCGGCTCAGCTCTTTCCATGAGGGCGGTGGTGGCCCTTAAAAGGGCCTTTGTGGTGGCATGGGGAGGCCTGGCGGCCGGCGCGGCGCGGGCCCTCTTAGTACTCCTGGGAGGCCTGGGTGGCCTTCTTGCCGCCCGAGGGCGCCTTCGGCCCCCACGGTGGCGCTGGTCTTCTTGGGCAGCAGCACGGCCTGGATGTTGGGCAGGACGCCTCCCTGGGCGATCGTCACGCCGCCCAGCAGCTTGTTGAGCTCCTCGTCGTTGCGGATGGCCAGCTGCAGGTGGCGGGGGATGATTCGCGTCTTCTTGTTGTCGCGGGCCGCATTGCCCGCCAGCTCCAGGATCTCAGCGGTGAGGTACTCCAGCACTGCCGCCAGGTACACTGGCGCGCCGGCGCCAACGCGCTCGGCGTAGTGGCCCTTCCGCAGCAGCCGGTGTACACGGCCCACTGGGAACTGGAGGCCGGCGCGCGACGAGCGCGACTTGGCCTTGGCGCGGGCCTTGCCGCCAGTCTTGCCGCGGCCCGACATGCTAGCGAGGTAGACCGGTGAAGCACGACGGCTCAAACACTAGAACAGACGCCCGCCGCAGTGTAACTGCTGTCGCGCGCGCGCCGCGAGGCCGCCTTTATAAGCCCCCAGGACACACCTCTGCGCCCTCCTGATTGGCTCTTTTCTCCAATACCCGCCTCCGGTTGGTTGCGGTTAACCCCTTGGGTGTCGCGGCTCGGCTGAGGGAGCGCGCCCGCTGATTGGTCGAATTTGAAAACCTTTTGCCCGGGGAGAAAAGGCGAGGAGGAGGGGTAGCACGCAGACTGCCAACTATCCGCTGATGTAGCCAATCCTTGTAAAGCGCGCGAGATTTAAACACTACAATCGGAAACCAGAAGCGGAAGACCAAAGGGGTGGAGTCTGAGGCCACCTAGAGAGCGTCTGGGAAATCTGTGCCGGCGTAGGATTTTTAAAGGAAAGGGGTCAATATTATAGCTACTGCAGCCCTCCTTTCCAGGTCCAGTCACTCCCTACTCCCTTCTCCTACCTGACAAAGGGAGAATGGAAAAGTGAAGAAATTAGACCCCTTAAAAGTTTTCTTTTTTTTCTTTCTTTCTTTTTTTAATAGACCGGGTGTCACTGGCTCTGTCACCCAGGCTGGAGTGCAGTAGTGTGATCTCAGCTCACTGCAGCCTCGAACTCCTGGGTTCAAGGAATCCTCCCTGCTAAACCTCCCTCGTAGCTGGTACTACAAGTGCTCCACCACACTTGGCTAATTATTTTATTTTTGTAGAGACGGAGTCTCGTTTTGTTGCCCAGGCTGTCTTGAATTCCTGGGCTCAGGCAATCCTCCTGCCTCAGCCTCCTAAAGTGCTGGGATTACAGGCATGAGCCACTGCACCCGGTGACTCTTTGAAAGTTTGTTCCCATGCCAGGAAACAGGATAACTTCAATTTTGTGGCCCTCCATGCCACCTCTAGTATCAGGATTTAAATATTTATTCAATATGTTCTGTTTGGTCTCAGTTCTGTTCACTTCTTGTTAGACGGGGTCTGATGGGGCAGAGAGATAGGCATCCTGTTGGAGGAAGTACCCCCACCACCCCTCCCAATCTACCCAAAAATGCCCATATAGGAAATATACCTACATAAACAATAAGAAAAAGACAACACTTTATTGTCTCCATTGAGAGCATGTGGCCCCCTGCTTAGTTCTTACTAATCAGAACACTTAATTTACAATCAAATGACATGTCACCGTGGAGCCTGTGTTCCTAGCCCTGGCCCAAGTTCTATCCCAAAAACCAGTGGTTCCTTGAGAGTCAGGACTCTGAAATGTGAGTGTGGATAACTGCAAAAAAAGCTGCTGTATCCCACCCTATGAGGCTGCAAAGATGGGATGGAGAGGGAGAGAGTAGCAAGTTGCAGAAAGCCATAGGTAAAATCCAATCAGTAGTCAGCAGAGGGCAAGAAACGCCCAGGATGCCAATGATCACAGAGAAAGGAAAATGCTGAGAACAAAATCTGGAGGAGTATGAAGAGTGAGAGAGGCCTGGGCAAGGAGGCAGTCTGGGACCAGAGAGAGAGGTCAGCCTGAGTCAGGAATCCTAGAGACTGTGAGGTAAAGGACAATGATCAAGGGACTCAGACATCATAGAAGAGTCGAACGAGCTGATATCGAATGGAGAAGGTGATGGCACTGCCCAGGATCTGCAAGGAGAAATGGACTGGAGTAAGAATCACGCAGAAAGGGAGACACGGAGGTATAAACTCGATTCCCATCCTCACCTGCAGCAGCAGCAGGAGCAATGTGAGGTTTCTCTCATGTATGGGCCCAAGGACTTTAAGTAGCAAGTTGATGAGGGTCATGTTGTTACATTCAGTGAATTCACCATCTTCAGTCTCACTCTGGTGTACTGTCACCAGCTGGAGGCTCTCTGCCACCTGGAGGGACCAGAGGTGAAGAGAACCTCAGCTAATACCTATGCTTTAGGAATGTGGATCTATTTAATGCTTTCAAGTTCCCCTTGGATCTGATGTAGGACTAGGTTCCCCATTCCTCAAGGTCAGGATGGCAGCCTAGGGCCTTACCTCCTTGTTACCCTGTTACCTTTAAAATAAAGGTGCCCAAGAAAGAGAGGCTCTTGGTCTTGAACTTGGAATAGCTCATCTCCAGTTTGCCTGTCTTGATATTGAGTCTGCGGGGGAAGATAGCTTCATGTGACTGGGCCACTATTTGAACCCTCCTCCCTGTGGCTAATAGGAAGAGCATTGAATGTGGAGTCAACCTGAGATCTATTTCTGGCTCTGCTGCTTACTGTTATCAGAACCACTGTAGCAGATTATGCAAATAAATGTGCTTTGTAAGTTATAAAGGGCTACTCACATGGAAATAGCCCTTCTTTGGGCCCACTCCCTTTGCACAGCAAATGTATAGGCTGGCATTAGATATCCCAAGTGAAAAGGCTATGATGTCCATTTCAAGCCAAAAAGCGGCTACCTGGGTATGCGGTGGCGAGGGCAGGGGATGATATGCAGGAGCTGAGGCAGTGAGTAGAGGAAGTTGAACACCTGGGGCATGAAGAATAGTAGCATGGTCTTGCTGAAGTGTCCCAAGATGCCCACCACGGCAAAGGTCATGCCAGCAAAGTAACAGAAGGTATCTCCCACAAACACCCGTGATGGGTACCTGTGTGGGGGAAGAGGATCCGAGCCAGTGGCAAGAGGCATTACCAATCCCTTCTCTCACATCACACCCTATGGGCACTGGACTAGCCCTGACCCTAGTTCTGACTCAGAGGAGACTCCAGTTGTGCAGCAACTCTGCAGGATCCAAGGCCCTGAATTCATCTATTCCTGGGGCCTCCACGCACTCATCCCTAACTACTACTGGGTAGAGAGAAATTTCAGAATACGACCAACTGAAGACGCAGAAAACTCCATAGAGGTATGCGCAGGTTTAGCTTCACCACCACAAGGTGGGTTATGATAAGGGCCATCTTTGCCATGTGTTCAGGTAGTTGTCCCCTTATCCACAGGCCTACTTACCAGTTGTGGTAGAGCAATCCCAAAGTGGTGAAAAAAAGGGTATCATGAAGTAGAGGGAAAAGACATGATCATCCCGACAATCACCTTTGGAAGCAAGGAAGAAAGAAGGAAAAGTTAATACCCACTTCCTCTGCAAACCTTGGTCCTATTTTTGTCTAAGTTCTGTTTACAGCTGTTATCATCCACCTCACCCAAGTCACTTTTAAGAACTCAATACTCCTTTTATGTCACCCAAGGCAATTCCCAATATTCCACCTCTTTCGTGCAGCCTTCCAAACTAGTTACTATCTTAAATTTGGTTTAGCCCATTCTGATGTAAGTTTGGATACCAACTTTACTTCTTAGCTTTGTGACCTTGATCTTGATCATGACACATTTAACTTCTCTAAGCCTGTTTTCTCATCTGTAAAACAGGGCTGATTATAACATCTGCCACATAAAACCATTACAAGATTTAAATAAGATAGTGTTTAAAAAGTACTTAGCTTTAATAAACACTTGTTGATGTTCATCAGCCAAAGACCATCAGAAACACATCTATAGTTCAAAGCTAGGTTTAATGACTTGTTGTAAAAAGGGAGACTTCAGAAACCACACACCATTGGGAACCACGGAATGTCTCAGTAAGAGGGTGTTAGGAGGGGCTTGTAGTAGCGTTTGTTTTGGGTATTTTGGGAGAGGGTGAAAGAAAGTAGTTTTGCTCTGGACTGGGTGCTGTTAGAAAGCAGGAGTAATTCATGGCCGGGTGCGGTGACTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAAGCAGGCAGATCACCTGAGGTCAGGAGTTCGAGACCAGCCTGACCAACATGGAGAAACCCCGTCTCTACTAGAAATACAAAATTAGCTGGGCGTGGTGGCACATGCCTGTAATCCCAGCTACTAGGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAGGCGGAGGTTGCGGTGAGCCGAGATCGCACCATTGTACTCCAGCCTGGGCAAGAACAAAACTCCGTCTCAAAAAAAAAAAAAAAAGAAAAGAAAAGAAAGCAAGAGTAATTCTATGATCAAGCATTTTAATGTTTATTTAAGAAATGGTAGGAACTGGCTGGGCACAGTGGCTCAAGAGTGTAATCCCAGCACTTTGGGAGGCCTGGCAGATCACTTGAGCCCAGGAGTTCAAGACCAGCCTAGGCAACATGGTGAAACCCTGTCTCTACAAAAAATACAAAAATTTGCCAGGTGTGGTGGCACATGCCTGTAGCCCCAGCTACTTGGGAGGCTGGGGCAGGAGGATCGTTTGAGTCAGAGGATGCAGTGAGCTAAGATCGCGCCACTGCTCTCCAGCCTGCATGACAGAGGGAGACCCTGTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAGAAGTGGTAGGAACTGAGTTGTTATTGGAGAGCAGCAGCAGTCGCTCATGTTAGCCGAGAGAGGGGATCTTGGCCATTTTGTGGGTTGCTCAGTGTTCTTGTGGTTTTGCCTGTGCTCAAACATGATTACAGAGGGAGGGGTCTAGTTTTAGTCTTGCTCCACTCACGGTCACAGAATGACCCTGTGTAACGTTGGGGTTCTGTGAAACTGTTTATATTCAATGGGAGAAGATCATGGCCTAGCTATTAGTGCCAATCCAGCTATAAATGACAGGGCTACTATTTTTTCTTCTCACTTTTAATAAATCTGCATTGTTATTTGCAAAGTAAGTAGCTTCCCTTATCTTCTATAATTACTACGCATATTGCTTTTTTCCCCTGAATTTCCCAAAGTCATATATCAGGCCACACCTTTAACACTCAGACTTCTCTCTCCCCACCCCCAATCCCACCTACCTTCCAACTCTACCAGGTTGAAGACAATGATGGAAGCAGAAATGACTAGTGACTGGCCAGCCTCTAGGCCGTTAATTCCTGCTAGGATATTGATGGCATTGGTACAGAACACTGCCAGCAGCCCCATGTAGACATAGTACAGGATTCCTGCGGGGAGAGATGGTAGGAAAAGAAGTAGTGCCACCTAGGGGAGGAGGATTTAAGAATTTTTAGAAAAGTGGTGAGGACGGACGATAGGATGAAGGGCTACCAGAAAGGATCTGGGAAGACACAGAGACAAAAAAGGAACACTTGGAGGAGAATGTGGAGCACCAGGAAGGGTTCCCTGAAGTAGGTGCCATAGGGGCAGCAGTGGTAGGACTACCTACCCAAGTCCAGATGCAGGCCAAGTATCGGGCGGAAGGGCTTGGGCACCACAATGGTCGTGTTGCCAAAGTTGGTGAAATAGACCATGAGGAGAGGTAGTGAGGCAGCTGTAGGTAGCAGCAGCTTATGGCGCCAGCGCAGATTCAGTACATCATCCGCAAAGCCCAGGAAGATCATGCAGCAGATGGCAAGGAGGGCACCTATCAGGGCCACAAACTGGGGGAGGCTCGGGCAGGTCCATGACTCAAGCCTGCTCCCATTCCACCTTTTAAGAATTCCTGTCTTTTCTGATAATTCTTAGCCCCTCCCCACAAGCCCAAATAACCCCAGTTCTCTCAGGTACCTCCCAGGTCCCAGCCACAGGCAATCACCCCCACGAACCCACTTACTTCATGGTGGGGGAATGCCTTACACTGCTCCTTCACAAAGCAGTTCAGGAAGGGGAAAGGGATGAAGCAGAAGAGGATGATAAGGAAAACAGCACCGCTGATCACTCCCTGGGATTCTGGGCTGTGGCCCAGCAGCAAGGGGGCGAGGGGGAAGAGGAAAGGGGGCGTGGTCACTCACTAGTGCAGGTGTTACAGTAACCCAAAGTGGTCTTCTCATTCCCGGTTTTTTATTCTGGTAAGTGGTGAGGGGGGCGGAGGGAGGAAAGCACCACTGCCAGAGTCCCAGATATACCCAAGGGTCCCTACCCATCCTTTCCCCAATGCGAGTAAGTTCTGCTCATCACCTCTCCGAGTTCCAGGCTGCCTGGGTTAGTTCTGAGTCTTCACGTGTGGTCAAGCACCCCGGTTCCCGCCCCTGCCCTAGCCACTCCTTCCTTAGCCCTTGCCCCCTGCCCGGACCCGTGTGCCGCTGCTCACATCTGCTGTCGGCTGGTTTTGTTGAGGTCCTGACCACAGAGGCGCGCAGCAATGAAGTGGCCCCGGAAGGCCGGGATGAGGGTGACTGTGGCCACAAATCCCAGCAGCGAGACGATCAAATTGATCAGCAGCGGCATGGGCAATTCCGAGAAGGCCCACATGGTGACCGGTCAGGGGCCCGGCTCCGCCGCCTCTTCAGGTAACGGGCAAGCTGAGCAGCAGTCCTGAGGCCTCAGCAGTATGGAGTGGCCGCTCCCCACAGGCAGGCTCTTCCCACACCAATCTGAGCAAAACCCAGCAACTCTGACTTGAGCCGCCGTCGGGACACCGGGGAACCTCTCTAAGGCAACCTATGTTCTGCCCCGCTGCACCCGCCTATCTACTGTTTCCGCCCGGATCTTGTTCGCTGAACAACCATTACTGCGGAAGGCGGCGGCCGCCATTTTTAATATGGGCATTAGAGGGTGGGGATGGAAGTGGAAGAGAAGTGTGATTCCAAATTTCATCCTATGTTCAAGAGTAGAATCACCGAGAGAGTACTGGAAACGCAATTAAAGAAAGGCAGCCAAGAACGGATACTTCCCTAACATTATGTTAAAGATGTACTACCCAGAGCGGAAATGCCAATTGCCCGGTTCCAAGATGAATGCCTCCTTGTACCACGTGTCTGACACCGCTCACATTCGGTTCCACGTCAGTTTTAGCTCCGTCCGCCTCCATAGGTCAAGCTTAAAGGGCCCGTACCTCTCCCTTAATCTGGGATGCCTCCGGCTCCAGGAAGATGGCTATTGAAAGGCAGGGGGTGGGGAGGAGGACGAGGGTGTGGAGACGCGCCTGTTTCTTGCAGGAAACCTGTTGCCAACATAGTCGGAAGCCAAGGTAAACACAACTCAGTTCCCGGAAACGCAGCTACGATTTGGAAGGATAGTGTAAATTCTAAAGCGTAGATCCTTGAAAAGCCCCTCCCACTCTGTTTTTCTTGTCCTCGATTCTGGTATTTTTTTCTCAGCATCCCACCCACCTTCTGCCCCAGCTGTAGCAGTTAGTTGCCCGGCAACCCAGCGCAGCACTTCACTGAGGGATTGGGAGTACCCCTCAGGTCTTGGATTTAACTCGAGACTTCAGCTTGGCCGTGGATCACTGCATTGTACCTAGCTCCCACCAAGGAGGAGATGGAGATTTCCAGGCTGAGGCTCTGGAAAGAGCTGAGGAGGGAGAGGAATCAGACCCACAACTGATTTCCTGTGAGTCTGAGGAACTCCCAGTCAGGTTTCTTCCTGTAGGTCACTGTGCATTTTGCTTGGCCTCCTGCAGGTTGCTGTGCATTTTGCTTGGCCTGTTCCCCTGCAAACAACAATTCACCAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGACAGAGTTTCGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCGCGATCTCGGCTCACCGAAACCTCCACCTCCCAGGTTCAAGAGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCAGTAGCTGGAATTACAGGCATGCGCCACCACGCCCGGCTAATTTTGTATTTTTAGTAGAGACGGGATTTCCCTATGTTGGTCAGGCTGGTTTGGGACTCCCGACCTCAGGTGATCTGCCCGCCTTGGCCTCCCAAAATGCTGGGAGTACAGGCGTGAGCCACCACGCCCAGCCAGTTCACCAGCATCTTTATTACCTTTCATTCCCCGACAGGCTCTTAGGCGGCAAATGATTTCCATTTGTCAGAGAGGAAGTCACCACCAGTGAGTTAGGGAAAGGACTCCAATAGCCTAGCGCTTTGTCCACTCTTCCCTCTATGGCATGTTCCAGGAGTCAGGGTCATGTTGACCGTTTTTAAGCCGAAGATAGTTTCTTCTTTCCCTCAAAATCAGATGTGAGGCCGGGCGCAGTGGCTAACGTCTGTAATCCCAGCACTTTGGTAGGCGAAGGTGGGTGGCTCACTTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACACGGTGAAACCCCGTCTCTACTGAAAATACAAAAATTAGCCGGGTGTGGTGGCACATGCCTGTAATCTCAGCTACTCAGGAGGCTGAGGCAGGAGGATCGCTTGAACCTGGGATGAGGAGGTTGCAGTGAGCCGCGATCATGCCACTGCATCCCAGCCTGGGCAACAGAGGGAAACTCTGTCTCAAAACAAACAAACAAACAAACTAAATCAGATGTGAAATGGCTTTTCTACTTGAAAATGGCTCTAATTAACAACAACAAAAACCCAGATATGTATAAAATACATAAATTTTCGTAATTTACTTGCTAGTATTCCACCAGATTTTCAGTACTTATCCCACTGCTATTATTTTTCTGTGTGTGTTGTGTGTATGCACGTGTGCCCCTTTTAAACTAGAAACTCCCGGGAGGCAAGTGTTTTCTCTTCCACAGGGTGTCCCCATGGGGCTAGGAACACAGACATCTGTGTACAAGGAAGGCATCATCACACATAATGAGGCTGAGGCTGAAAGGAGGACTGAGGGCAATAGGTGCATATGGAGGGAGCAATTAGTTGTGACCAGAGTAAGTGGTCTTGAGGAAGAGAAGTATCATTTCAGTAGATTATAAGAGTAGAAGGACCTTTCACAGGCAGCGACAGTGAGACGCCAGAAGCAGGTGGAAAGGAGGAGCTTGTGGGATCCCAGAGGAGGCAGCATTCAGTTATCTTGTTGATGACGAGTATACTGAGACTCCCTGGAGGTCTGAGAGCCAGTCTGTATCTATTTCCTTTTTTATTAGGAGGCCAATCCTAAATAAATCTTCCTTCCCAGCCTGGTATTGGGAAGCTTTATCTCTTTCTTCTCCAGAGATCATTAGCCCTCCCTACCACCACCCTCCTGGGGAGGTGGGAGGATGTAATCAAAGACAAGAGAGACTAAGGATTCATCATCCACATATACATGTTACTATCAGAAAATGGTTACTAAGATACTTGTCAGATAATCTGAGTATTTTTGTTCCCATGATGATAAAGTCCCAGTTGATGATCAGACTAATCAGAAGGAGAAACAAACACATGACTTTTGGCTTCACTTGAAATTGTGTGCCTTCCCTGGTAAAAGTTCAAAGCGTTTTCCTGAATGCTGAAGCCGGCCCTAGGATTCACAGACCAGTTGACTTGTCAGTGGCCCTGGACTGAGCAGACAGTGTTTGAGATGCACATCTCTTCTAAGTAGCCTTGAGTGGCTCCCAATGAGCAAGGCCAATCCACTGAATGTGATTGCTAGACAAAGCCTGTCCCAAAAATGTTTCATAGTGCCACAAAGACAATGAGACCCCTCTGGATAGTCTGGGCTGTGATACTTGCAGGACTCTATCTGTGGCTCTGTCCAGTCAGCTGAATCACTCTTTTTCCTTTTCCTCCCACCTCTCCCCTCTTAGTCTAGAAAAAAGTTGTTAATTAAAAGCAAGCCCCAGGATTTCCCATTCCACTGGTATGGCATGAATGATATTTCAAAAGACAGAAATGGCATAGAAATTCTCAAAGCATACTTGAGTATTTTCTGTTAGACTTCTTAAAATTACAAAGTTTGTTAATCCCTACTATGTGCATAGGAAGCTCTTCAGTACTGTGGAGGATACAAACTAATATAATTGCAGTTGCTTGCCCCCAGGGAGTCTACTTCAGCATATTTGGAGCATCTTGGTTGAATTTGAGGGAAGAGAGATGACTAAGACATGGTTCCCACCCCTAGGCAGCTTCCTGATGCATGGAAAAAGCAGACATATAAACAATCAGCTAGACTCTAAGGCAGAATGTAAGTGCTAGAGGTCTAAGTAATGGAAGAAAGAAGGAGGACTAGGCTGGGCACAGTGGCTCTCACCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGAGGATCACCTGAGGTCAGGAGTTCGAGATCAGCCTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAATAACAAAAATTAGCCAGGCATGATGGCGCATGCCTATAGTCCCAACTACTTGGTAGGCTGAGGCAGGAGAATCACTCAAACCTGGGAGGTGGAGGTTGCAGTGAGCCAAGATCTCGCTACTGCACCCCAGCCTGGGCAACAGTGACTTTCTGTCTCAAGAAAAGAGAAAAAAAAAAAAAAAAGAGGGAGGACTAACTAGGGAGACTCATGTAACTAGGTAGACTCACAGTTCAGATACTGAATTGTGAAGCAGTATCTGGCAGCATAGGACCAAGCACTCAGTGGAGAAGTTGAGGGAATAAATCAAAGAAGTCAGAGAATGGAGATCACTAGGGGGTCTGAGGGTTCCTGAAGGATTCTTGGGGGAAGAGGGTCTTCATAGGGAAAGCTTCTACCTGGACAACTTCCTGCATAACCTGCTCCCCTAAACCCTAGCTGCTCTTCCCACTGACAGCTTCCTCCTGCATCCCTGGTTCTGGCCCTGAGTTCTCTCCTCCCTTGATTTCATGTCTTCTCCTCCCTTTGAGATTAGAAACCCAGACTCTAGGAATCTCCACTGATTTGGCAGTCCAGTGACAATGCATGGGCAGGGCCACAGGAGGTTACCTCATTCACCCCCACCTCTCCCCATCATTCCTTACAGGGAAATCTAGGGAGGCCCCAGGCTACCCCACCACCTCCTCTGGCTCAGACAAACCTGTTGCTGACCTGTCAGTTTCTGTCTAGCCCTGCCATCCCACACTTCAGAAGGCATTGTTTTATTCTCTTATTCTCATGTGCTCATTCCTCAGACAACTACTTGGGTTTGGGAGAAAGATACTTAACACCTTTCCATCCTACCTCTTTAAGAAAGGGACTGTGTGCCAAAGACCTTCCCACATGTATGAAGAAGGGGCTCTTGGGAGAATGTGAATTCCCCCAGGATTCCTTTGAATCCTTTTGCAGGTATTTCCTTCCCCTGTCCTCAGCAAGCATTGATGAGCCCCAGTCCTGTAGAGGTTGAATCAACTCCTTGAGTCTAGGAAAAGATGGCTTCTGAGGCCAGAGGTTGGGCAGTGTACCTACAGCACCATTTCTTGGGAGCTTGTTGGGATATCACATACTTAGAGTGGCAAGGTCTTGTGGCTGAATCTATTGATAGTCCAGTGCTACATTGCCAGCTCCCTTGTCATTACCACCCCCTCTACACGTGCTCCATAGGCAGGAGCTGTTACTATGGAAATACATTATTTTTAAAAACAGACACACAGCCTGGGCTTGGGAATCTCCAGAACAAACCTACACGAGCAAGGAGGTTAATGGGGCTGTTTCTGGGTCCAAGCTCCAAGAGGAAGGAGGGTGATGATGGTGGGGGGAATTCAAATTCGGTGTTCTACTCTCAACCTCTCCCAGCTTCAGGTTCTGCTTTCTCTCTGTTGACATCAGCTGGACCTCTGCCAGACACTTCTATCTGGGAGGGACGCTCTGCGTCTCCTTACCCTGGGCCTAGCTTCCCCCAGGAAATGGGAAGCTAGAAGTCGCTGAGTCTGGGGGTGTTGGGAGAAGGGGGCGGTGATTTCCACAGGGGTCACCTCCTTCTTAACCTCACAGGCGTCCGACCTGTGGAAATCTAACAAGCATAGGGGAAAGCAAAGGCAGCATCTTAGGTAAGTGAAGTTACTCTTGCCTTGAGCTGAGTGTGGGAGCACCGCGATTGCAAGATGGGGCGAGGTGATCAAAGATCCCGCCTATTTTCCACCCCGGAACCTCCTCCGCTCGCTGCTAGCCCCTCCCGCCCTCCTCCAGCGAGGGAGCGCCAGGCTGTCCTGGCCCCGCCCGGCGCGGGTGCGGCTCCTTTAAGGGCGGGTGGGGGCGGGGCGCCGGCGGTGTTGTCAGTGTCAGCTCTGCGCACGCAGCCCTCCCGGAGCCGGTGCCGGCCCGCGAGCCCCAGCGTCTCTGCAGACCAGTCCCCTCCAGGGTCCGGCGGGGCCCGCGCGGTGGGGAGGAGTCGGGCACTGGCCGGCGACTAACGGGTCCGACTGCTGACCAAGCTAGGAGAGACCCGGACCACGGAGACAGAGACCCCGGCATCGCGACCCCCGAGGACCTCCTCTCCTCGCCCTGTGGCACCCACTAGTCCTGGCACTCAGTCGCGGAGAGCCCCCGACCCCGCGCGCCCAGCCCCGGGGGAGCCCACCTCCTCCCCGCGGCCCGCCCGGGCCAATGCTCCCCCGGGGCAGCGGCTGAGCCCCAGCCGGGACCGGGATCGGAGCCCGCGCGGAGCATGGATCCGGGCTGGGGGCAGCGGGACGTGGGCTGGGCGGCCTTGCTGATCCTCTTCGCCGCCTCGCTGCTCACGGTGTTCGCCTGGCTGCTGCAATATGCCCGGGGCTTGTGGCTGGCGCGGGCCCGCGGGGACCGGGGCCCGGGACCCGCCTTAAGCCGGGGAACCCGCGGGTTCCCTGCGGGAGCTGGGCGTGTGGCGCTCGCTGCTGCGGCTGCGGGCGACTCGGGCTGGCGCCGCCGAGGAGCCAGGAGTCCGGGGCCTCCTGGCGTCACTCTTCGCCTTCAAGTCTTTCCGGGAGAACTGGCAGCGGGCTTGGGTGCGAGCGCTGAACGAGCAGGCCTGCAGAAACGGGGTGAGTTGGACCAAGCGCTTGGGTGGTCCCTTCAGCCTTTCCTTCCAGGGGAGGGACTGACTGCTCGTGCTAGGAGGCCAGACGAGGGTGGGCATAAAACTGGGGAACCCTATGGCTTCTCTTCCCCTCCCGGATCAGAGAAAAGGGTGCCGCTAAGGCTGGAGGTTGTAGAGATGAAATCCAACTGGGTCCCCAGACTTCCATTTCTGTGGGCAAAGTACTCTGGCTCCAAACCCATGCACAAGTTGCTGTTCTCATAGCAACGCTAATCCCTGTGCCTGAGGGCACTTCCGCCTTGCCTGGGTGCTGGTTGTGTTGGGGCATGGGAGACAGTGCACCTGCAGAAGGAATTTGCCTGCTTCTCATGCCACCACACCCATTTAACATTTCATGCCCCAAATCCCTGGTGTGGTCAGGCAGATCCCCAAGGAGCCTGAATCACCCCCTACCCGACAGTAATTTCACTGCTACAGTCAAACACATCCTTCTCCTCCATGCTGGCCTGTGGTTCACTGGGGGTTGGGGAGTGTTCAGGTGGAGAGCAGATTGACAGTGCCCCTACCAGTAGATGAGGCTTGGAACTGCCCCTTGAGGGTCAGTCACAAACAGTCCAGCCTACTTCCTCCCATTGCACCATGGGCATCTGGCCTCCCAGGAAGTGGGGCAGGAAGCGTGGTCATGAGATGGAGATAAGGTGGGAGGTAGAAGTCTCTCTTTTCTGGAGCTCCCTCCCATCCTGACCCAGCAATCTCTCCTTGCTCTCACAGTCCATCAGCTCCTTGCTCAGAGGGTTCTCTATTTCCAGCCACTATGAGTTTGGAGGCTTTACCTTACTAGTTCCCCAGATGCCGGAAACAGAAAATGTCCACAAGAAATTGCTCTATTTACTCTGTCCAGCTGGCTGGCTAAAAGCCAGATGGCATCTGTCAGTTTACACCTTGAACCCACCTCTTCATCCAACTCTCCCTGTACCTTGTCCCTCGGTCAGAAGCAACTCCCCAGGATAGCTGACTCAACTGGACATTGGCCTCCCTTGCAGAGGGTGGGGAGATTGAGGTTTCAAGCATCCATCTCCCCATCTTCTTGGCCTAGCCTTTGAATGAGACAGCTACCAGAGCAAAGGAGTTGGCTTCCCCTGGTTTGCCAAGCAAATGAACCCTATGGATTTGTTTCCTCCATCTCAAGACCCAAGGAAGAGTTGCCAGCCAATTCTGTGCCCCTTCCTCAATCTGTGTCCGCACTGCTCTCCAGGTGGGGCTGCGGTAGGTTGCTTCCATTACTGTGGACGAATTAGGGAGCAGCTCTTTTGAGATCTAAGCAGCTTTCTGCTCTCTAATACCGTCCTGCTATCTGCCTTGAGGAATTAGCCAGGCCCCTGGACTCTTGGGTCCTCTGAGCTTGTATCTCTTCCCAGTCCCCCTGGTACGGATCCCCAAGGCCATGCTCATCTTCATCTCCAAGAATATGAAGCATCATACTCTTGAAGCCTCCACATCTTATTTATTTGGAAGAAGGCTTAGCTTCATTTCTGCAGCTCTTATCTTGAACAAGGTGGCTCTACCACCTTTACCGGGGCTTAGCCCTTTCCCTCTGCCCAGCTATTATATATAGGTGGCAACCAGCACCTTAACTTTTTTCTATGTAGGATTTTTTGTTTGTTTGTTATTTGTTTTTTTAGGGGGTAGGGTAGGGGATGAAAGGATTGCAGGGAGAAGAAGAAGGAAGCTCTTGGTATTCCAGGAGGAAGCCAGGGTCTTCAGCTTAAGGTGATCTAGTCTCTGAGGTGGACTACCTCCCCATTGCTCAGTGGCTCCTAGCAGCTACAGGCCCTGGAGAGGCAGGCCTTGAGGGGACTACCTCCTGTCTTAAAGCCCTGAGCCAAGGAGGGGCCAGAAGCCAGCCTGCAGCTGAGGCCTCCGCAGTGGCAGAGTCCAGCCAGCAACTGAGCAGGCCAACCCTGTGGGGGGCAGCTCCAGAGACCTGATCCAATGCCCACATTACTCCCTCAGAGCTCCATCCAAATCGCCTTTGAGGAGGTGCCCAACTCCCACCCAGAGCCAGCATCAGTCATGTGACCTGCGTAGACCAATCTGAGCATACCATGGTAAGGGTCTGATGGGCACTGCCCTCTTTGGGGTCCAAGAAGGACTGACCCCAAGGGCTCCCTTTAGTCCAGAGGTTCTTAACCTGGAGTCTGTGAATGCCTTATGGATCTAAGGGTTGGTTTCAGGAAGTCTGAGAATCCCATTGAAGTTATGTGCAAAATGTTGGATGTGTGTTTATATGCATTTTTCTGGGGAGAGGAGTCTTTGGCATTTATCTGATTCTTTTAGGGATTTATGATCCTGAAAACATGAAGTCCTTAGGAAAACGGAAGTGGGGAGGGGTCTGCTGAACTATTACAGGCAGTTCAAAGCAGGGTGAGCACCCTTCCCCCACATCTGACCCACCTCGCCGGTCTCAGGTGCTGCGTTGCCAGCTCTCTGCTGAGGAGGTGCGGTTCCCAGTCTCTGTGACCCAGCAGTCCCCCGCTGCCGTCTCCATGGAGACCTACCACGTCACTCTGACACTGCCACCAACACAGGTAGAAGGGGATGTGGGAAACTGAGTTGGGCAGGGGGCGGTTCCATTGGCTCAGCTTCTTCCATTTGTTTCCTCTCTGGGATGGAATTCAGGAAGGGAGAGTCCTCGAATTAGGAGTCCTTGGGTAAAATGGGGCAAGTCAGCCCAGTCACTTTGTTCCTGTCTGTAGTTGGAAGTCAACCTGGAGGAAATCCCTGGTGAGGGGCTGCTCATATCCTGGGCCTTCACTGATCGCCCAGATCTCAGCCTAACGGTGCTTCCCAAGCTTCAGGCCAGGGAGGTAAGGAGGCAGAGCTGGCAGAGAAGAGGCAGAACGGGGAGGGAGGCAGAGGTGGGGGATCCACCTCCTTGTTGTTCCCTTCTTCTCTCTGCAGAGAGGTGAAGAACAAGTGGAGCTCTCCACAATTGAGGAACTGATCAAGGATGCCATAGTCAGCACCCAGCCAGCCATGATGGTCAACCTCAGGGCTTGCTCTGCCCCAGGAGGCCTGGTGAGTTGGCACCCAAAGCAAAAGATTTGCATGCACCTTGTTCCTAGGGTGCTATGTCAGGATTCTTGCTCTTTGGACCTTCTTCTGCTCTTAGGTACCAGTGAGAAGCCACCATGATGCCCCAGGCTCAGCCAGCCATCCCCAGACCTAACCGGTTATTCCTACGGCAGCTTCGGGCATCTCACTTGGGAAATGAGCTGGAAGGTGAGAGCTGGAAGTGGCTGCGGGACTGCCAAGGCTATGGTTGGTTGCAGATGCTCTCTGCCCCTCTGCCAAGGGTACAGCCTCTTTATTCTGCAGTGACTTCCCATCTCTGATCTGAGCATCTTCTAACTTCTGGTTCATCACAGGCACCGAGGAACTGTGCTGTGTAGCTGAACTCGACAACCCCATGCAGCAGAAGTGGACCAAGCCCGCGAGGGCTGGATCCGAGGTGGAGTGGACAGAAGACCTGGCACTGTAAGGAGTAACCCTGCCCCGACCCCATGCTCCAGAGCAGAGAGGATCAAACCTTTCCAGCACAGTCCTTGCAGGAATCAGCTCTGTTTTCAGTAGCCTTTGGCGTGAGCTCCTATTGTATGCCAGGCCCTGGCGTGGGTCTTAGGGATACAGACTTGACCAAGATACAGCCCCCACCCCTGGGTCAATGACAAGCTTGAGGCAGAGACAGGTGTACATAAAGAAGATGGTAAAACCGCTCCAGTTGAGGTTTATGTAATGTGCCGTCAGAGTGCCTAGGTGGGTGGGGAGATTAATTCCCATGAGGGAATAAGGATCAGAG"; + final boolean isNegativeStrand = false; + + // Set expectedPositions = new HashSet<>(Arrays.asList(23, 26, 36, 45, 70, 109, 197, 225, 240, 255, 285, 296, 312, 347, 366, 398, 436, 577, 650, 652, 717, 749, 841, 932, 983, 1005, 1011, 1073, 1195, 1253, 1411, 1451, 1472, 1497, 1503, 1540, 1594, 1605, 1734, 1743, 1748, 1767, 1780, 1789, 1868, 1910, 1929, 1947, 1982, 1989, 2018, 2039, 2051, 2063, 2068, 2080, 2092, 2113, 2141, 2155, 2164, 2183, 2191, 2196, 2221, 2223, 2250, 2422, 2430, 2486, 2624, 2653, 2751, 2780, 2801, 2855, 2934, 2977, 3003, 3030, 3056, 3105, 3107, 3130, 3163, 3225, 3246, 3308, 3396, 3399, 3477, 3514, 3532, 3544, 3589, 3633, 3651, 3681, 3702, 3737, 3763, 3796, 3800, 3802, 3833, 3836, 3904, 3907, 3943, 3977, 3997, 4007, 4036, 4046, 4063, 4087, 4115, 4125, 4130, 4190, 4194, 4257, 4261, 4350, 4371, 4423, 4446, 4455, 4521, 4640, 4652, 4667, 4707, 4732, 4735, 4795, 4834, 4853, 4899, 4924, 4941, 4960, 4980, 4995, 5007, 5016, 5040, 5061, 5086, 5095, 5097, 5106, 5139, 5145, 5148, 5161, 5189, 5199, 5209, 5229, 5236, 5282, 5328, 5353, 5400, 5422, 5459, 5465, 5517, 5524, 5529, 5558, 5573, 5585, 5587, 5592, 5611, 5614, 5655, 5674, 5697, 5704, 5707, 5711, 5717, 5734, 5751, 5788, 5790, 5845, 5866, 5888, 5899, 5914, 5916, 5920, 5933, 5937, 5950, 5967, 5969, 5974, 5976, 5981, 6004, 6015, 6024, 6027, 6029, 6032, 6050, 6071, 6082, 6099, 6102, 6106, 6130, 6132, 6135, 6141, 6148, 6153, 6157, 6159, 6165, 6168, 6194, 6200, 6208, 6225, 6228, 6235, 6239, 6242, 6252, 6259, 6261, 6278, 6283, 6285, 6291, 6319, 6322, 6344, 6357, 6363, 6379, 6386, 6388, 6390, 6394, 6396, 6401, 6405, 6419, 6421, 6423, 6425, 6450, 6459, 6462, 6473, 6480, 6488, 6498, 6501, 6507, 6512, 6533, 6535, 6537, 6539, 6547, 6552, 6560, 6566, 6570, 6574, 6585, 6596, 6605, 6609, 6612, 6619, 6631, 6636, 6642, 6646, 6657, 6661, 6668, 6676, 6682, 6687, 6693, 6699, 6706, 6711, 6717, 6721, 6730, 6745, 6751, 6756, 6760, 6765, 6771, 6775, 6778, 6781, 6784, 6795, 6799, 6824, 6826, 6846, 6850, 6853, 6856, 6859, 6862, 6868, 6883, 6886, 6892, 6894, 6898, 6904, 6966, 7007, 7020, 7029, 7045, 7053, 7060, 7063, 7071, 7090, 7116, 7128, 7161, 7171, 7173, 7193, 7228, 7236, 7248, 7253, 7272, 7274, 7283, 7288, 7306, 7312, 7352, 7359, 7364, 7371, 7380, 7386, 7397, 7407, 7419, 7426, 7455, 7463, 7474, 7480, 7482, 7487, 7491, 7497, 7517, 7519, 7521, 7523, 7548, 7551, 7555, 7582, 7588, 7597, 7606, 7610, 7625, 7629, 7639, 7644, 7672, 7676, 7681, 7688, 7709, 7713, 7723, 7729, 7739, 7744, 7753, 7763, 7770, 7772, 7820, 7822, 7829, 7868, 7878, 7900, 7938, 7943, 7950, 7972, 7975, 7980, 7984, 7990, 8002, 8011, 8029, 8031, 8040, 8048, 8072, 8091, 8093, 8100, 8117, 8146, 8154, 8169, 8185, 8189, 8194, 8233, 8253, 8321, 8338, 8345, 8349, 8352, 8358, 8360, 8366, 8382, 8386, 8396, 8402, 8425, 8435, 8439, 8444, 8462, 8469, 8471, 8483, 8490, 8509, 8517, 8523, 8549, 8551, 8555, 8566, 8569, 8579, 8591, 8594, 8624, 8627, 8632, 8645, 8648, 8667, 8673, 8697, 8708, 8721, 8752, 8757, 8763, 8780, 8794, 8799, 8805, 8839, 8843, 8848, 8855, 8859, 8865, 8885, 8907, 8915, 8926, 8940, 8948, 8958, 8967, 8972, 8981, 8983, 8988, 8990, 8993, 8996, 9006, 9016, 9020, 9047, 9050, 9076, 9096, 9100, 9140, 9144, 9166, 9174, 9184, 9188, 9194, 9224, 9249, 9295, 9303, 9327, 9419, 9426, 9443, 9478, 9480, 9490, 9500, 9506, 9508, 9511, 9521, 9554, 9559, 9561, 9570, 9585, 9593, 9653, 9668, 9672, 9689, 9695, 9708, 9710, 9736, 9745, 9749, 9754, 9758, 9784, 9789, 9796, 9802, 9808, 9814, 9820, 9828, 9844, 9848, 9866, 9881, 9920, 9925, 9933, 9949, 9961, 10083, 10277, 10407, 10458, 10474, 10538, 10560, 10568, 10621, 10627, 10665, 10713, 10731, 10739, 10760, 10938, 11111, 11152, 11336, 11365, 11406, 11417, 11422, 11597, 11634, 11858, 11938, 11974, 12071, 12204, 12222, 12282, 12297, 12337, 12353, 12383, 12400, 12407, 12431, 12452, 12458, 12462, 12474, 12504, 12515, 12535, 12593, 12603, 12605, 12610, 12748, 12966, 12982, 13049, 13055, 13058, 13068, 13076, 13091, 13119, 13127, 13242, 13250, 13305, 13322, 13339, 13364, 13376, 13409, 13417, 13422, 13453, 13464, 13469, 13497, 13509, 13513, 13574, 13645, 13648, 13680, 13688, 13722, 13754, 13786, 13814, 13824, 13952, 13996, 14026, 14039, 14371, 14377, 14403, 14409, 14430, 14445, 14460, 14472, 14479, 14496, 14504, 14520, 14546, 14552, 14566, 14575, 14583, 14599, 14614, 14636, 14646, 14684, 14698, 14705, 14727, 14731, 14751, 14771, 14774, 14783, 14785, 14795, 14820, 14833, 14850, 14856, 14880, 14901, 14938, 14942, 14967, 15065, 15128, 15200, 15213, 15232, 15238, 15246, 15261, 15280, 15299, 15357, 15365, 15396, 15403, 15452, 15521, 15549, 15559, 15573, 15584, 15592, 15616, 15629, 15631, 15639, 15643, 15650, 15687, 15692, 15727, 15769, 15856, 15879, 15886, 15893, 16045, 16091, 16137, 16160, 16207, 16229, 16235, 16278, 16292, 16321, 16326, 16359, 16372, 16382, 16384, 16403, 16417, 16425, 16443, 16458, 16464, 16469, 16471, 16491, 16495, 16512, 16567, 16588, 16601, 16625, 16628, 16646, 16666, 16682, 16690, 16709, 16730, 16761, 16787, 16806, 16815, 16833, 16837, 16842, 16852, 16859, 16864, 16866, 16870, 16888, 16894, 16908, 16930, 16940, 16968, 17095, 17159, 17220, 17339, 17366, 17374, 17518, 17708, 17807, 17817, 17839, 17860, 17928, 17956, 17958, 17970, 17995, 18001, 18021, 18147, 18238, 18377, 18428, 18537, 18547, 18555, 18577, 18591, 18681, 18704, 18738, 18740, 18750, 18761, 18797, 18800, 18815, 18821, 18964, 19249, 19281, 19287, 19308, 19314, 19332, 19347, 19375, 19400, 19409, 19416, 19455, 19460, 19468, 19579, 19583, 19587, 19633, 19878, 19964, 20007, 20012, 20209, 20241, 20288, 20305, 20307, 20326, 20332, 20373, 20393, 20406, 20464, 20496, 20532, 20560, 20601, 20630, 20638, 20656, 20688, 20730, 20783, 20798, 20840, 20925, 20929, 20947, 21005, 21143, 21147, 21153, 21235)); + + List modificationSets = BaseModificationUtils.getBaseModificationSets(mm, null, seq.getBytes(), isNegativeStrand); + assertEquals(2, modificationSets.size()); + + for(BaseModificationSet bms : modificationSets) { + char base = bms.getBase(); + Map likelihoods = bms.getLikelihoods(); + List positions = new ArrayList<>(likelihoods.keySet()); + positions.sort((o1, o2) -> o1-o2); + for(Integer pos : positions) { + assertTrue(base == seq.charAt(pos)); + } } } + } From bab4505724c987aa46f7fd5db08f497a4fe094b9 Mon Sep 17 00:00:00 2001 From: Louis Bergelson Date: Wed, 21 Jun 2023 13:49:07 -0400 Subject: [PATCH 062/116] Abbreviate Cigars more aggresively and prevent pattern recompilationo (#1339) --- src/main/java/org/broad/igv/sam/SAMAlignment.java | 10 ++++++---- 1 file changed, 6 insertions(+), 4 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/SAMAlignment.java b/src/main/java/org/broad/igv/sam/SAMAlignment.java index 4b7ff70137..3ae7d84dea 100644 --- a/src/main/java/org/broad/igv/sam/SAMAlignment.java +++ b/src/main/java/org/broad/igv/sam/SAMAlignment.java @@ -58,6 +58,9 @@ */ public class SAMAlignment implements Alignment { + public static final int MAX_CIGAR_STRING_LENGTH_TO_DISPLAY = 50; + public static final Pattern RIGHT_CIGAR_PATTERN = Pattern.compile("[A-Z](.{1," + MAX_CIGAR_STRING_LENGTH_TO_DISPLAY / 2 + "})$"); + public static final Pattern LEFT_CIGAR_PATTERN = Pattern.compile("^(.{1," + (MAX_CIGAR_STRING_LENGTH_TO_DISPLAY / 2 - 1) + "}[A-Z])"); private static final Logger log = LogManager.getLogger(SAMAlignment.class); public static final char DELETE_CHAR = '-'; @@ -772,11 +775,10 @@ public String getAlignmentValueString(double position, int mouseX, AlignmentTrac String cigarString = getCigarString(); // Abbreviate long CIGAR strings. Retain the start and end of the CIGAR, which show // clipping; trim the middle. - int maxCigarStringLength = 1000; - if (cigarString.length() > maxCigarStringLength) { + if (cigarString.length() > MAX_CIGAR_STRING_LENGTH_TO_DISPLAY) { // Match only full pairs at the beginning and end of the string. - Matcher lMatcher = Pattern.compile("^(.{1," + Integer.toString(maxCigarStringLength / 2 - 1) + "}[A-Z])").matcher(cigarString); - Matcher rMatcher = Pattern.compile("[A-Z](.{1," + Integer.toString(maxCigarStringLength / 2) + "})$").matcher(cigarString); + Matcher lMatcher = LEFT_CIGAR_PATTERN.matcher(cigarString); + Matcher rMatcher = RIGHT_CIGAR_PATTERN.matcher(cigarString); cigarString = (lMatcher.find() ? lMatcher.group(1) : "") + "..." + (rMatcher.find() ? rMatcher.group(1) : ""); } From 28a884fd916add42dc2c7dba3db31c9635fbbe74 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 21 Jun 2023 12:39:15 -0700 Subject: [PATCH 063/116] refactor 5mC coverage -- no call => low likelihood of modification --- .../BaseModificationCoverageRenderer.java | 36 +++++++++++-------- 1 file changed, 22 insertions(+), 14 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java index c1838180be..21b7667388 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java @@ -89,11 +89,8 @@ private static void draw(RenderContext context, final int gCounts = alignmentCounts.getCount(pos, compl); final boolean cSite = cCounts > gCounts; final int referenceCounts = cSite ? cCounts : gCounts; - - - final int detectable = cSite ? posCount : negCount; - - modFraction = (((float) referenceCounts) / total) * (likelihoodSums.get(modification) / (detectable * 255f)); + final int strandCount = cSite ? posCount : negCount; + modFraction = (((float) referenceCounts) / total) * (likelihoodSums.get(modification) / (strandCount * 255f)); } else { modFraction = likelihoodSums.get(modification) / (total * 255f); @@ -122,10 +119,14 @@ private static void draw5MC(RenderContext context, BaseModificationCounts modificationCounts = alignmentCounts.getModifiedBaseCounts(); + if(pos == 119094768) { + System.out.println(); + } + if (modificationCounts != null) { final byte base = (byte) 'C'; - final byte complement = (byte) 'G'; + final byte compl = (byte) 'G'; Map likelihoodSums = new HashMap<>(); Map modCounts = new HashMap<>(); @@ -146,20 +147,27 @@ private static void draw5MC(RenderContext context, if (likelihoodSums.size() > 0) { - // Count of bases at this location that could potentially be modified - double modifiableBaseCount = alignmentCounts.getPosCount(pos, base) + alignmentCounts.getNegCount(pos, complement); + // Special mode for out-of-spec 5mC CpG convention. + // Calls are made for the CG dinucleotide and only recorded on 1 strand. We adjust the height + // of the bar to account for the missing G- calls. This is an approximation and assumes the + // distribution of calls is ~ equal on both strands. + + // Is this a "C" or "G" reference site? We want to determine this from the counts data directly, this should work for all but pahtological edge cases + final int cCounts = alignmentCounts.getCount(pos, base); + final int gCounts = alignmentCounts.getCount(pos, compl); + final boolean cSite = cCounts > gCounts; + final int referenceCounts = cSite ? cCounts : gCounts; - // Compute "snp factor", ratio of count of base calls that could be modfied (on either strand) to + // Compute "snp factor", ratio of count of base calls that could be modfied (on either strand) to // total count. This is normally close to 1, but can be less due non CG bases at this location (e.g. snps) - double cgCount = alignmentCounts.getCount(pos, base) + alignmentCounts.getCount(pos, complement); - double snpFactor = cgCount / alignmentCounts.getTotalCount(pos); + double snpFactor = ((double) referenceCounts) / alignmentCounts.getTotalCount(pos); double calledBarHeight = snpFactor * barHeight; - double t = modifiableBaseCount * 255; // If all bases are called this is the total sum of all likelihoods, including "no mod" likelihood + double t = referenceCounts * 255; // If all bases are called this is the total sum of all likelihoods, including "no mod" likelihood // Likelihood of no modification. It is assumed that the likelihood of no modification == (1 - sum(likelihood)) - int c = Collections.max(modCounts.values()); - double noModProb = c * 255; + //int c = Collections.max(modCounts.values()); + double noModProb = t; for (String m : modCounts.keySet()) { if (likelihoodSums.containsKey(m)) { noModProb -= likelihoodSums.get(m); From f82370d9aa624782cb0dbc89fb2eeab886d3ea6f Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 22 Jun 2023 09:12:40 -0700 Subject: [PATCH 064/116] More robust gwas parser --- .../java/org/broad/igv/gwas/GWASParser.java | 69 ++++++++++++++----- .../org/broad/igv/tools/sort/GWASSorter.java | 5 +- 2 files changed, 57 insertions(+), 17 deletions(-) diff --git a/src/main/java/org/broad/igv/gwas/GWASParser.java b/src/main/java/org/broad/igv/gwas/GWASParser.java index f5ce15a74c..2ec5e6abff 100644 --- a/src/main/java/org/broad/igv/gwas/GWASParser.java +++ b/src/main/java/org/broad/igv/gwas/GWASParser.java @@ -25,6 +25,7 @@ package org.broad.igv.gwas; +import org.broad.igv.Globals; import org.broad.igv.logging.*; import org.broad.igv.exceptions.ParserException; import org.broad.igv.feature.genome.Genome; @@ -52,6 +53,12 @@ public class GWASParser { Genome genome; private GWASColumns columns; + private Pattern delimiterPattern; + + private int warningCount; + + private static int MAX_WARNING = 20; + public static boolean isGWASFile(String typeString) { return typeString.endsWith("logistic") || typeString.endsWith("linear") || typeString.endsWith("assoc") || typeString.endsWith("qassoc") || typeString.endsWith("gwas"); @@ -61,6 +68,7 @@ public GWASParser(ResourceLocator locator, Genome genome) { this.locator = locator; this.genome = genome; this.columns = new GWASColumns(); + this.warningCount = 0; } @@ -81,7 +89,10 @@ public Map> parse() throws IOException { String headerLine = reader.readLine(); - if (!this.columns.parseHeader(headerLine)) + // Try to determine delimiter pattern -- default is tab, but some older files might use whitespace + this.delimiterPattern = headerLine.indexOf('\t') > 0 ? Globals.tabPattern : Globals.whitespacePattern; + + if (!this.columns.parseHeader(headerLine, this.delimiterPattern)) throw new ParserException("Error while parsing header line.", 0, nextLine); while ((nextLine = reader.readLine()) != null && (nextLine.trim().length() > 0)) { @@ -89,7 +100,7 @@ public Map> parse() throws IOException { rowCounter++; GWASFeature f = null; f = parseLine(nextLine, rowCounter); - if(f != null) { + if (f != null) { List featureList = features.get(f.chr); if (featureList == null) { featureList = new ArrayList<>(); @@ -127,9 +138,22 @@ public Map> parse() throws IOException { */ private GWASFeature parseLine(String nextLine, long lineNumber) { - String[] tokens = nextLine.split("\\t|( +)"); + String[] tokens = this.delimiterPattern.split(nextLine); if (tokens.length >= 3) { + + + final String posString = tokens[this.columns.locationCol].trim(); + if(posString.indexOf(";") > 0 || posString.length() == 0 || posString.indexOf('x') > 0) { + if(warningCount < MAX_WARNING) { + log.warn(locator.getFileName() + " line number: " + lineNumber + ". Expected numeric position at column " + this.columns.locationCol + " Found " + tokens[this.columns.locationCol]); + } else if (warningCount == MAX_WARNING) { + log.warn("Max warning count excedeed for " + locator.getPath()); + } + warningCount++; + return null; + } + String chr = tokens[this.columns.chrCol].trim(); if (genome != null) { chr = genome.getCanonicalChrName(chr); @@ -137,21 +161,34 @@ private GWASFeature parseLine(String nextLine, long lineNumber) { int position; try { - position = Integer.parseInt(tokens[this.columns.locationCol].trim()); + + position = Integer.parseInt(posString) - 1; } catch (NumberFormatException e) { throw new ParserException("Column " + this.columns.locationCol + " must be a numeric value.", lineNumber, nextLine); } // Check if the p-value is NA if (!tokens[this.columns.pCol].trim().equalsIgnoreCase("NA")) { - double p; + double p = 0; try { - p = Double.parseDouble(tokens[this.columns.pCol]); - if (p <= 0) { - throw new NumberFormatException(); + // Check for extreme low values + String pvalString = tokens[this.columns.pCol]; + int idx = pvalString.indexOf("E"); + if(idx > 0) { + int exp = Integer.parseInt(pvalString.substring(idx+1)); + if (exp < log10(Double.MIN_VALUE)) { + p = -1 * exp; + } + } + + if(p == 0) { + p = Double.parseDouble(tokens[this.columns.pCol]); + if (p <= 0) { + throw new NumberFormatException(); + } + // Transform to -log10 + p = -log10(p); } - // Transform to -log10 - p = -log10(p); } catch (NumberFormatException e) { log.warn("Error parsing line number " + lineNumber + ". Column " + this.columns.pCol + " must be a positive numeric value. Found " + tokens[this.columns.pCol]); return null; @@ -182,12 +219,12 @@ public boolean hasAllFields() { * @param headerString * @return */ - public boolean parseHeader(String headerString) { + public boolean parseHeader(String headerString, Pattern delimiter) { + - Pattern whitespacePattern = Pattern.compile("\\s+"); try { headerString = headerString.trim(); - String[] headers = headerString.split("\\t|( +)"); + String[] headers = delimiter.split(headerString); this.columnHeaders = headers; int headersSize = headers.length; @@ -198,11 +235,11 @@ public boolean parseHeader(String headerString) { header = header.toLowerCase(); // Chromosome column - if (header.equals("chr") || header.equals("chromosome")) + if (header.equals("chr") || header.equals("chromosome") || header.equals("chr_id")) this.chrCol = colCounter; // Nucleotide position column - if (header.equals("bp") || header.equals("pos") || header.equals("position")) + if (header.equals("bp") || header.equals("pos") || header.equals("position") || header.equals("chr_pos")) this.locationCol = colCounter; // p-value column @@ -210,7 +247,7 @@ public boolean parseHeader(String headerString) { this.pCol = colCounter; // SNP identifier column - if (header.equals("snp") || header.equals("rs") || header.equals("rsid") || header.equals("rsnum") || header.equals("id") || header.equals("marker") || header.equals("markername")) + if (header.equals("snp") || header.equals("snps") || header.equals("rs") || header.equals("rsid") || header.equals("rsnum") || header.equals("id") || header.equals("marker") || header.equals("markername")) this.SNPCol = colCounter; } } catch (Exception e) { diff --git a/src/main/java/org/broad/igv/tools/sort/GWASSorter.java b/src/main/java/org/broad/igv/tools/sort/GWASSorter.java index bbf663c16a..9c2f225b31 100644 --- a/src/main/java/org/broad/igv/tools/sort/GWASSorter.java +++ b/src/main/java/org/broad/igv/tools/sort/GWASSorter.java @@ -25,6 +25,7 @@ package org.broad.igv.tools.sort; +import org.broad.igv.Globals; import org.broad.igv.logging.*; import org.broad.igv.gwas.GWASParser; import htsjdk.tribble.readers.AsciiLineReader; @@ -32,6 +33,7 @@ import java.io.*; import java.util.ArrayList; import java.util.List; +import java.util.regex.Pattern; /** * @author Jim Robinson @@ -59,7 +61,8 @@ private void findColumns(File inputFile) { while ((nextLine = br.readLine()) != null) { headerLines.add(nextLine); if (!nextLine.startsWith("#")) { - this.columns.parseHeader(nextLine); + Pattern delimiter = nextLine.indexOf('\t') > 0 ? Globals.tabPattern : Globals.whitespacePattern; + this.columns.parseHeader(nextLine, delimiter); break; } } From 6938f439d780ee73af1206c90db5a56d3353065f Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 22 Jun 2023 09:52:36 -0700 Subject: [PATCH 065/116] Gwas -- more warning less errors in parser. Set initial data range to 0-25 (-log10(pvalue)) --- src/main/java/org/broad/igv/gwas/GWASParser.java | 11 ++++++++--- src/main/java/org/broad/igv/gwas/GWASTrack.java | 12 +++++++----- 2 files changed, 15 insertions(+), 8 deletions(-) diff --git a/src/main/java/org/broad/igv/gwas/GWASParser.java b/src/main/java/org/broad/igv/gwas/GWASParser.java index 2ec5e6abff..32dbab7e76 100644 --- a/src/main/java/org/broad/igv/gwas/GWASParser.java +++ b/src/main/java/org/broad/igv/gwas/GWASParser.java @@ -146,7 +146,7 @@ private GWASFeature parseLine(String nextLine, long lineNumber) { final String posString = tokens[this.columns.locationCol].trim(); if(posString.indexOf(";") > 0 || posString.length() == 0 || posString.indexOf('x') > 0) { if(warningCount < MAX_WARNING) { - log.warn(locator.getFileName() + " line number: " + lineNumber + ". Expected numeric position at column " + this.columns.locationCol + " Found " + tokens[this.columns.locationCol]); + log.warn(locator.getFileName() + " line number: " + lineNumber + ". expected numeric position at column " + this.columns.locationCol + " Found " + tokens[this.columns.locationCol]); } else if (warningCount == MAX_WARNING) { log.warn("Max warning count excedeed for " + locator.getPath()); } @@ -161,10 +161,15 @@ private GWASFeature parseLine(String nextLine, long lineNumber) { int position; try { - position = Integer.parseInt(posString) - 1; } catch (NumberFormatException e) { - throw new ParserException("Column " + this.columns.locationCol + " must be a numeric value.", lineNumber, nextLine); + if(warningCount < MAX_WARNING) { + log.warn(locator.getFileName() + " line number: " + lineNumber + ". expected numeric position at column " + this.columns.locationCol + " Found " + tokens[this.columns.locationCol]); + } else if (warningCount == MAX_WARNING) { + log.warn("Max warning count excedeed for " + locator.getPath()); + } + warningCount++; + return null; } // Check if the p-value is NA diff --git a/src/main/java/org/broad/igv/gwas/GWASTrack.java b/src/main/java/org/broad/igv/gwas/GWASTrack.java index ca47f9ef9e..30b0d1a39c 100644 --- a/src/main/java/org/broad/igv/gwas/GWASTrack.java +++ b/src/main/java/org/broad/igv/gwas/GWASTrack.java @@ -104,10 +104,10 @@ public GWASTrack(ResourceLocator locator, IGVPreferences prefs = PreferencesManager.getPreferences(); - // Set range from 0 to highest value rounded to greater integer - setMaxValue(gData); - int mv = (int) Math.ceil(maxValue); - super.setDataRange(new DataRange(0, (mv / 2), mv)); + // Set inital range from 0 to highest value rounded to greater integer, or 25. This assumes pvalues + double maxValue = getMaxValue(gData); + int mv = Math.min(25, (int) Math.ceil(maxValue)); + super.setDataRange(new DataRange(0, mv)); // Get default values super.setHeight(prefs.getAsInt(Constants.GWAS_TRACK_HEIGHT)); @@ -126,12 +126,14 @@ public GWASTrack(ResourceLocator locator, public GWASTrack() { } - private void setMaxValue(Map> gData) { + private double getMaxValue(Map> gData) { + double maxValue = -1; for (List features : gData.values()) { for (GWASFeature f : features) { if (f.value > maxValue) maxValue = f.value; } } + return maxValue; } @Override From af1e36f8ff4c9f9cd094ead73548b4778909f64a Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 22 Jun 2023 10:04:47 -0700 Subject: [PATCH 066/116] GWAS -- parse attributes for popup text correctly for tab delimited files --- src/main/java/org/broad/igv/gwas/GWASParser.java | 11 +++++------ src/main/java/org/broad/igv/gwas/GWASTrack.java | 11 ++++++++--- src/main/java/org/broad/igv/track/TrackLoader.java | 3 +-- 3 files changed, 14 insertions(+), 11 deletions(-) diff --git a/src/main/java/org/broad/igv/gwas/GWASParser.java b/src/main/java/org/broad/igv/gwas/GWASParser.java index 32dbab7e76..6884bee985 100644 --- a/src/main/java/org/broad/igv/gwas/GWASParser.java +++ b/src/main/java/org/broad/igv/gwas/GWASParser.java @@ -53,7 +53,7 @@ public class GWASParser { Genome genome; private GWASColumns columns; - private Pattern delimiterPattern; + public Pattern delimiter; private int warningCount; @@ -90,16 +90,15 @@ public Map> parse() throws IOException { String headerLine = reader.readLine(); // Try to determine delimiter pattern -- default is tab, but some older files might use whitespace - this.delimiterPattern = headerLine.indexOf('\t') > 0 ? Globals.tabPattern : Globals.whitespacePattern; + this.delimiter = headerLine.indexOf('\t') > 0 ? Globals.tabPattern : Globals.whitespacePattern; - if (!this.columns.parseHeader(headerLine, this.delimiterPattern)) + if (!this.columns.parseHeader(headerLine, this.delimiter)) throw new ParserException("Error while parsing header line.", 0, nextLine); while ((nextLine = reader.readLine()) != null && (nextLine.trim().length() > 0)) { nextLine = nextLine.trim(); rowCounter++; - GWASFeature f = null; - f = parseLine(nextLine, rowCounter); + GWASFeature f = parseLine(nextLine, rowCounter); if (f != null) { List featureList = features.get(f.chr); if (featureList == null) { @@ -138,7 +137,7 @@ public Map> parse() throws IOException { */ private GWASFeature parseLine(String nextLine, long lineNumber) { - String[] tokens = this.delimiterPattern.split(nextLine); + String[] tokens = this.delimiter.split(nextLine); if (tokens.length >= 3) { diff --git a/src/main/java/org/broad/igv/gwas/GWASTrack.java b/src/main/java/org/broad/igv/gwas/GWASTrack.java index 30b0d1a39c..3d97744c76 100644 --- a/src/main/java/org/broad/igv/gwas/GWASTrack.java +++ b/src/main/java/org/broad/igv/gwas/GWASTrack.java @@ -53,6 +53,7 @@ import java.text.DecimalFormat; import java.util.List; import java.util.*; +import java.util.regex.Pattern; import java.util.stream.Collectors; /** @@ -81,6 +82,8 @@ public class GWASTrack extends AbstractTrack { private Map> gData; Genome genome; private String[] columns; + + private Pattern delimiter; IGV igv; /** @@ -96,9 +99,11 @@ public GWASTrack(ResourceLocator locator, String name, Map> gData, String[] columns, + Pattern delimiter, Genome genome) { super(locator, id, name); + this.delimiter = delimiter; this.genome = genome; this.igv = IGV.getInstance(); // TODO replace with parameter @@ -123,8 +128,8 @@ public GWASTrack(ResourceLocator locator, this.columns = columns; } - public GWASTrack() { - } + + private double getMaxValue(Map> gData) { double maxValue = -1; @@ -428,7 +433,7 @@ public String getDescriptionString(GWASFeature feature) { if (description != null) { descriptionString = ""; int headersSize = this.columns.length; - String[] tokens = Globals.singleTabMultiSpacePattern.split(description); + String[] tokens = this.delimiter.split(description); for (int i = 0; i < headersSize; i++) { String tmpHeaderToken = this.columns[i]; diff --git a/src/main/java/org/broad/igv/track/TrackLoader.java b/src/main/java/org/broad/igv/track/TrackLoader.java index 856eff84bc..3536acb808 100644 --- a/src/main/java/org/broad/igv/track/TrackLoader.java +++ b/src/main/java/org/broad/igv/track/TrackLoader.java @@ -545,8 +545,7 @@ private void loadGWASFile(ResourceLocator locator, List newTracks, Genome GWASParser gwasParser = new GWASParser(locator, genome); Map> gwasData = gwasParser.parse(); - - GWASTrack gwasTrack = new GWASTrack(locator, locator.getPath(), locator.getFileName(), gwasData, gwasParser.getColumnHeaders(), genome); + GWASTrack gwasTrack = new GWASTrack(locator, locator.getPath(), locator.getFileName(), gwasData, gwasParser.getColumnHeaders(), gwasParser.delimiter, genome); newTracks.add(gwasTrack); } From 8fffab09a4dd417e99ee5565bf85fd8be15a4dc3 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 22 Jun 2023 10:12:12 -0700 Subject: [PATCH 067/116] gwas - fix position parsing --- src/main/java/org/broad/igv/gwas/GWASParser.java | 2 +- src/main/java/org/broad/igv/gwas/GWASTrack.java | 4 ++-- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/src/main/java/org/broad/igv/gwas/GWASParser.java b/src/main/java/org/broad/igv/gwas/GWASParser.java index 6884bee985..52597641bc 100644 --- a/src/main/java/org/broad/igv/gwas/GWASParser.java +++ b/src/main/java/org/broad/igv/gwas/GWASParser.java @@ -160,7 +160,7 @@ private GWASFeature parseLine(String nextLine, long lineNumber) { int position; try { - position = Integer.parseInt(posString) - 1; + position = Integer.parseInt(posString); } catch (NumberFormatException e) { if(warningCount < MAX_WARNING) { log.warn(locator.getFileName() + " line number: " + lineNumber + ". expected numeric position at column " + this.columns.locationCol + " Found " + tokens[this.columns.locationCol]); diff --git a/src/main/java/org/broad/igv/gwas/GWASTrack.java b/src/main/java/org/broad/igv/gwas/GWASTrack.java index 3d97744c76..5a802557c3 100644 --- a/src/main/java/org/broad/igv/gwas/GWASTrack.java +++ b/src/main/java/org/broad/igv/gwas/GWASTrack.java @@ -228,9 +228,9 @@ else if (this.alternatingColors) { // Get location, e.g. start for the data point int start; if (chrName.equalsIgnoreCase("all")) - start = genome.getGenomeCoordinate(feature.chr, feature.position); + start = genome.getGenomeCoordinate(feature.chr, feature.position - 1); else - start = feature.position; + start = feature.position - 1; // Based on location, calculate X-coordinate, or break if outside of the view double pX = ((start - origin) / locScale); From 8d47abf45f7f2fe5662b8d5a01b7a09474d892bf Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Fri, 23 Jun 2023 22:23:45 -0700 Subject: [PATCH 068/116] misc gwas improvements (#1347) --- .../java/org/broad/igv/gwas/GWASData.java | 61 ++++ .../java/org/broad/igv/gwas/GWASFeature.java | 1 + .../java/org/broad/igv/gwas/GWASParser.java | 44 +-- .../java/org/broad/igv/gwas/GWASTrack.java | 317 +++++++----------- .../java/org/broad/igv/track/TrackLoader.java | 3 +- .../org/broad/igv/gwas/GWASParserTest.java | 6 +- 6 files changed, 191 insertions(+), 241 deletions(-) create mode 100644 src/main/java/org/broad/igv/gwas/GWASData.java diff --git a/src/main/java/org/broad/igv/gwas/GWASData.java b/src/main/java/org/broad/igv/gwas/GWASData.java new file mode 100644 index 0000000000..6abd7fb4a4 --- /dev/null +++ b/src/main/java/org/broad/igv/gwas/GWASData.java @@ -0,0 +1,61 @@ +package org.broad.igv.gwas; + +import java.util.*; + +/** + * Simple map-like class that contains GWAS pvalue data organized by chromosome, along with min and max values* + */ + +public class GWASData { + + double minValue; + double maxValue; + Map> features; + + public GWASData() { + this.features = new HashMap<>(); + minValue = Double.MAX_VALUE; + maxValue = - Double.MAX_VALUE; + } + + void addFeature(GWASFeature f) { + List featureList = features.get(f.chr); + if (featureList == null) { + featureList = new ArrayList<>(); + features.put(f.chr, featureList); + } + featureList.add(f); + minValue = Math.min(minValue, f.value); + maxValue = Math.max(maxValue, f.value); + } + + Set keySet() { + return features.keySet(); + } + Collection> values() { + return features.values(); + } + + List get(String chr) { + return features.get(chr); + } + + boolean isEmpty() { + return features.isEmpty(); + } + + public double getMinValue() { + return minValue; + } + + public double getMaxValue() { + return maxValue; + } + + void finish() { + // Sort features by position + for (List featureList : features.values()) { + featureList.sort(Comparator.comparingInt(o -> o.position)); + } + } +} diff --git a/src/main/java/org/broad/igv/gwas/GWASFeature.java b/src/main/java/org/broad/igv/gwas/GWASFeature.java index a0ddc15732..b44719dcd8 100644 --- a/src/main/java/org/broad/igv/gwas/GWASFeature.java +++ b/src/main/java/org/broad/igv/gwas/GWASFeature.java @@ -11,6 +11,7 @@ public class GWASFeature { String line; int pixelX; int pixelY; + int pixelSize; public GWASFeature(String chr, int position, double value, String line) { this.chr = chr; diff --git a/src/main/java/org/broad/igv/gwas/GWASParser.java b/src/main/java/org/broad/igv/gwas/GWASParser.java index 52597641bc..d63ed2c6d1 100644 --- a/src/main/java/org/broad/igv/gwas/GWASParser.java +++ b/src/main/java/org/broad/igv/gwas/GWASParser.java @@ -77,12 +77,12 @@ public String[] getColumnHeaders() { } - public Map> parse() throws IOException { + public GWASData parse() throws IOException { BufferedReader reader = null; String nextLine = null; int rowCounter = 0; - Map> features = new HashMap<>(); + GWASData data = new GWASData(); try { reader = ParsingUtils.openBufferedReader(locator); @@ -98,23 +98,13 @@ public Map> parse() throws IOException { while ((nextLine = reader.readLine()) != null && (nextLine.trim().length() > 0)) { nextLine = nextLine.trim(); rowCounter++; - GWASFeature f = parseLine(nextLine, rowCounter); + GWASFeature f = parseLine(nextLine, rowCounter); if (f != null) { - List featureList = features.get(f.chr); - if (featureList == null) { - featureList = new ArrayList<>(); - features.put(f.chr, featureList); - } - featureList.add(f); + data.addFeature(f); } } - - // Sort features by position - for (List featureList : features.values()) { - featureList.sort(Comparator.comparingInt(o -> o.position)); - } - - return features; + data.finish(); + return data; } catch (Exception e) { if (nextLine != null && rowCounter != 0) { @@ -143,8 +133,8 @@ private GWASFeature parseLine(String nextLine, long lineNumber) { final String posString = tokens[this.columns.locationCol].trim(); - if(posString.indexOf(";") > 0 || posString.length() == 0 || posString.indexOf('x') > 0) { - if(warningCount < MAX_WARNING) { + if (posString.indexOf(";") > 0 || posString.length() == 0 || posString.indexOf('x') > 0) { + if (warningCount < MAX_WARNING) { log.warn(locator.getFileName() + " line number: " + lineNumber + ". expected numeric position at column " + this.columns.locationCol + " Found " + tokens[this.columns.locationCol]); } else if (warningCount == MAX_WARNING) { log.warn("Max warning count excedeed for " + locator.getPath()); @@ -162,7 +152,7 @@ private GWASFeature parseLine(String nextLine, long lineNumber) { try { position = Integer.parseInt(posString); } catch (NumberFormatException e) { - if(warningCount < MAX_WARNING) { + if (warningCount < MAX_WARNING) { log.warn(locator.getFileName() + " line number: " + lineNumber + ". expected numeric position at column " + this.columns.locationCol + " Found " + tokens[this.columns.locationCol]); } else if (warningCount == MAX_WARNING) { log.warn("Max warning count excedeed for " + locator.getPath()); @@ -178,14 +168,14 @@ private GWASFeature parseLine(String nextLine, long lineNumber) { // Check for extreme low values String pvalString = tokens[this.columns.pCol]; int idx = pvalString.indexOf("E"); - if(idx > 0) { - int exp = Integer.parseInt(pvalString.substring(idx+1)); + if (idx > 0) { + int exp = Integer.parseInt(pvalString.substring(idx + 1)); if (exp < log10(Double.MIN_VALUE)) { p = -1 * exp; } } - if(p == 0) { + if (p == 0) { p = Double.parseDouble(tokens[this.columns.pCol]); if (p <= 0) { throw new NumberFormatException(); @@ -262,14 +252,4 @@ public boolean parseHeader(String headerString, Pattern delimiter) { } } - public static void main(String[] args) throws IOException { - GWASParser parser = new GWASParser(new ResourceLocator("test/data/gwas/smallp.gwas"), null); - Map> data = parser.parse(); - for (List features : data.values()) { - for (GWASFeature f : features) { - double val = f.value; - System.out.println(val); - } - } - } } \ No newline at end of file diff --git a/src/main/java/org/broad/igv/gwas/GWASTrack.java b/src/main/java/org/broad/igv/gwas/GWASTrack.java index 5a802557c3..04fa354616 100644 --- a/src/main/java/org/broad/igv/gwas/GWASTrack.java +++ b/src/main/java/org/broad/igv/gwas/GWASTrack.java @@ -73,13 +73,11 @@ public class GWASTrack extends AbstractTrack { private boolean alternatingColors; private Color primaryColor; private Color secondaryColor; - private double trackMinY; private double maxY; - private double scale; private boolean drawYAxis = true; private boolean showAxis = true; double maxValue = -1; - private Map> gData; + private GWASData gData; Genome genome; private String[] columns; @@ -97,7 +95,7 @@ public class GWASTrack extends AbstractTrack { public GWASTrack(ResourceLocator locator, String id, String name, - Map> gData, + GWASData gData, String[] columns, Pattern delimiter, Genome genome) { @@ -110,9 +108,9 @@ public GWASTrack(ResourceLocator locator, IGVPreferences prefs = PreferencesManager.getPreferences(); // Set inital range from 0 to highest value rounded to greater integer, or 25. This assumes pvalues - double maxValue = getMaxValue(gData); - int mv = Math.min(25, (int) Math.ceil(maxValue)); - super.setDataRange(new DataRange(0, mv)); + float maxValue = Math.min(25, (float) Math.ceil(gData.getMaxValue())); + float minValue = (float) Math.floor(gData.getMinValue()); + super.setDataRange(new DataRange(minValue, maxValue)); // Get default values super.setHeight(prefs.getAsInt(Constants.GWAS_TRACK_HEIGHT)); @@ -128,19 +126,6 @@ public GWASTrack(ResourceLocator locator, this.columns = columns; } - - - - private double getMaxValue(Map> gData) { - double maxValue = -1; - for (List features : gData.values()) { - for (GWASFeature f : features) { - if (f.value > maxValue) maxValue = f.value; - } - } - return maxValue; - } - @Override public boolean isNumeric() { return true; @@ -156,207 +141,140 @@ public void load(ReferenceFrame frame) { // Nothing to do, track is initialized with all data } - public void render(RenderContext context, Rectangle arect) { + public void render(RenderContext context, Rectangle rect) { Genome genome = GenomeManager.getInstance().getCurrentGenome(); - this.trackMinY = arect.getMinY(); - Rectangle adjustedRect = calculateDrawingRect(arect); - double adjustedRectMaxX = adjustedRect.getMaxX(); - double adjustedRectMaxY = adjustedRect.getMaxY(); - double adjustedRectY = adjustedRect.getY(); - this.maxY = adjustedRectMaxY; - this.scale = context.getScale(); - int bufferX = (int) adjustedRectMaxX; - int bufferY = (int) adjustedRectMaxY; - Color[][] drawBuffer = new Color[bufferX + 1][bufferY + 1]; + double origin = context.getOrigin(); double locScale = context.getScale(); - // Get the Y axis definition, consisting of minimum, maximum, and base value. Often - // the base value is == min value which is == 0. + Graphics2D g = null; - DataRange axisDefinition = this.getDataRange(); - float maxValue = axisDefinition.getMaximum(); - float minValue = axisDefinition.getMinimum(); + try { + g = (Graphics2D) context.getGraphics().create(); + g.setClip(rect); - // Calculate the Y scale factor. - double yScaleFactor = adjustedRect.getHeight() / (maxValue - minValue); + Rectangle plotRect = calculateDrawingRect(rect); - //int lastPx = 0; - String chrName = context.getChr(); - List chrList; - if (chrName.equals("All")) { - chrList = genome.getLongChromosomeNames(); - } else { - chrList = Arrays.asList(chrName); - } - double dx = Math.ceil(1 / locScale) + 1; - double rangeMaxValue = Math.ceil(maxValue); + DataRange dataRange = this.getDataRange(); + float maxValue = dataRange.getMaximum(); + float minValue = dataRange.getMinimum(); + double pointSizeScaleFactor = ((double) (this.maxPointSize - this.minPointSize)) / (maxValue - minValue); + + String chrName = context.getChr(); + List chrList; + if (chrName.equals("All")) { + chrList = genome.getLongChromosomeNames(); + } else { + chrList = Arrays.asList(chrName); + } - double pointSizeScale = rangeMaxValue / maxPointSize; + Color drawColor = this.primaryColor; - Color drawColor = this.primaryColor; - int xMinPointSize = (int) (1 / locScale); + // Loop through data points, chromosome by chromosome - // Loop through data points, chromosome by chromosome + int chrCounter = 0; + for (String chr : chrList) { - int chrCounter = 0; - for (String chr : chrList) { + List featureList = gData.get(chr); - List featureList = gData.get(chr); + if (featureList != null) { - if (featureList != null) { + if (this.useChrColors) + drawColor = ChromosomeColors.getColor(chr); + else if (this.alternatingColors) { + if (chrCounter % 2 == 0) + drawColor = this.secondaryColor; + else + drawColor = this.primaryColor; + } - // Choose a color for the chromosome - // Use specific color for each chromosome - if (this.useChrColors) - drawColor = ChromosomeColors.getColor(chr); + // Loop through data points + for (GWASFeature feature : featureList) { - // Use two alternating colors for chromosomes - else if (this.alternatingColors) { - if (chrCounter % 2 == 0) - drawColor = this.secondaryColor; - else - drawColor = this.primaryColor; - } + // Get location, e.g. start for the data point + int start; + if (chrName.equalsIgnoreCase("all")) + start = genome.getGenomeCoordinate(feature.chr, feature.position) - 1; + else + start = feature.position - 1; + + + // Based on value of the data point, calculate Y-coordinate + double dataY = feature.value; - // Loop through data points in a chromosome - Graphics2D g = context.getGraphics(); - for (GWASFeature feature : featureList) { - - // Get location, e.g. start for the data point - int start; - if (chrName.equalsIgnoreCase("all")) - start = genome.getGenomeCoordinate(feature.chr, feature.position - 1); - else - start = feature.position - 1; - - // Based on location, calculate X-coordinate, or break if outside of the view - double pX = ((start - origin) / locScale); - if ((pX + dx < 0)) - continue; - else if (pX > adjustedRectMaxX) - break; - - // Based on value of the data point, calculate Y-coordinate - double dataY = feature.value; - - if (!Double.isNaN(dataY)) { - - int xPointSize = (int) Math.ceil(dataY / pointSizeScale); - - // Scale y size based on the used range, data value and max point size - int yPointSize = xPointSize; - if (yPointSize < minPointSize) - yPointSize = minPointSize; - - // If x minimum size is smaller than point minimum size, use minimum point size - if (xMinPointSize < minPointSize) - xMinPointSize = minPointSize; - - if (xPointSize < xMinPointSize) - xPointSize = xMinPointSize; - - // Point sizes divided by two to center locations of large points - int x = (int) pX - (xPointSize / 2); - int y = ((int) Math.min(adjustedRectMaxY, adjustedRectY + (maxValue - dataY) * yScaleFactor)) - (yPointSize / 2); - - int maxDrawX = x + xPointSize; - int maxDrawY = y + yPointSize; - if (x < 0) - x = 0; - if (y < 0) - y = 0; - - if (maxDrawX > adjustedRectMaxX) - maxDrawX = (int) adjustedRectMaxX; - if (maxDrawY > adjustedRectMaxY) - maxDrawY = (int) adjustedRectMaxY; - - g.setColor(drawColor); - g.fillRect(x, y, maxDrawX - x, maxDrawY - y); - feature.pixelX = (x + maxDrawX) / 2; - feature.pixelY = (y + maxDrawY) / 2; + if (!Double.isNaN(dataY)) { + + int pixelSize = Math.min(this.maxPointSize, this.minPointSize + (int) (pointSizeScaleFactor * (dataY - minValue))); + int x = (int) ((start - origin + 0.5) / locScale) - pixelSize / 2; + int y = computeYPixelValue(plotRect, this.dataRange, dataY) - pixelSize / 2; + g.setColor(drawColor); + g.fillOval(x, y, pixelSize, pixelSize); + feature.pixelX = x; + feature.pixelY = y; + feature.pixelSize = pixelSize; + } } } + chrCounter++; + } + + // Draw the legend axis + if (showAxis) { + this.renderAxis(context, plotRect); } - chrCounter++; - } - // Draw the legend axis - if (showAxis) { - this.renderAxis(context, arect); + } finally { + if (g != null) g.dispose(); } } - void renderAxis(RenderContext context, Rectangle arect) { + void renderAxis(RenderContext context, Rectangle plotRect) { - Rectangle drawingRect = calculateDrawingRect(arect); - - Color labelColor = PreferencesManager.getPreferences().getAsBoolean(Constants.CHART_COLOR_TRACK_NAME) ? getColor() : Color.black; - Graphics2D labelGraphics = context.getGraphic2DForColor(labelColor); - //Graphics2D labelGraphics = context.getGraphic2DForColor(Color.black); - - labelGraphics.setFont(FontManager.getFont(8)); - - String tmpDisplayName = this.getDisplayName(); - if (tmpDisplayName != null && tmpDisplayName.length() > 0 && PreferencesManager.getPreferences().getAsBoolean(Constants.CHART_DRAW_TRACK_NAME)) { - // Only attempt if track height is > 25 pixels - if (arect.getHeight() > 25) { - Rectangle labelRect = new Rectangle(arect.x, arect.y + 10, arect.width, 10); - labelGraphics.setFont(FontManager.getFont(10)); - GraphicUtils.drawCenteredText(tmpDisplayName, labelRect, labelGraphics); - } - } + Graphics2D g = null; - //if (IGVPreferences.getInstance().getAsBoolean(IGVPreferences.CHART_DRAW_Y_AXIS)) { - if (this.drawYAxis) { - labelGraphics = context.getGraphic2DForColor(Color.black); - labelGraphics.setFont(FontManager.getFont(11)); + try { + g = (Graphics2D) context.getGraphics().create(); + g.setColor(Color.black); + g.setFont(FontManager.getFont(11)); - Rectangle axisRect = new Rectangle(arect.x, arect.y + 1, AXIS_AREA_WIDTH, arect.height); + Rectangle axisRect = new Rectangle(plotRect.x, plotRect.y + 1, AXIS_AREA_WIDTH, plotRect.height); DataRange axisDefinition = getDataRange(); float maxValue = axisDefinition.getMaximum(); float minValue = axisDefinition.getMinimum(); - // Bottom (minimum tick mark) - int pY = computeYPixelValue(drawingRect, axisDefinition, minValue); + int pY = computeYPixelValue(plotRect, axisDefinition, minValue); - labelGraphics.drawLine(axisRect.x + AXIS_AREA_WIDTH - 10, pY, - axisRect.x + AXIS_AREA_WIDTH - 5, pY); - GraphicUtils.drawRightJustifiedText(formatter.format(minValue), - axisRect.x + AXIS_AREA_WIDTH - 15, pY, labelGraphics); + g.drawLine(axisRect.x + AXIS_AREA_WIDTH - 10, pY, axisRect.x + AXIS_AREA_WIDTH - 5, pY); + GraphicUtils.drawRightJustifiedText(formatter.format(minValue), axisRect.x + AXIS_AREA_WIDTH - 15, pY, g); // Top (maximum tick mark) - int topPY = computeYPixelValue(drawingRect, axisDefinition, maxValue); + int topPY = computeYPixelValue(plotRect, axisDefinition, maxValue); - labelGraphics.drawLine(axisRect.x + AXIS_AREA_WIDTH - 10, topPY, - axisRect.x + AXIS_AREA_WIDTH - 5, topPY); - GraphicUtils.drawRightJustifiedText(formatter.format(maxValue), - axisRect.x + AXIS_AREA_WIDTH - 15, topPY + 4, labelGraphics); + g.drawLine(axisRect.x + AXIS_AREA_WIDTH - 10, topPY, axisRect.x + AXIS_AREA_WIDTH - 5, topPY); + GraphicUtils.drawRightJustifiedText(formatter.format(maxValue), axisRect.x + AXIS_AREA_WIDTH - 15, topPY + 4, g); // Connect top and bottom - labelGraphics.drawLine(axisRect.x + AXIS_AREA_WIDTH - 10, topPY, - axisRect.x + AXIS_AREA_WIDTH - 10, pY); + g.drawLine(axisRect.x + AXIS_AREA_WIDTH - 10, topPY, axisRect.x + AXIS_AREA_WIDTH - 10, pY); // Draw middle tick marks from top to bottom, draw only if space available int lastY = pY; for (int i = (int) minValue + 1; i < (int) maxValue; i++) { - int midPY = computeYPixelValue(drawingRect, axisDefinition, i); + int midPY = computeYPixelValue(plotRect, axisDefinition, i); if ((midPY < lastY - 15) && (midPY < pY - 15) && (midPY > topPY + 15)) { - labelGraphics.drawLine(axisRect.x + AXIS_AREA_WIDTH - 10, midPY, - axisRect.x + AXIS_AREA_WIDTH - 5, midPY); - GraphicUtils.drawRightJustifiedText(formatter.format(i), - axisRect.x + AXIS_AREA_WIDTH - 15, midPY + 4, labelGraphics); + g.drawLine(axisRect.x + AXIS_AREA_WIDTH - 10, midPY, axisRect.x + AXIS_AREA_WIDTH - 5, midPY); + GraphicUtils.drawRightJustifiedText(formatter.format(i), axisRect.x + AXIS_AREA_WIDTH - 15, midPY + 4, g); lastY = midPY; } } + } finally { + if (g != null) g.dispose(); } } @@ -386,41 +304,36 @@ Rectangle calculateDrawingRect(Rectangle arect) { * Find index of data point closest to given chromosomal location and y-coordinate */ GWASFeature findFeature(String chr, int mouseX, int mouseY) { - final int pixelThreshold = 3; - List featureList; - if ("all".equalsIgnoreCase(chr)) { - featureList = new ArrayList<>(); - for (List chrFeatures : this.gData.values()) { - for (GWASFeature f : chrFeatures) { - if (Math.abs(mouseX - f.pixelX) <= pixelThreshold && Math.abs(mouseY - f.pixelY) <= pixelThreshold) { - featureList.add(f); - } + + List closeFeatures; + Collection chromosomes = "all".equalsIgnoreCase(chr) ? this.gData.keySet() : Arrays.asList(chr); + closeFeatures = new ArrayList<>(); + for (String c : chromosomes) { + List chrFeatures = this.gData.get(c); + for (GWASFeature f : chrFeatures) { + int pixelSize = Math.max(3, f.pixelSize); + if (Math.abs(mouseX - f.pixelX) <= pixelSize && Math.abs(mouseY - f.pixelY) <= pixelSize) { + closeFeatures.add(f); } } - } else { - featureList = this.gData.get(chr); } - if (featureList == null) { + if (closeFeatures.isEmpty()) { return null; + } else if (closeFeatures.size() == 1) { + return closeFeatures.get(0); } else { - List closeFeatures = featureList.stream() - .filter(f -> Math.abs(mouseX - f.pixelX) <= pixelThreshold && Math.abs(mouseY - f.pixelY) <= pixelThreshold) - .collect(Collectors.toList()); - if (closeFeatures.isEmpty()) { - return null; - } else { - closeFeatures.sort((o1, o2) -> { - double d1 = Math.sqrt((mouseX - o1.pixelX) * (mouseX - o1.pixelX) + (mouseY - o1.pixelY) * (mouseY - o1.pixelY)); - double d2 = Math.sqrt((mouseX - o2.pixelX) * (mouseX - o2.pixelX) + (mouseY - o2.pixelY) * (mouseY - o2.pixelY)); - if (d1 == d2) return 0; - else if (d1 > d2) return 1; - else return -1; - }); - return closeFeatures.get(0); - } + closeFeatures.sort((o1, o2) -> { + double d1 = Math.sqrt((mouseX - o1.pixelX) * (mouseX - o1.pixelX) + (mouseY - o1.pixelY) * (mouseY - o1.pixelY)); + double d2 = Math.sqrt((mouseX - o2.pixelX) * (mouseX - o2.pixelX) + (mouseY - o2.pixelY) * (mouseY - o2.pixelY)); + if (d1 == d2) return 0; + else if (d1 > d2) return 1; + else return -1; + }); + return closeFeatures.get(0); } } + /** * Get description for a data point at given chromosome and index. If description is not cached, re-populate cache. */ @@ -703,12 +616,9 @@ public void marshalXML(Document document, Element element) { element.setAttribute("maxPointSize", String.valueOf(maxPointSize)); element.setAttribute("minPointSize", String.valueOf(minPointSize)); - element.setAttribute("scale", String.valueOf(scale)); - element.setAttribute("trackMinY", String.valueOf(trackMinY)); - element.setAttribute("maxY", String.valueOf(maxY)); element.setAttribute("useChrColors", String.valueOf(useChrColors)); element.setAttribute("singleColor", String.valueOf(singleColor)); - element.setAttribute("drawYAxis", String.valueOf(drawYAxis)); + element.setAttribute("showAxis", String.valueOf(showAxis)); element.setAttribute("alternatingColors", String.valueOf(alternatingColors)); if (primaryColor != null) { element.setAttribute("primaryColor", ColorUtilities.colorToString(primaryColor)); @@ -727,12 +637,9 @@ public void unmarshalXML(Element element, Integer version) { maxPointSize = Integer.parseInt(element.getAttribute("maxPointSize")); minPointSize = Integer.parseInt(element.getAttribute("minPointSize")); - scale = Double.parseDouble(element.getAttribute("scale")); - trackMinY = Double.parseDouble(element.getAttribute("trackMinY")); - maxY = Double.parseDouble(element.getAttribute("maxY")); useChrColors = Boolean.parseBoolean(element.getAttribute("useChrColors")); singleColor = Boolean.parseBoolean(element.getAttribute("singleColor")); - drawYAxis = Boolean.parseBoolean(element.getAttribute("drawYAxis")); + showAxis = Boolean.parseBoolean(element.getAttribute("showAxis")) || Boolean.parseBoolean(element.getAttribute("drawYAxis")); alternatingColors = Boolean.parseBoolean(element.getAttribute("alternatingColors")); if (element.hasAttribute("primaryColor")) { primaryColor = ColorUtilities.stringToColor(element.getAttribute("primaryColor")); diff --git a/src/main/java/org/broad/igv/track/TrackLoader.java b/src/main/java/org/broad/igv/track/TrackLoader.java index 3536acb808..03254f5707 100644 --- a/src/main/java/org/broad/igv/track/TrackLoader.java +++ b/src/main/java/org/broad/igv/track/TrackLoader.java @@ -58,6 +58,7 @@ import org.broad.igv.feature.tribble.FeatureFileHeader; import org.broad.igv.feature.tribble.GFFCodec; import org.broad.igv.feature.tribble.TribbleIndexNotFoundException; +import org.broad.igv.gwas.GWASData; import org.broad.igv.util.GoogleUtils; import org.broad.igv.gwas.GWASFeature; import org.broad.igv.gwas.GWASParser; @@ -544,7 +545,7 @@ private void loadDSIFile(ResourceLocator locator, List newTracks, Genome private void loadGWASFile(ResourceLocator locator, List newTracks, Genome genome) throws IOException { GWASParser gwasParser = new GWASParser(locator, genome); - Map> gwasData = gwasParser.parse(); + GWASData gwasData = gwasParser.parse(); GWASTrack gwasTrack = new GWASTrack(locator, locator.getPath(), locator.getFileName(), gwasData, gwasParser.getColumnHeaders(), gwasParser.delimiter, genome); newTracks.add(gwasTrack); diff --git a/src/test/java/org/broad/igv/gwas/GWASParserTest.java b/src/test/java/org/broad/igv/gwas/GWASParserTest.java index 8540f6ee8f..6adc111416 100644 --- a/src/test/java/org/broad/igv/gwas/GWASParserTest.java +++ b/src/test/java/org/broad/igv/gwas/GWASParserTest.java @@ -44,7 +44,7 @@ public class GWASParserTest extends AbstractHeadlessTest { @Test public void testParse_underflows() throws Exception { GWASParser parser = new GWASParser(new ResourceLocator(TestUtils.DATA_DIR + "gwas/smallp.gwas"), genome); - Map> data = parser.parse(); + GWASData data = parser.parse(); for (List features : data.values()) { for (GWASFeature f : features) { double val = f.value; @@ -58,7 +58,7 @@ public void testParse_underflows() throws Exception { @Test public void testParseStarts() throws Exception { GWASParser parser = new GWASParser(new ResourceLocator(TestUtils.DATA_DIR + "gwas/smallp.gwas"), genome); - Map> data = parser.parse(); + GWASData data = parser.parse(); List features = data.get("chr6"); int[] expStarts = {29622220, 29623739, 29623739}; @@ -79,7 +79,7 @@ public void tstParseBad(String finame) throws Exception { GWASParser parser = new GWASParser(new ResourceLocator(TestUtils.DATA_DIR + "gwas/" + finame), genome); boolean excepted = false; - Map> data = parser.parse(); + GWASData data = parser.parse(); assertTrue(data.isEmpty()); From d885f74e60e57ce742de56c3b39d00b13e72ee1b Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Sat, 24 Jun 2023 21:45:42 -0700 Subject: [PATCH 069/116] Add some comments --- .../BaseModificationCoverageRenderer.java | 21 ++++++++----------- 1 file changed, 9 insertions(+), 12 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java index 21b7667388..5c0a73596e 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java @@ -76,15 +76,15 @@ private static void draw(RenderContext context, float modFraction; if (cpgMode & "m".equals(modification)) { // Special mode for out-of-spec 5mC CpG convention. - // Calls are made for the CG dinucleotide and only recorded on 1 strand. We adjust the height - // of the bar to account for the missing G- calls. This is an approximation and assumes the - // distribution of calls is ~ equal on both strands. - final byte base = (byte) 'C'; //key.getCanonicalBase(); - final byte compl = (byte) 'G'; //SequenceUtil.complement(base); + // Calls are made for the CG dinucleotide and only recorded for the canonical "C". We adjust the height + // of the bar to account for the missing G- calls. + final byte base = (byte) 'C'; + final byte compl = (byte) 'G'; final int posCount = alignmentCounts.getPosCount(pos, base); final int negCount = alignmentCounts.getNegCount(pos, compl); - // Is this a "C" or "G" reference site? We want to determine this from the counts data directly, this should work for all but pahtological edge cases + // Is this a "C" or "G" reference site? We want to determine this from the counts data + // directly, this should work for all but pathological edge cases final int cCounts = alignmentCounts.getCount(pos, base); final int gCounts = alignmentCounts.getCount(pos, compl); final boolean cSite = cCounts > gCounts; @@ -119,10 +119,6 @@ private static void draw5MC(RenderContext context, BaseModificationCounts modificationCounts = alignmentCounts.getModifiedBaseCounts(); - if(pos == 119094768) { - System.out.println(); - } - if (modificationCounts != null) { final byte base = (byte) 'C'; @@ -152,13 +148,14 @@ private static void draw5MC(RenderContext context, // of the bar to account for the missing G- calls. This is an approximation and assumes the // distribution of calls is ~ equal on both strands. - // Is this a "C" or "G" reference site? We want to determine this from the counts data directly, this should work for all but pahtological edge cases + // Is this a "C" or "G" reference site? We want to determine this from the counts data directly, + // this should work for all but pathological edge cases final int cCounts = alignmentCounts.getCount(pos, base); final int gCounts = alignmentCounts.getCount(pos, compl); final boolean cSite = cCounts > gCounts; final int referenceCounts = cSite ? cCounts : gCounts; - // Compute "snp factor", ratio of count of base calls that could be modfied (on either strand) to + // Compute "snp factor", ratio of count of base calls that could be modified to // total count. This is normally close to 1, but can be less due non CG bases at this location (e.g. snps) double snpFactor = ((double) referenceCounts) / alignmentCounts.getTotalCount(pos); From 337d8ccabb210c44e4a80f1ef05bb6fb2ec48754 Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Tue, 27 Jun 2023 12:35:24 -0700 Subject: [PATCH 070/116] basemod enhancements -- allow selection of specific modifications (#1349) --- .../broad/igv/sam/AlignmentDataManager.java | 25 +++++++- .../org/broad/igv/sam/AlignmentRenderer.java | 2 +- .../org/broad/igv/sam/AlignmentTrack.java | 34 ++++++++--- .../org/broad/igv/sam/AlignmentTrackMenu.java | 61 ++++++++++++++----- .../java/org/broad/igv/sam/CoverageTrack.java | 3 +- .../BaseModificationCoverageRenderer.java | 5 +- .../sam/mods/BaseModificationRenderer.java | 5 +- .../igv/sam/mods/BaseModificationUtils.java | 8 ++- 8 files changed, 110 insertions(+), 33 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentDataManager.java b/src/main/java/org/broad/igv/sam/AlignmentDataManager.java index d2c351eba2..6412852b53 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentDataManager.java +++ b/src/main/java/org/broad/igv/sam/AlignmentDataManager.java @@ -36,6 +36,7 @@ import org.broad.igv.feature.genome.Genome; import org.broad.igv.prefs.IGVPreferences; import org.broad.igv.prefs.PreferencesManager; +import org.broad.igv.sam.mods.BaseModificationSet; import org.broad.igv.sam.reader.AlignmentReader; import org.broad.igv.sam.reader.AlignmentReaderFactory; import org.broad.igv.track.Track; @@ -58,17 +59,18 @@ public class AlignmentDataManager implements IGVEventObserver { private static Logger log = LogManager.getLogger(AlignmentDataManager.class); private final AlignmentReader reader; - private AlignmentTrack alignmentTrack; private CoverageTrack coverageTrack; private Set subscribedTracks; - private List intervalCache; private ResourceLocator locator; private HashMap chrMappings = new HashMap(); private AlignmentTileLoader loader; private Map peStats; private SpliceJunctionHelper.LoadOptions loadOptions; + + private Set allBaseModifications = new HashSet<>(); + private Range currentlyLoading; public AlignmentDataManager(ResourceLocator locator, Genome genome) throws IOException { @@ -193,6 +195,13 @@ public Map getPEStats() { return peStats; } + /** + * Return all base modfications seen in loaded alignments + */ + public Set getAllBaseModifications() { + return allBaseModifications; + } + public boolean isPairedEnd() { return getLoader().isPairedEnd(); } @@ -393,9 +402,21 @@ AlignmentInterval loadInterval(String chr, int start, int end, AlignmentTrack.Re downsampleOptions, peStats, bisulfiteContext, renderOptions); List alignments = t.getAlignments(); List downsampledIntervals = t.getDownsampledIntervals(); + this.updateBaseModfications(alignments); return new AlignmentInterval(chr, start, end, alignments, t.getCounts(), spliceJunctionHelper, downsampledIntervals); } + private void updateBaseModfications(List alignments) { + for(Alignment a : alignments) { + List bmSets = a.getBaseModificationSets(); + if(bmSets != null) { + for(BaseModificationSet bms : bmSets) { + allBaseModifications.add(bms.getModification()); + } + } + } + } + public AlignmentTrack.ExperimentType inferType() { ReadStats readStats = new ReadStats(); List sample = AlignmentUtils.firstAlignments(reader, 100); diff --git a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java index 10ccaab96e..b09f76004d 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java +++ b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java @@ -853,7 +853,7 @@ private void drawAlignment( // Base modification if (colorOption.isBaseMod()) { - BaseModificationRenderer.drawModifications(alignment, bpStart, locScale, rowRect, context.getGraphics(), colorOption); + BaseModificationRenderer.drawModifications(alignment, bpStart, locScale, rowRect, context.getGraphics(), colorOption, renderOptions.getBasemodFilter()); } // Kinetic data diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrack.java b/src/main/java/org/broad/igv/sam/AlignmentTrack.java index 93b3d57c7c..0cb4c7222d 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrack.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrack.java @@ -131,7 +131,6 @@ public boolean isSMRTKinetics() { } - public enum ShadeAlignmentsOption { NONE("none"), MAPPING_QUALITY_HIGH("mapping quality high"), @@ -252,9 +251,11 @@ public BisulfiteContext getMatchingBisulfiteContext(final byte[] reference, fina return (matchesContext) ? this : null; } - public String getLabel() { return label; } + public String getLabel() { + return label; + } - BisulfiteContext(String label, byte[] preContext, byte[] postContext){ + BisulfiteContext(String label, byte[] preContext, byte[] postContext) { this.label = label; this.preContext = preContext; this.postContext = postContext; @@ -389,7 +390,7 @@ public void receiveEvent(Object event) { break; } - } else if( event instanceof DataLoadedEvent){ + } else if (event instanceof DataLoadedEvent) { final DataLoadedEvent dataLoaded = (DataLoadedEvent) event; actionToPerformOnFrameLoad.computeIfPresent(dataLoaded.getReferenceFrame(), (k, v) -> { v.accept(k); @@ -747,7 +748,7 @@ public void renderExpandedInsertion(InsertionMarker insertionMarker, RenderConte boolean leaveMargin = getDisplayMode() != DisplayMode.SQUISHED; // Insertion interval - if(this.renderOptions.isShowInsertionMarkers()) { + if (this.renderOptions.isShowInsertionMarkers()) { Graphics2D g = context.getGraphic2DForColor(Color.red); Rectangle iRect = new Rectangle(inputRect.x, insertionRect.y, inputRect.width, insertionRect.height); g.fill(iRect); @@ -830,14 +831,14 @@ private InsertionInterval getInsertionInterval(ReferenceFrame frame, int x, int public void sortRows(final SortOption option, final Double location, final String tag, final boolean invertSort, final Set priorityRecords) { final List frames = FrameManager.getFrames(); for (ReferenceFrame frame : frames) { - Consumer sort = (ReferenceFrame f) -> { + Consumer sort = (ReferenceFrame f) -> { final AlignmentInterval interval = getDataManager().getLoadedInterval(f); final double actloc = location != null ? location : f.getCenter(); interval.sortRows(option, actloc, tag, invertSort, priorityRecords); }; //If the data is loaded sort now, otherwise delay until we get a message that it is loaded. - if(getDataManager().isLoaded(frame)){ - sort.accept(frame); + if (getDataManager().isLoaded(frame)) { + sort.accept(frame); } else { log.debug("Attempt to sort alignments prior to loading"); actionToPerformOnFrameLoad.put(frame, sort); @@ -936,7 +937,6 @@ public String getValueStringAt(String chr, double position, int mouseX, int mous } - Alignment getAlignmentAt(final TrackClickEvent te) { MouseEvent e = te.getMouseEvent(); final ReferenceFrame frame = te.getFrame(); @@ -1331,6 +1331,8 @@ public static class RenderOptions implements Cloneable, Persistable { private Boolean hideSmallIndels; private Integer smallIndelThreshold; + private String basemodFilter; + BisulfiteContext bisulfiteContext = BisulfiteContext.CG; Map peStats; @@ -1601,6 +1603,13 @@ public int getSmallIndelThreshold() { return smallIndelThreshold == null ? getPreferences().getAsInt(SAM_SMALL_INDEL_BP_THRESHOLD) : smallIndelThreshold; } + public String getBasemodFilter() { + return basemodFilter; + } + + public void setBasemodFilter(String basemodFilter) { + this.basemodFilter = basemodFilter; + } @Override public void marshalXML(Document document, Element element) { @@ -1698,6 +1707,9 @@ public void marshalXML(Document document, Element element) { if (showInsertionMarkers != null) { element.setAttribute("showInsertionMarkers", showInsertionMarkers.toString()); } + if (basemodFilter != null) { + element.setAttribute("basemodfilter", basemodFilter); + } } @@ -1800,6 +1812,10 @@ public void unmarshalXML(Element element, Integer version) { if (element.hasAttribute("showInsertionMarkers")) { showInsertionMarkers = Boolean.parseBoolean(element.getAttribute("showInsertionMarkers")); } + if (element.hasAttribute("basemodfilter")) { + basemodFilter = element.getAttribute("basemodfilter"); + } + } } diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index 79c0b16609..d84666367e 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -12,6 +12,7 @@ import org.broad.igv.logging.LogManager; import org.broad.igv.logging.Logger; import org.broad.igv.prefs.PreferencesManager; +import org.broad.igv.sam.mods.BaseModificationUtils; import org.broad.igv.sashimi.SashimiPlot; import org.broad.igv.tools.PFMExporter; import org.broad.igv.track.SequenceTrack; @@ -142,7 +143,7 @@ class AlignmentTrackMenu extends IGVPopupMenu { // Select alignment items addSeparator(); - addSelectByNameItem(alignmentTrack, e); + addSelectByNameItem(alignmentTrack, e); addClearSelectionsMenuItem(); // Copy items @@ -195,7 +196,7 @@ private void addShowChimericRegions(final AlignmentTrack alignmentTrack, final T add(item); } - private void addShowDiagram(final TrackClickEvent e, final Alignment clickedAlignment){ + private void addShowDiagram(final TrackClickEvent e, final Alignment clickedAlignment) { JMenuItem item = new JMenuItem("Supplementary Reads Diagram"); if (clickedAlignment != null && clickedAlignment.getAttribute(SAMTag.SA.name()) != null) { item.setEnabled(true); @@ -472,8 +473,8 @@ void addGroupMenuItem(final TrackClickEvent te) {//ReferenceFrame frame) { private void groupAlignments(AlignmentTrack.GroupOption option, String tag, Range pos) { - if(alignmentTrack.getPreferences().getAsBoolean(SAM_GROUP_ALL)) { - for(AlignmentTrack t : IGV.getInstance().getAlignmentTracks()) { + if (alignmentTrack.getPreferences().getAsBoolean(SAM_GROUP_ALL)) { + for (AlignmentTrack t : IGV.getInstance().getAlignmentTracks()) { t.groupAlignments(option, tag, pos); } } else { @@ -563,10 +564,18 @@ public void addFilterMenuItem() { } private JRadioButtonMenuItem getColorMenuItem(String label, final AlignmentTrack.ColorOption option) { + return getColorMenuItem(label, option, null); + + } + + private JRadioButtonMenuItem getColorMenuItem(String label, final AlignmentTrack.ColorOption option, String extra) { JRadioButtonMenuItem mi = new JRadioButtonMenuItem(label); mi.setSelected(renderOptions.getColorOption() == option); mi.addActionListener(aEvt -> { alignmentTrack.setColorOption(option); + if (option == AlignmentTrack.ColorOption.BASE_MODIFICATION) { + renderOptions.setBasemodFilter(extra); + } alignmentTrack.repaint(); }); @@ -632,18 +641,42 @@ void addColorByMenuItem() { colorMenu.add(getBisulfiteContextMenuItem(group)); // Base modifications - mappings.clear(); - mappings.put("base modification", AlignmentTrack.ColorOption.BASE_MODIFICATION); - mappings.put("base modification (5mC)", AlignmentTrack.ColorOption.BASE_MODIFICATION_5MC); - mappings.put("base modification (all C)", AlignmentTrack.ColorOption.BASE_MODIFICATION_C); - mappings.put("base modification (6mA)", AlignmentTrack.ColorOption.BASE_MODIFICATION_6MA); + JRadioButtonMenuItem bmMenuItem; + colorMenu.addSeparator(); - for (Map.Entry el : mappings.entrySet()) { - JRadioButtonMenuItem mi = getColorMenuItem(el.getKey(), el.getValue()); - colorMenu.add(mi); - group.add(mi); + + Set allModifications = dataManager.getAllBaseModifications(); + + bmMenuItem = getColorMenuItem("CpG modification (5mC)", AlignmentTrack.ColorOption.BASE_MODIFICATION_5MC); + bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION_5MC); + bmMenuItem.setEnabled(allModifications.contains("m")); + colorMenu.add(bmMenuItem); + group.add(bmMenuItem); + + bmMenuItem = getColorMenuItem("CpG modification (all C)", AlignmentTrack.ColorOption.BASE_MODIFICATION_C); + bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION_C); + bmMenuItem.setEnabled(allModifications.contains("m") || allModifications.contains("h")); + colorMenu.add(bmMenuItem); + group.add(bmMenuItem); + + colorMenu.addSeparator(); + + bmMenuItem = getColorMenuItem("base modification (all)", AlignmentTrack.ColorOption.BASE_MODIFICATION); + bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION && renderOptions.getBasemodFilter() == null); + bmMenuItem.setEnabled(!allModifications.isEmpty()); + colorMenu.add(bmMenuItem); + group.add(bmMenuItem); + + for(String m : allModifications) { + String name = BaseModificationUtils.modificationName(m); + bmMenuItem = getColorMenuItem("base modification (" + name + ")", AlignmentTrack.ColorOption.BASE_MODIFICATION, m); + bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION && m.equals(renderOptions.getBasemodFilter())); + colorMenu.add(bmMenuItem); + group.add(bmMenuItem); } + + // SMRT kinetics if (alignmentTrack.getPreferences().getAsBoolean(SMRT_KINETICS_SHOW_OPTIONS)) { // Show additional options to help visualize SMRT kinetics data mappings.clear(); @@ -652,7 +685,7 @@ void addColorByMenuItem() { mappings.put("SMRT CCS fwd-strand aligned IPD", AlignmentTrack.ColorOption.SMRT_CCS_FWD_IPD); mappings.put("SMRT CCS fwd-strand aligned PW", AlignmentTrack.ColorOption.SMRT_CCS_FWD_PW); mappings.put("SMRT CCS rev-strand aligned IPD", AlignmentTrack.ColorOption.SMRT_CCS_REV_IPD); - mappings.put("SMRT CCS rev-strand aligned PW",AlignmentTrack.ColorOption.SMRT_CCS_REV_PW); + mappings.put("SMRT CCS rev-strand aligned PW", AlignmentTrack.ColorOption.SMRT_CCS_REV_PW); colorMenu.addSeparator(); for (Map.Entry el : mappings.entrySet()) { JRadioButtonMenuItem mi = getColorMenuItem(el.getKey(), el.getValue()); diff --git a/src/main/java/org/broad/igv/sam/CoverageTrack.java b/src/main/java/org/broad/igv/sam/CoverageTrack.java index 5a0730d24f..d386f6a2e0 100644 --- a/src/main/java/org/broad/igv/sam/CoverageTrack.java +++ b/src/main/java/org/broad/igv/sam/CoverageTrack.java @@ -531,7 +531,8 @@ private void paint(final RenderContext context, final Rectangle rect, final Alig drawBarBisulfite(context, pX, bottomY, dX, barHeight, totalCount, bc); } } else if (colorOption.isBaseMod()) { - BaseModificationCoverageRenderer.drawModifications(context, pX, bottomY, dX, barHeight, pos, alignmentCounts, colorOption); + String basemodFilter = alignmentTrack != null ? alignmentTrack.getRenderOptions().getBasemodFilter() : null; + BaseModificationCoverageRenderer.drawModifications(context, pX, bottomY, dX, barHeight, pos, alignmentCounts, colorOption, basemodFilter); } else { if (refBases != null) { int refIdx = pos - intervalStart; diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java index 5c0a73596e..e0e137d0e5 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java @@ -18,7 +18,8 @@ public static void drawModifications(RenderContext context, int barHeight, int pos, AlignmentCounts alignmentCounts, - ColorOption colorOption) { + ColorOption colorOption, + String basemodFilter) { switch (colorOption) { case BASE_MODIFICATION_5MC: @@ -31,7 +32,7 @@ public static void drawModifications(RenderContext context, draw(context, pX, pBottom, dX, barHeight, pos, alignmentCounts, "a"); break; default: - draw(context, pX, pBottom, dX, barHeight, pos, alignmentCounts, null); + draw(context, pX, pBottom, dX, barHeight, pos, alignmentCounts, basemodFilter); } } diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java index d0d2651571..8f07af07c7 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java @@ -15,7 +15,8 @@ public static void drawModifications( double locScale, Rectangle rowRect, Graphics g, - AlignmentTrack.ColorOption colorOption) { + AlignmentTrack.ColorOption colorOption, + String basemodFilter) { switch (colorOption) { case BASE_MODIFICATION_5MC: @@ -28,7 +29,7 @@ public static void drawModifications( draw(alignment, bpStart, locScale, rowRect, g, "a"); break; default: - draw(alignment, bpStart, locScale, rowRect, g, null); + draw(alignment, bpStart, locScale, rowRect, g, basemodFilter); } } diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java index 1f24423253..5912a4190d 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java @@ -40,8 +40,12 @@ public class BaseModificationUtils { public static String valueString(String modification, byte likelihood) { int l = (int) (100.0 * Byte.toUnsignedInt(likelihood) / 255); - return "Base modification: " + - ((codeValues.containsKey(modification)) ? codeValues.get(modification) : "Uknown") + " (" + l + "%)"; + return "Base modification: " + modificationName(modification) + " (" + l + "%)"; + } + + + public static String modificationName(String modification) { + return ((codeValues.containsKey(modification)) ? codeValues.get(modification) : modification); } From cb0bb8180445e90a26486fd1c42125efda31c7ec Mon Sep 17 00:00:00 2001 From: Alexandr Chernov Date: Wed, 19 Jul 2023 07:19:39 +0200 Subject: [PATCH 071/116] Set focus on OK button in LoginDialog (#1362) --- src/main/java/org/broad/igv/util/LoginDialog.java | 1 + 1 file changed, 1 insertion(+) diff --git a/src/main/java/org/broad/igv/util/LoginDialog.java b/src/main/java/org/broad/igv/util/LoginDialog.java index e99d2a271c..8ed8546fab 100644 --- a/src/main/java/org/broad/igv/util/LoginDialog.java +++ b/src/main/java/org/broad/igv/util/LoginDialog.java @@ -165,6 +165,7 @@ public void actionPerformed(ActionEvent e) { okButtonActionPerformed(e); } }); + this.getRootPane().setDefaultButton(okButton); buttonBar.add(okButton, new GridBagConstraints(1, 0, 1, 1, 0.0, 0.0, GridBagConstraints.CENTER, GridBagConstraints.BOTH, new Insets(0, 0, 0, 5), 0, 0)); From 5b8090a0a6e27f536ab05fbfccdb09153df1f0b2 Mon Sep 17 00:00:00 2001 From: Alexandr Chernov Date: Wed, 19 Jul 2023 19:59:50 +0200 Subject: [PATCH 072/116] Added a port command for getting a full path of a currently loaded genome (#1360) --- .../org/broad/igv/batch/CommandExecutor.java | 16 ++++++++++++++++ .../igv/feature/genome/load/GenomeLoader.java | 2 +- 2 files changed, 17 insertions(+), 1 deletion(-) diff --git a/src/main/java/org/broad/igv/batch/CommandExecutor.java b/src/main/java/org/broad/igv/batch/CommandExecutor.java index d941715fdc..da096b261c 100755 --- a/src/main/java/org/broad/igv/batch/CommandExecutor.java +++ b/src/main/java/org/broad/igv/batch/CommandExecutor.java @@ -35,6 +35,7 @@ import org.broad.igv.feature.Range; import org.broad.igv.feature.RegionOfInterest; import org.broad.igv.feature.Strand; +import org.broad.igv.feature.genome.GenomeListItem; import org.broad.igv.feature.genome.GenomeManager; import org.broad.igv.logging.LogManager; import org.broad.igv.logging.Logger; @@ -50,6 +51,7 @@ import org.broad.igv.ui.IGV; import org.broad.igv.ui.action.OverlayTracksMenuAction; import org.broad.igv.ui.color.ColorUtilities; +import org.broad.igv.ui.commandbar.GenomeListManager; import org.broad.igv.ui.panel.FrameManager; import org.broad.igv.ui.util.MessageUtils; import org.broad.igv.ui.util.SnapshotUtilities; @@ -135,6 +137,20 @@ public String execute(String commandLine) { result = load(param1, args.size() > 2 ? args.subList(2, args.size()) : null); } else if (cmd.equalsIgnoreCase("genome") && args.size() > 1) { result = genome(param1); + } else if (cmd.equalsIgnoreCase("currentGenomePath")) { + result = ""; + if (GenomeManager.getInstance().getCurrentGenome() == null) { + return result; + } + String id = GenomeManager.getInstance().getCurrentGenome().getId(); + if (id != null) + { + GenomeListItem item = GenomeListManager.getInstance().getGenomeListItem(id); + if (item != null) { + result = item.getPath(); + } + } + return result; } else if (cmd.equalsIgnoreCase("new") || cmd.equalsIgnoreCase("reset") || cmd.equalsIgnoreCase("clear")) { igv.newSession(); } else if (cmd.equalsIgnoreCase("region")) { diff --git a/src/main/java/org/broad/igv/feature/genome/load/GenomeLoader.java b/src/main/java/org/broad/igv/feature/genome/load/GenomeLoader.java index e4cb9178ec..9df2b5d7f7 100644 --- a/src/main/java/org/broad/igv/feature/genome/load/GenomeLoader.java +++ b/src/main/java/org/broad/igv/feature/genome/load/GenomeLoader.java @@ -71,7 +71,7 @@ public static Collection> loadChrAliases(String path) { return loadChrAliases(br); } catch (IOException e) { log.error("Error loading chr alias table", e); - MessageUtils.showMessage("Error loading chromosome alias table. Aliases will not be avaliable
" + + MessageUtils.showMessage("Error loading chromosome alias table. Aliases will not be available
" + e.toString()); return null; } finally { From de2e510b9030de91ba461495ed896e812db6cc5e Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Tue, 1 Aug 2023 22:22:36 -0700 Subject: [PATCH 073/116] Basemod 3 (#1364) * Base modification visualization redesign * Coverage track reflects counts of reads with modification above a threshold * Options to use 2-color or monocolor schemes for all modifications see #1359 --- .../java/org/broad/igv/prefs/Constants.java | 64 ++++-- .../org/broad/igv/prefs/IGVPreferences.java | 8 + .../org/broad/igv/sam/AlignmentCounts.java | 4 + .../broad/igv/sam/AlignmentDataManager.java | 35 +++- .../org/broad/igv/sam/AlignmentRenderer.java | 6 +- .../org/broad/igv/sam/AlignmentTrack.java | 53 ++++- .../org/broad/igv/sam/AlignmentTrackMenu.java | 66 +++--- .../java/org/broad/igv/sam/CoverageTrack.java | 8 +- .../broad/igv/sam/DenseAlignmentCounts.java | 23 +++ .../broad/igv/sam/ReducedMemoryAlignment.java | 11 +- .../broad/igv/sam/SparseAlignmentCounts.java | 26 +++ .../igv/sam/mods/BaseModficationFilter.java | 87 ++++++++ .../igv/sam/mods/BaseModificationColors.java | 109 ++++------ .../igv/sam/mods/BaseModificationCounts.java | 152 +++++++------- .../BaseModificationCoverageRenderer.java | 194 ++++-------------- .../igv/sam/mods/BaseModificationKey.java | 40 +++- .../sam/mods/BaseModificationRenderer.java | 171 +++------------ .../igv/sam/mods/BaseModificationSet.java | 5 +- .../igv/sam/mods/BaseModificationUtils.java | 3 +- .../igv/util/collections/ByteArrayList.java | 134 ++++++++++++ .../igv/util/collections/IntArrayList.java | 1 - .../org/broad/igv/prefs/preferences.tab | 26 ++- .../sam/mods/BaseModficationFilterTest.java | 32 +++ .../sam/mods/BaseModificationCountsTest.java | 33 ++- .../igv/sam/mods/BaseModificationsTest.java | 1 + 25 files changed, 758 insertions(+), 534 deletions(-) create mode 100644 src/main/java/org/broad/igv/sam/mods/BaseModficationFilter.java create mode 100644 src/main/java/org/broad/igv/util/collections/ByteArrayList.java create mode 100644 src/test/java/org/broad/igv/sam/mods/BaseModficationFilterTest.java diff --git a/src/main/java/org/broad/igv/prefs/Constants.java b/src/main/java/org/broad/igv/prefs/Constants.java index 594678926f..a902105b8b 100644 --- a/src/main/java/org/broad/igv/prefs/Constants.java +++ b/src/main/java/org/broad/igv/prefs/Constants.java @@ -47,7 +47,6 @@ private Constants() { public static final String LAST_GENOME_IMPORT_DIRECTORY = "LAST_GENOME_IMPORT_DIRECTORY"; public static final String DEFINE_GENOME_INPUT_DIRECTORY_KEY = "DEFINE_GENOME_INPUT_DIRECTORY_KEY"; public static final String DEFAULT_GENOME = "DEFAULT_GENOME_KEY"; - public static final String AUTO_UPDATE_GENOMES = "AUTO_UPDATE_GENOMES"; public static final String FRAME_BOUNDS_KEY = "IGV.Bounds"; @@ -112,7 +111,6 @@ private Constants() { public static final String SAM_SHADE_QUALITY_HIGH = "SAM.SHADE_QUALITY_HIGH"; public static final String SAM_SHADE_ALIGNMENT_BY = "SAM.SHADE_ALIGNMENT_BY"; public static final String SAM_ALIGNMENT_SCORE_THRESHOLD = "SAM.ALIGNMENT_SCORE_THRESHOLD"; - public static final String SAM_BASEMOD_THRESHOLD = "SAM.BASEMOD_THRESHOLD"; public static final String SAM_COMPUTE_ISIZES = "SAM.COMPUTE_ISIZES"; public static final String SAM_MAX_INSERT_SIZE_THRESHOLD = "SAM.INSERT_SIZE_THRESHOLD"; public static final String SAM_MIN_INSERT_SIZE_THRESHOLD = "SAM.MIN_INSERT_SIZE_THRESHOLD"; @@ -183,6 +181,25 @@ private Constants() { public static final String SAM_DISPLAY_PAIRED = "SAM.DISPLAY_PAIRED"; public static final String KNOWN_SNPS = "KNOWN_SNPS_FILE"; + + // Base modification settings + public static final String BASEMOD_THRESHOLD = "BASEMOD.THRESHOLD"; + public static final String BASEMOD_M_COLOR = "BASEMOD.M_COLOR"; + public static final String BASEMOD_H_COLOR = "BASEMOD.H_COLOR"; + public static final String BASEMOD_F_COLOR = "BASEMOD.F_COLOR"; + public static final String BASEMOD_C_COLOR = "BASEMOD.C_COLOR"; + public static final String BASEMOD_G_COLOR = "BASEMOD.G_COLOR"; + public static final String BASEMOD_E_COLOR = "BASEMOD.E_COLOR"; + public static final String BASEMOD_B_COLOR = "BASEMOD.B_COLOR"; + public static final String BASEMOD_A_COLOR = "BASEMOD.A_COLOR"; + public static final String BASEMOD_O_COLOR = "BASEMOD.O_COLOR"; + public static final String BASEMOD_OTHER_COLOR = "BASEMOD.OTHER_COLOR"; + + public static final String BASEMOD_NONE_A_COLOR = "BASEMOD.NONE_A_COLOR"; + public static final String BASEMOD_NONE_C_COLOR = "BASEMOD.NONE_C_COLOR"; + public static final String BASEMOD_NONE_T_COLOR = "BASEMOD.NONE_T_COLOR"; + public static final String BASEMOD_NONE_G_COLOR = "BASEMOD.NONE_G_COLOR"; + public static final String BASEMOD_NONE_N_COLOR = "BASEMOD.NONE_N_COLOR"; public static final String SMRT_KINETICS_SHOW_OPTIONS = "SMRT_KINETICS.SHOW_OPTIONS"; // Sequence track settings @@ -242,25 +259,10 @@ private Constants() { public static final String PROBE_MAPPING_FILE = "PROBE_MAPPING_FILE"; public static final String USE_PROBE_MAPPING_FILE = "USE_PROBE_MAPPING_FILE"; - // Genome space - public static final String GENOME_SPACE_ENABLE = "GENOME_SPACE_ENABLE"; - public static final String GENOME_SPACE_DM_SERVER = "GENOME_SPACE_DM_SERVER"; - public static final String GENOME_SPACE_ATM_SERVER = "GENOME_SPACE_ATM_SERVER"; - public static final String GENOME_SPACE_IDENTITY_SERVER = "GENOME_SPACE_IDENTITY_SERVER"; - - // Google - public static final String GOOGLE_API_KEY = "GOOGLE_API_KEY"; public static final String GOOGLE_PROJECT = "GOOGLE_PROJECT"; public static final String ENABLE_GOOGLE_MENU = "ENABLE_GOOGLE_MENU"; public static final String SAVE_GOOGLE_CREDENTIALS = "SAVE_GOOGLE_CREDENTIALS"; - // CBIO connections - public static final String CBIO_MUTATION_THRESHOLD = "CBIO_MUTATION_THRESHOLD"; - public static final String CBIO_AMPLIFICATION_THRESHOLD = "CBIO_AMPLIFICATION_THRESHOLD"; - public static final String CBIO_DELETION_THRESHOLD = "CBIO_DELETION_THRESHOLD"; - public static final String CBIO_EXPRESSION_UP_THRESHOLD = "CBIO_EXPRESSION_UP_THRESHOLD"; - public static final String CBIO_EXPRESSION_DOWN_THRESHOLD = "CBIO_EXPRESSION_DOWN_THRESHOLD"; - // Proxy settings public static final String USE_PROXY = "PROXY.USE"; public static final String PROXY_HOST = "PROXY.HOST"; @@ -289,8 +291,6 @@ private Constants() { public static final String CIRC_VIEW_PORT = "CIRC_VIEW_PORT"; public static final String CIRC_VIEW_HOST = "CIRC_VIEW_HOST"; - // Experimental - public static final String SCORE_VARIANTS = "SCORE_VARIANTS"; /** @@ -312,8 +312,7 @@ private Constants() { SAM_FILTER_SECONDARY_ALIGNMENTS, SAM_FILTER_SUPPLEMENTARY_ALIGNMENTS, SAM_JUNCTION_MIN_FLANKING_WIDTH, - SAM_JUNCTION_MIN_COVERAGE, - SAM_BASEMOD_THRESHOLD + SAM_JUNCTION_MIN_COVERAGE ); /** @@ -330,7 +329,28 @@ private Constants() { SAM_GROUP_BY_TAG, SAM_SHADE_QUALITY_LOW, SAM_SHADE_QUALITY_HIGH, - SAM_SHADE_ALIGNMENT_BY + SAM_SHADE_ALIGNMENT_BY, + BASEMOD_THRESHOLD, + SMRT_KINETICS_SHOW_OPTIONS + ); + + static java.util.List BASEMOD_COLOR_KEYS = Arrays.asList( + + BASEMOD_M_COLOR, + BASEMOD_H_COLOR, + BASEMOD_F_COLOR, + BASEMOD_C_COLOR, + BASEMOD_G_COLOR, + BASEMOD_E_COLOR, + BASEMOD_B_COLOR, + BASEMOD_A_COLOR, + BASEMOD_O_COLOR, + BASEMOD_OTHER_COLOR, + BASEMOD_NONE_A_COLOR, + BASEMOD_NONE_C_COLOR, + BASEMOD_NONE_T_COLOR, + BASEMOD_NONE_G_COLOR, + BASEMOD_NONE_N_COLOR ); /** diff --git a/src/main/java/org/broad/igv/prefs/IGVPreferences.java b/src/main/java/org/broad/igv/prefs/IGVPreferences.java index 325bff1ab2..77846c6c82 100644 --- a/src/main/java/org/broad/igv/prefs/IGVPreferences.java +++ b/src/main/java/org/broad/igv/prefs/IGVPreferences.java @@ -40,6 +40,7 @@ import org.broad.igv.logging.Logger; import org.broad.igv.renderer.ColorScaleFactory; import org.broad.igv.renderer.ContinuousColorScale; +import org.broad.igv.sam.mods.BaseModificationColors; import org.broad.igv.track.TrackType; import org.broad.igv.ui.IGV; import org.broad.igv.ui.UIConstants; @@ -329,6 +330,13 @@ private void checkForAlignmentChanges(Map updatedPreferenceMap) break; } } + for (String key : BASEMOD_COLOR_KEYS) { + if (updatedPreferenceMap.containsKey(key)) { + refreshSAM = true; + BaseModificationColors.updateColors(); + break; + } + } if (reloadSAM) { IGVEventBus.getInstance().post(new AlignmentTrackEvent(this, AlignmentTrackEvent.Type.RELOAD)); diff --git a/src/main/java/org/broad/igv/sam/AlignmentCounts.java b/src/main/java/org/broad/igv/sam/AlignmentCounts.java index 98ce5a69a2..f0079e7895 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentCounts.java +++ b/src/main/java/org/broad/igv/sam/AlignmentCounts.java @@ -38,6 +38,10 @@ public interface AlignmentCounts extends Feature { int getTotalCount(int pos); + public int getTotalPositiveCount(int pos); + + public int getTotalNegativeCount(int pos); + int getTotalQuality(int pos); int getCount(int pos, byte b); diff --git a/src/main/java/org/broad/igv/sam/AlignmentDataManager.java b/src/main/java/org/broad/igv/sam/AlignmentDataManager.java index 6412852b53..cb50c3ace5 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentDataManager.java +++ b/src/main/java/org/broad/igv/sam/AlignmentDataManager.java @@ -36,6 +36,7 @@ import org.broad.igv.feature.genome.Genome; import org.broad.igv.prefs.IGVPreferences; import org.broad.igv.prefs.PreferencesManager; +import org.broad.igv.sam.mods.BaseModificationKey; import org.broad.igv.sam.mods.BaseModificationSet; import org.broad.igv.sam.reader.AlignmentReader; import org.broad.igv.sam.reader.AlignmentReaderFactory; @@ -69,7 +70,8 @@ public class AlignmentDataManager implements IGVEventObserver { private Map peStats; private SpliceJunctionHelper.LoadOptions loadOptions; - private Set allBaseModifications = new HashSet<>(); + private Set allBaseModificationKeys = new HashSet<>(); + private Set simplexBaseModfications = new HashSet<>(); private Range currentlyLoading; @@ -198,8 +200,10 @@ public Map getPEStats() { /** * Return all base modfications seen in loaded alignments */ - public Set getAllBaseModifications() { - return allBaseModifications; + + public Set getAllBaseModificationKeys() { + return allBaseModificationKeys; + } public boolean isPairedEnd() { @@ -407,14 +411,25 @@ AlignmentInterval loadInterval(String chr, int start, int end, AlignmentTrack.Re } private void updateBaseModfications(List alignments) { - for(Alignment a : alignments) { - List bmSets = a.getBaseModificationSets(); - if(bmSets != null) { - for(BaseModificationSet bms : bmSets) { - allBaseModifications.add(bms.getModification()); + + allBaseModificationKeys.add(BaseModificationKey.getKey(bms.getBase(), bms.getStrand(), bms.getModification())); } } } + // Search for simplex modifications (single strand read, e.g. C+m with no G-m) + Map tmp = new HashMap<>(); + for(BaseModificationKey key : allBaseModificationKeys) { + if(tmp.containsKey(key.getModification())) { + tmp.remove(key); + } else { + tmp.put(key.getModification(), key); + } + } + for(Map.Entry entries : tmp.entrySet()) { + simplexBaseModfications.add(entries.getKey()); + simplexBaseModfications.add("NONE_" + entries.getValue().getCanonicalBase()); + } + } public AlignmentTrack.ExperimentType inferType() { @@ -573,6 +588,10 @@ public Collection getLoadedIntervals() { return intervalCache; } + public Set getSimplexBaseModifications() { + return simplexBaseModfications; + } + public static class DownsampleOptions { private boolean downsample; private int sampleWindowSize; diff --git a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java index b09f76004d..b7927f0853 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java +++ b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java @@ -853,7 +853,7 @@ private void drawAlignment( // Base modification if (colorOption.isBaseMod()) { - BaseModificationRenderer.drawModifications(alignment, bpStart, locScale, rowRect, context.getGraphics(), colorOption, renderOptions.getBasemodFilter()); + BaseModificationRenderer.drawModifications(alignment, bpStart, locScale, rowRect, context.getGraphics(), colorOption, renderOptions.getBasemodFilter(), renderOptions.getBasemodThreshold()); } // Kinetic data @@ -1299,9 +1299,7 @@ private Color getAlignmentColor(Alignment alignment, AlignmentTrack track) { case BISULFITE: case BASE_MODIFICATION: - case BASE_MODIFICATION_5MC: - case BASE_MODIFICATION_C: - case BASE_MODIFICATION_6MA: + case BASE_MODIFICATION_2COLOR: case SMRT_SUBREAD_IPD: case SMRT_SUBREAD_PW: case SMRT_CCS_FWD_IPD: diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrack.java b/src/main/java/org/broad/igv/sam/AlignmentTrack.java index 0cb4c7222d..1bed4a314d 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrack.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrack.java @@ -41,6 +41,7 @@ import org.broad.igv.prefs.IGVPreferences; import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.renderer.GraphicUtils; +import org.broad.igv.sam.mods.BaseModficationFilter; import org.broad.igv.session.Persistable; import org.broad.igv.track.*; import org.broad.igv.ui.FontManager; @@ -101,9 +102,7 @@ public enum ColorOption { LINK_STRAND, YC_TAG, BASE_MODIFICATION, - BASE_MODIFICATION_5MC, - BASE_MODIFICATION_C, - BASE_MODIFICATION_6MA, + BASE_MODIFICATION_2COLOR, SMRT_SUBREAD_IPD, SMRT_SUBREAD_PW, SMRT_CCS_FWD_IPD, @@ -112,7 +111,7 @@ public enum ColorOption { SMRT_CCS_REV_PW; public boolean isBaseMod() { - return this == BASE_MODIFICATION || this == BASE_MODIFICATION_5MC || this == BASE_MODIFICATION_C || this == BASE_MODIFICATION_6MA; + return this == BASE_MODIFICATION || this == BASE_MODIFICATION_2COLOR; } public boolean isSMRTKinetics() { @@ -1331,7 +1330,10 @@ public static class RenderOptions implements Cloneable, Persistable { private Boolean hideSmallIndels; private Integer smallIndelThreshold; - private String basemodFilter; + private BaseModficationFilter basemodFilter; + + private Float basemodThreshold; + BisulfiteContext bisulfiteContext = BisulfiteContext.CG; Map peStats; @@ -1603,11 +1605,20 @@ public int getSmallIndelThreshold() { return smallIndelThreshold == null ? getPreferences().getAsInt(SAM_SMALL_INDEL_BP_THRESHOLD) : smallIndelThreshold; } - public String getBasemodFilter() { + public BaseModficationFilter getBasemodFilter() { return basemodFilter; } - public void setBasemodFilter(String basemodFilter) { + public float getBasemodThreshold() { + return basemodThreshold == null ? getPreferences().getAsFloat(BASEMOD_THRESHOLD) : basemodThreshold.floatValue(); + } + + public void setBasemodThreshold(float basemodThreshold) { + this.basemodThreshold = basemodThreshold; + } + + public void setBasemodFilter(BaseModficationFilter basemodFilter) { + this.basemodFilter = basemodFilter; } @@ -1708,7 +1719,11 @@ public void marshalXML(Document document, Element element) { element.setAttribute("showInsertionMarkers", showInsertionMarkers.toString()); } if (basemodFilter != null) { - element.setAttribute("basemodfilter", basemodFilter); + element.setAttribute("basemodFilter", basemodFilter.toString()); + } + if(basemodThreshold != null) { + element.setAttribute("basemodThredhold", String.valueOf(basemodThreshold)); + } } @@ -1738,7 +1753,20 @@ public void unmarshalXML(Element element, Integer version) { maxInsertSize = Integer.parseInt(element.getAttribute("maxInsertSize")); } if (element.hasAttribute("colorOption")) { - colorOption = ColorOption.valueOf(element.getAttribute("colorOption")); + // Convert deprecated options + final String attributeValue = element.getAttribute("colorOption"); + if("BASE_MODIFICATION_6MA".equals(attributeValue)) { + colorOption = ColorOption.BASE_MODIFICATION; + basemodFilter = new BaseModficationFilter("a"); + } else if("BASE_MODIFICATION_5MC".equals(attributeValue)) { + colorOption = ColorOption.BASE_MODIFICATION_2COLOR; + basemodFilter = new BaseModficationFilter(null, 'C'); + } else if("BASE_MODIFICATION_C".equals(attributeValue)) { + colorOption = ColorOption.BASE_MODIFICATION; + basemodFilter = new BaseModficationFilter(null, 'C'); + }else { + colorOption = ColorOption.valueOf(attributeValue); + } } if (element.hasAttribute("sortOption")) { sortOption = SortOption.valueOf((element.getAttribute("sortOption"))); @@ -1812,8 +1840,11 @@ public void unmarshalXML(Element element, Integer version) { if (element.hasAttribute("showInsertionMarkers")) { showInsertionMarkers = Boolean.parseBoolean(element.getAttribute("showInsertionMarkers")); } - if (element.hasAttribute("basemodfilter")) { - basemodFilter = element.getAttribute("basemodfilter"); + if (element.hasAttribute("basemodFilter")) { + basemodFilter = BaseModficationFilter.fromString(element.getAttribute("basemodfilter")); + } + if (element.hasAttribute("basemodThreshold")) { + basemodFilter = BaseModficationFilter.fromString(element.getAttribute("basemodThreshold")); } } diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index d84666367e..028cfb1ac2 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -1,6 +1,5 @@ package org.broad.igv.sam; -import com.google.gson.annotations.Since; import htsjdk.samtools.SAMTag; import htsjdk.samtools.util.Locatable; import org.broad.igv.Globals; @@ -12,6 +11,7 @@ import org.broad.igv.logging.LogManager; import org.broad.igv.logging.Logger; import org.broad.igv.prefs.PreferencesManager; +import org.broad.igv.sam.mods.BaseModficationFilter; import org.broad.igv.sam.mods.BaseModificationUtils; import org.broad.igv.sashimi.SashimiPlot; import org.broad.igv.tools.PFMExporter; @@ -40,8 +40,6 @@ import java.awt.event.MouseEvent; import java.util.*; import java.util.List; -import java.util.concurrent.CompletableFuture; -import java.util.concurrent.Future; import java.util.function.BiConsumer; import java.util.stream.Collectors; @@ -573,9 +571,7 @@ private JRadioButtonMenuItem getColorMenuItem(String label, final AlignmentTrack mi.setSelected(renderOptions.getColorOption() == option); mi.addActionListener(aEvt -> { alignmentTrack.setColorOption(option); - if (option == AlignmentTrack.ColorOption.BASE_MODIFICATION) { - renderOptions.setBasemodFilter(extra); - } + renderOptions.setBasemodFilter(extra == null ? null : new BaseModficationFilter(extra)); alignmentTrack.repaint(); }); @@ -643,36 +639,44 @@ void addColorByMenuItem() { // Base modifications JRadioButtonMenuItem bmMenuItem; - colorMenu.addSeparator(); - - Set allModifications = dataManager.getAllBaseModifications(); + Set allModifications = dataManager.getAllBaseModificationKeys().stream().map(bmKey -> bmKey.getModification()).collect(Collectors.toSet()); + if (allModifications.size() > 0) { + BaseModficationFilter filter = renderOptions.getBasemodFilter(); - bmMenuItem = getColorMenuItem("CpG modification (5mC)", AlignmentTrack.ColorOption.BASE_MODIFICATION_5MC); - bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION_5MC); - bmMenuItem.setEnabled(allModifications.contains("m")); - colorMenu.add(bmMenuItem); - group.add(bmMenuItem); + colorMenu.addSeparator(); + if (allModifications.size() > 1) { + bmMenuItem = getColorMenuItem("base modification 2-color (all)", AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR); + bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR && filter == null); + colorMenu.add(bmMenuItem); + group.add(bmMenuItem); + } - bmMenuItem = getColorMenuItem("CpG modification (all C)", AlignmentTrack.ColorOption.BASE_MODIFICATION_C); - bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION_C); - bmMenuItem.setEnabled(allModifications.contains("m") || allModifications.contains("h")); - colorMenu.add(bmMenuItem); - group.add(bmMenuItem); + for (String m : allModifications) { + String name = BaseModificationUtils.modificationName(m); + bmMenuItem = getColorMenuItem("base modification 2-color (" + name + ")", AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR, m); + bmMenuItem.setSelected(renderOptions.getColorOption() == + AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR && + (filter != null && filter.pass(m))); + colorMenu.add(bmMenuItem); + group.add(bmMenuItem); + } - colorMenu.addSeparator(); + colorMenu.addSeparator(); + if (allModifications.size() > 1) { + bmMenuItem = getColorMenuItem("base modification (all)", AlignmentTrack.ColorOption.BASE_MODIFICATION); + bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION && filter == null); + colorMenu.add(bmMenuItem); + group.add(bmMenuItem); + } - bmMenuItem = getColorMenuItem("base modification (all)", AlignmentTrack.ColorOption.BASE_MODIFICATION); - bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION && renderOptions.getBasemodFilter() == null); - bmMenuItem.setEnabled(!allModifications.isEmpty()); - colorMenu.add(bmMenuItem); - group.add(bmMenuItem); + for (String m : allModifications) { + String name = BaseModificationUtils.modificationName(m); + bmMenuItem = getColorMenuItem("base modification (" + name + ")", AlignmentTrack.ColorOption.BASE_MODIFICATION, m); + bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION && (filter != null && filter.pass(m))); + colorMenu.add(bmMenuItem); + group.add(bmMenuItem); + } - for(String m : allModifications) { - String name = BaseModificationUtils.modificationName(m); - bmMenuItem = getColorMenuItem("base modification (" + name + ")", AlignmentTrack.ColorOption.BASE_MODIFICATION, m); - bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION && m.equals(renderOptions.getBasemodFilter())); - colorMenu.add(bmMenuItem); - group.add(bmMenuItem); } diff --git a/src/main/java/org/broad/igv/sam/CoverageTrack.java b/src/main/java/org/broad/igv/sam/CoverageTrack.java index d386f6a2e0..c3c70043a3 100644 --- a/src/main/java/org/broad/igv/sam/CoverageTrack.java +++ b/src/main/java/org/broad/igv/sam/CoverageTrack.java @@ -39,6 +39,7 @@ import org.broad.igv.prefs.IGVPreferences; import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.renderer.*; +import org.broad.igv.sam.mods.BaseModficationFilter; import org.broad.igv.sam.mods.BaseModificationCoverageRenderer; import org.broad.igv.tdf.TDFDataSource; import org.broad.igv.tdf.TDFReader; @@ -505,6 +506,7 @@ private void paint(final RenderContext context, final Rectangle rect, final Alig } // Second pass -- potentially overlay mismatches + Set simplexModifications = dataManager.getSimplexBaseModifications(); for (int idx = 0; idx < nPoints; idx++) { int pos = isSparse ? ((SparseAlignmentCounts) alignmentCounts).getPosition(idx) : start + idx * step; @@ -531,8 +533,10 @@ private void paint(final RenderContext context, final Rectangle rect, final Alig drawBarBisulfite(context, pX, bottomY, dX, barHeight, totalCount, bc); } } else if (colorOption.isBaseMod()) { - String basemodFilter = alignmentTrack != null ? alignmentTrack.getRenderOptions().getBasemodFilter() : null; - BaseModificationCoverageRenderer.drawModifications(context, pX, bottomY, dX, barHeight, pos, alignmentCounts, colorOption, basemodFilter); + BaseModficationFilter basemodFilter = alignmentTrack != null ? alignmentTrack.getRenderOptions().getBasemodFilter() : null; + float threshold = alignmentTrack.getRenderOptions().getBasemodThreshold(); + BaseModificationCoverageRenderer.drawModifications(context, pX, bottomY, dX, barHeight, pos, alignmentCounts, colorOption, basemodFilter, threshold, simplexModifications); + } else { if (refBases != null) { int refIdx = pos - intervalStart; diff --git a/src/main/java/org/broad/igv/sam/DenseAlignmentCounts.java b/src/main/java/org/broad/igv/sam/DenseAlignmentCounts.java index fefd7a6a37..4aeb31a23d 100644 --- a/src/main/java/org/broad/igv/sam/DenseAlignmentCounts.java +++ b/src/main/java/org/broad/igv/sam/DenseAlignmentCounts.java @@ -134,7 +134,30 @@ public int getTotalCount(int pos) { return 0; } else { return posTotal[offset] + negTotal[offset]; + } + } + public int getTotalPositiveCount(int pos) { + int offset = pos - start; + if (offset < 0 || offset >= posA.length) { + if (log.isDebugEnabled()) { + log.debug("Position out of range: " + pos + " (valid range - " + start + "-" + end); + } + return 0; + } else { + return posTotal[offset]; + } + } + + public int getTotalNegativeCount(int pos) { + int offset = pos - start; + if (offset < 0 || offset >= posA.length) { + if (log.isDebugEnabled()) { + log.debug("Position out of range: " + pos + " (valid range - " + start + "-" + end); + } + return 0; + } else { + return negTotal[offset]; } } diff --git a/src/main/java/org/broad/igv/sam/ReducedMemoryAlignment.java b/src/main/java/org/broad/igv/sam/ReducedMemoryAlignment.java index 90fe24cf65..24421b73d9 100644 --- a/src/main/java/org/broad/igv/sam/ReducedMemoryAlignment.java +++ b/src/main/java/org/broad/igv/sam/ReducedMemoryAlignment.java @@ -468,10 +468,19 @@ public int getTotalCount(int pos) { return 0; } else { return (int) Math.round(total[offset]); - } } + @Override + public int getTotalPositiveCount(int pos) { + throw new RuntimeException(" Method getTotalPositiveCount not implemented"); + } + + @Override + public int getTotalNegativeCount(int pos) { + throw new RuntimeException(" Method getTotalNegativeCount not implemented"); + } + @Override public void incCounts(Alignment alignment) { diff --git a/src/main/java/org/broad/igv/sam/SparseAlignmentCounts.java b/src/main/java/org/broad/igv/sam/SparseAlignmentCounts.java index d2852f0eaf..02de430601 100644 --- a/src/main/java/org/broad/igv/sam/SparseAlignmentCounts.java +++ b/src/main/java/org/broad/igv/sam/SparseAlignmentCounts.java @@ -144,6 +144,32 @@ public int getTotalCount(int pos) { } } + @Override + public int getTotalPositiveCount(int pos) { + if (!indexMap.containsKey(pos)) { + if (log.isDebugEnabled()) { + log.debug("Position out of range: " + pos + " (valid range - " + start + "-" + end); + } + return 0; + } else { + int idx = getIndex(pos); + return getCountFromList(posTotal, idx); + } + } + + @Override + public int getTotalNegativeCount(int pos) { + if (!indexMap.containsKey(pos)) { + if (log.isDebugEnabled()) { + log.debug("Position out of range: " + pos + " (valid range - " + start + "-" + end); + } + return 0; + } else { + int idx = getIndex(pos); + return getCountFromList(negTotal, idx); + + } + } public int getTotalQuality(int pos) { if (!indexMap.containsKey(pos)) { diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModficationFilter.java b/src/main/java/org/broad/igv/sam/mods/BaseModficationFilter.java new file mode 100644 index 0000000000..999501a8ca --- /dev/null +++ b/src/main/java/org/broad/igv/sam/mods/BaseModficationFilter.java @@ -0,0 +1,87 @@ +package org.broad.igv.sam.mods; + +import java.util.Map; +import java.util.stream.Collectors; +import java.util.stream.Stream; + +public class BaseModficationFilter { + + String modification; + char base = 0; + + public BaseModficationFilter(String modification, char base) { + this.modification = modification; + this.base = base; + } + + public BaseModficationFilter(String modification) { + this.modification = modification; + this.base = 0; + } + + public boolean pass(String mod) { + if(mod.startsWith("NONE_")) { + char b = mod.charAt(5); + return (this.base > 0 && this.base == b) || + (modToBaseMap.containsKey(this.modification.substring(0, 1)) && + modToBaseMap.get(this.modification.substring(0, 1)).charAt(0) == b); + } else { + return (modification == null || modification.contains(mod)); + } + } + + public boolean pass(String mod, char b) { + + if(this.base != 0 && this.base == b) { + return true; + } else { + return pass(mod); + } + } + + @Override + public String toString() { + return (modification == null ? "" : modification) + "," + (base == 0 ? "" : base); + } + + public static BaseModficationFilter fromString(String str) { + int idx = str.indexOf(","); + String mod; + char base; + if (idx < 0) { + // Backward compatibility + mod = str; + base = 0; + } else if (idx == 0) { + mod = null; + base = str.charAt(1); + } else { + mod = str.substring(0, idx); + base = idx == str.length() - 1 ? 0 : str.charAt(idx + 1); + } + return new BaseModficationFilter(mod, base); + + } + + public static Map modToBaseMap; + static { + modToBaseMap = Stream.of(new String[][]{ + {"m", "C"}, + {"h", "C"}, + {"f", "C"}, + {"c", "C"}, + {"C", "C"}, + {"g", "T"}, + {"e", "T"}, + {"b", "T"}, + {"T", "T"}, + {"a", "A"}, + {"A", "A"}, + {"o", "G"}, + {"G", "G"}, + {"n", "N"}, + {"N", "N"} + }).collect(Collectors.toMap(data -> data[0], data -> data[1])); + + } +} diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationColors.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationColors.java index ea2ddecc47..8044199650 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationColors.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationColors.java @@ -2,9 +2,11 @@ import org.broad.igv.logging.LogManager; import org.broad.igv.logging.Logger; +import org.broad.igv.prefs.Constants; +import org.broad.igv.prefs.IGVPreferences; import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.sam.AlignmentTrack; - +import static org.broad.igv.prefs.Constants.*; import java.awt.*; import java.util.HashMap; import java.util.Map; @@ -23,103 +25,62 @@ public class BaseModificationColors { private static Logger log = LogManager.getLogger(BaseModificationColors.class); - static Color genericColor = new Color(132, 178, 158); - public static Color noModColor5MC = Color.blue; - - public static Color color6ma = new Color(51, 0, 111); static HashMap colors = new HashMap<>(); // 5MC overrides -- avoid colors close to blue for "C" modificatoins static HashMap colors5MC = new HashMap<>(); - static { - colors.put("m", Color.red); - colors.put("h", new Color(11, 132, 165)); - colors.put("o", new Color(111, 78, 129)); - colors.put("f", new Color(246, 200, 95)); - colors.put("c", new Color(157, 216, 102)); - colors.put("g", new Color(255, 160, 86)); - colors.put("e", new Color(141, 221, 208)); - colors.put("b", new Color(202, 71, 47)); - colors.put("a", new Color(51, 0, 111)); - colors5MC.put("h", new Color(255, 0, 255)); + static Color genericColor = new Color(132, 178, 158); + + public static void updateColors () { + IGVPreferences preferences = PreferencesManager.getPreferences(); + colors.put("m", preferences.getAsColor(BASEMOD_M_COLOR)); + colors.put("h", preferences.getAsColor(BASEMOD_H_COLOR)); + colors.put("o", preferences.getAsColor(BASEMOD_O_COLOR)); + colors.put("f", preferences.getAsColor(BASEMOD_F_COLOR)); + colors.put("c", preferences.getAsColor(BASEMOD_C_COLOR)); + colors.put("g", preferences.getAsColor(BASEMOD_G_COLOR)); + colors.put("e", preferences.getAsColor(BASEMOD_E_COLOR)); + colors.put("b", preferences.getAsColor(BASEMOD_B_COLOR)); + colors.put("a", preferences.getAsColor(BASEMOD_A_COLOR)); + colors.put("other", preferences.getAsColor(BASEMOD_OTHER_COLOR)); + colors.put("NONE_A", preferences.getAsColor(BASEMOD_NONE_A_COLOR)); + colors.put("NONE_C", preferences.getAsColor(BASEMOD_NONE_C_COLOR)); + colors.put("NONE_U", preferences.getAsColor(BASEMOD_NONE_C_COLOR)); + colors.put("NONE_T", preferences.getAsColor(BASEMOD_NONE_T_COLOR)); + colors.put("NONE_G", preferences.getAsColor(BASEMOD_NONE_G_COLOR)); + colors.put("NONE_N", preferences.getAsColor(BASEMOD_NONE_N_COLOR)); } /** * Cache for modified colors */ static Map modColorMap = new HashMap<>(); - static Map modColorMap5MC = new HashMap<>(); - - - public static Color getModColor(String modification, byte likelihood, AlignmentTrack.ColorOption colorOption) { - - // Note the pallete will always return a color, either an initially seeded one if supplied or a random color. - Color baseColor = getBaseColor(modification, colorOption); - - int l = Byte.toUnsignedInt(likelihood); - if (l > 255) { - return baseColor; - } - - String key = modification + "--" + l; - if (colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_5MC || - colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_C) { - if (!modColorMap5MC.containsKey(key)) { - int alpha = Math.min(255, (int) (l * l / 64f - 4 * l + 256)); // quadratic - if (l >= 128) { - modColorMap5MC.put(key, new Color(baseColor.getRed(), baseColor.getGreen(), baseColor.getBlue(), alpha)); - } else { - modColorMap5MC.put(key, new Color(noModColor5MC.getRed(), noModColor5MC.getGreen(), noModColor5MC.getBlue(), alpha)); - } - } - return modColorMap5MC.get(key); + public static Color getModColor(String modification, int l, AlignmentTrack.ColorOption colorOption) { - } else { - if (l > 250) { - return baseColor; - } - double threshold = 256 * PreferencesManager.getPreferences().getAsFloat("SAM.BASEMOD_THRESHOLD"); - if (l < threshold) { - l = 0; - } - if (!modColorMap.containsKey(key)) { - modColorMap.put(key, new Color(baseColor.getRed(), baseColor.getGreen(), baseColor.getBlue(), l)); - } - return modColorMap.get(key); - } - } - - public static Color getNoModColor(byte likelihood) { + if(colors.isEmpty()) updateColors(); // Note the pallete will always return a color, either an initially seeded one if supplied or a random color. - Color baseColor = noModColor5MC; + Color baseColor = getBaseColor(modification); - int l = Byte.toUnsignedInt(likelihood); - if (l > 255) { + if (l > 210) { return baseColor; } - String key = "NOMOD--" + l; - - if (!modColorMap5MC.containsKey(key)) { - int alpha = Math.min(255, (int) (l * l / 64f - 4 * l + 256)); // quadratic - modColorMap5MC.put(key, new Color(baseColor.getRed(), baseColor.getGreen(), baseColor.getBlue(), alpha)); + String key = modification + "--" + l; + if (!modColorMap.containsKey(key)) { + int alpha = Math.max(20, Math.min(255, (int) (l * l / 64f - 4 * l + 256))); + modColorMap.put(key, new Color(baseColor.getRed(), baseColor.getGreen(), baseColor.getBlue(), alpha)); } - - return modColorMap5MC.get(key); + return modColorMap.get(key); } - private static Color getBaseColor(String modification, AlignmentTrack.ColorOption colorOption) { - if ((colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_5MC || - colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_C) && colors5MC.containsKey(modification)) { - return colors5MC.get(modification); - } else if (colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_6MA) { - return color6ma; - } else if (colors.containsKey(modification)) { + + private static Color getBaseColor(String modification) { + if (colors.containsKey(modification)) { return colors.get(modification); } else { return genericColor; diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java index d70c6d1964..a5baf56b36 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java @@ -3,6 +3,7 @@ import org.broad.igv.sam.Alignment; import org.broad.igv.sam.AlignmentBlock; import org.broad.igv.sam.AlignmentTrack; +import org.broad.igv.util.collections.ByteArrayList; import java.util.*; @@ -17,21 +18,15 @@ public class BaseModificationCounts { */ LinkedHashSet allModifications; - /** - * Map for counts for each modification (e.g. m, h, etc). Key is base+modification identifier, value is map of position -> count - */ - Map> counts; - /** - * Map for capturing modification likelihood "pileup", key is modification identifier, - * value is map of position -> sum of likelihoods for modifications at that position - */ - Map> likelihoodSums; + Map> likelihoods; + + transient float lastThreshold; + public BaseModificationCounts() { allModifications = new LinkedHashSet<>(); - counts = new HashMap<>(); - likelihoodSums = new HashMap<>(); + likelihoods = new HashMap<>(); } /** @@ -53,96 +48,97 @@ public void incrementCounts(Alignment alignment) { // Loop through read sequence index ("i") for (int i = block.getBases().startOffset; i < block.getBases().startOffset + block.getBases().length; i++) { - for (BaseModificationSet bmset : baseModificationSets) { - - //String modification = bmset.getModification(); - BaseModificationKey key = BaseModificationKey.getKey(bmset.getBase(), bmset.getStrand(), bmset.getModification()); - Map likelihoods = bmset.getLikelihoods(); - - if (bmset.containsPosition(i)) { - - int lh = Byte.toUnsignedInt(likelihoods.get(i)); - - Map modCounts = counts.get(key); - if (modCounts == null) { - modCounts = new HashMap<>(); - counts.put(key, modCounts); - } - - Map modLikelihoods = likelihoodSums.get(key); - if (modLikelihoods == null) { - modLikelihoods = new HashMap<>(); - likelihoodSums.put(key, modLikelihoods); - } - - int blockIdx = i - block.getBases().startOffset; - int position = block.getStart() + blockIdx; // genomic position - - int c = modCounts.containsKey(position) ? modCounts.get(position) + 1 : 1; - - - int l = modLikelihoods.containsKey(position) ? modLikelihoods.get(position) + lh : lh; - modCounts.put(position, c); - modLikelihoods.put(position, l); - + int blockIdx = i - block.getBases().startOffset; + int position = block.getStart() + blockIdx; + + // Loop through base modification sets + char canonicalBase = 0; + int noModLH = 255; + for (BaseModificationSet bmSet : baseModificationSets) { + final Map bmSetLikelihoods = bmSet.getLikelihoods(); + if (bmSetLikelihoods != null && bmSet.containsPosition(i)) { // TODO or flag == '.' and readbase = bmSet.canonicalbase + byte byteLikelihood = bmSetLikelihoods.get(i); + BaseModificationKey modKey = BaseModificationKey.getKey(bmSet.getBase(), bmSet.getStrand(), bmSet.getModification()); + allModifications.add(modKey); + pushLikelihood(position, byteLikelihood, modKey); + + canonicalBase = bmSet.getCanonicalBase(); // Assumed same for all modifications at this position, modificatons on both bases at a position not supported + noModLH -= Byte.toUnsignedInt(byteLikelihood); } - allModifications.add(key); } + + if (canonicalBase != 0) { + BaseModificationKey noModKey = BaseModificationKey.getKey(canonicalBase, '+', "NONE_" + canonicalBase); + allModifications.add(noModKey); + pushLikelihood(position, (byte) noModLH, noModKey); + } + } } } } - public int getCount(int position, BaseModificationKey key) { - Map modCounts = counts.get(key); - if (modCounts != null && modCounts.containsKey(position)) { - return modCounts.get(position); - } else { - return 0; + private void pushLikelihood(int position, byte byteLikelihood, BaseModificationKey modKey) { + Map t = likelihoods.get(modKey); + if (t == null) { + t = new HashMap<>(); + likelihoods.put(modKey, t); + } + ByteArrayList byteArrayList = t.get(position); + if (byteArrayList == null) { + byteArrayList = new ByteArrayList(100); + t.put(position, byteArrayList); } + byteArrayList.add(byteLikelihood); } - public int getLikelhoodSum(int position, BaseModificationKey key) { - Map modLikelihoods = likelihoodSums.get(key); - if (modLikelihoods != null && modLikelihoods.containsKey(position)) { - return modLikelihoods.get(position); + public int getCount(int position, BaseModificationKey key, float threshold) { + lastThreshold = threshold; + float scaledThreshold = threshold * 255; + Map t = likelihoods.get(key); + ByteArrayList byteArrayList = t.get(position); + if (byteArrayList == null) { + return 0; } else { - return getCount(position, key) * 255; + int count = 0; + for (int i = 0; i < byteArrayList.size(); i++) { + int lh = Byte.toUnsignedInt(byteArrayList.get(i)); + if (lh > scaledThreshold) { + count++; + } + } + return count; } } + public Set getAllModificationKeys() { return allModifications; } public String getValueString(int position, AlignmentTrack.ColorOption colorOption) { StringBuffer buffer = new StringBuffer(); - for (Map.Entry> entry : counts.entrySet()) { + + // /** + // * Map for capturing modification likelihood "pileup", key is modification identifier, + // * value is map of position -> sum of likelihoods for modifications at that position + // */ + // Map> likelihoodSums; + + + for (Map.Entry> entry : likelihoods.entrySet()) { BaseModificationKey key = entry.getKey(); - Map modCounts = entry.getValue(); - if (modCounts.containsKey(position)) { - final Integer count = modCounts.get(position); - int lh = (int) (((100.0f / 255) * getLikelhoodSum(position, entry.getKey())) / count); - buffer.append("Modification: " + key.modification + " (" + key.base + key.strand + ", " + count + " @ " + lh + "%)
"); + Map t = entry.getValue(); + if (t.containsKey(position)) { + if(key.modification.startsWith("NONE_")) continue; + int count = this.getCount(position, key, lastThreshold); + if(count > 0) { + String modName = BaseModificationUtils.modificationName(key.modification); + buffer.append("Modification " + modName + " (" + key.base + key.strand + "): " + count + " with likelihood > " + (lastThreshold * 100) + "%
"); + } } } - return buffer.toString(); - } - /** - * For debugging - */ - public void dump() { - for (Map.Entry> entry : counts.entrySet()) { - - String modification = entry.getKey().toString(); - Map modCounts = entry.getValue(); - System.out.println("Modification: " + modification); - for (Map.Entry modKey : modCounts.entrySet()) { - System.out.println(modKey.getKey() + " " + modKey.getValue()); - } - } + return buffer.toString(); } - - } diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java index e0e137d0e5..e19eada3c4 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java @@ -1,16 +1,16 @@ package org.broad.igv.sam.mods; +import htsjdk.samtools.util.SequenceUtil; import org.broad.igv.sam.AlignmentCounts; import org.broad.igv.sam.AlignmentTrack.ColorOption; import org.broad.igv.track.RenderContext; import java.awt.*; import java.util.*; +import java.util.List; public class BaseModificationCoverageRenderer { - - public static void drawModifications(RenderContext context, int pX, int pBottom, @@ -19,179 +19,57 @@ public static void drawModifications(RenderContext context, int pos, AlignmentCounts alignmentCounts, ColorOption colorOption, - String basemodFilter) { - - switch (colorOption) { - case BASE_MODIFICATION_5MC: - draw5MC(context, pX, pBottom, dX, barHeight, pos, alignmentCounts, false); - break; - case BASE_MODIFICATION_C: - draw5MC(context, pX, pBottom, dX, barHeight, pos, alignmentCounts, true); - break; - case BASE_MODIFICATION_6MA: - draw(context, pX, pBottom, dX, barHeight, pos, alignmentCounts, "a"); - break; - default: - draw(context, pX, pBottom, dX, barHeight, pos, alignmentCounts, basemodFilter); - } - } - + BaseModficationFilter filter, + float threshold, + Set simplexModifications) { - private static void draw(RenderContext context, - int pX, - int pBottom, - int dX, - int barHeight, - int pos, - AlignmentCounts alignmentCounts, - String filter) { BaseModificationCounts modificationCounts = alignmentCounts.getModifiedBaseCounts(); if (modificationCounts != null) { - final BaseModificationKey mCKey = BaseModificationKey.getKey('C', '+', "m"); - final BaseModificationKey mGKey = BaseModificationKey.getKey('G', '-', "m"); + float modThreshold = threshold; + float nomodThreshold = threshold < 0.5 ? 1 - threshold : threshold; - Graphics2D graphics = context.getGraphics(); + Set allModificationKeys = modificationCounts.getAllModificationKeys(); + List sortedKeys = new ArrayList<>(allModificationKeys); + Collections.sort(sortedKeys); - Set allModificationKeys = modificationCounts.getAllModificationKeys(); - boolean cpgMode = allModificationKeys.contains(mCKey) && !allModificationKeys.contains(mGKey); - - // Merge complementary sets (same modification, opposite strands) - Map likelihoodSums = new LinkedHashMap<>(); - for (BaseModificationKey key : allModificationKeys) { - String modification = key.getModification(); - if(filter != null && !filter.equals(modification)) continue; - float currentCount = likelihoodSums.containsKey(modification) ? likelihoodSums.get(modification) : 0; - likelihoodSums.put(modification, currentCount + modificationCounts.getLikelhoodSum(pos, key)); - } - - // Color bar by likelihood weighted count + // Color bar modification counts (# of modifications > threshold) int total = alignmentCounts.getTotalCount(pos); - for (String modification : likelihoodSums.keySet()) { - - if (likelihoodSums.get(modification) > 0) { - - float modFraction; - if (cpgMode & "m".equals(modification)) { - // Special mode for out-of-spec 5mC CpG convention. - // Calls are made for the CG dinucleotide and only recorded for the canonical "C". We adjust the height - // of the bar to account for the missing G- calls. - final byte base = (byte) 'C'; - final byte compl = (byte) 'G'; - final int posCount = alignmentCounts.getPosCount(pos, base); - final int negCount = alignmentCounts.getNegCount(pos, compl); - - // Is this a "C" or "G" reference site? We want to determine this from the counts data - // directly, this should work for all but pathological edge cases - final int cCounts = alignmentCounts.getCount(pos, base); - final int gCounts = alignmentCounts.getCount(pos, compl); - final boolean cSite = cCounts > gCounts; - final int referenceCounts = cSite ? cCounts : gCounts; - final int strandCount = cSite ? posCount : negCount; - modFraction = (((float) referenceCounts) / total) * (likelihoodSums.get(modification) / (strandCount * 255f)); - - } else { - modFraction = likelihoodSums.get(modification) / (total * 255f); - - } - int modHeight = Math.round(modFraction * barHeight); - - int baseY = pBottom - modHeight; - Color modColor = BaseModificationColors.getModColor(modification, (byte) 255, ColorOption.BASE_MODIFICATION); - graphics.setColor(modColor); - graphics.fillRect(pX, baseY, dX, modHeight); - pBottom = baseY; - } - } - } - } + for (BaseModificationKey key : sortedKeys) { - private static void draw5MC(RenderContext context, - int pX, - int pBottom, - int dX, - int barHeight, - int pos, - AlignmentCounts alignmentCounts, - boolean allMods) { + if (filter != null && !filter.pass(key.modification, key.getCanonicalBase())) continue; + if(key.modification.startsWith("NONE_") && colorOption != ColorOption.BASE_MODIFICATION_2COLOR) continue; - BaseModificationCounts modificationCounts = alignmentCounts.getModifiedBaseCounts(); + byte base = (byte) key.getBase(); + final byte compl = SequenceUtil.complement(base); + int modifiable = alignmentCounts.getCount(pos, base) + alignmentCounts.getCount(pos, compl); + int detectable = simplexModifications.contains(key.modification) ? + alignmentCounts.getPosCount(pos, base) + alignmentCounts.getNegCount(pos, compl) : + modifiable; - if (modificationCounts != null) { + if (detectable == 0) continue; //No informative reads - final byte base = (byte) 'C'; - final byte compl = (byte) 'G'; - - Map likelihoodSums = new HashMap<>(); - Map modCounts = new HashMap<>(); - for (BaseModificationKey key : modificationCounts.getAllModificationKeys()) { - - // This coloring mode is exclusively for "C" modifications - if (key.getCanonicalBase() != 'C') continue; - if (key.getModification().equals("m") || allMods) { - String mod = key.getModification(); - final int count = modificationCounts.getCount(pos, key); - if (count > 0) { - modCounts.put(mod, count); - final int likelhoodSum = modificationCounts.getLikelhoodSum(pos, key); - likelihoodSums.put(mod, likelhoodSum); - } - } - } + float t = key.modification.startsWith("NONE_") ? nomodThreshold : threshold; + int count = modificationCounts.getCount(pos, key, t); + float modFraction = (((float) modifiable) / total) * (((float) count) / detectable); + int modHeight = Math.round(modFraction * barHeight); - if (likelihoodSums.size() > 0) { - - // Special mode for out-of-spec 5mC CpG convention. - // Calls are made for the CG dinucleotide and only recorded on 1 strand. We adjust the height - // of the bar to account for the missing G- calls. This is an approximation and assumes the - // distribution of calls is ~ equal on both strands. - - // Is this a "C" or "G" reference site? We want to determine this from the counts data directly, - // this should work for all but pathological edge cases - final int cCounts = alignmentCounts.getCount(pos, base); - final int gCounts = alignmentCounts.getCount(pos, compl); - final boolean cSite = cCounts > gCounts; - final int referenceCounts = cSite ? cCounts : gCounts; - - // Compute "snp factor", ratio of count of base calls that could be modified to - // total count. This is normally close to 1, but can be less due non CG bases at this location (e.g. snps) - double snpFactor = ((double) referenceCounts) / alignmentCounts.getTotalCount(pos); - - double calledBarHeight = snpFactor * barHeight; - double t = referenceCounts * 255; // If all bases are called this is the total sum of all likelihoods, including "no mod" likelihood - - // Likelihood of no modification. It is assumed that the likelihood of no modification == (1 - sum(likelihood)) - //int c = Collections.max(modCounts.values()); - double noModProb = t; - for (String m : modCounts.keySet()) { - if (likelihoodSums.containsKey(m)) { - noModProb -= likelihoodSums.get(m); - } - } - - // Draw "no mod" bar - int noModHeight = (int) Math.round((noModProb / t) * calledBarHeight); - int baseY = pBottom - noModHeight; + int baseY = pBottom - modHeight; + Color modColor = BaseModificationColors.getModColor(key.modification, 255, colorOption); Graphics2D graphics = context.getGraphics(); - graphics.setColor(BaseModificationColors.noModColor5MC); - graphics.fillRect(pX, baseY, dX, noModHeight); - - // Loop through modifications drawing bar for each - String[] orderedMods = likelihoodSums.keySet().toArray(new String[0]); - Arrays.sort(orderedMods, (o1, o2) -> -1 * o1.compareTo(o2)); - for (String m : orderedMods) { - Color mColor = BaseModificationColors.getModColor(m, (byte) 255, ColorOption.BASE_MODIFICATION_5MC); - int mModHeight = (int) Math.round(((likelihoodSums.get(m)) / t) * calledBarHeight); - if (mModHeight > 0) { - baseY -= mModHeight; - graphics.setColor(mColor); - graphics.fillRect(pX, baseY, dX, mModHeight); - } - } + graphics.setColor(modColor); + graphics.fillRect(pX, baseY, dX, modHeight); + pBottom = baseY; + } } } + + static Map modRankOrder; + + + } diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationKey.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationKey.java index a83a3c0480..5a6cbbf3af 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationKey.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationKey.java @@ -7,7 +7,7 @@ import java.util.Map; import java.util.Objects; -public class BaseModificationKey { +public class BaseModificationKey implements Comparable { char base; char strand; String modification; @@ -24,7 +24,10 @@ public static BaseModificationKey getKey(char base, char strand, String modifica keyCache.put(name, key); return key; } + } + public static BaseModificationKey getNomodKey(char base) { + return getKey(base, '+', "NONE"); } private BaseModificationKey(char base, char strand, String modification) { @@ -66,4 +69,39 @@ public int hashCode() { public String toString() { return "" + base + strand + modification; } + + + static Map modificationRankOrder ; + + @Override + public int compareTo(Object o) { + BaseModificationKey otherKey = (BaseModificationKey) o; + String mod1 = this.modification; + String mod2 = otherKey.modification; + + if(mod1.equals(mod2)) { + return (byte) this.strand - (byte) otherKey.strand; + } + + if(modificationRankOrder == null) { + modificationRankOrder = new HashMap<>(); + String [] tmp = {"NONE_C", "NONE_T", "NONE_G", "NONE_A", "m", "h", "f", "c", "C", "g", "e", "b", "T", "U", "a", "A", "o", "G", "n", "N"}; + for(int idx = 0; idx < tmp.length; idx++) { + modificationRankOrder.put(tmp[idx], idx); + } + } + if (modificationRankOrder.containsKey(mod1) & modificationRankOrder.containsKey(mod2)) { + return modificationRankOrder.get(mod1) - modificationRankOrder.get(mod2); + } else if (modificationRankOrder.containsKey(mod1)) { + return 1; + } else if (modificationRankOrder.containsKey(mod2)) { + return -1; + } else { + return mod1.compareTo(mod2); + } + } + + + + } diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java index 8f07af07c7..dc1cb46610 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java @@ -16,40 +16,9 @@ public static void drawModifications( Rectangle rowRect, Graphics g, AlignmentTrack.ColorOption colorOption, - String basemodFilter) { + BaseModficationFilter filter, + float threshold) { - switch (colorOption) { - case BASE_MODIFICATION_5MC: - draw5mC(alignment, bpStart, locScale, rowRect, g, false); - break; - case BASE_MODIFICATION_C: - draw5mC(alignment, bpStart, locScale, rowRect, g, true); - break; - case BASE_MODIFICATION_6MA: - draw(alignment, bpStart, locScale, rowRect, g, "a"); - break; - default: - draw(alignment, bpStart, locScale, rowRect, g, basemodFilter); - } - - } - - /** - * Helper function for AlignmentRenderer. Draw base modifications over alignment. - * - * @param alignment - * @param bpStart - * @param locScale - * @param rowRect - * @param g - */ - private static void draw( - Alignment alignment, - double bpStart, - double locScale, - Rectangle rowRect, - Graphics g, - String filter) { List baseModificationSets = alignment.getBaseModificationSets(); @@ -72,123 +41,51 @@ private static void draw( continue; } - // Search all sets for modifications of this base. For now keeps mod with > probability - // TODO -- merge mods in some way - byte lh = 0; + // Search all sets for modifications of this base. Pick modification with largest likelhiood + int maxLh = 0; + int noModLh = 255; String modification = null; + char canonicalBase = 0; + boolean modificationFound = false; for (BaseModificationSet bmSet : baseModificationSets) { - if (filter == null || filter.equals(bmSet.getModification())) { - if (bmSet.containsPosition(i)) { - if (modification == null || Byte.toUnsignedInt(bmSet.getLikelihoods().get(i)) > Byte.toUnsignedInt(lh)) { - modification = bmSet.getModification(); - lh = bmSet.getLikelihoods().get(i); - } + if (bmSet.containsPosition(i)) { + int lh = Byte.toUnsignedInt(bmSet.getLikelihoods().get(i)); + // TODO ELSE if flag == '.' lh = 0 + noModLh -= lh; + if (modification == null || lh > maxLh) { + modification = bmSet.getModification(); + canonicalBase = bmSet.getCanonicalBase(); + maxLh = lh; + modificationFound = modificationFound || filter == null || filter.pass(modification, canonicalBase); } } } - if (modification != null) { - - Color c = BaseModificationColors.getModColor(modification, lh, AlignmentTrack.ColorOption.BASE_MODIFICATION); - g.setColor(c); - - // Expand narrow width to make more visible - if (dX < 3) { - dX = 3; - pX--; + if (modificationFound) { + float modThreshold = threshold * 255; + float nomodThreshold = (threshold < 0.5 ? 1 - threshold : threshold) * 255; + Color c = null; + if (modification != null && maxLh > modThreshold && + (filter == null || filter.pass(modification, canonicalBase))) /* || flag == . */ { + c = BaseModificationColors.getModColor(modification, maxLh, colorOption); + } else if (noModLh > nomodThreshold && colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR) { + c = BaseModificationColors.getModColor("NONE_" + canonicalBase, noModLh, colorOption); } - g.fillRect(pX, pY, dX, Math.max(1, dY - 2)); - } - } - } - } - } - - /** - * Helper function for AlignmentRenderer. Draw base modifications over alignment for "5mC" mode. - *

- * Notes: - * Designed primarily for visualization of 5mC modifications compatible with existing bisulfite seq viz - * - 5mC methylated bases colored red - * - Non modified bases colored blue - * - Other modificationc colored as defined in BaseModificationColors - *

- * If multiple modifications are specified for a base the modification with the highest probability is - * drawn. - * - * @param alignment - * @param bpStart - * @param locScale - * @param rowRect - * @param g - */ - private static void draw5mC( - Alignment alignment, - double bpStart, - double locScale, - Rectangle rowRect, - Graphics g, - boolean allMods) { - - List baseModificationSets = alignment.getBaseModificationSets(); - if (baseModificationSets != null) { - - for (AlignmentBlock block : alignment.getAlignmentBlocks()) { - // Compute bounds - int pY = (int) rowRect.getY(); - int dY = (int) rowRect.getHeight(); - int dX = (int) Math.max(1, (1.0 / locScale)); - - for (int i = block.getBases().startOffset; i < block.getBases().startOffset + block.getBases().length; i++) { - - int blockIdx = i - block.getBases().startOffset; - int pX = (int) ((block.getStart() + blockIdx - bpStart) / locScale); + if (c != null) { + g.setColor(c); - // Don't draw out of clipping rect - if (pX > rowRect.getMaxX()) { - break; - } else if (pX + dX < rowRect.getX()) { - continue; - } - - // Search all sets for modifications of this base, select modification with largest likelihood - int lh = -1; - String modification = null; - - // Compare likelihoods, including likelihood of no modification - int noModificationLikelihood = 255; - for (BaseModificationSet bmSet : baseModificationSets) { - - // This coloring mode is exclusively for "C" modifications, either 5mC or all C mods - if (bmSet.getCanonicalBase() != 'C') continue; - if (bmSet.getModification().equals("m") || allMods) { - if (bmSet.containsPosition(i)) { - int l = Byte.toUnsignedInt(bmSet.getLikelihoods().get(i)); - noModificationLikelihood -= l; - if (modification == null || l > lh) { - modification = bmSet.getModification(); - lh = l; - } + // Expand narrow width to make more visible + if (dX < 3) { + dX = 3; + pX--; } + g.fillRect(pX, pY, dX, Math.max(1, dY - 2)); } } - - if (modification != null) { - - Color c = noModificationLikelihood > lh ? - BaseModificationColors.getNoModColor((byte) noModificationLikelihood) : - BaseModificationColors.getModColor(modification, (byte) lh, AlignmentTrack.ColorOption.BASE_MODIFICATION_5MC); - g.setColor(c); - - // Expand narrow width to make more visible - if (dX < 3) { - dX = 3; - pX--; - } - g.fillRect(pX, pY, dX, Math.max(1, dY - 2)); - } } } } } } + + diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationSet.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationSet.java index 8be28f8028..6f5c37ee74 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationSet.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationSet.java @@ -9,15 +9,16 @@ public class BaseModificationSet { char base; char strand; - String modification; Map likelihoods; + char canonicalBase; public BaseModificationSet(char base, char strand, String modification, Map likelihoods) { this.base = base; this.modification = modification; this.strand = strand; this.likelihoods = likelihoods; + this.canonicalBase = strand == '+' ? base : (char) SequenceUtil.complement((byte) base); } public char getBase() { @@ -25,7 +26,7 @@ public char getBase() { } public char getCanonicalBase() { - return strand == '+' ? base : (char) SequenceUtil.complement((byte) base); + return canonicalBase; } public String getModification() { diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java index 5912a4190d..dcef7e6ec4 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java @@ -34,9 +34,10 @@ public class BaseModificationUtils { codeValues.put("A", "Unknown A"); codeValues.put("G", "Unknown G"); codeValues.put("N", "Unknown"); - } + public static Set cModifications = new HashSet<>(Arrays.asList("m", "h", "f", "c", "C")); + public static String valueString(String modification, byte likelihood) { int l = (int) (100.0 * Byte.toUnsignedInt(likelihood) / 255); diff --git a/src/main/java/org/broad/igv/util/collections/ByteArrayList.java b/src/main/java/org/broad/igv/util/collections/ByteArrayList.java new file mode 100644 index 0000000000..2a0fbb63eb --- /dev/null +++ b/src/main/java/org/broad/igv/util/collections/ByteArrayList.java @@ -0,0 +1,134 @@ +/* + * The MIT License (MIT) + * + * Copyright (c) 2007-2015 Broad Institute + * + * Permission is hereby granted, free of charge, to any person obtaining a copy + * of this software and associated documentation files (the "Software"), to deal + * in the Software without restriction, including without limitation the rights + * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell + * copies of the Software, and to permit persons to whom the Software is + * furnished to do so, subject to the following conditions: + * + * The above copyright notice and this permission notice shall be included in + * all copies or substantial portions of the Software. + * + * + * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR + * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, + * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE + * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER + * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, + * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN + * THE SOFTWARE. + */ + +package org.broad.igv.util.collections; + + +/** + * Author: jrobinso + * Date: Jul 22, 2010 + *

+ * ArrayList type collection for int types. Purpose is to avoid the need to create an object for each entry + * in the standard java collections. + */ +public class ByteArrayList { + + + private transient byte[] elements; + + private int size; + + + public ByteArrayList() { + this(100); + } + + public ByteArrayList(int initialCapacity) { + if (initialCapacity < 0) + throw new IllegalArgumentException("Illegal Capacity: " + initialCapacity); + this.elements = new byte[initialCapacity]; + } + + public ByteArrayList(byte[] elements) { + this.elements = elements; + size = elements.length; + } + + public void add(byte e) { + if (size + 1 >= elements.length) { + grow(); + } + elements[size++] = e; + } + + public void addAll(byte[] args) { + byte[] newElements = new byte[size + args.length]; + System.arraycopy(elements, 0, newElements, 0, size); + System.arraycopy(args, 0, newElements, size, args.length); + elements = newElements; + size += args.length; + } + + public void addAll(ByteArrayList aList) { + addAll(aList.toArray()); + } + + public byte get(int idx) { + return elements[idx]; + } + + public int size() { + return size; + } + + public boolean isEmpty() { + return size == 0; + } + + /** + * Empty all elements. This logically clears the collection but does not free up any space. + */ + public void clear() { + size = 0; + } + + private void grow() { + int oldCapacity = elements.length; + int newCapacity; + if (oldCapacity < 10000000) { + newCapacity = oldCapacity * 2; + } else { + newCapacity = (oldCapacity * 3) / 2 + 1; + } + byte[] tmp = new byte[newCapacity]; + System.arraycopy(elements, 0, tmp, 0, elements.length); + elements = tmp; + } + + + public byte[] toArray() { + trimToSize(); + return elements; + } + + + private void trimToSize() { + int oldCapacity = elements.length; + if (size < oldCapacity) { + byte[] tmp = new byte[size]; + System.arraycopy(elements, 0, tmp, 0, size); + elements = tmp; + } + } + + public void set(int idx, byte i) { + while(idx >= elements.length) { + grow(); + } + elements[idx] = i; + idx++; + if(idx > size) size = idx; // Tried Math.max here, it showed up in cpu profiles! + } +} diff --git a/src/main/java/org/broad/igv/util/collections/IntArrayList.java b/src/main/java/org/broad/igv/util/collections/IntArrayList.java index 0235c4d4a5..a63a1452b9 100644 --- a/src/main/java/org/broad/igv/util/collections/IntArrayList.java +++ b/src/main/java/org/broad/igv/util/collections/IntArrayList.java @@ -25,7 +25,6 @@ package org.broad.igv.util.collections; -import org.broad.igv.util.IntComparator; /** * Author: jrobinso diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index 75fbe7fb43..a4d9ebc023 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -145,7 +145,6 @@ SAM.FILTER_URL URL or path to read group filter file string null ##Coverage Track Options SAM.ALLELE_THRESHOLD Coverage allele-fraction threshold float 0.2 SAM.ALLELE_USE_QUALITY Quality weight allele fraction boolean TRUE -SAM.BASEMOD_THRESHOLD Base modification average probability threshold (0-1) float 0 ##Insert Size Options SAM.COMPUTE_ISIZES Compute insert size thresholds boolean TRUE @@ -199,9 +198,34 @@ SAM.SHOW_INSERTION_MARKERS Show insertion markers boolean TRUE SAM.LINK_READS Link alignments by tag boolean FALSE SAM.LINK_TAG Linking tag string READNAME + +#Base Mods + +BASEMOD.THRESHOLD Base modification likelihood threshold (0-1) float 0.5 + +## Modified base colors +BASEMOD.M_COLOR 5mC color 255,0,0 +BASEMOD.H_COLOR 5hmC color 255,0,255 +BASEMOD.F_COLOR 5fC color 246,200,95 +BASEMOD.C_COLOR 5caC color 157,216,102 +BASEMOD.G_COLOR 5hmU color 255,160,86 +BASEMOD.E_COLOR 5fU color 141,221,208 +BASEMOD.B_COLOR 5caU color 0,100,47 +BASEMOD.A_COLOR 6mA color 51,0,111 +BASEMOD.O_COLOR 8oxoG color 111,78,129 +BASEMOD.OTHER_COLOR Other color 132,178,158 + +## Unomdified base colors (2-color option) +BASEMOD.NONE_A_COLOR A color 0,0,255 +BASEMOD.NONE_C_COLOR C color 0,0,255 +BASEMOD.NONE_T_COLOR T color 0,0,255 +BASEMOD.NONE_G_COLOR G color 0,0,255 +BASEMOD.NONE_N_COLOR G color 0,0,255 + ##SMRT Kinetics SMRT_KINETICS.SHOW_OPTIONS Show visibility options for SMRT kinetics data boolean FALSE + #Proxy PROXY.DISABLE_CHECK Disable check for system proxy boolean FALSE PROXY.USE Use proxy boolean FALSE diff --git a/src/test/java/org/broad/igv/sam/mods/BaseModficationFilterTest.java b/src/test/java/org/broad/igv/sam/mods/BaseModficationFilterTest.java new file mode 100644 index 0000000000..aa59f13558 --- /dev/null +++ b/src/test/java/org/broad/igv/sam/mods/BaseModficationFilterTest.java @@ -0,0 +1,32 @@ +package org.broad.igv.sam.mods; + +import org.junit.Test; + +import static org.junit.Assert.*; + +public class BaseModficationFilterTest { + + @Test + public void pass() { + BaseModficationFilter filter = new BaseModficationFilter(null, 'C'); + assertTrue(filter.pass(null, 'C')); + + String nomod = "NONE_C"; + assertTrue(filter.pass(nomod)); + } + + @Test + public void fromString() { + + String str = "hm,C"; + BaseModficationFilter filter = BaseModficationFilter.fromString(str); + + assertEquals("hm", filter.modification); + assertEquals('C', filter.base); + + assertTrue(filter.pass("m")); + assertTrue(filter.pass("h")); + assertTrue(filter.pass("NONE_C")); + assertFalse(filter.pass("a")); + } +} \ No newline at end of file diff --git a/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java b/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java index 54495ae2f9..0c216ad442 100644 --- a/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java +++ b/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java @@ -41,11 +41,40 @@ public void incrementCounts() throws IOException { BaseModificationKey key = BaseModificationKey.getKey('C', '+', "m"); for(int i=0; i bamiter = bamreader.query(chr, start, end, false); + int readCount = 0; + + BaseModificationCounts counts = new BaseModificationCounts(); + while (bamiter.hasNext()) { + Alignment alignment = bamiter.next(); + counts.incrementCounts(alignment); + readCount++; + } + assertTrue("No data retrieved: " + readCount, readCount > 0); - // counts.dump(); + BaseModificationKey cmKey = BaseModificationKey.getKey('C', '+', "m"); + int aboveThreshold = counts.getCount(119094723, cmKey, 0.5f); + assertEquals("Counts above threshold", 3, aboveThreshold); + BaseModificationKey noModKey = BaseModificationKey.getKey('C', '+', "NONE_C"); + int belowThreshold = counts.getCount( 119094723, noModKey, 0.5f); + assertEquals("Counts below threshold", 12, belowThreshold); } } \ No newline at end of file diff --git a/src/test/java/org/broad/igv/sam/mods/BaseModificationsTest.java b/src/test/java/org/broad/igv/sam/mods/BaseModificationsTest.java index e05ac4125b..84133d2cf7 100644 --- a/src/test/java/org/broad/igv/sam/mods/BaseModificationsTest.java +++ b/src/test/java/org/broad/igv/sam/mods/BaseModificationsTest.java @@ -70,6 +70,7 @@ public void testOrientTopRev() { // ....45..........6.......4...8.0..... //top-rev 16 * 0 0 * * 0 0 ATATGGCATATCCCCCGCCGATCCGCTAGAGATCCT * Mm:Z:C+m,1,3,0; Ml:B:C,128,153,179 + //sequence actually read TATACCGTATAGGGGGCGGCTAGGCGATCTCTAGGT <= read direction byte[] sequence = "ATATGGCATATCCCCCGCCGATCCGCTAGAGATCCT".getBytes(); String mm = "C+m,1,3,0"; byte[] ml = {(byte) 128, (byte) 153, (byte) 179}; From 6c8859b0a58e056883f5947b2527b99df4d7d79f Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Fri, 14 Jul 2023 09:04:56 -0700 Subject: [PATCH 074/116] Add test session for 10X MM bug (#1305) --- test/sessions/10x_sc_session.xml | 25 +++++++++++++++++++++++++ 1 file changed, 25 insertions(+) create mode 100644 test/sessions/10x_sc_session.xml diff --git a/test/sessions/10x_sc_session.xml b/test/sessions/10x_sc_session.xml new file mode 100644 index 0000000000..af3a6d7df1 --- /dev/null +++ b/test/sessions/10x_sc_session.xml @@ -0,0 +1,25 @@ + + + + + + + + + + + + + + + + + + + + + + + + + From 2e03ee4a13f41034225849fbeddf626fc4982c3e Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Tue, 1 Aug 2023 22:28:27 -0700 Subject: [PATCH 075/116] Fix broken merge --- src/main/java/org/broad/igv/sam/AlignmentDataManager.java | 8 +++++--- 1 file changed, 5 insertions(+), 3 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentDataManager.java b/src/main/java/org/broad/igv/sam/AlignmentDataManager.java index cb50c3ace5..222fe3a55f 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentDataManager.java +++ b/src/main/java/org/broad/igv/sam/AlignmentDataManager.java @@ -71,6 +71,7 @@ public class AlignmentDataManager implements IGVEventObserver { private SpliceJunctionHelper.LoadOptions loadOptions; private Set allBaseModificationKeys = new HashSet<>(); + private Set simplexBaseModfications = new HashSet<>(); private Range currentlyLoading; @@ -200,10 +201,8 @@ public Map getPEStats() { /** * Return all base modfications seen in loaded alignments */ - public Set getAllBaseModificationKeys() { return allBaseModificationKeys; - } public boolean isPairedEnd() { @@ -412,6 +411,10 @@ AlignmentInterval loadInterval(String chr, int start, int end, AlignmentTrack.Re private void updateBaseModfications(List alignments) { + for(Alignment a : alignments) { + List bmSets = a.getBaseModificationSets(); + if(bmSets != null) { + for(BaseModificationSet bms : bmSets) { allBaseModificationKeys.add(BaseModificationKey.getKey(bms.getBase(), bms.getStrand(), bms.getModification())); } } @@ -429,7 +432,6 @@ private void updateBaseModfications(List alignments) { simplexBaseModfications.add(entries.getKey()); simplexBaseModfications.add("NONE_" + entries.getValue().getCanonicalBase()); } - } public AlignmentTrack.ExperimentType inferType() { From b6cb0dd228a5cca11caabb07b7b46e422f2b2b18 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 3 Aug 2023 16:08:56 -0700 Subject: [PATCH 076/116] Coverage track popup text improvements for base mods --- src/main/java/org/broad/igv/sam/BaseAlignmentCounts.java | 1 - src/main/java/org/broad/igv/sam/CoverageTrack.java | 2 +- .../java/org/broad/igv/sam/mods/BaseModificationCounts.java | 4 +++- 3 files changed, 4 insertions(+), 3 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/BaseAlignmentCounts.java b/src/main/java/org/broad/igv/sam/BaseAlignmentCounts.java index 5465f0b76d..936ac7a653 100644 --- a/src/main/java/org/broad/igv/sam/BaseAlignmentCounts.java +++ b/src/main/java/org/broad/igv/sam/BaseAlignmentCounts.java @@ -206,7 +206,6 @@ public String getValueStringAt(int pos) { int delCount = getDelCount(pos); int insCount = getInsCount(pos); if (delCount > 0 || insCount > 0) { - buf.append("
---------------"); buf.append("
DEL: " + delCount); buf.append("
INS: " + insCount); } diff --git a/src/main/java/org/broad/igv/sam/CoverageTrack.java b/src/main/java/org/broad/igv/sam/CoverageTrack.java index c3c70043a3..7dc9a68fb3 100644 --- a/src/main/java/org/broad/igv/sam/CoverageTrack.java +++ b/src/main/java/org/broad/igv/sam/CoverageTrack.java @@ -375,7 +375,7 @@ public String getValueStringAt(String chr, double position, int mouseX, int mous buf.append(counts.getValueStringAt((int) position)); final AlignmentTrack.ColorOption colorOption = alignmentTrack.getRenderOptions().getColorOption(); if (colorOption.isBaseMod() && counts.getModifiedBaseCounts() != null) { - buf.append("


"); + buf.append("
"); buf.append(counts.getModifiedBaseCounts().getValueString((int) position, colorOption)); } } diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java index a5baf56b36..dfe14fe589 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java @@ -126,6 +126,8 @@ public String getValueString(int position, AlignmentTrack.ColorOption colorOptio // Map> likelihoodSums; + buffer.append("
---------
"); + buffer.append("Modifications with likelihood > " + (lastThreshold * 100) + "%"); for (Map.Entry> entry : likelihoods.entrySet()) { BaseModificationKey key = entry.getKey(); Map t = entry.getValue(); @@ -134,7 +136,7 @@ public String getValueString(int position, AlignmentTrack.ColorOption colorOptio int count = this.getCount(position, key, lastThreshold); if(count > 0) { String modName = BaseModificationUtils.modificationName(key.modification); - buffer.append("Modification " + modName + " (" + key.base + key.strand + "): " + count + " with likelihood > " + (lastThreshold * 100) + "%
"); + buffer.append("
  " + modName + " (" + key.base + key.strand + "): " + count ); } } } From 47b1b6266627624b8189130b2c7d74917bb6b5a6 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 3 Aug 2023 16:10:24 -0700 Subject: [PATCH 077/116] base mod menu rearrangement, default deprecated "5mC", "all C' to new options. --- .../org/broad/igv/sam/AlignmentTrack.java | 4 ++-- .../org/broad/igv/sam/AlignmentTrackMenu.java | 21 ++++++++++--------- .../BaseModificationCoverageRenderer.java | 10 ++++----- .../sam/mods/BaseModificationRenderer.java | 2 +- 4 files changed, 19 insertions(+), 18 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrack.java b/src/main/java/org/broad/igv/sam/AlignmentTrack.java index 1bed4a314d..f9c83bb5e6 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrack.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrack.java @@ -1760,10 +1760,10 @@ public void unmarshalXML(Element element, Integer version) { basemodFilter = new BaseModficationFilter("a"); } else if("BASE_MODIFICATION_5MC".equals(attributeValue)) { colorOption = ColorOption.BASE_MODIFICATION_2COLOR; - basemodFilter = new BaseModficationFilter(null, 'C'); + // basemodFilter = new BaseModficationFilter(null, 'C'); } else if("BASE_MODIFICATION_C".equals(attributeValue)) { colorOption = ColorOption.BASE_MODIFICATION; - basemodFilter = new BaseModficationFilter(null, 'C'); + // basemodFilter = new BaseModficationFilter(null, 'C'); }else { colorOption = ColorOption.valueOf(attributeValue); } diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index 028cfb1ac2..caaf223097 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -645,38 +645,39 @@ void addColorByMenuItem() { colorMenu.addSeparator(); if (allModifications.size() > 1) { - bmMenuItem = getColorMenuItem("base modification 2-color (all)", AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR); - bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR && filter == null); + bmMenuItem = getColorMenuItem("base modification (all)", AlignmentTrack.ColorOption.BASE_MODIFICATION); + bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION && filter == null); colorMenu.add(bmMenuItem); group.add(bmMenuItem); } for (String m : allModifications) { String name = BaseModificationUtils.modificationName(m); - bmMenuItem = getColorMenuItem("base modification 2-color (" + name + ")", AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR, m); - bmMenuItem.setSelected(renderOptions.getColorOption() == - AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR && - (filter != null && filter.pass(m))); + bmMenuItem = getColorMenuItem("base modification (" + name + ")", AlignmentTrack.ColorOption.BASE_MODIFICATION, m); + bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION && (filter != null && filter.pass(m))); colorMenu.add(bmMenuItem); group.add(bmMenuItem); } colorMenu.addSeparator(); if (allModifications.size() > 1) { - bmMenuItem = getColorMenuItem("base modification (all)", AlignmentTrack.ColorOption.BASE_MODIFICATION); - bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION && filter == null); + bmMenuItem = getColorMenuItem("base modification 2-color (all)", AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR); + bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR && filter == null); colorMenu.add(bmMenuItem); group.add(bmMenuItem); } for (String m : allModifications) { String name = BaseModificationUtils.modificationName(m); - bmMenuItem = getColorMenuItem("base modification (" + name + ")", AlignmentTrack.ColorOption.BASE_MODIFICATION, m); - bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION && (filter != null && filter.pass(m))); + bmMenuItem = getColorMenuItem("base modification 2-color (" + name + ")", AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR, m); + bmMenuItem.setSelected(renderOptions.getColorOption() == + AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR && + (filter != null && filter.pass(m))); colorMenu.add(bmMenuItem); group.add(bmMenuItem); } + } diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java index e19eada3c4..79358ab165 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java @@ -29,9 +29,9 @@ public static void drawModifications(RenderContext context, if (modificationCounts != null) { float modThreshold = threshold; - float nomodThreshold = threshold < 0.5 ? 1 - threshold : threshold; + float nomodThreshold = Math.max(0.5f, 1 - threshold); - Set allModificationKeys = modificationCounts.getAllModificationKeys(); + Set allModificationKeys = modificationCounts.getAllModificationKeys(); List sortedKeys = new ArrayList<>(allModificationKeys); Collections.sort(sortedKeys); @@ -41,7 +41,8 @@ public static void drawModifications(RenderContext context, for (BaseModificationKey key : sortedKeys) { if (filter != null && !filter.pass(key.modification, key.getCanonicalBase())) continue; - if(key.modification.startsWith("NONE_") && colorOption != ColorOption.BASE_MODIFICATION_2COLOR) continue; + if (key.modification.startsWith("NONE_") && colorOption != ColorOption.BASE_MODIFICATION_2COLOR) + continue; byte base = (byte) key.getBase(); final byte compl = SequenceUtil.complement(base); @@ -52,7 +53,7 @@ public static void drawModifications(RenderContext context, if (detectable == 0) continue; //No informative reads - float t = key.modification.startsWith("NONE_") ? nomodThreshold : threshold; + float t = key.modification.startsWith("NONE_") ? nomodThreshold : modThreshold; int count = modificationCounts.getCount(pos, key, t); float modFraction = (((float) modifiable) / total) * (((float) count) / detectable); int modHeight = Math.round(modFraction * barHeight); @@ -71,5 +72,4 @@ public static void drawModifications(RenderContext context, static Map modRankOrder; - } diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java index dc1cb46610..42f927b740 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java @@ -63,7 +63,7 @@ public static void drawModifications( if (modificationFound) { float modThreshold = threshold * 255; - float nomodThreshold = (threshold < 0.5 ? 1 - threshold : threshold) * 255; + float nomodThreshold = Math.max(0.5f, 1 - threshold) * 255; Color c = null; if (modification != null && maxLh > modThreshold && (filter == null || filter.pass(modification, canonicalBase))) /* || flag == . */ { From 2e86773e593cdcc39bf5beb20734c9f83d5b24b2 Mon Sep 17 00:00:00 2001 From: BOBAHDEP <5032319+BOBAHDEP@users.noreply.github.com> Date: Mon, 7 Aug 2023 19:00:58 +0200 Subject: [PATCH 078/116] Added empty line to 204 http response (#1368) Co-authored-by: Vladimir Ryzhikh --- src/main/java/org/broad/igv/batch/CommandListener.java | 2 ++ 1 file changed, 2 insertions(+) diff --git a/src/main/java/org/broad/igv/batch/CommandListener.java b/src/main/java/org/broad/igv/batch/CommandListener.java index ff0992fa46..5b3f6fffca 100755 --- a/src/main/java/org/broad/igv/batch/CommandListener.java +++ b/src/main/java/org/broad/igv/batch/CommandListener.java @@ -339,6 +339,8 @@ private void sendHTTPResponse(PrintWriter out, String result, String contentType out.print(result); out.print(CRLF); } + } else { + out.print(CRLF); } out.close(); } From 15d5dc791dc1904b120c0deec9d38f2effb24a2f Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Tue, 8 Aug 2023 17:59:04 -0700 Subject: [PATCH 079/116] test batch script for splice junctions --- test/batch/splice_junctions.txt | 5 +++++ 1 file changed, 5 insertions(+) create mode 100644 test/batch/splice_junctions.txt diff --git a/test/batch/splice_junctions.txt b/test/batch/splice_junctions.txt new file mode 100644 index 0000000000..8994f654ea --- /dev/null +++ b/test/batch/splice_junctions.txt @@ -0,0 +1,5 @@ +new +genome hg19 +load https://www.broadinstitute.org/igvdata/tutorials/SL2C5A3/liver.bodyMap.bam +goto slc25a3 +snapshot From bdc82464a02b477a2d167710b2cc9f8e8c6f63c0 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Fri, 11 Aug 2023 20:57:31 -0700 Subject: [PATCH 080/116] basenod refactorings Add optiong to group alignments by strand if basemod coloring is chosen -- defaults to true --- .gitignore | 2 + .../java/org/broad/igv/feature/Strand.java | 15 ++- .../java/org/broad/igv/prefs/Constants.java | 1 + .../org/broad/igv/sam/AlignmentTrackMenu.java | 30 +++-- .../igv/sam/mods/BaseModificationColors.java | 26 +++-- .../igv/sam/mods/BaseModificationCounts.java | 104 +++++++++++++----- .../BaseModificationCoverageRenderer.java | 13 ++- .../sam/mods/BaseModificationRenderer.java | 19 ++-- .../igv/sam/mods/BaseModificationUtils.java | 4 + .../java/org/broad/igv/track/TrackGroup.java | 7 +- .../org/broad/igv/prefs/preferences.tab | 3 +- .../sam/mods/BaseModificationCountsTest.java | 8 +- 12 files changed, 150 insertions(+), 82 deletions(-) diff --git a/.gitignore b/.gitignore index ece7bc2371..4c11921a08 100644 --- a/.gitignore +++ b/.gitignore @@ -20,3 +20,5 @@ out/ *.csi.gz *.sai /test/batch/snapshots/ +/src/main/java/org/broad/igv/sam/mods/BMScale.java +/src/main/java/org/broad/igv/sam/mods/BMScale.form diff --git a/src/main/java/org/broad/igv/feature/Strand.java b/src/main/java/org/broad/igv/feature/Strand.java index d07d9f2180..8c8666284e 100644 --- a/src/main/java/org/broad/igv/feature/Strand.java +++ b/src/main/java/org/broad/igv/feature/Strand.java @@ -25,9 +25,9 @@ /* -* To change this template, choose Tools | Templates -* and open the template in the editor. -*/ + * To change this template, choose Tools | Templates + * and open the template in the editor. + */ package org.broad.igv.feature; @@ -40,16 +40,21 @@ public enum Strand { private final String shortString; - private Strand(String shortString){ + private Strand(String shortString) { this.shortString = shortString; } + public static Strand fromString(String strandString) { return strandString.equals("+") || strandString.equalsIgnoreCase("POSITIVE") ? POSITIVE : (strandString.equals("-") || strandString.equalsIgnoreCase("NEGATIVE") ? NEGATIVE : NONE); } - public String toShortString(){ + public String toShortString() { return shortString; } + + public String toString() { + return this == POSITIVE ? "+" : this == NEGATIVE ? "-" : ""; + } } diff --git a/src/main/java/org/broad/igv/prefs/Constants.java b/src/main/java/org/broad/igv/prefs/Constants.java index a902105b8b..0594631106 100644 --- a/src/main/java/org/broad/igv/prefs/Constants.java +++ b/src/main/java/org/broad/igv/prefs/Constants.java @@ -200,6 +200,7 @@ private Constants() { public static final String BASEMOD_NONE_T_COLOR = "BASEMOD.NONE_T_COLOR"; public static final String BASEMOD_NONE_G_COLOR = "BASEMOD.NONE_G_COLOR"; public static final String BASEMOD_NONE_N_COLOR = "BASEMOD.NONE_N_COLOR"; + public static final String BASEMOD_GROUP_BY_STRAND = "BASEMOD.GROUP_BY_STRAND"; public static final String SMRT_KINETICS_SHOW_OPTIONS = "SMRT_KINETICS.SHOW_OPTIONS"; // Sequence track settings diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index caaf223097..385c178ffd 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -10,6 +10,7 @@ import org.broad.igv.lists.GeneList; import org.broad.igv.logging.LogManager; import org.broad.igv.logging.Logger; +import org.broad.igv.prefs.Constants; import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.sam.mods.BaseModficationFilter; import org.broad.igv.sam.mods.BaseModificationUtils; @@ -562,17 +563,26 @@ public void addFilterMenuItem() { } private JRadioButtonMenuItem getColorMenuItem(String label, final AlignmentTrack.ColorOption option) { - return getColorMenuItem(label, option, null); - + JRadioButtonMenuItem mi = new JRadioButtonMenuItem(label); + mi.setSelected(renderOptions.getColorOption() == option); + mi.addActionListener(aEvt -> { + alignmentTrack.setColorOption(option); + alignmentTrack.repaint(); + }); + return mi; } - private JRadioButtonMenuItem getColorMenuItem(String label, final AlignmentTrack.ColorOption option, String extra) { + private JRadioButtonMenuItem getBasemodColorMenuItem(String label, final AlignmentTrack.ColorOption option, boolean groupByStrand, String filter) { JRadioButtonMenuItem mi = new JRadioButtonMenuItem(label); mi.setSelected(renderOptions.getColorOption() == option); mi.addActionListener(aEvt -> { alignmentTrack.setColorOption(option); - renderOptions.setBasemodFilter(extra == null ? null : new BaseModficationFilter(extra)); - alignmentTrack.repaint(); + renderOptions.setBasemodFilter(filter == null ? null : new BaseModficationFilter(filter)); + if (groupByStrand) { + alignmentTrack.groupAlignments(AlignmentTrack.GroupOption.FIRST_OF_PAIR_STRAND, null, null); + } else { + alignmentTrack.repaint(); + } }); return mi; @@ -642,10 +652,10 @@ void addColorByMenuItem() { Set allModifications = dataManager.getAllBaseModificationKeys().stream().map(bmKey -> bmKey.getModification()).collect(Collectors.toSet()); if (allModifications.size() > 0) { BaseModficationFilter filter = renderOptions.getBasemodFilter(); - + boolean groupByStrand = alignmentTrack.getPreferences().getAsBoolean(BASEMOD_GROUP_BY_STRAND); colorMenu.addSeparator(); if (allModifications.size() > 1) { - bmMenuItem = getColorMenuItem("base modification (all)", AlignmentTrack.ColorOption.BASE_MODIFICATION); + bmMenuItem = getBasemodColorMenuItem("base modification (all)", AlignmentTrack.ColorOption.BASE_MODIFICATION, groupByStrand, null); bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION && filter == null); colorMenu.add(bmMenuItem); group.add(bmMenuItem); @@ -653,7 +663,7 @@ void addColorByMenuItem() { for (String m : allModifications) { String name = BaseModificationUtils.modificationName(m); - bmMenuItem = getColorMenuItem("base modification (" + name + ")", AlignmentTrack.ColorOption.BASE_MODIFICATION, m); + bmMenuItem = getBasemodColorMenuItem("base modification (" + name + ")", AlignmentTrack.ColorOption.BASE_MODIFICATION, groupByStrand, m); bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION && (filter != null && filter.pass(m))); colorMenu.add(bmMenuItem); group.add(bmMenuItem); @@ -661,7 +671,7 @@ void addColorByMenuItem() { colorMenu.addSeparator(); if (allModifications.size() > 1) { - bmMenuItem = getColorMenuItem("base modification 2-color (all)", AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR); + bmMenuItem = getBasemodColorMenuItem("base modification 2-color (all)", AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR, groupByStrand, null); bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR && filter == null); colorMenu.add(bmMenuItem); group.add(bmMenuItem); @@ -669,7 +679,7 @@ void addColorByMenuItem() { for (String m : allModifications) { String name = BaseModificationUtils.modificationName(m); - bmMenuItem = getColorMenuItem("base modification 2-color (" + name + ")", AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR, m); + bmMenuItem = getBasemodColorMenuItem("base modification 2-color (" + name + ")", AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR, groupByStrand, m); bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR && (filter != null && filter.pass(m))); diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationColors.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationColors.java index 8044199650..61877fe90e 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationColors.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationColors.java @@ -5,8 +5,12 @@ import org.broad.igv.prefs.Constants; import org.broad.igv.prefs.IGVPreferences; import org.broad.igv.prefs.PreferencesManager; +import org.broad.igv.sam.AlignmentRenderer; import org.broad.igv.sam.AlignmentTrack; +import org.broad.igv.ui.color.ColorUtilities; + import static org.broad.igv.prefs.Constants.*; + import java.awt.*; import java.util.HashMap; import java.util.Map; @@ -27,13 +31,11 @@ public class BaseModificationColors { static HashMap colors = new HashMap<>(); - // 5MC overrides -- avoid colors close to blue for "C" modificatoins - static HashMap colors5MC = new HashMap<>(); - + static Color neutralColor = new Color(195, 195, 195); static Color genericColor = new Color(132, 178, 158); - public static void updateColors () { - IGVPreferences preferences = PreferencesManager.getPreferences(); + public static void updateColors() { + IGVPreferences preferences = PreferencesManager.getPreferences(); colors.put("m", preferences.getAsColor(BASEMOD_M_COLOR)); colors.put("h", preferences.getAsColor(BASEMOD_H_COLOR)); colors.put("o", preferences.getAsColor(BASEMOD_O_COLOR)); @@ -50,6 +52,7 @@ public static void updateColors () { colors.put("NONE_T", preferences.getAsColor(BASEMOD_NONE_T_COLOR)); colors.put("NONE_G", preferences.getAsColor(BASEMOD_NONE_G_COLOR)); colors.put("NONE_N", preferences.getAsColor(BASEMOD_NONE_N_COLOR)); + modColorMap.clear(); } /** @@ -60,18 +63,17 @@ public static void updateColors () { public static Color getModColor(String modification, int l, AlignmentTrack.ColorOption colorOption) { - if(colors.isEmpty()) updateColors(); + if (colors.isEmpty()) updateColors(); // Note the pallete will always return a color, either an initially seeded one if supplied or a random color. Color baseColor = getBaseColor(modification); - if (l > 210) { - return baseColor; - } - - String key = modification + "--" + l; + String key = modification + l + colorOption; if (!modColorMap.containsKey(key)) { - int alpha = Math.max(20, Math.min(255, (int) (l * l / 64f - 4 * l + 256))); + int alpha = colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR ? + Math.max(20, Math.min(255, 20 + (int) (l * l / 50f - 4 * l + 200))) : + Math.max(20, (int) Math.min(255, 6.127e-3*l*l)); + modColorMap.put(key, new Color(baseColor.getRed(), baseColor.getGreen(), baseColor.getBlue(), alpha)); } return modColorMap.get(key); diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java index dfe14fe589..3bf83b0900 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java @@ -6,6 +6,7 @@ import org.broad.igv.util.collections.ByteArrayList; import java.util.*; +import java.util.stream.Collectors; /** * @author Jim Robinson @@ -18,20 +19,27 @@ public class BaseModificationCounts { */ LinkedHashSet allModifications; + /** + * Maxixum likelihood (i.e. maximum of all modifications present) for each position and base moodification key* + */ + Map> maxLikelihoods; - Map> likelihoods; + /** + * Likelihood of no modification (1 - sum(likelihoods)) for each position and base moodification key* + */ + Map> nomodLikelihoods; transient float lastThreshold; public BaseModificationCounts() { allModifications = new LinkedHashSet<>(); - likelihoods = new HashMap<>(); + maxLikelihoods = new HashMap<>(); + nomodLikelihoods = new HashMap<>(); } /** - * Increment modification counts for each position spanned by the supplied alignments. Currently both thresholded - * and total counts are tallied to support different coloring schemes. + * Increment modification counts for each position spanned by the supplied alignments. * * @param alignment */ @@ -45,40 +53,52 @@ public void incrementCounts(Alignment alignment) { for (AlignmentBlock block : alignment.getAlignmentBlocks()) { - // Loop through read sequence index ("i") - for (int i = block.getBases().startOffset; i < block.getBases().startOffset + block.getBases().length; i++) { + // Loop through read sequence for this block + for (int blockIdx = 0; blockIdx < block.getBases().length; blockIdx++) { - int blockIdx = i - block.getBases().startOffset; + int readIdx = block.getBases().startOffset + blockIdx; int position = block.getStart() + blockIdx; // Loop through base modification sets char canonicalBase = 0; + int maxLH = -1; + BaseModificationKey maxKey = null; int noModLH = 255; for (BaseModificationSet bmSet : baseModificationSets) { final Map bmSetLikelihoods = bmSet.getLikelihoods(); - if (bmSetLikelihoods != null && bmSet.containsPosition(i)) { // TODO or flag == '.' and readbase = bmSet.canonicalbase - byte byteLikelihood = bmSetLikelihoods.get(i); + if (bmSetLikelihoods != null && bmSet.containsPosition(readIdx)) { + byte byteLikelihood = bmSetLikelihoods.get(readIdx); BaseModificationKey modKey = BaseModificationKey.getKey(bmSet.getBase(), bmSet.getStrand(), bmSet.getModification()); allModifications.add(modKey); - pushLikelihood(position, byteLikelihood, modKey); - - canonicalBase = bmSet.getCanonicalBase(); // Assumed same for all modifications at this position, modificatons on both bases at a position not supported - noModLH -= Byte.toUnsignedInt(byteLikelihood); + int lh = Byte.toUnsignedInt(byteLikelihood); + noModLH -= lh; + if (lh > maxLH) { + canonicalBase = bmSet.getCanonicalBase(); // This has to be the same for all modifications at this position + maxLH = lh; + maxKey = modKey; + } } } + // Count the modification with highest likelihood, which might be the likelihood of no-modification if (canonicalBase != 0) { BaseModificationKey noModKey = BaseModificationKey.getKey(canonicalBase, '+', "NONE_" + canonicalBase); allModifications.add(noModKey); - pushLikelihood(position, (byte) noModLH, noModKey); - } + pushLikelihood(position, (byte) maxLH, maxKey, maxLikelihoods); + // 2-color counts, which include no-modification + if (noModLH > maxLH) { + pushLikelihood(position, (byte) noModLH, noModKey, nomodLikelihoods); + } else { + pushLikelihood(position, (byte) maxLH, maxKey, nomodLikelihoods); + } + } } } } } - private void pushLikelihood(int position, byte byteLikelihood, BaseModificationKey modKey) { + private void pushLikelihood(int position, byte byteLikelihood, BaseModificationKey modKey, Map> likelihoods) { Map t = likelihoods.get(modKey); if (t == null) { t = new HashMap<>(); @@ -92,10 +112,16 @@ private void pushLikelihood(int position, byte byteLikelihood, BaseModificationK byteArrayList.add(byteLikelihood); } - public int getCount(int position, BaseModificationKey key, float threshold) { + public int getCount(int position, BaseModificationKey key, float threshold, boolean includeNoMods) { + lastThreshold = threshold; - float scaledThreshold = threshold * 255; - Map t = likelihoods.get(key); + float scaledThreshold = threshold * 255; + + Map t = includeNoMods ? nomodLikelihoods.get(key) : maxLikelihoods.get(key); + if (t == null) { + return 0; + } + ByteArrayList byteArrayList = t.get(position); if (byteArrayList == null) { return 0; @@ -103,7 +129,7 @@ public int getCount(int position, BaseModificationKey key, float threshold) { int count = 0; for (int i = 0; i < byteArrayList.size(); i++) { int lh = Byte.toUnsignedInt(byteArrayList.get(i)); - if (lh > scaledThreshold) { + if (lh >= scaledThreshold) { count++; } } @@ -111,6 +137,25 @@ public int getCount(int position, BaseModificationKey key, float threshold) { } } + public int getLikelihoodSum(int position, BaseModificationKey key, float threshold, boolean includeNoMods) { + lastThreshold = threshold; + float scaledThreshold = threshold * 255; + Map t =includeNoMods ? nomodLikelihoods.get(key) : maxLikelihoods.get(key); + ByteArrayList byteArrayList = t.get(position); + if (byteArrayList == null) { + return 0; + } else { + int count = 0; + for (int i = 0; i < byteArrayList.size(); i++) { + int lh = Byte.toUnsignedInt(byteArrayList.get(i)); + if (lh >= scaledThreshold) { + count += lh; + } + } + return count; + } + } + public Set getAllModificationKeys() { return allModifications; @@ -128,19 +173,22 @@ public String getValueString(int position, AlignmentTrack.ColorOption colorOptio buffer.append("
---------
"); buffer.append("Modifications with likelihood > " + (lastThreshold * 100) + "%"); - for (Map.Entry> entry : likelihoods.entrySet()) { - BaseModificationKey key = entry.getKey(); - Map t = entry.getValue(); + + for (BaseModificationKey key : maxLikelihoods.keySet()) { + Map t = maxLikelihoods.get(key); + if (key.modification.startsWith("NONE_")) { + // continue; + } if (t.containsKey(position)) { - if(key.modification.startsWith("NONE_")) continue; - int count = this.getCount(position, key, lastThreshold); - if(count > 0) { + int count = this.getCount(position, key, lastThreshold, colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR); + if (count > 0) { + int likelihoodSum = getLikelihoodSum(position, key, lastThreshold, colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR); + int averageLikelihood = (int) ((((double) likelihoodSum) / count) * .3921568); String modName = BaseModificationUtils.modificationName(key.modification); - buffer.append("
  " + modName + " (" + key.base + key.strand + "): " + count ); + buffer.append("
  " + modName + " (" + key.base + key.strand + "): " + count + " @ average likelihood " + averageLikelihood + "%"); } } } - return buffer.toString(); } } diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java index 79358ab165..f6f5a1937c 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java @@ -28,9 +28,6 @@ public static void drawModifications(RenderContext context, if (modificationCounts != null) { - float modThreshold = threshold; - float nomodThreshold = Math.max(0.5f, 1 - threshold); - Set allModificationKeys = modificationCounts.getAllModificationKeys(); List sortedKeys = new ArrayList<>(allModificationKeys); Collections.sort(sortedKeys); @@ -53,13 +50,17 @@ public static void drawModifications(RenderContext context, if (detectable == 0) continue; //No informative reads - float t = key.modification.startsWith("NONE_") ? nomodThreshold : modThreshold; - int count = modificationCounts.getCount(pos, key, t); + int count = modificationCounts.getCount(pos, key, threshold, colorOption == ColorOption.BASE_MODIFICATION_2COLOR); + if (count == 0) continue; + float modFraction = (((float) modifiable) / total) * (((float) count) / detectable); int modHeight = Math.round(modFraction * barHeight); + int likelihoodSum = modificationCounts.getLikelihoodSum(pos, key, threshold, colorOption == ColorOption.BASE_MODIFICATION_2COLOR); + int averageLikelihood = (int) ((double) likelihoodSum) / count; + int baseY = pBottom - modHeight; - Color modColor = BaseModificationColors.getModColor(key.modification, 255, colorOption); + Color modColor = BaseModificationColors.getModColor(key.modification, averageLikelihood, colorOption); Graphics2D graphics = context.getGraphics(); graphics.setColor(modColor); graphics.fillRect(pX, baseY, dX, modHeight); diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java index 42f927b740..0501e06a70 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java @@ -46,30 +46,26 @@ public static void drawModifications( int noModLh = 255; String modification = null; char canonicalBase = 0; - boolean modificationFound = false; for (BaseModificationSet bmSet : baseModificationSets) { if (bmSet.containsPosition(i)) { int lh = Byte.toUnsignedInt(bmSet.getLikelihoods().get(i)); - // TODO ELSE if flag == '.' lh = 0 noModLh -= lh; - if (modification == null || lh > maxLh) { + if ((filter == null || filter.pass(bmSet.getModification(), canonicalBase)) && (modification == null || lh > maxLh)) { modification = bmSet.getModification(); canonicalBase = bmSet.getCanonicalBase(); maxLh = lh; - modificationFound = modificationFound || filter == null || filter.pass(modification, canonicalBase); } } } - if (modificationFound) { - float modThreshold = threshold * 255; - float nomodThreshold = Math.max(0.5f, 1 - threshold) * 255; + if (modification != null) { + Color c = null; - if (modification != null && maxLh > modThreshold && - (filter == null || filter.pass(modification, canonicalBase))) /* || flag == . */ { - c = BaseModificationColors.getModColor(modification, maxLh, colorOption); - } else if (noModLh > nomodThreshold && colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR) { + final float scaledThreshold = threshold * 255; + if (noModLh > maxLh && colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR && noModLh >= scaledThreshold) { c = BaseModificationColors.getModColor("NONE_" + canonicalBase, noModLh, colorOption); + } else if (maxLh >= scaledThreshold) { + c = BaseModificationColors.getModColor(modification, maxLh, colorOption); } if (c != null) { g.setColor(c); @@ -81,6 +77,7 @@ public static void drawModifications( } g.fillRect(pX, pY, dX, Math.max(1, dY - 2)); } + } } } diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java index dcef7e6ec4..8ae231aca3 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java @@ -34,6 +34,10 @@ public class BaseModificationUtils { codeValues.put("A", "Unknown A"); codeValues.put("G", "Unknown G"); codeValues.put("N", "Unknown"); + codeValues.put("NONE_C", "Unmodified"); + codeValues.put("NONE_T", "Unmodified"); + codeValues.put("NONE_G", "Unmodified"); + codeValues.put("NONE_A", "Unmodified"); } public static Set cModifications = new HashSet<>(Arrays.asList("m", "h", "f", "c", "C")); diff --git a/src/main/java/org/broad/igv/track/TrackGroup.java b/src/main/java/org/broad/igv/track/TrackGroup.java index b3b106bceb..693cd0e278 100644 --- a/src/main/java/org/broad/igv/track/TrackGroup.java +++ b/src/main/java/org/broad/igv/track/TrackGroup.java @@ -178,12 +178,7 @@ public void setDrawBorder(boolean drawBorder) { } public boolean isVisible() { - for (Track t : tracks) { - if ((t != null) && t.isVisible()) { - return true; - } - } - return false; + return tracks.stream().anyMatch(t -> t != null && t.isVisible()); } public int getHeight() { diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index a4d9ebc023..266896b970 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -201,7 +201,8 @@ SAM.LINK_TAG Linking tag string READNAME #Base Mods -BASEMOD.THRESHOLD Base modification likelihood threshold (0-1) float 0.5 +BASEMOD.THRESHOLD Base modification likelihood threshold (0-1) float 0 +BASEMOD.GROUP_BY_STRAND Group alignments by strand boolean TRUE ## Modified base colors BASEMOD.M_COLOR 5mC color 255,0,0 diff --git a/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java b/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java index 0c216ad442..5f405ff975 100644 --- a/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java +++ b/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java @@ -40,8 +40,9 @@ public void incrementCounts() throws IOException { BaseModificationKey key = BaseModificationKey.getKey('C', '+', "m"); + boolean includeNoMods = false; for(int i=0; i 0); BaseModificationKey cmKey = BaseModificationKey.getKey('C', '+', "m"); - int aboveThreshold = counts.getCount(119094723, cmKey, 0.5f); + int aboveThreshold = counts.getCount(119094723, cmKey, 0.5f, includeNoMods); assertEquals("Counts above threshold", 3, aboveThreshold); BaseModificationKey noModKey = BaseModificationKey.getKey('C', '+', "NONE_C"); - int belowThreshold = counts.getCount( 119094723, noModKey, 0.5f); + int belowThreshold = counts.getCount( 119094723, noModKey, 0.5f, includeNoMods); assertEquals("Counts below threshold", 12, belowThreshold); } } \ No newline at end of file From df6d12f9f2b2f61dd73ad6f9d39545b0fa22c81e Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Fri, 11 Aug 2023 21:27:16 -0700 Subject: [PATCH 081/116] Basemods -- add preference to indicate how skipped bases are treated, if not specified --- .../java/org/broad/igv/prefs/Constants.java | 4 +++- .../igv/sam/mods/BaseModificationSet.java | 4 ---- .../igv/sam/mods/BaseModificationUtils.java | 22 +++++++++---------- .../org/broad/igv/prefs/preferences.tab | 1 + 4 files changed, 15 insertions(+), 16 deletions(-) diff --git a/src/main/java/org/broad/igv/prefs/Constants.java b/src/main/java/org/broad/igv/prefs/Constants.java index 0594631106..b284207b14 100644 --- a/src/main/java/org/broad/igv/prefs/Constants.java +++ b/src/main/java/org/broad/igv/prefs/Constants.java @@ -201,6 +201,7 @@ private Constants() { public static final String BASEMOD_NONE_G_COLOR = "BASEMOD.NONE_G_COLOR"; public static final String BASEMOD_NONE_N_COLOR = "BASEMOD.NONE_N_COLOR"; public static final String BASEMOD_GROUP_BY_STRAND = "BASEMOD.GROUP_BY_STRAND"; + public static final String BASEMOD_SKIPPED_BASES = "BASEMOD.SKIPPED_BASES"; public static final String SMRT_KINETICS_SHOW_OPTIONS = "SMRT_KINETICS.SHOW_OPTIONS"; // Sequence track settings @@ -313,7 +314,8 @@ private Constants() { SAM_FILTER_SECONDARY_ALIGNMENTS, SAM_FILTER_SUPPLEMENTARY_ALIGNMENTS, SAM_JUNCTION_MIN_FLANKING_WIDTH, - SAM_JUNCTION_MIN_COVERAGE + SAM_JUNCTION_MIN_COVERAGE, + BASEMOD_SKIPPED_BASES ); /** diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationSet.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationSet.java index 6f5c37ee74..e53ffe9a2f 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationSet.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationSet.java @@ -56,10 +56,6 @@ public String valueString(int pos) { ((codeValues.containsKey(modification)) ? codeValues.get(modification) : "Uknown") + " (" + l + "%)"; } - public boolean is5mC() { - return modification.equals("m") && ((base == 'C' && strand == '+') || (base == 'G' && strand == '-')); - } - static Map codeValues; static { diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java index 8ae231aca3..3afec0da29 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java @@ -2,6 +2,8 @@ import org.broad.igv.logging.LogManager; import org.broad.igv.logging.Logger; +import org.broad.igv.prefs.Constants; +import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.sam.Alignment; import org.broad.igv.sam.AlignmentBlock; import org.broad.igv.sam.AlignmentTrack; @@ -40,15 +42,6 @@ public class BaseModificationUtils { codeValues.put("NONE_A", "Unmodified"); } - public static Set cModifications = new HashSet<>(Arrays.asList("m", "h", "f", "c", "C")); - - - public static String valueString(String modification, byte likelihood) { - int l = (int) (100.0 * Byte.toUnsignedInt(likelihood) / 255); - return "Base modification: " + modificationName(modification) + " (" + l + "%)"; - } - - public static String modificationName(String modification) { return ((codeValues.containsKey(modification)) ? codeValues.get(modification) : modification); } @@ -67,7 +60,6 @@ public static String modificationName(String modification) { */ public static List getBaseModificationSets(String mm, byte[] ml, byte[] sequence, boolean isNegativeStrand) { - byte[] origSequence = sequence; if (isNegativeStrand) { sequence = AlignmentUtils.reverseComplementCopy(sequence); } @@ -83,7 +75,15 @@ public static List getBaseModificationSets(String mm, byte[ String[] tokens = mmi.split(","); //Globals.commaPattern.split(mm); char base = tokens[0].charAt(0); char strand = tokens[0].charAt(1); - boolean skippedBasesCalled = tokens[0].endsWith("."); // False by default. + boolean skippedBasesCalled; + if(tokens[0].endsWith(".")) { + skippedBasesCalled = true; + } else if(tokens[0].endsWith("?")) { + skippedBasesCalled = false; + } else { + skippedBasesCalled = PreferencesManager.getPreferences().getAsBoolean(Constants.BASEMOD_SKIPPED_BASES); + } + if (tokens.length == 1) { // Legal but not handled yet, indicates modification is not present. Perhaps not relevant for visualization diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index 266896b970..294ed45a1f 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -203,6 +203,7 @@ SAM.LINK_TAG Linking tag string READNAME BASEMOD.THRESHOLD Base modification likelihood threshold (0-1) float 0 BASEMOD.GROUP_BY_STRAND Group alignments by strand boolean TRUE +BASEMOD.SKIPPED_BASES Assume skipped bases have low probability of modification. boolean FALSE ## Modified base colors BASEMOD.M_COLOR 5mC color 255,0,0 From 03335d354b9b964b5e09de1d95e6f2df651ed811 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Sat, 12 Aug 2023 11:00:58 -0700 Subject: [PATCH 082/116] fix unit test --- .../java/org/broad/igv/sam/mods/BaseModificationCountsTest.java | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java b/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java index 5f405ff975..6b67c2d74f 100644 --- a/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java +++ b/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java @@ -55,7 +55,7 @@ public void incrementCounts2() throws IOException { String chr = "chr11"; int start = 119094722; int end = 119094724; - boolean includeNoMods = false; + boolean includeNoMods = true; ResourceLocator locator = new ResourceLocator(bamfile); locator.setIndexPath(indexFile); From 5553b5161e7a1c305176ea22e48976129187f574 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 16 Aug 2023 23:03:14 -0700 Subject: [PATCH 083/116] Minor basemod fixes * Fix to "skipped bases" parsing at end of read * Menu change -- show "all" (unfiltered) base mod coloring options when only 1 mod present, as opposed to specific option for that mod --- .../org/broad/igv/sam/AlignmentTrack.java | 2 +- .../org/broad/igv/sam/AlignmentTrackMenu.java | 52 +++++++++---------- .../igv/sam/mods/BaseModificationColors.java | 4 +- .../igv/sam/mods/BaseModificationCounts.java | 2 +- .../BaseModificationCoverageRenderer.java | 6 ++- .../igv/sam/mods/BaseModificationKey.java | 3 -- .../igv/sam/mods/BaseModificationUtils.java | 16 +++--- .../igv/sam/mods/BaseModificationsTest.java | 34 ++++++++++-- 8 files changed, 72 insertions(+), 47 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrack.java b/src/main/java/org/broad/igv/sam/AlignmentTrack.java index f9c83bb5e6..b85ea3747e 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrack.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrack.java @@ -1841,7 +1841,7 @@ public void unmarshalXML(Element element, Integer version) { showInsertionMarkers = Boolean.parseBoolean(element.getAttribute("showInsertionMarkers")); } if (element.hasAttribute("basemodFilter")) { - basemodFilter = BaseModficationFilter.fromString(element.getAttribute("basemodfilter")); + basemodFilter = BaseModficationFilter.fromString(element.getAttribute("basemodFilter")); } if (element.hasAttribute("basemodThreshold")) { basemodFilter = BaseModficationFilter.fromString(element.getAttribute("basemodThreshold")); diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index 385c178ffd..cb3b45132e 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -654,37 +654,37 @@ void addColorByMenuItem() { BaseModficationFilter filter = renderOptions.getBasemodFilter(); boolean groupByStrand = alignmentTrack.getPreferences().getAsBoolean(BASEMOD_GROUP_BY_STRAND); colorMenu.addSeparator(); + String allModLabel = allModifications.size() > 1 ? "base modification (all)" : "base modification"; + bmMenuItem = getBasemodColorMenuItem(allModLabel, AlignmentTrack.ColorOption.BASE_MODIFICATION, groupByStrand, null); + bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION && filter == null); + colorMenu.add(bmMenuItem); + group.add(bmMenuItem); if (allModifications.size() > 1) { - bmMenuItem = getBasemodColorMenuItem("base modification (all)", AlignmentTrack.ColorOption.BASE_MODIFICATION, groupByStrand, null); - bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION && filter == null); - colorMenu.add(bmMenuItem); - group.add(bmMenuItem); - } - - for (String m : allModifications) { - String name = BaseModificationUtils.modificationName(m); - bmMenuItem = getBasemodColorMenuItem("base modification (" + name + ")", AlignmentTrack.ColorOption.BASE_MODIFICATION, groupByStrand, m); - bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION && (filter != null && filter.pass(m))); - colorMenu.add(bmMenuItem); - group.add(bmMenuItem); + for (String m : allModifications) { + String name = BaseModificationUtils.modificationName(m); + bmMenuItem = getBasemodColorMenuItem("base modification (" + name + ")", AlignmentTrack.ColorOption.BASE_MODIFICATION, groupByStrand, m); + bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION && (filter != null && filter.pass(m))); + colorMenu.add(bmMenuItem); + group.add(bmMenuItem); + } } colorMenu.addSeparator(); + allModLabel = allModifications.size() > 1 ? "base modification 2-color (all)" : "base modification 2-color"; + bmMenuItem = getBasemodColorMenuItem(allModLabel, AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR, groupByStrand, null); + bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR && filter == null); + colorMenu.add(bmMenuItem); + group.add(bmMenuItem); if (allModifications.size() > 1) { - bmMenuItem = getBasemodColorMenuItem("base modification 2-color (all)", AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR, groupByStrand, null); - bmMenuItem.setSelected(renderOptions.getColorOption() == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR && filter == null); - colorMenu.add(bmMenuItem); - group.add(bmMenuItem); - } - - for (String m : allModifications) { - String name = BaseModificationUtils.modificationName(m); - bmMenuItem = getBasemodColorMenuItem("base modification 2-color (" + name + ")", AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR, groupByStrand, m); - bmMenuItem.setSelected(renderOptions.getColorOption() == - AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR && - (filter != null && filter.pass(m))); - colorMenu.add(bmMenuItem); - group.add(bmMenuItem); + for (String m : allModifications) { + String name = BaseModificationUtils.modificationName(m); + bmMenuItem = getBasemodColorMenuItem("base modification 2-color (" + name + ")", AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR, groupByStrand, m); + bmMenuItem.setSelected(renderOptions.getColorOption() == + AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR && + (filter != null && filter.pass(m))); + colorMenu.add(bmMenuItem); + group.add(bmMenuItem); + } } diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationColors.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationColors.java index 61877fe90e..a96874caea 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationColors.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationColors.java @@ -31,8 +31,6 @@ public class BaseModificationColors { static HashMap colors = new HashMap<>(); - static Color neutralColor = new Color(195, 195, 195); - static Color genericColor = new Color(132, 178, 158); public static void updateColors() { IGVPreferences preferences = PreferencesManager.getPreferences(); @@ -85,7 +83,7 @@ private static Color getBaseColor(String modification) { if (colors.containsKey(modification)) { return colors.get(modification); } else { - return genericColor; + return colors.get("other"); } } diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java index 3bf83b0900..980ede1b37 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java @@ -25,7 +25,7 @@ public class BaseModificationCounts { Map> maxLikelihoods; /** - * Likelihood of no modification (1 - sum(likelihoods)) for each position and base moodification key* + * Maximum likelihood including no-modification (1 - sum(likelihoods)) for each position and base moodification key* */ Map> nomodLikelihoods; diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java index f6f5a1937c..0d475db9d6 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationCoverageRenderer.java @@ -37,7 +37,11 @@ public static void drawModifications(RenderContext context, for (BaseModificationKey key : sortedKeys) { - if (filter != null && !filter.pass(key.modification, key.getCanonicalBase())) continue; + try { + if (filter != null && !filter.pass(key.modification, key.getCanonicalBase())) continue; + } catch (Exception e) { + throw new RuntimeException(e); + } if (key.modification.startsWith("NONE_") && colorOption != ColorOption.BASE_MODIFICATION_2COLOR) continue; diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationKey.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationKey.java index 5a6cbbf3af..707bc8737e 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationKey.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationKey.java @@ -26,9 +26,6 @@ public static BaseModificationKey getKey(char base, char strand, String modifica } } - public static BaseModificationKey getNomodKey(char base) { - return getKey(base, '+', "NONE"); - } private BaseModificationKey(char base, char strand, String modification) { this.base = base; diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java index 3afec0da29..072f236fc7 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java @@ -76,9 +76,9 @@ public static List getBaseModificationSets(String mm, byte[ char base = tokens[0].charAt(0); char strand = tokens[0].charAt(1); boolean skippedBasesCalled; - if(tokens[0].endsWith(".")) { + if (tokens[0].endsWith(".")) { skippedBasesCalled = true; - } else if(tokens[0].endsWith("?")) { + } else if (tokens[0].endsWith("?")) { skippedBasesCalled = false; } else { skippedBasesCalled = PreferencesManager.getPreferences().getAsBoolean(Constants.BASEMOD_SKIPPED_BASES); @@ -117,8 +117,6 @@ public static List getBaseModificationSets(String mm, byte[ likelihoodMap.put(m, new HashMap<>()); } - - int nPositions = tokens.length - 1; int idx = 1; // position array index, positions start at index 1 int skip = Integer.parseInt(tokens[idx++]); @@ -129,7 +127,7 @@ public static List getBaseModificationSets(String mm, byte[ if (base == 'N' || sequence[p] == base) { int position = isNegativeStrand ? sequence.length - 1 - p : p; - if (matchCount == skip) { + if (matchCount == skip && idx < tokens.length) { for (String modification : modifications) { byte likelihood = ml == null ? (byte) 255 : ml[mlIdx++]; likelihoodMap.get(modification).put(position, likelihood); @@ -138,7 +136,11 @@ public static List getBaseModificationSets(String mm, byte[ skip = Integer.parseInt(tokens[idx++]); matchCount = 0; } else { - break; + if (!skippedBasesCalled) { + // If skipped bases are not called unmodified we are done. If they are we need + // to scan the entire sequence + break; + } } } else { if (skippedBasesCalled) { @@ -149,8 +151,8 @@ public static List getBaseModificationSets(String mm, byte[ likelihoodMap.get(modification).put(position, likelihood); } } - matchCount++; } + matchCount++; } p++; } diff --git a/src/test/java/org/broad/igv/sam/mods/BaseModificationsTest.java b/src/test/java/org/broad/igv/sam/mods/BaseModificationsTest.java index 84133d2cf7..24b2eb29e6 100644 --- a/src/test/java/org/broad/igv/sam/mods/BaseModificationsTest.java +++ b/src/test/java/org/broad/igv/sam/mods/BaseModificationsTest.java @@ -101,7 +101,7 @@ public void testOrientBotFwd() { byte[] sequence = "AGGATCTCTAGCGGATCGGCGGGGGATATGCCATAT".getBytes(); String mm = "G-m,0,0,4,3;"; byte[] ml = {(byte) 115, (byte) 141, (byte) 166, (byte) 192}; - for(byte b : ml) { + for (byte b : ml) { System.out.println(b); } boolean isNegativeStrand = false; @@ -207,7 +207,7 @@ public void testDouble() { String mm = "C+m,1,3,0;G-m,0,2,0,4;G+o,4"; byte[] ml = {(byte) 128, (byte) 153, (byte) 179, (byte) 115, (byte) 141, (byte) 166, (byte) 192, (byte) 102}; boolean isNegativeStrand = false; - + List modificationSets = BaseModificationUtils.getBaseModificationSets(mm, ml, sequence, isNegativeStrand); assertEquals(3, modificationSets.size()); @@ -428,15 +428,39 @@ public void test6mAAlignment() { List modificationSets = BaseModificationUtils.getBaseModificationSets(mm, null, seq.getBytes(), isNegativeStrand); assertEquals(2, modificationSets.size()); - for(BaseModificationSet bms : modificationSets) { + for (BaseModificationSet bms : modificationSets) { char base = bms.getBase(); Map likelihoods = bms.getLikelihoods(); List positions = new ArrayList<>(likelihoods.keySet()); - positions.sort((o1, o2) -> o1-o2); - for(Integer pos : positions) { + positions.sort((o1, o2) -> o1 - o2); + for (Integer pos : positions) { assertTrue(base == seq.charAt(pos)); } } } + /** + * Test skipped-bases (".") + */ + @Test + public void testSkippedBases() { + + String mm = "C+m.,182,60,61,3,60,30,3,43,26,13,20,25,6,6,3,15,51,1,47,15,0,118,38,3,90,167,28,2,53,38,40,109,0,16,3,62,32,0,11,0,0,1,9,11,9,11,0,9,21,1,5,12,0,120,5,25,39,60,1,0,2,3,2,4,7,14,62,23,1,89,12,20,2,134,2,2,31,33,30,1,265,93,23,77,22,25,0,6,74,47,0,33,18,2,108,106,0,10,1,46,111,25,91,2,1,11,15,13,1,19,5,0,92,58,0,11,5,103,23,5,1,29,7,25,34,92;"; + String seq = "CATCAGACAGTCTGGGTTGCTTGCACTGAAACCACATACAGTGTACTGGTGGTAGTCGGGGGAGACATTCTAAATCCTCAGAGTCACAAATAGCCCTGCAGCATTTTGTGTTTCTGCCAACCTGGGTAAAAGGGAGATGACTGGACTTACTGTTCACTCAAATGAGTTATTCCTGTAGTGATTGAGACTGTTCTTCAAAACGTTACAAATGTGGCCAGGCATGGTGGCTCATGCCTGTAATCCTAGCACTTTGGGAGGCTGAGGCAGGAGGATCACTTCAGCCCAGAAGTTCAGTACCAGCCTGGGCAACCTAGGGGGACCCCAATTCTACAAAAACAATTTGAAAATTAGCTGGGCATGCAGGCACGTGCCTGTGGCCCCAGCTACTCAGGAGGCTGAGGTGGGAGAATTGCTTGGGCCTCGGAGGTTGAGGCTGCAGTGAGCCGTGCTTGCATCACTGCACTTCAGTGTGGGGCGACAGAATGAGACCCTATTTCAAAAAGAAAAAAAATGTCTCTGGGCCACACACATTTGGGGTGGCTCAAGAGCAGTCCAGAGTTATCAGTCAGATCTTTAAGGTATGAGGTCTGCAGTACAGTGCACTGGGCCCTGGGGGAGGATTAGGTAGGCTATTCAGGGGTTGGGGAGGCACCCTGGGCCCTCTGGCTTGGCCTTCAGAGTTTCCAAGTGGTGGGGACTTGAGAGGAAGTTTGTCCTCTGTGGGTCAGGGAGCCCCGGGGCTGCTCCTCCCCTGGAACATTTATGCTTCTCAGGTTGAGGGCTGCTTCTCCTGGCCTGTGGCCTGGCGCTGTCTCCCTACACTGGGAAGCTGGCAAACCTCTCCCTTGGGGGTCTATGTATGCCCTACAGGAGCCACCCACCTTGACCTGGACTCTAGGGGCCATCTGGCATTCGGGATATTCCCTACAGGGACCCAGGCCGTGGAGCCGGGGACTGAATCTTTTTTTTTTTTTTTTTTTGAGATGGATCTTGCTCTGTCACCCAGGCTGGAGTGCAGTGGCATGATCTCGGCTCACTGCAACCTCTGTCTCCCTGGTTCAAGCGATTCTCCTGTCTCGGCCGCCCAAGTAGCTGGGATTATAGGCACGCGCCACCATGCCCAGCTAATTTTTGTATTTTTAGTAGATATGGGGTTTCATCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTTGAGTGGTCTACCTGCCTTGGACTCCCAAAGTGTTGGGATTACAGGCATGAGCCACCGCACCCGGCCAAAGGACTGAGTCTTTAGTGTGGTGCTGGTGTGGAAGTGTCAGACACATATGTGTGGCTGATTGACTTGGGGTGTGGGAGATCCTGCAATTATTCACACAGTCATGCTCCACCATGCAAAATCCCCATGCCTCTGTGGAGCACACTCCTGGAGGACTTCTGGGCCGGTAGCATCAAGCTCCCCACTCAGCCAGGGTCTTTCTATCTCAGCCTGGTCCTGAGACATCTGACGGATGGCGTTTTCTTTTACCATGTTGGCCAAGCTGGTCTCAAACTCCTGATCTTGTGATCCGCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGTCACTGCGCCTGGCCGGATGGAATTTTCTTTAATGAGAGAGAGAAGTTGATTGATAGAATCAAACAGTGATGTGTGGCAGATTCTGAGGCCTATATAGGTCCTGGGGGTGTTTTTCCTAAACCAGGAGTTCTAGCTCCTGAGAGATGGGTAGTTGAGTGGCCCAGATGGATCCTTGGATTGAAGCAGCCAAAATTACTTAGAGTGGGAGTGCCTAGGAAAAACTTCCTGCTGCTCCCTGCTCTCTGTAACAATTGTGTTTACACATATGTGTGTGTATACACGCACACATATGCACACATGGGCTGGCTGGGCCTGAGCCCAGAGAGCAACACGGAGGGTGGGCACCAGACCAGATGCTGGCGGGGGTGGGGGTGGAGGGCAGGCACGGGAGACGCAGGCAGAGTGAGTGTCTGCATATAGATGGCTGTTAGGGAGCCAAGGAAATGGCTTGGAAAATGTCCTTATTGGGTGTTTGCAGCTCCGGCTTTGTTGGGGTCGGCGCCTGGTGTGGGCAGATGGCCCTGTCTGCCTGTTGTTGCTGAGGGCTTCTTGCCTGCTGAGCCCGGTACGGCAGGCTCATACCGGGGAAGCAGCACCCTCTGCGTTTTGTGCCGTGTGTGGGCTTTGTGAATGTGAACCCGTTAGCCCTTACTCAGGTGGGGTGGTGGCCGCTGTCCCCATTTCACGGCAAAGAAGGGTGCAGAGGCCACACTAAGGTAGCGTCACTAGGGAGCAGCGGAGGCAGGGCTGTTTGGCAGCAGCAAAGCTGCACATGGAGCTGGCTGCCTCCGGGGTCACGTGGCCTGCATCTCCATGGGGGCTTGTGTGCAGTGGAGGGGGTTGCATGGCATGGCCCGGTGGCAAAAGAGGAGGCAGCCTCGGGAAGCCCAGGACCTTTCCCAGACCACAGTCCTGGGGGCATACTCAAGTCAGGGGGCGGGGACAGGAATGTGCTGGTGACTACATCACCTTGAGGCAGGTACCACCTCAATCCCGAACCGCCTGCCTTCTTCTCTGTCATTGCTGTCCTCCATTTCTTGTTATCAGCAAAGCTTCTGACAAAGCAAAAGTAGAGTCCCTTACTTCCCAGCTCCTTTCTTGGAGTCTGACCTTTCTGCCAGGTCTCCTTTGTCTGCTTCCCAAAACAAAACAGCGGACCAAAGCCAGGCACAGTGGCTCATGCCTATAATCCCAGCAGTTTGGAAGCCGAGGTGGGAGGATCGCTTGAGCTAAGGATTTCAAGACCAGCCTGGGCAACATGGCAAAACCCTGTCTCTATAAAAAACTAGCTGGGCATGGTGGCACATGCTTGAGGTCCCAGCTACCTAGGAGGCCGAGGTGGGAGGATCACTTGAGCCCTGGAGGTCAAGGCTGCAGTGAGCCGTGATGGCACCACTGTACTCCAGCCTGGGCAACAGAGTGAGACCCTATCTCAAAAATAGCCCAACAACAAACAACAGGCCAACGTCCCCCGCTGCAAGCTGGGCAGATGGCCAGGCTGGGCACACCACAGACTGTCTTCTGAGCAAACATGGGTGCCGCAGCACTGGGCCAACCTGAATAAAGAGCCTCATGGTTGAGGTGCGTATAGCAGGGGCTGGGGGCTTGGAGCAGACCTGGGAGCTGCATTCTCTGCCTTTTGAAACATCTGCTGGGCGGGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGTGGATCACCTGAGGTTGGGAGTTCGAGACCAGCCTGACCAACATGGAGAAACTCAGTCTCTACTAAAAATACAAAATTAGCTGGGCGTGGTAGCTCATGCCAGTAATTCCAGCTAGTTGGGAGGCTGAGACAGGAGAATCGCTTGATCCCGGGAAGCGGAGGTTGCCGTGAGCTGAGATTGTGCCATTGCATTCCAGCCTGGGCAACAAGAACGAAAACTCTGTCTCAAAAAACAAAAAAAAGAAAAAAGAAACATCTAAAGTCTGTCCCAGCCAAGCACTAACCAGGACCTTCTGTGAGCTGACGTCAGGACAGAGGCCCCCTCGGGGCCAGCTTTGATGTTTCCCAGGCCCAGTCGTTGGGGCACAGGGGGCTCTGAGTGGCAGTCTGGGAATGCAGCATCTCTCACCACAGCTATGGAGCAGTCCAGTCCCAGCCCCAGGAGTGGGTGCTGGAGGCTGAGCTGGGGGTGGCCGAGGCTGAGCTGGGGTCTCTCTGAGTCACGCTGTCTCTTCTCCCAGCTTGGGTTTCTGTGGCAGGTGGTCTGTGGTTCCTGCAAGCTCAGGGCCCCTTCCCCTCTATCCCACCCACCACCTGCTTATTGCCTTCAGACTTCTCTGCTGTGGCAGCTCCTTCCCTGGCCAGGGTCTGGGAGTGGTCCTGGCACTTGGCAAGTGGAGAGCCTTCTAATCTTCTTAGAAGTTGAGTCCTTGAGCAGGAGGGGTGATCATGAGAATATTAACAGTAGCTGCCATTTACTGAAGATGAACCATTTGCAAGGCACTTCATGTATGTTTCCAGGTTTACAACTCATGACAGCCTTGTGAGAGAGGCAGAGGAAGCTGAGACACAGTCTGTGTTACTTACCCAAGGTCAGACACACTGAGTGTAACAGGTCAGGATCTGAGCCCAGCCTGTCTCATTCCAGCCCCTTAGCTGCTCCTCTGTGTGGGTGAGACTCCTGGCTCCCCACCATGTCTGCAGAAGCTGAGCGAGGAAGTGGGGCTTGTCCACACTTCGAAGGCAGGTGATCTCTCTTTGGTCTTTGACAAGCCTCCTGGAGTTGTGGGTCAAAGATCCTAGGATTTTGGGCAGGGCGCCATGGCTCACATCTGTAATCCCAGCACTTTAGGAGGCCGAGGTGGGTGGATCACTTGAGGTCAGGAGCTCGAGACTAGCCTGGCCAACATGGTGAAACCCTGTCTCCACAAAAAATACAAAAATTAGCCAGGTGTGGTGGTGCGTAGCTGTAATCCCAGCTACTCGGGAGGCTAAGGCATGAGAATCGCTTAAATCCGAGAGGTGGAGGTTGCAGTGAGCCAAGATTGCACCACTGCACTCCAGCGTGGGTGGCAGAGTAAGTAAGACTCCGTCTCAAAAAAAAAGAAAGGTCCTAGGATTTTGGTGAGTCACATTTGCAGAGGCCCACTGGGGACTCAGTCTGATTCTGCCCATCTGTGTCATGAGAGGTGCTCCAGAAGTACCCCGTGAACCAGTTCTTGCCCCACGCTTCCTCAGAGGTCACTTTGGTTCAGGCAGTGCTTGCTGGGTGCCTTTGTGTGCCTGACATTGTCTGCAAGAGGTGTGTCCACACACTACCTGGCAGTCGGGAATGTGACTCCTCATTGTAGGATCAGATGCTTCCAGGGTTGTTTCTGAGTCCCTGGGCGGGGATGGGAAGAGCACAGAGTTGGTTTCTTTGGGTCATAGTCCTGGCTCTGCCACATATGAGCTATTTGATCGTGGGCAGATTCCTTCTTGTGCCTGGACCTGGGTTTACCCATCTGTACCACGGTGCTGAGCAGGAACAGAGCCCTATTCCTGTGGATCATTTGGAAATGTGTAGGGATGTGTTTGAATTGTCACAATGCCGGGAGGGCAGATTTGTTAACTTGCACAGCAGGTGGAATAGCTCACCCAGAGAACCATCAGTGCCACGCTGAGAAGTAATGGGCTGCTTGGAGCTGCTTCCAGCTCCATGACTGGGTTTTGTGTCAGGAGATGTGGGCTGGCACCTATGAGCATGTTGGGAGACGGACCAACACCAGGGGCAGCCCCTCATCTGGACCCAGCCTGGGGGATCCTGGAATTGTCTTGACACTGACTTTATGAGGAGACTGATTCTCATGCCATAAAGAACAGAACCCCACCTCCAGGGACTTGGAAGGGGCTCTGTAGGCCAAGATTAGTTTCCAGTGGTTTGGGAAGGGATGGAAAAGACTCCGTTAGTACCTTTCTGCCCTTGCCCAGCCAGTTTGGCTCAAGGCAGAGCCTGCTGCTTCGTGTAGCAGTTCCAGGTGGCCCATTTCTTCTTCCGGGGGCGGGGGGATCAGGGAGTCCTCTTCTGTGTTCAAGGCCCAGTGGCTCCAGGCCTCTGCAAGTCGCCCACGCGGGCTAGGAACAAAGTCCAGTGTGTTGCTCCTGCCGCCGCCGTAACCATGCCACCCACCTGCCCGGCTCTGCCCTCCGCCTCCCTAAACCCATCTGCCATACAGTGTAGGCTTCTTCCTCTTTCCTTTGTTCCCACTGACTGCAGTTCCACCTTTGGTTGCGGGGACCCTGTGGCCAGAGCTGAACTATAATGGTGCAGGAAGGGGAATGACTTCAAGTGGAACTCTGGAACCACATGGCTCTCCTCTTAACACAGAGAAGCTGACCTTGGCCACAGATGGATTTTCAGGGATAAAGAACAGGACATGGGGCCGGGTGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGGGCGGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTAACACCGTCAAACCCCATCTCTACTAAAAATACAAAAAATTAGCCAGGCGTGGTGGCGGGCACCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCAGGAGGCAGAGCTTGCAGTGAGCCAAGATCGCGCTACTGCACTCCAGCCTGGGTGACAGATCGAGACTCCGTCTCAAAAAAAAAAAAGAACAGGACATGGGAATGACCTTGGCTTGTTTTTGGCAGTAGCCCAGGTTTCTAGAGAATCTTGGCTTCTCCAGTGATGGGTTGGGTTTTTTCAAAGAAGCGTCTGGGAAACGTTGGGCTGCAGAGCATTTGCGGATGAGATTCAAAGCGTCCCATCTGTGGCCACCTCTGATGCTGGCACCGTCGAGGGGCATGAGTGAAGCCATCTTTCCAGGGTGCTGTTTGGGTCATGATTTAACTTTACTAAACCTCCTGAGTCTGTATTGGGGGAAGGTGGCCTGCAGTGTCCCCGGCTCCTCAGCCTGGCATCAAAACCTCCTGTGATCTCACCCTGCAGGCCCAGACTCTCTTTTGCTAGGAAGCCTCCCTGTGCAGCCAGCCCTTAGGGGTCCCTTCTTCCTTCGTGCTCAAGTCACTACTGCCTGCCCAACCCCATTCATTTGGCATTTATGTGACTGTAACTGTGTTCTGTGTGCCCCTCTAGTCTTCAGACCTCTTGAGGAGAGGCTACTGTGTCTTTGTATCCTACAGAGCACTCAGCACAGTTGAAAAGCTCTTCAGGGGGCTGGGCGTGGTGGCTCACGCCTGTAATCCTAGCACTTTGGGAGGCCGAGGTGGGCAGATCACCTGAGGTCAGGGGTTCGAGACCAGCCTGGCCAACATGGCGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCTGGGTGTGGTGGCTCATGCCTATAATCCCAGCTACTCGAGAGGCTGAGGCAGAAGAATCACTTGAACCCAGGAGGTGGAGGTTGCAGTGAGCTGAGATCACACCACCACATTCCAACCTGGGTGACAAGAGCAAAAAAAGAAAAAGAAATCTCAAAAAGAAAAAGAAAAGTTTTTCAGGGGACTCTTGCAATTGTGGCATCATGATCCTTCACACCTGCACTTACTGGACGCCTCCTGTGTACACAGCCCCTTTCCTCCTGTGCACAAGGTGGCCTGTGTTAGGACAGAGGCTGCAGCTCCCCCTGACCCTGCCTCTCCCCTGTTTGCATCTACAGGTACCCAGACAGCCATTGTCTTCATCAAGCAGCCGTCCTCCCAGGATGCACTGCAGGGGCGCCGGGCGCTGCTTCGCTGTGAGGTTGAGGCTCCGGGCCCGGTACATGTGTACTGGCTGCTCGATGGGGCCCCTGTCCAGGACACGGAGCGGCGTTTCGCCCAGGGCAGCAGCCTGAGCTTTGCAGCTGTGGACCGGCTGCAGGACTCTGGCACCTTCCAGTGTGTGGCTCGGGATGATGTCACTGGAGAAGAAGCCCGCAGTGCCAACGCCTCCTTCAACATCAAATGTGAGAGCCAGGGGGGCTGTGCCCAGTCCCCCTGTCAGACCCTCAATGACTGAGGCCTGGGGGATCCCTCCCTTACCTCAGCTTCTCCCATTTCCAGTTAAACGGGCCAGGCAGAATGCATTTATCATCAGCTTTTTTTGCTCATGTGGATAAGAGGAAATTAAAAGTTATATTTTTTCCAAAATTTTTTCCTTCAAGTCTGGGACCCATTTATCTGCTGCATGCTTGTGCAAATGGAAAGGGCGGCCGAGCCCTTGGCCAAGTGTCAGACTTGACCTGCCAGGGACCAGGCAGGCACTTAGTATCTGGTAACTGTAGCCCCAGCCTCAGCCTCCAGGGCTTCCTTGTGTCTGTTGGCACAGAGCCTCGAGGTTCCACGGCTTCCTGCTTGTCCTCCTTGCCCTCTGCCTTCCCGCCTTTGCCCTGCTCTGCCCCTCACTGCAACCGTGGCCTCTGCTACAGGGATTGAGGCAGGTCCTGTGGTCCTGAAGCATCCAGCCTCGGAAGCTGAGATCCAGCCACAGACCCAGGTCACACTTCGTTGCCACATTGATGGGCACCCTCGGTAAGGAGTCAGGGAGGTGGGGATGAAGAGGGTTATTCCGGACAGGGATGTCTCCCCAAATCTTGGACTCTATGCGGATGTTACCTCGCTCCATTCTGTGACCACTCGTTCCACCACCACAGTGCTCTTCACCCTGCCCTCCCCCAGATATTGCCCTATGCCGGCCCCTGGTGGCCTGAAGAGTGAGCCACATTGTGTGTAGAGCTGGGAGATCTAATATCAACTTGATTCTGCCCCTGAATCAGAATTACTGTGTGCCCTTTTGCAAGTCCCTTCCCTTCCTCAGGCCGTTTCTCCATGTATAAACTGAAAGGTCTAGCCCTGACCCTCTGATACTGTATCCTCTCCACTGGCCACTGAGTACCCCTCCCCACCACTGCTGACTGTGTCTGCCCTGCAGGCCCACCTACCAATGGTTCCGAGATGGGACCCCCCTTTCTGATGGTCAGAGCAACCACACAGTCAGCAGCAAGGAGCGGAACCTGACGCTCCGGCCAGCTGGTCCTGAGCATAGTGGGCTGTATTCCTGCTGCGCCCACAGTGCTTTTGGCCAGGCTTGCAGCAGCCAGAACTTCACCTTGAGCATTGCTGGTGAGCCTGGGGTGGGGGCGGAAGGGATGAGGTGAGCACAGGAGGGCCTCACCACCCAGGCTGCATGCTCCCCATCTTTCCCTCTCCAGATGAAAGCTTTGCCAGGGTGGTGCTGGCACCCAGGACGTGGTAGTAGCGAGGTATGAGGAGGCCATGTTCCATTGCCAGTTCTCAGCCCAGCCACCCCCGAGCCTGCAGTGGCTCTTTGAGGATGAGACTCCCATCACTAACCGCAGTCGGTAAGGCATCTGGCTGGGAGCATTCCAGTACCATGTACCACACACATGCATTCTGTAGAAGGGGGTGCGATTTGTATCACAGACATCACAGATTTGTACATGTATTATTTCAAATTTCCAAGAGACACAGTTGAATGTTTTGCTTTTACAAGATGAGTCTGACACAGGGAAGAGTCTCTGGGAAGAGCCCTTTTCCAGTTTGCACAAAGGCACTCTGAGCTAGTGATGGTTCCACTTCTCTCTCCCCACCACCCACATGGGTCTTCTGGGATGGAAGGGTAGAGACCCCAGGGATGAGAGAGACACAAGAGACCTAGGTAGTCAGGTGGTCATAGTCTAGATAGCGTGATCCAGTGTGGCAAAGACATCACACACACACACACACACACACACACACACACACACACACACACACACTTTTTAGAGGAAGCAAACACCTCTAGAAGGTTCCAGCCCTACTGCAGGGACTTCTGTTCTTGCCTGGACCCTAGGAGAACATAGAACACCTTTGCAGTGTCAGGCACGACCATGGTCCTTGGGGGTGTGGCTTCCTAGGCTAGGGGCTTGGATGCCACTTGGCTTCAGCCCTGAGGACATCATCAGTAGCCTGCCTGGTATGGCTGAGGGGTATCTGACCCTGGAGCTGGGTGCCCTATCAACGTGGCCCTGTGGGAAGGTGGCTTCCCTGTCTTGACAGTTGCACATGGTGGTTATTCTGTGTATTAGTCCATTTTAATGCTGCTGATAAAGACATACCCGAGACTGAGCAGTTTACAAAAGAAAGAGGTTTAATTGGACTTACAGTTCCACATGGCTGGGCAAGCCTCACAATCATGGCAGAAGGCAAGGAAGACCAAGTCACGTCTTACATGGATGGCAGCAGGCAAAGGAGAGAGAGCTTCTGCAGGGGAATGCCTCATTTTAAAACCATCAGATCTCGTGAGACTTGTTCACTATCATGAGAACAGCACGGGAAAGACTTGCCCCCATGATTCAGTTACCTCCCACTGGGTCCCTCCCACAACATGTGGGAATTATGGGAGCTACAATTTAAGATGAGATTTGAGTGGGGACACAGCCAAACCATATCACTCTCTCTCGCACCTGCATTGCCATTTGCCTCATTGCTTCCCGAGCTGATCTCCTGTAGTTGGAACACACACCTGCACGTGCACCTGAGCAAGTGTATGCAGGCACACACACCAGTCTGGAGTGTCAGCTTCCTTCTCTCTGCCCTGTTCCTGGTCGATTCCTTACTACATTTCCGACTTTGCAGGAAAGGGGTTCCCTTGCATTCCTTTCCAGAACTAGGCCTATTGGCCACTTTCTCCAAATTGCACTCTCCCCTCTGCAGCCCCCCACACCTCCGCAGAGCCACAGTGTTTGCCAACGGGTCTCTGCTGCTGACCCAGGTCCGGCCACGCAATGCAGGGATCTACCGCTGCATTGGCCAGGGGCAGAGGGGCCCACCCATCATCCTGGAAGCCACACTTCACCTAGCAGGTGAGTCTCTGGGTCTGGGGTGCTGATGTGGGAGGCTGCCTTTAACATTTTTGGCACATAGAGATTTAGGCTTCTGTTTTTACTCAGCCGCTTGGAGGGCAGGGGAAGAAAAGGATGCTGGGAGTAGGAAGAGGATATGCAGAGGGCTGCAGGGGAGGGTTTGTGCTGGGAAAGGGCCTAGGCTTGCTGTGGGAGAACATCATGTACCTGAGACCCACAATAATTGGGCCATTCCTCCTACTTATGTCCTTGCAGAGATTGAAGACATGCCGCTATTTGAGCCACGGGTGTTTACAGCTGGCAGCGAGGAGCGTGTGACCTGCCTTCCCCCCAAGGGTCTGCCAGAGCCCAGCGTGTGGTGGGAGCACGCGGGAGTCCGGCTGCCCACCCATGGCAGGGTCTACCAGAAGGGCCACGAGCTGGTGTTGGCCAATATTGCTGAAAGTGATGCTGGTGTCTACACCTGCCACGCGGCCAACCTGGCTGGTCAGCGGAGACAGGATGTCAACATCACTGTGGCCAGTGAGCACCTTTGCCCTGAAGGTCAAGGAGAGGTGGAGGGGAACACAGGTCTGGGTGTGATGGACAGAGGCTTCCCTGGTACTCACCTGAGGTCCTCTCAGTTCTGGGCCCTGCAGGCTTGGGAATCAGTTCACTACTGGGAGTCGGTGTAGACAGTAGAGAGCCAGGCACAGGGGTTAGGGTGGGTGCTCCAGAGGAAATAGTGACAGCCCTCACAGAAAAATGGTCCTCTGGGTACAGAGGAAAGAGCAATAGACTAGGAATCTAGAGACCTGGGTCCCACTATGCTCCCAATGCACAGAGTGACGATGGTAGCAAATCACCCACCTTACTGGGGTTCAGTCTCCTCATCCATAAAATGGAGCCAGTAATCTACTACCTACCCCACAAGGTGGTTGGAAAGGTCAAATGAAGTAGATGAAAATGATTTTTCTTTTTTTCTTTTTTGGGAGGTAATAGTGGACAAAATTATAGGTGATTTTTAATTTGTTTTAGTTTGTTATGCTTTCCAAATTTCTTTACTAAACATATTACTTATGAAAAAAAAGAGGGGGAGAAAAGCAAGACATATGACTGAAAGTACACATGATAAACCCTTTATATCAGGCTGGGCGCGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGCCGGGCGGATCACAAGGTCAGGAGTTGGAGACCAGCCTGACCAACATGGTGAAACCCCATCTCTACTAAAAAATACAAAAATTAGCTGGGCGTGGTGGGGCACACCTGTAATCCCAGCTACTCAGGAGGCTGAGGGAGGAAAATTGCTTGAACCCAGGAGGCAGAGGTTGCAATGAGCTGAGATCGCACCGTTGCTCTTCAGCCCGGGCAACAGAGTGAGACTCCATCTCAAAACAAAACAAAACAAACCTTATATCCTCACTACAGGCATAGGGATTTATTTGTTTACTTATTTCTTTATACCATGCCTTGTTCCAAACCTTTTGTGGTAGTTTAAAAGGATACACAATAAAAATTACAATATGCACACAGAGACACACATACATACAAACTATTTTGCAATCTGCTTCTTCCACTTACCTTTATGTTACTATTATCTTTCCGAGTGGGTAACTTTGCAAAAAGATTTGAATATGAAAGTGGCTTTGTAAATGTGAAGTTGTGATGCTAGTATTCCCATTAGGATACTGAAATCTTAGAAGACATGCACCTATACTCAGGCCGTGATTTGGGTGGGAATCACAGCACATGTGTAACGAGTGCTCAGGACACTCACAGTGCAGGTGCAAAGGCAAGATGCACAGTCAAGCCTTCCTCCAGTTGAGGTCTGAGAATCACCTGCTTTCATTAGGGATACTTTTTCTTCTTTTGTTTGAGACGGAGTCTCACTCTGTCACCCAGGCTGGAGTGTAGTGGTGTGATCTTGGCTCACTGCAACCTGCGCCTCCCAGGTTCAAGTGATTCTCGTGCCTCAGCCTCCCAAGTAGCTGGGTTTAAAGGTGTGTGCCACCACACCTGGCTAATTTTTGTATTTTTAGTAGAGGCGATGTTTTACCATGTTGGCCAGGCTAGTCTCAAACTCTTGACCTCAAGTGATCCACCTGCCTCGGCCTCCTAAAGTGCTGGGATTATGGGTGTGAGCTGCCATGCCCGGCCTCATTAGGGATACTTTTTAAAACACAGGCCAGGCGTGGTGGTTCACACCTATAATCCCAGTGCTTTGGGAGGCCGAGATAGGAAGATCTCTTGAGCCCAGGAGTTTGAGACCAGGCTGGACAAAATAGTGAGACCTTGTGTCTACAGAAATTTAAAAAGTTAGCTGGGCGCGGTGGCTCACGCTTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGTGGATCACGAGGTCAGGAGTTCGAGATCAGCCTGACCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCTGGGCATGTTGGCACTCACCTGTAATCCCAGCTACTTGGGAGGCTGGGGCAGGAGAATTGCTTGAATCCAGGAGGCAGAGGTTGCAGTGAGTCGAGATCGTGCCATTGCACTCCAGCCTGGGTGACAGAGCGAGACTTTGTCTCAGGAAAAAAAAAAAAAAAAATTTAGCCAGGTATGGTGATGCACACCTATAATCCCAACTATTTGGGAGGCTGAGGCTGGAGGATCATTTGAGCTCGGGAGCTGGAGGTTCCAGTGAGCTATGATCACACTACTGTACTGGAGCCTGGATGACAGCAAGACCCTGTCTCCAAAAAAATGTCTAAGACATTGCTGGAGCCTTGAAAAAAAGAAAAAAAATGCAGAATTCGGGGCCCCACTCCACAGCTTCCAACCCAGGCTCTAGGGGTGGGACCCAGGTAGATGTGTATTTAACAAGTGCTCTGGGTGATACTTCATGTGTGTTCAAGTTGGGGAACTCTGGCTTTGATCAGTCATTCCACAGATGCTGACTGAGGCTGACACTGAGCCATGCCAGTGTCCCTTGCTGTGCTCTGAGAAGGAGGCCTGTGGCATTTGAGAGTGTGTGATGCAAACAGGGCCAGATCAGGTGCTGGGTGTTAGAGGGTAGAGCCCAGAAGACGGAGAGCTCTGGGAATGCATTCAGTCAACAGAGATTTGTCGAGCACCTTGTGCTAGCTCTTCCCAGTACTGGATGCAACTGTGAACAAAGCAGGCAGAAGCCCCTCTCCTTGTGGAGTTCATATTCTAACAGCGGGGACAAAGCCAGTAAGAAAAACTATCAAATGAACAAATATAATTTTAGGTGCCAGTAGAACAAGGGAGAACAATGAAATAGGGCAAGTGATAGAGAAAGGGCAGGGCCAGTGAGAGTTTAGGGAAGACTTCTGTAAAAGGTGCATTGGAGTTGGTATTTAAATTAAGAAGGGAGCCAGCCATGTGATAGGCCCTGAGATGGAAATGGGCTTGTATGTTGGAGGAACAGTAGTCGCCTGTGTGGCTTGAGTTGAATAAACGATGGTGAGAGGGGGTTGAAGTTGCCTGAGAGGTGGAAACTAAATCCCATAGGCCTTGTAAAAGCATAGTAAGGACTTTGGCTTCGGCATCAGTTATCCATTGCTACATAGCAAACCACTTCAAAACGTGGTGGCACTTTGGGAGGCCGAGGCAGGTGGATCACTTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCCCATCTCTACTAAAAATACAAAAATGGCTGGGTGCAGTGGCTCACGCCTTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGTGGATCACGAGGTCAGGAATTCGAGACCAGCCTGACCAACGTGCTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGTGTGGTGGCATGTGCCTGTAATCCCAGCTACTCAGGAGGCGGAGGTTGCAGTGAGCCGAGATTGCGCCACTGCACTCCAGCCTGGGCGACAGAGCGAGACTCTATCTCAAAATAAAATAAAAAATACAAAAAAAAAAATTATCCAGGCATGGTGGCACATGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAGGTGGAGGTTGCAGTGAACAGAGATCGCGCCACTGCACTCCAGCCTGGGTGACAGAGCAAGACTCCAACTCAAAAAAAAAAAAAACCAAACCGTAGTGACTTGAAACAACAAACAACAGCCGTTGACATGCTTATGATTCTGTGGACAGGTTCAGGTGGGCCCGCTCGTCTACCTGGTGTGCTATCGGCTGGGTGTACACGTTTGCAGTCAGCTGATGGGTGAGCTGGCCTGGTCCCTGGCGGCCACACTCACCTGTCTGGCAGTTGACTGGGGCTATTGACCAGGGTGCCTTAGTTCTCCTCCACATGGCATCTCCAGTAGGCTAGCCAGGCTTCCTCACCTGGCAGCTGGGTTCCGAGAGGGCAAAAGTGGAAGCTGCAGTACTACCACTGTGTTCTGTTGGACAAGGGAAGTCACAGGCCACTCAGATTCCAGGGACTAGAAAAATACACCATGACCCAGTTCAGTGGCTCACGCTGCAATCCCAGTATTTTAGGAGGCCAAGGTGGGAGGACTGCTTGAGCCTAGGAGTTTGAGACCAGCCTGGACCACATGGCCAGACCCCGTCTCTACTAAAAATAAAAAAAAATTAGCTGGGCCTGGTGGTACGCGCCTGTGGTCCTAGCCACTTTGGAGGCTGAGGTGAGAGGGTTGCTTGAGCCTGGGACGCTGAGGCTGCAGTAAGCCATAGTGGCGTGACTGCACTCCAGCCTAGGTAACAGGATGAGACCCTGTCTCACACACACAAAAAGAAATAAACTCTACCTCTTGATGGGAAAGTGTCAACATCACATTGAAAAGAGTTGAAAGGGATGGGAGGATTGTTTATAACTATCATTGCAAGCAGCCTACCACACCACATTGGATGGGAAGGTCTTGCAGGGTTTTGAGTGGAAGACTGATTTTTCAGAAGATCGCTCTGGCTGTTTTGAGACTAGAATGTTAGGGCCAAGAGTATAAACAAGGAGACCCATACTAAGTAGATGACTATAATAGTCTAGGGGAGATACTGAAGGAGCTTGAACAGAGGCAGTAGCGGTGGGGGTGAGAGGTGGTCAAATTCAGGATATATTTAGGAGCCCCAGTAGATAGGGTTTGGTAGAACGAGTGTGATGGGGGTGGGGTATAGAGAGAAGACAAGGCTAGCTTTTTGGCTTTCTTAACTGACTAGATAGTTCTATTGACTGAGATAGGGAAAATGAGTGAGCAGGTTTTGGGGGAAGTCAGGTGTTCTCTTTAGCACACGTAAAGTTTGAGATGCCTGTTGGATATCCAGTTGGAAATGCCAGTAGGCAATTGGATTTATAGGAATCAGGATGTAGTTTTGGGAACCAACCATTCATGTTGTATTGAAGGTTGTGGACTGGATGAGTGAGTGTTGCTAAAACAGAACAGATTTCTGAGGACTGCGCCTTGGCATTTTATGGTGTAATAAGAAGAACAAGGGTTTCAGAGTCATATAGACCAGAGTTTTAAATTCTGTCACTATTAGGTATTAGCAAATCACCATTACTTCTTCTTTTTTTATTATTGTTATTATTATTTTTTATCTTTTATTTGAGATGGAGTCTCACTCTTTCGCCCAGGCTGGAGTGCAGTGGCGTGATCTCGGCTCACTGCAACCTCCACCTCCCAGGTTCAAGCGATTCTTGTGCCTCAGCCTCCCCAGTAGCTGGGATTACAGGTGCCCGCCACCACACCCGGCCAATGTTTTTGTATTTTTAGTAGAGACGGGGTTTCACTATGTTGGCCAGACTGGTTTTGAACTCCTGACCTCAAATGATCTGCCCGCCTTTGCCTCTTAAAGTGCTGGGATTACAGGCGTGAGCCACTGTGCCTGGCCACCATTACTTCTTTGAACATCTGTTTTCTTAGCTGTAAAGTGCGTGTTGGAATACATACCTTGGGGGGTTTGTTTTTTTTGTTTTTTTTAGAGATGGGGTTTCACTCTGTTGCCTAGGCTAGAGTGCAGTGTCTTACAGCAGCCCTCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACCACTCCCTGCCTACCATAGGGGCTTATAACTAAGATAATGTTAACTAAGTTAATTAATGTGACAGTTAACTAAGATAATGTTCAAAAGGTGCTGGTGGCCGGGCGTGGTGGCTCATGCCTGTAATCTTAGCACTTGGGGAGGCTTGAAGCCAGGAGTTTGAGACCAGCCTGGGCAACATAGCAAGACCCCATCACTACAGAAAATAAAAAATTAGCCAAGTATTGTGGTGCATGCCTGTGGTCCCAGCTATTTGGGAGGCTGAGGTGGGAAAAATCACTTGAGCCTATGAGTTCGAGACTGTAGTGAGCCATAATTGTGTCACTCTACTCCAGCCTGGGTGACAGAGTGAGAACCTGTCTTAAAAGAAAAAAAATTTTAAAAAAGGTGCTGGCACCTGGGTCTTCCGTAAAGGGAAACTCCTGCCATCTGAGGAGCATGGGGCTTCTGAACTCAGGGCCTAGAATGGTAGTAGGATTTCTTGGAGATGGATCCTTTAGTTTTGGAGACCTGTGAAGCAGCCTTCACTGTTTCCTTCCTGTCTGTCTAGCTGTGCCCTCCTGGCTGAAGAAGCCCCAAGACAGCCAGCTGGAGGAGGGCAAACCCGGCTACTTGGATTGCCTGACCCAGGCCACACCAAAACCTACAGTTGTCTGGTACAGAAACCAGATGCTCATCTCAGAGGTGAGAAAGAAGAGTGTTGCTAGTGGATGGGCGGGGCCTTCCTCCACTTGCCCTCTTCTGTGCTCACCTCCATAGCACTCTTACCCTGGAGGCAGCCTCCAGGGAGAGGTGGGTGTGGGT"; + final boolean isNegativeStrand = false; + + // Set expectedPositions = new HashSet<>(Arrays.asList(23, 26, 36, 45, 70, 109, 197, 225, 240, 255, 285, 296, 312, 347, 366, 398, 436, 577, 650, 652, 717, 749, 841, 932, 983, 1005, 1011, 1073, 1195, 1253, 1411, 1451, 1472, 1497, 1503, 1540, 1594, 1605, 1734, 1743, 1748, 1767, 1780, 1789, 1868, 1910, 1929, 1947, 1982, 1989, 2018, 2039, 2051, 2063, 2068, 2080, 2092, 2113, 2141, 2155, 2164, 2183, 2191, 2196, 2221, 2223, 2250, 2422, 2430, 2486, 2624, 2653, 2751, 2780, 2801, 2855, 2934, 2977, 3003, 3030, 3056, 3105, 3107, 3130, 3163, 3225, 3246, 3308, 3396, 3399, 3477, 3514, 3532, 3544, 3589, 3633, 3651, 3681, 3702, 3737, 3763, 3796, 3800, 3802, 3833, 3836, 3904, 3907, 3943, 3977, 3997, 4007, 4036, 4046, 4063, 4087, 4115, 4125, 4130, 4190, 4194, 4257, 4261, 4350, 4371, 4423, 4446, 4455, 4521, 4640, 4652, 4667, 4707, 4732, 4735, 4795, 4834, 4853, 4899, 4924, 4941, 4960, 4980, 4995, 5007, 5016, 5040, 5061, 5086, 5095, 5097, 5106, 5139, 5145, 5148, 5161, 5189, 5199, 5209, 5229, 5236, 5282, 5328, 5353, 5400, 5422, 5459, 5465, 5517, 5524, 5529, 5558, 5573, 5585, 5587, 5592, 5611, 5614, 5655, 5674, 5697, 5704, 5707, 5711, 5717, 5734, 5751, 5788, 5790, 5845, 5866, 5888, 5899, 5914, 5916, 5920, 5933, 5937, 5950, 5967, 5969, 5974, 5976, 5981, 6004, 6015, 6024, 6027, 6029, 6032, 6050, 6071, 6082, 6099, 6102, 6106, 6130, 6132, 6135, 6141, 6148, 6153, 6157, 6159, 6165, 6168, 6194, 6200, 6208, 6225, 6228, 6235, 6239, 6242, 6252, 6259, 6261, 6278, 6283, 6285, 6291, 6319, 6322, 6344, 6357, 6363, 6379, 6386, 6388, 6390, 6394, 6396, 6401, 6405, 6419, 6421, 6423, 6425, 6450, 6459, 6462, 6473, 6480, 6488, 6498, 6501, 6507, 6512, 6533, 6535, 6537, 6539, 6547, 6552, 6560, 6566, 6570, 6574, 6585, 6596, 6605, 6609, 6612, 6619, 6631, 6636, 6642, 6646, 6657, 6661, 6668, 6676, 6682, 6687, 6693, 6699, 6706, 6711, 6717, 6721, 6730, 6745, 6751, 6756, 6760, 6765, 6771, 6775, 6778, 6781, 6784, 6795, 6799, 6824, 6826, 6846, 6850, 6853, 6856, 6859, 6862, 6868, 6883, 6886, 6892, 6894, 6898, 6904, 6966, 7007, 7020, 7029, 7045, 7053, 7060, 7063, 7071, 7090, 7116, 7128, 7161, 7171, 7173, 7193, 7228, 7236, 7248, 7253, 7272, 7274, 7283, 7288, 7306, 7312, 7352, 7359, 7364, 7371, 7380, 7386, 7397, 7407, 7419, 7426, 7455, 7463, 7474, 7480, 7482, 7487, 7491, 7497, 7517, 7519, 7521, 7523, 7548, 7551, 7555, 7582, 7588, 7597, 7606, 7610, 7625, 7629, 7639, 7644, 7672, 7676, 7681, 7688, 7709, 7713, 7723, 7729, 7739, 7744, 7753, 7763, 7770, 7772, 7820, 7822, 7829, 7868, 7878, 7900, 7938, 7943, 7950, 7972, 7975, 7980, 7984, 7990, 8002, 8011, 8029, 8031, 8040, 8048, 8072, 8091, 8093, 8100, 8117, 8146, 8154, 8169, 8185, 8189, 8194, 8233, 8253, 8321, 8338, 8345, 8349, 8352, 8358, 8360, 8366, 8382, 8386, 8396, 8402, 8425, 8435, 8439, 8444, 8462, 8469, 8471, 8483, 8490, 8509, 8517, 8523, 8549, 8551, 8555, 8566, 8569, 8579, 8591, 8594, 8624, 8627, 8632, 8645, 8648, 8667, 8673, 8697, 8708, 8721, 8752, 8757, 8763, 8780, 8794, 8799, 8805, 8839, 8843, 8848, 8855, 8859, 8865, 8885, 8907, 8915, 8926, 8940, 8948, 8958, 8967, 8972, 8981, 8983, 8988, 8990, 8993, 8996, 9006, 9016, 9020, 9047, 9050, 9076, 9096, 9100, 9140, 9144, 9166, 9174, 9184, 9188, 9194, 9224, 9249, 9295, 9303, 9327, 9419, 9426, 9443, 9478, 9480, 9490, 9500, 9506, 9508, 9511, 9521, 9554, 9559, 9561, 9570, 9585, 9593, 9653, 9668, 9672, 9689, 9695, 9708, 9710, 9736, 9745, 9749, 9754, 9758, 9784, 9789, 9796, 9802, 9808, 9814, 9820, 9828, 9844, 9848, 9866, 9881, 9920, 9925, 9933, 9949, 9961, 10083, 10277, 10407, 10458, 10474, 10538, 10560, 10568, 10621, 10627, 10665, 10713, 10731, 10739, 10760, 10938, 11111, 11152, 11336, 11365, 11406, 11417, 11422, 11597, 11634, 11858, 11938, 11974, 12071, 12204, 12222, 12282, 12297, 12337, 12353, 12383, 12400, 12407, 12431, 12452, 12458, 12462, 12474, 12504, 12515, 12535, 12593, 12603, 12605, 12610, 12748, 12966, 12982, 13049, 13055, 13058, 13068, 13076, 13091, 13119, 13127, 13242, 13250, 13305, 13322, 13339, 13364, 13376, 13409, 13417, 13422, 13453, 13464, 13469, 13497, 13509, 13513, 13574, 13645, 13648, 13680, 13688, 13722, 13754, 13786, 13814, 13824, 13952, 13996, 14026, 14039, 14371, 14377, 14403, 14409, 14430, 14445, 14460, 14472, 14479, 14496, 14504, 14520, 14546, 14552, 14566, 14575, 14583, 14599, 14614, 14636, 14646, 14684, 14698, 14705, 14727, 14731, 14751, 14771, 14774, 14783, 14785, 14795, 14820, 14833, 14850, 14856, 14880, 14901, 14938, 14942, 14967, 15065, 15128, 15200, 15213, 15232, 15238, 15246, 15261, 15280, 15299, 15357, 15365, 15396, 15403, 15452, 15521, 15549, 15559, 15573, 15584, 15592, 15616, 15629, 15631, 15639, 15643, 15650, 15687, 15692, 15727, 15769, 15856, 15879, 15886, 15893, 16045, 16091, 16137, 16160, 16207, 16229, 16235, 16278, 16292, 16321, 16326, 16359, 16372, 16382, 16384, 16403, 16417, 16425, 16443, 16458, 16464, 16469, 16471, 16491, 16495, 16512, 16567, 16588, 16601, 16625, 16628, 16646, 16666, 16682, 16690, 16709, 16730, 16761, 16787, 16806, 16815, 16833, 16837, 16842, 16852, 16859, 16864, 16866, 16870, 16888, 16894, 16908, 16930, 16940, 16968, 17095, 17159, 17220, 17339, 17366, 17374, 17518, 17708, 17807, 17817, 17839, 17860, 17928, 17956, 17958, 17970, 17995, 18001, 18021, 18147, 18238, 18377, 18428, 18537, 18547, 18555, 18577, 18591, 18681, 18704, 18738, 18740, 18750, 18761, 18797, 18800, 18815, 18821, 18964, 19249, 19281, 19287, 19308, 19314, 19332, 19347, 19375, 19400, 19409, 19416, 19455, 19460, 19468, 19579, 19583, 19587, 19633, 19878, 19964, 20007, 20012, 20209, 20241, 20288, 20305, 20307, 20326, 20332, 20373, 20393, 20406, 20464, 20496, 20532, 20560, 20601, 20630, 20638, 20656, 20688, 20730, 20783, 20798, 20840, 20925, 20929, 20947, 21005, 21143, 21147, 21153, 21235)); + + List modificationSets = BaseModificationUtils.getBaseModificationSets(mm, null, seq.getBytes(), isNegativeStrand); + assertEquals(1, modificationSets.size()); + + // All "Cs" should have a likelihood + int cCount = (int) seq.codePoints().filter(ch -> ch == 'C').count(); + + BaseModificationSet bms = modificationSets.get(0); + Map likelihoods = bms.getLikelihoods(); + assertEquals(cCount, likelihoods.size()); + + } } + From d7e71e4f8a1d04de7d3434208fd7bdc29de02f99 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 16 Aug 2023 23:55:53 -0700 Subject: [PATCH 084/116] fix unit test --- .../org/broad/igv/sam/mods/BaseModificationCountsTest.java | 5 +++-- 1 file changed, 3 insertions(+), 2 deletions(-) diff --git a/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java b/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java index 6b67c2d74f..05e0649164 100644 --- a/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java +++ b/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java @@ -35,8 +35,9 @@ public void incrementCounts() throws IOException { } assertTrue("No data retrieved: " + readCount, readCount > 0); - int[] expectedPositions = {13846181, 13846182, 13846227, 13846228, 13846232, 13846233, 13846234}; - int[] expectedCounts = {1, 1, 1, 2, 1, 1, 1 }; + // Positions and counts manually computed from alignment & MM tag + int[] expectedPositions = {13845691, 13845746, 13845752, 13845759}; + int[] expectedCounts = {1, 1, 1, 1 }; BaseModificationKey key = BaseModificationKey.getKey('C', '+', "m"); From c7489e3601101ca39b0ecce9782b6cefec382f11 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 17 Aug 2023 11:44:59 -0700 Subject: [PATCH 085/116] bug fix --- .../java/org/broad/igv/sam/mods/BaseModificationUtils.java | 2 +- .../org/broad/igv/sam/mods/BaseModificationCountsTest.java | 5 ++--- 2 files changed, 3 insertions(+), 4 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java index 072f236fc7..12fcc81075 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java @@ -151,8 +151,8 @@ public static List getBaseModificationSets(String mm, byte[ likelihoodMap.get(modification).put(position, likelihood); } } + matchCount++; } - matchCount++; } p++; } diff --git a/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java b/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java index 05e0649164..6b67c2d74f 100644 --- a/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java +++ b/src/test/java/org/broad/igv/sam/mods/BaseModificationCountsTest.java @@ -35,9 +35,8 @@ public void incrementCounts() throws IOException { } assertTrue("No data retrieved: " + readCount, readCount > 0); - // Positions and counts manually computed from alignment & MM tag - int[] expectedPositions = {13845691, 13845746, 13845752, 13845759}; - int[] expectedCounts = {1, 1, 1, 1 }; + int[] expectedPositions = {13846181, 13846182, 13846227, 13846228, 13846232, 13846233, 13846234}; + int[] expectedCounts = {1, 1, 1, 2, 1, 1, 1 }; BaseModificationKey key = BaseModificationKey.getKey('C', '+', "m"); From b454ad6afc59c3cd4331164b3692b8d91ad1037a Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 17 Aug 2023 12:20:29 -0700 Subject: [PATCH 086/116] "skipped bases" bug --- .../broad/igv/sam/mods/BaseModificationUtils.java | 13 +++++++------ 1 file changed, 7 insertions(+), 6 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java index 12fcc81075..53ff05a488 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java @@ -127,7 +127,7 @@ public static List getBaseModificationSets(String mm, byte[ if (base == 'N' || sequence[p] == base) { int position = isNegativeStrand ? sequence.length - 1 - p : p; - if (matchCount == skip && idx < tokens.length) { + if (matchCount == skip) { // && idx < tokens.length) { for (String modification : modifications) { byte likelihood = ml == null ? (byte) 255 : ml[mlIdx++]; likelihoodMap.get(modification).put(position, likelihood); @@ -136,16 +136,17 @@ public static List getBaseModificationSets(String mm, byte[ skip = Integer.parseInt(tokens[idx++]); matchCount = 0; } else { - if (!skippedBasesCalled) { - // If skipped bases are not called unmodified we are done. If they are we need - // to scan the entire sequence + if (skippedBasesCalled) { + // MM tag is exhausted, but continue scanning for skipped bases + skip = -1; + } else { + // If skipped bases are not called unmodified we are done. break; } } } else { if (skippedBasesCalled) { - // Skipped bases are assumed be called "modification present with 0% probability", - // i.e modification has been called to be not present (as opposed to unknown) + // Skipped bases => "modification present with 0% probability" for (String modification : modifications) { byte likelihood = 0; likelihoodMap.get(modification).put(position, likelihood); From f9a82e3a0bbd3f8ed759170052180f85de068739 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 17 Aug 2023 20:06:25 -0700 Subject: [PATCH 087/116] fix simplex modification calculation --- .../broad/igv/sam/AlignmentDataManager.java | 19 +++++++++---------- 1 file changed, 9 insertions(+), 10 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentDataManager.java b/src/main/java/org/broad/igv/sam/AlignmentDataManager.java index 222fe3a55f..fd377a704b 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentDataManager.java +++ b/src/main/java/org/broad/igv/sam/AlignmentDataManager.java @@ -47,6 +47,7 @@ import java.io.IOException; import java.util.*; +import java.util.stream.Collectors; import static org.broad.igv.prefs.Constants.*; @@ -419,18 +420,16 @@ private void updateBaseModfications(List alignments) { } } } + // Search for simplex modifications (single strand read, e.g. C+m with no G-m) - Map tmp = new HashMap<>(); + Set minusStranMods = allBaseModificationKeys.stream() + .filter(key -> key.getStrand() == '-') + .map(key -> key.getModification()) + .collect(Collectors.toSet()); for(BaseModificationKey key : allBaseModificationKeys) { - if(tmp.containsKey(key.getModification())) { - tmp.remove(key); - } else { - tmp.put(key.getModification(), key); - } - } - for(Map.Entry entries : tmp.entrySet()) { - simplexBaseModfications.add(entries.getKey()); - simplexBaseModfications.add("NONE_" + entries.getValue().getCanonicalBase()); + if(key.getStrand() == '+' && !minusStranMods.contains(key.getModification())) + simplexBaseModfications.add(key.getModification()); + simplexBaseModfications.add("NONE_" + key.getCanonicalBase()); // Mix of simplex & duplex keys for same base not supported. } } From e8c74e76c3ca0d384d11434b2f98090825c3b464 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 17 Aug 2023 22:15:02 -0700 Subject: [PATCH 088/116] bug fix -- alignment gap color not set if in squished mode --- src/main/java/org/broad/igv/sam/AlignmentRenderer.java | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java index b7927f0853..1f1f27ceaf 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java +++ b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java @@ -639,9 +639,11 @@ private void drawAlignment( gapGraphics.setColor(unknownGapColor); } else if (gap.getType() == SAMAlignment.SKIPPED_REGION) { gapGraphics.setColor(skippedColor); - } else if (h > 5) { - gapGraphics = context.getGraphics2D("THICK_STROKE"); + } else { gapGraphics.setColor(deletionColor); + if (h > 5) { + gapGraphics = context.getGraphics2D("THICK_STROKE"); + } } gapGraphics.drawLine(gapPxStart, y + h / 2, gapPxEnd, y + h / 2); From 3e0cd0cb08288995ae173ba8cfaefdcec171bd47 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 17 Aug 2023 22:15:34 -0700 Subject: [PATCH 089/116] basemods -- don't compute counts for soft-clipped bases --- .../igv/sam/mods/BaseModificationCounts.java | 27 ++++++++++++------- .../igv/sam/mods/BaseModificationUtils.java | 8 +++--- 2 files changed, 22 insertions(+), 13 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java index 980ede1b37..2c04c869a8 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationCounts.java @@ -53,6 +53,8 @@ public void incrementCounts(Alignment alignment) { for (AlignmentBlock block : alignment.getAlignmentBlocks()) { + if(block.isSoftClip()) continue; + // Loop through read sequence for this block for (int blockIdx = 0; blockIdx < block.getBases().length; blockIdx++) { @@ -162,7 +164,9 @@ public Set getAllModificationKeys() { } public String getValueString(int position, AlignmentTrack.ColorOption colorOption) { + StringBuffer buffer = new StringBuffer(); + StringBuffer nomodBuffer = new StringBuffer(); // /** // * Map for capturing modification likelihood "pileup", key is modification identifier, @@ -174,21 +178,26 @@ public String getValueString(int position, AlignmentTrack.ColorOption colorOptio buffer.append("
---------
"); buffer.append("Modifications with likelihood > " + (lastThreshold * 100) + "%"); - for (BaseModificationKey key : maxLikelihoods.keySet()) { - Map t = maxLikelihoods.get(key); - if (key.modification.startsWith("NONE_")) { - // continue; - } + final boolean includeNomods = colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR; + + Map> l = includeNomods ? nomodLikelihoods : maxLikelihoods; + for (BaseModificationKey key : l.keySet()) { + Map t = l.get(key); if (t.containsKey(position)) { - int count = this.getCount(position, key, lastThreshold, colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR); + int count = this.getCount(position, key, lastThreshold, includeNomods); if (count > 0) { - int likelihoodSum = getLikelihoodSum(position, key, lastThreshold, colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR); - int averageLikelihood = (int) ((((double) likelihoodSum) / count) * .3921568); + int likelihoodSum = getLikelihoodSum(position, key, lastThreshold, includeNomods); + int averageLikelihood = (int) ((((double) likelihoodSum) / count) * .3921568); //.39 => 100/255 String modName = BaseModificationUtils.modificationName(key.modification); - buffer.append("
  " + modName + " (" + key.base + key.strand + "): " + count + " @ average likelihood " + averageLikelihood + "%"); + if(key.modification.startsWith("NONE_")) { + nomodBuffer.append("
  " + modName + ": " + count + " @ average likelihood " + averageLikelihood + "%"); + } else { + buffer.append("
  " + modName + " (" + key.base + key.strand + "): " + count + " @ average likelihood " + averageLikelihood + "%"); + } } } } + buffer.append(nomodBuffer.toString()); return buffer.toString(); } } diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java index 53ff05a488..a66c684935 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationUtils.java @@ -36,10 +36,10 @@ public class BaseModificationUtils { codeValues.put("A", "Unknown A"); codeValues.put("G", "Unknown G"); codeValues.put("N", "Unknown"); - codeValues.put("NONE_C", "Unmodified"); - codeValues.put("NONE_T", "Unmodified"); - codeValues.put("NONE_G", "Unmodified"); - codeValues.put("NONE_A", "Unmodified"); + codeValues.put("NONE_C", "Unmodified C"); + codeValues.put("NONE_T", "Unmodified T"); + codeValues.put("NONE_G", "Unmodified G"); + codeValues.put("NONE_A", "Unmodified A"); } public static String modificationName(String modification) { From 9fb023d3b9a86967ad2c9e6229d1fdd16d8f8427 Mon Sep 17 00:00:00 2001 From: Kevin Lydon Date: Sat, 26 Aug 2023 18:31:11 -0400 Subject: [PATCH 090/116] Added nucleotide color options to the preferences menu (#1354) * Added nucleotide color options to the preferences menu * Modified SequenceRenderer to update nucleotide colors to match preferences when a new renderer is instanced --- .../org/broad/igv/renderer/SequenceRenderer.java | 2 +- .../resources/org/broad/igv/prefs/preferences.tab | 12 +++++++----- 2 files changed, 8 insertions(+), 6 deletions(-) diff --git a/src/main/java/org/broad/igv/renderer/SequenceRenderer.java b/src/main/java/org/broad/igv/renderer/SequenceRenderer.java index 5400abdcdd..5739fb589e 100644 --- a/src/main/java/org/broad/igv/renderer/SequenceRenderer.java +++ b/src/main/java/org/broad/igv/renderer/SequenceRenderer.java @@ -100,7 +100,7 @@ private synchronized static void setNucleotideColors() { public SequenceRenderer() { - if (nucleotideColors == null) setNucleotideColors(); + setNucleotideColors(); translatedSequenceDrawer = new TranslatedSequenceDrawer(); } diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index 294ed45a1f..e53c2799ea 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -25,6 +25,13 @@ DEFAULT_FONT_SIZE Default font size integer 10 SCALE_FONTS Scale fonts boolean FALSE Scale fonts for high resolution screens. Requires restart. ENABLE_ANTIALIASING Enable anti-aliasing boolean TRUE +##Nucleotide Colors +COLOR.A A color 0,150,0 +COLOR.C C color 0,0,255 +COLOR.T T color 255,0,0 +COLOR.G G color 209,113,5 +COLOR.N n color 128,128,128 + #Tracks IGV.chart.track.height Default numeric track height (pixels) integer 40 IGV.track.height Default feature track height (pixels) integer 15 @@ -353,11 +360,6 @@ CBIO_EXPRESSION_DOWN_THRESHOLD 1 DETAILS_BEHAVIOR CLICK SHOW_SIZE_WARNING TRUE SKIP_VERSION -COLOR.A 0,150,0 -COLOR.C 0,0,255 -COLOR.T 255,0,0 -COLOR.G 209,113,5 -COLOR.N 128,128,128 SASHIMI.SHOW_COVERAGE TRUE GENE_LIST_BED_FORMAT FALSE From f662ea62b431173a4c09f19b98a6c6c985125611 Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Sat, 26 Aug 2023 15:32:55 -0700 Subject: [PATCH 091/116] Expanded insertions (#1379) Redesign expanded insertion code. Fixes many bugs. Insertion markers are moved to the "ruler" area. Insertions can be closed by clicking on the expanded marker icon. --- .../broad/igv/sam/AlignmentDataManager.java | 87 +----- .../org/broad/igv/sam/AlignmentInterval.java | 72 +---- .../org/broad/igv/sam/AlignmentRenderer.java | 291 ++---------------- .../broad/igv/sam/AlignmentTileLoader.java | 2 +- .../org/broad/igv/sam/AlignmentTrack.java | 210 +++---------- .../org/broad/igv/sam/AlignmentTrackMenu.java | 8 - .../java/org/broad/igv/sam/BaseRenderer.java | 156 ++++++++++ .../java/org/broad/igv/sam/CoverageTrack.java | 3 +- .../org/broad/igv/sam/InsertionManager.java | 54 +--- .../org/broad/igv/sam/InsertionMarker.java | 17 +- .../broad/igv/sam/SpliceJunctionTrack.java | 3 +- .../java/org/broad/igv/session/Session.java | 18 +- .../org/broad/igv/track/RenderContext.java | 18 +- .../org/broad/igv/ui/panel/DataPanel.java | 4 +- .../broad/igv/ui/panel/DataPanelPainter.java | 104 ++++--- .../broad/igv/ui/panel/ReferenceFrame.java | 90 +++--- .../igv/ui/panel/RegionOfInterestPanel.java | 10 +- .../org/broad/igv/ui/panel/RulerPanel.java | 209 ++++++++----- .../org/broad/igv/prefs/preferences.tab | 6 +- .../igv/sam/AlignmentDataManagerTest.java | 2 +- .../broad/igv/sam/AlignmentPackerTest.java | 4 +- 21 files changed, 544 insertions(+), 824 deletions(-) create mode 100644 src/main/java/org/broad/igv/sam/BaseRenderer.java diff --git a/src/main/java/org/broad/igv/sam/AlignmentDataManager.java b/src/main/java/org/broad/igv/sam/AlignmentDataManager.java index fd377a704b..1aaba591d2 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentDataManager.java +++ b/src/main/java/org/broad/igv/sam/AlignmentDataManager.java @@ -230,10 +230,6 @@ public CoverageTrack getCoverageTrack() { return coverageTrack; } - public double getMinVisibleScale() { - return getVisibilityWindow() / 700; - } - public double getVisibilityWindow() { return getPreferences().getAsFloat(SAM_MAX_VISIBLE_RANGE) * 1000; } @@ -269,12 +265,13 @@ public AlignmentInterval getLoadedInterval(ReferenceFrame frame) { } public AlignmentInterval getLoadedInterval(ReferenceFrame frame, boolean includeOverlaps) { + // Search for interval completely containining reference frame region for (AlignmentInterval interval : intervalCache) { if (interval.contains(frame.getCurrentRange())) { return interval; } } - // No contains, look for overlap + // No interval contains entire region of frame, look for intervael with at least some overlap if (includeOverlaps) { for (AlignmentInterval interval : intervalCache) { if (interval.overlaps(frame.getCurrentRange())) { @@ -315,7 +312,7 @@ public void load(ReferenceFrame frame, AlignmentTrack.RenderOptions renderOptions, boolean expandEnds) { - if (frame.getChrName().equals(Globals.CHR_ALL) || frame.getScale() > getMinVisibleScale()) + if (frame.getChrName().equals(Globals.CHR_ALL) || (frame.getEnd() - frame.getOrigin()) > getVisibilityWindow()) return; // should not happen if (isLoaded(frame)) { @@ -458,10 +455,6 @@ private AlignmentTrack.ExperimentType getExperimentType() { return alignmentTrack == null ? null : alignmentTrack.getExperimentType(); } - private void setExperimentType(AlignmentTrack.ExperimentType type) { - if (alignmentTrack != null) alignmentTrack.setExperimentType(type); - } - public PackedAlignments getGroups(AlignmentInterval interval, AlignmentTrack.RenderOptions renderOptions) { //AlignmentInterval interval = getLoadedInterval(context.getReferenceFrame()); @@ -573,18 +566,10 @@ public void alleleThresholdChanged() { coverageTrack.setSnpThreshold(PreferencesManager.getPreferences().getAsFloat(SAM_ALLELE_THRESHOLD)); } - public boolean isTenX() { - return getLoader().isTenX(); - } - public boolean isPhased() { return getLoader().isPhased(); } - public boolean isMoleculo() { - return getLoader().isMoleculo(); - } - public Collection getLoadedIntervals() { return intervalCache; } @@ -629,71 +614,5 @@ public int getMaxReadCount() { } - static class IntervalCache { - - private int maxSize; - ArrayList intervals; - - public IntervalCache() { - this(1); - } - - public IntervalCache(int ms) { - this.maxSize = Math.max(1, ms); - intervals = new ArrayList<>(maxSize); - } - - void setMaxSize(int ms, List frames) { - this.maxSize = Math.max(1, ms); - if (intervals.size() > maxSize) { - // Reduce size. Try to keep intervals that cover frame ranges. This involves a linear search - // of potentially (intervals.size X frames.size) elements. Don't attempt if this number is too large - if (frames.size() * intervals.size() < 25) { - ArrayList tmp = new ArrayList<>(maxSize); - for (AlignmentInterval interval : intervals) { - if (tmp.size() == maxSize) break; - for (ReferenceFrame frame : frames) { - Range range = frame.getCurrentRange(); - if (interval.contains(range.getChr(), range.getStart(), range.getEnd())) { - tmp.add(interval); - break; - } - } - } - intervals = tmp; - } else { - intervals = new ArrayList(intervals.subList(0, maxSize)); - intervals.trimToSize(); - } - } - } - - public void add(AlignmentInterval interval) { - if (intervals.size() >= maxSize) { - intervals.remove(0); - } - intervals.add(interval); - } - - public AlignmentInterval getIntervalForRange(Range range) { - - for (AlignmentInterval interval : intervals) { - if (interval.contains(range.getChr(), range.getStart(), range.getEnd())) { - return interval; - } - } - - return null; - - } - - public Collection values() { - return intervals; - } - - public void clear() { - intervals.clear(); - } - } } diff --git a/src/main/java/org/broad/igv/sam/AlignmentInterval.java b/src/main/java/org/broad/igv/sam/AlignmentInterval.java index 72f0a358a0..05f05ae0d3 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentInterval.java +++ b/src/main/java/org/broad/igv/sam/AlignmentInterval.java @@ -45,6 +45,7 @@ public class AlignmentInterval extends Locus { private List alignments; private SpliceJunctionHelper spliceJunctionHelper; private List downsampledIntervals; + private PackedAlignments packedAlignments; public AlignmentInterval(String chr, int start, int end, @@ -99,7 +100,8 @@ static Alignment getFeatureContaining(List features, int right) { /** * Sort rows group by group - * @param option + * + * @param option * @param location */ public void sortRows(SortOption option, double location, String tag, boolean invertSort, Set priorityRecords) { @@ -111,11 +113,11 @@ public void sortRows(SortOption option, double location, String tag, boolean inv final int center = (int) location; byte referenceBase = this.getReference(center); - Comparator rowComparator = option.getComparator(center, referenceBase, tag, invertSort); + Comparator rowComparator = option.getComparator(center, referenceBase, tag, invertSort); - if( priorityRecords != null && !priorityRecords.isEmpty()){ + if (priorityRecords != null && !priorityRecords.isEmpty()) { rowComparator = Comparator.comparing((Row row) -> row.getAlignments().stream() - .anyMatch( aln -> priorityRecords.contains(aln.getReadName()))) + .anyMatch(aln -> priorityRecords.contains(aln.getReadName()))) .reversed() .thenComparing(rowComparator); } @@ -183,6 +185,7 @@ public List getDownsampledIntervals() { return downsampledIntervals; } + public SpliceJunctionHelper getSpliceJunctionHelper() { return this.spliceJunctionHelper; } @@ -207,65 +210,4 @@ public void dumpAlignments() { } - /** - * An alignment iterator that iterates over packed rows. Used for - * repacking. Using the iterator avoids the need to copy alignments - * from the rows - */ - static class AlignmentIterator implements Iterator { - - PriorityQueue rows; - Alignment nextAlignment; - - AlignmentIterator(Map> groupedAlignmentRows) { - rows = new PriorityQueue(5, new Comparator() { - - public int compare(Row o1, Row o2) { - return o1.getNextStartPos() - o2.getNextStartPos(); - } - }); - - for (List alignmentRows : groupedAlignmentRows.values()) { - for (Row r : alignmentRows) { - r.resetIdx(); - rows.add(r); - } - } - - advance(); - } - - public boolean hasNext() { - return nextAlignment != null; - } - - public Alignment next() { - Alignment tmp = nextAlignment; - if (tmp != null) { - advance(); - } - return tmp; - } - - private void advance() { - - nextAlignment = null; - Row nextRow = null; - while (nextAlignment == null && !rows.isEmpty()) { - while ((nextRow = rows.poll()) != null) { - if (nextRow.hasNext()) { - nextAlignment = nextRow.nextAlignment(); - break; - } - } - } - if (nextRow != null && nextAlignment != null) { - rows.add(nextRow); - } - } - - public void remove() { - // ignore - } - } } diff --git a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java index 1f1f27ceaf..3d9fa781c0 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java +++ b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java @@ -35,7 +35,6 @@ import org.broad.igv.prefs.IGVPreferences; import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.renderer.GraphicUtils; -import org.broad.igv.renderer.SequenceRenderer; import org.broad.igv.sam.AlignmentTrack.ColorOption; import org.broad.igv.sam.BisulfiteBaseInfo.DisplayStatus; import org.broad.igv.sam.mods.BaseModificationRenderer; @@ -58,8 +57,6 @@ import java.util.Map; import static org.broad.igv.prefs.Constants.SAM_ALLELE_THRESHOLD; -import static org.broad.igv.prefs.Constants.SAM_BASE_QUALITY_MAX; -import static org.broad.igv.prefs.Constants.SAM_BASE_QUALITY_MIN; import static org.broad.igv.prefs.Constants.SAM_CLIPPING_THRESHOLD; import static org.broad.igv.prefs.Constants.SAM_COLOR_A; import static org.broad.igv.prefs.Constants.SAM_COLOR_C; @@ -96,8 +93,8 @@ public class AlignmentRenderer { private static final Color OUTLINE_COLOR = new Color(185, 185, 185); //chimeric read connecting on the same contig colors - private static final Color INVERSION_COLOR = new Color( 200, 0, 0); - private static final Color NON_INVERSION_COLOR = new Color ( 230, 150, 150); + private static final Color INVERSION_COLOR = new Color(200, 0, 0); + private static final Color NON_INVERSION_COLOR = new Color(230, 150, 150); // Clipping colors private static final Color clippedColor = new Color(255, 20, 147); @@ -657,7 +654,6 @@ private void drawAlignment( y, h, gapPxEnd - gapPxStart - 2, - context.translateX, null); } } @@ -692,8 +688,15 @@ private void drawAlignment( int blockChromEnd = block.getStart() + block.getLength(); int blockPxStart = (int) Math.round((blockChromStart - bpStart) / locScale); int blockPxEnd = (int) Math.round((blockChromEnd - bpStart) / locScale); + + // Check if block is in visible rectangle + if (blockPxEnd < 0) { + continue; + } else if (blockPxStart > rowRect.x + rowRect.width) { + break; + } + boolean rightmost = blockIx + 1 == blocks.length; - boolean tallEnoughForArrow = h > 6; if (!rightmost) { // consider waiting to draw the block unless it is rightmost if (hideSmallIndelsBP && (blocks[blockIx + 1].getStart() - blockChromEnd) < indelThresholdBP) { @@ -716,11 +719,16 @@ private void drawAlignment( } } + boolean drawArrow = h > 6 && (leftmost && blockPxStart > 0 || rightmost && blockPxEnd < rowRect.x + rowRect.width); + blockPxStart = Math.max(0, blockPxStart); + blockPxEnd = Math.min(rowRect.x + rowRect.width, blockPxEnd); + // Draw block as a rectangle; use a pointed hexagon in terminal block to indicate strand. - int[] xPoly = {blockPxStart - (leftmost && alignment.isNegativeStrand() && tallEnoughForArrow ? arrowPxWidth : 0), + int[] xPoly = { + blockPxStart - (leftmost && alignment.isNegativeStrand() && drawArrow ? arrowPxWidth : 0), blockPxStart, blockPxEnd, - blockPxEnd + (rightmost && !alignment.isNegativeStrand() && tallEnoughForArrow ? arrowPxWidth : 0), + blockPxEnd + (rightmost && !alignment.isNegativeStrand() && drawArrow ? arrowPxWidth : 0), blockPxEnd, blockPxStart}, yPoly = {y + h / 2, y, y, y + h / 2, y + h, y + h}; @@ -766,11 +774,11 @@ private void drawAlignment( String chr = context.getChr(); final int start = block.getStart(); final int end = block.getEnd(); - if (end <= bpStart) { // block ends before the visible context - continue; // move to next block - } else if (start >= bpEnd) { // block starts after the visible context - break; // done examining blocks - } +// if (end <= bpStart) { // block ends before the visible context +// continue; // move to next block +// } else if (start >= bpEnd) { // block starts after the visible context +// break; // done examining blocks +// } boolean isSoftClip = block.isSoftClip(); @@ -834,18 +842,18 @@ private void drawAlignment( if (renderOptions.getShadeBasesOption()) { byte qual = block.getQuality(loc - start); - color = getShadedColor(qual, color, alignmentColor, prefs); + color = BaseRenderer.getShadedColor(color, qual, renderOptions.getBaseQualityMin(), renderOptions.getBaseQualityMax()); } BisulfiteBaseInfo.DisplayStatus bisstatus = (bisinfo == null) ? null : bisinfo.getDisplayStatus(idx); final boolean showBase = showAllBases || - isSoftClip || - bisulfiteMode || - // In "quick consensus" mode, only show mismatches at positions with a consistent alternative basepair. - (!quickConsensus || alignmentCounts.isConsensusMismatch(loc, reference[idx], chr, snpThreshold)); + isSoftClip || + bisulfiteMode || + // In "quick consensus" mode, only show mismatches at positions with a consistent alternative basepair. + (!quickConsensus || alignmentCounts.isConsensusMismatch(loc, reference[idx], chr, snpThreshold)); if (showBase) { - drawBase(gAlignment, color, c, pX, pY, dX, dY - (leaveMargin ? 2 : 0), bisulfiteMode, bisstatus); + BaseRenderer.drawBase(gAlignment, color, c, pX, pY, dX, dY - (leaveMargin ? 2 : 0), bisulfiteMode, bisstatus); } } } @@ -866,11 +874,11 @@ private void drawAlignment( // DRAW Insertions AlignmentBlock[] insertions = alignment.getInsertions(); if (insertions != null) { - InsertionMarker expandedInsertion = InsertionManager.getInstance().getSelectedInsertion(context.getReferenceFrame().getChrName()); - int expandedPosition = expandedInsertion == null ? -1 : expandedInsertion.position; + + for (AlignmentBlock aBlock : insertions) { - if (aBlock.getStart() == expandedPosition) continue; // Skip, will be drawn expanded + if (aBlock.getStart() == context.expandedInsertionPosition) continue; // Skip, will be drawn expanded int x = (int) ((aBlock.getStart() - bpStart) / locScale); int bpWidth = aBlock.getBasesLength(); @@ -895,7 +903,6 @@ private void drawAlignment( y, h, (int) pxWidthExact, - context.translateX, aBlock); } else { int pxWing = (h > 10 ? 2 : (h > 5) ? 1 : 0); @@ -905,7 +912,6 @@ private void drawAlignment( ig.fillRect(x - pxWing, y, 2 + 2 * pxWing, 2); ig.fillRect(x - pxWing, y + h - 2, 2 + 2 * pxWing, 2); - x += context.translateX; aBlock.setPixelRange(x - pxWing, x + 2 + pxWing); } } @@ -980,7 +986,7 @@ private static void drawClippedEnds(final Graphics2D g, final int[] xPoly, final g.drawLine(xRightPoint, yMiddle, xRight, yTop - 1); } //contig names - if(PreferencesManager.getPreferences().getAsBoolean(SAM_SHOW_CONNECTED_CHR_NAME)) { + if (PreferencesManager.getPreferences().getAsBoolean(SAM_SHOW_CONNECTED_CHR_NAME)) { if (leftContigLabel != null || rightContigLabel != null) { final int height = yBottom - yTop; int textHeight = g.getFontMetrics().getHeight(); @@ -1009,71 +1015,8 @@ private static void drawClippedEnds(final Graphics2D g, final int[] xPoly, final } } - /** - * Draw the base using either a letter or character, using the given color, - * depending on size and bisulfite status - * - * @param g - * @param color - * @param c - * @param pX - * @param pY - * @param dX - * @param dY - * @param bisulfiteMode - * @param bisstatus - */ - private void drawBase(Graphics2D g, Color color, char c, int pX, int pY, int dX, int dY, boolean bisulfiteMode, - DisplayStatus bisstatus) { - - int fontSize = Math.min(Math.min(dX, dY), 12); - if (fontSize >= 8 && (!bisulfiteMode || (bisulfiteMode && bisstatus.equals(DisplayStatus.CHARACTER)))) { - Font f = FontManager.getFont(Font.BOLD, fontSize); - g.setFont(f); - g.setColor(color); - GraphicUtils.drawCenteredText(new char[]{c}, pX, pY, dX, dY, g); - } else { - - int pX0i = pX, dXi = dX; - - // If bisulfite mode, we expand the rectangle to make it more visible - if (bisulfiteMode && bisstatus.equals(DisplayStatus.RECTANGLE)) { - if (dXi < 3) { - int expansion = dXi; - pX0i -= expansion; - dXi += (2 * expansion); - } - } - - if (color != null) { - g.setColor(color); - g.fillRect(pX0i, pY, dXi, dY); - } - } - } - - private Color getShadedColor(byte qual, Color foregroundColor, Color backgroundColor, IGVPreferences prefs) { - float alpha = 0; - int minQ = prefs.getAsInt(SAM_BASE_QUALITY_MIN); - if (qual < minQ) { - alpha = 0.1f; - } else { - int maxQ = prefs.getAsInt(SAM_BASE_QUALITY_MAX); - alpha = Math.max(0.1f, Math.min(1.0f, 0.1f + 0.9f * (qual - minQ) / (maxQ - minQ))); - } - // Round alpha to nearest 0.1 - alpha = ((int) (alpha * 10 + 0.5f)) / 10.0f; - - if (alpha >= 1) { - return foregroundColor; - } - Color color = ColorUtilities.getCompositeColor(backgroundColor, foregroundColor, alpha); - return color; - } - - private void drawLargeIndelLabel(Graphics2D g, boolean isInsertion, String labelText, int pxCenter, - int pxTop, int pxH, int pxWmax, int translateX, AlignmentBlock insertionBlock) { + int pxTop, int pxH, int pxWmax, AlignmentBlock insertionBlock) { final int pxPad = 2; // text padding in the label final int pxWing = (pxH > 10 ? 2 : 1); // width of the cursor "wing" @@ -1108,176 +1051,10 @@ private void drawLargeIndelLabel(Graphics2D g, boolean isInsertion, String label } // draw the text if it fits if (insertionBlock != null) { - insertionBlock.setPixelRange(pxLeft + translateX, pxRight + translateX); + insertionBlock.setPixelRange(pxLeft, pxRight); } } - private void drawInsertions(Rectangle rect, Alignment alignment, RenderContext context, AlignmentTrack.RenderOptions renderOptions, - AlignmentCounts alignmentCounts, boolean leaveMargin, IGVPreferences prefs) { - - AlignmentBlock[] insertions = alignment.getInsertions(); - double origin = context.getOrigin(); - double locScale = context.getScale(); - boolean flagLargeIndels = prefs.getAsBoolean(SAM_FLAG_LARGE_INDELS); - int largeInsertionsThreshold = prefs.getAsInt(SAM_LARGE_INDELS_THRESHOLD); - final float snpThreshold = prefs.getAsFloat(SAM_ALLELE_THRESHOLD); - - // TODO Quick consensus for insertions needs worked -- disabled for now - boolean quickConsensus = false;//renderOptions.quickConsensusMode; - - - if (insertions != null) { - - InsertionMarker expandedInsertion = InsertionManager.getInstance().getSelectedInsertion(context.getReferenceFrame().getChrName()); - int expandedPosition = expandedInsertion == null ? -1 : expandedInsertion.position; - - boolean hideSmallIndelsBP = renderOptions.isHideSmallIndels(); - int indelThresholdBP = renderOptions.getSmallIndelThreshold(); - - for (AlignmentBlock aBlock : insertions) { - - if (aBlock.getStart() == expandedPosition) continue; // Skip, will be drawn expanded - - int x = (int) ((aBlock.getStart() - origin) / locScale); - int bpWidth = aBlock.getBasesLength(); - double pxWidthExact = ((double) bpWidth) / locScale; - int h = (int) Math.max(1, rect.getHeight() - (leaveMargin ? 2 : 0)); - int y = (int) (rect.getY() + (rect.getHeight() - h) / 2) - (leaveMargin ? 1 : 0); - - // Don't draw out of clipping rect - if (x > rect.getMaxX()) { - break; - } else if (x < rect.getX()) { - continue; - } - - if ((!hideSmallIndelsBP || bpWidth >= indelThresholdBP)) { - // && (!quickConsensus || alignmentCounts.isConsensusInsertion(aBlock.getStart(), snpThreshold))) { - if (flagLargeIndels && bpWidth > largeInsertionsThreshold) { - drawLargeIndelLabel(context.getGraphics2D("INDEL_LABEL"), - true, - Globals.DECIMAL_FORMAT.format(bpWidth), - x - 1, - y, - h, - (int) pxWidthExact, - context.translateX, - aBlock); - } else { - int pxWing = (h > 10 ? 2 : (h > 5) ? 1 : 0); - Graphics2D g = context.getGraphics(); - g.setColor(purple); - g.fillRect(x, y, 2, h); - g.fillRect(x - pxWing, y, 2 + 2 * pxWing, 2); - g.fillRect(x - pxWing, y + h - 2, 2 + 2 * pxWing, 2); - - x += context.translateX; - aBlock.setPixelRange(x - pxWing, x + 2 + pxWing); - } - } - } - } - } - - - public void renderExpandedInsertion(InsertionMarker i, - List alignments, - RenderContext context, - Rectangle rect, - boolean leaveMargin) { - double origin = context.getOrigin(); - double locScale = context.getScale(); - if ((alignments != null) && (alignments.size() > 0)) { - - Graphics2D g = context.getGraphics2D("INSERTIONS"); - double dX = 1 / context.getScale(); - int fontSize = (int) Math.min(dX, 12); - if (fontSize >= 8) { - Font f = FontManager.getFont(Font.BOLD, fontSize); - g.setFont(f); - } - - for (Alignment alignment : alignments) { - if (alignment.getEnd() < i.position) continue; - if (alignment.getStart() > i.position) break; - AlignmentBlock insertion = alignment.getInsertionAt(i.position); - if (insertion != null) { - - // Compute the start and dend of the alignment in pixels - double pixelStart = (insertion.getStart() - origin) / locScale; - double pixelEnd = (insertion.getEnd() - origin) / locScale; - int x = (int) pixelStart; - - // If any any part of the feature fits in the track rectangle draw it - if (pixelEnd < rect.x || pixelStart > rect.getMaxX()) { - continue; - } - - int bpWidth = insertion.getBasesLength(); - double pxWidthExact = ((double) bpWidth) / locScale; - int h = (int) Math.max(1, rect.getHeight() - 2); - int y = (int) (rect.getY() + (rect.getHeight() - h) / 2) - 1; - - if (!insertion.hasBases()) { - g.setColor(purple); - g.fillRect(x, y, (int) pxWidthExact, h); - - } else { - drawExpandedInsertionBases(x, context, rect, insertion, leaveMargin); - } - } - } - } - } - - - private void drawExpandedInsertionBases(int pixelPosition, - RenderContext context, - Rectangle rect, - AlignmentBlock block, - boolean leaveMargin) { - Graphics2D g = context.getGraphics2D("INSERTIONS"); - ByteSubarray bases = block.getBases(); - int padding = block.getPadding(); - - double locScale = context.getScale(); - double origin = context.getOrigin(); - - // Compute bounds - int pY = (int) rect.getY(); - int dY = (int) rect.getHeight(); - int dX = (int) Math.max(1, (1.0 / locScale)); - - final int size = bases.length + padding; - for (int p = 0; p < size; p++) { - - char c = p < padding ? '-' : (char) bases.getByte(p - padding); - - Color color = SequenceRenderer.nucleotideColors.get(c); - if (color == null) { - color = Color.black; - } - - // If there is room for text draw the character, otherwise - // just draw a rectangle to represent the - int pX = (int) (pixelPosition + (p / locScale)); - - // Don't draw out of clipping rect - if (pX > rect.getMaxX()) { - break; - } else if (pX + dX < rect.getX()) { - continue; - } - drawBase(g, color, c, pX, pY, dX, dY - (leaveMargin ? 2 : 0), false, null); - } - - int leftX = pixelPosition + context.translateX; - int rightX = leftX + rect.width; - block.setPixelRange(leftX, rightX); - - } - - private Color getAlignmentColor(Alignment alignment, AlignmentTrack track) { // Set color used to draw the feature. Highlight features that intersect the diff --git a/src/main/java/org/broad/igv/sam/AlignmentTileLoader.java b/src/main/java/org/broad/igv/sam/AlignmentTileLoader.java index 3d30843d19..1547bd4bfd 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTileLoader.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTileLoader.java @@ -298,7 +298,7 @@ AlignmentTile loadTile(String chr, t.finish(); // TODO -- make this optional (on a preference) - InsertionManager.getInstance().processAlignments(chr, t.alignments, renderOptions); + InsertionManager.getInstance().processAlignments(chr, t.alignments); } catch (java.nio.BufferUnderflowException e) { diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrack.java b/src/main/java/org/broad/igv/sam/AlignmentTrack.java index b85ea3747e..0af84ef622 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrack.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrack.java @@ -182,7 +182,6 @@ enum OrientationType { private static final int DS_MARGIN_0 = 2; private static final int DOWNAMPLED_ROW_HEIGHT = 3; private static final int INSERTION_ROW_HEIGHT = 9; - private static final int DS_MARGIN_2 = 5; public enum BisulfiteContext { CG("CG", new byte[]{}, new byte[]{'G'}), @@ -277,7 +276,6 @@ public static boolean isBisulfiteColorType(ColorOption o) { private boolean removed = false; private boolean showGroupLine; - private Map> insertionIntervalsMap; private int expandedHeight = 14; private int collapsedHeight = 9; private final int maxSquishedHeight = 5; @@ -344,7 +342,6 @@ public AlignmentTrack(ResourceLocator locator, AlignmentDataManager dataManager, setExperimentType(ExperimentType.BISULFITE); } readNamePalette = new PaletteColorTable(ColorUtilities.getDefaultPalette()); - insertionIntervalsMap = Collections.synchronizedMap(new HashMap<>()); dataManager.setViewAsPairs(prefs.getAsBoolean(SAM_DISPLAY_PAIRED), renderOptions); @@ -366,13 +363,7 @@ public void receiveEvent(Object event) { if (event instanceof FrameManager.ChangeEvent) { // Trim insertionInterval map to current frames - Map> newMap = Collections.synchronizedMap(new HashMap<>()); - for (ReferenceFrame frame : ((FrameManager.ChangeEvent) event).getFrames()) { - if (insertionIntervalsMap.containsKey(frame)) { - newMap.put(frame, insertionIntervalsMap.get(frame)); - } - } - insertionIntervalsMap = newMap; + } else if (event instanceof AlignmentTrackEvent) { AlignmentTrackEvent e = (AlignmentTrackEvent) event; @@ -476,10 +467,7 @@ public int getHeight() { int nGroups = dataManager.getMaxGroupCount(); int h = Math.max(minHeight, getNLevels() * getRowHeight() + nGroups * GROUP_MARGIN + TOP_MARGIN - + DS_MARGIN_0 + DOWNAMPLED_ROW_HEIGHT + DS_MARGIN_2); - //if (insertionRect != null) { // TODO - replace with expand insertions preference - h += INSERTION_ROW_HEIGHT + DS_MARGIN_0; - //} + + DS_MARGIN_0 + DOWNAMPLED_ROW_HEIGHT); return Math.max(minimumHeight, h); } @@ -500,11 +488,10 @@ private int getNLevels() { @Override public boolean isReadyToPaint(ReferenceFrame frame) { - if (frame.getChrName().equals(Globals.CHR_ALL) || frame.getScale() > dataManager.getMinVisibleScale()) { + double extent = frame.getEnd() - frame.getOrigin(); + if (frame.getChrName().equals(Globals.CHR_ALL) || extent > getVisibilityWindow()) { return true; // Nothing to paint } else { - List insertionIntervals = getInsertionIntervals(frame); - insertionIntervals.clear(); return dataManager.isLoaded(frame); } } @@ -550,14 +537,6 @@ public void render(RenderContext context, Rectangle rect) { downsampleRect.height = DOWNAMPLED_ROW_HEIGHT; renderDownsampledIntervals(context, downsampleRect); - if (renderOptions.isShowInsertionMarkers()) { - insertionRect = new Rectangle(rect); - insertionRect.y += DOWNAMPLED_ROW_HEIGHT + DS_MARGIN_0; - insertionRect.height = INSERTION_ROW_HEIGHT; - renderInsertionIntervals(context, insertionRect); - rect.y = insertionRect.y + insertionRect.height; - } - alignmentsRect = new Rectangle(rect); alignmentsRect.y += 2; alignmentsRect.height -= (alignmentsRect.y - rect.y); @@ -679,7 +658,7 @@ private void renderAlignments(RenderContext context, Rectangle inputRect) { // Label the group, if there is room double groupHeight = rows.size() * h; - if (groupHeight > GROUP_LABEL_HEIGHT + 2) { + if (groupHeight > GROUP_LABEL_HEIGHT + 2 && !context.multiframe) { String groupName = entry.getKey(); Graphics2D g = context.getGraphics2D("LABEL"); FontMetrics fm = g.getFontMetrics(); @@ -696,77 +675,23 @@ private void renderAlignments(RenderContext context, Rectangle inputRect) { groupBorderGraphics.drawLine(inputRect.x, bottom, inputRect.width, bottom); } - private List getInsertionIntervals(ReferenceFrame frame) { - List insertionIntervals = insertionIntervalsMap.computeIfAbsent(frame, k -> new ArrayList<>()); - return insertionIntervals; - } - - private void renderInsertionIntervals(RenderContext context, Rectangle rect) { - - // Might be offscreen - if (!context.getVisibleRect().intersects(rect)) return; - - List intervals = context.getInsertionMarkers(); - if (intervals == null) return; - - InsertionMarker selected = InsertionManager.getInstance().getSelectedInsertion(context.getChr()); - - int w = (int) ((1.41 * rect.height) / 2); - - - boolean hideSmallIndels = renderOptions.isHideSmallIndels(); - int smallIndelThreshold = renderOptions.getSmallIndelThreshold(); - - List insertionIntervals = getInsertionIntervals(context.getReferenceFrame()); - insertionIntervals.clear(); - for (InsertionMarker insertionMarker : intervals) { - if (hideSmallIndels && insertionMarker.size < smallIndelThreshold) continue; - - final double scale = context.getScale(); - final double origin = context.getOrigin(); - int midpoint = (int) ((insertionMarker.position - origin) / scale); - int x0 = midpoint - w; - int x1 = midpoint + w; - - Rectangle iRect = new Rectangle(x0 + context.translateX, rect.y, 2 * w, rect.height); - - insertionIntervals.add(new InsertionInterval(iRect, insertionMarker)); - - Color c = (selected != null && selected.position == insertionMarker.position) ? new Color(200, 0, 0, 80) : AlignmentRenderer.purple; - Graphics2D g = context.getGraphic2DForColor(c); - - - g.fillPolygon(new Polygon(new int[]{x0, x1, midpoint}, - new int[]{rect.y, rect.y, rect.y + rect.height}, 3)); - } - } - + /** + * Render insertions at position of marker in expanded form, showing sequence. Much of this method is just + * a repeat of the loop for alignment rendering to compute the Y position of affected alignments. + * + * @param insertionMarker + * @param context + * @param inputRect + */ public void renderExpandedInsertion(InsertionMarker insertionMarker, RenderContext context, Rectangle inputRect) { - boolean leaveMargin = getDisplayMode() != DisplayMode.SQUISHED; + inputRect.y += DS_MARGIN_0 + DOWNAMPLED_ROW_HEIGHT + DS_MARGIN_0; - // Insertion interval - if (this.renderOptions.isShowInsertionMarkers()) { - Graphics2D g = context.getGraphic2DForColor(Color.red); - Rectangle iRect = new Rectangle(inputRect.x, insertionRect.y, inputRect.width, insertionRect.height); - g.fill(iRect); - - List insertionIntervals = getInsertionIntervals(context.getReferenceFrame()); - - iRect.x += context.translateX; - insertionIntervals.add(new InsertionInterval(iRect, insertionMarker)); - } - - - inputRect.y += DS_MARGIN_0 + DOWNAMPLED_ROW_HEIGHT + DS_MARGIN_0 + INSERTION_ROW_HEIGHT + DS_MARGIN_2; - - //log.debug("Render features"); final AlignmentInterval loadedInterval = dataManager.getLoadedInterval(context.getReferenceFrame(), true); PackedAlignments groups = dataManager.getGroups(loadedInterval, renderOptions); if (groups == null) { //Assume we are still loading. - //This might not always be true return; } @@ -790,10 +715,7 @@ public void renderExpandedInsertion(InsertionMarker insertionMarker, RenderConte h = squishedHeight; } - for (Map.Entry> entry : groups.entrySet()) { - - // Loop through the alignment rows for this group List rows = entry.getValue(); for (Row row : rows) { @@ -804,7 +726,8 @@ public void renderExpandedInsertion(InsertionMarker insertionMarker, RenderConte assert visibleRect != null; if (y + h > visibleRect.getY()) { Rectangle rowRectangle = new Rectangle(inputRect.x, (int) y, inputRect.width, (int) h); - renderer.renderExpandedInsertion(insertionMarker, row.alignments, context, rowRectangle, leaveMargin); + if (row.alignments != null) // TODO -- not sure this is needed + BaseRenderer.drawExpandedInsertions(insertionMarker, row.alignments, context, rowRectangle, leaveMargin, renderOptions); row.y = y; row.h = h; } @@ -818,14 +741,6 @@ public void renderExpandedInsertion(InsertionMarker insertionMarker, RenderConte } - private InsertionInterval getInsertionInterval(ReferenceFrame frame, int x, int y) { - List insertionIntervals = getInsertionIntervals(frame); - for (InsertionInterval i : insertionIntervals) { - if (i.rect.contains(x, y)) return i; - } - - return null; - } public void sortRows(final SortOption option, final Double location, final String tag, final boolean invertSort, final Set priorityRecords) { final List frames = FrameManager.getFrames(); @@ -920,17 +835,10 @@ public String getValueStringAt(String chr, double position, int mouseX, int mous return null; } } else { - - InsertionInterval insertionInterval = getInsertionInterval(frame, mouseX, mouseY); - if (insertionInterval != null) { - return "Insertions (" + insertionInterval.insertionMarker.size + " bases)"; - } else { - Alignment feature = getAlignmentAt(position, mouseY, frame); - if (feature != null) { - return feature.getAlignmentValueString(position, mouseX, renderOptions); - } + Alignment feature = getAlignmentAt(position, mouseY, frame); + if (feature != null) { + return feature.getAlignmentValueString(position, mouseX, renderOptions); } - } return null; } @@ -972,6 +880,7 @@ Alignment getAlignmentAt(double position, int y, ReferenceFrame frame) { public boolean handleDataClick(TrackClickEvent te) { MouseEvent e = te.getMouseEvent(); + if (Globals.IS_MAC && e.isMetaDown() || (!Globals.IS_MAC && e.isControlDown())) { // Selection Alignment alignment = this.getAlignmentAt(te); @@ -986,23 +895,6 @@ public boolean handleDataClick(TrackClickEvent te) { return true; } - InsertionInterval insertionInterval = getInsertionInterval(te.getFrame(), te.getMouseEvent().getX(), te.getMouseEvent().getY()); - if (insertionInterval != null) { - - final String chrName = te.getFrame().getChrName(); - InsertionMarker currentSelection = InsertionManager.getInstance().getSelectedInsertion(chrName); - if (currentSelection != null && currentSelection.position == insertionInterval.insertionMarker.position) { - InsertionManager.getInstance().clearSelected(); - } else { - InsertionManager.getInstance().setSelected(chrName, insertionInterval.insertionMarker.position); - } - - IGVEventBus.getInstance().post(new InsertionSelectionEvent(insertionInterval.insertionMarker)); - - return true; - } - - if (IGV.getInstance().isShowDetailsOnClick()) { openTooltipWindow(te); return true; @@ -1195,17 +1087,6 @@ void sendSplitToCircularView(TrackClickEvent e) { } } - private static class InsertionInterval { - - final Rectangle rect; - final InsertionMarker insertionMarker; - - InsertionInterval(Rectangle rect, InsertionMarker insertionMarker) { - this.rect = rect; - this.insertionMarker = insertionMarker; - } - } - AlignmentBlock getInsertion(Alignment alignment, int pixelX) { if (alignment != null && alignment.getInsertions() != null) { @@ -1326,31 +1207,40 @@ public static class RenderOptions implements Cloneable, Persistable { private Range groupByPos; private Boolean invertSorting; private boolean invertGroupSorting; - private Boolean showInsertionMarkers; private Boolean hideSmallIndels; private Integer smallIndelThreshold; - private BaseModficationFilter basemodFilter; - private Float basemodThreshold; + private int baseQualityMin; + + private int baseQualityMax; + BisulfiteContext bisulfiteContext = BisulfiteContext.CG; Map peStats; - public RenderOptions() { - } - RenderOptions(AlignmentTrack track) { - //updateColorScale(); this.track = track; peStats = new HashMap<>(); + + // Set some constants -- for efficiency + this.baseQualityMin = track == null ? 5 : track.getPreferences().getAsInt(SAM_BASE_QUALITY_MIN); + this.baseQualityMax = track == null ? 20 : track.getPreferences().getAsInt(SAM_BASE_QUALITY_MAX); } IGVPreferences getPreferences() { return this.track != null ? this.track.getPreferences() : AlignmentTrack.getPreferences(ExperimentType.OTHER); } + public int getBaseQualityMin() { + return baseQualityMin; + } + + public int getBaseQualityMax() { + return baseQualityMax; + } + void setShowAllBases(boolean showAllBases) { this.showAllBases = showAllBases; } @@ -1441,10 +1331,6 @@ void setLinkedReads(boolean linkedReads) { this.linkedReads = linkedReads; } - public void setShowInsertionMarkers(boolean drawInsertionIntervals) { - this.showInsertionMarkers = drawInsertionIntervals; - } - public void setHideSmallIndels(boolean hideSmallIndels) { this.hideSmallIndels = hideSmallIndels; } @@ -1482,10 +1368,6 @@ public boolean isShadeCenters() { return shadeCenters == null ? getPreferences().getAsBoolean(SAM_SHADE_CENTER) : shadeCenters; } - boolean isShowInsertionMarkers() { - return showInsertionMarkers == null ? getPreferences().getAsBoolean(SAM_SHOW_INSERTION_MARKERS) : showInsertionMarkers; - } - public boolean isFlagZeroQualityAlignments() { return flagZeroQualityAlignments == null ? getPreferences().getAsBoolean(SAM_FLAG_ZERO_QUALITY) : flagZeroQualityAlignments; } @@ -1715,13 +1597,10 @@ public void marshalXML(Document document, Element element) { if (smallIndelThreshold != null) { element.setAttribute("smallIndelThreshold", smallIndelThreshold.toString()); } - if (showInsertionMarkers != null) { - element.setAttribute("showInsertionMarkers", showInsertionMarkers.toString()); - } if (basemodFilter != null) { element.setAttribute("basemodFilter", basemodFilter.toString()); } - if(basemodThreshold != null) { + if (basemodThreshold != null) { element.setAttribute("basemodThredhold", String.valueOf(basemodThreshold)); } @@ -1755,16 +1634,16 @@ public void unmarshalXML(Element element, Integer version) { if (element.hasAttribute("colorOption")) { // Convert deprecated options final String attributeValue = element.getAttribute("colorOption"); - if("BASE_MODIFICATION_6MA".equals(attributeValue)) { + if ("BASE_MODIFICATION_6MA".equals(attributeValue)) { colorOption = ColorOption.BASE_MODIFICATION; basemodFilter = new BaseModficationFilter("a"); - } else if("BASE_MODIFICATION_5MC".equals(attributeValue)) { + } else if ("BASE_MODIFICATION_5MC".equals(attributeValue)) { colorOption = ColorOption.BASE_MODIFICATION_2COLOR; - // basemodFilter = new BaseModficationFilter(null, 'C'); - } else if("BASE_MODIFICATION_C".equals(attributeValue)) { + // basemodFilter = new BaseModficationFilter(null, 'C'); + } else if ("BASE_MODIFICATION_C".equals(attributeValue)) { colorOption = ColorOption.BASE_MODIFICATION; - // basemodFilter = new BaseModficationFilter(null, 'C'); - }else { + // basemodFilter = new BaseModficationFilter(null, 'C'); + } else { colorOption = ColorOption.valueOf(attributeValue); } } @@ -1838,7 +1717,8 @@ public void unmarshalXML(Element element, Integer version) { smallIndelThreshold = Integer.parseInt(element.getAttribute("smallIndelThreshold")); } if (element.hasAttribute("showInsertionMarkers")) { - showInsertionMarkers = Boolean.parseBoolean(element.getAttribute("showInsertionMarkers")); + // TODO -- something with this + // showInsertionMarkers = Boolean.parseBoolean(element.getAttribute("showInsertionMarkers")); } if (element.hasAttribute("basemodFilter")) { basemodFilter = BaseModficationFilter.fromString(element.getAttribute("basemodFilter")); diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index cb3b45132e..25fa117d86 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -1184,14 +1184,6 @@ void addThirdGenItems(Alignment clickedAlignment, final TrackClickEvent tce) { alignmentTrack.repaint(); })); - final JMenuItem imItem = new JCheckBoxMenuItem("Show insertion markers"); - imItem.setSelected(renderOptions.isShowInsertionMarkers()); - imItem.addActionListener(aEvt -> { - renderOptions.setShowInsertionMarkers(imItem.isSelected()); - alignmentTrack.repaint(); - }); - - add(imItem); add(qcItem); add(item); add(thresholdItem); diff --git a/src/main/java/org/broad/igv/sam/BaseRenderer.java b/src/main/java/org/broad/igv/sam/BaseRenderer.java new file mode 100644 index 0000000000..84466c6579 --- /dev/null +++ b/src/main/java/org/broad/igv/sam/BaseRenderer.java @@ -0,0 +1,156 @@ +package org.broad.igv.sam; + +import org.broad.igv.renderer.GraphicUtils; +import org.broad.igv.renderer.SequenceRenderer; +import org.broad.igv.track.RenderContext; +import org.broad.igv.ui.FontManager; +import org.broad.igv.ui.color.ColorUtilities; + +import java.awt.*; +import java.util.Collections; +import java.util.HashMap; +import java.util.List; +import java.util.Map; + +/** + * Some static methods for rendering bases in the context of alignments. * + */ + +public class BaseRenderer { + + /** + * Draw the base using either a letter or character, using the given color, + * depending on size and bisulfite status + * + * @param g + * @param color + * @param c + * @param pX + * @param pY + * @param dX + * @param dY + * @param bisulfiteMode + * @param bisstatus + */ + static void drawBase(Graphics2D g, Color color, char c, int pX, int pY, int dX, int dY, boolean bisulfiteMode, + BisulfiteBaseInfo.DisplayStatus bisstatus) { + + int fontSize = Math.min(Math.min(dX, dY), 12); + if (fontSize >= 8 && (!bisulfiteMode || (bisulfiteMode && bisstatus.equals(BisulfiteBaseInfo.DisplayStatus.CHARACTER)))) { + Font f = FontManager.getFont(Font.BOLD, fontSize); + g.setFont(f); + g.setColor(color); + GraphicUtils.drawCenteredText(new char[]{c}, pX, pY, dX, dY, g); + } else { + + // If bisulfite mode, we expand the rectangle to make it more visible + if (bisulfiteMode && bisstatus.equals(BisulfiteBaseInfo.DisplayStatus.RECTANGLE)) { + if (dX < 3) { + int expansion = dX; + pX -= expansion; + dX += (2 * expansion); + } + } + + if (color != null) { + g.setColor(color); + g.fillRect(pX, pY, dX, dY); + } + } + } + + public static void drawExpandedInsertions(InsertionMarker insertionMarker, + List alignments, + RenderContext context, + Rectangle rect, + boolean leaveMargin, + AlignmentTrack.RenderOptions renderOptions) { + + Graphics2D g = null; + try { + g = (Graphics2D) context.getGraphics().create(); + double dX = 1 / context.getScale(); + int fontSize = (int) Math.min(dX, 12); + if (fontSize >= 8) { + Font f = FontManager.getFont(Font.BOLD, fontSize); + g.setFont(f); + } + + for (Alignment alignment : alignments) { + + if (alignment.getEnd() < insertionMarker.position) continue; + if (alignment.getStart() > insertionMarker.position) break; + + AlignmentBlock insertion = alignment.getInsertionAt(insertionMarker.position); + if (insertion != null && insertion.hasBases()) { + + double origin = context.getOrigin(); + double locScale = context.getScale(); + + // Compute the start and end of the expanded insertion in pixels + int pixelStart = (int) ((insertion.getStart() - origin) / locScale); + int pixelEnd = (int) ((insertion.getEnd() - origin) / locScale) + 1; + + // Skip if insertion is out of clipping rectangle -- this probably shouldn't happen + if (pixelEnd < rect.x || pixelStart > rect.getMaxX()) { + continue; + } + + ByteSubarray bases = insertion.getBases(); + int padding = insertion.getPadding(); + + final int size = bases.length + padding; + for (int p = 0; p < size; p++) { + + int pX = (int) (pixelStart + (p / locScale)); + + // Don't draw out of clipping rect + if (pX > rect.getMaxX()) break; + else if (pX + dX < rect.getX()) continue; + + char c = p < padding ? '-' : (char) bases.getByte(p - padding); + Color color = SequenceRenderer.nucleotideColors.get(c); + if (color == null) { + color = Color.black; + } + if (renderOptions.getShadeBasesOption() && p >= padding) { + byte qual = p < padding ? (byte) 126 : insertion.getQuality(p - padding); + color = BaseRenderer.getShadedColor(color, qual, renderOptions.getBaseQualityMin(), renderOptions.getBaseQualityMax()); + } + + drawBase(g, color, c, pX, rect.y, (int) dX, rect.height - (leaveMargin ? 2 : 0), false, null); + } + insertion.setPixelRange(pixelStart, pixelEnd); + } + } + } finally { + if (g != null) g.dispose(); + } + + } + + public static Color getShadedColor(Color color, byte qual, int minQ, int maxQ) { + + if (qual >= maxQ) return color; + + float alpha; + if (qual < minQ) { + alpha = 0.2f; + } else { + alpha = Math.max(0.2f, Math.min(1.0f, 0.1f + 0.9f * (qual - minQ) / (maxQ - minQ))); + } + + // Round alpha to nearest 0.1 + alpha = ((int) (alpha * 10 + 0.5f)) / 10.0f; + String key = ColorUtilities.colorToString(color) + "_" + alpha; + Color c = shadedColorCache.get(key); + if (c == null) { + c = ColorUtilities.modifyAlpha(color, (int) (alpha * 255)); + shadedColorCache.put(key, c); + } + return c; + } + + static Map shadedColorCache = Collections.synchronizedMap(new HashMap<>()); + +} diff --git a/src/main/java/org/broad/igv/sam/CoverageTrack.java b/src/main/java/org/broad/igv/sam/CoverageTrack.java index 7dc9a68fb3..05d4b62c59 100644 --- a/src/main/java/org/broad/igv/sam/CoverageTrack.java +++ b/src/main/java/org/broad/igv/sam/CoverageTrack.java @@ -172,7 +172,8 @@ public void setDataSource(CoverageDataSource dataSource) { @Override public boolean isReadyToPaint(ReferenceFrame frame) { - if (frame.getChrName().equals(Globals.CHR_ALL) || frame.getScale() > dataManager.getMinVisibleScale()) { + double extent = frame.getEnd() - frame.getOrigin(); + if (frame.getChrName().equals(Globals.CHR_ALL) || extent > dataManager.getVisibilityWindow()) { return true; // Nothing to paint } else { return dataManager.isLoaded(frame); diff --git a/src/main/java/org/broad/igv/sam/InsertionManager.java b/src/main/java/org/broad/igv/sam/InsertionManager.java index 3ddf33af65..be96920350 100644 --- a/src/main/java/org/broad/igv/sam/InsertionManager.java +++ b/src/main/java/org/broad/igv/sam/InsertionManager.java @@ -28,9 +28,12 @@ import org.broad.igv.feature.genome.Genome; import org.broad.igv.feature.genome.GenomeManager; +import org.broad.igv.prefs.Constants; +import org.broad.igv.prefs.IGVPreferences; import org.broad.igv.prefs.PreferencesManager; import java.util.*; +import java.util.stream.Collectors; import static org.broad.igv.prefs.Constants.SAM_HIDE_SMALL_INDEL; import static org.broad.igv.prefs.Constants.SAM_SMALL_INDEL_BP_THRESHOLD; @@ -45,61 +48,45 @@ public class InsertionManager { private static InsertionManager theInstance = new InsertionManager(); private Map> insertionMaps; - private Map> positionsMap; private Map selectedInsertions; + InsertionMarker selectedInsertion; + public static InsertionManager getInstance() { return theInstance; } private InsertionManager() { this.insertionMaps = Collections.synchronizedMap(new HashMap<>(100)); - this.positionsMap = Collections.synchronizedMap(new HashMap<>(100)); this.selectedInsertions = Collections.synchronizedMap(new HashMap<>(100)); } public void clear() { this.insertionMaps.clear(); - this.positionsMap.clear(); this.selectedInsertions.clear();; + this.selectedInsertion = null; } public List getInsertions(String chrName, double start, double end) { Map insertionMap = insertionMaps.get(chrName); - List positions = positionsMap.get(chrName); - if(insertionMap == null || positions == null) return null; - - List insertionMarkers = new ArrayList<>(); - for (int i = 0; i < positions.size(); i++) { - final Integer key = positions.get(i); - if (key > end) break; - if (key >= start) { - final InsertionMarker insertionMarker = insertionMap.get(key); - insertionMarkers.add(insertionMarker); - } - } - return insertionMarkers; + if(insertionMap == null ) return null; - } + return insertionMap.values().stream().filter(im -> im.position + im.size >= start && im.position <= end).collect(Collectors.toList()); - public void setSelected(String chrName, int position) { - this.selectedInsertions.put(chrName, position); } - public void clearSelected() { - this.selectedInsertions.clear(); + public void setSelected(InsertionMarker insertionMarker) { + this.selectedInsertion = insertionMarker; } public InsertionMarker getSelectedInsertion(String chrName) { - Integer selectedInsertion = selectedInsertions.get(chrName); - Map insertionMap = insertionMaps.get(chrName); - return (selectedInsertion == null || insertionMap == null) ? null : insertionMap.get(selectedInsertion); + return this.selectedInsertion; } - - public synchronized void processAlignments(String chr, List alignments, AlignmentTrack.RenderOptions renderOptions) { + + public synchronized void processAlignments(String chr, List alignments) { Genome genome = GenomeManager.getInstance().getCurrentGenome(); chr = genome == null ? chr : genome.getCanonicalChrName(chr); @@ -109,15 +96,11 @@ public synchronized void processAlignments(String chr, List alignment insertionMap = Collections.synchronizedMap(new HashMap<>()); insertionMaps.put(chr, insertionMap); } - List positions = positionsMap.get(chr); - if(positions == null) { - positions = new ArrayList<>(); - positionsMap.put(chr, positions); - } int minLength = 0; - if (renderOptions != null && renderOptions.isHideSmallIndels()) { - minLength = renderOptions.getSmallIndelThreshold(); + final IGVPreferences preferences = PreferencesManager.getPreferences(Constants.THIRD_GEN); + if(preferences.getAsBoolean(SAM_HIDE_SMALL_INDEL)) { + minLength = preferences.getAsInt(SAM_SMALL_INDEL_BP_THRESHOLD); } for (Alignment a : alignments) { @@ -130,17 +113,12 @@ public synchronized void processAlignments(String chr, List alignment if (insertionMarker == null) { insertionMarker = new InsertionMarker(block.getStart(), block.getLength()); insertionMap.put(key, insertionMarker); - positions.add(block.getStart()); } else { insertionMarker.size = Math.max(insertionMarker.size, block.getLength()); } } } } - - positions.addAll(insertionMap.keySet()); - positions.sort((o1, o2) -> o1 - o2); } - } diff --git a/src/main/java/org/broad/igv/sam/InsertionMarker.java b/src/main/java/org/broad/igv/sam/InsertionMarker.java index 63a752a598..7eae8972cd 100644 --- a/src/main/java/org/broad/igv/sam/InsertionMarker.java +++ b/src/main/java/org/broad/igv/sam/InsertionMarker.java @@ -1,15 +1,30 @@ package org.broad.igv.sam; +import java.util.Objects; + /** * Created by jrobinso on 1/13/17. */ public class InsertionMarker { + public int position; public int size; - public int pixelPosition = -1; public InsertionMarker(int position, int size) { this.position = position; this.size = size; } + + @Override + public boolean equals(Object o) { + if (this == o) return true; + if (o == null || getClass() != o.getClass()) return false; + InsertionMarker that = (InsertionMarker) o; + return position == that.position && size == that.size; + } + + @Override + public int hashCode() { + return Objects.hash(position, size); + } } diff --git a/src/main/java/org/broad/igv/sam/SpliceJunctionTrack.java b/src/main/java/org/broad/igv/sam/SpliceJunctionTrack.java index d3078f25fd..be6ddec058 100644 --- a/src/main/java/org/broad/igv/sam/SpliceJunctionTrack.java +++ b/src/main/java/org/broad/igv/sam/SpliceJunctionTrack.java @@ -236,7 +236,8 @@ public void load(ReferenceFrame frame) { @Override public boolean isReadyToPaint(ReferenceFrame frame) { - if (frame.getChrName().equals(Globals.CHR_ALL) || frame.getScale() > dataManager.getMinVisibleScale()) { + double extent = frame.getEnd() - frame.getOrigin(); + if (frame.getChrName().equals(Globals.CHR_ALL) || extent > dataManager.getVisibilityWindow()) { return true; // Nothing to paint } else { diff --git a/src/main/java/org/broad/igv/session/Session.java b/src/main/java/org/broad/igv/session/Session.java index 9c6d6dfce3..43706b50ec 100644 --- a/src/main/java/org/broad/igv/session/Session.java +++ b/src/main/java/org/broad/igv/session/Session.java @@ -97,11 +97,10 @@ public enum GeneListMode { private String locus; public Session(String path) { - reset(path); + init(path); } - public void reset(String path) { - + private void init(String path) { this.path = path; this.nextAutoscaleGroup = 1; this.groupByAttribute = null; @@ -111,15 +110,20 @@ public void reset(String path) { this.colorScales = new HashMap<>(); this.hiddenAttributes = null; this.history = new History(100); + IGVEventBus.getInstance().subscribe(ViewChange.class, this); + } - boolean resetRequired = FrameManager.getFrames().size() > 1; + public void reset(String path) { + init(path); setCurrentGeneList(null); - if (resetRequired) { + if (FrameManager.getFrames().size() > 1) { IGV.getInstance().resetFrames(); } - + for(ReferenceFrame frame : FrameManager.getFrames()) { + frame.setExpandedInsertion(null); + } InsertionManager.getInstance().clear(); - IGVEventBus.getInstance().subscribe(ViewChange.class, this); + } diff --git a/src/main/java/org/broad/igv/track/RenderContext.java b/src/main/java/org/broad/igv/track/RenderContext.java index e5e9c5e007..0b9712bd17 100644 --- a/src/main/java/org/broad/igv/track/RenderContext.java +++ b/src/main/java/org/broad/igv/track/RenderContext.java @@ -51,12 +51,9 @@ public class RenderContext { private Map graphicCache; private ReferenceFrame referenceFrame; private JComponent panel; - private Rectangle visibleRect; - private boolean merged = false; - public transient int translateX; - private List insertionMarkers; + public Rectangle visibleRect; public boolean multiframe = false; - + public int expandedInsertionPosition = -1; public RenderContext(JComponent panel, Graphics2D graphics, ReferenceFrame referenceFrame, Rectangle visibleRect) { this.graphics = graphics; @@ -70,11 +67,12 @@ public RenderContext(JComponent panel, Graphics2D graphics, ReferenceFrame refer } public RenderContext(RenderContext context) { - this.graphics = context.graphics; + this.graphics = (Graphics2D) context.graphics.create(); this.graphicCache = new HashMap<>(); this.referenceFrame = new ReferenceFrame(context.referenceFrame); this.panel = context.panel; this.visibleRect = new Rectangle(context.visibleRect); + this.expandedInsertionPosition = context.expandedInsertionPosition; if (PreferencesManager.getPreferences().getAntiAliasing() && graphics != null) { graphics.setRenderingHint(RenderingHints.KEY_TEXT_ANTIALIASING, RenderingHints.VALUE_TEXT_ANTIALIAS_ON); } @@ -153,12 +151,4 @@ public void dispose() { graphicCache.clear(); } - - public void setInsertionMarkers(List insertionMarkers) { - this.insertionMarkers = insertionMarkers; - } - - public List getInsertionMarkers() { - return insertionMarkers; - } } diff --git a/src/main/java/org/broad/igv/ui/panel/DataPanel.java b/src/main/java/org/broad/igv/ui/panel/DataPanel.java index 51e77e72d8..bce45fc33b 100644 --- a/src/main/java/org/broad/igv/ui/panel/DataPanel.java +++ b/src/main/java/org/broad/igv/ui/panel/DataPanel.java @@ -151,7 +151,7 @@ public void paintComponent(final Graphics g) { computeMousableRegions(groups, trackWidth); - painter.paint(groups, context, trackWidth, getBackground(), damageRect); + painter.paint(groups, context, getBackground(), damageRect); // If there is a partial ROI in progress draw it first if (currentTool instanceof RegionOfInterestTool) { @@ -251,7 +251,7 @@ public void paintOffscreen(final Graphics2D g, Rectangle rect, boolean batch) { rect.width - (insets.left + insets.right), rect.height - (insets.top + insets.bottom)); context.getGraphics().setClip(contentRect); - painter.paint(groups, context, contentRect.width, getBackground(), contentRect); + painter.paint(groups, context, getBackground(), contentRect); drawAllRegions(g); borderGraphics.drawRect(0, rect.y, rect.width, rect.height-1); diff --git a/src/main/java/org/broad/igv/ui/panel/DataPanelPainter.java b/src/main/java/org/broad/igv/ui/panel/DataPanelPainter.java index 90085b2e4e..93a96cdc0d 100644 --- a/src/main/java/org/broad/igv/ui/panel/DataPanelPainter.java +++ b/src/main/java/org/broad/igv/ui/panel/DataPanelPainter.java @@ -52,76 +52,88 @@ public class DataPanelPainter { public synchronized void paint(Collection groups, RenderContext context, - int width, Color background, Rectangle visibleRect) { - - // - Graphics2D graphics2D = context.getGraphics2D("BACKGROUND"); graphics2D.setBackground(background); graphics2D.clearRect(visibleRect.x, visibleRect.y, visibleRect.width, visibleRect.height); graphics2D.setColor(Color.BLACK); - final ReferenceFrame referenceFrame = context.getReferenceFrame(); - context.setInsertionMarkers(InsertionManager.getInstance().getInsertions(referenceFrame.getChrName(), referenceFrame.getOrigin(), referenceFrame.getEnd())); + InsertionMarker i = referenceFrame.getExpandedInsertion(); //insertionManager.getSelectedInsertion(referenceFrame.getChrName()); - InsertionMarker i = InsertionManager.getInstance().getSelectedInsertion(referenceFrame.getChrName()); + if (i != null) { + Collection tracks = IGV.getInstance().getAlignmentTracks(); + int maxVizWindow = tracks.size() == 0 ? 0 : tracks.stream().mapToInt(t -> t.getVisibilityWindow()).max().getAsInt(); - if (i != null) { final double start = referenceFrame.getOrigin(); - try { - double scale = referenceFrame.getScale(); - int p0 = 0, w; + final double frameExtent = referenceFrame.getEnd() - referenceFrame.getOrigin(); + double scale = referenceFrame.getScale(); + int insertionPixelPosition = (int) ((i.position - start) / scale); + int insertionPixelWidth = (int) Math.ceil(i.size / context.getScale()); + - w = (int) ((i.position - start) / scale); - paintSection(groups, context, p0, visibleRect.y, w, visibleRect.height, 0); + if (frameExtent < maxVizWindow && + insertionPixelWidth > 1 && + insertionPixelPosition < visibleRect.width && + insertionPixelPosition + insertionPixelWidth > 0) { - i.pixelPosition = p0 + w; - p0 += w; + context.expandedInsertionPosition = i.position; - context.multiframe = true; - referenceFrame.origin = i.position; - w = (int) Math.ceil(i.size / context.getScale()); - paintExpandedInsertion(i, groups, context, p0, visibleRect.y, w, visibleRect.height); + // Paint section left of insertion + if (insertionPixelPosition > 0) { + int width = insertionPixelPosition; + RenderContext leftContext = shiftRenderContext(context, context.getOrigin(), 0, width); + paintFrame(groups, leftContext); + } + + // Paint expanded insertion + RenderContext insertionContext = shiftRenderContext(context, i.position, insertionPixelPosition, insertionPixelWidth); + paintExpandedInsertion(i, groups, insertionContext); - int g = (int) (i.size / scale); - p0 += g; - context.getReferenceFrame().origin = i.position; - w = width - p0; + // Paint section to right of insertion + int p0 = insertionPixelPosition + insertionPixelWidth; + int w = visibleRect.width - p0; if (w > 0) { - paintSection(groups, context, p0, visibleRect.y, width - p0, visibleRect.height, g); + RenderContext rightContext = shiftRenderContext(context, i.position, p0, w); + rightContext.multiframe = true; + paintFrame(groups, rightContext); } - } finally { - referenceFrame.origin = start; + + } else { + // Insertion is out of view + paintFrame(groups, context); } } else { - paintFrame(groups, context, width, visibleRect); + // No expanded insertion + paintFrame(groups, context); } } - private void paintSection(Collection groups, RenderContext context, int px, int py, int w, int h, int delta) { + private RenderContext shiftRenderContext(RenderContext ctx, double position, int translateX, int pixelWidth) { + RenderContext newContext = new RenderContext(ctx); + newContext.getReferenceFrame().widthInPixels = pixelWidth; + newContext.getReferenceFrame().origin = position; + newContext.visibleRect = new Rectangle(0, ctx.visibleRect.y, pixelWidth, ctx.visibleRect.height); - context.clearGraphicsCache(); - Graphics2D dG = context.getGraphics(); - Rectangle dRect = new Rectangle(0, py, w, h); - dG.translate(delta, 0); - dG.setClip(dRect); - context.translateX = px; - - paintFrame(groups, context, w, dRect); + Graphics2D dG = newContext.getGraphics(); + Rectangle dRect = newContext.visibleRect; + dG.translate(translateX, 0); + dG.setClip(newContext.visibleRect); + return newContext; } - private void paintFrame(Collection groups, RenderContext dContext, int width, Rectangle dRect) { + private void paintFrame(Collection groups, RenderContext dContext) { + int trackX = 0; int trackY = 0; + Rectangle dRect = dContext.visibleRect; for (Iterator groupIter = groups.iterator(); groupIter.hasNext(); ) { TrackGroup group = groupIter.next(); @@ -133,14 +145,14 @@ private void paintFrame(Collection groups, RenderContext dContext, i if (group.isVisible()) { if (groups.size() > 1) { final Graphics2D greyGraphics = dContext.getGraphic2DForColor(UIConstants.LIGHT_GREY); - greyGraphics.fillRect(0, trackY + 1, width, UIConstants.groupGap - 1); + greyGraphics.fillRect(0, trackY + 1, dRect.width, UIConstants.groupGap - 1); trackY += UIConstants.groupGap; } // Draw a line just above group. if (group.isDrawBorder()) { Graphics2D graphics2D = dContext.getGraphic2DForColor(Color.black); - graphics2D.drawLine(0, trackY - 1, width, trackY - 1); + graphics2D.drawLine(0, trackY - 1, dRect.width, trackY - 1); } List trackList = group.getVisibleTracks(); @@ -161,7 +173,7 @@ private void paintFrame(Collection groups, RenderContext dContext, i if (track.isVisible()) { - Rectangle rect = new Rectangle(trackX, trackY, width, trackHeight); + Rectangle rect = new Rectangle(trackX, trackY, dRect.width, trackHeight); draw(track, rect, dContext); trackY += trackHeight; } @@ -171,23 +183,18 @@ private void paintFrame(Collection groups, RenderContext dContext, i // Draw a line just below group. if (group.isDrawBorder()) { Graphics2D graphics2D = dContext.getGraphic2DForColor(Color.black); - graphics2D.drawLine(0, trackY, width, trackY); + graphics2D.drawLine(0, trackY, dRect.width, trackY); } } } } - private void paintExpandedInsertion(InsertionMarker insertionMarker, Collection groups, RenderContext context, int px, int py, int w, int h) { + private void paintExpandedInsertion(InsertionMarker insertionMarker, Collection groups, RenderContext context) { - context.clearGraphicsCache(); - Graphics2D dG = context.getGraphics(); - Rectangle dRect = new Rectangle(0, py, w, h); - dG.translate(px, 0); - dG.setClip(dRect); - context.translateX = px; int trackY = 0; + Rectangle dRect = context.getVisibleRect(); for (Iterator groupIter = groups.iterator(); groupIter.hasNext(); ) { TrackGroup group = groupIter.next(); @@ -229,7 +236,6 @@ final private void draw(Track track, Rectangle rect, RenderContext context) { track.render(context, rect); // Get overlays - List overlayTracks = IGV.getInstance().getOverlayTracks(track); if (overlayTracks != null) { for (Track overlayTrack : overlayTracks) { diff --git a/src/main/java/org/broad/igv/ui/panel/ReferenceFrame.java b/src/main/java/org/broad/igv/ui/panel/ReferenceFrame.java index 2758372c7a..25f3c36f16 100644 --- a/src/main/java/org/broad/igv/ui/panel/ReferenceFrame.java +++ b/src/main/java/org/broad/igv/ui/panel/ReferenceFrame.java @@ -122,6 +122,7 @@ public class ReferenceFrame { protected volatile double scale; protected Locus initialLocus = null; + private InsertionMarker expandedInsertion; public ReferenceFrame(String name) { @@ -380,7 +381,7 @@ public void recordHistory() { public void shiftOriginPixels(int delta) { double shiftBP = delta * getScale(); - setOrigin(origin + shiftBP); + setOrigin(shiftBP + origin); eventBus.post(ViewChange.Result()); } @@ -454,12 +455,24 @@ public double getOrigin() { return origin; } + /** + * Return the origin in reference genome coordinates. When an insertion is expanded "origin" is in expansion modified + * coordinates, we need to adjust for the expansion to convert to reference genome coordinates* * + * @return + */ + public double getExpansionAdjustedOrigin() { + InsertionMarker im = getExpandedInsertion(); + return (im == null || origin < im.position) ? + origin : + origin - im.size; + } + public double getCenter() { return origin + getScale() * widthInPixels / 2; } public double getEnd() { - return origin + getScale() * widthInPixels; + return getChromosomePosition(widthInPixels); } public int getZoom() { @@ -517,59 +530,53 @@ public double getChromosomePosition(final MouseEvent e) { } /** - * Return the chromosome position corresponding to the pixel index. The - * pixel index is the pixel "position" translated by -origin. + * Return the chromosome position corresponding to the screen (pixel) position. * * @param screenPosition * @return */ public double getChromosomePosition(int screenPosition) { - - InsertionMarker i = InsertionManager.getInstance().getSelectedInsertion(getChrName()); - - if (i != null && i.position > origin) { - // if (IGV.getInstance().getSession().expandInsertions && insertionMarkers != null && insertionMarkers.size() > 0) { - double start = getOrigin(); - double scale = getScale(); - double iEnd = 0, - iStart = 0; - - - iStart = iEnd + (i.position - start) / scale; // Screen position of insertionMarker start - if (screenPosition < iStart) { - return start + scale * (screenPosition - iEnd); + InsertionMarker i = expandedInsertion; + if (i != null) { + int insertionPixelPosition = (int) ((i.position - origin) / scale); + int insertionPixelWidth = (int) Math.ceil(i.size / scale); + if (insertionPixelPosition < 0) { + // Only a portion of the insertion is occupying screen + insertionPixelWidth += insertionPixelPosition; + insertionPixelPosition = 0; } - - iEnd = iStart + i.size / scale; // Screen position of insertionMarker end - if (screenPosition < iEnd) { - return i.position; // In the gap + if (insertionPixelWidth > 0) { + if (screenPosition < insertionPixelPosition) { + // Nothing to do -- expanded insertion is to the right of screen position + } else if (screenPosition < insertionPixelPosition + insertionPixelWidth) { + // Over expanded insertion, chromosome position is position of insertion + screenPosition = insertionPixelPosition; + } else { + // To the right of the expanded insertion + screenPosition -= insertionPixelWidth; + } } - - start = i.position + 1; - // } - return start + scale * (screenPosition - iEnd); - - } else { - return origin + getScale() * screenPosition; } - + return origin + getScale() * screenPosition; } /** - * Return the screen position corresponding to the chromosomal position. + * Return the screen position corresponding to the chromosomal position, accounting for the possibility of + * an expanded insertion in view. For the special case when chromosomePosition (i.e. genomic coordinate) + * is at the expanded insertion there is no unique screen position. By convention we return the screen position + * at the left boundary of the expansion * - * @param chromosomePosition - * @return + * @param chromosomePosition The genomic position in base pairs + * @return screen position (pixels) */ public int getScreenPosition(double chromosomePosition) { - InsertionMarker i = InsertionManager.getInstance().getSelectedInsertion(chrName); - - if (i == null || i.position < origin || i.position > chromosomePosition) { - return (int) ((chromosomePosition - origin) / getScale()); + // If there is an expanded insertion in view we need to account for the empty screen space + if (expandedInsertion != null && chromosomePosition > expandedInsertion.position) { + return (int) ((chromosomePosition + expandedInsertion.size - origin) / getScale()); } else { - return (int) ((chromosomePosition + i.size - origin) / getScale()); + return (int) ((chromosomePosition - origin) / getScale()); } } @@ -764,5 +771,12 @@ private static Genome getGenome() { } + public void setExpandedInsertion(InsertionMarker im) { + this.expandedInsertion = im; + } + + public InsertionMarker getExpandedInsertion() { + return expandedInsertion; + } } diff --git a/src/main/java/org/broad/igv/ui/panel/RegionOfInterestPanel.java b/src/main/java/org/broad/igv/ui/panel/RegionOfInterestPanel.java index b1b183e87b..30a007b8b4 100644 --- a/src/main/java/org/broad/igv/ui/panel/RegionOfInterestPanel.java +++ b/src/main/java/org/broad/igv/ui/panel/RegionOfInterestPanel.java @@ -31,6 +31,11 @@ import org.broad.igv.Globals; import org.broad.igv.feature.Strand; +import org.broad.igv.sam.AlignmentInterval; +import org.broad.igv.sam.AlignmentRenderer; +import org.broad.igv.sam.InsertionManager; +import org.broad.igv.sam.InsertionMarker; +import org.broad.igv.track.RenderContext; import org.broad.igv.util.blat.BlatClient; import org.broad.igv.feature.RegionOfInterest; import org.broad.igv.feature.genome.Genome; @@ -46,6 +51,7 @@ import java.awt.event.ActionListener; import java.awt.event.MouseEvent; import java.util.Collection; +import java.util.List; /** * @author eflakes @@ -77,6 +83,8 @@ public void paintComponent(final Graphics g) { // Draw regions of interest? drawRegionsOfInterest((Graphics2D) g, getHeight()); + //drawInsertionMarkers((Graphics2D) g, getHeight()); + g.setColor(Color.BLACK); g.drawLine(0, 0, getWidth(), 0); } @@ -90,7 +98,6 @@ public void drawRegionsOfInterest(final Graphics2D g, int height) { return; } - for (RegionOfInterest regionOfInterest : regions) { int regionStart = regionOfInterest.getStart(); @@ -113,7 +120,6 @@ public void drawRegionsOfInterest(final Graphics2D g, int height) { } } - /** * Return the region of interest at the screen pixel location. * diff --git a/src/main/java/org/broad/igv/ui/panel/RulerPanel.java b/src/main/java/org/broad/igv/ui/panel/RulerPanel.java index efae7dbc6f..fbc808be4b 100644 --- a/src/main/java/org/broad/igv/ui/panel/RulerPanel.java +++ b/src/main/java/org/broad/igv/ui/panel/RulerPanel.java @@ -40,10 +40,11 @@ import org.broad.igv.feature.genome.Genome; import org.broad.igv.feature.genome.GenomeManager; import org.broad.igv.prefs.PreferencesManager; +import org.broad.igv.sam.AlignmentTrack; import org.broad.igv.sam.InsertionManager; import org.broad.igv.sam.InsertionMarker; import org.broad.igv.ui.FontManager; -import org.broad.igv.ui.UIConstants; +import org.broad.igv.ui.IGV; import org.broad.igv.ui.WaitCursorManager; import org.broad.igv.util.LongRunningTask; import org.broad.igv.util.NamedRunnable; @@ -54,33 +55,30 @@ import java.awt.event.MouseEvent; import java.text.DecimalFormat; import java.util.ArrayList; +import java.util.Collection; import java.util.List; +import java.util.function.Consumer; -import static org.broad.igv.prefs.Constants.DEFAULT_GENOME; -import static org.broad.igv.prefs.Constants.ENABLE_ANTIALISING; +import static org.broad.igv.prefs.Constants.*; /** * @author jrobinso */ public class RulerPanel extends JPanel { + public static final Color INSERTION_MARKER_ZOOMEDOUT = new Color(64, 64, 64, 50); private static Logger log = LogManager.getLogger(RulerPanel.class); private static final DecimalFormat DECIMAL_FORMAT = new DecimalFormat(); - private static Color grey1 = new Color(120, 120, 120); - private static Color grey2 = new Color(200, 200, 200); - private static Color gene1 = new Color(0, 0, 150, 150); - private static Color gene2 = new Color(0, 150, 0, 150); + private static final int INSERTION_ROW_HEIGHT = 9; // TODO -- get from preferences boolean drawSpan = true; private Font tickFont = FontManager.getFont(Font.BOLD, 9); private Font spanFont = FontManager.getFont(Font.BOLD, 12); - private List chromosomeRects = new ArrayList(); - private List mouseRects = new ArrayList(); - + private List clickLinks = new ArrayList(); private static Color dragColor = new Color(.5f, .5f, 1f, .3f); private static Color zoomBoundColor = new Color(0.5f, 0.5f, 0.5f); @@ -131,23 +129,27 @@ protected void paintComponent(Graphics g) { } private void render(Graphics g) { - g.setColor(Color.black); + clickLinks.clear(); + g.setColor(Color.black); if (isWholeGenomeView()) { drawChromosomeTicks(g); } else { - - InsertionMarker i = InsertionManager.getInstance().getSelectedInsertion(frame.getChrName()); - - drawTicks(g, i); - + drawTicks(g); if (drawSpan) { - drawSpan(g, i); + drawSpan(g); + } + + // If zoomed in, use the 3rd gen visibility window + Collection tracks = IGV.getInstance().getAlignmentTracks(); + int maxVizWindow = tracks.size() == 0 ? 0 : tracks.stream().mapToInt(t -> t.getVisibilityWindow()).max().getAsInt(); + if (!frame.getChrName().equals(Globals.CHR_ALL) && (frame.getEnd() - frame.getOrigin()) <= maxVizWindow) { + drawInsertionMarkers(g); } } } - private void drawSpan(Graphics g, InsertionMarker i) { + private void drawSpan(Graphics g) { //TODO -- hack int w = getWidth(); @@ -180,7 +182,7 @@ private void drawSpan(Graphics g, InsertionMarker i) { } - private void drawTicks(Graphics g, InsertionMarker i) { + private void drawTicks(Graphics g) { int w = getWidth(); if (w < 200) { @@ -197,7 +199,7 @@ private void drawTicks(Graphics g, InsertionMarker i) { double spacing = ts.getMajorTick(); // Find starting point closest to the current origin - int nTick = (int) (frame.getOrigin() / spacing) - 1; + int nTick = (int) (frame.getExpansionAdjustedOrigin() / spacing) - 1; int l = (int) (nTick * spacing); int x = frame.getScreenPosition(l - 1 + 0.5); // 0 vs 1 based coordinates, then center over base //int strEnd = Integer.MIN_VALUE; @@ -213,7 +215,7 @@ private void drawTicks(Graphics g, InsertionMarker i) { if (nTick % 2 == 0 && strPosition > 0) { g.drawString(chrPosition, strPosition, height - 15); } - if(x > 0) { + if (x > 0) { g.drawLine(x, height - 10, x, height - 2); } @@ -221,13 +223,16 @@ private void drawTicks(Graphics g, InsertionMarker i) { } } + /** + * Draw whole genome view, one "tick" per chromosome. * + * @param g + */ private void drawChromosomeTicks(Graphics g) { Font chrFont = FontManager.getFont(10); - //this.removeAll(); this.setLayout(null); - // TODO -- remove hardcoded value + // Whole genome view uses kb units int locationUnit = 1000; g.setFont(chrFont); @@ -240,8 +245,6 @@ private void drawChromosomeTicks(Graphics g) { } boolean even = true; - long offset = 0; - chromosomeRects.clear(); List chrNames = genome.getLongChromosomeNames(); if (chrNames == null) { log.info("No chromosomes found for genome: " + PreferencesManager.getPreferences().getDefaultGenome()); @@ -252,7 +255,7 @@ private void drawChromosomeTicks(Graphics g) { } final FontMetrics fontMetrics = g.getFontMetrics(); - for (String chrName : chrNames) { + for (final String chrName : chrNames) { Chromosome c = genome.getChromosome(chrName); if (c == null) { log.info("Chromosome '" + chrName + "' not found"); @@ -265,7 +268,7 @@ private void drawChromosomeTicks(Graphics g) { int x = (int) (gStart / scale); int dw = (int) (chrLength / (locationUnit * scale)); - if(x > 0) { + if (x > 0) { g.drawLine(x, getHeight() - 10, x, getHeight() - 2); } @@ -285,19 +288,85 @@ private void drawChromosomeTicks(Graphics g) { int y = (even ? getHeight() - 35 : getHeight() - 25); g.drawString(displayName, strPosition, y); - int sw = (int) fontMetrics.getStringBounds(displayName, g).getWidth(); - Rectangle clickRect = new Rectangle(strPosition, y - 15, sw, 15); - String tooltipText = "Jump to chromosome: " + chrName; - chromosomeRects.add(new ClickLink(clickRect, chrName, tooltipText)); + + // Create clickable link if not dragging + if (!dragging) { + int sw = (int) fontMetrics.getStringBounds(displayName, g).getWidth(); + Rectangle clickRect = new Rectangle(strPosition, y - 15, sw, 15); + String tooltipText = "Jump to chromosome: " + chrName; + clickLinks.add(new ClickLink(clickRect, chrName, tooltipText, ch -> { + frame.changeChromosome((String) ch, true); + })); + } even = !even; } + } + } + + private void drawInsertionMarkers(Graphics g) { + + List insertionMarkers = InsertionManager.getInstance().getInsertions(frame.getChrName(), frame.getOrigin(), frame.getEnd() + 1); + + if (insertionMarkers == null) return; + + InsertionMarker expanded = frame.getExpandedInsertion(); + + int w = (int) ((1.41 * INSERTION_ROW_HEIGHT) / 2); + int y = getHeight() - INSERTION_ROW_HEIGHT - 1; + + for (InsertionMarker insertionMarker : insertionMarkers) { + + int x0; + int x1; + Polygon p; + Color c; + if (expanded != null && insertionMarker.position == expanded.position) { + x0 = frame.getScreenPosition(insertionMarker.position); + x1 = (int) ((insertionMarker.position + insertionMarker.size - frame.origin) / frame.getScale()); + p = new Polygon( + new int[]{x0 - w, x1 + w, x1, x0}, + new int[]{y, y, y + INSERTION_ROW_HEIGHT, y + INSERTION_ROW_HEIGHT}, 4); + c = Color.BLUE; + + String tooltipText = "Click to collapse insertion"; + clickLinks.add(new ClickLink(p, null, tooltipText, obj -> { + frame.setExpandedInsertion((InsertionMarker) obj); + IGV.getInstance().repaint(); + })); + + } else { + x0 = frame.getScreenPosition(insertionMarker.position); + x1 = x0; + p = new Polygon(new int[]{x0 - w, x1 + w, x0}, + new int[]{y, y, y + INSERTION_ROW_HEIGHT}, 3); + + double expandedInsertionWidth = insertionMarker.size / frame.getScale(); + + if(expandedInsertionWidth > 5) { + c = Color.BLACK ; + String tooltipText = "Click to expand insertion (" + (insertionMarker.size + "bases)"); + Rectangle clickArea = p.getBounds(); + clickArea.y -= 2; + clickArea.height += 2; + clickLinks.add(new ClickLink(clickArea, insertionMarker, tooltipText, obj -> { + frame.setExpandedInsertion((InsertionMarker) obj); + IGV.getInstance().repaint(); + })); + } else { + c = INSERTION_MARKER_ZOOMEDOUT; + } + } - offset += chrLength; + if (x1 + w >= 0 && x0 - w <= getWidth()) { + g.setColor(c); + g.fillPolygon(p); + } } } + public static String formatNumber(double position) { //NumberFormatter f = new NumberFormatter(); @@ -353,27 +422,16 @@ private void init() { @Override public void mouseMoved(MouseEvent e) { - if (isWholeGenomeView()) { - for (ClickLink link : chromosomeRects) { - if (link.region.contains(e.getPoint())) { - setCursor(Cursor.getPredefinedCursor(Cursor.HAND_CURSOR)); - setToolTipText(link.tooltipText); - return; - } + for (ClickLink link : clickLinks) { + if (link.region.contains(e.getPoint())) { + setCursor(Cursor.getPredefinedCursor(Cursor.HAND_CURSOR)); + setToolTipText(link.tooltipText); + return; } - setCursor(Cursor.getDefaultCursor()); - setToolTipText(WHOLE_GENOME_TOOLTIP); - - } else { - for (MouseRect mr : mouseRects) { - if (mr.containsPoint(e.getPoint())) { - RulerPanel.this.setToolTipText(mr.getText()); - return; - } - } - RulerPanel.this.setToolTipText(CHROM_TOOLTIP); - } + setCursor(Cursor.getDefaultCursor()); + setToolTipText(isWholeGenomeView() ? WHOLE_GENOME_TOOLTIP : CHROM_TOOLTIP); + } @Override @@ -442,18 +500,18 @@ public void doMouseClick(MouseEvent evt) { setCursor(Cursor.getDefaultCursor()); WaitCursorManager.CursorToken token = WaitCursorManager.showWaitCursor(); try { - - if (!isWholeGenomeView()) { + boolean clickHandled = false; + for (final ClickLink link : clickLinks) { + if (link.region.contains(e.getPoint())) { + link.action.accept(link.value); + clickHandled = true; + } + } + if (!clickHandled && !isWholeGenomeView()) { double newLocation = frame.getChromosomePosition(e); frame.centerOnLocation(newLocation); - } else { - for (final ClickLink link : chromosomeRects) { - if (link.region.contains(e.getPoint())) { - final String chrName = link.value; - frame.changeChromosome(chrName, true); - } - } } + } finally { WaitCursorManager.removeWaitCursor(token); } @@ -564,38 +622,19 @@ public void setUnitMultiplier(int unitMultiplier) { class ClickLink { - Rectangle region; - String value; + Shape region; + Object value; String tooltipText; - ClickLink(Rectangle region, String value, String tooltipText) { + Consumer action; + + ClickLink(Shape region, Object value, String tooltipText, Consumer action) { this.region = region; this.value = value; this.tooltipText = tooltipText; - } - } - - - static class MouseRect { - Rectangle bounds; - String text; - - MouseRect(Rectangle bounds, String text) { - this.bounds = bounds; - this.text = text; + this.action = action; } - boolean containsPoint(Point p) { - return bounds.contains(p); - } - - String getText() { - return text; - } - - public int width() { - return bounds.width; - } } diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index e53c2799ea..deb27417a7 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -49,8 +49,8 @@ HOMREF.COLOR Homozygous reference color 235,235,235 HOMVAR.COLOR Homozygous variant color 0,245,255 HETVAR.COLOR Heterozygous variant color 0,0,255 NOCALL.COLOR No call color 255,255,255 -AF_REF.COLOR Allele freq - reference color 0,0,220 -AF_VAR.COLOR Allel freq - variant color 255,0,0 +AF_REF.COLOR Allele frequency - reference color 0,0,220 +AF_VAR.COLOR Allele frequency - variant color 255,0,0 VARIANT_COLOR_BY_ALLELE_FREQ Color by allele frequency (uncheck to color by allele fraction) boolean TRUE #Mutations @@ -123,7 +123,7 @@ SAM.FLAG_UNMAPPED_PAIR Flag unmapped pairs boolean FALSE SAM.SHOW_CENTER_LINE Show center line boolean FALSE SAM.SHOW_SOFT_CLIPPED Show soft-clipped bases boolean FALSE --- -SAM.SHADE_BASE_QUALITY Shade mismatched bases by quality. boolean FALSE +SAM.SHADE_BASE_QUALITY Shade mismatched bases by quality. boolean TRUE SAM.BASE_QUALITY_MIN Maximum transparency at base quality: integer 5 SAM.BASE_QUALITY_MAX No transparency above base quality: integer 20 --- diff --git a/src/test/java/org/broad/igv/sam/AlignmentDataManagerTest.java b/src/test/java/org/broad/igv/sam/AlignmentDataManagerTest.java index a5cfcad769..a052182476 100644 --- a/src/test/java/org/broad/igv/sam/AlignmentDataManagerTest.java +++ b/src/test/java/org/broad/igv/sam/AlignmentDataManagerTest.java @@ -369,7 +369,7 @@ public void testWithPadding() throws Exception{ * {@link AlignmentDataManager#loadInterval(String, int, int, AlignmentTrack.RenderOptions)} */ public static AlignmentInterval loadInterval(AlignmentDataManager manager, String chr, int start, int end) { - return manager.loadInterval(chr, start, end, (new AlignmentTrack.RenderOptions())); + return manager.loadInterval(chr, start, end, (new AlignmentTrack.RenderOptions(null))); } diff --git a/src/test/java/org/broad/igv/sam/AlignmentPackerTest.java b/src/test/java/org/broad/igv/sam/AlignmentPackerTest.java index ff473d300d..08b5acf735 100644 --- a/src/test/java/org/broad/igv/sam/AlignmentPackerTest.java +++ b/src/test/java/org/broad/igv/sam/AlignmentPackerTest.java @@ -82,7 +82,7 @@ public void testPackAlignments() throws Exception { */ AlignmentInterval interval = getAlignmentInterval(); - Map> result = (new AlignmentPacker()).packAlignments(interval, (new AlignmentTrack.RenderOptions())); + Map> result = (new AlignmentPacker()).packAlignments(interval, (new AlignmentTrack.RenderOptions(null))); assertEquals(1, result.size()); for (List alignmentrows : result.values()) { for (Row alignmentrow : alignmentrows) { @@ -105,7 +105,7 @@ public void testGroupAlignmentsPairOrientation() throws Exception { public Map> tstGroupAlignments(AlignmentTrack.GroupOption groupOption, int expSize) throws Exception { - AlignmentTrack.RenderOptions renderOptions = (new AlignmentTrack.RenderOptions()); + AlignmentTrack.RenderOptions renderOptions = (new AlignmentTrack.RenderOptions(null)); renderOptions.setGroupByOption(groupOption); AlignmentInterval interval = getAlignmentInterval(); From 47f33bbc6cba836d743493e6ae5d9696150082c9 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Sat, 26 Aug 2023 20:35:38 -0700 Subject: [PATCH 092/116] Update nucleotide color table if preferences change --- src/main/java/org/broad/igv/prefs/Constants.java | 8 ++++++++ src/main/java/org/broad/igv/prefs/IGVPreferences.java | 8 ++++++++ .../java/org/broad/igv/renderer/SequenceRenderer.java | 2 +- 3 files changed, 17 insertions(+), 1 deletion(-) diff --git a/src/main/java/org/broad/igv/prefs/Constants.java b/src/main/java/org/broad/igv/prefs/Constants.java index b284207b14..0abf7975c3 100644 --- a/src/main/java/org/broad/igv/prefs/Constants.java +++ b/src/main/java/org/broad/igv/prefs/Constants.java @@ -356,6 +356,14 @@ private Constants() { BASEMOD_NONE_N_COLOR ); + static java.util.List NUCLEOTIDE_COLOR_KEYS = Arrays.asList( + COLOR_A, + COLOR_C, + COLOR_G, + COLOR_N, + COLOR_T + ); + /** * List of keys that affect proxy usage */ diff --git a/src/main/java/org/broad/igv/prefs/IGVPreferences.java b/src/main/java/org/broad/igv/prefs/IGVPreferences.java index 77846c6c82..82733cbf17 100644 --- a/src/main/java/org/broad/igv/prefs/IGVPreferences.java +++ b/src/main/java/org/broad/igv/prefs/IGVPreferences.java @@ -40,6 +40,7 @@ import org.broad.igv.logging.Logger; import org.broad.igv.renderer.ColorScaleFactory; import org.broad.igv.renderer.ContinuousColorScale; +import org.broad.igv.renderer.SequenceRenderer; import org.broad.igv.sam.mods.BaseModificationColors; import org.broad.igv.track.TrackType; import org.broad.igv.ui.IGV; @@ -338,6 +339,13 @@ private void checkForAlignmentChanges(Map updatedPreferenceMap) } } + for (String key : NUCLEOTIDE_COLOR_KEYS) { + if (updatedPreferenceMap.containsKey(key)) { + SequenceRenderer.setNucleotideColors(); + break; + } + } + if (reloadSAM) { IGVEventBus.getInstance().post(new AlignmentTrackEvent(this, AlignmentTrackEvent.Type.RELOAD)); } diff --git a/src/main/java/org/broad/igv/renderer/SequenceRenderer.java b/src/main/java/org/broad/igv/renderer/SequenceRenderer.java index 5739fb589e..6d0b1c3a79 100644 --- a/src/main/java/org/broad/igv/renderer/SequenceRenderer.java +++ b/src/main/java/org/broad/igv/renderer/SequenceRenderer.java @@ -67,7 +67,7 @@ public static Map getNucleotideColors() { return nucleotideColors; } - private synchronized static void setNucleotideColors() { + public synchronized static void setNucleotideColors() { IGVPreferences prefs = PreferencesManager.getPreferences(); From 7ae949fff37dbbf25a73017ab434afc072590cd4 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Sat, 26 Aug 2023 20:38:25 -0700 Subject: [PATCH 093/116] expanded insertions -- (1) center view after collapsing insertion, (2) use subpixel rendering for zoomed out expanded insertions --- .../java/org/broad/igv/sam/BaseRenderer.java | 11 +++++++++-- .../org/broad/igv/ui/panel/ReferenceFrame.java | 16 ++++++++++------ 2 files changed, 19 insertions(+), 8 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/BaseRenderer.java b/src/main/java/org/broad/igv/sam/BaseRenderer.java index 84466c6579..60b296b565 100644 --- a/src/main/java/org/broad/igv/sam/BaseRenderer.java +++ b/src/main/java/org/broad/igv/sam/BaseRenderer.java @@ -7,6 +7,7 @@ import org.broad.igv.ui.color.ColorUtilities; import java.awt.*; +import java.awt.geom.Rectangle2D; import java.util.Collections; import java.util.HashMap; import java.util.List; @@ -69,6 +70,7 @@ public static void drawExpandedInsertions(InsertionMarker insertionMarker, Graphics2D g = null; try { g = (Graphics2D) context.getGraphics().create(); + g.setRenderingHint(RenderingHints.KEY_ANTIALIASING, RenderingHints.VALUE_ANTIALIAS_ON); double dX = 1 / context.getScale(); int fontSize = (int) Math.min(dX, 12); if (fontSize >= 8) { @@ -102,7 +104,7 @@ public static void drawExpandedInsertions(InsertionMarker insertionMarker, final int size = bases.length + padding; for (int p = 0; p < size; p++) { - int pX = (int) (pixelStart + (p / locScale)); + double pX = (pixelStart + (p / locScale)); // Don't draw out of clipping rect if (pX > rect.getMaxX()) break; @@ -118,7 +120,12 @@ public static void drawExpandedInsertions(InsertionMarker insertionMarker, color = BaseRenderer.getShadedColor(color, qual, renderOptions.getBaseQualityMin(), renderOptions.getBaseQualityMax()); } - drawBase(g, color, c, pX, rect.y, (int) dX, rect.height - (leaveMargin ? 2 : 0), false, null); + if(dX < 8) { + g.setColor(color); + g.fill(new Rectangle2D.Double(pX, rect.y, dX, rect.height)); + } else { + drawBase(g, color, c,(int) pX, rect.y, (int) dX, rect.height - (leaveMargin ? 2 : 0), false, null); + } } insertion.setPixelRange(pixelStart, pixelEnd); } diff --git a/src/main/java/org/broad/igv/ui/panel/ReferenceFrame.java b/src/main/java/org/broad/igv/ui/panel/ReferenceFrame.java index 25f3c36f16..e215a0da93 100644 --- a/src/main/java/org/broad/igv/ui/panel/ReferenceFrame.java +++ b/src/main/java/org/broad/igv/ui/panel/ReferenceFrame.java @@ -751,7 +751,17 @@ private static int getChromosomeLength(String chrName) { } } + public void setExpandedInsertion(InsertionMarker im) { + InsertionMarker previousInsertion = this.expandedInsertion; + this.expandedInsertion = im; + if(im == null && previousInsertion != null) { + this.centerOnLocation(previousInsertion.position); + } + } + public InsertionMarker getExpandedInsertion() { + return expandedInsertion; + } public int stateHash() { int result; long temp; @@ -771,12 +781,6 @@ private static Genome getGenome() { } - public void setExpandedInsertion(InsertionMarker im) { - this.expandedInsertion = im; - } - public InsertionMarker getExpandedInsertion() { - return expandedInsertion; - } } From 3917f972e28c501fdfd383cdc224432f01c48ad5 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Sat, 26 Aug 2023 22:26:56 -0700 Subject: [PATCH 094/116] Account for X translation when recording screen coordinates for features. Important in expanded insertion multi-frame rendering. --- .../java/org/broad/igv/sam/AlignmentRenderer.java | 12 +++++++----- src/main/java/org/broad/igv/sam/BaseRenderer.java | 4 ++-- src/main/java/org/broad/igv/track/RenderContext.java | 6 ++++++ .../org/broad/igv/ui/panel/DataPanelPainter.java | 2 +- 4 files changed, 16 insertions(+), 8 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java index 3d9fa781c0..ac041e70d9 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java +++ b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java @@ -654,7 +654,8 @@ private void drawAlignment( y, h, gapPxEnd - gapPxStart - 2, - null); + null, + context); } } } @@ -903,7 +904,8 @@ private void drawAlignment( y, h, (int) pxWidthExact, - aBlock); + aBlock, + context ); } else { int pxWing = (h > 10 ? 2 : (h > 5) ? 1 : 0); Graphics2D ig = context.getGraphics(); @@ -912,7 +914,7 @@ private void drawAlignment( ig.fillRect(x - pxWing, y, 2 + 2 * pxWing, 2); ig.fillRect(x - pxWing, y + h - 2, 2 + 2 * pxWing, 2); - aBlock.setPixelRange(x - pxWing, x + 2 + pxWing); + aBlock.setPixelRange(context.translateX + x - pxWing, context.translateX + x + 2 + pxWing); } } } @@ -1016,7 +1018,7 @@ private static void drawClippedEnds(final Graphics2D g, final int[] xPoly, final } private void drawLargeIndelLabel(Graphics2D g, boolean isInsertion, String labelText, int pxCenter, - int pxTop, int pxH, int pxWmax, AlignmentBlock insertionBlock) { + int pxTop, int pxH, int pxWmax, AlignmentBlock insertionBlock, RenderContext context) { final int pxPad = 2; // text padding in the label final int pxWing = (pxH > 10 ? 2 : 1); // width of the cursor "wing" @@ -1051,7 +1053,7 @@ private void drawLargeIndelLabel(Graphics2D g, boolean isInsertion, String label } // draw the text if it fits if (insertionBlock != null) { - insertionBlock.setPixelRange(pxLeft, pxRight); + insertionBlock.setPixelRange(context.translateX + pxLeft, context.translateX + pxRight); } } diff --git a/src/main/java/org/broad/igv/sam/BaseRenderer.java b/src/main/java/org/broad/igv/sam/BaseRenderer.java index 60b296b565..92e6b4d3d9 100644 --- a/src/main/java/org/broad/igv/sam/BaseRenderer.java +++ b/src/main/java/org/broad/igv/sam/BaseRenderer.java @@ -91,7 +91,7 @@ public static void drawExpandedInsertions(InsertionMarker insertionMarker, // Compute the start and end of the expanded insertion in pixels int pixelStart = (int) ((insertion.getStart() - origin) / locScale); - int pixelEnd = (int) ((insertion.getEnd() - origin) / locScale) + 1; + int pixelEnd = (int) (pixelStart + insertion.getLength() / locScale); // Skip if insertion is out of clipping rectangle -- this probably shouldn't happen if (pixelEnd < rect.x || pixelStart > rect.getMaxX()) { @@ -127,7 +127,7 @@ public static void drawExpandedInsertions(InsertionMarker insertionMarker, drawBase(g, color, c,(int) pX, rect.y, (int) dX, rect.height - (leaveMargin ? 2 : 0), false, null); } } - insertion.setPixelRange(pixelStart, pixelEnd); + insertion.setPixelRange(context.translateX + pixelStart, context.translateX + pixelEnd); } } } finally { diff --git a/src/main/java/org/broad/igv/track/RenderContext.java b/src/main/java/org/broad/igv/track/RenderContext.java index 0b9712bd17..b30f216a6b 100644 --- a/src/main/java/org/broad/igv/track/RenderContext.java +++ b/src/main/java/org/broad/igv/track/RenderContext.java @@ -55,6 +55,12 @@ public class RenderContext { public boolean multiframe = false; public int expandedInsertionPosition = -1; + /** + * X trasnlation for this context relative to its parent. This is used in expanded insertion "multi-frame* view + * to convert screen coordinates to parent reference system when recording the pixel location of drawn objects + */ + public int translateX = 0; + public RenderContext(JComponent panel, Graphics2D graphics, ReferenceFrame referenceFrame, Rectangle visibleRect) { this.graphics = graphics; this.panel = panel; diff --git a/src/main/java/org/broad/igv/ui/panel/DataPanelPainter.java b/src/main/java/org/broad/igv/ui/panel/DataPanelPainter.java index 93a96cdc0d..144d5d9757 100644 --- a/src/main/java/org/broad/igv/ui/panel/DataPanelPainter.java +++ b/src/main/java/org/broad/igv/ui/panel/DataPanelPainter.java @@ -119,9 +119,9 @@ private RenderContext shiftRenderContext(RenderContext ctx, double position, int newContext.getReferenceFrame().widthInPixels = pixelWidth; newContext.getReferenceFrame().origin = position; newContext.visibleRect = new Rectangle(0, ctx.visibleRect.y, pixelWidth, ctx.visibleRect.height); + newContext.translateX = translateX; Graphics2D dG = newContext.getGraphics(); - Rectangle dRect = newContext.visibleRect; dG.translate(translateX, 0); dG.setClip(newContext.visibleRect); From e9a20ea099b41be063382e6e96dbaaff738b65fe Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Sun, 27 Aug 2023 21:24:16 -0700 Subject: [PATCH 095/116] Reorganize from alignment preferences. --- .../org/broad/igv/prefs/preferences.tab | 39 ++++++++++++------- 1 file changed, 24 insertions(+), 15 deletions(-) diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index deb27417a7..68f316b430 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -83,28 +83,35 @@ CN_FREQ.AMP_THRESHOLD CN freq amplification threshold float 0.1 CN_FREQ.DEL_THRESHOLD CN freq deletion threshold float -0.1 #Alignments -info Default settings for alignment tracks. A subset of these properties are overriden for RNA and 3rd generation data. -info See the RNA or Third Gen tabs for those track types. +info Default settings for alignment tracks. A subset of these properties can be overriden for RNA and 3rd generation data. +info See also the RNA and Third Gen tabs. ##Track Display SAM.SHOW_ALIGNMENT_TRACK Show alignment track boolean TRUE SAM.SHOW_COV_TRACK Show coverage track boolean TRUE SAM.SHOW_JUNCTION_TRACK Show junction track boolean FALSE +SAM.SHOW_INSERTION_MARKERS Show insertion markers boolean FALSE +SAM.MAX_VISIBLE_RANGE Visibility range threshold (kb) float 30 Range at which alignments become visibile ##Downsampling SAM.DOWNSAMPLE_READS Downsample reads boolean TRUE SAM.SAMPLING_WINDOW Sampling window size (bases) integer 50 SAM.MAX_LEVELS Number of reads per window integer 100 +##Coverage Track Options +SAM.ALLELE_THRESHOLD Coverage allele-fraction threshold float 0.2 +SAM.ALLELE_USE_QUALITY Quality weight allele fraction boolean TRUE + ##Alignment Track Options + SAM.COLOR_BY Color alignments by select NONE|READ_STRAND|FIRST_OF_PAIR_STRAND|PAIR_ORIENTATION|UNEXPECTED_PAIR|INSERT_SIZE|SAMPLE|READ_GROUP|LIBRARY|MOVIE|ZMW|BISULFITE|NOMESEQ|TAG|MAPPED_SIZE|LINK_STRAND|YC_TAG UNEXPECTED_PAIR SAM.COLOR_BY_TAG Color by TAG string SAM.GROUP_OPTION Group alignments by select NONE|STRAND|SAMPLE|READ_GROUP|LIBRARY|FIRST_OF_PAIR_STRAND|TAG|PAIR_ORIENTATION|MATE_CHROMOSOME|SV_ALIGNMENT|SUPPLEMENTARY|BASE_AT_POS|MOVIE|ZMW|HAPLOTYPE|READ_ORDER|LINKED|PHASE|MAPPING_QUALITY SAM.GROUP_BY_TAG Group by TAG string SAM.GROUP_ALL Syncrohize alignment track grouping boolean FALSE -SAM.MAX_VISIBLE_RANGE Visibility range threshold (kb) float 30 Range at which alignments become visibile + SAM.QUALITY_THRESHOLD Mapping quality threshold int 0 Hide alignments with MQ lower than this value -SAM.ALIGNMENT_SCORE_THRESHOLD Alignment score threshold int 0 +SAM.ALIGNMENT_SCORE_THRESHOLD Alignment score threshold (AS tag) int 0 SAM.DISPLAY_MODE Alignment display mode string EXPANDED SAM.DISPLAY_PAIRED Display reads as paired by default boolean FALSE --- @@ -138,20 +145,17 @@ SAM.CLIPPING_THRESHOLD Flag clipping threshold (bases) integer 0 SAM.SHOW_CONNECTED_CHR_NAME Show connected chromosome name boolean TRUE --- SAM.QUICK_CONSENSUS_MODE Quick consensus mode boolean FALSE -SAM.SHOW_INSERTION_MARKERS Show insertion markers boolean FALSE + --- SAM.HIDDEN_TAGS Hide tags in popup text (comma delimited list) string SA,MD,XA,RG --- SAM.LINK_BY_TAGS Tags to be used for linking alignments (comma delimited list) string --- -SAM.MAX_SOFT_CLIP Soft clip reference extension (reference sequence is extended by this amount to show soft clips) integer 1000 +SAM.MAX_SOFT_CLIP Soft clip reference extension maximum integer 1000 Reference sequence is extended up to this amount to show soft clips overhanging reference edge. --- SAM.FILTER_ALIGNMENTS Filter alignments by read group boolean FALSE SAM.FILTER_URL URL or path to read group filter file string null -##Coverage Track Options -SAM.ALLELE_THRESHOLD Coverage allele-fraction threshold float 0.2 -SAM.ALLELE_USE_QUALITY Quality weight allele fraction boolean TRUE ##Insert Size Options SAM.COMPUTE_ISIZES Compute insert size thresholds boolean TRUE @@ -167,7 +171,7 @@ info Settings below override defaults for RNA alignments. SAM.SHOW_ALIGNMENT_TRACK Show alignment track boolean TRUE SAM.SHOW_COV_TRACK Show coverage track boolean TRUE SAM.SHOW_JUNCTION_TRACK Show junction track boolean TRUE - +SAM.SHOW_INSERTION_MARKERS Show insertion markers boolean FALSE SAM.MAX_VISIBLE_RANGE Visibility range threshold (kb) float 300 Range at which alignments become visibile ##Splice Junction Track @@ -185,23 +189,28 @@ SAM.INSERT_SIZE_THRESHOLD Default maximum (bp) float 100000 #Third Gen THIRD_GEN info Settings below override defaults for 3rd-gen (PacBio, Oxford Nanopore, ...) alignments. + SAM.MAX_VISIBLE_RANGE Visibility range threshold (kb) float 1000 Range at which alignments become visibile +SAM.SHOW_INSERTION_MARKERS Show insertion markers boolean TRUE +SAM.QUICK_CONSENSUS_MODE Quick consensus mode boolean TRUE ##Downsampling SAM.DOWNSAMPLE_READS Downsample reads boolean FALSE +SAM.SAMPLING_WINDOW Sampling window size (bases) integer 50 +SAM.MAX_LEVELS Number of reads per window integer 100 +##Indels SAM.FLAG_LARGE_INDELS Label indels > label threshold boolean TRUE SAM.LARGE_INSERTIONS_THRESOLD Label threshold (bases) integer 1 --- SAM.HIDE_SMALL_INDEL Hide indels < show indel threshold boolean TRUE SAM.SMALL_INDEL_BP_THRESHOLD Show indel threshold (bases) integer 2 ---- + +##Clipping SAM.FLAG_CLIPPING Flag clipping > flag clipping threshold boolean TRUE SAM.CLIPPING_THRESHOLD Flag clipping threshold (bases) integer 20 ---- -SAM.QUICK_CONSENSUS_MODE Quick consensus mode boolean TRUE -SAM.SHOW_INSERTION_MARKERS Show insertion markers boolean TRUE ---- + +##Linking SAM.LINK_READS Link alignments by tag boolean FALSE SAM.LINK_TAG Linking tag string READNAME From 818c809792e06fb149946dd6e876e528d488eace Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Tue, 29 Aug 2023 22:50:05 -0700 Subject: [PATCH 096/116] Fix broken sashimi plot Filter sashimi plot menu to RNA-Seq experiments --- .../org/broad/igv/sam/AlignmentTrack.java | 2 +- .../org/broad/igv/sam/AlignmentTrackMenu.java | 3 ++- .../broad/igv/sam/SpliceJunctionTrack.java | 6 +++++ .../org/broad/igv/sashimi/SashimiPlot.java | 22 ++++++++++++------- 4 files changed, 23 insertions(+), 10 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrack.java b/src/main/java/org/broad/igv/sam/AlignmentTrack.java index 0af84ef622..1c8fa08b10 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrack.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrack.java @@ -423,7 +423,7 @@ void setExperimentType(ExperimentType type) { } } - ExperimentType getExperimentType() { + public ExperimentType getExperimentType() { return experimentType; } diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index 25fa117d86..26cbe2dff9 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -162,10 +162,11 @@ class AlignmentTrackMenu extends IGVPopupMenu { addInsertionItems(insertion); } - // Sashimi plot, probably should be depdenent on experimentType (RNA) + // Sashimi plot addSeparator(); JMenuItem sashimi = new JMenuItem("Sashimi Plot"); sashimi.addActionListener(e1 -> SashimiPlot.openSashimiPlot()); + sashimi.setEnabled(alignmentTrack.getExperimentType() == AlignmentTrack.ExperimentType.RNA); add(sashimi); // Show alignments, coverage, splice junctions diff --git a/src/main/java/org/broad/igv/sam/SpliceJunctionTrack.java b/src/main/java/org/broad/igv/sam/SpliceJunctionTrack.java index be6ddec058..fa0902dbe9 100644 --- a/src/main/java/org/broad/igv/sam/SpliceJunctionTrack.java +++ b/src/main/java/org/broad/igv/sam/SpliceJunctionTrack.java @@ -33,6 +33,7 @@ import org.broad.igv.feature.SpliceJunctionFeature; import org.broad.igv.renderer.DataRange; import org.broad.igv.renderer.GraphicUtils; +import org.broad.igv.renderer.Renderer; import org.broad.igv.renderer.SpliceJunctionRenderer; import org.broad.igv.track.*; import org.broad.igv.ui.IGV; @@ -95,6 +96,11 @@ public SpliceJunctionTrack() { this.renderer = new SpliceJunctionRenderer(); } + @Override + public void setRenderer(Renderer renderer) { + this.renderer = renderer; + } + @Override public String getSample() { if (sampleId != null) { diff --git a/src/main/java/org/broad/igv/sashimi/SashimiPlot.java b/src/main/java/org/broad/igv/sashimi/SashimiPlot.java index 78e7f1b33e..c0211c34e5 100644 --- a/src/main/java/org/broad/igv/sashimi/SashimiPlot.java +++ b/src/main/java/org/broad/igv/sashimi/SashimiPlot.java @@ -49,6 +49,7 @@ import java.io.File; import java.util.List; import java.util.*; +import java.util.stream.Collectors; /** * Window for displaying sashimi style junction plot @@ -81,6 +82,8 @@ public class SashimiPlot extends JFrame implements IGVEventObserver { private static final List plotColors; + private Map junctionRendererMap; + static { ColorPalette palette = ColorUtilities.getDefaultPalette(); plotColors = Arrays.asList(palette.getColors()); @@ -110,8 +113,11 @@ public SashimiPlot(ReferenceFrame iframe, Collection a sashimiPanel.add(generateControlPanel(this.referenceFrame)); spliceJunctionTracks = new ArrayList<>(alignmentTracks.size()); + int colorInd = 0; + junctionRendererMap = new HashMap<>(); + eventBus.subscribe(ViewChange.class, this); for (AlignmentTrack alignmentTrack : alignmentTracks) { @@ -125,7 +131,9 @@ public SashimiPlot(ReferenceFrame iframe, Collection a // Override expand/collpase setting -- expanded sashimi plots make no sense spliceJunctionTrack.setDisplayMode(Track.DisplayMode.COLLAPSED); - spliceJunctionTrack.setRenderer(new SashimiJunctionRenderer()); + SashimiJunctionRenderer renderer = new SashimiJunctionRenderer(); + spliceJunctionTrack.setRenderer(renderer); + junctionRendererMap.put(spliceJunctionTrack, renderer); Color color = plotColors.get(colorInd); colorInd = (colorInd + 1) % plotColors.size(); @@ -234,7 +242,7 @@ private void initSpliceJunctionComponent(TrackComponent tra } private SashimiJunctionRenderer getRenderer(SpliceJunctionTrack spliceJunctionTrack) { - return (SashimiJunctionRenderer) spliceJunctionTrack.getRenderer(); + return junctionRendererMap.get(spliceJunctionTrack); } @Override @@ -649,13 +657,11 @@ public static void openSashimiPlot() { geneTrack = dlg.getSelectedTrack(); } - Collection alignmentTracks = new ArrayList(); - for (Track track : IGV.getInstance().getAllTracks()) { - if (track instanceof AlignmentTrack) { - alignmentTracks.add((AlignmentTrack) track); - } - } + + Collection alignmentTracks =IGV.getInstance().getAlignmentTracks().stream() + .filter(t -> t.getExperimentType() == AlignmentTrack.ExperimentType.RNA).collect(Collectors.toList()); +; if (alignmentTracks.size() > 1) { TrackSelectionDialog alDlg = new TrackSelectionDialog(IGV.getInstance().getMainFrame(), TrackSelectionDialog.SelectionMode.MULTIPLE, alignmentTracks); From 361cde8b96349fbdfee2bec00579e9017220f134 Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Wed, 30 Aug 2023 10:32:03 -0700 Subject: [PATCH 097/116] Feature refactor (#1382) * Introduce whole-genome feature class * Rename method names for clarity * Use 1 fully closed coordinate system for ".mut" file parsing. Fixes #1226 (#1229) * implement tabix and csi indeces -- ports from igv.js --- src/main/java/module-info.java | 2 + .../org/broad/igv/bbfile/BBDataSource.java | 2 +- .../java/org/broad/igv/data/DataSource.java | 7 +- .../igv/data/seg/SegmentedDataSource.java | 5 - .../org/broad/igv/feature/AbstractCacher.java | 365 ------ .../igv/feature/CachingFeatureSource.java | 93 -- .../org/broad/igv/feature/IGVFeature.java | 4 +- .../java/org/broad/igv/feature/WGFeature.java | 57 + .../igv/feature/gff/GFFFeatureSource.java | 4 +- .../igv/feature/tribble/IGVFeatureReader.java | 16 +- .../broad/igv/feature/tribble/MUTCodec.java | 10 +- .../igv/feature/tribble/reader/BamIndex.java | 310 +++++ .../igv/feature/tribble/reader/CSIIndex.java | 328 ++++++ .../igv/feature/tribble/reader/VPointer.java | 29 + .../broad/igv/htsget/HtsgetVariantSource.java | 2 +- .../broad/igv/session/IGVSessionReader.java | 2 + .../tools/motiffinder/MotifFinderPlugin.java | 5 - .../tools/motiffinder/MotifFinderSource.java | 5 +- .../igv/track/FeatureCollectionSource.java | 28 +- .../org/broad/igv/track/FeatureSource.java | 22 +- .../org/broad/igv/track/FeatureTrack.java | 124 +- .../java/org/broad/igv/track/MotifTrack.java | 6 +- .../org/broad/igv/track/MutationTrack.java | 78 -- .../broad/igv/track/TribbleFeatureSource.java | 19 +- .../igv/track/TribbleListFeatureSource.java | 2 +- .../org/broad/igv/variant/New/Variant.java | 19 +- .../org/broad/igv/prefs/preferences.tab | 2 + .../feature/tribble/reader/BamIndexTest.java | 171 +++ .../feature/tribble/reader/CSIIndexTest.java | 94 ++ test/data/tabix/TSVC.vcf.gz | Bin 0 -> 4833 bytes test/data/tabix/TSVC.vcf.gz.csi | Bin 0 -> 140 bytes test/data/tabix/TSVC.vcf.gz.tbi | Bin 0 -> 135 bytes test/data/tabix/csi-test.vcf | 1001 +++++++++++++++++ test/data/tabix/csi-test.vcf.gz | Bin 0 -> 94832 bytes test/data/tabix/csi-test.vcf.gz.csi | Bin 0 -> 326 bytes test/data/tabix/index_test.bam.csi | Bin 0 -> 48824 bytes test/data/tabix/long_references.bam.csi | Bin 0 -> 133 bytes test/data/tabix/noindex.chr1.gff.gz | Bin 0 -> 313 bytes test/data/tabix/refGene.hg19.bed.gz.tbi | Bin 0 -> 106571 bytes test/data/tabix/sorted.genes.gtf.gz | Bin 0 -> 5207306 bytes test/data/tabix/sorted.genes.gtf.gz.csi | Bin 0 -> 37288 bytes test/data/tabix/sorted.genes.gtf.gz.tbi | Bin 0 -> 245605 bytes test/data/tabix/uncompressed_index.bam.csi | Bin 0 -> 6623684 bytes 43 files changed, 2146 insertions(+), 666 deletions(-) delete mode 100644 src/main/java/org/broad/igv/feature/AbstractCacher.java delete mode 100644 src/main/java/org/broad/igv/feature/CachingFeatureSource.java create mode 100644 src/main/java/org/broad/igv/feature/WGFeature.java create mode 100644 src/main/java/org/broad/igv/feature/tribble/reader/BamIndex.java create mode 100644 src/main/java/org/broad/igv/feature/tribble/reader/CSIIndex.java create mode 100644 src/main/java/org/broad/igv/feature/tribble/reader/VPointer.java create mode 100644 src/test/java/org/broad/igv/feature/tribble/reader/BamIndexTest.java create mode 100644 src/test/java/org/broad/igv/feature/tribble/reader/CSIIndexTest.java create mode 100755 test/data/tabix/TSVC.vcf.gz create mode 100644 test/data/tabix/TSVC.vcf.gz.csi create mode 100755 test/data/tabix/TSVC.vcf.gz.tbi create mode 100644 test/data/tabix/csi-test.vcf create mode 100644 test/data/tabix/csi-test.vcf.gz create mode 100644 test/data/tabix/csi-test.vcf.gz.csi create mode 100644 test/data/tabix/index_test.bam.csi create mode 100644 test/data/tabix/long_references.bam.csi create mode 100644 test/data/tabix/noindex.chr1.gff.gz create mode 100644 test/data/tabix/refGene.hg19.bed.gz.tbi create mode 100644 test/data/tabix/sorted.genes.gtf.gz create mode 100644 test/data/tabix/sorted.genes.gtf.gz.csi create mode 100644 test/data/tabix/sorted.genes.gtf.gz.tbi create mode 100644 test/data/tabix/uncompressed_index.bam.csi diff --git a/src/main/java/module-info.java b/src/main/java/module-info.java index 15bb2a73f2..9e822e87a7 100644 --- a/src/main/java/module-info.java +++ b/src/main/java/module-info.java @@ -5,8 +5,10 @@ exports org.broad.igv.jbrowse; exports org.broad.igv.logging; exports org.broad.igv.util.liftover; + exports org.broad.igv.feature; exports org.broad.igv.sam.smrt; + requires com.google.common; requires commons.math3; requires gson; diff --git a/src/main/java/org/broad/igv/bbfile/BBDataSource.java b/src/main/java/org/broad/igv/bbfile/BBDataSource.java index 2d19355227..36c609e16e 100644 --- a/src/main/java/org/broad/igv/bbfile/BBDataSource.java +++ b/src/main/java/org/broad/igv/bbfile/BBDataSource.java @@ -111,7 +111,7 @@ public BBDataSource(BBFileReader reader, Genome genome) throws IOException { } @Override - public int getFeatureWindowSize() { + public int estimateFeatureWindowSize() { return featureVisiblityWindow; } diff --git a/src/main/java/org/broad/igv/data/DataSource.java b/src/main/java/org/broad/igv/data/DataSource.java index b4efe3778b..3df6293ffa 100644 --- a/src/main/java/org/broad/igv/data/DataSource.java +++ b/src/main/java/org/broad/igv/data/DataSource.java @@ -60,15 +60,12 @@ List getSummaryScoresForRange( boolean isLogNormalized(); - public WindowFunction getWindowFunction(); + WindowFunction getWindowFunction(); - public default Collection getAvailableWindowFunctions() { + default Collection getAvailableWindowFunctions() { return Collections.EMPTY_LIST; } - default boolean isIndexable() { - return true; - } /** * Release any resources (file handles, etc) diff --git a/src/main/java/org/broad/igv/data/seg/SegmentedDataSource.java b/src/main/java/org/broad/igv/data/seg/SegmentedDataSource.java index 654bd4829e..6beed7ad31 100644 --- a/src/main/java/org/broad/igv/data/seg/SegmentedDataSource.java +++ b/src/main/java/org/broad/igv/data/seg/SegmentedDataSource.java @@ -74,11 +74,6 @@ public void setWindowFunction(WindowFunction statType) { // ignore } - @Override - public boolean isIndexable() { - return false; - } - public boolean isLogNormalized() { return dataset.isLogNormalized(); } diff --git a/src/main/java/org/broad/igv/feature/AbstractCacher.java b/src/main/java/org/broad/igv/feature/AbstractCacher.java deleted file mode 100644 index f7852565a2..0000000000 --- a/src/main/java/org/broad/igv/feature/AbstractCacher.java +++ /dev/null @@ -1,365 +0,0 @@ -/* - * The MIT License (MIT) - * - * Copyright (c) 2007-2015 Broad Institute - * - * Permission is hereby granted, free of charge, to any person obtaining a copy - * of this software and associated documentation files (the "Software"), to deal - * in the Software without restriction, including without limitation the rights - * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell - * copies of the Software, and to permit persons to whom the Software is - * furnished to do so, subject to the following conditions: - * - * The above copyright notice and this permission notice shall be included in - * all copies or substantial portions of the Software. - * - * - * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR - * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, - * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE - * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER - * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, - * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN - * THE SOFTWARE. - */ - - -package org.broad.igv.feature; - -import org.broad.igv.logging.*; -import org.broad.igv.util.collections.LRUCache; -import htsjdk.tribble.CloseableTribbleIterator; -import htsjdk.tribble.Feature; - -import java.io.IOException; -import java.util.ArrayList; -import java.util.Collections; -import java.util.Iterator; -import java.util.List; - - -/** - * Class to handle caching data from any source of features - * Subclasses must override queryRaw - * - * @author jrobinso - * @date Jun 24, 2010 - */ -public abstract class AbstractCacher { - - private static Logger log = LogManager.getLogger(AbstractCacher.class); - - protected int binSize = Integer.MAX_VALUE; - protected LRUCache cache; - - - public AbstractCacher(int binCount, int binSize) { - this.cache = new LRUCache(binCount); - setBinSize(binSize); - } - - /** - * Obtain data from underlying source - * - * @param chr - * @param start - * @param end - * @return - */ - protected abstract Iterator queryRaw(String chr, int start, int end) throws IOException; - - - - /** - * Set the bin size. This invalidates the cache. - * - * @param newSize - */ - public void setBinSize(int newSize) { - this.binSize = newSize == 0 ? Integer.MAX_VALUE : newSize; // A binSize of zero => use a single bin for the entire chromosome - cache.clear(); - - } - - public void close() { - cache.clear(); - } - - /** - * Query the cached data, refreshing from raw data as necessary - * - * @param chr - * @param start - * @param end - * @return - * @throws IOException - */ - public Iterator queryCached(String chr, int start, int end) throws IOException { - - int startBin = start / binSize; - int endBin = end / binSize; // <= inclusive - - List tiles = getBins(chr, startBin, endBin); - - if (tiles.size() == 0) { - return Collections.emptyList().iterator(); - } - - // Count total # of records - int recordCount = tiles.get(0).getOverlappingRecords().size(); - for (Bin t : tiles) { - recordCount += t.getContainedRecords().size(); - } - - List features = new ArrayList(recordCount); - features.addAll(tiles.get(0).getOverlappingRecords()); - for (Bin t : tiles) { - features.addAll(t.getContainedRecords()); - } - return new BinIterator(start, end, features); - } - - - /** - * Return loaded tiles that span the query interval. - *

- * We synchronize this method because different threads might be using - * the same source. Synchronizing here ensures that data - * is loaded as few times as possible (first caller loads it into the cache, - * the second caller accesses it from there) as well as preventing bugs stemming - * from multiple thread access - * - * @param seq - * @param startBin - * @param endBin - * @return - */ - private synchronized List getBins(String seq, int startBin, int endBin) { - - List tiles = new ArrayList(endBin - startBin + 1); - List tilesToLoad = new ArrayList(endBin - startBin + 1); - - for (int t = startBin; t <= endBin; t++) { - String key = seq + "_" + t; - Bin tile = cache.get(key); - - if (tile == null) { - if (log.isDebugEnabled()) { - log.debug("Tile cache miss: " + t); - } - int start = t * binSize; - int end = start + binSize; - tile = new Bin(t, start, end); - cache.put(key, tile); - } - - tiles.add(tile); - - // The current tile is loaded, load any preceding tiles we have pending - if (tile.isLoaded()) { - if (tilesToLoad.size() > 0) { - if (!loadTiles(seq, tilesToLoad)) { - return tiles; - } - } - tilesToLoad.clear(); - } else { - tilesToLoad.add(tile); - } - } - - if (tilesToLoad.size() > 0) { - loadTiles(seq, tilesToLoad); - } - - return tiles; - } - - private boolean loadTiles(String seq, List tiles) { - - assert (tiles.size() > 0); - - if (log.isDebugEnabled()) { - int first = tiles.get(0).getBinNumber(); - int end = tiles.get(tiles.size() - 1).getBinNumber(); - log.debug("Loading tiles: " + first + "-" + end); - } - - // Convert start to 1-based coordinates - int start = tiles.get(0).start + 1; - int end = tiles.get(tiles.size() - 1).end; - Iterator iter = null; - - //log.debug("Loading : " + start + " - " + end); - int featureCount = 0; - long t0 = System.currentTimeMillis(); - try { - - iter = queryRaw(seq, start, end); - - while (iter != null && iter.hasNext()) { - Feature record = iter.next(); - - // Range of tile indices that this feature contributes to. - int aStart = record.getStart(); - int aEnd = record.getEnd(); - int idx0 = 0; - int idx1 = 0; - if (binSize > 0) { - idx0 = Math.max(0, (aStart - start) / binSize); - idx1 = Math.min(tiles.size() - 1, (aEnd - start) / binSize); - } - - // Loop over tiles this read overlaps - for (int i = idx0; i <= idx1; i++) { - Bin t = tiles.get(i); - - // A bin size == 0 means use a single bin for the entire chromosome. This is a confusing convention. - if (binSize == 0 || ((aStart >= t.start) && (aStart < t.end))) { - t.containedRecords.add(record); - } else if ((aEnd >= t.start) && (aStart < t.start)) { - t.overlappingRecords.add(record); - } - } - } - - for (Bin t : tiles) { - t.setLoaded(true); - } - if (log.isDebugEnabled()) { - long dt = System.currentTimeMillis() - t0; - long rate = dt == 0 ? Long.MAX_VALUE : featureCount / dt; - log.debug("Loaded " + featureCount + " reads in " + dt + "ms. (" + rate + " reads/ms)"); - } - return true; - - } catch (IOException e) { - log.error("IOError loading feature data", e); - - // TODO -- do something about this, how do we want to handle this exception? - throw new RuntimeException(e); - } finally { - if (iter != null) { - //iter.close(); - } - //IGV.getInstance().resetStatusMessage(); - } - } - - - private static class Bin { - - private boolean loaded = false; - private int start; - private int end; - private int binNumber; - private List containedRecords; - private List overlappingRecords; - - Bin(int binNumber, int start, int end) { - this.binNumber = binNumber; - this.start = start; - this.end = end; - containedRecords = new ArrayList(1000); - overlappingRecords = new ArrayList(100); - } - - public int getBinNumber() { - return binNumber; - } - - - public int getStart() { - return start; - } - - public void setStart(int start) { - this.start = start; - } - - public List getContainedRecords() { - return containedRecords; - } - - public List getOverlappingRecords() { - return overlappingRecords; - } - - public boolean isLoaded() { - return loaded; - } - - public void setLoaded(boolean loaded) { - this.loaded = loaded; - } - - } - - /** - * - */ - private class BinIterator implements CloseableTribbleIterator { - - Iterator currentFeatureIterator; - int end; - Feature nextRecord; - int start; - List features; - - BinIterator(int start, int end, List features) { - this.features = features; - this.start = start; - this.end = end; - currentFeatureIterator = features.iterator(); - advanceToFirstRecord(); - } - - public void close() { - // No-op - } - - public boolean hasNext() { - return nextRecord != null; - } - - public Feature next() { - Feature ret = nextRecord; - - advanceToNextRecord(); - - return ret; - } - - public void remove() { - // ignored - } - - private void advanceToFirstRecord() { - advanceToNextRecord(); - } - - private void advanceToNextRecord() { - advance(); - - while ((nextRecord != null) && (nextRecord.getEnd() < start)) { - advance(); - } - } - - private void advance() { - if (currentFeatureIterator.hasNext()) { - nextRecord = currentFeatureIterator.next(); - if (nextRecord.getStart() > end) { - nextRecord = null; - } - } else { - nextRecord = null; - } - } - - public Iterator iterator() { - return this; - } - } -} - diff --git a/src/main/java/org/broad/igv/feature/CachingFeatureSource.java b/src/main/java/org/broad/igv/feature/CachingFeatureSource.java deleted file mode 100644 index 407b92486a..0000000000 --- a/src/main/java/org/broad/igv/feature/CachingFeatureSource.java +++ /dev/null @@ -1,93 +0,0 @@ -/* - * The MIT License (MIT) - * - * Copyright (c) 2007-2015 Broad Institute - * - * Permission is hereby granted, free of charge, to any person obtaining a copy - * of this software and associated documentation files (the "Software"), to deal - * in the Software without restriction, including without limitation the rights - * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell - * copies of the Software, and to permit persons to whom the Software is - * furnished to do so, subject to the following conditions: - * - * The above copyright notice and this permission notice shall be included in - * all copies or substantial portions of the Software. - * - * - * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR - * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, - * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE - * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER - * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, - * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN - * THE SOFTWARE. - */ - -package org.broad.igv.feature; - -import org.broad.igv.track.FeatureSource; -import htsjdk.tribble.Feature; - -import java.io.IOException; -import java.util.Iterator; -import java.util.List; - -/** - * A FeatureSource wrapper which provides caching. - * The cache is only cleared when the source is closed. - * - * @author jacob - * @date 2012/05/15 - */ -public class CachingFeatureSource extends AbstractCacher implements FeatureSource { - - private static final int maxBinCount = 1000; - private static final int defaultBinSize = 16000; // <= 16 kb - - private FeatureSource source; - - - /** - * Wraps the provided {@code source} with a caching version, - * using default parameters. - * @param source - * @api - */ - public CachingFeatureSource(FeatureSource source) { - this(source, maxBinCount, defaultBinSize); - } - - - public CachingFeatureSource(FeatureSource source, int binCount, int binSize) { - super(binCount, binSize); - this.source = source; - } - - @Override - protected Iterator queryRaw(String chr, int start, int end) throws IOException { - return source.getFeatures(chr, start, end); - } - - @Override - public Iterator getFeatures(String chr, int start, int end) throws IOException { - return super.queryCached(chr, start, end); - } - - @Override - public List getCoverageScores(String chr, int start, int end, int zoom) { - return source.getCoverageScores(chr, start, end, zoom); - } - - @Override - public int getFeatureWindowSize() { - return source.getFeatureWindowSize(); - } - - /** - * Return the source which backs this CachingFeatureSource - * @return - */ - public FeatureSource getSource(){ - return this.source; - } -} diff --git a/src/main/java/org/broad/igv/feature/IGVFeature.java b/src/main/java/org/broad/igv/feature/IGVFeature.java index 0852468bd3..79134f8f8f 100644 --- a/src/main/java/org/broad/igv/feature/IGVFeature.java +++ b/src/main/java/org/broad/igv/feature/IGVFeature.java @@ -35,7 +35,7 @@ * Interface for features in IGV annotation tracks (FeatureTrack and derived classes). */ -public interface IGVFeature extends LocusScore, NamedFeature { +public interface IGVFeature extends LocusScore, NamedFeature, Cloneable { default String getIdentifier() { return null; @@ -90,7 +90,7 @@ default List getExons() { return null; } - public Color getColor(); + Color getColor(); default String getURL() { return null; diff --git a/src/main/java/org/broad/igv/feature/WGFeature.java b/src/main/java/org/broad/igv/feature/WGFeature.java new file mode 100644 index 0000000000..74bf44e46a --- /dev/null +++ b/src/main/java/org/broad/igv/feature/WGFeature.java @@ -0,0 +1,57 @@ +package org.broad.igv.feature; + +import org.broad.igv.Globals; +import org.broad.igv.feature.genome.GenomeManager; +import org.broad.igv.track.WindowFunction; + +import java.awt.*; + +public class WGFeature implements IGVFeature { + + IGVFeature wrappedFeature; + int start; + int end; + + public WGFeature(IGVFeature wrappedFeature, int start, int end) { + this.wrappedFeature = wrappedFeature; + this.start = start; + this.end = end; + } + + + @Override + public Color getColor() { + return wrappedFeature.getColor(); + } + + @Override + public float getScore() { + return wrappedFeature.getScore(); + } + + @Override + public String getName() { + return wrappedFeature.getName(); + } + + @Override + public String getContig() { + return Globals.CHR_ALL; + } + + @Override + public int getStart() { + return start; + } + + @Override + public int getEnd() { + return end; + } + + @Override + public String getValueString(double position, int mouseX, WindowFunction windowFunction) { + // TODDO -- translate position to chr position + return wrappedFeature.getValueString(0, mouseX, windowFunction); + } +} diff --git a/src/main/java/org/broad/igv/feature/gff/GFFFeatureSource.java b/src/main/java/org/broad/igv/feature/gff/GFFFeatureSource.java index ff9da6d7e6..eb72a50188 100644 --- a/src/main/java/org/broad/igv/feature/gff/GFFFeatureSource.java +++ b/src/main/java/org/broad/igv/feature/gff/GFFFeatureSource.java @@ -105,8 +105,8 @@ public List getCoverageScores(String chr, int start, int end, int zo } @Override - public int getFeatureWindowSize() { - return wrappedSource.getFeatureWindowSize(); + public int estimateFeatureWindowSize() { + return wrappedSource.estimateFeatureWindowSize(); } } diff --git a/src/main/java/org/broad/igv/feature/tribble/IGVFeatureReader.java b/src/main/java/org/broad/igv/feature/tribble/IGVFeatureReader.java index da793e7fa7..8449de024a 100644 --- a/src/main/java/org/broad/igv/feature/tribble/IGVFeatureReader.java +++ b/src/main/java/org/broad/igv/feature/tribble/IGVFeatureReader.java @@ -26,8 +26,6 @@ package org.broad.igv.feature.tribble; - - import htsjdk.tribble.Feature; import htsjdk.tribble.index.Index; @@ -38,21 +36,21 @@ /** * Interface to replace the Tribble equivalent. Returns plain "Iterator" rather than "CloseableIterator". A wrapper * implementation is provided that handles closing the tribble resource. - * + *

* NOTE: IGV classes should use this interface, rather than the Tribble FeatureReader. * * @author jrobinso - * Date: 5/8/13 - * Time: 10:55 AM + * Date: 5/8/13 + * Time: 10:55 AM */ public interface IGVFeatureReader { - public Iterator query(final String chr, final int start, final int end) throws IOException; + Iterator query(final String chr, final int start, final int end) throws IOException; - public Iterator iterator() throws IOException; + Iterator iterator() throws IOException; - public List getSequenceNames(); + List getSequenceNames(); - public Object getHeader(); + Object getHeader(); } diff --git a/src/main/java/org/broad/igv/feature/tribble/MUTCodec.java b/src/main/java/org/broad/igv/feature/tribble/MUTCodec.java index 27396107cb..388ec35999 100644 --- a/src/main/java/org/broad/igv/feature/tribble/MUTCodec.java +++ b/src/main/java/org/broad/igv/feature/tribble/MUTCodec.java @@ -180,7 +180,7 @@ public Mutation decode(String line) { int start; try { - start = Integer.parseInt(tokens[startColumn].trim()); + start = Integer.parseInt(tokens[startColumn].trim()) - 1; } catch (NumberFormatException e) { errorCount++; if (errorCount > 100) { @@ -204,14 +204,6 @@ public Mutation decode(String line) { } } - - // MAF files use the 1-based inclusive convention for coordinates. The convention is not - // specified for MUT files, and it appears both conventions have been used. We can detect - // the convention used for single base mutations by testing start == end. - if (isMAF || (start == end) || coords == TrackProperties.BaseCoord.ONE) { - start--; - } - String sampleId = "Unknown"; if(sampleColumn >= 0) { sampleId = tokens[sampleColumn].trim(); diff --git a/src/main/java/org/broad/igv/feature/tribble/reader/BamIndex.java b/src/main/java/org/broad/igv/feature/tribble/reader/BamIndex.java new file mode 100644 index 0000000000..bf32fbbf5a --- /dev/null +++ b/src/main/java/org/broad/igv/feature/tribble/reader/BamIndex.java @@ -0,0 +1,310 @@ +package org.broad.igv.feature.tribble.reader; + +import htsjdk.samtools.util.BlockCompressedInputStream; +import org.broad.igv.feature.genome.Genome; +import org.broad.igv.util.LittleEndianInputStream; + +import java.io.ByteArrayInputStream; +import java.io.ByteArrayOutputStream; +import java.io.EOFException; +import java.io.IOException; +import java.nio.ByteBuffer; +import java.util.*; +import java.util.zip.GZIPInputStream; + +public class BamIndex { + + // Represents a BAM index. + // Code is based heavily on bam.js, part of the Dalliance Genome Explorer, (c) Thomas Down 2006-2001. + + int BAI_MAGIC = 21578050; + int TABIX_MAGIC = 21578324; + + int MB = 1000000; + String[] seqNames; + Map sequenceIndexMap; + private boolean tabix; + private long firstBlockPosition; + private long lastBlockPosition; + + private Map indeces; + + + public void parse(byte[] arrayBuffer, Genome genome) throws IOException { + + // TODO -- handle big endian? + LittleEndianInputStream lis; + + // Some indexs are bgzipped, specifically tabix, and csi. Bam (bai) are not. Tribble is usually not. + // Check first 2 bytes of file for gzip magic number, and inflate if neccessary + if (arrayBuffer[0] == 31 && arrayBuffer[1] == -117) { // gzipped + lis = new LittleEndianInputStream(new BlockCompressedInputStream(new ByteArrayInputStream(arrayBuffer))); + } else { + lis = new LittleEndianInputStream(new ByteArrayInputStream(arrayBuffer)); + } + + + int magic = lis.readInt(); + Map sequenceIndexMap = new HashMap<>(); + + + if (!(magic == BAI_MAGIC || magic == TABIX_MAGIC)) { + throw new RuntimeException("Unrecognized magic value: " + magic); + } + + long blockMin = Long.MAX_VALUE; + long blockMax = 0l; + String[] seqNames; + + this.tabix = magic == TABIX_MAGIC; + + int nref = lis.readInt(); + seqNames = new String[nref]; + + if (tabix) { + // Tabix header parameters aren't used, but they must be read to advance the pointer + int format = lis.readInt(); + int col_seq = lis.readInt(); + int col_beg = lis.readInt(); + int col_end = lis.readInt(); + int meta = lis.readInt(); + int skip = lis.readInt(); + int l_nm = lis.readInt(); + + for (int i = 0; i < nref; i++) { + + String seq_name = lis.readString(); + // Translate to "official" chr name. + if (genome != null) { + seq_name = genome.getCanonicalChrName(seq_name); + } + sequenceIndexMap.put(seq_name, i); + seqNames[i] = seq_name; + } + } + + this.firstBlockPosition = blockMin; + this.lastBlockPosition = blockMax; + this.seqNames = seqNames; + this.sequenceIndexMap = sequenceIndexMap; + this.indeces = new HashMap<>(); + + // Loop through sequences + for (int ref = 0; ref < nref; ref++) { + + int nbin = lis.readInt(); + Map> binIndex = new HashMap<>(); + List linearIndex = new ArrayList<>(); + + for (int b = 0; b < nbin; b++) { + int binNumber = lis.readInt(); + if (binNumber == 37450) { + int nchnk = lis.readInt(); + // This is a psuedo bin, not used but we have to consume the bytes + VPointer cs = new VPointer(lis.readLong()); // unmapped beg + VPointer ce = new VPointer(lis.readLong()); // unmapped end + long n_maped = lis.readLong(); + long nUnmapped = lis.readLong(); + } else { + + binIndex.put(binNumber, new ArrayList<>()); + int nchnk = lis.readInt(); // # of chunks for this bin + + for (int i = 0; i < nchnk; i++) { + VPointer cs = new VPointer(lis.readLong()); //chunk_beg + VPointer ce = new VPointer(lis.readLong()); //chunk_end + + if (cs.block < blockMin) { + blockMin = cs.block; // Block containing first alignment + } + if (ce.block > blockMax) { + blockMax = ce.block; + } + binIndex.get(binNumber).add(new Chunk(cs, ce)); + + } + } + } + + List binNumberList = new ArrayList<>(binIndex.keySet()); + Collections.sort(binNumberList); + + int nintv = lis.readInt(); + for (int i = 0; i < nintv; i++) { + VPointer cs = new VPointer(lis.readLong()); + linearIndex.add(cs); // Might be null + } + + if (nbin > 0) { + this.indeces.put(ref, new SeqIndex(binIndex, linearIndex)); + } + } + + + this.firstBlockPosition = blockMin; + this.lastBlockPosition = blockMax; + this.sequenceIndexMap = sequenceIndexMap; + this.tabix = tabix; + + } + + + public Set sequenceNames() { + return this.sequenceIndexMap.keySet(); + } + + /** + * Fetch blocks for a particular genomic range. This method is public so it can be unit-tested. + * + * @param refId the sequence dictionary index of the chromosome + * @param min genomic start position + * @param max genomic end position + */ + List chunksForRange(int refId, int min, int max) { + + SeqIndex ba = this.indeces.get(refId); + + if (ba == null) { + return Collections.EMPTY_LIST; + } else { + List overlappingBins = reg2bins(min, max); // List of bin ranges that overlap min, max + List chunks = new ArrayList<>(); + + // Find chunks in overlapping bins. Leaf bins (< 4681) are not pruned + for (int[] binRange : overlappingBins) { + for (int bin = binRange[0]; bin <= binRange[1]; bin++) { + if (ba.binIndex.get(bin) != null) { + List binChunks = ba.binIndex.get(bin); + chunks.addAll(binChunks); + } + } + } + + + // Use the linear index to find minimum file position of chunks that could contain alignments in the region + int nintv = ba.linearIndex.size(); + VPointer lowest = null; + int minLin = Math.min(min >> 14, nintv - 1); + int maxLin = Math.min(max >> 14, nintv - 1); + for (int i = minLin; i <= maxLin; ++i) { + VPointer vp = ba.linearIndex.get(i); + if (vp != null) { + // todo -- I think, but am not sure, that the values in the linear index have to be in increasing order. So the first non-null should be minimum + if (lowest == null || vp.isLessThan(lowest)) { + lowest = vp; + } + } + } + return optimizeChunks(chunks, lowest); + } + } + + + List optimizeChunks(List chunks, VPointer lowest) { + + List mergedChunks = new ArrayList<>(); + Chunk lastChunk = null; + + if (chunks.isEmpty()) return chunks; + + chunks.sort((c0, c1) -> { + long dif = c0.left.block - c1.left.block; + if (dif != 0) { + return (int) dif; + } else { + return c0.left.offset - c1.left.offset; + } + }); + + for (Chunk chunk : chunks) { + + if (lowest == null || chunk.right.isGreaterThan(lowest)) { + if (lastChunk == null) { + mergedChunks.add(chunk); + lastChunk = chunk; + } else { + if (canMerge(lastChunk, chunk)) { + if (chunk.right.isGreaterThan(lastChunk.right)) { + lastChunk.right = chunk.right; + } + } else { + mergedChunks.add(chunk); + lastChunk = chunk; + } + } + } else { + //console.log(`skipping chunk ${chunk.minv.block} - ${chunk.maxv.block}`) + } + } + + return mergedChunks; + } + + // +// /** +// * Merge 2 blocks if the gap between them is < 1kb and the total resulting size < 100mb +// * +// * @param chunk1 +// * @param chunk2 +// * @returns {boolean|boolean} +// */ + boolean canMerge(Chunk chunk1, Chunk chunk2) { + long gap = chunk2.left.block - chunk1.right.block; + long total = chunk2.right.block - chunk1.left.block; + return gap < 30000 && total < 10 * MB; + } + + + /** + * Calculate the list of bins that overlap with region [beg, end] + * + * @return List of int arrays, each defining a bin range + */ + List reg2bins(int beg, int end) { + List list = new ArrayList<>(); + if (end >= 1 << 29) end = 1 << 29; + --end; + list.add(new int[]{0, 0}); + list.add(new int[]{1 + (beg >> 26), 1 + (end >> 26)}); + list.add(new int[]{9 + (beg >> 23), 9 + (end >> 23)}); + list.add(new int[]{73 + (beg >> 20), 73 + (end >> 20)}); + list.add(new int[]{585 + (beg >> 17), 585 + (end >> 17)}); + list.add(new int[]{4681 + (beg >> 14), 4681 + (end >> 14)}); + return list; + } + + static class Block { + Chunk minv; + Chunk maxv; + int bin; + + public Block(Chunk minv, Chunk maxv, int bin) { + this.minv = minv; + this.maxv = maxv; + this.bin = bin; + } + } + + static class Chunk { + + VPointer left; + VPointer right; + + public Chunk(VPointer left, VPointer right) { + this.left = left; + this.right = right; + } + } + + static class SeqIndex { + Map> binIndex; + List linearIndex; + + public SeqIndex(Map> binIndex, List linearIndex) { + this.binIndex = binIndex; + this.linearIndex = linearIndex; + } + } + +} + diff --git a/src/main/java/org/broad/igv/feature/tribble/reader/CSIIndex.java b/src/main/java/org/broad/igv/feature/tribble/reader/CSIIndex.java new file mode 100644 index 0000000000..b1f6b2ffb3 --- /dev/null +++ b/src/main/java/org/broad/igv/feature/tribble/reader/CSIIndex.java @@ -0,0 +1,328 @@ +package org.broad.igv.feature.tribble.reader; + +import htsjdk.samtools.util.BlockCompressedInputStream; +import org.broad.igv.feature.genome.Genome; +import org.broad.igv.util.LittleEndianInputStream; + +import java.io.ByteArrayInputStream; +import java.io.IOException; +import java.util.*; + +public class CSIIndex { + + // Represents a BAM index. + // Code is based heavily on bam.js, part of the Dalliance Genome Explorer, (c) Thomas Down 2006-2001. + + static final int CSI1_MAGIC = 21582659; // CSI\1 + static final int CSI2_MAGIC = 38359875; // CSI\2 + + int MB = 1000000; + String[] seqNames; + Map sequenceIndexMap; + private boolean tabix; + private long firstBlockPosition; + private long lastBlockPosition; + + private Map indeces; + private int minShift; + private int depth; + private int maxBinNumber; + + + public void parse(byte[] arrayBuffer, Genome genome) throws IOException { + + // TODO -- handle big endian? + LittleEndianInputStream lis; + + // Some indexs are bgzipped, specifically tabix, and csi. Bam (bai) are not. Tribble is usually not. + // Check first 2 bytes of file for gzip magic number, and inflate if neccessary + if (arrayBuffer[0] == 31 && arrayBuffer[1] == -117) { // gzipped + lis = new LittleEndianInputStream(new BlockCompressedInputStream(new ByteArrayInputStream(arrayBuffer))); + } else { + lis = new LittleEndianInputStream(new ByteArrayInputStream(arrayBuffer)); + } + + int magic = lis.readInt(); + + if (magic != CSI1_MAGIC) { + if (magic == CSI2_MAGIC) { + throw new RuntimeException("CSI version 2 is not supported. Please enter an issue at https://github.com/igvteam/igv.js"); + } else { + throw new RuntimeException("Not a CSI index"); + } + } + + //const indices = [] + long blockMin = Long.MAX_VALUE; + long blockMax = 0l; + + Map sequenceIndexMap = new HashMap<>(); + + this.minShift = lis.readInt(); + this.depth = lis.readInt(); + int lAux = lis.readInt(); + + this.maxBinNumber = (((1 << (this.depth + 1) * 3) - 1) / 7) + 1; + List seqNames = new ArrayList<>(); + if (lAux >= 28) { + // Tabix header parameters aren't used, but they must be read to advance the pointer + int format = lis.readInt(); + int col_seq = lis.readInt(); + int col_beg = lis.readInt(); + int col_end = lis.readInt(); + int meta = lis.readInt(); + int skip = lis.readInt(); + int l_nm = lis.readInt(); + + int i = 0; + int nameCharCount = 0; + while (nameCharCount < l_nm) { + String seq_name = lis.readString(); + nameCharCount += seq_name.length() + 1; + // Translate to "official" chr name. + if (genome != null) { + seq_name = genome.getCanonicalChrName(seq_name); + } + sequenceIndexMap.put(seq_name, i); + seqNames.add(seq_name); + i++; + } + } + + this.indeces = new HashMap<>(); + + // Loop through sequence + int nref = lis.readInt(); + for (int ref = 0; ref < nref; ref++) { + + int nbin = lis.readInt(); + Map> binIndex = new HashMap<>(); + Map linearOffset = new HashMap<>(); + + for (int b = 0; b < nbin; b++) { + + int binNumber = lis.readInt(); + linearOffset.put(binNumber, new VPointer(lis.readLong())); + + if (binNumber > this.maxBinNumber) { + // This is a psuedo bin, not used but we have to consume the bytes + int nchnk = lis.readInt(); + VPointer cs = new VPointer(lis.readLong()); // unmapped beg + VPointer ce = new VPointer(lis.readLong()); // unmapped end + long n_maped = lis.readLong(); + long nUnmapped = lis.readLong(); + } else { + + binIndex.put(binNumber, new ArrayList<>()); + int nchnk = lis.readInt(); // # of chunks for this bin + + for (int i = 0; i < nchnk; i++) { + VPointer cs = new VPointer(lis.readLong()); //chunk_beg + VPointer ce = new VPointer(lis.readLong()); //chunk_end + + if (cs.block < blockMin) { + blockMin = cs.block; // Block containing first alignment + } + if (ce.block > blockMax) { + blockMax = ce.block; + } + binIndex.get(binNumber).add(new Chunk(cs, ce)); + + } + } + } + + List binNumberList = new ArrayList<>(binIndex.keySet()); + Collections.sort(binNumberList); + + if (nbin > 0) { + this.indeces.put(ref, new SeqIndex(binIndex, linearOffset)); + } + } + + + this.firstBlockPosition = blockMin; + this.lastBlockPosition = blockMax; + this.seqNames = seqNames.toArray(new String[seqNames.size()]); + this.sequenceIndexMap = sequenceIndexMap; + + } + + + public Set sequenceNames() { + return this.sequenceIndexMap.keySet(); + } + + /** + * Fetch blocks for a particular genomic range. This method is public so it can be unit-tested. + * + * @param refId the sequence dictionary index of the chromosome + * @param min genomic start position + * @param max genomic end position + */ + List chunksForRange(int refId, int min, int max) { + + SeqIndex ba = this.indeces.get(refId); + + if (ba == null) { + return Collections.EMPTY_LIST; + } else { + List overlappingBins = reg2bins(min, max); // List of bin ranges that overlap min, max + if (overlappingBins.size() == 0) return Collections.EMPTY_LIST; + + List chunks = new ArrayList<>(); + + // Find chunks in overlapping bins. Leaf bins (< 4681) are not pruned + for (int[] binRange : overlappingBins) { + for (int bin = binRange[0]; bin <= binRange[1]; bin++) { + if (ba.binIndex.get(bin) != null) { + List binChunks = ba.binIndex.get(bin); + chunks.addAll(binChunks); + } + } + } + + + // Use the linear index to find minimum file position of chunks that could contain alignments in the region + + VPointer lowest = ba.linearOffsets.get(overlappingBins.get(0)); + + return optimizeChunks(chunks, lowest); + } + } + + + List optimizeChunks(List chunks, VPointer lowest) { + + List mergedChunks = new ArrayList<>(); + Chunk lastChunk = null; + + if (chunks.isEmpty()) return chunks; + + chunks.sort((c0, c1) -> { + long dif = c0.left.block - c1.left.block; + if (dif != 0) { + return (int) dif; + } else { + return c0.left.offset - c1.left.offset; + } + }); + + for (Chunk chunk : chunks) { + + if (lowest == null || chunk.right.isGreaterThan(lowest)) { + if (lastChunk == null) { + mergedChunks.add(chunk); + lastChunk = chunk; + } else { + if (canMerge(lastChunk, chunk)) { + if (chunk.right.isGreaterThan(lastChunk.right)) { + lastChunk.right = chunk.right; + } + } else { + mergedChunks.add(chunk); + lastChunk = chunk; + } + } + } else { + //console.log(`skipping chunk ${chunk.minv.block} - ${chunk.maxv.block}`) + } + } + + return mergedChunks; + } + + // +// /** +// * Merge 2 blocks if the gap between them is < 1kb and the total resulting size < 100mb +// * +// * @param chunk1 +// * @param chunk2 +// * @returns {boolean|boolean} +// */ + boolean canMerge(Chunk chunk1, Chunk chunk2) { + long gap = chunk2.left.block - chunk1.right.block; + long total = chunk2.right.block - chunk1.left.block; + return gap < 30000 && total < 10 * MB; + } + + + /** + * Calculate the list of bins that overlap with region [beg, end] + * reg2bins implementation adapted from GMOD/tabix-js https://github.com/GMOD/tabix-js/blob/master/src/csi.ts + * + * @return List of int arrays, each defining a bin range + */ + List reg2bins(int beg, int end) { + + beg -= 1; // < convert to 1-based closed + if (beg < 1) beg = 1; + if (end > Math.pow(2, 34)) end = (int) Math.pow(2, 34) ;// 17 GiB ought to be enough for anybody + end -= 1; + int l = 0; + int t = 0; + int s = this.minShift + this.depth * 3; + + + List bins = new ArrayList<>(); + for (; l <= this.depth; s -= 3, t += (1 << l * 3), l += 1) { + int b = t + (beg >> s); + int e = t + (end >> s); + if (e - b + bins.size() > this.maxBinNumber) + throw new RuntimeException("" + (beg - end) + "is too large for current binning scheme (shift " + + this.minShift + ", depth " + this.depth + "), try a smaller query or a coarser index binning scheme"); + //for (let i = b; i <= e; i += 1) bins.push(i) + bins.add(new int[]{b, e}); + } + + return bins; + } + + public int getDepth() { + return this.depth; + } + + public int getMinShift() { + return this.minShift; + } + + public int getMaxBinNumber() { + return this.maxBinNumber; + } + + + static class Block { + Chunk minv; + Chunk maxv; + int bin; + + public Block(Chunk minv, Chunk maxv, int bin) { + this.minv = minv; + this.maxv = maxv; + this.bin = bin; + } + } + + static class Chunk { + + VPointer left; + VPointer right; + + public Chunk(VPointer left, VPointer right) { + this.left = left; + this.right = right; + } + } + + static class SeqIndex { + Map> binIndex; + Map linearOffsets; + + public SeqIndex(Map> binIndex, Map linearOffsets) { + this.binIndex = binIndex; + this.linearOffsets = linearOffsets; + } + } + +} + diff --git a/src/main/java/org/broad/igv/feature/tribble/reader/VPointer.java b/src/main/java/org/broad/igv/feature/tribble/reader/VPointer.java new file mode 100644 index 0000000000..ea87ee222d --- /dev/null +++ b/src/main/java/org/broad/igv/feature/tribble/reader/VPointer.java @@ -0,0 +1,29 @@ +package org.broad.igv.feature.tribble.reader; + + +public class VPointer { + + private static final int SHIFT_AMOUNT = 16; + private static final int OFFSET_MASK = 0xffff; + private static final long ADDRESS_MASK = 0xFFFFFFFFFFFFL; + + + long block; + int offset; + + public VPointer(long v) { + this.block = (v >> SHIFT_AMOUNT) & ADDRESS_MASK; + this.offset = (int) (v & OFFSET_MASK); + } + + public boolean isLessThan(VPointer vp) { + return this.block < vp.block || + (this.block == vp.block && this.offset < vp.offset); + } + + public boolean isGreaterThan(VPointer vp) { + return this.block > vp.block || + (this.block == vp.block && this.offset > vp.offset); + } + +} \ No newline at end of file diff --git a/src/main/java/org/broad/igv/htsget/HtsgetVariantSource.java b/src/main/java/org/broad/igv/htsget/HtsgetVariantSource.java index 5305fd77ee..9d4f6170cd 100644 --- a/src/main/java/org/broad/igv/htsget/HtsgetVariantSource.java +++ b/src/main/java/org/broad/igv/htsget/HtsgetVariantSource.java @@ -92,7 +92,7 @@ public List getCoverageScores(String chr, int start, int end, int zo } @Override - public int getFeatureWindowSize() { + public int estimateFeatureWindowSize() { return featureWindowSize; } diff --git a/src/main/java/org/broad/igv/session/IGVSessionReader.java b/src/main/java/org/broad/igv/session/IGVSessionReader.java index bc450e19c3..6f43ea8dbd 100644 --- a/src/main/java/org/broad/igv/session/IGVSessionReader.java +++ b/src/main/java/org/broad/igv/session/IGVSessionReader.java @@ -252,9 +252,11 @@ private void processRootNode(Session session, Node node, HashMap additionalInfor try { GenomeListItem item = GenomeListManager.getInstance().getGenomeListItem(genomeId); if (item != null) { + // Genome specified by know ID (e.g. hg19) genomePath = item.getPath(); GenomeManager.getInstance().loadGenome(item.getPath(), null); } else { + // Not a recognized ID, possible a local path or URL genomePath = genomeId; if (!ParsingUtils.fileExists(genomePath)) { genomePath = getAbsolutePath(genomeId, rootPath); diff --git a/src/main/java/org/broad/igv/tools/motiffinder/MotifFinderPlugin.java b/src/main/java/org/broad/igv/tools/motiffinder/MotifFinderPlugin.java index ee5ddc0bb5..431ec107f5 100644 --- a/src/main/java/org/broad/igv/tools/motiffinder/MotifFinderPlugin.java +++ b/src/main/java/org/broad/igv/tools/motiffinder/MotifFinderPlugin.java @@ -25,10 +25,7 @@ package org.broad.igv.tools.motiffinder; -import org.broad.igv.feature.CachingFeatureSource; import org.broad.igv.feature.Strand; -import org.broad.igv.feature.genome.GenomeManager; -import org.broad.igv.track.FeatureTrack; import org.broad.igv.track.MotifTrack; import org.broad.igv.track.Track; import org.broad.igv.ui.IGV; @@ -37,8 +34,6 @@ import javax.swing.*; import java.awt.*; -import java.awt.event.ActionEvent; -import java.awt.event.ActionListener; import java.util.ArrayList; import java.util.List; diff --git a/src/main/java/org/broad/igv/tools/motiffinder/MotifFinderSource.java b/src/main/java/org/broad/igv/tools/motiffinder/MotifFinderSource.java index 382d7b63ba..2c1724afe6 100644 --- a/src/main/java/org/broad/igv/tools/motiffinder/MotifFinderSource.java +++ b/src/main/java/org/broad/igv/tools/motiffinder/MotifFinderSource.java @@ -71,6 +71,9 @@ public class MotifFinderSource implements FeatureSource, Persistable { private Strand strand; + /** + * Empty constructor for unmarshalling session + */ public MotifFinderSource() { this.genome = GenomeManager.getInstance().getCurrentGenome(); } @@ -167,7 +170,7 @@ public List getCoverageScores(String chr, int start, int end, int zo } @Override - public int getFeatureWindowSize() { + public int estimateFeatureWindowSize() { return this.featureWindowSize; } diff --git a/src/main/java/org/broad/igv/track/FeatureCollectionSource.java b/src/main/java/org/broad/igv/track/FeatureCollectionSource.java index be7674e720..7a7612f5d8 100644 --- a/src/main/java/org/broad/igv/track/FeatureCollectionSource.java +++ b/src/main/java/org/broad/igv/track/FeatureCollectionSource.java @@ -38,6 +38,7 @@ import org.broad.igv.util.collections.CollUtils; import htsjdk.tribble.Feature; +import java.io.IOException; import java.util.*; /** @@ -65,7 +66,6 @@ public FeatureCollectionSource(Iterable allFeatures, Genome g initFeatures(allFeatures); coverageData = new CoverageDataSource(genome); coverageData.computeGenomeCoverage(); - sampleGenomeFeatures(); } public List getCoverageScores(String chr, int startLocation, int endLocation, int zoom) { @@ -81,26 +81,22 @@ public List getCoverageScores(String chr, int startLocation, int end * @return */ public Iterator getFeatures(String chr, int start, int end) { - return getFeatureList(chr, start, end).iterator(); - } - - public List getFeatureList(String chr, int start, int end) { List features = featureMap.get(chr); if (features == null) { - return Collections.emptyList(); + return Collections.emptyList().iterator(); } List filteredFeatures = CollUtils.filter(features, FeatureUtils.getOverlapPredicate(chr, start, end)); - return filteredFeatures; + return filteredFeatures.iterator(); } @Override - public boolean isLoaded(ReferenceFrame frame) { - return true; // All features are loaded by definition - } - - public List getFeatures(String chr) { - return featureMap.get(chr); + public List getAllFeatures() throws IOException { + List allFeatures = new ArrayList<>(); + for(List f : featureMap.values()) { + allFeatures.addAll(f); + } + return allFeatures; } private void initFeatures(Iterable allFeatures) { @@ -119,10 +115,6 @@ private void initFeatures(Iterable allFeatures) { for (List featureList : featureMap.values()) { FeatureUtils.sortFeatureList(featureList); } - - if (featureMap.size() < 100) { - sampleGenomeFeatures(); - } } @@ -156,7 +148,7 @@ protected void sampleGenomeFeatures() { int sampleLength = (int) ((double) genome.getNominalLength() / (1000 * 700)); int lastFeaturePosition = -1; for (String chr : genome.getLongChromosomeNames()) { - List features = getFeatures(chr); + List features =featureMap.get(chr); if (features != null) { long offset = genome.getCumulativeOffset(chr); for (Feature feature : features) { diff --git a/src/main/java/org/broad/igv/track/FeatureSource.java b/src/main/java/org/broad/igv/track/FeatureSource.java index b001557f65..f7af7ce622 100644 --- a/src/main/java/org/broad/igv/track/FeatureSource.java +++ b/src/main/java/org/broad/igv/track/FeatureSource.java @@ -27,7 +27,6 @@ import htsjdk.tribble.Feature; import org.broad.igv.feature.LocusScore; -import org.broad.igv.ui.panel.ReferenceFrame; import java.io.IOException; import java.util.Iterator; @@ -41,10 +40,6 @@ */ public interface FeatureSource { - default boolean isLoaded(ReferenceFrame frame) { - return false; - } - /** * Return an iterator over all features that overlap the interval. The coordinates are in the "UCSC" convention, * that is first base is zero and the interval is not inclusive of the end position, i.e. an interval @@ -58,6 +53,18 @@ default boolean isLoaded(ReferenceFrame frame) { Iterator getFeatures(String chr, int start, int end) throws IOException; + /** + * Return all features for the entire genome as a list. Not supported by all feature sources. + * + * @return + * @throws IOException + */ + default List getAllFeatures() throws IOException { + return null; + } + + + /** * Return a list of coverage values spanning the given interval. This can be null if coverage is not known * or relevant. @@ -76,12 +83,9 @@ default boolean isLoaded(ReferenceFrame frame) { * rendering features. When zoomed out beyond this interval coverage is shown, if available, or a message to * zoom in. * - * TODO: It seems slightly odd to have this property here, but on the other hand FeatureSource objects have - * TODO: access to the information required to estimate this value. Still, consider removing this from the interface. - * * @return the feature window threshold in base-pairs */ - default int getFeatureWindowSize() { + default int estimateFeatureWindowSize() { return -1; } diff --git a/src/main/java/org/broad/igv/track/FeatureTrack.java b/src/main/java/org/broad/igv/track/FeatureTrack.java index 35865fbfa8..d51da2788e 100644 --- a/src/main/java/org/broad/igv/track/FeatureTrack.java +++ b/src/main/java/org/broad/igv/track/FeatureTrack.java @@ -25,8 +25,11 @@ package org.broad.igv.track; +import htsjdk.samtools.util.Locatable; import htsjdk.tribble.Feature; import htsjdk.tribble.TribbleException; +import org.broad.igv.feature.genome.Genome; +import org.broad.igv.feature.genome.GenomeManager; import org.broad.igv.logging.*; import org.broad.igv.Globals; import org.broad.igv.event.DataLoadedEvent; @@ -34,8 +37,6 @@ import org.broad.igv.event.IGVEventObserver; import org.broad.igv.feature.*; import org.broad.igv.feature.Range; -import org.broad.igv.feature.genome.Genome; -import org.broad.igv.feature.genome.GenomeManager; import org.broad.igv.prefs.Constants; import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.renderer.*; @@ -118,6 +119,7 @@ public class FeatureTrack extends AbstractTrack implements IGVEventObserver { private boolean groupByStrand = false; private String labelField; + private ArrayList wgFeatures; public FeatureTrack() { @@ -195,7 +197,7 @@ protected void init(ResourceLocator locator, FeatureSource source) { if (vizWindow != null) { visibilityWindow = vizWindow; } else { - int sourceFeatureWindowSize = source.getFeatureWindowSize(); + int sourceFeatureWindowSize = source.estimateFeatureWindowSize(); int defVisibilityWindow = PreferencesManager.getPreferences().getAsInt(Constants.DEFAULT_VISIBILITY_WINDOW); if (sourceFeatureWindowSize > 0 && defVisibilityWindow > 0) { // Only apply a default if the feature source supports visibility window. visibilityWindow = defVisibilityWindow * 1000; @@ -679,69 +681,58 @@ public boolean isReadyToPaint(ReferenceFrame frame) { } } - public void load(ReferenceFrame frame) { - loadFeatures(frame.getChrName(), (int) frame.getOrigin(), (int) frame.getEnd(), frame.getName()); - } - /** - * Loads and segregates features into rows such that they do not overlap. - * - * @param chr - * @param start - * @param end + * Loads and segregates features for the reference frame into rows such that they do not overlap. */ - protected void loadFeatures(final String chr, final int start, final int end, final String frame) { + public void load(ReferenceFrame frame) { if (source == null) { return; } + final String chr = frame.getChrName(); + final int start = (int) frame.getOrigin(); + final int end = (int) frame.getEnd(); + try { + Iterator iter; int expandedStart; int expandedEnd; - int vw = getVisibilityWindow(); - if (vw > 0) { - int delta = (end - start) / 2; - expandedStart = start - delta; - expandedEnd = end + delta; - if (expandedEnd < 0) { - expandedEnd = Integer.MAX_VALUE; // overflow - } - } else { - expandedStart = 0; - expandedEnd = Integer.MAX_VALUE; - } - - - //Make sure we are only querying within the chromosome we allow for somewhat pathological cases of start - //being negative and end being outside, but only if directly queried. Our expansion should not - //set start < 0 or end > chromosomeLength - if (start >= 0) { - expandedStart = Math.max(0, expandedStart); - } - Genome genome = GenomeManager.getInstance().getCurrentGenome(); - if (genome != null) { - Chromosome c = genome.getChromosome(chr); - if (c != null && end < c.getLength()) expandedEnd = Math.min(c.getLength(), expandedEnd); + if(Globals.CHR_ALL.equals(chr)) { + iter = sampleGenomeFeatures().iterator(); + expandedStart = 0; + expandedEnd = Integer.MAX_VALUE; + } else { + int vw = getVisibilityWindow(); + if (vw > 0) { + int delta = (end - start) / 2; + expandedStart = start < 0 ? start : start - delta; + expandedEnd = end + delta; + if (expandedEnd < 0) { + expandedEnd = Integer.MAX_VALUE; // assumed integer overflow + } + } else { + expandedStart = 0; + expandedEnd = Integer.MAX_VALUE; + } + iter = source.getFeatures(chr, expandedStart, expandedEnd); } - Iterator iter = source.getFeatures(chr, expandedStart, expandedEnd); - if (iter == null) { PackedFeatures pf = new PackedFeatures(chr, expandedStart, expandedEnd); - packedFeaturesMap.put(frame, pf); + packedFeaturesMap.put(frame.getName(), pf); } else { PackedFeatures pf = new PackedFeatures(chr, expandedStart, expandedEnd, iter, this.getDisplayMode(), groupByStrand); - packedFeaturesMap.put(frame, pf); + packedFeaturesMap.put(frame.getName(), pf); //log.warn("Loaded " + chr + " " + expandedStart + "-" + expandedEnd); } } catch (Exception e) { // Mark the interval with an empty feature list to prevent an endless loop of load attempts. PackedFeatures pf = new PackedFeatures(chr, start, end); - packedFeaturesMap.put(frame, pf); + packedFeaturesMap.put(frame.getName(), pf); String msg = "Error loading features for interval: " + chr + ":" + start + "-" + end + "
" + e.toString(); MessageUtils.showMessage(msg); log.error(msg, e); @@ -755,7 +746,6 @@ public void render(RenderContext context, Rectangle rect) { renderRect.y = renderRect.y + margin; renderRect.height -= margin; - showFeatures = isShowFeatures(context.getReferenceFrame()); if (showFeatures) { if (lastFeatureMode != null) { @@ -785,13 +775,13 @@ public void render(RenderContext context, Rectangle rect) { protected boolean isShowFeatures(ReferenceFrame frame) { - if (frame.getChrName().equals(Globals.CHR_ALL)) { - return false; - } else { - double windowSize = frame.getEnd() - frame.getOrigin(); - int vw = getVisibilityWindow(); - return (vw <= 0 || windowSize <= vw); - } +// if (frame.getChrName().equals(Globals.CHR_ALL) && coverageRenderer != null) { +// return false; +// } else { + double windowSize = frame.getEnd() - frame.getOrigin(); + int vw = getVisibilityWindow(); + return (vw <= 0 || windowSize <= vw); +// } } protected void renderCoverage(RenderContext context, Rectangle inputRect) { @@ -1024,6 +1014,40 @@ public List getVisibleFeatures(ReferenceFrame frame) { } } + private List sampleGenomeFeatures() throws IOException { + + if (wgFeatures == null) { + + Genome genome = GenomeManager.getInstance().getCurrentGenome(); + List allFeatures = this.source.getAllFeatures(); + if (allFeatures == null) return Collections.EMPTY_LIST; + + wgFeatures = new ArrayList(allFeatures.size()); + + wgFeatures.sort(Comparator.comparingInt(Locatable::getStart)); + + Set chrNames = new HashSet<>(genome.getLongChromosomeNames()); + + for (Feature feature : allFeatures) { + + String chr = genome.getCanonicalChrName(feature.getChr()); + if (!chrNames.contains(chr)) continue; + + long offset = genome.getCumulativeOffset(chr); + + if (feature instanceof IGVFeature) { + IGVFeature f = (IGVFeature) feature; + int genStart = (int) ((offset + f.getStart()) / 1000); + int genEnd = (int) ((offset + f.getEnd()) / 1000); + + WGFeature f2 = new WGFeature(f, genStart, genEnd); + + wgFeatures.add(f2); + } + } + } + return wgFeatures; + } public void setTrackLine(String trackLine) { this.trackLine = trackLine; diff --git a/src/main/java/org/broad/igv/track/MotifTrack.java b/src/main/java/org/broad/igv/track/MotifTrack.java index e8d2724354..dc60bfc335 100644 --- a/src/main/java/org/broad/igv/track/MotifTrack.java +++ b/src/main/java/org/broad/igv/track/MotifTrack.java @@ -1,6 +1,5 @@ package org.broad.igv.track; -import org.broad.igv.feature.CachingFeatureSource; import org.broad.igv.feature.Strand; import org.broad.igv.feature.genome.GenomeManager; import org.broad.igv.tools.motiffinder.MotifFinderSource; @@ -26,10 +25,7 @@ public MotifTrack(String name, String pattern, Strand strand) { } private void init() { - - MotifFinderSource src = new MotifFinderSource(pattern, strand, GenomeManager.getInstance().getCurrentGenome()); - CachingFeatureSource source = new CachingFeatureSource(src); - super.init(null, source); + super.init(null, new MotifFinderSource(pattern, strand, GenomeManager.getInstance().getCurrentGenome())); setSortable(false); } diff --git a/src/main/java/org/broad/igv/track/MutationTrack.java b/src/main/java/org/broad/igv/track/MutationTrack.java index 7c1d971e42..45c9e9dd36 100644 --- a/src/main/java/org/broad/igv/track/MutationTrack.java +++ b/src/main/java/org/broad/igv/track/MutationTrack.java @@ -71,19 +71,6 @@ public void overlay(RenderContext context, Rectangle rect) { } - /** - * Return a string for popup text. - * - * @param chr - * @param position in genomic coordinates - * @param mouseX - * @return - */ - @Override - public String getValueStringAt(String chr, double position, int mouseX, int mouseY, ReferenceFrame frame) { - return super.getValueStringAt(chr, position, mouseX, mouseY, frame); //To change body of overridden methods use File | Settings | File Templates. - } - @Override public boolean handleDataClick(TrackClickEvent te) { super.openTooltipWindow(te); @@ -91,69 +78,4 @@ public boolean handleDataClick(TrackClickEvent te) { } - private String getOMAText(Mutation mut, String url) { - try { - String omaWebService = url + "&frm=txt&fts=all"; - - String result = HttpUtils.getInstance().getContentsAsString(HttpUtils.createURL(omaWebService)); - - BufferedReader br = new BufferedReader(new StringReader(result)); - String[] headers = br.readLine().split("\t"); - String[] values = br.readLine().split("\t"); - - StringBuffer buf = new StringBuffer(); - - buf.append(""); - buf.append(""); - buf.append(""); - - int n = Math.min(headers.length, values.length); - for (int i = 0; i < n; i++) { - final String header = headers[i].trim(); - final String value = values[i].trim(); - - if (header.length() == 0 || value.length() == 0) continue; - - buf.append(""); - buf.append(""); - } - buf.append("
Data fromMutation Assessor
Type" + mut.getMutationType() + "
"); - buf.append(header); - buf.append(""); - - if (header.equals("MSA") || header.equals("PDB")) { - buf.append(""); - buf.append(header); - buf.append(""); - } else if (header.equals("Uniprot")) { - buf.append(""); - buf.append(value); - buf.append(""); - } else if (header.equals("Refseq")) { - buf.append(""); - buf.append(value); - buf.append(""); - } else if (header.toLowerCase().contains("impact")) { - if (value.toLowerCase().equals("high") || value.toLowerCase().equals("medium")) { - String color = value.toLowerCase().equals("high") ? "red" : "#0033FF"; - buf.append("
"); - buf.append(value); - buf.append("
"); - } else { - buf.append(value); - } - } else { - buf.append(value); - } - buf.append("

"); - - return buf.toString(); - } catch (IOException e) { - log.error("Error accessing OMA ", e); - return null; - } - } - } diff --git a/src/main/java/org/broad/igv/track/TribbleFeatureSource.java b/src/main/java/org/broad/igv/track/TribbleFeatureSource.java index f56891f9b4..f05872505a 100644 --- a/src/main/java/org/broad/igv/track/TribbleFeatureSource.java +++ b/src/main/java/org/broad/igv/track/TribbleFeatureSource.java @@ -118,6 +118,7 @@ public static TribbleFeatureSource getFeatureSource(ResourceLocator locator, Gen } } + indexExists = false; String path = locator.getPath(); if (FileUtils.isRemote(path)) { path = HttpUtils.createURL(path).toExternalForm(); @@ -186,7 +187,7 @@ public Class getFeatureClass() { return featureClass; } - public int getFeatureWindowSize() { + public int estimateFeatureWindowSize() { if (featureWindowSize == null) { this.featureWindowSize = estimateFeatureWindowSize(this.wrappedReader); } @@ -232,6 +233,17 @@ public Iterator getFeatures(String chr, int start, int end) throws IOEx return reader.query(seqName, start, end); } + public List getAllFeatures() throws IOException { + + List allFeatures = new ArrayList<>(); + Iterator iter = reader.iterator(); + while(iter.hasNext()) { + allFeatures.add(iter.next()); + } + return allFeatures; + } + + /** * Return coverage values overlapping the query interval. At this time Tribble sources do not provide * coverage values @@ -389,11 +401,6 @@ public boolean isIndexed() { return false; } - @Override - public boolean isLoaded(ReferenceFrame frame) { - return true; - } - @Override public List getCoverageScores(String chr, int start, int end, int zoom) { return coverageData == null ? Collections.emptyList() : diff --git a/src/main/java/org/broad/igv/track/TribbleListFeatureSource.java b/src/main/java/org/broad/igv/track/TribbleListFeatureSource.java index 20d2816c54..d14e403e9b 100644 --- a/src/main/java/org/broad/igv/track/TribbleListFeatureSource.java +++ b/src/main/java/org/broad/igv/track/TribbleListFeatureSource.java @@ -138,7 +138,7 @@ public List getCoverageScores(String chr, int start, int end, int zo } @Override - public int getFeatureWindowSize() { + public int estimateFeatureWindowSize() { return windowSize; } diff --git a/src/main/java/org/broad/igv/variant/New/Variant.java b/src/main/java/org/broad/igv/variant/New/Variant.java index 06009b28fb..cb70e72cf6 100644 --- a/src/main/java/org/broad/igv/variant/New/Variant.java +++ b/src/main/java/org/broad/igv/variant/New/Variant.java @@ -28,12 +28,15 @@ import htsjdk.tribble.Feature; import org.broad.igv.Globals; +import org.broad.igv.feature.IGVFeature; + +import java.awt.*; /** * Parser for VCF files. */ -public class Variant implements Feature { +public class Variant implements IGVFeature { String chr; int pos; @@ -112,4 +115,18 @@ public String getValueString(int genomicPosition) { } + @Override + public Color getColor() { + return null; + } + + @Override + public float getScore() { + return 0; + } + + @Override + public String getName() { + return null; + } } diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index 68f316b430..05de4685ae 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -295,6 +295,7 @@ SMRT_KINETICS.SHOW_OPTIONS FALSE SCORE_VARIANTS FALSE SAM.AUTOSORT FALSE +SAM.INVERT_SORT FALSE SAM.SHADE_CENTER TRUE SAM.SHOW_REF_SEQ FALSE @@ -314,6 +315,7 @@ SAM.GROUP_ALL FALSE SAM.INVERT_SORT FALSE + SEARCH_ZOOM TRUE SAVE_GOOGLE_CREDENTIALS TRUE MAX_SEQUENCE_RESOLUTION 2 diff --git a/src/test/java/org/broad/igv/feature/tribble/reader/BamIndexTest.java b/src/test/java/org/broad/igv/feature/tribble/reader/BamIndexTest.java new file mode 100644 index 0000000000..c20259abd2 --- /dev/null +++ b/src/test/java/org/broad/igv/feature/tribble/reader/BamIndexTest.java @@ -0,0 +1,171 @@ +/* + * The MIT License (MIT) + * + * Copyright (c) 2007-2015 Broad Institute + * + * Permission is hereby granted, free of charge, to any person obtaining a copy + * of this software and associated documentation files (the "Software"), to deal + * in the Software without restriction, including without limitation the rights + * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell + * copies of the Software, and to permit persons to whom the Software is + * furnished to do so, subject to the following conditions: + * + * The above copyright notice and this permission notice shall be included in + * all copies or substantial portions of the Software. + * + * + * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR + * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, + * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE + * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER + * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, + * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN + * THE SOFTWARE. + */ + +package org.broad.igv.feature.tribble.reader; + +import org.broad.igv.util.TestUtils; +import org.junit.Test; + +import java.io.FileInputStream; +import java.util.List; + +import static org.junit.Assert.*; + +/** + * @author jrobinso + * Date: 1/23/14 + * Time: 4:24 PM + */ +public class BamIndexTest { + + @Test + public void testParse() throws Exception { + + String path = TestUtils.DATA_DIR + "tabix/refGene.hg19.bed.gz.tbi"; + + FileInputStream fis = new FileInputStream(path); + byte[] bytes = fis.readAllBytes(); + + BamIndex tabixIndex = new BamIndex(); + tabixIndex.parse(bytes, null); + assertNotNull(tabixIndex.sequenceIndexMap); + + + } + + @Test + public void testTBIIndex() throws Exception { + + String path = TestUtils.DATA_DIR + "tabix/refGene.hg19.bed.gz.tbi"; + int refID = 26; + int beg = 55369194; + int end = 55369443; + + FileInputStream fis = new FileInputStream(path); + byte[] bytes = fis.readAllBytes(); + + BamIndex tbiIndex = new BamIndex(); + tbiIndex.parse(bytes, null); + + List chunks = tbiIndex.chunksForRange(refID, beg, end); + assertNotNull(tbiIndex.sequenceIndexMap); + assertEquals(chunks.size(), 1); + assertEquals(1640062, chunks.get(0).left.block); + + } + + @Test + public void testCSIIndex() throws Exception { + + String path = TestUtils.DATA_DIR + "tabix/csi-test.vcf.gz.csi"; + int refID = 0; + int beg = 1226000; + int end = 1227000; + + FileInputStream fis = new FileInputStream(path); + byte[] bytes = fis.readAllBytes(); + + CSIIndex csiIndex = new CSIIndex(); + csiIndex.parse(bytes, null); + + List chunks = csiIndex.chunksForRange(refID, beg, end); + assertTrue(chunks.size() > 0); + + } + + @Test + public void testCSIQuery() throws Exception { + + String path = TestUtils.DATA_DIR + "tabix/csi-test.vcf.gz.csi"; + int refID = 0; + int beg = 1226000; + int end = 1227000; + + FileInputStream fis = new FileInputStream(path); + byte[] bytes = fis.readAllBytes(); + + CSIIndex csiIndex = new CSIIndex(); + csiIndex.parse(bytes, null); + + List chunks = csiIndex.chunksForRange(refID, beg, end); + assertEquals(chunks.size(), 1); + assertEquals(74500, chunks.get(0).left.block); + assertEquals(61246, chunks.get(0).left.offset); + assertEquals(94804, chunks.get(0).right.block); + } + + + +// +// test("CSI query - vcf", async function () { +// +// const chr = "chr1", +// beg = 1226000, +// end = 1227000; +// +// const reader = new FeatureFileReader({ +// format: "vcf", +// url: require.resolve("./data/tabix/csi-test.vcf.gz"), +// indexURL: require.resolve("./data/tabix/csi-test.vcf.gz.csi") +// }); +// await reader.readHeader(); +// const features = await reader.readFeatures(chr, beg, end); +// assert.ok(features); +// +// // Verify features are in query range. The features immediately before and after query range are returned by design +// const len = features.length; +// assert.ok(len > 0); +// for (let i = 1; i < len - 1; i++) { +// const f = features[i]; +// assert.ok(f.chr === chr && f.end >= beg && f.start <= end); +// } +// }) +// +// test("CSI query - gtf", async function () { +// +// this.timeout(200000); +// +// const chr = "10", +// beg = 400000, +// end = 500000; +// +// const reader = new FeatureFileReader({ +// format: "gff3", +// url: "https://s3.amazonaws.com/igv.org.genomes/hg38/Homo_sapiens.GRCh38.94.chr.gff3.gz", +// indexURL: "https://s3.amazonaws.com/igv.org.genomes/hg38/Homo_sapiens.GRCh38.94.chr.gff3.gz.csi" +// }); +// await reader.readHeader(); +// const features = await reader.readFeatures(chr, beg, end); +// assert.ok(features); +// +// const len = features.length; +// assert.ok(len > 0); +// for (let i = 1; i < len - 1; i++) { +// const f = features[i]; +// assert.ok(f.chr === chr); +// } +// }) + +} diff --git a/src/test/java/org/broad/igv/feature/tribble/reader/CSIIndexTest.java b/src/test/java/org/broad/igv/feature/tribble/reader/CSIIndexTest.java new file mode 100644 index 0000000000..273cb218dc --- /dev/null +++ b/src/test/java/org/broad/igv/feature/tribble/reader/CSIIndexTest.java @@ -0,0 +1,94 @@ +/* + * The MIT License (MIT) + * + * Copyright (c) 2007-2015 Broad Institute + * + * Permission is hereby granted, free of charge, to any person obtaining a copy + * of this software and associated documentation files (the "Software"), to deal + * in the Software without restriction, including without limitation the rights + * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell + * copies of the Software, and to permit persons to whom the Software is + * furnished to do so, subject to the following conditions: + * + * The above copyright notice and this permission notice shall be included in + * all copies or substantial portions of the Software. + * + * + * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR + * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, + * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE + * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER + * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, + * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN + * THE SOFTWARE. + */ + +package org.broad.igv.feature.tribble.reader; + +import org.broad.igv.util.TestUtils; +import org.junit.Before; +import org.junit.Test; + +import java.io.FileInputStream; +import java.io.IOException; + +import static org.junit.Assert.*; + +/** + * @author jrobinso + * Date: 1/23/14 + * Time: 4:24 PM + */ +public class CSIIndexTest { + + CSIIndex csi; + CSIIndex ucsi; + + @Before + public void init() throws IOException { + + + FileInputStream fis = new FileInputStream(TestUtils.DATA_DIR + "tabix/index_test.bam.csi"); + byte[] bytes = fis.readAllBytes(); + csi = new CSIIndex(); + csi.parse(bytes, null); + + + fis = new FileInputStream(TestUtils.DATA_DIR + "tabix/uncompressed_index.bam.csi"); + bytes = fis.readAllBytes(); + ucsi = new CSIIndex(); + ucsi.parse(bytes, null); + + +// bai = new DiskBasedBAMFileIndex(new File(TEST_DATA_DIR, "index_test.bam.bai"), null); +// csi = new htsjdk.samtools.CSIIndex(new File(TEST_DATA_DIR, "index_test.bam.csi"), false, null); +// mcsi = new htsjdk.samtools.CSIIndex(new File(TEST_DATA_DIR, "index_test.bam.csi"), true, null); +// try { +// ucsi = new CSIIndex(Paths.get(TEST_DATA_DIR, "uncompressed_index.bam.csi"), null); +// } catch (IOException e) { +// e.printStackTrace(); +// } +// ubai = new DiskBasedBAMFileIndex(new File(TEST_DATA_DIR, "uncompressed_index.bam.bai"), null); + } + + @Test + public void testGetNumIndexLevels() { + assertEquals(csi.getDepth(), 5); + assertEquals(ucsi.getDepth(), 6); + } + + @Test + public void testGetMinShift() { + assertEquals(csi.getMinShift(), 14); + assertEquals(ucsi.getMinShift(), 12); + } + + @Test + public void testGetMaxBins() { + assertEquals(csi.getMaxBinNumber(), 37450); + assertEquals(ucsi.getMaxBinNumber(), 299594); + } + + + +} diff --git a/test/data/tabix/TSVC.vcf.gz b/test/data/tabix/TSVC.vcf.gz new file mode 100755 index 0000000000000000000000000000000000000000..10646d699313d9872785c66822db1286838919b8 GIT binary patch literal 4833 zcmV<75+3aziwFb&00000{{{d;LjnNA61`e&bJ|Fd{>=UgRrX7A2lBjYBu-mJjB$MD z*bXMyY+YSZGL~&BfLKW+@&5Yjo{@x*aUhPp72D`#UV7$v`swZ=zyE$Soy9jvI*;=3 z)8XK&aOrQq|2{Z59UTqB|2pZ1XXl5#)6;f8&L-(}nNO2N`06-b#OZY6K;Jx0U1wlE zr;A(XI?ki%EOW9vN#kp0x^MeeY!=UA=O&H+Ud4;ay>k_1FmeJT&ysJ>(bs6UieNS;xys`7YlOX=NwQkx z*@NlM8`GUE^7uARn-juX7EvBMk)8U%z|C>{7v;G02jdRo=-TPW%lxj{f3TlnjrIP# zorC=hZOronyNBmb8t*1f0m+}R`|<%%eszN~Bv5__yh_qiKV0FT{kOYi zWw2qgb2SAHLK?mV3Qi~3q)EijpBt;#Ht6OWZXm}8sWSeP;sQ2@d^h}fwhsk{tA#U* z7q|IcU>3iqlo#T67Ef0B^eb-3GLG`B4Bb(aDARK1%6-MCK93tFU6uxsa*$#VV_;$<_PakPy4%}d>==!LCI}rHvJOMrguq# zay82%XBk`dYG8SG`Evm-i{{H&oH^g7Ac5CW9&HYHu?OAWBEL+moJ9t%Qu~%kW->6u zXN?USojJ(yRaP}M4_)WzZ%FSl=Y6zTbpE`X=8O2=`RJr~32+(4&T^f6~a#AKA2|@ zwb^GLD?E?SbGe6eH0IfG5&)*4NOS1X-XmA;wwAy`K0${nupW;86N3B;6OI15IBHC^ zElnQ*b<9Bm%w{*F?ZgoKfO6Sl*>>jmXS&GR7-riJY}+Kk2>HD+-KXAgkB$3OEMN~M zJdJPi-fViiDEefOJ%G^PL!U7k96o5^T-`6DEW^aiJP-496+2(%aXN;CBc#4FpDxC* zhhtMRhSJIIk~AM@tDBqY_whW+CwK4=8%-Cm+Hp4hCk}-(kG_xZmSdZ=gt{69%hZs# zR8~*2Ns`7PB`&ET8e7(lahcFqJIN|vuJZAG@iin)mc=-5yr_HXfK3xYqgzwfpt^`Uv$0ro}wTr#E;#UM^t? zF2m$9hLkrR-@wj~%aj3{8od6Ap>dX(-Ho$ymCzje5)Y8a-7w4j-~Tg7#P|xP1cS_~ z7pvLKS>7cJ*eA$f!F_sX{ob(efDC;Ze~IsH3)9b*l{m~4gcS5~mf5%Q_sMK^9iMDI zEgOw9*y3~r27Ej_IX_!Jhh4b^e+hf1r)w{R*5+&?4iC~eTg~$9pn7*Yy-K6>{ve-6 z%gzlyxVxpk`QzS1kX<-wa{U&Vg%&Q7MIVz3*hiDaL3(tZ%rE2kI=lf3W9K^gwlKLU zSYY}7_OJ0}@BPK;(fVCH&J&HPv5p1gVWDX=S+1&f zRmW-a@Yz)~H=G*Z!M;K|Ut`H_nyi-Nt64PpGM>TS7Zd~WxlLn3!t&b6W&O#gYC%>{ zN5hlD5y~d;zT(^%ONa4lfg181RspsYI|f-Q5(<>$Ydi~4I`NUg5czKmxr=AZqIseA z;p?I04Stzq_M!{}S+Yv&L@f33z-r9FJMfr;-U`MC7}N2wpdGC{ouS8x@H(eMt?5{f z`~N*=T1q)qQW5W9Ia`5|V;JI~nCB4VLW)mZN*G~`ADkqM&!83@uscoQZgrKppAH9qrc>C&OSpB; z&d2YLdi|r})4GEO#o0Jn%$mp!CUnfCQgtJs<=occcZcK4{bqB!i9 zWINZvDyj%gnZF0@tTIbd5EH3(a%;^KUxS1QzSF8TO?=>%VBXF-)-2J)r;@3ib5tau z9)(GW+UZsmNhl!5wNg9rs7OME;!42cclGesJt(6bfZx^Qeboa*+~3vu^Lh=Ec|`8s zr@z*;1qN<~8$(zh(>g}LY#?6o?BnP#pU&;$4kaBXM}!4T1Ooq8#rl+`Z~vPFR3w+? zXbFTgaD+=*F~&&1ICY%6voM&vR1O;7gvHK)?LUY*R(cmd|uFpj<6gLO$osOO|XqjVe8}fbcV!u(}N6XB?-8VaC)`#{u0B;I8 zGM4Yv=>$Hv`(vlOjw2`zZ1xP?hx8e1O`mz5ke=^L@SekW!}Iqo7`k=RZ^7U#7^8*f zqAmNMm0D>tC%PKHr1~1Y4<}vZfQ1JZB}ZHMw{W~CCqQ$ULIZGg^SVC@8bECeLlj0CA^S$ePN8nlbH|?SD>u! 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AC=6;AF=0.429;AN=14;BaseQRankSum=0.000;DP=7;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;MQ=29.00;MQ0=0;MQRankSum=-0.550;QD=16.70;ReadPosRankSum=0.937;EFF=DOWNSTREAM(MODIFIER||||85|FAM138A|protein_coding|CODING|ENST00000417324|),DOWNSTREAM(MODIFIER|||||FAM138A|processed_transcript|CODING|ENST00000461467|),DOWNSTREAM(MODIFIER|||||MIR1302-10|miRNA|NON_CODING|ENST00000408384|),EXON(MODIFIER|||||MIR1302-10|antisense|NON_CODING|ENST00000469289|1),INTRON(MODIFIER|||||MIR1302-10|antisense|NON_CODING|ENST00000473358|1),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000423562|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000430492|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000438504|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000488147|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000538476|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000243485|MIR1302-10|ENST00000473358|||||lincRNA|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000243485|MIR1302-10|ENST00000469289|1/2||||lincRNA|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000488147|||||unprocessed_pseudogene|1,downstream_gene_variant|||ENSG00000237613|FAM138A|ENST00000417324|||||lincRNA|1,downstream_gene_variant|||ENSG00000243485|MIR1302-10|ENST00000607096|||||miRNA|1,downstream_gene_variant|||ENSG00000237613|FAM138A|ENST00000461467|||||lincRNA|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000538476|||||unprocessed_pseudogene|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000438504|||||unprocessed_pseudogene|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000423562|||||unprocessed_pseudogene|1 +chr1 30860 . G C 54.3 . AC=3;AF=0.058;AN=52;BaseQRankSum=2.815;DP=61;Dels=0.00;FS=2.403;HRun=0;HaplotypeScore=0.6396;InbreedingCoeff=-0.0048;MQ=36.25;MQ0=0;MQRankSum=0.396;QD=13.57;ReadPosRankSum=2.148;EFF=DOWNSTREAM(MODIFIER||||85|FAM138A|protein_coding|CODING|ENST00000417324|),DOWNSTREAM(MODIFIER|||||FAM138A|processed_transcript|CODING|ENST00000461467|),DOWNSTREAM(MODIFIER|||||MIR1302-10|miRNA|NON_CODING|ENST00000408384|),INTRON(MODIFIER|||||MIR1302-10|antisense|NON_CODING|ENST00000469289|1),INTRON(MODIFIER|||||MIR1302-10|antisense|NON_CODING|ENST00000473358|2),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000423562|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000430492|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000438504|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000488147|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000538476|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000243485|MIR1302-10|ENST00000473358|||||lincRNA|1,intron_variant&nc_transcript_variant|||ENSG00000243485|MIR1302-10|ENST00000469289|||||lincRNA|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000488147|||||unprocessed_pseudogene|1,downstream_gene_variant|||ENSG00000237613|FAM138A|ENST00000417324|||||lincRNA|1,downstream_gene_variant|||ENSG00000243485|MIR1302-10|ENST00000607096|||||miRNA|1,downstream_gene_variant|||ENSG00000237613|FAM138A|ENST00000461467|||||lincRNA|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000538476|||||unprocessed_pseudogene|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000438504|||||unprocessed_pseudogene|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000423562|||||unprocessed_pseudogene|1 +chr1 30867 . CCT C 49.48 . AC=6;AF=0.107;AN=56;BaseQRankSum=0.900;DP=65;FS=0.000;HRun=0;HaplotypeScore=12.2055;InbreedingCoeff=-0.1167;MQ=36.09;MQ0=0;MQRankSum=1.148;QD=3.81;ReadPosRankSum=0.124;EFF=DOWNSTREAM(MODIFIER||||85|FAM138A|protein_coding|CODING|ENST00000417324|),DOWNSTREAM(MODIFIER|||||FAM138A|processed_transcript|CODING|ENST00000461467|),DOWNSTREAM(MODIFIER|||||MIR1302-10|miRNA|NON_CODING|ENST00000408384|),INTRON(MODIFIER|||||MIR1302-10|antisense|NON_CODING|ENST00000469289|1),INTRON(MODIFIER|||||MIR1302-10|antisense|NON_CODING|ENST00000473358|2),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000423562|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000430492|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000438504|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000488147|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000538476|);CSQ=intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000243485|MIR1302-10|ENST00000473358|||||lincRNA|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000243485|MIR1302-10|ENST00000469289|||||lincRNA|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000488147|||||unprocessed_pseudogene|1,downstream_gene_variant|||ENSG00000237613|FAM138A|ENST00000417324|||||lincRNA|1,downstream_gene_variant|||ENSG00000243485|MIR1302-10|ENST00000607096|||||miRNA|1,downstream_gene_variant|||ENSG00000237613|FAM138A|ENST00000461467|||||lincRNA|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000538476|||||unprocessed_pseudogene|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000438504|||||unprocessed_pseudogene|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000423562|||||unprocessed_pseudogene|1 +chr1 30895 . T C 51.79 . AC=4;AF=0.069;AN=58;BaseQRankSum=-0.230;DP=63;Dels=0.00;FS=11.041;HRun=0;HaplotypeScore=0.2091;InbreedingCoeff=-0.0237;MQ=36.09;MQ0=0;MQRankSum=1.212;QD=3.98;ReadPosRankSum=0.230;EFF=DOWNSTREAM(MODIFIER||||85|FAM138A|protein_coding|CODING|ENST00000417324|),DOWNSTREAM(MODIFIER|||||FAM138A|processed_transcript|CODING|ENST00000461467|),DOWNSTREAM(MODIFIER|||||MIR1302-10|miRNA|NON_CODING|ENST00000408384|),INTRON(MODIFIER|||||MIR1302-10|antisense|NON_CODING|ENST00000469289|1),INTRON(MODIFIER|||||MIR1302-10|antisense|NON_CODING|ENST00000473358|2),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000423562|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000430492|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000438504|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000488147|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000538476|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000243485|MIR1302-10|ENST00000473358|||||lincRNA|1,intron_variant&nc_transcript_variant|||ENSG00000243485|MIR1302-10|ENST00000469289|||||lincRNA|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000488147|||||unprocessed_pseudogene|1,downstream_gene_variant|||ENSG00000237613|FAM138A|ENST00000417324|||||lincRNA|1,downstream_gene_variant|||ENSG00000243485|MIR1302-10|ENST00000607096|||||miRNA|1,downstream_gene_variant|||ENSG00000237613|FAM138A|ENST00000461467|||||lincRNA|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000538476|||||unprocessed_pseudogene|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000438504|||||unprocessed_pseudogene|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000423562|||||unprocessed_pseudogene|1 +chr1 30923 . G T 601.49 . AC=30;AF=1.000;AN=30;DP=17;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=-0.1858;MQ=35.61;MQ0=0;QD=35.38;EFF=DOWNSTREAM(MODIFIER||||85|FAM138A|protein_coding|CODING|ENST00000417324|),DOWNSTREAM(MODIFIER|||||FAM138A|processed_transcript|CODING|ENST00000461467|),DOWNSTREAM(MODIFIER|||||MIR1302-10|miRNA|NON_CODING|ENST00000408384|),INTRON(MODIFIER|||||MIR1302-10|antisense|NON_CODING|ENST00000469289|1),INTRON(MODIFIER|||||MIR1302-10|antisense|NON_CODING|ENST00000473358|2),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000423562|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000430492|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000438504|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000488147|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000538476|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000243485|MIR1302-10|ENST00000473358|||||lincRNA|1,intron_variant&nc_transcript_variant|||ENSG00000243485|MIR1302-10|ENST00000469289|||||lincRNA|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000488147|||||unprocessed_pseudogene|1,downstream_gene_variant|||ENSG00000237613|FAM138A|ENST00000417324|||||lincRNA|1,downstream_gene_variant|||ENSG00000243485|MIR1302-10|ENST00000607096|||||miRNA|1,downstream_gene_variant|||ENSG00000237613|FAM138A|ENST00000461467|||||lincRNA|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000538476|||||unprocessed_pseudogene|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000438504|||||unprocessed_pseudogene|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000423562|||||unprocessed_pseudogene|1 +chr1 69270 . A G 2971.02 . AC=53;AF=0.946;AN=56;BaseQRankSum=1.675;DP=100;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0340;InbreedingCoeff=0.1553;MQ=31.06;MQ0=0;MQRankSum=-0.742;QD=31.61;ReadPosRankSum=-1.925;EFF=SYNONYMOUS_CODING(LOW|SILENT|tcA/tcG|S60|305|OR4F5|protein_coding|CODING|ENST00000335137|);CSQ=synonymous_variant|tcA/tcG|S|ENSG00000186092|OR4F5|ENST00000335137|1/1|||60/305|protein_coding|1 +chr1 69428 . T G 938.56 . AC=3;AF=0.0283;AN=106;BaseQRankSum=-0.638;DP=5013;DS;Dels=0.00;FS=2.607;HRun=0;HaplotypeScore=2.5603;InbreedingCoeff=0.3557;MQ=29.79;MQ0=0;MQRankSum=-5.524;QD=18.40;ReadPosRankSum=4.047;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tTt/tGt|F113C|305|OR4F5|protein_coding|CODING|ENST00000335137|);CSQ=missense_variant|tTt/tGt|F/C|ENSG00000186092|OR4F5|ENST00000335137|1/1|possibly_damaging(0.568)|deleterious(0)|113/305|protein_coding|1 +chr1 69511 . A G 101944.44 . AC=79;AF=0.878;AN=90;BaseQRankSum=-1.592;DP=3853;DS;Dels=0.00;FS=20.586;HRun=0;HaplotypeScore=3.0494;InbreedingCoeff=0.1357;MQ=33.24;MQ0=0;MQRankSum=-12.858;QD=26.51;ReadPosRankSum=-1.756;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Aca/Gca|T141A|305|OR4F5|protein_coding|CODING|ENST00000335137|);CSQ=missense_variant|Aca/Gca|T/A|ENSG00000186092|OR4F5|ENST00000335137|1/1|benign(0.003)|tolerated(0.63)|141/305|protein_coding|1 +chr1 69761 . A T 1026.71 . AC=8;AF=0.091;AN=88;BaseQRankSum=1.333;DP=574;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.3806;InbreedingCoeff=0.5887;MQ=32.18;MQ0=0;MQRankSum=-2.342;QD=21.39;ReadPosRankSum=-3.271;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAt/gTt|D224V|305|OR4F5|protein_coding|CODING|ENST00000335137|);CSQ=missense_variant|gAt/gTt|D/V|ENSG00000186092|OR4F5|ENST00000335137|1/1|benign(0.072)|tolerated(0.1)|224/305|protein_coding|1 +chr1 69871 . G A 82.97 . AC=2;AF=0.025;AN=80;BaseQRankSum=-0.646;DP=154;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.1095;InbreedingCoeff=0.1693;MQ=31.10;MQ0=0;MQRankSum=-0.154;QD=20.74;ReadPosRankSum=-0.292;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gat/Aat|D261N|305|OR4F5|protein_coding|CODING|ENST00000335137|);CSQ=missense_variant|Gat/Aat|D/N|ENSG00000186092|OR4F5|ENST00000335137|1/1|probably_damaging(0.968)|tolerated(0.06)|261/305|protein_coding|1 +chr1 69897 . T C 2544.15 . AC=52;AF=0.813;AN=64;BaseQRankSum=-0.912;DP=92;Dels=0.00;FS=58.362;HRun=1;HaplotypeScore=0.1549;InbreedingCoeff=0.0561;MQ=30.68;MQ0=0;MQRankSum=3.326;QD=28.59;ReadPosRankSum=1.059;EFF=SYNONYMOUS_CODING(LOW|SILENT|tcT/tcC|S269|305|OR4F5|protein_coding|CODING|ENST00000335137|);CSQ=synonymous_variant|tcT/tcC|S|ENSG00000186092|OR4F5|ENST00000335137|1/1|||269/305|protein_coding|1 +chr1 861008 . G C 1110.54 . AC=40;AF=0.870;AN=46;BaseQRankSum=1.889;DP=38;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=0.1855;MQ=56.89;MQ0=0;MQRankSum=1.660;QD=33.65;ReadPosRankSum=-1.946;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|1),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|1),UPSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1,upstream_gene_variant|||ENSG00000223764|RP11-54O7.3|ENST00000609207|||||retained_intron|1 +chr1 861605 . C T 52.33 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-0.110;DP=330;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.2194;InbreedingCoeff=-0.0688;MQ=57.00;MQ0=0;MQRankSum=-0.597;QD=10.47;ReadPosRankSum=-1.210;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|2),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|2),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|2);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 861630 . G A 5677.14 . AC=75;AF=0.7212;AN=104;BaseQRankSum=-3.827;DP=239;Dels=0.00;FS=4.448;HRun=0;HaplotypeScore=0.6050;InbreedingCoeff=0.2720;MQ=57.64;MQ0=0;MQRankSum=-2.334;QD=28.82;ReadPosRankSum=-1.755;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|2),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|2),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|2);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 861808 . A G 1231.98 . AC=34;AF=1.000;AN=34;DP=36;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0587;InbreedingCoeff=-0.1349;MQ=57.39;MQ0=0;QD=34.22;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|2),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|2),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|2);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 864938 . G A 38.62 . AC=4;AF=0.67;AN=6;BaseQRankSum=0.736;DP=3;Dels=0.00;FS=4.771;HRun=0;HaplotypeScore=0.0000;MQ=60.00;MQ0=0;MQRankSum=-0.736;QD=19.31;ReadPosRankSum=0.736;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|2),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|2),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|2);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 865219 . G A 271.52 . AC=14;AF=0.226;AN=62;BaseQRankSum=-0.335;DP=65;Dels=0.00;FS=1.303;HRun=0;HaplotypeScore=0.1417;InbreedingCoeff=0.1395;MQ=57.84;MQ0=0;MQRankSum=0.625;QD=14.29;ReadPosRankSum=-0.407;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|2),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|2),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|2);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 865367 . G T 48.93 . AC=2;AF=0.022;AN=90;BaseQRankSum=-0.637;DP=186;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3749;InbreedingCoeff=0.1476;MQ=55.90;MQ0=0;MQRankSum=2.051;QD=16.31;ReadPosRankSum=0.013;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|2),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|2),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|2);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 865435 . C G 566.25 . AC=2;AF=0.0185;AN=108;BaseQRankSum=1.312;DP=479;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6697;InbreedingCoeff=-0.0645;MQ=56.23;MQ0=0;MQRankSum=-0.467;QD=18.88;ReadPosRankSum=-0.530;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|2),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|2),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|2);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 865694 . C T 2783.07 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-1.642;DP=2811;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.6308;InbreedingCoeff=-0.0276;MQ=58.79;MQ0=0;MQRankSum=1.333;QD=13.71;ReadPosRankSum=0.518;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cac/Tac|H78Y|108|SAMD11|protein_coding|CODING|ENST00000437963|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cac/Tac|H78Y|178|SAMD11|protein_coding|CODING|ENST00000420190|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cac/Tac|H78Y|681|SAMD11|protein_coding|CODING|ENST00000342066|);CSQ=missense_variant|Cac/Tac|H/Y|ENSG00000187634|SAMD11|ENST00000420190|3/7|possibly_damaging(0.493)|tolerated(0.09)|78/179|protein_coding|1,missense_variant|tGc/tAc|C/Y|ENSG00000268179|AL645608.1|ENST00000598827|3/6|possibly_damaging(0.598)||18/112|protein_coding|1,missense_variant|Cac/Tac|H/Y|ENSG00000187634|SAMD11|ENST00000437963|3/5|possibly_damaging(0.493)|tolerated(0.12)|78/109|protein_coding|1,missense_variant|Cac/Tac|H/Y|ENSG00000187634|SAMD11|ENST00000342066|3/14|possibly_damaging(0.493)|tolerated(0.07)|78/681|protein_coding|1,missense_variant|Cac/Tac|H/Y|ENSG00000187634|SAMD11|ENST00000341065|1/12|probably_damaging(0.986)|deleterious(0)|2/589|protein_coding|1 +chr1 865738 . A G 389.63 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.098;DP=2190;Dels=0.00;FS=12.943;HRun=2;HaplotypeScore=1.1793;InbreedingCoeff=-0.0108;MQ=58.92;MQ0=0;MQRankSum=1.104;QD=9.28;ReadPosRankSum=0.259;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|3),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|3),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|3);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 866319 . G A 53469.99 . AC=118;AF=0.9833;AN=120;BaseQRankSum=1.591;DP=1731;Dels=0.00;FS=7.888;HRun=0;HaplotypeScore=1.0922;InbreedingCoeff=-0.0237;MQ=56.60;MQ0=0;MQRankSum=0.921;QD=30.89;ReadPosRankSum=-0.721;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|3),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|3),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|3);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 866511 . C CCCCT 58244.04 . AC=74;AF=0.6167;AN=120;BaseQRankSum=-16.318;DP=2662;FS=36.325;HRun=0;HaplotypeScore=234.1454;InbreedingCoeff=-0.0915;MQ=48.89;MQ0=0;MQRankSum=-26.717;QD=25.31;ReadPosRankSum=-3.550;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 866515 . T TCCCC 28452.17 . AC=52;AF=0.4333;AN=120;BaseQRankSum=20.767;DP=2538;FS=131.119;HRun=4;HaplotypeScore=272.7313;InbreedingCoeff=-0.6682;MQ=48.42;MQ0=0;MQRankSum=-29.484;QD=13.08;ReadPosRankSum=13.879;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 866517 . C G 804.92 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.085;DP=2503;Dels=0.00;FS=17.839;HRun=0;HaplotypeScore=16.1376;InbreedingCoeff=-0.0112;MQ=48.27;MQ0=0;MQRankSum=7.710;QD=18.29;ReadPosRankSum=2.729;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 866527 . A T 4017.89 . AC=45;AF=0.3750;AN=120;BaseQRankSum=11.973;DP=2186;Dels=0.00;FS=0.676;HRun=0;HaplotypeScore=3.9960;InbreedingCoeff=-0.5937;MQ=46.89;MQ0=0;MQRankSum=0.035;QD=2.40;ReadPosRankSum=-21.689;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 866557 . C T 127.71 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.174;DP=1561;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7669;InbreedingCoeff=-0.0117;MQ=49.50;MQ0=0;MQRankSum=3.050;QD=5.55;ReadPosRankSum=-0.277;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 866731 . G C 56.38 . AC=1;AF=0.011;AN=90;BaseQRankSum=-0.423;DP=139;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1598;InbreedingCoeff=-0.1061;MQ=53.72;MQ0=0;MQRankSum=0.762;QD=14.09;ReadPosRankSum=-0.131;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 866893 . T C 622.24 . AC=22;AF=0.500;AN=44;BaseQRankSum=-3.378;DP=42;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0452;InbreedingCoeff=0.1880;MQ=58.67;MQ0=0;MQRankSum=1.917;QD=23.93;ReadPosRankSum=-1.917;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 866920 . A G 1246.22 . AC=38;AF=1.000;AN=38;DP=41;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0415;InbreedingCoeff=-0.1594;MQ=58.06;MQ0=0;QD=32.80;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 866938 . G A 218.0 . AC=10;AF=0.278;AN=36;BaseQRankSum=1.425;DP=38;Dels=0.00;FS=4.047;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=0.1861;MQ=59.15;MQ0=0;MQRankSum=0.256;QD=18.17;ReadPosRankSum=-1.863;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 870645 . T C 290.54 . AC=16;AF=0.320;AN=50;BaseQRankSum=-0.425;DP=42;Dels=0.00;FS=3.173;HRun=0;HaplotypeScore=0.0800;InbreedingCoeff=0.0985;MQ=58.33;MQ0=0;MQRankSum=0.337;QD=13.21;ReadPosRankSum=1.918;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 870659 . G T 39.74 . AC=2;AF=0.040;AN=50;BaseQRankSum=1.431;DP=38;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0399;InbreedingCoeff=-0.0066;MQ=57.53;MQ0=0;MQRankSum=0.000;QD=13.25;ReadPosRankSum=1.202;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 870903 . T C 4527.15 . AC=105;AF=0.9722;AN=108;BaseQRankSum=-1.547;DP=151;Dels=0.01;FS=0.000;HRun=0;HaplotypeScore=0.3492;InbreedingCoeff=-0.0281;MQ=55.90;MQ0=0;MQRankSum=-1.281;QD=30.59;ReadPosRankSum=-1.215;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 871020 . G A 123.6 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.361;DP=922;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6728;InbreedingCoeff=-0.0124;MQ=58.46;MQ0=0;MQRankSum=0.878;QD=12.36;ReadPosRankSum=0.658;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 871042 . C CA 1121.53 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-3.932;DP=1196;FS=4.192;HRun=1;HaplotypeScore=68.0502;InbreedingCoeff=-0.0178;MQ=58.64;MQ0=0;MQRankSum=0.039;QD=20.77;ReadPosRankSum=1.478;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 871215 . C G 3347.1 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.247;DP=4311;Dels=0.00;FS=7.397;HRun=1;HaplotypeScore=1.9160;InbreedingCoeff=-0.0257;MQ=58.16;MQ0=0;MQRankSum=2.137;QD=13.44;ReadPosRankSum=0.832;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccG|P123|178|SAMD11|protein_coding|CODING|ENST00000420190|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccG|P123|681|SAMD11|protein_coding|CODING|ENST00000342066|),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,synonymous_variant|ccC/ccG|P|ENSG00000187634|SAMD11|ENST00000420190|5/7|||123/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,synonymous_variant|ccC/ccG|P|ENSG00000187634|SAMD11|ENST00000342066|5/14|||123/681|protein_coding|1,synonymous_variant|ccC/ccG|P|ENSG00000187634|SAMD11|ENST00000341065|3/12|||47/589|protein_coding|1 +chr1 871306 . G A 333.26 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.027;DP=2068;Dels=0.00;FS=3.159;HRun=0;HaplotypeScore=2.0683;InbreedingCoeff=-0.0097;MQ=57.05;MQ0=0;MQRankSum=0.683;QD=9.52;ReadPosRankSum=0.829;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|5),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|5),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 871334 . G T 28303.7 . AC=78;AF=0.6500;AN=120;BaseQRankSum=-4.675;DP=1477;Dels=0.00;FS=1.219;HRun=0;HaplotypeScore=0.8602;InbreedingCoeff=-0.1122;MQ=57.60;MQ0=0;MQRankSum=3.104;QD=21.15;ReadPosRankSum=-0.389;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|5),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|5),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 871406 . C T 373.38 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.644;DP=663;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.3801;InbreedingCoeff=-0.0188;MQ=58.43;MQ0=0;MQRankSum=0.382;QD=14.94;ReadPosRankSum=0.041;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|5),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|5),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 871513 . C T 90.88 . AC=2;AF=0.0196;AN=102;BaseQRankSum=0.045;DP=143;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1691;InbreedingCoeff=0.0569;MQ=57.57;MQ0=0;MQRankSum=-0.474;QD=22.72;ReadPosRankSum=-0.123;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|5),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|5),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1 +chr1 871683 . G A 69.81 . AC=6;AF=0.375;AN=16;BaseQRankSum=0.684;DP=8;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.2498;MQ=58.46;MQ0=0;MQRankSum=1.221;QD=23.27;ReadPosRankSum=0.684;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|5),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|5),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1 +chr1 874314 . G A 306.48 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.210;DP=904;Dels=0.00;FS=2.007;HRun=2;HaplotypeScore=0.6052;InbreedingCoeff=-0.0150;MQ=58.05;MQ0=0;MQRankSum=-1.582;QD=10.95;ReadPosRankSum=0.649;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|5),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|5),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 874415 . C T 459.1 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.071;DP=2210;Dels=0.00;FS=0.832;HRun=0;HaplotypeScore=1.8123;InbreedingCoeff=-0.0101;MQ=58.23;MQ0=0;MQRankSum=1.495;QD=12.08;ReadPosRankSum=0.643;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|5),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|5),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 874665 . G A 1190.84 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.904;DP=2083;Dels=0.00;FS=5.872;HRun=0;HaplotypeScore=1.2968;InbreedingCoeff=-0.0278;MQ=57.54;MQ0=0;MQRankSum=1.122;QD=14.35;ReadPosRankSum=0.856;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),SYNONYMOUS_CODING(LOW|SILENT|ctG/ctA|L177|178|SAMD11|protein_coding|CODING|ENST00000420190|),SYNONYMOUS_CODING(LOW|SILENT|ctG/ctA|L177|681|SAMD11|protein_coding|CODING|ENST00000342066|),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=synonymous_variant|ctG/ctA|L|ENSG00000187634|SAMD11|ENST00000420190|7/7|||177/179|protein_coding|1,synonymous_variant|ctG/ctA|L|ENSG00000187634|SAMD11|ENST00000342066|7/14|||177/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,synonymous_variant|ctG/ctA|L|ENSG00000187634|SAMD11|ENST00000455979|1/7|||4/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,synonymous_variant|ctG/ctA|L|ENSG00000187634|SAMD11|ENST00000341065|5/12|||101/589|protein_coding|1 +chr1 874816 . C CT 2431.73 . AC=13;AF=0.1083;AN=120;BaseQRankSum=-3.645;DP=2129;FS=0.000;HRun=1;HaplotypeScore=136.3097;InbreedingCoeff=0.2233;MQ=55.95;MQ0=0;MQRankSum=-0.937;QD=9.11;ReadPosRankSum=-4.095;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),FRAME_SHIFT(HIGH||-/T|-228?|681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,frameshift_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000342066|7/14|||228/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,frameshift_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000455979|1/7|||55/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 874817 . C T 196.11 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-3.463;DP=2129;Dels=0.00;FS=3.213;HRun=0;HaplotypeScore=3.1349;InbreedingCoeff=-0.0440;MQ=55.95;MQ0=0;MQRankSum=1.345;QD=2.84;ReadPosRankSum=-3.888;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCc/cTc|P228L|681|SAMD11|protein_coding|CODING|ENST00000342066|),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,missense_variant|cCc/cTc|P/L|ENSG00000187634|SAMD11|ENST00000342066|7/14|benign(0.003)|tolerated(0.26)|228/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,missense_variant|cCc/cTc|P/L|ENSG00000187634|SAMD11|ENST00000455979|1/7|benign(0.086)|tolerated(0.06)|55/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 874825 . C G 56.5 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.967;DP=2039;Dels=0.00;FS=3.631;HRun=0;HaplotypeScore=3.2093;InbreedingCoeff=-0.0100;MQ=55.77;MQ0=0;MQRankSum=0.586;QD=4.71;ReadPosRankSum=-3.947;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cac/Gac|H231D|681|SAMD11|protein_coding|CODING|ENST00000342066|),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,missense_variant|Cac/Gac|H/D|ENSG00000187634|SAMD11|ENST00000342066|7/14|benign(0)|tolerated(0.23)|231/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,missense_variant|Cac/Gac|H/D|ENSG00000187634|SAMD11|ENST00000455979|1/7|benign(0.001)|tolerated(0.22)|58/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 874826 . A G 43.49 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-4.114;DP=2017;Dels=0.00;FS=12.409;HRun=0;HaplotypeScore=2.9313;InbreedingCoeff=-0.0145;MQ=55.77;MQ0=0;MQRankSum=1.636;QD=3.62;ReadPosRankSum=-1.153;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cAc/cGc|H231R|681|SAMD11|protein_coding|CODING|ENST00000342066|),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,missense_variant|cAc/cGc|H/R|ENSG00000187634|SAMD11|ENST00000342066|7/14|benign(0)|tolerated(0.37)|231/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,missense_variant|cAc/cGc|H/R|ENSG00000187634|SAMD11|ENST00000455979|1/7|benign(0)|tolerated(0.36)|58/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 874864 . CT C 864.62 . AC=4;AF=0.0333;AN=120;BaseQRankSum=2.646;DP=1615;FS=13.206;HRun=1;HaplotypeScore=111.2623;InbreedingCoeff=-0.0402;MQ=54.48;MQ0=0;MQRankSum=-2.660;QD=5.84;ReadPosRankSum=-2.656;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 874878 . T C 30.73 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-11.417;DP=1417;Dels=0.00;FS=9.439;HRun=4;HaplotypeScore=1.6844;InbreedingCoeff=-0.0402;MQ=53.55;MQ0=0;MQRankSum=0.037;QD=0.29;ReadPosRankSum=-3.513;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 874927 . A G 459.72 . AC=5;AF=0.0439;AN=114;BaseQRankSum=2.529;DP=728;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5050;InbreedingCoeff=0.1366;MQ=49.13;MQ0=0;MQRankSum=-1.720;QD=7.93;ReadPosRankSum=0.425;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 874933 . G A 71.83 . AC=2;AF=0.0175;AN=114;BaseQRankSum=-2.598;DP=677;Dels=0.00;FS=12.021;HRun=0;HaplotypeScore=0.5171;InbreedingCoeff=0.3582;MQ=48.55;MQ0=0;MQRankSum=-2.054;QD=17.96;ReadPosRankSum=-0.568;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 874949 . G GAC 180.07 . AC=12;AF=0.1091;AN=110;BaseQRankSum=4.227;DP=513;FS=1.980;HRun=0;HaplotypeScore=80.8744;InbreedingCoeff=-0.1326;MQ=45.53;MQ0=0;MQRankSum=0.812;QD=1.55;ReadPosRankSum=-6.278;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 874950 . T C 171.91 . AC=11;AF=0.1000;AN=110;BaseQRankSum=-0.909;DP=513;Dels=0.00;FS=2.116;HRun=3;HaplotypeScore=3.7881;InbreedingCoeff=-0.1427;MQ=45.53;MQ0=0;MQRankSum=5.553;QD=1.55;ReadPosRankSum=-4.349;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 874950 . T TCCCTGGAGGACC 52769.69 . AC=82;AF=0.7455;AN=110;BaseQRankSum=-5.074;DP=556;FS=0.992;HRun=0;HaplotypeScore=89.5226;InbreedingCoeff=-0.2281;MQ=43.74;MQ0=0;MQRankSum=-13.823;QD=95.42;ReadPosRankSum=1.894;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 874955 . C G 162.26 . AC=15;AF=0.1364;AN=110;BaseQRankSum=-1.052;DP=411;Dels=0.00;FS=15.434;HRun=0;HaplotypeScore=3.6422;InbreedingCoeff=-0.1058;MQ=46.15;MQ0=0;MQRankSum=4.641;QD=1.62;ReadPosRankSum=-7.007;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 875159 . AGCCAGTGGACGCCGACCT A 727.04 . AC=15;AF=0.500;AN=30;BaseQRankSum=3.932;DP=27;FS=0.000;HRun=0;HaplotypeScore=24.4581;InbreedingCoeff=0.3261;MQ=51.25;MQ0=0;MQRankSum=-2.825;QD=80.78;ReadPosRankSum=2.534;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 876498 . GA G 1427.8 . AC=48;AF=0.4000;AN=120;BaseQRankSum=-5.371;DP=794;FS=21.973;HRun=1;HaplotypeScore=83.2253;InbreedingCoeff=-0.3157;MQ=58.06;MQ0=0;MQRankSum=0.970;QD=2.48;ReadPosRankSum=-12.769;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|1),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),INTRON(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|1),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000474461|1/4||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 876499 . A G 22430.37 . AC=114;AF=0.9500;AN=120;BaseQRankSum=2.512;DP=794;Dels=0.01;FS=0.566;HRun=6;HaplotypeScore=0.6449;InbreedingCoeff=-0.0691;MQ=58.06;MQ0=0;MQRankSum=0.580;QD=28.25;ReadPosRankSum=0.704;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|1),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),INTRON(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|1),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|1/4||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 876595 . C T 278.95 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.357;DP=1272;Dels=0.00;FS=1.349;HRun=0;HaplotypeScore=0.7550;InbreedingCoeff=-0.0197;MQ=57.15;MQ0=0;MQRankSum=0.060;QD=13.28;ReadPosRankSum=-2.131;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),EXON(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|2),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ccc/Tcc|P260S|681|SAMD11|protein_coding|CODING|ENST00000342066|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,missense_variant|Ccc/Tcc|P/S|ENSG00000187634|SAMD11|ENST00000342066|8/14|probably_damaging(0.987)|deleterious(0)|260/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|1/4||||retained_intron|1,missense_variant|Ccc/Tcc|P/S|ENSG00000187634|SAMD11|ENST00000455979|2/7|probably_damaging(0.999)|deleterious(0)|87/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000478729|2/3||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,missense_variant|Ccc/Tcc|P/S|ENSG00000187634|SAMD11|ENST00000341065|6/12|probably_damaging(0.999)|tolerated(0.05)|168/589|protein_coding|1 +chr1 876908 . G A 134.68 . AC=1;AF=0.013;AN=80;BaseQRankSum=1.386;DP=184;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.2023;InbreedingCoeff=-0.0861;MQ=56.39;MQ0=0;MQRankSum=0.341;QD=14.96;ReadPosRankSum=1.908;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|8),INTRON(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|2),INTRON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|1),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 877147 . G A 43.79 . AC=6;AF=0.273;AN=22;BaseQRankSum=-0.925;DP=17;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0000;InbreedingCoeff=0.2867;MQ=52.81;MQ0=0;MQRankSum=0.185;QD=14.60;ReadPosRankSum=0.740;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|8),INTRON(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|2),INTRON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|1),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 877715 . C G 5039.18 . AC=98;AF=0.9423;AN=104;BaseQRankSum=1.292;DP=191;Dels=0.00;FS=2.185;HRun=3;HaplotypeScore=0.2807;InbreedingCoeff=-0.0534;MQ=53.91;MQ0=0;MQRankSum=-0.346;QD=26.66;ReadPosRankSum=0.008;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|1),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|9),INTRON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|1),INTRON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|2);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000464948|1/2||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 877782 . C G 59.43 . AC=4;AF=0.0357;AN=112;BaseQRankSum=-0.568;DP=212;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0665;InbreedingCoeff=0.0350;MQ=54.31;MQ0=0;MQRankSum=1.437;QD=5.40;ReadPosRankSum=0.130;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|1),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|9),INTRON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|1),INTRON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|2);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000464948|1/2||||retained_intron|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 877831 . T C 5022.11 . AC=102;AF=1.0000;AN=102;BaseQRankSum=1.709;DP=173;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.1329;InbreedingCoeff=-0.0991;MQ=53.24;MQ0=0;MQRankSum=1.638;QD=29.20;ReadPosRankSum=-0.772;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|1),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|2),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|3),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tgg/Cgg|W343R|681|SAMD11|protein_coding|CODING|ENST00000342066|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,missense_variant|Tgg/Cgg|W/R|ENSG00000187634|SAMD11|ENST00000342066|10/14|benign(0)|tolerated(1)|343/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000464948|1/2||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000466827|2/2||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|3/4||||retained_intron|1,missense_variant|Tgg/Cgg|W/R|ENSG00000187634|SAMD11|ENST00000455979|4/7|benign(0)|tolerated(1)|170/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,missense_variant|Tgg/Cgg|W/R|ENSG00000187634|SAMD11|ENST00000341065|8/12|benign(0)|tolerated(1)|251/589|protein_coding|1 +chr1 878314 . G C 762.28 . AC=11;AF=0.0917;AN=120;BaseQRankSum=0.711;DP=450;Dels=0.00;FS=2.520;HRun=0;HaplotypeScore=0.3685;InbreedingCoeff=-0.1321;MQ=57.76;MQ0=0;MQRankSum=1.717;QD=9.30;ReadPosRankSum=1.114;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|4),SYNONYMOUS_CODING(LOW|SILENT|ggG/ggC|G480|681|SAMD11|protein_coding|CODING|ENST00000342066|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,synonymous_variant|ggG/ggC|G|ENSG00000187634|SAMD11|ENST00000342066|11/14|||480/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|4/4||||retained_intron|1,synonymous_variant|ggG/ggC|G|ENSG00000187634|SAMD11|ENST00000455979|5/7|||307/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,synonymous_variant|ggG/ggC|G|ENSG00000187634|SAMD11|ENST00000341065|9/12|||388/589|protein_coding|1 +chr1 878331 . C T 170.27 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.516;DP=507;Dels=0.00;FS=13.852;HRun=0;HaplotypeScore=0.4065;InbreedingCoeff=-0.0252;MQ=57.83;MQ0=0;MQRankSum=0.921;QD=21.28;ReadPosRankSum=-1.361;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|4),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P486L|681|SAMD11|protein_coding|CODING|ENST00000342066|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,missense_variant|cCg/cTg|P/L|ENSG00000187634|SAMD11|ENST00000342066|11/14|benign(0.007)|tolerated(0.12)|486/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|4/4||||retained_intron|1,missense_variant|cCg/cTg|P/L|ENSG00000187634|SAMD11|ENST00000455979|5/7|benign(0.029)|tolerated(0.09)|313/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,missense_variant|cCg/cTg|P/L|ENSG00000187634|SAMD11|ENST00000341065|9/12|benign(0.006)|tolerated(0.18)|394/589|protein_coding|1 +chr1 878516 . GA G 725.28 . AC=4;AF=0.0333;AN=120;BaseQRankSum=4.091;DP=1587;FS=6.039;HRun=5;HaplotypeScore=109.1933;InbreedingCoeff=-0.0395;MQ=57.40;MQ0=0;MQRankSum=0.773;QD=10.67;ReadPosRankSum=1.454;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|11);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 878761 . A AG 102.98 . AC=2;AF=0.0167;AN=120;BaseQRankSum=0.641;DP=955;FS=3.593;HRun=7;HaplotypeScore=63.6389;InbreedingCoeff=-0.0174;MQ=56.57;MQ0=0;MQRankSum=0.356;QD=4.90;ReadPosRankSum=-0.194;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|12);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,splice_region_variant&intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,splice_region_variant&intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,splice_region_variant&intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 878764 . G C 139.53 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.470;DP=938;Dels=0.00;FS=7.307;HRun=0;HaplotypeScore=0.9091;InbreedingCoeff=-0.0119;MQ=56.61;MQ0=0;MQRankSum=1.683;QD=9.97;ReadPosRankSum=2.283;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|12);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 878904 . A C 629.76 . AC=27;AF=0.270;AN=100;BaseQRankSum=-10.394;DP=328;Dels=0.01;FS=66.455;HRun=4;HaplotypeScore=5.0793;InbreedingCoeff=-0.3279;MQ=45.67;MQ0=0;MQRankSum=-7.168;QD=2.59;ReadPosRankSum=7.847;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|12);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 878906 . CTTT C 498.81 . AC=35;AF=0.407;AN=86;BaseQRankSum=-0.896;DP=307;FS=10.838;HRun=22;HaplotypeScore=48.3314;InbreedingCoeff=0.1154;MQ=44.42;MQ0=0;MQRankSum=-1.401;QD=2.60;ReadPosRankSum=2.748;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|12);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 879195 . C T 744.73 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.733;DP=2228;Dels=0.00;FS=2.345;HRun=0;HaplotypeScore=2.3431;InbreedingCoeff=-0.0106;MQ=57.41;MQ0=0;MQRankSum=1.176;QD=11.82;ReadPosRankSum=0.278;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|13);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,missense_variant|Cgc/Tgc|R/C|ENSG00000187634|SAMD11|ENST00000455979|7/7|benign(0.047)|tolerated(0.15)|430/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 879317 . C T 1801.69 . AC=5;AF=0.0417;AN=120;BaseQRankSum=0.490;DP=1511;Dels=0.00;FS=0.480;HRun=0;HaplotypeScore=0.8559;InbreedingCoeff=-0.0439;MQ=57.96;MQ0=0;MQRankSum=-0.001;QD=13.15;ReadPosRankSum=0.731;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),SYNONYMOUS_CODING(LOW|SILENT|taC/taT|Y610|681|SAMD11|protein_coding|CODING|ENST00000342066|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,synonymous_variant|taC/taT|Y|ENSG00000187634|SAMD11|ENST00000342066|14/14|||610/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,synonymous_variant|taC/taT|Y|ENSG00000187634|SAMD11|ENST00000455979|7/7|||470/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,synonymous_variant|taC/taT|Y|ENSG00000187634|SAMD11|ENST00000341065|12/12|||518/589|protein_coding|1 +chr1 879376 . G A 35.51 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.159;DP=1323;Dels=0.00;FS=4.217;HRun=1;HaplotypeScore=0.6368;InbreedingCoeff=-0.0128;MQ=58.52;MQ0=0;MQRankSum=2.465;QD=2.96;ReadPosRankSum=0.209;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGa/cAa|R630Q|681|SAMD11|protein_coding|CODING|ENST00000342066|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,missense_variant|cGa/cAa|R/Q|ENSG00000187634|SAMD11|ENST00000342066|14/14|benign(0.048)|tolerated(0.13)|630/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,missense_variant|cGa/cAa|R/Q|ENSG00000187634|SAMD11|ENST00000455979|7/7|benign(0.024)|tolerated(0.19)|490/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,missense_variant|cGa/cAa|R/Q|ENSG00000187634|SAMD11|ENST00000341065|12/12|benign(0.012)|tolerated(0.16)|538/589|protein_coding|1 +chr1 879482 . G C 516.68 . AC=1;AF=0.0088;AN=114;BaseQRankSum=2.415;DP=1065;Dels=0.00;FS=3.604;HRun=0;HaplotypeScore=0.8043;InbreedingCoeff=-0.0358;MQ=59.14;MQ0=0;MQRankSum=0.854;QD=17.82;ReadPosRankSum=-1.823;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),SYNONYMOUS_CODING(LOW|SILENT|ggG/ggC|G665|681|SAMD11|protein_coding|CODING|ENST00000342066|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,synonymous_variant|ggG/ggC|G|ENSG00000187634|SAMD11|ENST00000342066|14/14|||665/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,synonymous_variant|ggG/ggC|G|ENSG00000187634|SAMD11|ENST00000455979|7/7|||525/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,synonymous_variant|ggG/ggC|G|ENSG00000187634|SAMD11|ENST00000341065|12/12|||573/589|protein_coding|1 +chr1 879676 . G A 6945.69 . AC=71;AF=0.934;AN=76;BaseQRankSum=-1.592;DP=208;Dels=0.00;FS=0.000;HRun=3;HaplotypeScore=0.0908;InbreedingCoeff=0.0037;MQ=56.70;MQ0=0;MQRankSum=-0.599;QD=33.55;ReadPosRankSum=-1.965;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|17),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|5),UTR_3_PRIME(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|14),UTR_3_PRIME(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|19);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000187634|SAMD11|ENST00000342066|14/14|||-/681|protein_coding|1,3_prime_UTR_variant|||ENSG00000188976|NOC2L|ENST00000327044|19/19|||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|5/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|17/17||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187634|SAMD11|ENST00000341065|12/12|||-/589|protein_coding|1 +chr1 879687 . T C 5518.2 . AC=65;AF=0.956;AN=68;BaseQRankSum=0.725;DP=166;Dels=0.00;FS=1.578;HRun=1;HaplotypeScore=0.1048;InbreedingCoeff=0.0533;MQ=56.43;MQ0=0;MQRankSum=0.068;QD=33.44;ReadPosRankSum=-0.128;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|17),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|5),UTR_3_PRIME(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|14),UTR_3_PRIME(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|19);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000187634|SAMD11|ENST00000342066|14/14|||-/681|protein_coding|1,3_prime_UTR_variant|||ENSG00000188976|NOC2L|ENST00000327044|19/19|||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|5/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|17/17||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187634|SAMD11|ENST00000341065|12/12|||-/589|protein_coding|1 +chr1 879911 . G A 413.77 . AC=3;AF=0.0254;AN=118;BaseQRankSum=-0.760;DP=308;Dels=0.00;FS=1.528;HRun=1;HaplotypeScore=0.1788;InbreedingCoeff=-0.0710;MQ=57.83;MQ0=0;MQRankSum=0.760;QD=15.32;ReadPosRankSum=0.384;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|17),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|5),UTR_3_PRIME(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|14),UTR_3_PRIME(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|19);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000187634|SAMD11|ENST00000342066|14/14|||-/681|protein_coding|1,3_prime_UTR_variant|||ENSG00000188976|NOC2L|ENST00000327044|19/19|||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|5/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|17/17||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187634|SAMD11|ENST00000341065|12/12|||-/589|protein_coding|1 +chr1 880110 . G A 149.9 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.394;DP=1847;Dels=0.00;FS=1.624;HRun=0;HaplotypeScore=0.9203;InbreedingCoeff=-0.0094;MQ=57.47;MQ0=0;MQRankSum=1.220;QD=6.25;ReadPosRankSum=0.988;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|17),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|5),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D738|749|NOC2L|protein_coding|CODING|ENST00000327044|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,synonymous_variant|gaC/gaT|D|ENSG00000188976|NOC2L|ENST00000327044|19/19|||738/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|5/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|17/17||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 880238 . A G 43084.12 . AC=114;AF=0.9500;AN=120;BaseQRankSum=-0.613;DP=1203;Dels=0.00;FS=12.344;HRun=1;HaplotypeScore=1.0677;InbreedingCoeff=-0.0525;MQ=58.52;MQ0=0;MQRankSum=-1.859;QD=35.81;ReadPosRankSum=-0.535;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|18),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|4);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 880258 . TAA T 1233.06 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.890;DP=1382;FS=2.604;HRun=2;HaplotypeScore=131.0286;InbreedingCoeff=-0.0085;MQ=58.89;MQ0=0;MQRankSum=1.259;QD=20.55;ReadPosRankSum=-3.041;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|18),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|4);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 880367 . G A 795.59 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.447;DP=3095;Dels=0.00;FS=7.414;HRun=0;HaplotypeScore=2.5302;InbreedingCoeff=-0.0084;MQ=58.79;MQ0=0;MQRankSum=0.556;QD=10.61;ReadPosRankSum=0.729;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|18),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|4);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 880390 . C A 4207.41 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-1.360;DP=3838;Dels=0.00;FS=18.983;HRun=0;HaplotypeScore=2.5364;InbreedingCoeff=-0.0345;MQ=58.94;MQ0=0;MQRankSum=0.010;QD=14.76;ReadPosRankSum=1.464;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|18),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|4);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 880483 . A G 739.51 . AC=2;AF=0.0167;AN=120;BaseQRankSum=1.408;DP=5022;DS;Dels=0.00;FS=13.337;HRun=0;HaplotypeScore=2.6561;InbreedingCoeff=-0.0170;MQ=59.08;MQ0=0;MQRankSum=0.004;QD=12.75;ReadPosRankSum=-2.856;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|16),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|4),SYNONYMOUS_CODING(LOW|SILENT|gaT/gaC|D699|749|NOC2L|protein_coding|CODING|ENST00000327044|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,synonymous_variant|gaT/gaC|D|ENSG00000188976|NOC2L|ENST00000327044|18/19|||699/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|4/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|16/17||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 880639 . TC T 8144.14 . AC=31;AF=0.2583;AN=120;BaseQRankSum=8.550;DP=921;FS=0.348;HRun=2;HaplotypeScore=54.1306;InbreedingCoeff=-0.1017;MQ=58.66;MQ0=0;MQRankSum=1.840;QD=17.70;ReadPosRankSum=1.996;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|17),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|15),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|3);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 880751 . A G 807.9 . AC=4;AF=0.0333;AN=120;BaseQRankSum=1.558;DP=1112;Dels=0.00;FS=1.129;HRun=1;HaplotypeScore=0.7010;InbreedingCoeff=0.4309;MQ=58.97;MQ0=0;MQRankSum=3.000;QD=18.79;ReadPosRankSum=0.469;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|2),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|17),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|15),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|3);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000496938|2/2||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 881027 . C G 2780.96 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.239;DP=6197;DS;Dels=0.00;FS=8.993;HRun=0;HaplotypeScore=3.1641;InbreedingCoeff=-0.0084;MQ=59.26;MQ0=0;MQRankSum=2.376;QD=14.56;ReadPosRankSum=-0.209;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|15),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|3),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gac/Cac|D642H|749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,missense_variant|Gac/Cac|D/H|ENSG00000188976|NOC2L|ENST00000327044|17/19|probably_damaging(0.928)|deleterious(0)|642/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|3/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|15/17||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 881068 . G A 311.66 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.528;DP=3714;DS;Dels=0.00;FS=2.094;HRun=1;HaplotypeScore=2.7083;InbreedingCoeff=-0.0084;MQ=58.85;MQ0=0;MQRankSum=2.312;QD=14.17;ReadPosRankSum=-1.657;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|14),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|2),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 881214 . A G 121.77 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-0.359;DP=622;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.2865;InbreedingCoeff=-0.0200;MQ=58.09;MQ0=0;MQRankSum=-1.890;QD=15.22;ReadPosRankSum=-1.501;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|14),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|2),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 881278 . T C 73.18 . AC=5;AF=0.0472;AN=106;BaseQRankSum=-5.288;DP=225;Dels=0.00;FS=14.303;HRun=2;HaplotypeScore=0.2150;InbreedingCoeff=-0.1298;MQ=55.77;MQ0=0;MQRankSum=-0.007;QD=2.36;ReadPosRankSum=-0.143;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|14),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|2),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 881377 . G A 198.25 . AC=3;AF=0.0259;AN=116;BaseQRankSum=0.943;DP=299;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1931;InbreedingCoeff=-0.0772;MQ=57.25;MQ0=0;MQRankSum=1.239;QD=11.01;ReadPosRankSum=1.934;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|14),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|2),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 881512 . G T 329.45 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.087;DP=1882;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.4710;InbreedingCoeff=-0.0118;MQ=58.18;MQ0=0;MQRankSum=0.210;QD=11.36;ReadPosRankSum=-0.792;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|14),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|2),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 881627 . G A 65058.1 . AC=69;AF=0.5750;AN=120;BaseQRankSum=-0.436;DP=4175;Dels=0.00;FS=7.028;HRun=1;HaplotypeScore=3.6325;InbreedingCoeff=0.0788;MQ=58.73;MQ0=0;MQRankSum=0.133;QD=20.93;ReadPosRankSum=1.488;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|14),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|2),SYNONYMOUS_CODING(LOW|SILENT|Ctg/Ttg|L615|749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,synonymous_variant|Ctg/Ttg|L|ENSG00000188976|NOC2L|ENST00000327044|16/19|||615/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|2/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|14/17||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 881734 . T C 2395.2 . AC=3;AF=0.0250;AN=120;BaseQRankSum=1.087;DP=3324;Dels=0.00;FS=13.773;HRun=0;HaplotypeScore=2.3394;InbreedingCoeff=-0.0275;MQ=58.22;MQ0=0;MQRankSum=4.896;QD=14.34;ReadPosRankSum=1.344;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|15),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|13),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|1),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 881894 . G A 502.77 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.087;DP=3111;Dels=0.00;FS=0.806;HRun=0;HaplotypeScore=1.9434;InbreedingCoeff=-0.0084;MQ=55.95;MQ0=0;MQRankSum=4.130;QD=7.62;ReadPosRankSum=-0.465;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|13),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCc/gTc|A564V|749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,missense_variant|gCc/gTc|A/V|ENSG00000188976|NOC2L|ENST00000327044|15/19|probably_damaging(0.911)|deleterious(0)|564/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|1/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|13/17||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 881918 . G A 4148.23 . AC=6;AF=0.0500;AN=120;BaseQRankSum=0.336;DP=2717;Dels=0.00;FS=3.311;HRun=1;HaplotypeScore=1.8603;InbreedingCoeff=-0.0526;MQ=57.50;MQ0=0;MQRankSum=10.868;QD=13.38;ReadPosRankSum=2.821;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|13),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCg/tTg|S556L|749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,missense_variant|tCg/tTg|S/L|ENSG00000188976|NOC2L|ENST00000327044|15/19|benign(0.311)|deleterious(0.03)|556/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|1/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|13/17||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 882033 . G A 7803.05 . AC=29;AF=0.2417;AN=120;BaseQRankSum=-1.083;DP=803;Dels=0.00;FS=0.805;HRun=1;HaplotypeScore=0.5525;InbreedingCoeff=-0.0708;MQ=56.82;MQ0=0;MQRankSum=1.538;QD=18.99;ReadPosRankSum=-1.493;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|1),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|14),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|12),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|1/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 883625 . A G 54754.53 . AC=120;AF=1.0000;AN=120;DP=1639;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=1.0098;InbreedingCoeff=-0.0052;MQ=58.06;MQ0=0;QD=33.41;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|13),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|11),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 883699 . C T 453.1 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.101;DP=923;Dels=0.00;FS=14.883;HRun=0;HaplotypeScore=0.5131;InbreedingCoeff=-0.0294;MQ=56.96;MQ0=0;MQRankSum=3.137;QD=12.25;ReadPosRankSum=2.276;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|13),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|11),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 883750 . T C 877.5 . AC=3;AF=0.0250;AN=120;BaseQRankSum=1.693;DP=1077;Dels=0.00;FS=5.318;HRun=3;HaplotypeScore=0.3559;InbreedingCoeff=-0.0265;MQ=57.03;MQ0=0;MQRankSum=4.377;QD=17.21;ReadPosRankSum=1.955;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|13),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|11),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 883960 . G A 483.87 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.195;DP=3321;Dels=0.00;FS=1.899;HRun=0;HaplotypeScore=1.8090;InbreedingCoeff=-0.0084;MQ=57.94;MQ0=0;MQRankSum=2.447;QD=10.30;ReadPosRankSum=1.425;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|11),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N489|749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,synonymous_variant|aaC/aaT|N|ENSG00000188976|NOC2L|ENST00000327044|13/19|||489/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|11/17||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 884013 . G A 359.69 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.049;DP=1892;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.9736;InbreedingCoeff=-0.0084;MQ=57.09;MQ0=0;MQRankSum=2.918;QD=14.99;ReadPosRankSum=0.004;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 884035 . G GCC 125.11 . AC=12;AF=0.1000;AN=120;BaseQRankSum=10.429;DP=1507;FS=5.790;HRun=1;HaplotypeScore=118.1286;InbreedingCoeff=-0.1150;MQ=55.10;MQ0=0;MQRankSum=-9.464;QD=0.53;ReadPosRankSum=-1.582;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant&feature_elongation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 884040 . AC A 54.58 . AC=10;AF=0.0833;AN=120;BaseQRankSum=10.370;DP=1485;FS=5.837;HRun=3;HaplotypeScore=105.2143;InbreedingCoeff=-0.0972;MQ=54.77;MQ0=0;MQRankSum=-6.138;QD=0.29;ReadPosRankSum=-3.294;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 884091 . C G 9412.98 . AC=59;AF=0.4917;AN=120;BaseQRankSum=0.144;DP=830;Dels=0.00;FS=3.690;HRun=1;HaplotypeScore=1.5824;InbreedingCoeff=-0.8297;MQ=49.85;MQ0=0;MQRankSum=3.025;QD=11.58;ReadPosRankSum=6.853;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 884101 . A C 9419.59 . AC=54;AF=0.4500;AN=120;BaseQRankSum=12.324;DP=697;Dels=0.00;FS=0.000;HRun=3;HaplotypeScore=1.3334;InbreedingCoeff=-0.7661;MQ=48.44;MQ0=0;MQRankSum=-6.895;QD=14.45;ReadPosRankSum=-14.010;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 884111 . T C 106.0 . AC=12;AF=0.1017;AN=118;BaseQRankSum=-1.701;DP=405;Dels=0.00;FS=32.333;HRun=2;HaplotypeScore=0.1962;InbreedingCoeff=-0.1423;MQ=53.01;MQ0=0;MQRankSum=1.879;QD=1.39;ReadPosRankSum=-6.750;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 884426 . A AACAGCAAAG 1975.5 . AC=28;AF=1.000;AN=28;DP=24;FS=0.000;HRun=0;HaplotypeScore=8.5806;InbreedingCoeff=-0.1820;MQ=37.89;MQ0=0;QD=98.77;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant&feature_elongation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 +chr1 886006 . T C 1030.61 . AC=32;AF=1.000;AN=32;DP=31;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=-0.1369;MQ=49.82;MQ0=0;QD=33.25;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 886049 . ACAG A 1495.02 . AC=34;AF=0.944;AN=36;BaseQRankSum=1.722;DP=33;FS=2.691;HRun=0;HaplotypeScore=10.4318;InbreedingCoeff=0.1010;MQ=41.07;MQ0=0;MQRankSum=-2.098;QD=49.83;ReadPosRankSum=0.344;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 886179 . CA C 1612.0 . AC=47;AF=0.870;AN=54;BaseQRankSum=3.364;DP=49;FS=8.293;HRun=1;HaplotypeScore=18.7687;InbreedingCoeff=0.1732;MQ=36.71;MQ0=0;MQRankSum=-3.123;QD=36.64;ReadPosRankSum=0.259;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 886182 . TG T 1532.98 . AC=47;AF=0.870;AN=54;BaseQRankSum=3.246;DP=46;FS=5.278;HRun=1;HaplotypeScore=18.4565;InbreedingCoeff=0.1421;MQ=34.65;MQ0=0;MQRankSum=-2.960;QD=36.50;ReadPosRankSum=0.184;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 886186 . C CAG 2268.91 . AC=57;AF=0.891;AN=64;BaseQRankSum=3.232;DP=55;FS=6.052;HRun=0;HaplotypeScore=17.8027;InbreedingCoeff=0.1016;MQ=34.53;MQ0=0;MQRankSum=-3.122;QD=44.49;ReadPosRankSum=0.055;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 886384 . G A 434.04 . AC=4;AF=0.0333;AN=120;BaseQRankSum=0.231;DP=475;Dels=0.00;FS=8.266;HRun=0;HaplotypeScore=0.2516;InbreedingCoeff=-0.0534;MQ=58.22;MQ0=0;MQRankSum=1.803;QD=11.42;ReadPosRankSum=-0.069;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 886409 . G C 225.93 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-0.047;DP=629;Dels=0.00;FS=1.803;HRun=2;HaplotypeScore=0.3393;InbreedingCoeff=-0.0500;MQ=58.47;MQ0=0;MQRankSum=0.500;QD=5.79;ReadPosRankSum=-0.845;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 886477 . G A 1109.56 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.561;DP=1412;Dels=0.00;FS=5.360;HRun=0;HaplotypeScore=1.3637;InbreedingCoeff=-0.0325;MQ=58.75;MQ0=0;MQRankSum=0.602;QD=11.56;ReadPosRankSum=-1.213;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 886492 . C T 237.45 . AC=2;AF=0.0167;AN=120;BaseQRankSum=0.074;DP=1595;Dels=0.00;FS=5.466;HRun=0;HaplotypeScore=0.8808;InbreedingCoeff=0.7925;MQ=58.78;MQ0=0;MQRankSum=0.397;QD=21.59;ReadPosRankSum=-0.482;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 886653 . G A 558.63 . AC=2;AF=0.0169;AN=118;BaseQRankSum=1.368;DP=1520;Dels=0.00;FS=21.500;HRun=0;HaplotypeScore=1.1951;InbreedingCoeff=-0.0285;MQ=58.36;MQ0=0;MQRankSum=0.809;QD=15.52;ReadPosRankSum=-1.905;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|11),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|9),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 886788 . G A 9988.89 . AC=98;AF=0.8305;AN=118;BaseQRankSum=-1.367;DP=334;Dels=0.00;FS=18.012;HRun=1;HaplotypeScore=0.1988;InbreedingCoeff=0.2452;MQ=57.31;MQ0=0;MQRankSum=4.299;QD=32.86;ReadPosRankSum=-0.071;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|11),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|9),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 886817 . C T 6332.01 . AC=90;AF=0.8182;AN=110;BaseQRankSum=-1.237;DP=216;Dels=0.00;FS=12.660;HRun=3;HaplotypeScore=0.3611;InbreedingCoeff=0.1623;MQ=57.12;MQ0=0;MQRankSum=2.897;QD=31.50;ReadPosRankSum=0.605;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|11),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|9),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 886817 . C CATTTT 1195.05 . AC=14;AF=0.1296;AN=108;BaseQRankSum=-6.408;DP=215;FS=1.303;HRun=0;HaplotypeScore=40.0482;InbreedingCoeff=0.0654;MQ=57.03;MQ0=0;MQRankSum=-3.623;QD=23.43;ReadPosRankSum=-3.297;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|11),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|9),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 887162 . T C 158.95 . AC=3;AF=0.0273;AN=110;BaseQRankSum=0.867;DP=242;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1059;InbreedingCoeff=-0.1008;MQ=57.95;MQ0=0;MQRankSum=1.543;QD=10.60;ReadPosRankSum=-1.064;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|11),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|9),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 887265 . C A 101.07 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.874;DP=895;Dels=0.00;FS=2.947;HRun=0;HaplotypeScore=0.4283;InbreedingCoeff=-0.0106;MQ=59.14;MQ0=0;MQRankSum=2.148;QD=6.74;ReadPosRankSum=-0.250;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|11),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|9),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 887471 . G A 581.35 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.013;DP=3085;Dels=0.00;FS=3.856;HRun=1;HaplotypeScore=1.5844;InbreedingCoeff=-0.0084;MQ=59.14;MQ0=0;MQRankSum=3.944;QD=12.92;ReadPosRankSum=0.093;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|9),SYNONYMOUS_CODING(LOW|SILENT|Ctg/Ttg|L414|749|NOC2L|protein_coding|CODING|ENST00000327044|);CSQ=synonymous_variant|Ctg/Ttg|L|ENSG00000188976|NOC2L|ENST00000327044|11/19|||414/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|9/17||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 887537 . C T 1658.47 . AC=3;AF=0.0250;AN=120;BaseQRankSum=1.815;DP=2530;Dels=0.00;FS=6.149;HRun=0;HaplotypeScore=1.6593;InbreedingCoeff=-0.0258;MQ=59.28;MQ0=0;MQRankSum=2.238;QD=12.11;ReadPosRankSum=2.057;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|10),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|8);CSQ=intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 887560 . A C 70115.69 . AC=113;AF=0.9417;AN=120;BaseQRankSum=0.848;DP=2150;Dels=0.00;FS=10.744;HRun=1;HaplotypeScore=1.3105;InbreedingCoeff=0.2414;MQ=59.13;MQ0=0;MQRankSum=-0.778;QD=32.87;ReadPosRankSum=1.087;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|10),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|8);CSQ=intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 887651 . C T 665.1 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.019;DP=1257;Dels=0.00;FS=1.751;HRun=0;HaplotypeScore=0.9128;InbreedingCoeff=-0.0177;MQ=58.63;MQ0=0;MQRankSum=3.510;QD=17.50;ReadPosRankSum=2.127;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|10),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|8);CSQ=intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 887801 . A G 116758.48 . AC=115;AF=0.9583;AN=120;BaseQRankSum=4.615;DP=3778;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=2.1571;InbreedingCoeff=-0.0435;MQ=57.90;MQ0=0;MQRankSum=2.145;QD=30.90;ReadPosRankSum=1.213;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|8),SYNONYMOUS_CODING(LOW|SILENT|acT/acC|T394|749|NOC2L|protein_coding|CODING|ENST00000327044|);CSQ=synonymous_variant|acT/acC|T|ENSG00000188976|NOC2L|ENST00000327044|10/19|||394/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|8/17||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 888220 . G A 81.05 . AC=4;AF=0.044;AN=90;BaseQRankSum=-1.159;DP=137;Dels=0.00;FS=2.348;HRun=0;HaplotypeScore=0.1677;InbreedingCoeff=-0.0166;MQ=57.57;MQ0=0;MQRankSum=1.051;QD=7.37;ReadPosRankSum=1.796;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|9),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|7);CSQ=intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 888239 . C T 79.33 . AC=3;AF=0.034;AN=88;BaseQRankSum=0.324;DP=113;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0969;InbreedingCoeff=-0.0212;MQ=57.69;MQ0=0;MQRankSum=1.258;QD=13.22;ReadPosRankSum=-0.910;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|9),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|7);CSQ=intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 888639 . T C 94387.16 . AC=115;AF=0.9583;AN=120;BaseQRankSum=0.263;DP=3128;Dels=0.00;FS=2.314;HRun=1;HaplotypeScore=1.2974;InbreedingCoeff=-0.0435;MQ=54.95;MQ0=0;MQRankSum=0.465;QD=30.17;ReadPosRankSum=1.529;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|7),SYNONYMOUS_CODING(LOW|SILENT|gaA/gaG|E306|749|NOC2L|protein_coding|CODING|ENST00000327044|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,synonymous_variant|gaA/gaG|E|ENSG00000188976|NOC2L|ENST00000327044|9/19|||306/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|7/17||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 888659 . T C 80634.32 . AC=115;AF=0.9583;AN=120;BaseQRankSum=1.739;DP=2737;Dels=0.00;FS=2.276;HRun=1;HaplotypeScore=1.3379;InbreedingCoeff=-0.0436;MQ=54.78;MQ0=0;MQRankSum=-1.113;QD=29.46;ReadPosRankSum=4.132;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|7),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Atc/Gtc|I300V|749|NOC2L|protein_coding|CODING|ENST00000327044|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,missense_variant|Atc/Gtc|I/V|ENSG00000188976|NOC2L|ENST00000327044|9/19|benign(0)|tolerated(0.98)|300/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|7/17||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 889131 . A G 423.54 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-1.236;DP=1138;Dels=0.00;FS=7.564;HRun=1;HaplotypeScore=0.6841;InbreedingCoeff=-0.0300;MQ=57.89;MQ0=0;MQRankSum=0.858;QD=9.41;ReadPosRankSum=0.777;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|8),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|6);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 889158 . G C 40721.48 . AC=113;AF=0.9417;AN=120;BaseQRankSum=1.359;DP=1345;Dels=0.00;FS=0.512;HRun=1;HaplotypeScore=0.9432;InbreedingCoeff=0.2187;MQ=58.28;MQ0=0;MQRankSum=1.507;QD=30.55;ReadPosRankSum=0.613;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|8),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|6);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 889159 . A C 41244.53 . AC=113;AF=0.9417;AN=120;BaseQRankSum=1.309;DP=1361;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.8382;InbreedingCoeff=0.2198;MQ=58.26;MQ0=0;MQRankSum=1.567;QD=30.57;ReadPosRankSum=1.331;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|8),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|6);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 889238 . G A 1672.71 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-2.383;DP=1656;Dels=0.00;FS=10.199;HRun=0;HaplotypeScore=0.9421;InbreedingCoeff=-0.0479;MQ=58.37;MQ0=0;MQRankSum=0.456;QD=10.93;ReadPosRankSum=-1.085;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|6),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCg/gTg|A271V|749|NOC2L|protein_coding|CODING|ENST00000327044|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,missense_variant|gCg/gTg|A/V|ENSG00000188976|NOC2L|ENST00000327044|8/19|benign(0.034)|deleterious(0.04)|271/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|6/17||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 889638 . G C 14363.41 . AC=105;AF=0.9211;AN=114;BaseQRankSum=-0.922;DP=427;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3035;InbreedingCoeff=0.0466;MQ=57.19;MQ0=0;MQRankSum=0.153;QD=34.12;ReadPosRankSum=1.663;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|5),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|6);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|5/17||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 889713 . C A 5043.64 . AC=90;AF=0.918;AN=98;BaseQRankSum=-0.018;DP=155;Dels=0.00;FS=4.406;HRun=0;HaplotypeScore=0.0300;InbreedingCoeff=0.0572;MQ=56.80;MQ0=0;MQRankSum=1.329;QD=33.40;ReadPosRankSum=0.873;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|5),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|6);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|5/17||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 891021 . G A 7780.97 . AC=101;AF=0.9182;AN=110;BaseQRankSum=0.260;DP=242;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1735;InbreedingCoeff=-0.0202;MQ=58.75;MQ0=0;MQRankSum=-0.704;QD=33.11;ReadPosRankSum=0.580;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|6),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|6),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|4),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 891059 . C T 8049.27 . AC=70;AF=0.5932;AN=118;BaseQRankSum=0.106;DP=392;Dels=0.00;FS=5.538;HRun=2;HaplotypeScore=0.1844;InbreedingCoeff=0.1314;MQ=58.93;MQ0=0;MQRankSum=-2.097;QD=26.74;ReadPosRankSum=0.989;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|6),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|6),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|4),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 891401 . G A 2441.56 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.910;DP=6853;DS;Dels=0.00;FS=20.474;HRun=4;HaplotypeScore=2.8477;InbreedingCoeff=-0.0084;MQ=58.69;MQ0=0;MQRankSum=2.695;QD=14.45;ReadPosRankSum=2.367;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|5),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|5),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|3),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 891620 . C A 630.55 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.211;DP=3333;Dels=0.00;FS=17.146;HRun=0;HaplotypeScore=2.5646;InbreedingCoeff=-0.0084;MQ=59.15;MQ0=0;MQRankSum=0.979;QD=8.19;ReadPosRankSum=2.951;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|4),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|4),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 891898 . T C 58.0 . AC=2;AF=0.026;AN=78;BaseQRankSum=-1.208;DP=130;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0256;InbreedingCoeff=-0.1375;MQ=58.94;MQ0=0;MQRankSum=0.462;QD=4.46;ReadPosRankSum=-1.373;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|4),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|4),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 891945 . A G 1861.84 . AC=44;AF=0.550;AN=80;BaseQRankSum=-1.331;DP=117;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.1025;InbreedingCoeff=0.1989;MQ=59.45;MQ0=0;MQRankSum=-0.841;QD=24.82;ReadPosRankSum=0.357;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|4),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|4),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 892095 . C T 592.96 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.068;DP=1006;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5011;InbreedingCoeff=-0.0379;MQ=59.11;MQ0=0;MQRankSum=0.441;QD=14.82;ReadPosRankSum=1.641;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|4),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|4),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 892141 . C T 66.06 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-2.417;DP=1605;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.9164;InbreedingCoeff=-0.0175;MQ=59.28;MQ0=0;MQRankSum=0.188;QD=3.15;ReadPosRankSum=0.854;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|4),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|4),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 892237 . C A 5045.9 . AC=2;AF=0.0167;AN=120;BaseQRankSum=2.206;DP=4638;DS;Dels=0.00;FS=16.397;HRun=0;HaplotypeScore=2.4024;InbreedingCoeff=-0.0170;MQ=59.24;MQ0=0;MQRankSum=2.024;QD=16.22;ReadPosRankSum=-3.730;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|4),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|4),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 892460 . G C 4504.79 . AC=5;AF=0.0417;AN=120;BaseQRankSum=2.877;DP=3730;Dels=0.00;FS=1.037;HRun=0;HaplotypeScore=2.4668;InbreedingCoeff=-0.0435;MQ=59.20;MQ0=0;MQRankSum=1.411;QD=15.17;ReadPosRankSum=0.828;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|3),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|3),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|1),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 892470 . C T 571.4 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.526;DP=3581;Dels=0.00;FS=5.690;HRun=0;HaplotypeScore=4.5258;InbreedingCoeff=-0.0084;MQ=59.15;MQ0=0;MQRankSum=0.418;QD=11.90;ReadPosRankSum=0.317;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|3),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|3),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|1),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 892471 . G A 2720.48 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-7.069;DP=3602;Dels=0.00;FS=2.243;HRun=0;HaplotypeScore=2.7847;InbreedingCoeff=0.4828;MQ=59.14;MQ0=0;MQRankSum=-0.017;QD=13.95;ReadPosRankSum=-0.355;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|3),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|3),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|1),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 892724 . C T 85.66 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.766;DP=1274;Dels=0.00;FS=3.424;HRun=1;HaplotypeScore=0.8538;InbreedingCoeff=-0.0064;MQ=58.33;MQ0=0;MQRankSum=0.065;QD=14.28;ReadPosRankSum=-0.872;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|1),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|2),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|1/17||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 892745 . G A 33455.23 . AC=115;AF=0.9583;AN=120;BaseQRankSum=-1.159;DP=989;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3493;InbreedingCoeff=-0.0478;MQ=58.30;MQ0=0;MQRankSum=0.300;QD=33.83;ReadPosRankSum=-0.595;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|1),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|2),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|1/17||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 893719 . G A 126.7 . AC=10;AF=0.833;AN=12;BaseQRankSum=-0.198;DP=10;Dels=0.00;FS=3.680;HRun=0;HaplotypeScore=0.1223;MQ=57.66;MQ0=0;MQRankSum=-1.495;QD=21.12;ReadPosRankSum=0.198;EFF=EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|2),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|1),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|2),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000469563|2/2||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|1/17||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 894027 . G A 256.69 . AC=1;AF=0.012;AN=82;BaseQRankSum=-0.446;DP=427;Dels=0.00;FS=4.334;HRun=2;HaplotypeScore=0.2194;InbreedingCoeff=-0.0706;MQ=57.55;MQ0=0;MQRankSum=0.775;QD=18.34;ReadPosRankSum=-0.924;EFF=EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|2),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|2),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000469563|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 894330 . G A 79.5 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.248;DP=1450;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7814;InbreedingCoeff=-0.0087;MQ=58.82;MQ0=0;MQRankSum=0.499;QD=7.23;ReadPosRankSum=0.368;EFF=EXON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|2),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|2),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P53L|749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,missense_variant|cCg/cTg|P/L|ENSG00000188976|NOC2L|ENST00000327044|2/19|benign(0.001)|tolerated(0.41)|53/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000469563|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|2/7||||processed_transcript|1 +chr1 894519 . G A 127.69 . AC=2;AF=0.0167;AN=120;BaseQRankSum=0.782;DP=1334;Dels=0.00;FS=1.737;HRun=1;HaplotypeScore=0.3864;InbreedingCoeff=-0.0205;MQ=58.98;MQ0=0;MQRankSum=0.710;QD=6.72;ReadPosRankSum=0.813;EFF=INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|1),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|1),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|1),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 894573 . G A 31559.56 . AC=101;AF=0.8417;AN=120;BaseQRankSum=-0.317;DP=1219;Dels=0.00;FS=2.978;HRun=0;HaplotypeScore=0.8111;InbreedingCoeff=0.1756;MQ=58.45;MQ0=0;MQRankSum=-0.591;QD=27.47;ReadPosRankSum=-0.806;EFF=INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|1),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|1),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|1),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 894719 . C T 134.46 . AC=5;AF=0.0463;AN=108;BaseQRankSum=-1.930;DP=230;Dels=0.00;FS=6.502;HRun=0;HaplotypeScore=0.2397;InbreedingCoeff=0.1830;MQ=54.07;MQ0=0;MQRankSum=-0.173;QD=8.40;ReadPosRankSum=1.316;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 894890 . A AAGAC 433.26 . AC=10;AF=1.00;AN=10;DP=6;FS=0.000;HRun=0;HaplotypeScore=9.9355;MQ=29.00;MQ0=0;QD=72.21;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 895706 . G A 654.83 . AC=24;AF=0.857;AN=28;BaseQRankSum=-0.382;DP=28;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.1332;InbreedingCoeff=0.2034;MQ=55.74;MQ0=0;MQRankSum=1.582;QD=26.19;ReadPosRankSum=1.255;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 895755 . A AG 836.52 . AC=32;AF=0.941;AN=34;BaseQRankSum=-1.595;DP=39;FS=0.000;HRun=6;HaplotypeScore=8.1200;InbreedingCoeff=0.0511;MQ=54.87;MQ0=0;MQRankSum=1.595;QD=24.60;ReadPosRankSum=-1.456;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 896476 . A G 230.97 . AC=14;AF=1.000;AN=14;DP=11;Dels=0.00;FS=0.000;HRun=4;HaplotypeScore=0.0000;MQ=57.87;MQ0=0;QD=21.00;EFF=INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|1),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|1),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 896950 . C A 1565.88 . AC=3;AF=0.0273;AN=110;BaseQRankSum=-1.726;DP=1397;Dels=0.00;FS=5.899;HRun=0;HaplotypeScore=0.8800;InbreedingCoeff=-0.0557;MQ=59.13;MQ0=0;MQRankSum=0.483;QD=13.27;ReadPosRankSum=2.665;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|1),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|1),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|2),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|1/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 896977 . G C 992.92 . AC=1;AF=0.0086;AN=116;BaseQRankSum=3.226;DP=1654;Dels=0.00;FS=64.681;HRun=1;HaplotypeScore=1.1124;InbreedingCoeff=-0.0302;MQ=59.13;MQ0=0;MQRankSum=0.693;QD=19.09;ReadPosRankSum=-3.945;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|1),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|1),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|2),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|1/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 897325 . G C 125121.37 . AC=109;AF=0.9083;AN=120;BaseQRankSum=2.562;DP=4268;Dels=0.00;FS=6.072;HRun=1;HaplotypeScore=2.5759;InbreedingCoeff=0.0992;MQ=58.65;MQ0=0;MQRankSum=-0.923;QD=29.72;ReadPosRankSum=-2.769;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),SYNONYMOUS_CODING(LOW|SILENT|gcG/gcC|A203|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),UTR_5_PRIME(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|3);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,synonymous_variant|gcG/gcC|A|ENSG00000187961|KLHL17|ENST00000338591|4/12|||203/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 897337 . C T 1277.99 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.453;DP=4287;Dels=0.00;FS=8.422;HRun=0;HaplotypeScore=2.3521;InbreedingCoeff=-0.0084;MQ=58.59;MQ0=0;MQRankSum=0.646;QD=14.36;ReadPosRankSum=0.556;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),START_GAINED(LOW||||412|KLHL17|protein_coding|CODING|ENST00000455747|3),SYNONYMOUS_CODING(LOW|SILENT|agC/agT|S207|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,synonymous_variant|agC/agT|S|ENSG00000187961|KLHL17|ENST00000338591|4/12|||207/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 897460 . A C 4476.36 . AC=24;AF=0.2069;AN=116;BaseQRankSum=-6.649;DP=4306;DS;Dels=0.00;FS=1182.881;HRun=6;HaplotypeScore=18.5719;InbreedingCoeff=-0.2955;MQ=53.12;MQ0=0;MQRankSum=-6.585;QD=1.27;ReadPosRankSum=-5.387;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|3),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|4),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 897465 . A C 207.3 . AC=8;AF=0.0678;AN=118;BaseQRankSum=-6.006;DP=4323;DS;Dels=0.00;FS=207.986;HRun=4;HaplotypeScore=15.9325;InbreedingCoeff=-0.0865;MQ=53.13;MQ0=0;MQRankSum=-13.595;QD=0.15;ReadPosRankSum=-11.475;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|3),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|4),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 897470 . A C 60.04 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-10.911;DP=4336;DS;Dels=0.00;FS=267.922;HRun=4;HaplotypeScore=16.3920;InbreedingCoeff=-0.0465;MQ=53.18;MQ0=0;MQRankSum=-11.192;QD=0.22;ReadPosRankSum=-11.844;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|3),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|4),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 897564 . T C 105645.82 . AC=111;AF=0.9407;AN=118;BaseQRankSum=-0.165;DP=3668;DS;Dels=0.00;FS=4.440;HRun=1;HaplotypeScore=1.8075;InbreedingCoeff=0.1915;MQ=54.46;MQ0=0;MQRankSum=3.570;QD=29.65;ReadPosRankSum=-1.056;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|3),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|4),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 897641 . C T 42.61 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.313;DP=2743;DS;Dels=0.00;FS=9.036;HRun=0;HaplotypeScore=1.2616;InbreedingCoeff=-0.0088;MQ=55.91;MQ0=0;MQRankSum=0.432;QD=3.87;ReadPosRankSum=0.049;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|3),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|4),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 897730 . C T 1850.25 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.726;DP=3106;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=3.1479;InbreedingCoeff=-0.0256;MQ=58.41;MQ0=0;MQRankSum=-0.353;QD=12.94;ReadPosRankSum=-0.223;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|3),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|4),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 897738 . C T 5799.05 . AC=8;AF=0.0667;AN=120;BaseQRankSum=1.153;DP=3157;Dels=0.00;FS=6.498;HRun=2;HaplotypeScore=2.6795;InbreedingCoeff=0.1964;MQ=58.52;MQ0=0;MQRankSum=1.044;QD=16.11;ReadPosRankSum=-1.772;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),SYNONYMOUS_CODING(LOW|SILENT|Ctg/Ttg|L239|642|KLHL17|protein_coding|CODING|ENST00000338591|),SYNONYMOUS_CODING(LOW|SILENT|Ctg/Ttg|L9|412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,synonymous_variant|Ctg/Ttg|L|ENSG00000187961|KLHL17|ENST00000338591|5/12|||239/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 897879 . C T 1100.72 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-0.411;DP=1081;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7665;InbreedingCoeff=-0.0357;MQ=58.62;MQ0=0;MQRankSum=0.745;QD=12.80;ReadPosRankSum=2.064;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|4),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|5),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 898068 . G C 79.28 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.132;DP=1385;Dels=0.00;FS=2.990;HRun=1;HaplotypeScore=0.9318;InbreedingCoeff=0.8872;MQ=57.94;MQ0=0;MQRankSum=0.935;QD=19.82;ReadPosRankSum=-0.735;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|4),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|5),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 898323 . T C 52014.58 . AC=115;AF=0.9583;AN=120;BaseQRankSum=-0.884;DP=1603;Dels=0.00;FS=3.229;HRun=1;HaplotypeScore=0.9054;InbreedingCoeff=-0.0448;MQ=58.52;MQ0=0;MQRankSum=-0.998;QD=32.45;ReadPosRankSum=2.785;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|5),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|6),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=intron_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 898467 . C T 1510.84 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-0.034;DP=1255;Dels=0.00;FS=4.507;HRun=0;HaplotypeScore=0.8357;InbreedingCoeff=-0.0475;MQ=58.85;MQ0=0;MQRankSum=-1.026;QD=13.49;ReadPosRankSum=-1.131;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|5),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|6),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=synonymous_variant&NMD_transcript_variant|ccC/ccT|P|ENSG00000187961|KLHL17|ENST00000466300|2/6|||83/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 898603 . C G 116.84 . AC=8;AF=0.0667;AN=120;BaseQRankSum=-8.523;DP=3456;Dels=0.00;FS=28.932;HRun=2;HaplotypeScore=4.3555;InbreedingCoeff=-0.0746;MQ=57.81;MQ0=0;MQRankSum=-3.388;QD=0.70;ReadPosRankSum=-5.743;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCg/gGg|A156G|412|KLHL17|protein_coding|CODING|ENST00000455747|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCg/gGg|A386G|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=missense_variant&NMD_transcript_variant|Cgg/Ggg|R/G|ENSG00000187961|KLHL17|ENST00000466300|2/6|unknown(0)|deleterious(0)|129/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,missense_variant|gCg/gGg|A/G|ENSG00000187961|KLHL17|ENST00000338591|7/12|possibly_damaging(0.8)|tolerated(0.13)|386/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 898604 . G A 684.72 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.572;DP=3527;Dels=0.00;FS=8.272;HRun=0;HaplotypeScore=4.4131;InbreedingCoeff=-0.0111;MQ=57.83;MQ0=0;MQRankSum=0.825;QD=9.92;ReadPosRankSum=-0.003;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),SYNONYMOUS_CODING(LOW|SILENT|gcG/gcA|A156|412|KLHL17|protein_coding|CODING|ENST00000455747|),SYNONYMOUS_CODING(LOW|SILENT|gcG/gcA|A386|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=missense_variant&NMD_transcript_variant|cGg/cAg|R/Q|ENSG00000187961|KLHL17|ENST00000466300|2/6|unknown(0)|deleterious(0)|129/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,synonymous_variant|gcG/gcA|A|ENSG00000187961|KLHL17|ENST00000338591|7/12|||386/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 898893 . G C 345.84 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.109;DP=2327;Dels=0.00;FS=1.347;HRun=0;HaplotypeScore=1.8649;InbreedingCoeff=-0.0084;MQ=58.71;MQ0=0;MQRankSum=0.616;QD=12.81;ReadPosRankSum=0.720;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|7),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|8),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|);CSQ=intron_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 898934 . G A 47.08 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.111;DP=1477;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6015;InbreedingCoeff=-0.0091;MQ=58.84;MQ0=0;MQRankSum=0.087;QD=5.89;ReadPosRankSum=-1.045;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|1),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|7),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|8),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|);CSQ=intron_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000481067|1/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 899000 . C T 654.34 . AC=8;AF=0.0667;AN=120;BaseQRankSum=-0.932;DP=651;Dels=0.00;FS=1.229;HRun=0;HaplotypeScore=0.5946;InbreedingCoeff=0.3177;MQ=58.35;MQ0=0;MQRankSum=0.454;QD=16.36;ReadPosRankSum=0.885;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|1),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|7),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|8),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|);CSQ=intron_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000481067|1/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 899318 . C T 428.77 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.231;DP=2100;Dels=0.00;FS=29.933;HRun=0;HaplotypeScore=1.6653;InbreedingCoeff=-0.0087;MQ=58.94;MQ0=0;MQRankSum=-0.016;QD=10.99;ReadPosRankSum=-1.614;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|1),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P228|412|KLHL17|protein_coding|CODING|ENST00000455747|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P458|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|);CSQ=3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300|4/6|||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,synonymous_variant|ccC/ccT|P|ENSG00000187961|KLHL17|ENST00000338591|9/12|||458/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000481067|1/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 899374 . G A 298.41 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.204;DP=2588;Dels=0.00;FS=1.169;HRun=1;HaplotypeScore=1.4609;InbreedingCoeff=-0.0084;MQ=58.81;MQ0=0;MQRankSum=0.082;QD=10.29;ReadPosRankSum=2.022;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGa/cAa|R247Q|412|KLHL17|protein_coding|CODING|ENST00000455747|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGa/cAa|R477Q|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|);CSQ=3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300|4/6|||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,missense_variant|cGa/cAa|R/Q|ENSG00000187961|KLHL17|ENST00000338591|9/12|possibly_damaging(0.886)|deleterious(0.03)|477/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000481067|1/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 899515 . C T 554.46 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.826;DP=4376;Dels=0.00;FS=0.766;HRun=0;HaplotypeScore=2.1786;InbreedingCoeff=-0.0084;MQ=58.98;MQ0=0;MQRankSum=-0.199;QD=13.86;ReadPosRankSum=2.919;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|2),SYNONYMOUS_CODING(LOW|SILENT|taC/taT|Y261|412|KLHL17|protein_coding|CODING|ENST00000455747|),SYNONYMOUS_CODING(LOW|SILENT|taC/taT|Y491|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|);CSQ=3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300|5/6|||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,synonymous_variant|taC/taT|Y|ENSG00000187961|KLHL17|ENST00000338591|10/12|||491/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000481067|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 899648 . C T 506.51 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.603;DP=2206;Dels=0.00;FS=1.040;HRun=0;HaplotypeScore=2.2848;InbreedingCoeff=-0.0084;MQ=57.02;MQ0=0;MQRankSum=0.240;QD=14.90;ReadPosRankSum=1.277;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|9),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|10),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|);CSQ=intron_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 +chr1 899824 . C T 386.57 . AC=2;AF=0.0169;AN=118;BaseQRankSum=-1.360;DP=1027;Dels=0.00;FS=0.938;HRun=0;HaplotypeScore=0.7940;InbreedingCoeff=-0.0327;MQ=52.55;MQ0=0;MQRankSum=2.078;QD=10.17;ReadPosRankSum=-2.959;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N308|412|KLHL17|protein_coding|CODING|ENST00000455747|),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N538|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300|6/6|||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,synonymous_variant|aaC/aaT|N|ENSG00000187961|KLHL17|ENST00000338591|11/12|||538/642|protein_coding|1 +chr1 899926 . GC G 146.78 . AC=3;AF=0.0273;AN=110;BaseQRankSum=-1.149;DP=401;FS=8.501;HRun=1;HaplotypeScore=98.5276;InbreedingCoeff=-0.0768;MQ=46.11;MQ0=0;MQRankSum=-4.025;QD=4.32;ReadPosRankSum=-1.794;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 899928 . G C 10304.35 . AC=95;AF=0.8636;AN=110;BaseQRankSum=-2.647;DP=394;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.9435;InbreedingCoeff=-0.0052;MQ=45.90;MQ0=0;MQRankSum=-1.207;QD=26.35;ReadPosRankSum=1.974;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 899937 . G T 7323.3 . AC=86;AF=0.860;AN=100;BaseQRankSum=-1.886;DP=301;Dels=0.00;FS=4.882;HRun=0;HaplotypeScore=1.0717;InbreedingCoeff=0.2201;MQ=45.04;MQ0=0;MQRankSum=-1.460;QD=25.61;ReadPosRankSum=2.493;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 899938 . G C 7723.52 . AC=86;AF=0.860;AN=100;BaseQRankSum=-1.269;DP=292;Dels=0.00;FS=6.709;HRun=1;HaplotypeScore=0.9273;InbreedingCoeff=0.2346;MQ=45.14;MQ0=0;MQRankSum=-2.046;QD=27.78;ReadPosRankSum=2.689;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 899942 . G A 9223.42 . AC=87;AF=0.8529;AN=102;BaseQRankSum=-0.795;DP=333;Dels=0.00;FS=4.035;HRun=0;HaplotypeScore=0.7409;InbreedingCoeff=0.1074;MQ=43.28;MQ0=0;MQRankSum=-1.361;QD=28.38;ReadPosRankSum=1.851;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 899943 . G C 99.41 . AC=3;AF=0.0294;AN=102;BaseQRankSum=-1.668;DP=334;Dels=0.00;FS=4.946;HRun=0;HaplotypeScore=0.6513;InbreedingCoeff=-0.0759;MQ=42.96;MQ0=0;MQRankSum=-3.741;QD=2.76;ReadPosRankSum=-1.346;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 899959 . G GC 686.45 . AC=19;AF=0.190;AN=100;BaseQRankSum=-3.215;DP=298;FS=12.605;HRun=0;HaplotypeScore=100.2656;InbreedingCoeff=-0.1846;MQ=45.28;MQ0=0;MQRankSum=-5.322;QD=4.40;ReadPosRankSum=0.742;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 899960 . T C 416.06 . AC=19;AF=0.190;AN=100;BaseQRankSum=3.474;DP=298;Dels=0.00;FS=12.762;HRun=0;HaplotypeScore=0.3615;InbreedingCoeff=-0.1710;MQ=45.28;MQ0=0;MQRankSum=-6.659;QD=2.87;ReadPosRankSum=-1.194;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 899974 . G A 175.25 . AC=17;AF=0.170;AN=100;BaseQRankSum=1.366;DP=246;Dels=0.00;FS=8.615;HRun=0;HaplotypeScore=0.2560;InbreedingCoeff=-0.1500;MQ=45.05;MQ0=0;MQRankSum=-5.035;QD=1.79;ReadPosRankSum=-2.296;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 899975 . C G 161.84 . AC=18;AF=0.180;AN=100;BaseQRankSum=1.074;DP=239;Dels=0.00;FS=8.632;HRun=1;HaplotypeScore=0.2414;InbreedingCoeff=-0.1746;MQ=44.67;MQ0=0;MQRankSum=-6.140;QD=1.54;ReadPosRankSum=-2.132;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 899987 . GGATGT G 805.24 . AC=10;AF=0.102;AN=98;BaseQRankSum=-2.007;DP=183;FS=4.301;HRun=0;HaplotypeScore=71.0169;InbreedingCoeff=0.1259;MQ=41.55;MQ0=0;MQRankSum=-2.599;QD=19.64;ReadPosRankSum=-2.063;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 899998 . G C 172.67 . AC=3;AF=0.0288;AN=104;BaseQRankSum=-3.045;DP=165;Dels=0.01;FS=6.898;HRun=1;HaplotypeScore=3.2446;InbreedingCoeff=-0.1274;MQ=38.58;MQ0=0;MQRankSum=-2.770;QD=7.85;ReadPosRankSum=2.697;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900000 . G GAA 4516.13 . AC=82;AF=0.8039;AN=102;BaseQRankSum=-0.011;DP=158;FS=8.933;HRun=0;HaplotypeScore=53.7512;InbreedingCoeff=0.0958;MQ=37.58;MQ0=0;MQRankSum=-2.778;QD=29.91;ReadPosRankSum=0.180;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900001 . G A 1769.07 . AC=55;AF=0.561;AN=98;BaseQRankSum=-1.838;DP=158;Dels=0.00;FS=12.659;HRun=1;HaplotypeScore=4.3165;InbreedingCoeff=0.0703;MQ=37.58;MQ0=0;MQRankSum=-1.578;QD=15.38;ReadPosRankSum=-0.873;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900009 . C G 1460.59 . AC=51;AF=0.510;AN=100;BaseQRankSum=1.557;DP=154;Dels=0.00;FS=5.254;HRun=6;HaplotypeScore=3.7869;InbreedingCoeff=0.1584;MQ=37.06;MQ0=0;MQRankSum=-2.397;QD=14.75;ReadPosRankSum=1.027;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900009 . CG C 3423.69 . AC=75;AF=0.765;AN=98;BaseQRankSum=-1.591;DP=149;FS=3.362;HRun=6;HaplotypeScore=53.0225;InbreedingCoeff=-0.0140;MQ=37.05;MQ0=0;MQRankSum=0.377;QD=24.45;ReadPosRankSum=3.509;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900010 . G GC 2274.92 . AC=67;AF=0.684;AN=98;BaseQRankSum=-2.660;DP=149;FS=0.000;HRun=1;HaplotypeScore=58.6576;InbreedingCoeff=0.1712;MQ=37.05;MQ0=0;MQRankSum=0.765;QD=18.35;ReadPosRankSum=2.099;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900012 . G A 52.89 . AC=9;AF=0.092;AN=98;BaseQRankSum=-1.584;DP=144;Dels=0.00;FS=9.518;HRun=0;HaplotypeScore=1.9499;InbreedingCoeff=-0.1191;MQ=37.30;MQ0=0;MQRankSum=-1.274;QD=1.56;ReadPosRankSum=-3.899;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900018 . G A 202.57 . AC=3;AF=0.033;AN=90;BaseQRankSum=-0.005;DP=138;Dels=0.00;FS=14.921;HRun=0;HaplotypeScore=2.7642;InbreedingCoeff=-0.1390;MQ=37.35;MQ0=0;MQRankSum=-2.631;QD=11.25;ReadPosRankSum=-0.202;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900025 . GGATGT G 524.76 . AC=16;AF=0.174;AN=92;BaseQRankSum=3.838;DP=133;FS=8.461;HRun=0;HaplotypeScore=57.1337;InbreedingCoeff=-0.0789;MQ=37.07;MQ0=0;MQRankSum=-2.025;QD=12.49;ReadPosRankSum=0.267;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900036 . C G 56.0 . AC=8;AF=0.095;AN=84;BaseQRankSum=-0.360;DP=115;Dels=0.02;FS=4.181;HRun=3;HaplotypeScore=2.4570;InbreedingCoeff=0.0759;MQ=37.96;MQ0=0;MQRankSum=-2.183;QD=3.73;ReadPosRankSum=1.383;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900045 . G T 197.92 . AC=12;AF=0.158;AN=76;BaseQRankSum=-0.033;DP=97;Dels=0.00;FS=9.602;HRun=0;HaplotypeScore=0.8494;InbreedingCoeff=-0.1283;MQ=36.39;MQ0=0;MQRankSum=-3.050;QD=6.60;ReadPosRankSum=0.794;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900046 . G C 221.18 . AC=14;AF=0.194;AN=72;BaseQRankSum=0.606;DP=95;Dels=0.00;FS=15.119;HRun=1;HaplotypeScore=0.8152;InbreedingCoeff=-0.0844;MQ=36.53;MQ0=0;MQRankSum=-3.254;QD=6.91;ReadPosRankSum=0.931;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900047 . CG C 209.92 . AC=15;AF=0.208;AN=72;BaseQRankSum=1.707;DP=88;FS=0.000;HRun=6;HaplotypeScore=49.2935;InbreedingCoeff=-0.1925;MQ=36.21;MQ0=0;MQRankSum=-2.511;QD=4.47;ReadPosRankSum=0.230;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900050 . G A 240.82 . AC=14;AF=0.226;AN=62;BaseQRankSum=1.097;DP=84;Dels=0.00;FS=15.435;HRun=0;HaplotypeScore=0.6581;InbreedingCoeff=-0.0898;MQ=34.67;MQ0=0;MQRankSum=-1.036;QD=8.03;ReadPosRankSum=0.391;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900065 . A C 247.12 . AC=17;AF=0.304;AN=56;BaseQRankSum=1.516;DP=63;Dels=0.00;FS=9.830;HRun=0;HaplotypeScore=2.1490;InbreedingCoeff=-0.0549;MQ=32.56;MQ0=0;MQRankSum=-0.918;QD=7.06;ReadPosRankSum=2.224;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900068 . TGCTGCC T 518.39 . AC=13;AF=0.217;AN=60;BaseQRankSum=4.713;DP=59;FS=14.526;HRun=0;HaplotypeScore=44.2811;InbreedingCoeff=0.0509;MQ=31.33;MQ0=0;MQRankSum=-0.386;QD=28.80;ReadPosRankSum=4.120;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900083 . G T 196.8 . AC=12;AF=0.286;AN=42;BaseQRankSum=-2.303;DP=46;Dels=0.00;FS=9.918;HRun=0;HaplotypeScore=1.2000;InbreedingCoeff=0.0257;MQ=29.00;MQ0=0;MQRankSum=0.317;QD=10.36;ReadPosRankSum=1.627;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900084 . G C 236.51 . AC=14;AF=0.350;AN=40;BaseQRankSum=-0.904;DP=46;Dels=0.00;FS=12.035;HRun=1;HaplotypeScore=1.2233;InbreedingCoeff=0.0591;MQ=29.00;MQ0=0;MQRankSum=-0.215;QD=11.83;ReadPosRankSum=1.420;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900085 . C G 54.45 . AC=7;AF=0.175;AN=40;BaseQRankSum=1.312;DP=45;Dels=0.00;FS=0.000;HRun=6;HaplotypeScore=0.9242;InbreedingCoeff=0.1696;MQ=29.00;MQ0=0;MQRankSum=0.641;QD=7.78;ReadPosRankSum=-1.373;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900086 . G GC 66.18 . AC=9;AF=0.265;AN=34;BaseQRankSum=-0.405;DP=42;FS=3.909;HRun=1;HaplotypeScore=46.6346;InbreedingCoeff=0.0729;MQ=29.00;MQ0=0;MQRankSum=0.231;QD=4.41;ReadPosRankSum=-0.925;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900089 . G C 230.72 . AC=14;AF=0.500;AN=28;BaseQRankSum=0.692;DP=39;Dels=0.00;FS=10.780;HRun=0;HaplotypeScore=0.8484;InbreedingCoeff=0.2201;MQ=29.00;MQ0=0;MQRankSum=0.165;QD=14.42;ReadPosRankSum=0.495;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900103 . A C 245.84 . AC=13;AF=0.650;AN=20;BaseQRankSum=2.098;DP=29;Dels=0.00;FS=4.393;HRun=0;HaplotypeScore=1.1682;InbreedingCoeff=-0.0533;MQ=29.00;MQ0=0;MQRankSum=2.098;QD=15.37;ReadPosRankSum=0.146;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900112 . C G 149.58 . AC=10;AF=0.556;AN=18;BaseQRankSum=1.027;DP=24;Dels=0.04;FS=2.276;HRun=3;HaplotypeScore=0.9865;MQ=29.00;MQ0=0;MQRankSum=1.027;QD=12.47;ReadPosRankSum=0.201;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900115 . G A 35.09 . AC=5;AF=0.227;AN=22;BaseQRankSum=2.382;DP=23;Dels=0.00;FS=13.570;HRun=1;HaplotypeScore=1.7631;InbreedingCoeff=0.1307;MQ=29.00;MQ0=0;MQRankSum=-0.361;QD=7.02;ReadPosRankSum=-1.083;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900123 . C G 41.39 . AC=5;AF=0.357;AN=14;BaseQRankSum=1.931;DP=20;Dels=0.00;FS=14.771;HRun=6;HaplotypeScore=1.5461;MQ=29.00;MQ0=0;MQRankSum=0.000;QD=5.17;ReadPosRankSum=-0.382;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900123 . CG C 239.86 . AC=12;AF=0.600;AN=20;BaseQRankSum=0.715;DP=20;FS=2.363;HRun=6;HaplotypeScore=37.1615;InbreedingCoeff=0.0592;MQ=29.00;MQ0=0;MQRankSum=-0.965;QD=17.13;ReadPosRankSum=0.627;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900161 . C G 95.96 . AC=3;AF=0.041;AN=74;BaseQRankSum=-0.808;DP=152;Dels=0.00;FS=0.000;HRun=6;HaplotypeScore=0.5011;InbreedingCoeff=-0.1315;MQ=55.56;MQ0=0;MQRankSum=1.024;QD=4.17;ReadPosRankSum=-2.738;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900188 . C G 109.47 . AC=2;AF=0.021;AN=96;BaseQRankSum=0.425;DP=319;Dels=0.00;FS=0.000;HRun=3;HaplotypeScore=0.5571;InbreedingCoeff=-0.0765;MQ=55.58;MQ0=0;MQRankSum=-4.308;QD=9.95;ReadPosRankSum=-1.562;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900191 . G A 78.75 . AC=2;AF=0.020;AN=98;BaseQRankSum=0.169;DP=359;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.6619;InbreedingCoeff=-0.0713;MQ=55.88;MQ0=0;MQRankSum=-3.973;QD=7.16;ReadPosRankSum=-0.841;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900199 . C G 122.69 . AC=2;AF=0.0196;AN=102;BaseQRankSum=-0.625;DP=419;Dels=0.00;FS=5.991;HRun=6;HaplotypeScore=0.9712;InbreedingCoeff=-0.0705;MQ=55.82;MQ0=0;MQRankSum=-4.226;QD=11.15;ReadPosRankSum=-0.138;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900200 . G GC 138.36 . AC=2;AF=0.0192;AN=104;BaseQRankSum=4.004;DP=447;FS=5.745;HRun=1;HaplotypeScore=63.8129;InbreedingCoeff=-0.0683;MQ=55.87;MQ0=0;MQRankSum=-4.054;QD=10.64;ReadPosRankSum=-0.688;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900205 . T C 3848.55 . AC=28;AF=0.2642;AN=106;BaseQRankSum=3.976;DP=500;Dels=0.00;FS=4.311;HRun=2;HaplotypeScore=0.8450;InbreedingCoeff=-0.0127;MQ=56.04;MQ0=0;MQRankSum=2.371;QD=15.21;ReadPosRankSum=0.624;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900208 . G A 196.8 . AC=3;AF=0.0278;AN=108;BaseQRankSum=-1.850;DP=545;Dels=0.00;FS=3.034;HRun=0;HaplotypeScore=0.9217;InbreedingCoeff=-0.0711;MQ=55.89;MQ0=0;MQRankSum=-1.612;QD=8.56;ReadPosRankSum=-1.195;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900252 . G A 2492.99 . AC=7;AF=0.0593;AN=118;BaseQRankSum=0.859;DP=1112;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.6311;InbreedingCoeff=-0.0857;MQ=55.70;MQ0=0;MQRankSum=2.953;QD=13.33;ReadPosRankSum=-0.555;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900285 . C T 49972.75 . AC=105;AF=0.9052;AN=116;BaseQRankSum=-3.461;DP=1713;Dels=0.00;FS=2.048;HRun=0;HaplotypeScore=2.6694;InbreedingCoeff=0.0387;MQ=55.27;MQ0=0;MQRankSum=-1.138;QD=29.80;ReadPosRankSum=-0.012;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900286 . A G 53795.91 . AC=111;AF=0.9569;AN=116;BaseQRankSum=-1.271;DP=1744;Dels=0.00;FS=6.893;HRun=1;HaplotypeScore=2.7132;InbreedingCoeff=-0.0679;MQ=55.34;MQ0=0;MQRankSum=0.681;QD=30.85;ReadPosRankSum=0.607;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900298 . C G 1312.84 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-2.430;DP=2069;Dels=0.00;FS=2.014;HRun=1;HaplotypeScore=1.8253;InbreedingCoeff=0.1410;MQ=55.23;MQ0=0;MQRankSum=1.938;QD=10.67;ReadPosRankSum=-0.720;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900319 . G A 830.69 . AC=3;AF=0.0250;AN=120;BaseQRankSum=0.864;DP=2658;Dels=0.00;FS=5.182;HRun=0;HaplotypeScore=1.8883;InbreedingCoeff=-0.0417;MQ=56.04;MQ0=0;MQRankSum=2.734;QD=10.93;ReadPosRankSum=1.705;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 900397 . C T 2009.84 . AC=2;AF=0.0167;AN=120;BaseQRankSum=1.145;DP=5436;DS;Dels=0.00;FS=0.454;HRun=0;HaplotypeScore=4.5718;InbreedingCoeff=-0.0195;MQ=57.26;MQ0=0;MQRankSum=1.619;QD=12.72;ReadPosRankSum=-0.157;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N355|412|KLHL17|protein_coding|CODING|ENST00000455747|),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N585|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,synonymous_variant|aaC/aaT|N|ENSG00000187961|KLHL17|ENST00000338591|12/12|||585/642|protein_coding|1 +chr1 900505 . G C 36708.31 . AC=34;AF=0.2881;AN=118;BaseQRankSum=3.779;DP=6012;DS;Dels=0.00;FS=16.118;HRun=1;HaplotypeScore=4.6415;InbreedingCoeff=-0.1592;MQ=56.94;MQ0=0;MQRankSum=2.324;QD=13.04;ReadPosRankSum=-1.518;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),SYNONYMOUS_CODING(LOW|SILENT|gtG/gtC|V391|412|KLHL17|protein_coding|CODING|ENST00000455747|),SYNONYMOUS_CODING(LOW|SILENT|gtG/gtC|V621|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,synonymous_variant|gtG/gtC|V|ENSG00000187961|KLHL17|ENST00000338591|12/12|||621/642|protein_coding|1 +chr1 900560 . A C 336.55 . AC=11;AF=0.0917;AN=120;BaseQRankSum=-16.919;DP=3961;DS;Dels=0.00;FS=94.082;HRun=2;HaplotypeScore=2.4690;InbreedingCoeff=-0.1027;MQ=56.02;MQ0=0;MQRankSum=-0.133;QD=2.37;ReadPosRankSum=-4.673;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Acc/Ccc|T410P|412|KLHL17|protein_coding|CODING|ENST00000455747|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Acc/Ccc|T640P|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,missense_variant|Acc/Ccc|T/P|ENSG00000187961|KLHL17|ENST00000338591|12/12|unknown(0)|deleterious(0.02)|640/642|protein_coding|1 +chr1 900717 . CTTAT C 9074.2 . AC=69;AF=0.6053;AN=114;BaseQRankSum=10.277;DP=412;FS=7.468;HRun=0;HaplotypeScore=48.3915;InbreedingCoeff=0.0298;MQ=48.25;MQ0=0;MQRankSum=-9.614;QD=26.85;ReadPosRankSum=3.811;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UTR_3_PRIME(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|11),UTR_3_PRIME(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|12);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,3_prime_UTR_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591|12/12|||-/642|protein_coding|1 +chr1 900723 . T G 285.98 . AC=14;AF=0.1228;AN=114;BaseQRankSum=-3.773;DP=321;Dels=0.00;FS=2.147;HRun=0;HaplotypeScore=11.3195;InbreedingCoeff=-0.1788;MQ=46.06;MQ0=0;MQRankSum=4.705;QD=2.95;ReadPosRankSum=-6.059;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UTR_3_PRIME(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|11),UTR_3_PRIME(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|12);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187961|KLHL17|ENST00000338591|12/12|||-/642|protein_coding|1 +chr1 900730 . G A 7842.1 . AC=100;AF=0.8621;AN=116;BaseQRankSum=-0.675;DP=275;Dels=0.00;FS=1.534;HRun=0;HaplotypeScore=5.4770;InbreedingCoeff=0.2758;MQ=43.76;MQ0=0;MQRankSum=-4.770;QD=29.93;ReadPosRankSum=-1.794;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UTR_3_PRIME(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|11),UTR_3_PRIME(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|12);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187961|KLHL17|ENST00000338591|12/12|||-/642|protein_coding|1 +chr1 900972 . T G 837.46 . AC=38;AF=0.950;AN=40;BaseQRankSum=1.091;DP=28;Dels=0.00;FS=5.863;HRun=1;HaplotypeScore=0.0000;InbreedingCoeff=-0.0011;MQ=56.62;MQ0=0;MQRankSum=1.091;QD=32.21;ReadPosRankSum=1.348;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UTR_3_PRIME(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|11),UTR_3_PRIME(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|12);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187961|KLHL17|ENST00000338591|12/12|||-/642|protein_coding|1 +chr1 901023 . T C 668.0 . AC=30;AF=0.882;AN=34;BaseQRankSum=0.039;DP=25;Dels=0.00;FS=13.318;HRun=0;HaplotypeScore=0.1562;InbreedingCoeff=-0.0502;MQ=59.07;MQ0=0;MQRankSum=-1.123;QD=29.04;ReadPosRankSum=-2.053;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UTR_3_PRIME(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|11),UTR_3_PRIME(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|12);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187961|KLHL17|ENST00000338591|12/12|||-/642|protein_coding|1 +chr1 901046 . ACC A 367.24 . AC=14;AF=0.368;AN=38;BaseQRankSum=3.349;DP=34;FS=3.319;HRun=4;HaplotypeScore=8.5629;InbreedingCoeff=0.2418;MQ=57.94;MQ0=0;MQRankSum=-0.562;QD=26.23;ReadPosRankSum=0.832;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UTR_3_PRIME(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|11),UTR_3_PRIME(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|12);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,3_prime_UTR_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591|12/12|||-/642|protein_coding|1 +chr1 901460 . C T 32.18 . AC=2;AF=0.045;AN=44;BaseQRankSum=0.046;DP=29;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0879;InbreedingCoeff=-0.0076;MQ=59.20;MQ0=0;MQRankSum=0.231;QD=16.09;ReadPosRankSum=-1.435;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 901559 . G A 1182.94 . AC=46;AF=0.920;AN=50;BaseQRankSum=0.433;DP=38;Dels=0.00;FS=2.501;HRun=0;HaplotypeScore=0.1286;InbreedingCoeff=-0.0789;MQ=56.89;MQ0=0;MQRankSum=-0.974;QD=31.97;ReadPosRankSum=-0.704;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 901607 . C G 1775.67 . AC=57;AF=0.983;AN=58;BaseQRankSum=-0.817;DP=56;Dels=0.00;FS=0.000;HRun=6;HaplotypeScore=0.0000;InbreedingCoeff=-0.1683;MQ=56.62;MQ0=0;MQRankSum=-0.443;QD=32.28;ReadPosRankSum=-0.303;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 901652 . A G 2979.53 . AC=72;AF=1.000;AN=72;DP=87;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0518;InbreedingCoeff=-0.1163;MQ=57.30;MQ0=0;QD=34.65;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 901806 . G C 1993.1 . AC=13;AF=0.1204;AN=108;BaseQRankSum=0.578;DP=624;Dels=0.00;FS=2.492;HRun=3;HaplotypeScore=0.2501;InbreedingCoeff=0.2481;MQ=58.77;MQ0=0;MQRankSum=1.452;QD=19.16;ReadPosRankSum=0.786;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 901919 . A C 43.36 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-7.616;DP=1450;Dels=0.00;FS=55.854;HRun=1;HaplotypeScore=0.9087;InbreedingCoeff=-0.0627;MQ=58.76;MQ0=0;MQRankSum=-1.305;QD=0.46;ReadPosRankSum=-4.128;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAc/aCc|N3T|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAc/aCc|N3T|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAc/aCc|N3T|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,missense_variant|aAc/aCc|N/T|ENSG00000187583|PLEKHN1|ENST00000379409|1/15|possibly_damaging(0.471)|deleterious(0.01)|3/663|protein_coding|1,missense_variant|aAc/aCc|N/T|ENSG00000187583|PLEKHN1|ENST00000379407|1/15|benign(0.331)|deleterious(0.01)|3/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,missense_variant|aAc/aCc|N/T|ENSG00000187583|PLEKHN1|ENST00000379410|1/16|benign(0.444)|deleterious(0.01)|3/611|protein_coding|1 +chr1 902043 . G A 169.05 . AC=2;AF=0.0172;AN=116;BaseQRankSum=-1.369;DP=777;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.0432;InbreedingCoeff=-0.0431;MQ=58.28;MQ0=0;MQRankSum=0.826;QD=5.45;ReadPosRankSum=-0.446;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|1),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|1),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|1),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 902069 . T C 177.69 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.199;DP=717;Dels=0.00;FS=3.490;HRun=4;HaplotypeScore=0.5354;InbreedingCoeff=-0.0340;MQ=58.23;MQ0=0;MQRankSum=0.502;QD=7.11;ReadPosRankSum=0.208;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|1),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|1),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|1),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 902108 . C T 116.44 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.766;DP=558;Dels=0.00;FS=3.848;HRun=0;HaplotypeScore=0.5318;InbreedingCoeff=-0.0325;MQ=58.52;MQ0=0;MQRankSum=-0.417;QD=8.96;ReadPosRankSum=0.186;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A36|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A36|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A36|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,synonymous_variant|gcC/gcT|A|ENSG00000187583|PLEKHN1|ENST00000379409|2/15|||36/663|protein_coding|1,synonymous_variant|gcC/gcT|A|ENSG00000187583|PLEKHN1|ENST00000379407|2/15|||36/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,synonymous_variant|gcC/gcT|A|ENSG00000187583|PLEKHN1|ENST00000379410|2/16|||36/611|protein_coding|1 +chr1 902128 . C T 375.73 . AC=3;AF=0.0254;AN=118;BaseQRankSum=-0.165;DP=545;Dels=0.00;FS=0.993;HRun=1;HaplotypeScore=0.3151;InbreedingCoeff=-0.0508;MQ=58.23;MQ0=0;MQRankSum=0.962;QD=11.39;ReadPosRankSum=-1.116;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A43V|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A43V|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A43V|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,missense_variant|gCt/gTt|A/V|ENSG00000187583|PLEKHN1|ENST00000379409|2/15|possibly_damaging(0.586)|deleterious(0.01)|43/663|protein_coding|1,missense_variant|gCt/gTt|A/V|ENSG00000187583|PLEKHN1|ENST00000379407|2/15|benign(0.209)|deleterious(0.01)|43/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,missense_variant|gCt/gTt|A/V|ENSG00000187583|PLEKHN1|ENST00000379410|2/16|benign(0.209)|deleterious(0.01)|43/611|protein_coding|1 +chr1 902288 . G A 297.6 . AC=8;AF=0.083;AN=96;BaseQRankSum=-1.235;DP=169;Dels=0.00;FS=6.677;HRun=0;HaplotypeScore=0.1735;InbreedingCoeff=0.2484;MQ=58.34;MQ0=0;MQRankSum=1.682;QD=12.94;ReadPosRankSum=-1.820;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|2),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|2),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|2),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 905130 . ATG A 1155.32 . AC=23;AF=0.442;AN=52;BaseQRankSum=1.417;DP=60;FS=3.885;HRun=0;HaplotypeScore=17.3266;InbreedingCoeff=0.3250;MQ=54.35;MQ0=0;MQRankSum=-1.757;QD=30.40;ReadPosRankSum=1.061;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|2),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|2),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|2),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 905158 . TGC T 181.75 . AC=10;AF=0.200;AN=50;BaseQRankSum=-3.797;DP=55;FS=1.587;HRun=0;HaplotypeScore=23.4217;InbreedingCoeff=0.1863;MQ=54.38;MQ0=0;MQRankSum=0.194;QD=12.98;ReadPosRankSum=-2.056;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|2),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|2),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|2),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 905165 . A G 1906.87 . AC=48;AF=0.960;AN=50;BaseQRankSum=0.088;DP=59;Dels=0.00;FS=5.156;HRun=0;HaplotypeScore=0.3866;InbreedingCoeff=0.0421;MQ=56.05;MQ0=0;MQRankSum=1.086;QD=32.88;ReadPosRankSum=-1.380;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|2),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|2),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|2),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 905894 . C T 1520.95 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-2.494;DP=3276;Dels=0.00;FS=2.372;HRun=0;HaplotypeScore=1.4299;InbreedingCoeff=-0.0172;MQ=58.58;MQ0=0;MQRankSum=0.861;QD=13.46;ReadPosRankSum=-0.683;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|3),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|3),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|3),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 +chr1 906272 . A C 2509.21 . AC=19;AF=0.1583;AN=120;BaseQRankSum=3.923;DP=937;Dels=0.00;FS=33.565;HRun=4;HaplotypeScore=0.7062;InbreedingCoeff=0.0234;MQ=58.16;MQ0=0;MQRankSum=0.166;QD=10.72;ReadPosRankSum=0.823;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),EXON(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|1),SYNONYMOUS_CODING(LOW|SILENT|gcA/gcC|A166|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|gcA/gcC|A166|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|gcA/gcC|A206|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,synonymous_variant|gcA/gcC|A|ENSG00000187583|PLEKHN1|ENST00000379409|5/15|||206/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|1/3||||retained_intron|1,synonymous_variant|gcA/gcC|A|ENSG00000187583|PLEKHN1|ENST00000379407|6/15|||166/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|gcA/gcC|A|ENSG00000187583|PLEKHN1|ENST00000379410|6/16|||166/611|protein_coding|1 +chr1 906302 . C T 240.96 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-1.006;DP=838;Dels=0.00;FS=7.521;HRun=0;HaplotypeScore=0.4687;InbreedingCoeff=-0.0191;MQ=58.08;MQ0=0;MQRankSum=-0.315;QD=8.03;ReadPosRankSum=-2.447;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),EXON(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|1),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A176|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A176|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A216|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,synonymous_variant|gcC/gcT|A|ENSG00000187583|PLEKHN1|ENST00000379409|5/15|||216/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|1/3||||retained_intron|1,synonymous_variant|gcC/gcT|A|ENSG00000187583|PLEKHN1|ENST00000379407|6/15|||176/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|gcC/gcT|A|ENSG00000187583|PLEKHN1|ENST00000379410|6/16|||176/611|protein_coding|1 +chr1 906850 . C T 840.9 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.291;DP=1812;Dels=0.00;FS=2.912;HRun=0;HaplotypeScore=1.3291;InbreedingCoeff=-0.0167;MQ=58.39;MQ0=0;MQRankSum=0.375;QD=15.02;ReadPosRankSum=0.230;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),EXON(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|3),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|8),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|8),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|7),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|3/3||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 906872 . C T 490.99 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.837;DP=1326;Dels=0.00;FS=11.727;HRun=3;HaplotypeScore=0.8532;InbreedingCoeff=-0.0185;MQ=58.72;MQ0=0;MQRankSum=0.435;QD=14.88;ReadPosRankSum=0.427;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),EXON(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|3),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|8),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|8),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|7),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|3/3||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 906974 . G A 150.57 . AC=2;AF=0.0185;AN=108;BaseQRankSum=0.388;DP=247;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1707;InbreedingCoeff=-0.0985;MQ=58.20;MQ0=0;MQRankSum=0.405;QD=12.55;ReadPosRankSum=0.062;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|8),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|8),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|7),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 907023 . G A 49.9 . AC=4;AF=0.048;AN=84;BaseQRankSum=0.466;DP=145;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1426;InbreedingCoeff=0.2013;MQ=59.04;MQ0=0;MQRankSum=0.872;QD=16.63;ReadPosRankSum=-0.015;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|8),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|8),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|7),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 907170 . AG A 1280.62 . AC=36;AF=0.3462;AN=104;BaseQRankSum=3.258;DP=132;FS=0.000;HRun=3;HaplotypeScore=8.3225;InbreedingCoeff=0.2957;MQ=57.69;MQ0=0;MQRankSum=0.182;QD=20.99;ReadPosRankSum=0.423;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|8),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|8),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|7),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 907609 . T C 2023.79 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-2.518;DP=2587;Dels=0.00;FS=0.956;HRun=0;HaplotypeScore=2.5448;InbreedingCoeff=-0.0366;MQ=48.44;MQ0=0;MQRankSum=4.469;QD=10.76;ReadPosRankSum=2.738;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|9),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|9),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|8),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 907622 . G C 3088.17 . AC=7;AF=0.0583;AN=120;BaseQRankSum=-1.813;DP=2382;Dels=0.00;FS=1.696;HRun=0;HaplotypeScore=13.8552;InbreedingCoeff=0.2112;MQ=46.67;MQ0=0;MQRankSum=5.413;QD=12.21;ReadPosRankSum=2.166;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|9),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|9),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|8),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 907631 . A C 2295.85 . AC=35;AF=0.2917;AN=120;BaseQRankSum=-20.997;DP=2266;Dels=0.00;FS=665.053;HRun=7;HaplotypeScore=7.6604;InbreedingCoeff=-0.4342;MQ=46.08;MQ0=0;MQRankSum=-14.611;QD=1.46;ReadPosRankSum=13.032;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|9),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|9),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|8),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 907758 . A G 191.93 . AC=13;AF=0.1140;AN=114;BaseQRankSum=-12.237;DP=1481;Dels=0.01;FS=153.003;HRun=5;HaplotypeScore=5.2587;InbreedingCoeff=-0.1585;MQ=51.66;MQ0=0;MQRankSum=-11.759;QD=0.46;ReadPosRankSum=1.329;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAg/gGg|E319G|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAg/gGg|E331G|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAg/gGg|E371G|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,missense_variant|gAg/gGg|E/G|ENSG00000187583|PLEKHN1|ENST00000379409|9/15|benign(0.005)|tolerated(0.28)|371/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,missense_variant|gAg/gGg|E/G|ENSG00000187583|PLEKHN1|ENST00000379407|10/15|benign(0.001)|tolerated(0.21)|331/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,missense_variant|gAg/gGg|E/G|ENSG00000187583|PLEKHN1|ENST00000379410|10/16|benign(0.003)|tolerated(0.26)|319/611|protein_coding|1 +chr1 907774 . G A 268.81 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.645;DP=1416;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.8695;InbreedingCoeff=-0.0177;MQ=51.53;MQ0=0;MQRankSum=0.725;QD=7.47;ReadPosRankSum=-2.464;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),SYNONYMOUS_CODING(LOW|SILENT|ctG/ctA|L324|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|ctG/ctA|L336|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|ctG/ctA|L376|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,synonymous_variant|ctG/ctA|L|ENSG00000187583|PLEKHN1|ENST00000379409|9/15|||376/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,synonymous_variant|ctG/ctA|L|ENSG00000187583|PLEKHN1|ENST00000379407|10/15|||336/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|ctG/ctA|L|ENSG00000187583|PLEKHN1|ENST00000379410|10/16|||324/611|protein_coding|1 +chr1 907816 . A G 331.68 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.634;DP=1030;Dels=0.00;FS=2.656;HRun=0;HaplotypeScore=0.8456;InbreedingCoeff=-0.0346;MQ=55.40;MQ0=0;MQRankSum=0.355;QD=10.05;ReadPosRankSum=3.222;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|10),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|10),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|9),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 907920 . C G 216.19 . AC=4;AF=0.0377;AN=106;BaseQRankSum=0.780;DP=253;Dels=0.00;FS=0.000;HRun=3;HaplotypeScore=0.2690;InbreedingCoeff=0.1203;MQ=58.25;MQ0=0;MQRankSum=-0.123;QD=16.63;ReadPosRankSum=-0.212;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|10),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|10),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|9),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 907991 . CA C 106.79 . AC=5;AF=0.054;AN=92;BaseQRankSum=4.179;DP=101;FS=1.708;HRun=7;HaplotypeScore=8.2099;InbreedingCoeff=0.0500;MQ=56.72;MQ0=0;MQRankSum=-1.342;QD=11.87;ReadPosRankSum=1.095;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|10),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|10),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|9),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 907994 . A C 162.44 . AC=5;AF=0.054;AN=92;BaseQRankSum=3.562;DP=99;Dels=0.00;FS=3.801;HRun=0;HaplotypeScore=0.0865;InbreedingCoeff=0.0684;MQ=56.41;MQ0=0;MQRankSum=-2.224;QD=16.24;ReadPosRankSum=1.331;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|10),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|10),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|9),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 907998 . A T 165.43 . AC=5;AF=0.053;AN=94;BaseQRankSum=2.279;DP=96;Dels=0.00;FS=1.648;HRun=2;HaplotypeScore=0.0847;InbreedingCoeff=0.0537;MQ=56.29;MQ0=0;MQRankSum=-1.944;QD=18.38;ReadPosRankSum=1.172;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|10),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|10),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|9),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 908170 . C G 938.77 . AC=9;AF=0.0763;AN=118;BaseQRankSum=-2.147;DP=536;Dels=0.00;FS=1.531;HRun=2;HaplotypeScore=0.5568;InbreedingCoeff=0.1081;MQ=53.86;MQ0=0;MQRankSum=0.079;QD=11.31;ReadPosRankSum=0.858;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|10),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|10),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|9),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 908228 . A G 406.65 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.194;DP=1035;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5043;InbreedingCoeff=-0.0201;MQ=55.14;MQ0=0;MQRankSum=1.782;QD=12.32;ReadPosRankSum=-0.055;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|10),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|10),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|9),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 908275 . G A 810.31 . AC=3;AF=0.0250;AN=120;BaseQRankSum=0.050;DP=1417;Dels=0.00;FS=4.591;HRun=0;HaplotypeScore=0.6695;InbreedingCoeff=-0.0308;MQ=55.19;MQ0=0;MQRankSum=1.728;QD=10.39;ReadPosRankSum=0.854;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gAc|G346D|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gAc|G358D|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gAc|G398D|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,missense_variant|gGc/gAc|G/D|ENSG00000187583|PLEKHN1|ENST00000379409|10/15|benign(0.006)|tolerated(1)|398/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,missense_variant|gGc/gAc|G/D|ENSG00000187583|PLEKHN1|ENST00000379407|11/15|benign(0.006)|tolerated(1)|358/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,missense_variant|gGc/gAc|G/D|ENSG00000187583|PLEKHN1|ENST00000379410|11/16|benign(0.002)|tolerated(1)|346/611|protein_coding|1 +chr1 908325 . A C 267.79 . AC=14;AF=0.1167;AN=120;BaseQRankSum=-17.215;DP=1827;Dels=0.00;FS=693.401;HRun=2;HaplotypeScore=3.2868;InbreedingCoeff=-0.1506;MQ=55.13;MQ0=0;MQRankSum=-1.642;QD=0.79;ReadPosRankSum=0.894;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Acc/Ccc|T363P|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Acc/Ccc|T375P|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Acc/Ccc|T415P|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,missense_variant|Acc/Ccc|T/P|ENSG00000187583|PLEKHN1|ENST00000379409|10/15|probably_damaging(0.998)|tolerated(0.08)|415/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,missense_variant|Acc/Ccc|T/P|ENSG00000187583|PLEKHN1|ENST00000379407|11/15|probably_damaging(0.998)|tolerated(0.09)|375/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,missense_variant|Acc/Ccc|T/P|ENSG00000187583|PLEKHN1|ENST00000379410|11/16|probably_damaging(0.995)|tolerated(0.11)|363/611|protein_coding|1 +chr1 908414 . C T 3063.53 . AC=7;AF=0.0583;AN=120;BaseQRankSum=-1.682;DP=2413;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.8594;InbreedingCoeff=-0.0636;MQ=57.64;MQ0=0;MQRankSum=2.858;QD=12.66;ReadPosRankSum=0.752;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|11),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|11),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|10),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 908823 . G A 12829.44 . AC=23;AF=0.1917;AN=120;BaseQRankSum=-0.746;DP=2383;Dels=0.00;FS=3.440;HRun=0;HaplotypeScore=1.6658;InbreedingCoeff=-0.0220;MQ=59.01;MQ0=0;MQRankSum=0.792;QD=15.61;ReadPosRankSum=1.037;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|11),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|12),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|11),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 909053 . G A 230.8 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.716;DP=1523;Dels=0.00;FS=1.669;HRun=1;HaplotypeScore=1.0522;InbreedingCoeff=-0.0166;MQ=59.32;MQ0=0;MQRankSum=-0.371;QD=14.43;ReadPosRankSum=0.854;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|1),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|12),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|13),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|12);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 909073 . C T 14861.74 . AC=51;AF=0.4250;AN=120;BaseQRankSum=-2.886;DP=1297;Dels=0.00;FS=1.649;HRun=1;HaplotypeScore=0.9560;InbreedingCoeff=-0.2187;MQ=59.41;MQ0=0;MQRankSum=-0.581;QD=17.08;ReadPosRankSum=-1.066;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|1),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|12),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|13),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|12);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 909221 . T C 1120.5 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-0.121;DP=1389;Dels=0.00;FS=9.505;HRun=0;HaplotypeScore=0.7261;InbreedingCoeff=-0.0453;MQ=58.91;MQ0=0;MQRankSum=0.284;QD=11.55;ReadPosRankSum=3.442;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),SYNONYMOUS_CODING(LOW|SILENT|agT/agC|S446|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|agT/agC|S46|176|PLEKHN1|protein_coding|CODING|ENST00000491024|),SYNONYMOUS_CODING(LOW|SILENT|agT/agC|S481|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|agT/agC|S533|663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,synonymous_variant|agT/agC|S|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|||46/176|protein_coding|1,synonymous_variant|agT/agC|S|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|||533/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,synonymous_variant|agT/agC|S|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|||446/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|agT/agC|S|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|||481/611|protein_coding|1 +chr1 909222 . G A 252.5 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.084;DP=1392;Dels=0.00;FS=1.211;HRun=0;HaplotypeScore=0.7091;InbreedingCoeff=-0.0090;MQ=58.92;MQ0=0;MQRankSum=0.700;QD=9.71;ReadPosRankSum=0.715;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A447T|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A47T|176|PLEKHN1|protein_coding|CODING|ENST00000491024|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A482T|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A534T|663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,missense_variant|Gcc/Acc|A/T|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|benign(0.273)|tolerated(0.13)|47/176|protein_coding|1,missense_variant|Gcc/Acc|A/T|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|benign(0.273)|tolerated(0.47)|534/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,missense_variant|Gcc/Acc|A/T|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|benign(0.273)|tolerated(0.34)|447/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,missense_variant|Gcc/Acc|A/T|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|benign(0.273)|tolerated(0.48)|482/611|protein_coding|1 +chr1 909238 . G C 30583.03 . AC=81;AF=0.6750;AN=120;BaseQRankSum=0.003;DP=1510;Dels=0.00;FS=15.547;HRun=1;HaplotypeScore=2.2700;InbreedingCoeff=-0.0333;MQ=58.99;MQ0=0;MQRankSum=-0.442;QD=22.47;ReadPosRankSum=2.247;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGt/cCt|R452P|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGt/cCt|R487P|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGt/cCt|R52P|176|PLEKHN1|protein_coding|CODING|ENST00000491024|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGt/cCt|R539P|663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,missense_variant|cGt/cCt|R/P|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|benign(0)|tolerated(0.17)|52/176|protein_coding|1,missense_variant|cGt/cCt|R/P|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|benign(0)|tolerated(0.25)|539/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,missense_variant|cGt/cCt|R/P|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|benign(0)|tolerated(0.23)|452/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,missense_variant|cGt/cCt|R/P|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|benign(0)|tolerated(0.23)|487/611|protein_coding|1 +chr1 909242 . A G 1739.34 . AC=6;AF=0.0500;AN=120;BaseQRankSum=1.162;DP=1524;Dels=0.00;FS=7.264;HRun=2;HaplotypeScore=2.2302;InbreedingCoeff=-0.0532;MQ=58.97;MQ0=0;MQRankSum=0.469;QD=11.75;ReadPosRankSum=2.640;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G453|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G488|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G53|176|PLEKHN1|protein_coding|CODING|ENST00000491024|),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G540|663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,synonymous_variant|ggA/ggG|G|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|||53/176|protein_coding|1,synonymous_variant|ggA/ggG|G|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|||540/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,synonymous_variant|ggA/ggG|G|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|||453/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|ggA/ggG|G|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|||488/611|protein_coding|1 +chr1 909309 . T C 7707.57 . AC=26;AF=0.2167;AN=120;BaseQRankSum=5.567;DP=1519;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.7523;InbreedingCoeff=-0.0786;MQ=58.79;MQ0=0;MQRankSum=1.234;QD=13.69;ReadPosRankSum=1.249;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tcc/Ccc|S476P|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tcc/Ccc|S511P|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tcc/Ccc|S563P|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tcc/Ccc|S76P|176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,missense_variant|Tcc/Ccc|S/P|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|benign(0.013)|tolerated(0.25)|76/176|protein_coding|1,missense_variant|Tcc/Ccc|S/P|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|benign(0.013)|tolerated(0.3)|563/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,missense_variant|Tcc/Ccc|S/P|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|benign(0)|tolerated(0.3)|476/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,missense_variant|Tcc/Ccc|S/P|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|benign(0.02)|tolerated(0.29)|511/611|protein_coding|1 +chr1 909320 . C T 232.62 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.426;DP=1387;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.8928;InbreedingCoeff=-0.0090;MQ=58.83;MQ0=0;MQRankSum=0.287;QD=10.57;ReadPosRankSum=2.476;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P479|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P514|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P566|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P79|176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,synonymous_variant|ccC/ccT|P|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|||79/176|protein_coding|1,synonymous_variant|ccC/ccT|P|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|||566/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,synonymous_variant|ccC/ccT|P|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|||479/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|ccC/ccT|P|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|||514/611|protein_coding|1 +chr1 909326 . C T 373.87 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.393;DP=1276;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.8069;InbreedingCoeff=-0.0180;MQ=58.80;MQ0=0;MQRankSum=0.099;QD=8.90;ReadPosRankSum=1.847;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G481|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G516|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G568|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G81|176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,synonymous_variant|ggC/ggT|G|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|||81/176|protein_coding|1,synonymous_variant|ggC/ggT|G|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|||568/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,synonymous_variant|ggC/ggT|G|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|||481/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|ggC/ggT|G|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|||516/611|protein_coding|1 +chr1 909363 . A C 203.29 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.679;DP=1010;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.5429;InbreedingCoeff=-0.0117;MQ=59.04;MQ0=0;MQRankSum=0.409;QD=11.29;ReadPosRankSum=2.880;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),SYNONYMOUS_CODING(LOW|SILENT|Aga/Cga|R494|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|Aga/Cga|R529|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|Aga/Cga|R581|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),SYNONYMOUS_CODING(LOW|SILENT|Aga/Cga|R94|176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,synonymous_variant|Aga/Cga|R|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|||94/176|protein_coding|1,synonymous_variant|Aga/Cga|R|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|||581/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,synonymous_variant|Aga/Cga|R|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|||494/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|Aga/Cga|R|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|||529/611|protein_coding|1 +chr1 909419 . C T 1837.1 . AC=16;AF=0.1333;AN=120;BaseQRankSum=-3.072;DP=694;Dels=0.00;FS=2.424;HRun=0;HaplotypeScore=0.2284;InbreedingCoeff=-0.0370;MQ=58.46;MQ0=0;MQRankSum=0.397;QD=12.17;ReadPosRankSum=1.761;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D112|176|PLEKHN1|protein_coding|CODING|ENST00000491024|),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D512|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D547|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D599|663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,synonymous_variant|gaC/gaT|D|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|||112/176|protein_coding|1,synonymous_variant|gaC/gaT|D|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|||599/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,synonymous_variant|gaC/gaT|D|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|||512/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|gaC/gaT|D|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|||547/611|protein_coding|1 +chr1 909523 . G A 539.82 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.755;DP=1030;Dels=0.00;FS=12.426;HRun=1;HaplotypeScore=0.5627;InbreedingCoeff=-0.0151;MQ=58.16;MQ0=0;MQRankSum=0.665;QD=10.80;ReadPosRankSum=0.177;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|2),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|13),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|14),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|13);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 909554 . CA C 2082.11 . AC=48;AF=0.4000;AN=120;BaseQRankSum=7.755;DP=1646;FS=15.954;HRun=1;HaplotypeScore=179.7893;InbreedingCoeff=-0.1499;MQ=58.00;MQ0=0;MQRankSum=2.728;QD=1.74;ReadPosRankSum=-12.687;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|2),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|13),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|14),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|13);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 909555 . A G 55343.84 . AC=113;AF=0.9417;AN=120;BaseQRankSum=2.487;DP=1646;Dels=0.01;FS=6.244;HRun=6;HaplotypeScore=1.1341;InbreedingCoeff=-0.0625;MQ=58.00;MQ0=0;MQRankSum=0.381;QD=33.62;ReadPosRankSum=-0.430;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|2),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|13),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|14),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|13);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 909768 . A G 183199.18 . AC=120;AF=1.0000;AN=120;BaseQRankSum=0.728;DP=5372;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=2.6976;InbreedingCoeff=-0.0000;MQ=58.76;MQ0=0;MQRankSum=2.388;QD=34.10;ReadPosRankSum=1.847;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|3),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|14),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|15),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|14);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 +chr1 909960 . C T 318.47 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-0.416;DP=1592;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.2673;InbreedingCoeff=-0.0090;MQ=58.66;MQ0=0;MQRankSum=-0.101;QD=11.80;ReadPosRankSum=1.233;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),UTR_3_PRIME(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|4),UTR_3_PRIME(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|15),UTR_3_PRIME(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|16),UTR_3_PRIME(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|15);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,3_prime_UTR_variant|||ENSG00000187583|PLEKHN1|ENST00000491024|4/5|||-/176|protein_coding|1,3_prime_UTR_variant|||ENSG00000187583|PLEKHN1|ENST00000379409|15/15|||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187583|PLEKHN1|ENST00000379407|15/15|||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187583|PLEKHN1|ENST00000379410|16/16|||-/611|protein_coding|1 +chr1 910394 . C T 398.09 . AC=13;AF=0.361;AN=36;BaseQRankSum=1.775;DP=46;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0554;InbreedingCoeff=0.3755;MQ=58.99;MQ0=0;MQRankSum=0.823;QD=23.42;ReadPosRankSum=0.051;EFF=DOWNSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|4),UTR_3_PRIME(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|15),UTR_3_PRIME(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|16);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187583|PLEKHN1|ENST00000379410|16/16|||-/611|protein_coding|1 +chr1 910438 . A G 1374.53 . AC=42;AF=1.000;AN=42;DP=42;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=-0.1458;MQ=57.77;MQ0=0;QD=33.53;EFF=DOWNSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|4),UTR_3_PRIME(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|15),UTR_3_PRIME(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|16);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187583|PLEKHN1|ENST00000379410|16/16|||-/611|protein_coding|1 +chr1 910473 . G A 299.74 . AC=11;AF=0.250;AN=44;BaseQRankSum=-1.316;DP=64;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0000;InbreedingCoeff=0.1771;MQ=59.28;MQ0=0;MQRankSum=0.212;QD=12.49;ReadPosRankSum=-0.144;EFF=DOWNSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|4),UTR_3_PRIME(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|15),UTR_3_PRIME(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|16);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187583|PLEKHN1|ENST00000379410|16/16|||-/611|protein_coding|1 +chr1 934099 . G C 525.65 . AC=24;AF=1.000;AN=24;DP=17;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=-0.1752;MQ=57.23;MQ0=0;QD=30.92;EFF=DOWNSTREAM(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|),DOWNSTREAM(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|),DOWNSTREAM(MODIFIER||||247|HES4|protein_coding|CODING|ENST00000428771|),DOWNSTREAM(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|),INTERGENIC(MODIFIER|||||||||);CSQ=downstream_gene_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000428771||||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000481869|||||retained_intron|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 +chr1 934106 . A G 492.03 . AC=22;AF=1.000;AN=22;DP=17;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=-0.1738;MQ=57.23;MQ0=0;QD=30.75;EFF=DOWNSTREAM(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|),DOWNSTREAM(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|),DOWNSTREAM(MODIFIER||||247|HES4|protein_coding|CODING|ENST00000428771|),DOWNSTREAM(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|),INTERGENIC(MODIFIER|||||||||);CSQ=downstream_gene_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000428771||||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000481869|||||retained_intron|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 +chr1 934130 . G C 64.94 . AC=4;AF=0.222;AN=18;BaseQRankSum=-0.306;DP=15;Dels=0.00;FS=2.515;HRun=0;HaplotypeScore=0.2219;MQ=56.85;MQ0=0;MQRankSum=-0.551;QD=12.99;ReadPosRankSum=2.388;EFF=DOWNSTREAM(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|),DOWNSTREAM(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|),DOWNSTREAM(MODIFIER||||247|HES4|protein_coding|CODING|ENST00000428771|),DOWNSTREAM(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|),INTERGENIC(MODIFIER|||||||||);CSQ=downstream_gene_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000428771||||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000481869|||||retained_intron|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 +chr1 934797 . T G 52.82 . AC=6;AF=0.067;AN=90;BaseQRankSum=-1.481;DP=224;Dels=0.00;FS=43.747;HRun=4;HaplotypeScore=0.0853;InbreedingCoeff=-0.0492;MQ=49.44;MQ0=0;MQRankSum=-1.914;QD=1.51;ReadPosRankSum=0.524;EFF=EXON(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|2),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAc/gCc|D103A|221|HES4|protein_coding|CODING|ENST00000304952|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAc/gCc|D129A|247|HES4|protein_coding|CODING|ENST00000428771|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAc/gCc|D71A|189|HES4|protein_coding|CODING|ENST00000484667|);CSQ=missense_variant|gAc/gCc|D/A|ENSG00000188290|HES4|ENST00000484667|3/3|probably_damaging(0.994)|deleterious(0)|71/189|protein_coding|1,missense_variant|gAc/gCc|D/A|ENSG00000188290|HES4|ENST00000428771|3/3|benign(0.358)|deleterious(0)|129/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188290|HES4|ENST00000481869|2/2||||retained_intron|1,missense_variant|gAc/gCc|D/A|ENSG00000188290|HES4|ENST00000304952|4/4|probably_damaging(0.979)|deleterious(0)|103/221|protein_coding|1 +chr1 934888 . C A 233.29 . AC=2;AF=0.0172;AN=116;BaseQRankSum=-1.550;DP=738;Dels=0.00;FS=2.017;HRun=0;HaplotypeScore=1.1956;InbreedingCoeff=-0.0253;MQ=56.42;MQ0=0;MQRankSum=-0.145;QD=7.07;ReadPosRankSum=-0.918;EFF=INTRON(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|2),INTRON(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|3),INTRON(MODIFIER||||247|HES4|protein_coding|CODING|ENST00000428771|2),INTRON(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|1);CSQ=intron_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,intron_variant|||ENSG00000188290|HES4|ENST00000428771||||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,intron_variant&nc_transcript_variant|||ENSG00000188290|HES4|ENST00000481869|||||retained_intron|1,intron_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 +chr1 934990 . A G 108.0 . AC=12;AF=0.1017;AN=118;BaseQRankSum=-13.261;DP=1923;Dels=0.00;FS=97.152;HRun=3;HaplotypeScore=3.2884;InbreedingCoeff=-0.1051;MQ=46.21;MQ0=0;MQRankSum=-9.305;QD=0.35;ReadPosRankSum=0.938;EFF=EXON(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tcc/Ccc|S38P|189|HES4|protein_coding|CODING|ENST00000484667|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tcc/Ccc|S70P|221|HES4|protein_coding|CODING|ENST00000304952|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tcc/Ccc|S96P|247|HES4|protein_coding|CODING|ENST00000428771|);CSQ=missense_variant|Tcc/Ccc|S/P|ENSG00000188290|HES4|ENST00000484667|2/3|probably_damaging(0.979)|deleterious(0)|38/189|protein_coding|1,missense_variant|Tcc/Ccc|S/P|ENSG00000188290|HES4|ENST00000428771|2/3|benign(0.444)|deleterious(0.02)|96/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188290|HES4|ENST00000481869|1/2||||retained_intron|1,missense_variant|Tcc/Ccc|S/P|ENSG00000188290|HES4|ENST00000304952|3/4|possibly_damaging(0.534)|deleterious(0.01)|70/221|protein_coding|1 +chr1 935046 . G A 2684.85 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-3.713;DP=2876;Dels=0.00;FS=0.372;HRun=0;HaplotypeScore=3.4737;InbreedingCoeff=-0.0181;MQ=51.17;MQ0=0;MQRankSum=0.144;QD=11.88;ReadPosRankSum=0.789;EFF=EXON(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|1),INTRON(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|1),INTRON(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|2),INTRON(MODIFIER||||247|HES4|protein_coding|CODING|ENST00000428771|1);CSQ=intron_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,intron_variant|||ENSG00000188290|HES4|ENST00000428771||||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188290|HES4|ENST00000481869|1/2||||retained_intron|1,intron_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 +chr1 935222 . C A 15396.75 . AC=74;AF=0.6167;AN=120;BaseQRankSum=-9.471;DP=941;Dels=0.00;FS=3.802;HRun=0;HaplotypeScore=1.0918;InbreedingCoeff=-0.0313;MQ=53.20;MQ0=0;MQRankSum=2.504;QD=20.02;ReadPosRankSum=1.296;EFF=EXON(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|1),INTRON(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|1),INTRON(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|agG/agT|R44S|247|HES4|protein_coding|CODING|ENST00000428771|);CSQ=intron_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,missense_variant|agG/agT|R/S|ENSG00000188290|HES4|ENST00000428771|1/3|benign(0)|tolerated(0.95)|44/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188290|HES4|ENST00000481869|1/2||||retained_intron|1,intron_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 +chr1 935459 . A G 44.02 . AC=4;AF=0.67;AN=6;BaseQRankSum=-0.736;DP=4;Dels=0.00;FS=4.771;HRun=2;HaplotypeScore=0.0000;MQ=47.12;MQ0=0;MQRankSum=0.736;QD=22.01;ReadPosRankSum=0.736;EFF=EXON(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|1),UPSTREAM(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|),UTR_5_PRIME(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|1),UTR_5_PRIME(MODIFIER||||247|HES4|protein_coding|CODING|ENST00000428771|1);CSQ=upstream_gene_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,5_prime_UTR_variant|||ENSG00000188290|HES4|ENST00000428771|1/3|||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188290|HES4|ENST00000481869|1/2||||retained_intron|1,5_prime_UTR_variant|||ENSG00000188290|HES4|ENST00000304952|1/4|||-/221|protein_coding|1 +chr1 935492 . G T 38.54 . AC=4;AF=0.50;AN=8;BaseQRankSum=1.026;DP=5;Dels=0.00;FS=0.000;HRun=3;HaplotypeScore=0.2167;MQ=49.96;MQ0=0;MQRankSum=1.026;QD=19.27;ReadPosRankSum=-1.026;EFF=START_GAINED(LOW||||247|HES4|protein_coding|CODING|ENST00000428771|1),UPSTREAM(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|),UPSTREAM(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|),UPSTREAM(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|);CSQ=upstream_gene_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,5_prime_UTR_variant|||ENSG00000188290|HES4|ENST00000428771|1/3|||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,upstream_gene_variant|||ENSG00000188290|HES4|ENST00000481869|||||retained_intron|1,upstream_gene_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 +chr1 935715 . C T 119.53 . AC=6;AF=0.75;AN=8;BaseQRankSum=0.731;DP=6;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;MQ=60.00;MQ0=0;MQRankSum=0.731;QD=29.88;ReadPosRankSum=0.731;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|),UPSTREAM(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|),UPSTREAM(MODIFIER||||247|HES4|protein_coding|CODING|ENST00000428771|),UPSTREAM(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|);CSQ=upstream_gene_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,upstream_gene_variant|||ENSG00000188290|HES4|ENST00000428771||||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,upstream_gene_variant|||ENSG00000188290|HES4|ENST00000481869|||||retained_intron|1,upstream_gene_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 +chr1 935833 . C G 205.67 . AC=10;AF=1.00;AN=10;DP=7;Dels=0.00;FS=0.000;HRun=4;HaplotypeScore=0.0000;MQ=56.62;MQ0=0;QD=29.38;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|),UPSTREAM(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|),UPSTREAM(MODIFIER||||247|HES4|protein_coding|CODING|ENST00000428771|),UPSTREAM(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|);CSQ=upstream_gene_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,upstream_gene_variant|||ENSG00000188290|HES4|ENST00000428771||||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,upstream_gene_variant|||ENSG00000188290|HES4|ENST00000481869|||||retained_intron|1,upstream_gene_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 +chr1 948421 . A AAAC 726.92 . AC=22;AF=1.000;AN=22;DP=20;FS=0.000;HRun=0;HaplotypeScore=4.9337;InbreedingCoeff=-0.1734;MQ=46.54;MQ0=0;QD=45.43;EFF=EXON(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|1),UPSTREAM(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|),UPSTREAM(MODIFIER|||||RP11-54O7.10|processed_pseudogene|NON_CODING|ENST00000423619|);CSQ=upstream_gene_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000231702|RP11-54O7.10|ENST00000423619|||||processed_pseudogene|1,non_coding_exon_variant&nc_transcript_variant&feature_elongation|||ENSG00000224969|RP11-54O7.11|ENST00000458555|1/2||||antisense|1 +chr1 948692 . G A 6273.5 . AC=98;AF=0.8909;AN=110;BaseQRankSum=0.890;DP=195;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1286;InbreedingCoeff=0.1904;MQ=57.72;MQ0=0;MQRankSum=0.244;QD=33.91;ReadPosRankSum=-0.202;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=upstream_gene_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 948844 . C CT 12812.2 . AC=110;AF=0.9167;AN=120;BaseQRankSum=12.848;DP=1613;FS=0.638;HRun=2;HaplotypeScore=267.6122;InbreedingCoeff=0.3293;MQ=57.07;MQ0=0;MQRankSum=-0.300;QD=8.17;ReadPosRankSum=0.729;EFF=UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|),UTR_5_PRIME(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1);CSQ=5_prime_UTR_variant&feature_elongation|||ENSG00000187608|ISG15|ENST00000379389|1/2|||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 948846 . T A 156.11 . AC=8;AF=0.0667;AN=120;BaseQRankSum=-3.754;DP=1643;Dels=0.00;FS=73.746;HRun=1;HaplotypeScore=23.6593;InbreedingCoeff=-0.0805;MQ=57.11;MQ0=0;MQRankSum=1.518;QD=0.75;ReadPosRankSum=-12.345;EFF=UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|),UTR_5_PRIME(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1);CSQ=5_prime_UTR_variant|||ENSG00000187608|ISG15|ENST00000379389|1/2|||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 948846 . T TA 63344.05 . AC=112;AF=0.9333;AN=120;BaseQRankSum=7.480;DP=1716;FS=0.000;HRun=1;HaplotypeScore=206.0209;InbreedingCoeff=-0.0721;MQ=56.50;MQ0=0;MQRankSum=0.146;QD=36.91;ReadPosRankSum=-0.823;EFF=UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|),UTR_5_PRIME(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1);CSQ=5_prime_UTR_variant&feature_elongation|||ENSG00000187608|ISG15|ENST00000379389|1/2|||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 948869 . C T 230.26 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.821;DP=2138;Dels=0.00;FS=1.991;HRun=0;HaplotypeScore=1.4232;InbreedingCoeff=-0.0088;MQ=56.52;MQ0=0;MQRankSum=2.379;QD=11.51;ReadPosRankSum=-0.420;EFF=UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|),UTR_5_PRIME(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1);CSQ=5_prime_UTR_variant|||ENSG00000187608|ISG15|ENST00000379389|1/2|||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 948870 . C G 69163.2 . AC=112;AF=0.9333;AN=120;BaseQRankSum=0.502;DP=2189;Dels=0.00;FS=1.037;HRun=1;HaplotypeScore=1.4157;InbreedingCoeff=-0.0719;MQ=56.60;MQ0=0;MQRankSum=-0.899;QD=31.60;ReadPosRankSum=-1.945;EFF=UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|),UTR_5_PRIME(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1);CSQ=5_prime_UTR_variant|||ENSG00000187608|ISG15|ENST00000379389|1/2|||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 948921 . T C 105377.26 . AC=112;AF=0.9333;AN=120;BaseQRankSum=0.554;DP=3568;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=2.5426;InbreedingCoeff=-0.0712;MQ=57.76;MQ0=0;MQRankSum=-1.329;QD=29.53;ReadPosRankSum=-3.423;EFF=UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|),UTR_5_PRIME(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1);CSQ=5_prime_UTR_variant|||ENSG00000187608|ISG15|ENST00000379389|1/2|||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 949053 . C G 1016.17 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.017;DP=2444;Dels=0.00;FS=9.350;HRun=2;HaplotypeScore=1.7725;InbreedingCoeff=0.6430;MQ=57.83;MQ0=0;MQRankSum=-0.809;QD=17.22;ReadPosRankSum=0.511;EFF=INTRON(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=intron_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 949123 . G A 102.54 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.767;DP=1126;Dels=0.00;FS=2.226;HRun=0;HaplotypeScore=0.7464;InbreedingCoeff=-0.0173;MQ=58.11;MQ0=0;MQRankSum=0.187;QD=6.03;ReadPosRankSum=-1.626;EFF=INTRON(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=intron_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 949235 . G A 32604.9 . AC=112;AF=0.9333;AN=120;BaseQRankSum=-0.988;DP=993;Dels=0.00;FS=7.691;HRun=0;HaplotypeScore=0.4657;InbreedingCoeff=-0.0787;MQ=58.29;MQ0=0;MQRankSum=-1.787;QD=32.83;ReadPosRankSum=-1.094;EFF=INTRON(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=intron_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 949302 . G A 113.2 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.449;DP=1914;Dels=0.00;FS=2.069;HRun=0;HaplotypeScore=1.3393;InbreedingCoeff=-0.0087;MQ=58.25;MQ0=0;MQRankSum=1.862;QD=8.71;ReadPosRankSum=1.655;EFF=INTRON(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=intron_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 949422 . G A 479.41 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.293;DP=3045;Dels=0.00;FS=6.680;HRun=1;HaplotypeScore=1.7335;InbreedingCoeff=-0.0084;MQ=58.77;MQ0=0;MQRankSum=-1.068;QD=12.29;ReadPosRankSum=-1.019;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGc/aAc|S21N|165|ISG15|protein_coding|CODING|ENST00000379389|),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=missense_variant|aGc/aAc|S/N|ENSG00000187608|ISG15|ENST00000379389|2/2|benign(0)|tolerated(0.74)|21/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 949608 . G A 34851.76 . AC=42;AF=0.3500;AN=120;BaseQRankSum=0.005;DP=2776;Dels=0.00;FS=11.466;HRun=1;HaplotypeScore=1.6893;InbreedingCoeff=-0.0991;MQ=58.46;MQ0=0;MQRankSum=1.157;QD=19.90;ReadPosRankSum=1.625;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGc/aAc|S83N|165|ISG15|protein_coding|CODING|ENST00000379389|),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=missense_variant|aGc/aAc|S/N|ENSG00000187608|ISG15|ENST00000379389|2/2|benign(0.002)|tolerated(0.25)|83/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 949654 . A G 103598.95 . AC=108;AF=0.9000;AN=120;BaseQRankSum=3.046;DP=3818;Dels=0.00;FS=9.083;HRun=0;HaplotypeScore=2.1911;InbreedingCoeff=0.0821;MQ=58.12;MQ0=0;MQRankSum=-0.993;QD=27.83;ReadPosRankSum=-2.811;EFF=SYNONYMOUS_CODING(LOW|SILENT|gtA/gtG|V98|165|ISG15|protein_coding|CODING|ENST00000379389|),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=synonymous_variant|gtA/gtG|V|ENSG00000187608|ISG15|ENST00000379389|2/2|||98/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 949655 . C T 133.5 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-2.364;DP=3843;Dels=0.00;FS=4.847;HRun=0;HaplotypeScore=2.2366;InbreedingCoeff=-0.0167;MQ=58.08;MQ0=0;MQRankSum=1.217;QD=2.97;ReadPosRankSum=-1.553;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgg/Tgg|R99W|165|ISG15|protein_coding|CODING|ENST00000379389|),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=missense_variant|Cgg/Tgg|R/W|ENSG00000187608|ISG15|ENST00000379389|2/2|benign(0.007)|tolerated(0.2)|99/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 949831 . C T 3120.79 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.506;DP=2204;Dels=0.00;FS=25.676;HRun=0;HaplotypeScore=2.0744;InbreedingCoeff=-0.0327;MQ=57.33;MQ0=0;MQRankSum=-0.396;QD=12.43;ReadPosRankSum=2.936;EFF=SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G157|165|ISG15|protein_coding|CODING|ENST00000379389|),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=synonymous_variant|ggC/ggT|G|ENSG00000187608|ISG15|ENST00000379389|2/2|||157/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 949832 . G A 1258.11 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.608;DP=2177;Dels=0.00;FS=0.646;HRun=0;HaplotypeScore=2.0191;InbreedingCoeff=-0.0169;MQ=57.31;MQ0=0;MQRankSum=2.168;QD=12.21;ReadPosRankSum=-0.092;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Agc|G158S|165|ISG15|protein_coding|CODING|ENST00000379389|),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=missense_variant|Ggc/Agc|G/S|ENSG00000187608|ISG15|ENST00000379389|2/2|benign(0.009)|tolerated(0.54)|158/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 949884 . A C 542.9 . AC=1;AF=0.0086;AN=116;BaseQRankSum=0.716;DP=1463;Dels=0.00;FS=4.086;HRun=0;HaplotypeScore=1.0630;InbreedingCoeff=-0.0204;MQ=58.08;MQ0=0;MQRankSum=-0.256;QD=14.67;ReadPosRankSum=3.251;EFF=UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|),UTR_3_PRIME(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|2);CSQ=3_prime_UTR_variant|||ENSG00000187608|ISG15|ENST00000379389|2/2|||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 949889 . G A 1254.48 . AC=2;AF=0.0172;AN=116;BaseQRankSum=-1.111;DP=1383;Dels=0.00;FS=3.846;HRun=2;HaplotypeScore=1.0736;InbreedingCoeff=-0.0309;MQ=58.18;MQ0=0;MQRankSum=-0.749;QD=12.93;ReadPosRankSum=2.198;EFF=UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|),UTR_3_PRIME(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|2);CSQ=3_prime_UTR_variant|||ENSG00000187608|ISG15|ENST00000379389|2/2|||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 949925 . C T 25659.52 . AC=103;AF=0.8879;AN=116;BaseQRankSum=0.388;DP=769;Dels=0.00;FS=1.403;HRun=0;HaplotypeScore=0.4309;InbreedingCoeff=0.1833;MQ=58.18;MQ0=0;MQRankSum=0.782;QD=33.54;ReadPosRankSum=-1.626;EFF=DOWNSTREAM(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=downstream_gene_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 950113 . GAAGT G 1437.6 . AC=33;AF=0.532;AN=62;BaseQRankSum=4.162;DP=62;FS=0.000;HRun=0;HaplotypeScore=9.8036;InbreedingCoeff=0.1341;MQ=49.27;MQ0=0;MQRankSum=-5.026;QD=34.23;ReadPosRankSum=-1.205;EFF=DOWNSTREAM(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=downstream_gene_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 950243 . A C 341.55 . AC=21;AF=0.553;AN=38;BaseQRankSum=1.286;DP=25;Dels=0.00;FS=2.282;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=0.2744;MQ=57.17;MQ0=0;MQRankSum=1.615;QD=24.40;ReadPosRankSum=0.099;EFF=DOWNSTREAM(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=downstream_gene_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 +chr1 955016 . A G 41.68 . AC=4;AF=1.00;AN=4;DP=2;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.0000;MQ=47.12;MQ0=0;QD=20.84;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 +chr1 955213 . T C 205.28 . AC=12;AF=1.000;AN=12;DP=7;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.0000;MQ=49.17;MQ0=0;QD=29.33;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 +chr1 955597 . G T 397.16 . AC=19;AF=0.339;AN=56;BaseQRankSum=-2.859;DP=48;Dels=0.00;FS=4.867;HRun=0;HaplotypeScore=0.0297;InbreedingCoeff=0.3749;MQ=53.41;MQ0=0;MQRankSum=0.000;QD=22.06;ReadPosRankSum=2.226;EFF=SYNONYMOUS_CODING(LOW|SILENT|ccG/ccT|P15|2045|AGRN|protein_coding|CODING|ENST00000379370|);CSQ=synonymous_variant|ccG/ccT|P|ENSG00000188157|AGRN|ENST00000379370|1/36|||15/2045|protein_coding|1 +chr1 956061 . A AG 112.73 . AC=10;AF=0.556;AN=18;BaseQRankSum=0.851;DP=13;FS=0.000;HRun=7;HaplotypeScore=6.0534;MQ=48.24;MQ0=0;MQRankSum=-1.268;QD=22.55;ReadPosRankSum=0.103;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|1);CSQ=intron_variant&feature_elongation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 +chr1 956077 . C T 53.71 . AC=5;AF=0.250;AN=20;BaseQRankSum=1.109;DP=15;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=0.1223;MQ=51.77;MQ0=0;MQRankSum=0.370;QD=13.43;ReadPosRankSum=0.925;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 +chr1 957120 . G C 112.86 . AC=7;AF=0.250;AN=28;BaseQRankSum=-0.158;DP=18;Dels=0.00;FS=0.000;HRun=3;HaplotypeScore=0.0000;InbreedingCoeff=0.1430;MQ=57.39;MQ0=0;MQRankSum=-0.791;QD=18.81;ReadPosRankSum=0.791;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 +chr1 957474 . C G 88.48 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.393;DP=913;Dels=0.00;FS=13.654;HRun=0;HaplotypeScore=0.3942;InbreedingCoeff=-0.0138;MQ=58.75;MQ0=0;MQRankSum=0.079;QD=4.02;ReadPosRankSum=2.320;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 +chr1 957568 . A G 559.04 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.476;DP=2810;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.8451;InbreedingCoeff=-0.0085;MQ=58.63;MQ0=0;MQRankSum=0.035;QD=11.41;ReadPosRankSum=2.360;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 +chr1 957640 . C T 832.22 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.040;DP=3915;Dels=0.00;FS=3.301;HRun=0;HaplotypeScore=2.0922;InbreedingCoeff=-0.0143;MQ=58.81;MQ0=0;MQRankSum=0.961;QD=12.06;ReadPosRankSum=1.475;EFF=SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D87|2045|AGRN|protein_coding|CODING|ENST00000379370|);CSQ=synonymous_variant|gaC/gaT|D|ENSG00000188157|AGRN|ENST00000379370|2/36|||87/2045|protein_coding|1 +chr1 957667 . C T 1421.68 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-3.679;DP=4208;Dels=0.00;FS=3.179;HRun=3;HaplotypeScore=2.6513;InbreedingCoeff=-0.0085;MQ=58.87;MQ0=0;MQRankSum=-0.384;QD=13.94;ReadPosRankSum=1.504;EFF=SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G96|2045|AGRN|protein_coding|CODING|ENST00000379370|);CSQ=synonymous_variant|ggC/ggT|G|ENSG00000188157|AGRN|ENST00000379370|2/36|||96/2045|protein_coding|1 +chr1 957898 . G T 11511.79 . AC=35;AF=0.2917;AN=120;BaseQRankSum=-2.213;DP=1017;Dels=0.00;FS=12.996;HRun=0;HaplotypeScore=0.9917;InbreedingCoeff=-0.0796;MQ=55.78;MQ0=0;MQRankSum=2.025;QD=19.92;ReadPosRankSum=0.609;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 +chr1 957967 . T TTGTAGTCTGACCTGTGGTCTGAC 98446.19 . AC=111;AF=0.9737;AN=114;BaseQRankSum=-1.879;DP=448;FS=0.000;HRun=0;HaplotypeScore=117.9611;InbreedingCoeff=-0.0725;MQ=36.18;MQ0=0;MQRankSum=-4.523;QD=220.24;ReadPosRankSum=-0.243;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2);CSQ=intron_variant&feature_elongation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 +chr1 957971 . G A 6538.45 . AC=64;AF=0.5614;AN=114;BaseQRankSum=3.096;DP=423;Dels=0.00;FS=0.554;HRun=0;HaplotypeScore=1.4918;InbreedingCoeff=-0.6704;MQ=34.45;MQ0=0;MQRankSum=-1.012;QD=15.60;ReadPosRankSum=0.359;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 +chr1 957975 . C T 5244.2 . AC=61;AF=0.5446;AN=112;BaseQRankSum=3.205;DP=388;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.4483;InbreedingCoeff=-0.7134;MQ=33.55;MQ0=0;MQRankSum=-2.362;QD=13.66;ReadPosRankSum=-15.499;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 +chr1 957976 . A G 4910.47 . AC=60;AF=0.5455;AN=110;BaseQRankSum=4.309;DP=381;Dels=0.00;FS=1.187;HRun=0;HaplotypeScore=1.4201;InbreedingCoeff=-0.7088;MQ=32.86;MQ0=0;MQRankSum=-2.293;QD=13.03;ReadPosRankSum=-15.805;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 +chr1 958204 . C A 854.8 . AC=2;AF=0.025;AN=80;BaseQRankSum=1.546;DP=704;Dels=0.00;FS=3.962;HRun=1;HaplotypeScore=0.3471;InbreedingCoeff=-0.0900;MQ=57.09;MQ0=0;MQRankSum=0.211;QD=16.13;ReadPosRankSum=1.802;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 +chr1 958236 . T C 367.45 . AC=1;AF=0.013;AN=76;BaseQRankSum=-0.984;DP=1202;Dels=0.00;FS=6.974;HRun=2;HaplotypeScore=0.8541;InbreedingCoeff=-0.0759;MQ=56.81;MQ0=0;MQRankSum=0.871;QD=11.85;ReadPosRankSum=-0.134;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 +chr1 958248 . A G 14600.85 . AC=22;AF=0.297;AN=74;BaseQRankSum=6.262;DP=1397;Dels=0.00;FS=8.143;HRun=2;HaplotypeScore=1.8858;InbreedingCoeff=0.1339;MQ=57.01;MQ0=0;MQRankSum=0.270;QD=19.06;ReadPosRankSum=0.054;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 +chr1 970215 . G C 1422.62 . AC=29;AF=0.483;AN=60;BaseQRankSum=-0.953;DP=94;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0644;InbreedingCoeff=0.4677;MQ=55.42;MQ0=0;MQRankSum=2.956;QD=30.27;ReadPosRankSum=1.616;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 970549 . TGG T 8449.83 . AC=65;AF=0.5603;AN=116;BaseQRankSum=-6.256;DP=1747;FS=120.761;HRun=13;HaplotypeScore=284.3804;InbreedingCoeff=-0.5216;MQ=44.59;MQ0=0;MQRankSum=0.141;QD=5.09;ReadPosRankSum=4.437;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|1);CSQ=intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000469403|1/3||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 970559 . GGGGT G 251.0 . AC=12;AF=0.1017;AN=118;BaseQRankSum=-0.439;DP=2064;FS=2.290;HRun=0;HaplotypeScore=346.6420;InbreedingCoeff=-0.1291;MQ=46.51;MQ0=0;MQRankSum=-12.374;QD=0.60;ReadPosRankSum=6.900;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|1);CSQ=intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000469403|1/3||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 970560 . GGGT G 6238.85 . AC=51;AF=0.4322;AN=118;BaseQRankSum=10.237;DP=2102;FS=59.155;HRun=0;HaplotypeScore=328.0111;InbreedingCoeff=-0.7958;MQ=46.78;MQ0=0;MQRankSum=-6.210;QD=3.10;ReadPosRankSum=17.494;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|1);CSQ=intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000469403|1/3||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 970561 . GGT G 13756.94 . AC=57;AF=0.4831;AN=118;BaseQRankSum=18.075;DP=2129;FS=78.149;HRun=0;HaplotypeScore=306.3538;InbreedingCoeff=-0.8937;MQ=47.00;MQ0=0;MQRankSum=-8.327;QD=6.50;ReadPosRankSum=23.377;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|1);CSQ=intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000469403|1/3||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 970562 . GT G 11726.17 . AC=56;AF=0.4746;AN=118;BaseQRankSum=20.100;DP=2148;FS=44.156;HRun=1;HaplotypeScore=301.2279;InbreedingCoeff=-0.8639;MQ=47.47;MQ0=0;MQRankSum=-8.868;QD=5.54;ReadPosRankSum=21.869;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|1);CSQ=intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000469403|1/3||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 970573 . T G 1645.49 . AC=31;AF=0.2583;AN=120;BaseQRankSum=-22.121;DP=2678;Dels=0.00;FS=172.614;HRun=4;HaplotypeScore=23.1118;InbreedingCoeff=-0.3662;MQ=49.95;MQ0=0;MQRankSum=-11.517;QD=1.06;ReadPosRankSum=19.788;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000469403|1/3||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 970765 . C T 1353.46 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.165;DP=4536;Dels=0.00;FS=22.500;HRun=0;HaplotypeScore=2.7925;InbreedingCoeff=-0.0084;MQ=57.72;MQ0=0;MQRankSum=1.115;QD=12.65;ReadPosRankSum=3.005;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|3),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|2),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 970836 . G A 214.73 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.962;DP=2105;Dels=0.00;FS=7.794;HRun=0;HaplotypeScore=1.1718;InbreedingCoeff=-0.0084;MQ=57.64;MQ0=0;MQRankSum=0.008;QD=10.74;ReadPosRankSum=1.304;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|3),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|2),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 970915 . C T 377.1 . AC=2;AF=0.0167;AN=120;BaseQRankSum=2.244;DP=644;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.3824;InbreedingCoeff=-0.0289;MQ=57.36;MQ0=0;MQRankSum=0.823;QD=9.20;ReadPosRankSum=1.968;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|3),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|2),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 971224 . A G 877.98 . AC=35;AF=0.921;AN=38;BaseQRankSum=0.367;DP=38;Dels=0.00;FS=6.105;HRun=6;HaplotypeScore=0.1297;InbreedingCoeff=0.0071;MQ=58.78;MQ0=0;MQRankSum=0.367;QD=27.44;ReadPosRankSum=0.768;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|3),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|2),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 975702 . A G 736.88 . AC=30;AF=0.833;AN=36;BaseQRankSum=1.380;DP=25;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.0438;InbreedingCoeff=0.2327;MQ=57.17;MQ0=0;MQRankSum=0.794;QD=33.49;ReadPosRankSum=-0.544;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|3),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|2),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 976428 . C A 86.19 . AC=8;AF=1.00;AN=8;DP=10;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5990;MQ=29.00;MQ0=0;QD=21.55;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|4),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|2),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 976514 . C A 153.87 . AC=10;AF=0.833;AN=12;BaseQRankSum=0.742;DP=14;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;MQ=40.36;MQ0=0;MQRankSum=-0.742;QD=25.65;ReadPosRankSum=-0.742;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|4),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|2),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 977203 . G C 14246.35 . AC=102;AF=0.8947;AN=114;BaseQRankSum=0.075;DP=497;Dels=0.00;FS=12.091;HRun=1;HaplotypeScore=0.3092;InbreedingCoeff=0.1183;MQ=53.27;MQ0=0;MQRankSum=-0.225;QD=29.99;ReadPosRankSum=0.784;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|6),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 977220 . C T 77.62 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.664;DP=734;Dels=0.00;FS=6.335;HRun=1;HaplotypeScore=0.5865;InbreedingCoeff=-0.0433;MQ=54.41;MQ0=0;MQRankSum=-0.052;QD=3.53;ReadPosRankSum=0.239;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|6),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 977294 . G A 323.25 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.012;DP=2064;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.9641;InbreedingCoeff=-0.0130;MQ=55.98;MQ0=0;MQRankSum=1.642;QD=9.51;ReadPosRankSum=0.377;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|6),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 977330 . T C 75130.94 . AC=107;AF=0.8917;AN=120;BaseQRankSum=4.114;DP=2805;Dels=0.00;FS=1.138;HRun=2;HaplotypeScore=2.7705;InbreedingCoeff=0.2224;MQ=56.35;MQ0=0;MQRankSum=1.859;QD=27.97;ReadPosRankSum=-0.230;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|6),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=splice_region_variant&intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 977356 . C T 37.67 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.314;DP=3180;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=2.0624;InbreedingCoeff=-0.0085;MQ=56.04;MQ0=0;MQRankSum=1.209;QD=3.42;ReadPosRankSum=0.828;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgg/Tgg|R400W|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=missense_variant|Cgg/Tgg|R/W|ENSG00000188157|AGRN|ENST00000379370|7/36|benign(0.061)|deleterious(0.02)|400/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 977570 . G A 23442.1 . AC=102;AF=0.8500;AN=120;BaseQRankSum=-2.570;DP=882;Dels=0.00;FS=3.065;HRun=0;HaplotypeScore=1.0563;InbreedingCoeff=0.1840;MQ=56.97;MQ0=0;MQRankSum=1.250;QD=27.84;ReadPosRankSum=-0.551;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|7),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 977780 . C T 1896.26 . AC=55;AF=0.833;AN=66;BaseQRankSum=-0.091;DP=70;Dels=0.00;FS=1.840;HRun=0;HaplotypeScore=0.0570;InbreedingCoeff=0.2412;MQ=56.97;MQ0=0;MQRankSum=0.071;QD=30.10;ReadPosRankSum=0.112;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|7),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 978183 . C G 34.17 . AC=2;AF=0.043;AN=46;BaseQRankSum=1.339;DP=53;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0869;InbreedingCoeff=0.1027;MQ=58.24;MQ0=0;MQRankSum=1.491;QD=17.09;ReadPosRankSum=0.025;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|7),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 978193 . G A 202.48 . AC=6;AF=0.125;AN=48;BaseQRankSum=0.284;DP=51;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.0833;InbreedingCoeff=-0.1181;MQ=58.63;MQ0=0;MQRankSum=-0.041;QD=14.46;ReadPosRankSum=0.527;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|7),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 978576 . A AG 514.05 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.254;DP=2624;FS=0.000;HRun=7;HaplotypeScore=255.5270;InbreedingCoeff=-0.0227;MQ=57.44;MQ0=0;MQRankSum=-3.300;QD=9.70;ReadPosRankSum=-2.347;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|7),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 978600 . GCC G 17413.56 . AC=61;AF=0.5169;AN=118;BaseQRankSum=43.323;DP=3497;FS=2.177;HRun=4;HaplotypeScore=348.2579;InbreedingCoeff=-0.1331;MQ=57.63;MQ0=0;MQRankSum=3.380;QD=6.27;ReadPosRankSum=3.269;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|7),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 978603 . CCT C 71936.99 . AC=63;AF=0.5339;AN=118;BaseQRankSum=27.120;DP=3635;FS=0.415;HRun=0;HaplotypeScore=268.7446;InbreedingCoeff=-0.1355;MQ=57.70;MQ0=0;MQRankSum=-0.052;QD=24.97;ReadPosRankSum=2.237;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|7),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 978604 . CTG C 22255.35 . AC=63;AF=0.5339;AN=118;BaseQRankSum=42.643;DP=3702;FS=4.247;HRun=0;HaplotypeScore=358.4425;InbreedingCoeff=-0.1343;MQ=57.72;MQ0=0;MQRankSum=2.535;QD=7.58;ReadPosRankSum=3.410;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|7),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 978762 . G A 3088.0 . AC=4;AF=0.0333;AN=120;BaseQRankSum=0.970;DP=3629;Dels=0.00;FS=0.380;HRun=0;HaplotypeScore=2.3356;InbreedingCoeff=-0.0359;MQ=57.56;MQ0=0;MQRankSum=1.561;QD=14.64;ReadPosRankSum=0.622;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Agc|G510S|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|Ggc/Agc|G/S|ENSG00000188157|AGRN|ENST00000379370|8/36|probably_damaging(0.961)|tolerated(0.05)|510/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 978850 . T G 355.87 . AC=13;AF=0.1083;AN=120;BaseQRankSum=-20.130;DP=2142;Dels=0.00;FS=811.949;HRun=3;HaplotypeScore=4.6408;InbreedingCoeff=-0.1393;MQ=53.60;MQ0=0;MQRankSum=-8.269;QD=0.90;ReadPosRankSum=-1.238;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|8),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 978857 . T G 60.53 . AC=8;AF=0.0667;AN=120;BaseQRankSum=-18.263;DP=2025;Dels=0.01;FS=633.354;HRun=4;HaplotypeScore=4.9061;InbreedingCoeff=-0.0867;MQ=53.43;MQ0=0;MQRankSum=-5.927;QD=0.18;ReadPosRankSum=3.709;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|8),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 979594 . C T 694.16 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.081;DP=5875;DS;Dels=0.00;FS=21.795;HRun=0;HaplotypeScore=3.6311;InbreedingCoeff=-0.0090;MQ=57.97;MQ0=0;MQRankSum=1.076;QD=13.10;ReadPosRankSum=-2.987;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P702L|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|cCg/cTg|P/L|ENSG00000188157|AGRN|ENST00000379370|11/36|probably_damaging(0.931)|tolerated(0.29)|702/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 979669 . G A 1676.8 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-2.241;DP=3939;DS;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=4.9042;InbreedingCoeff=-0.0141;MQ=56.95;MQ0=0;MQRankSum=-0.084;QD=10.10;ReadPosRankSum=1.062;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|11),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 979690 . G A 3048.77 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-3.190;DP=3529;Dels=0.00;FS=4.170;HRun=0;HaplotypeScore=1.8409;InbreedingCoeff=-0.0220;MQ=57.08;MQ0=0;MQRankSum=0.399;QD=11.82;ReadPosRankSum=-0.422;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|11),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 979748 . A T 1423.3 . AC=2;AF=0.0167;AN=120;BaseQRankSum=0.288;DP=3261;Dels=0.00;FS=1.119;HRun=0;HaplotypeScore=1.7332;InbreedingCoeff=-0.0175;MQ=58.27;MQ0=0;MQRankSum=0.306;QD=11.21;ReadPosRankSum=1.431;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAg/gTg|E728V|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|gAg/gTg|E/V|ENSG00000188157|AGRN|ENST00000379370|12/36|probably_damaging(0.932)|deleterious(0.02)|728/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 979835 . G A 1973.32 . AC=2;AF=0.0169;AN=118;BaseQRankSum=-1.744;DP=1701;Dels=0.00;FS=1.140;HRun=0;HaplotypeScore=1.2871;InbreedingCoeff=-0.0241;MQ=58.02;MQ0=0;MQRankSum=1.712;QD=14.00;ReadPosRankSum=0.225;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|12),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 979890 . C T 106.44 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.494;DP=863;Dels=0.00;FS=2.607;HRun=0;HaplotypeScore=0.6567;InbreedingCoeff=-0.0292;MQ=58.16;MQ0=0;MQRankSum=-0.586;QD=9.68;ReadPosRankSum=0.909;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|12),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 980077 . C T 58.05 . AC=1;AF=0.012;AN=86;BaseQRankSum=-0.500;DP=105;Dels=0.00;FS=12.895;HRun=0;HaplotypeScore=0.1629;InbreedingCoeff=-0.1341;MQ=58.00;MQ0=0;MQRankSum=0.378;QD=11.61;ReadPosRankSum=0.765;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|12),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 980189 . G A 62.31 . AC=2;AF=0.032;AN=62;BaseQRankSum=-0.689;DP=65;Dels=0.00;FS=2.622;HRun=0;HaplotypeScore=0.0923;InbreedingCoeff=0.0483;MQ=58.57;MQ0=0;MQRankSum=-0.262;QD=20.77;ReadPosRankSum=-0.131;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|12),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 980276 . C T 58.0 . AC=2;AF=0.021;AN=96;BaseQRankSum=-2.227;DP=125;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1208;InbreedingCoeff=0.0215;MQ=58.51;MQ0=0;MQRankSum=0.682;QD=14.50;ReadPosRankSum=-0.682;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|12),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 980460 . G A 26594.59 . AC=107;AF=0.8917;AN=120;BaseQRankSum=-2.656;DP=975;Dels=0.00;FS=0.883;HRun=0;HaplotypeScore=0.8473;InbreedingCoeff=0.2169;MQ=58.13;MQ0=0;MQRankSum=-0.492;QD=28.60;ReadPosRankSum=0.989;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|12),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 980824 . G C 275.83 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.440;DP=1969;Dels=0.00;FS=12.667;HRun=0;HaplotypeScore=2.5993;InbreedingCoeff=-0.0094;MQ=58.48;MQ0=0;MQRankSum=1.465;QD=11.99;ReadPosRankSum=-0.443;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|1),SYNONYMOUS_CODING(LOW|SILENT|ggG/ggC|G819|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|ggG/ggC|G|ENSG00000188157|AGRN|ENST00000379370|14/36|||819/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000479707|1/4||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 980941 . C T 600.94 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.751;DP=2190;Dels=0.00;FS=4.066;HRun=0;HaplotypeScore=1.5643;InbreedingCoeff=-0.0087;MQ=58.24;MQ0=0;MQRankSum=0.449;QD=12.02;ReadPosRankSum=0.589;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|14),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 980948 . C T 538.98 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.096;DP=2144;Dels=0.00;FS=17.669;HRun=0;HaplotypeScore=2.3599;InbreedingCoeff=-0.0087;MQ=58.24;MQ0=0;MQRankSum=-0.314;QD=17.39;ReadPosRankSum=0.302;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|14),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 980955 . AC A 1099.14 . AC=2;AF=0.0167;AN=120;BaseQRankSum=8.294;DP=2110;FS=28.545;HRun=5;HaplotypeScore=116.2371;InbreedingCoeff=-0.0170;MQ=58.34;MQ0=0;MQRankSum=-0.585;QD=9.16;ReadPosRankSum=1.829;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|14),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 981087 . A G 111674.9 . AC=105;AF=0.8750;AN=120;BaseQRankSum=2.112;DP=3879;Dels=0.00;FS=2.321;HRun=0;HaplotypeScore=2.2416;InbreedingCoeff=0.1619;MQ=58.40;MQ0=0;MQRankSum=-0.442;QD=29.96;ReadPosRankSum=-1.459;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|14),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 +chr1 981131 . A G 3064.9 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-1.865;DP=5182;Dels=0.00;FS=11.038;HRun=0;HaplotypeScore=2.4921;InbreedingCoeff=-0.0256;MQ=58.34;MQ0=0;MQRankSum=1.546;QD=10.79;ReadPosRankSum=1.415;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|2),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cAa/cGa|Q852R|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|cAa/cGa|Q/R|ENSG00000188157|AGRN|ENST00000379370|15/36|benign(0.227)|tolerated(0.44)|852/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000479707|2/4||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 981931 . A G 38481.37 . AC=102;AF=0.8500;AN=120;BaseQRankSum=4.244;DP=1481;Dels=0.00;FS=5.461;HRun=0;HaplotypeScore=0.5832;InbreedingCoeff=0.0829;MQ=57.68;MQ0=0;MQRankSum=2.481;QD=26.85;ReadPosRankSum=-2.762;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),SYNONYMOUS_CODING(LOW|SILENT|tcA/tcG|S1022|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|tcA/tcG|S|ENSG00000188157|AGRN|ENST00000379370|18/36|||1022/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 982205 . G A 372.27 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.314;DP=1529;Dels=0.00;FS=1.129;HRun=1;HaplotypeScore=1.1177;InbreedingCoeff=-0.0134;MQ=58.00;MQ0=0;MQRankSum=0.488;QD=14.32;ReadPosRankSum=-0.923;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gag/Aag|E1086K|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|Gag/Aag|E/K|ENSG00000188157|AGRN|ENST00000379370|19/36|benign(0.32)|tolerated(0.41)|1086/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 982403 . C T 1164.33 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-2.215;DP=1442;Dels=0.00;FS=2.421;HRun=1;HaplotypeScore=0.9843;InbreedingCoeff=-0.0334;MQ=57.51;MQ0=0;MQRankSum=0.119;QD=10.49;ReadPosRankSum=-1.415;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|19),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 982444 . A G 24810.69 . AC=102;AF=0.8500;AN=120;BaseQRankSum=4.056;DP=908;Dels=0.00;FS=0.946;HRun=3;HaplotypeScore=0.5521;InbreedingCoeff=0.0418;MQ=57.33;MQ0=0;MQRankSum=0.383;QD=27.94;ReadPosRankSum=1.506;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|19),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 982462 . T C 27335.24 . AC=119;AF=0.9917;AN=120;BaseQRankSum=0.923;DP=796;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5400;InbreedingCoeff=-0.0264;MQ=57.04;MQ0=0;MQRankSum=0.157;QD=34.34;ReadPosRankSum=-1.750;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|19),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 982482 . C T 1093.25 . AC=3;AF=0.0254;AN=118;BaseQRankSum=0.161;DP=714;Dels=0.00;FS=1.368;HRun=0;HaplotypeScore=0.5694;InbreedingCoeff=-0.0414;MQ=57.13;MQ0=0;MQRankSum=0.077;QD=14.20;ReadPosRankSum=3.205;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|19),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 982484 . C T 1096.42 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.607;DP=707;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5118;InbreedingCoeff=-0.0484;MQ=57.24;MQ0=0;MQRankSum=0.555;QD=14.43;ReadPosRankSum=3.144;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|19),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 982513 . T C 22111.8 . AC=107;AF=0.8917;AN=120;BaseQRankSum=1.897;DP=701;Dels=0.00;FS=6.392;HRun=0;HaplotypeScore=0.5397;InbreedingCoeff=0.3020;MQ=57.59;MQ0=0;MQRankSum=-0.108;QD=32.37;ReadPosRankSum=0.738;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|19),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 982573 . C T 1076.33 . AC=3;AF=0.0250;AN=120;BaseQRankSum=0.536;DP=905;Dels=0.00;FS=13.480;HRun=0;HaplotypeScore=0.6754;InbreedingCoeff=-0.0368;MQ=58.37;MQ0=0;MQRankSum=-0.537;QD=12.23;ReadPosRankSum=1.369;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|19),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 982844 . G C 4135.57 . AC=11;AF=0.0917;AN=120;BaseQRankSum=-1.942;DP=1958;Dels=0.00;FS=2.777;HRun=0;HaplotypeScore=1.2617;InbreedingCoeff=-0.1015;MQ=58.19;MQ0=0;MQRankSum=1.346;QD=12.09;ReadPosRankSum=2.109;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|20),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 982865 . A G 36.62 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.110;DP=1923;Dels=0.00;FS=2.301;HRun=0;HaplotypeScore=1.0780;InbreedingCoeff=-0.0085;MQ=58.17;MQ0=0;MQRankSum=-1.323;QD=5.23;ReadPosRankSum=1.132;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|20),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000478677|1/2||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 982906 . G A 170.75 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.574;DP=1942;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.9590;InbreedingCoeff=-0.0085;MQ=58.80;MQ0=0;MQRankSum=0.287;QD=11.38;ReadPosRankSum=-1.149;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|20),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000478677|1/2||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 982941 . T C 66725.31 . AC=101;AF=0.8417;AN=120;BaseQRankSum=6.926;DP=2345;Dels=0.00;FS=18.520;HRun=3;HaplotypeScore=1.4495;InbreedingCoeff=0.1870;MQ=58.87;MQ0=0;MQRankSum=0.922;QD=29.91;ReadPosRankSum=-2.729;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|20),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000478677|1/2||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 982994 . T C 93842.13 . AC=104;AF=0.8667;AN=120;BaseQRankSum=1.394;DP=3268;Dels=0.00;FS=0.376;HRun=1;HaplotypeScore=1.8496;InbreedingCoeff=0.1346;MQ=58.74;MQ0=0;MQRankSum=1.734;QD=30.11;ReadPosRankSum=-2.989;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|2),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|1),SYNONYMOUS_CODING(LOW|SILENT|ttT/ttC|F1186|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000466223|2/3||||retained_intron|1,synonymous_variant|ttT/ttC|F|ENSG00000188157|AGRN|ENST00000379370|21/36|||1186/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000478677|1/2||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 983005 . G A 980.3 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.923;DP=3454;Dels=0.00;FS=1.269;HRun=0;HaplotypeScore=1.9623;InbreedingCoeff=-0.0084;MQ=58.65;MQ0=0;MQRankSum=1.298;QD=13.62;ReadPosRankSum=2.603;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|2),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGc/cAc|R1190H|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000466223|2/3||||retained_intron|1,missense_variant|cGc/cAc|R/H|ENSG00000188157|AGRN|ENST00000379370|21/36|benign(0.252)|tolerated(0.19)|1190/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000478677|1/2||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 983291 . C G 40.75 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-3.182;DP=508;Dels=0.00;FS=19.277;HRun=1;HaplotypeScore=0.5577;InbreedingCoeff=-0.0586;MQ=54.43;MQ0=0;MQRankSum=-1.088;QD=0.85;ReadPosRankSum=-2.005;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|22),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 984147 . CAAAAAAAAAAA C 4515.7 . AC=46;AF=0.460;AN=100;BaseQRankSum=7.017;DP=179;FS=0.000;HRun=22;HaplotypeScore=47.7401;InbreedingCoeff=0.1656;MQ=37.04;MQ0=0;MQRankSum=-0.993;QD=36.71;ReadPosRankSum=4.302;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|23),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000492947|1/2||||retained_intron|1 +chr1 984170 . GCA G 2088.76 . AC=73;AF=0.6404;AN=114;BaseQRankSum=8.221;DP=284;FS=0.000;HRun=0;HaplotypeScore=52.8663;InbreedingCoeff=0.0057;MQ=43.30;MQ0=0;MQRankSum=-4.981;QD=8.29;ReadPosRankSum=6.043;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|23),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000492947|1/2||||retained_intron|1 +chr1 984171 . CAG C 6456.64 . AC=72;AF=0.6207;AN=116;BaseQRankSum=5.599;DP=294;FS=0.921;HRun=0;HaplotypeScore=45.6091;InbreedingCoeff=-0.0184;MQ=43.76;MQ0=0;MQRankSum=-4.138;QD=26.03;ReadPosRankSum=5.090;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|23),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000492947|1/2||||retained_intron|1 +chr1 984202 . A G 219.31 . AC=2;AF=0.0169;AN=118;BaseQRankSum=-2.549;DP=472;Dels=0.00;FS=8.756;HRun=0;HaplotypeScore=1.0667;InbreedingCoeff=-0.0548;MQ=49.93;MQ0=0;MQRankSum=1.399;QD=7.83;ReadPosRankSum=0.241;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|23),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|1/2||||retained_intron|1 +chr1 984257 . C T 182.2 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.337;DP=770;Dels=0.00;FS=9.908;HRun=0;HaplotypeScore=0.4996;InbreedingCoeff=-0.0252;MQ=54.84;MQ0=0;MQRankSum=1.233;QD=12.15;ReadPosRankSum=-0.369;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A1372|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|gcC/gcT|A|ENSG00000188157|AGRN|ENST00000379370|24/36|||1372/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|1/2||||retained_intron|1 +chr1 984272 . C T 98.86 . AC=2;AF=0.0169;AN=118;BaseQRankSum=-0.517;DP=781;Dels=0.00;FS=1.477;HRun=2;HaplotypeScore=0.5249;InbreedingCoeff=-0.0341;MQ=54.72;MQ0=0;MQRankSum=0.805;QD=3.95;ReadPosRankSum=0.353;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F1377|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|ttC/ttT|F|ENSG00000188157|AGRN|ENST00000379370|24/36|||1377/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|1/2||||retained_intron|1 +chr1 984302 . T C 14311.5 . AC=73;AF=0.6186;AN=118;BaseQRankSum=1.074;DP=888;Dels=0.00;FS=38.864;HRun=1;HaplotypeScore=0.6522;InbreedingCoeff=-0.0933;MQ=54.70;MQ0=0;MQRankSum=1.221;QD=18.54;ReadPosRankSum=0.025;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),SYNONYMOUS_CODING(LOW|SILENT|acT/acC|T1387|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|acT/acC|T|ENSG00000188157|AGRN|ENST00000379370|24/36|||1387/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|1/2||||retained_intron|1 +chr1 984456 . C T 37.75 . AC=2;AF=0.0175;AN=114;BaseQRankSum=0.621;DP=348;Dels=0.01;FS=0.000;HRun=0;HaplotypeScore=0.7641;InbreedingCoeff=-0.0607;MQ=53.36;MQ0=0;MQRankSum=0.580;QD=2.90;ReadPosRankSum=-0.096;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 984476 . T G 35.43 . AC=6;AF=0.060;AN=100;BaseQRankSum=-3.204;DP=227;Dels=0.01;FS=3.373;HRun=3;HaplotypeScore=0.9086;InbreedingCoeff=-0.1488;MQ=48.96;MQ0=0;MQRankSum=-3.544;QD=1.07;ReadPosRankSum=1.813;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 984480 . C G 43.98 . AC=7;AF=0.071;AN=98;BaseQRankSum=-3.119;DP=215;Dels=0.00;FS=2.297;HRun=4;HaplotypeScore=2.0881;InbreedingCoeff=-0.1728;MQ=47.60;MQ0=0;MQRankSum=-4.127;QD=1.47;ReadPosRankSum=3.119;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 984485 . A G 61.07 . AC=8;AF=0.083;AN=96;BaseQRankSum=-3.712;DP=196;Dels=0.02;FS=2.293;HRun=4;HaplotypeScore=2.7170;InbreedingCoeff=-0.0981;MQ=45.76;MQ0=0;MQRankSum=-5.008;QD=2.35;ReadPosRankSum=3.158;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 984489 . G T 64.93 . AC=2;AF=0.021;AN=96;BaseQRankSum=1.248;DP=184;Dels=0.00;FS=2.430;HRun=0;HaplotypeScore=2.0202;InbreedingCoeff=0.0358;MQ=44.34;MQ0=0;MQRankSum=0.975;QD=21.64;ReadPosRankSum=-0.342;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 984489 . GAC G 147.88 . AC=19;AF=0.1863;AN=102;BaseQRankSum=5.670;DP=170;FS=16.798;HRun=0;HaplotypeScore=44.7087;InbreedingCoeff=-0.0321;MQ=43.36;MQ0=0;MQRankSum=-5.590;QD=2.39;ReadPosRankSum=-1.803;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 984494 . GC G 1466.1 . AC=57;AF=0.663;AN=86;BaseQRankSum=2.571;DP=152;FS=2.990;HRun=1;HaplotypeScore=47.8858;InbreedingCoeff=0.1367;MQ=40.49;MQ0=0;MQRankSum=-2.751;QD=11.64;ReadPosRankSum=0.066;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 984501 . A G 452.7 . AC=26;AF=0.317;AN=82;BaseQRankSum=0.750;DP=155;Dels=0.04;FS=57.535;HRun=7;HaplotypeScore=2.7737;InbreedingCoeff=-0.1078;MQ=40.74;MQ0=0;MQRankSum=-5.758;QD=5.73;ReadPosRankSum=3.176;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 984510 . C CG 61.68 . AC=13;AF=0.144;AN=90;BaseQRankSum=4.890;DP=164;FS=70.003;HRun=4;HaplotypeScore=50.6057;InbreedingCoeff=-0.0152;MQ=42.03;MQ0=0;MQRankSum=-4.938;QD=1.50;ReadPosRankSum=-1.662;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 984524 . T G 47.35 . AC=11;AF=0.125;AN=88;BaseQRankSum=-2.019;DP=191;Dels=0.01;FS=48.423;HRun=3;HaplotypeScore=3.1841;InbreedingCoeff=-0.0001;MQ=47.05;MQ0=0;MQRankSum=-3.798;QD=1.01;ReadPosRankSum=3.652;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 984528 . T G 133.69 . AC=15;AF=0.167;AN=90;BaseQRankSum=-2.316;DP=208;Dels=0.01;FS=63.386;HRun=4;HaplotypeScore=2.2156;InbreedingCoeff=-0.0134;MQ=47.68;MQ0=0;MQRankSum=-5.136;QD=2.09;ReadPosRankSum=3.074;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 984533 . T G 92.45 . AC=10;AF=0.111;AN=90;BaseQRankSum=-2.508;DP=221;Dels=0.00;FS=75.206;HRun=4;HaplotypeScore=0.9453;InbreedingCoeff=0.0314;MQ=49.13;MQ0=0;MQRankSum=-5.147;QD=1.71;ReadPosRankSum=0.969;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 984711 . G A 778.12 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.444;DP=2749;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.7477;InbreedingCoeff=-0.0101;MQ=58.27;MQ0=0;MQRankSum=-0.471;QD=11.61;ReadPosRankSum=-0.316;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|2),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGg/cAg|R1465Q|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|cGg/cAg|R/Q|ENSG00000188157|AGRN|ENST00000379370|25/36|benign(0.094)|tolerated(0.53)|1465/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|2/2||||retained_intron|1 +chr1 984847 . G C 1888.19 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.596;DP=3896;Dels=0.00;FS=5.841;HRun=0;HaplotypeScore=3.8645;InbreedingCoeff=-0.0190;MQ=58.46;MQ0=0;MQRankSum=0.505;QD=13.58;ReadPosRankSum=-1.173;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|25),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 984888 . A G 1593.23 . AC=2;AF=0.0167;AN=120;BaseQRankSum=0.251;DP=2785;Dels=0.00;FS=2.468;HRun=0;HaplotypeScore=2.4818;InbreedingCoeff=-0.0179;MQ=58.39;MQ0=0;MQRankSum=2.076;QD=14.75;ReadPosRankSum=3.534;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|25),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 984957 . G A 998.45 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.493;DP=2152;Dels=0.00;FS=5.524;HRun=0;HaplotypeScore=1.2695;InbreedingCoeff=-0.0190;MQ=58.57;MQ0=0;MQRankSum=-0.059;QD=17.83;ReadPosRankSum=1.102;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGg/cAg|R1509Q|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|cGg/cAg|R/Q|ENSG00000188157|AGRN|ENST00000379370|26/36|possibly_damaging(0.459)|tolerated(0.3)|1509/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985165 . C T 53.15 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.519;DP=760;Dels=0.00;FS=6.936;HRun=0;HaplotypeScore=0.5648;InbreedingCoeff=-0.0414;MQ=58.09;MQ0=0;MQRankSum=0.442;QD=3.32;ReadPosRankSum=1.429;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P1578|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|ccC/ccT|P|ENSG00000188157|AGRN|ENST00000379370|26/36|||1578/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985239 . C T 170.83 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.190;DP=977;Dels=0.00;FS=9.028;HRun=0;HaplotypeScore=0.4363;InbreedingCoeff=-0.0324;MQ=58.40;MQ0=0;MQRankSum=-0.599;QD=6.57;ReadPosRankSum=0.946;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|26),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985266 . C T 19790.42 . AC=69;AF=0.5750;AN=120;BaseQRankSum=-0.605;DP=1126;Dels=0.00;FS=27.225;HRun=0;HaplotypeScore=0.6765;InbreedingCoeff=-0.0986;MQ=58.34;MQ0=0;MQRankSum=-0.994;QD=19.93;ReadPosRankSum=-1.430;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|26),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985378 . G A 349.18 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.347;DP=1151;Dels=0.00;FS=36.082;HRun=1;HaplotypeScore=0.7396;InbreedingCoeff=-0.0111;MQ=57.13;MQ0=0;MQRankSum=0.215;QD=14.55;ReadPosRankSum=1.577;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gag/Aag|E1614K|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|Gag/Aag|E/K|ENSG00000188157|AGRN|ENST00000379370|27/36|benign(0.087)|tolerated(0.36)|1614/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985443 . AG A 5524.19 . AC=63;AF=0.5250;AN=120;BaseQRankSum=-4.267;DP=1211;FS=1.118;HRun=15;HaplotypeScore=183.6608;InbreedingCoeff=0.2307;MQ=52.69;MQ0=0;MQRankSum=2.703;QD=6.02;ReadPosRankSum=0.541;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985445 . G GT 4225.58 . AC=52;AF=0.4333;AN=120;BaseQRankSum=0.352;DP=1238;FS=80.878;HRun=0;HaplotypeScore=191.8295;InbreedingCoeff=-0.1480;MQ=52.62;MQ0=0;MQRankSum=2.617;QD=4.70;ReadPosRankSum=-5.379;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985446 . G T 10415.52 . AC=55;AF=0.4583;AN=120;BaseQRankSum=-15.323;DP=1238;Dels=0.00;FS=78.688;HRun=0;HaplotypeScore=12.5413;InbreedingCoeff=-0.0418;MQ=52.62;MQ0=0;MQRankSum=3.979;QD=11.59;ReadPosRankSum=-1.572;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985447 . G T 432.38 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-5.349;DP=1263;Dels=0.00;FS=3.447;HRun=0;HaplotypeScore=12.2057;InbreedingCoeff=-0.0488;MQ=52.38;MQ0=0;MQRankSum=-2.193;QD=3.90;ReadPosRankSum=0.844;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985449 . G A 14507.18 . AC=74;AF=0.6167;AN=120;BaseQRankSum=-12.718;DP=1283;Dels=0.00;FS=44.752;HRun=0;HaplotypeScore=13.5390;InbreedingCoeff=-0.3559;MQ=52.41;MQ0=0;MQRankSum=2.768;QD=12.78;ReadPosRankSum=-2.687;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985450 . G A 9694.98 . AC=38;AF=0.3167;AN=120;BaseQRankSum=-9.217;DP=1307;Dels=0.00;FS=25.729;HRun=0;HaplotypeScore=14.9791;InbreedingCoeff=-0.3897;MQ=52.45;MQ0=0;MQRankSum=4.412;QD=10.72;ReadPosRankSum=2.909;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985458 . GT G 311.7 . AC=16;AF=0.1333;AN=120;BaseQRankSum=4.206;DP=1448;FS=204.972;HRun=2;HaplotypeScore=201.5164;InbreedingCoeff=-0.1309;MQ=53.65;MQ0=0;MQRankSum=1.914;QD=0.64;ReadPosRankSum=10.293;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985460 . T C 3061.71 . AC=16;AF=0.1333;AN=120;BaseQRankSum=-0.490;DP=1456;Dels=0.00;FS=6.540;HRun=0;HaplotypeScore=4.9126;InbreedingCoeff=0.2588;MQ=53.76;MQ0=0;MQRankSum=-0.066;QD=11.78;ReadPosRankSum=0.826;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985509 . G A 2780.67 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.579;DP=2940;Dels=0.00;FS=10.079;HRun=0;HaplotypeScore=1.7811;InbreedingCoeff=-0.0096;MQ=56.77;MQ0=0;MQRankSum=2.214;QD=17.60;ReadPosRankSum=0.249;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985662 . C T 105.9 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-11.359;DP=3310;Dels=0.00;FS=122.177;HRun=2;HaplotypeScore=2.7454;InbreedingCoeff=-0.0439;MQ=57.76;MQ0=0;MQRankSum=-6.298;QD=0.47;ReadPosRankSum=-5.444;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F1643|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|ttC/ttT|F|ENSG00000188157|AGRN|ENST00000379370|28/36|||1643/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985797 . A G 944.49 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.428;DP=2302;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.3924;InbreedingCoeff=-0.0171;MQ=57.79;MQ0=0;MQRankSum=2.462;QD=13.12;ReadPosRankSum=0.537;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|28),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985826 . G A 438.23 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.507;DP=2255;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.1410;InbreedingCoeff=-0.0085;MQ=57.85;MQ0=0;MQRankSum=0.635;QD=14.61;ReadPosRankSum=-0.381;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtc/Atc|V1666I|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|Gtc/Atc|V/I|ENSG00000188157|AGRN|ENST00000379370|29/36|benign(0.119)|tolerated(0.44)|1666/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985855 . C T 288.16 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.233;DP=2094;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.0217;InbreedingCoeff=-0.0087;MQ=58.10;MQ0=0;MQRankSum=0.034;QD=11.53;ReadPosRankSum=-1.888;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G1675|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|ggC/ggT|G|ENSG00000188157|AGRN|ENST00000379370|29/36|||1675/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985900 . C T 558.67 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.271;DP=2177;Dels=0.00;FS=1.593;HRun=2;HaplotypeScore=1.0978;InbreedingCoeff=-0.0209;MQ=58.11;MQ0=0;MQRankSum=0.567;QD=10.16;ReadPosRankSum=-1.144;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F1690|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|ttC/ttT|F|ENSG00000188157|AGRN|ENST00000379370|29/36|||1690/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 985999 . G T 316.5 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.550;DP=1428;Dels=0.00;FS=4.252;HRun=0;HaplotypeScore=0.7980;InbreedingCoeff=-0.0179;MQ=58.27;MQ0=0;MQRankSum=0.065;QD=8.79;ReadPosRankSum=0.661;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|29),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 986038 . G A 80.39 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.606;DP=1099;Dels=0.00;FS=1.750;HRun=0;HaplotypeScore=0.5655;InbreedingCoeff=-0.0135;MQ=57.65;MQ0=0;MQRankSum=0.062;QD=8.04;ReadPosRankSum=0.009;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|29),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 986285 . C T 224.77 . AC=2;AF=0.0169;AN=118;BaseQRankSum=0.797;DP=1956;Dels=0.00;FS=30.697;HRun=0;HaplotypeScore=1.3276;InbreedingCoeff=-0.0312;MQ=54.91;MQ0=0;MQRankSum=2.696;QD=12.49;ReadPosRankSum=-0.681;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|30),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 986286 . C T 649.39 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-0.566;DP=1981;Dels=0.00;FS=22.049;HRun=0;HaplotypeScore=1.6726;InbreedingCoeff=-0.0217;MQ=54.94;MQ0=0;MQRankSum=4.757;QD=11.39;ReadPosRankSum=1.099;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|30),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 986443 . C T 41215.18 . AC=100;AF=0.8621;AN=116;BaseQRankSum=0.781;DP=1363;Dels=0.00;FS=2.212;HRun=0;HaplotypeScore=0.8018;InbreedingCoeff=0.1240;MQ=57.18;MQ0=0;MQRankSum=-1.053;QD=31.27;ReadPosRankSum=-1.007;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|30),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 986737 . T C 414.03 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-2.032;DP=1137;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7186;InbreedingCoeff=-0.0327;MQ=58.21;MQ0=0;MQRankSum=1.287;QD=8.63;ReadPosRankSum=2.285;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),SYNONYMOUS_CODING(LOW|SILENT|ggT/ggC|G1786|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|ggT/ggC|G|ENSG00000188157|AGRN|ENST00000379370|31/36|||1786/2045|protein_coding|1,synonymous_variant|ggT/ggC|G|ENSG00000188157|AGRN|ENST00000419249|4/9|||89/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 986885 . T G 155.26 . AC=7;AF=0.0593;AN=118;BaseQRankSum=-10.562;DP=745;Dels=0.00;FS=53.331;HRun=2;HaplotypeScore=0.5798;InbreedingCoeff=-0.0971;MQ=57.41;MQ0=0;MQRankSum=1.830;QD=1.14;ReadPosRankSum=-2.492;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTg/gGg|V1808G|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|gTg/gGg|V/G|ENSG00000188157|AGRN|ENST00000379370|32/36|possibly_damaging(0.727)|tolerated(0.14)|1808/2045|protein_coding|1,missense_variant|gTg/gGg|V/G|ENSG00000188157|AGRN|ENST00000419249|5/9|possibly_damaging(0.723)|tolerated(0.1)|111/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 986920 . C A 169.9 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.934;DP=839;Dels=0.00;FS=1.422;HRun=0;HaplotypeScore=0.7669;InbreedingCoeff=-0.0288;MQ=58.26;MQ0=0;MQRankSum=0.709;QD=10.62;ReadPosRankSum=2.238;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),SYNONYMOUS_CODING(LOW|SILENT|Cgg/Agg|R1820|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|Cgg/Agg|R|ENSG00000188157|AGRN|ENST00000379370|32/36|||1820/2045|protein_coding|1,synonymous_variant|Cgg/Agg|R|ENSG00000188157|AGRN|ENST00000419249|5/9|||123/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 987200 . C T 92501.43 . AC=100;AF=0.8333;AN=120;BaseQRankSum=1.193;DP=3392;Dels=0.00;FS=27.772;HRun=0;HaplotypeScore=2.0645;InbreedingCoeff=0.1600;MQ=56.45;MQ0=0;MQRankSum=1.254;QD=28.89;ReadPosRankSum=-2.661;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|33),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 987253 . C T 635.19 . AC=4;AF=0.0333;AN=120;BaseQRankSum=0.926;DP=2121;Dels=0.00;FS=5.063;HRun=0;HaplotypeScore=1.9053;InbreedingCoeff=-0.0356;MQ=53.70;MQ0=0;MQRankSum=0.039;QD=7.22;ReadPosRankSum=-2.083;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|33),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 987311 . T C 44.81 . AC=8;AF=0.0667;AN=120;BaseQRankSum=-7.260;DP=1543;Dels=0.00;FS=87.443;HRun=3;HaplotypeScore=1.4824;InbreedingCoeff=0.0204;MQ=46.77;MQ0=0;MQRankSum=0.941;QD=0.41;ReadPosRankSum=-2.570;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|33),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 987341 . C T 217.82 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.265;DP=1310;Dels=0.00;FS=10.232;HRun=0;HaplotypeScore=1.1876;InbreedingCoeff=-0.0145;MQ=48.15;MQ0=0;MQRankSum=2.296;QD=7.03;ReadPosRankSum=0.839;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|33),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 987342 . G A 259.15 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-4.057;DP=1247;Dels=0.00;FS=1.217;HRun=0;HaplotypeScore=1.1837;InbreedingCoeff=-0.0234;MQ=48.33;MQ0=0;MQRankSum=1.716;QD=5.76;ReadPosRankSum=0.808;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|33),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 987436 . A G 63.69 . AC=9;AF=0.0865;AN=104;BaseQRankSum=2.297;DP=458;Dels=0.00;FS=20.190;HRun=0;HaplotypeScore=0.9717;InbreedingCoeff=-0.0835;MQ=56.48;MQ0=0;MQRankSum=-5.343;QD=0.49;ReadPosRankSum=-3.854;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|33),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 987670 . T G 1282.33 . AC=44;AF=0.846;AN=52;BaseQRankSum=-0.078;DP=47;Dels=0.00;FS=6.479;HRun=0;HaplotypeScore=0.0222;InbreedingCoeff=0.1213;MQ=59.01;MQ0=0;MQRankSum=-1.300;QD=30.53;ReadPosRankSum=0.391;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|33),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 988932 . G C 36554.37 . AC=79;AF=0.7596;AN=104;BaseQRankSum=-0.433;DP=1556;Dels=0.00;FS=2.118;HRun=3;HaplotypeScore=0.6684;InbreedingCoeff=0.1833;MQ=57.12;MQ0=0;MQRankSum=-1.108;QD=26.28;ReadPosRankSum=-0.844;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|33),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 989423 . G A 1179.93 . AC=6;AF=0.0500;AN=120;BaseQRankSum=-0.586;DP=696;Dels=0.00;FS=1.039;HRun=0;HaplotypeScore=0.3917;InbreedingCoeff=0.5564;MQ=58.43;MQ0=0;MQRankSum=0.139;QD=23.14;ReadPosRankSum=-0.143;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|34),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|1),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 989461 . G A 47.93 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.647;DP=465;Dels=0.00;FS=5.710;HRun=0;HaplotypeScore=0.2114;InbreedingCoeff=-0.0294;MQ=58.14;MQ0=0;MQRankSum=-0.206;QD=5.99;ReadPosRankSum=1.402;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|34),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|1),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 989500 . G A 537.75 . AC=8;AF=0.0678;AN=118;BaseQRankSum=0.771;DP=317;Dels=0.00;FS=1.526;HRun=1;HaplotypeScore=0.4247;InbreedingCoeff=0.2291;MQ=57.86;MQ0=0;MQRankSum=1.486;QD=19.21;ReadPosRankSum=-0.692;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|34),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|1),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 +chr1 989811 . C A 1223.0 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-3.075;DP=1715;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.1951;InbreedingCoeff=-0.0226;MQ=58.98;MQ0=0;MQRankSum=0.887;QD=11.87;ReadPosRankSum=-0.484;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|34),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|1),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1 +chr1 989847 . C T 704.05 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.110;DP=2200;Dels=0.00;FS=6.860;HRun=1;HaplotypeScore=1.7382;InbreedingCoeff=-0.0131;MQ=58.83;MQ0=0;MQRankSum=-0.133;QD=10.67;ReadPosRankSum=0.559;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|2),SYNONYMOUS_CODING(LOW|SILENT|Ctg/Ttg|L1966|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,synonymous_variant|Ctg/Ttg|L|ENSG00000188157|AGRN|ENST00000379370|35/36|||1966/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|2/3||||retained_intron|1 +chr1 990213 . C T 965.08 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.966;DP=1603;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.3432;InbreedingCoeff=-0.0258;MQ=58.45;MQ0=0;MQRankSum=0.239;QD=15.57;ReadPosRankSum=-0.735;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P1997L|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,missense_variant|cCg/cTg|P/L|ENSG00000188157|AGRN|ENST00000379370|36/36|possibly_damaging(0.612)|deleterious(0.04)|1997/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 +chr1 990280 . C T 40484.94 . AC=89;AF=0.7672;AN=116;BaseQRankSum=-1.914;DP=1861;Dels=0.00;FS=0.408;HRun=0;HaplotypeScore=1.6608;InbreedingCoeff=-0.0422;MQ=58.21;MQ0=0;MQRankSum=1.964;QD=23.35;ReadPosRankSum=-2.688;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D2019|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,synonymous_variant|gaC/gaT|D|ENSG00000188157|AGRN|ENST00000379370|36/36|||2019/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 +chr1 990380 . C T 8429.21 . AC=25;AF=0.2119;AN=118;BaseQRankSum=-1.731;DP=1638;Dels=0.00;FS=19.219;HRun=0;HaplotypeScore=0.9542;InbreedingCoeff=0.0822;MQ=58.01;MQ0=0;MQRankSum=-0.121;QD=14.79;ReadPosRankSum=1.180;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 +chr1 990417 . T C 8344.97 . AC=27;AF=0.2328;AN=116;BaseQRankSum=-1.168;DP=1150;Dels=0.00;FS=1.555;HRun=0;HaplotypeScore=0.4386;InbreedingCoeff=0.0986;MQ=57.95;MQ0=0;MQRankSum=-0.663;QD=16.33;ReadPosRankSum=-1.059;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),EXON(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|1),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|1/2||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 +chr1 990517 . C T 9650.24 . AC=75;AF=0.798;AN=94;BaseQRankSum=1.624;DP=348;Dels=0.00;FS=0.641;HRun=0;HaplotypeScore=0.4773;InbreedingCoeff=0.1734;MQ=57.98;MQ0=0;MQRankSum=0.522;QD=30.54;ReadPosRankSum=1.100;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),EXON(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|1),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|1/2||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 +chr1 990557 . C T 59.21 . AC=1;AF=0.011;AN=90;BaseQRankSum=2.271;DP=279;Dels=0.00;FS=2.553;HRun=0;HaplotypeScore=0.3165;InbreedingCoeff=-0.0942;MQ=57.30;MQ0=0;MQRankSum=0.765;QD=8.46;ReadPosRankSum=-0.239;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),EXON(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|1),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|1/2||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 +chr1 990773 . C T 25625.67 . AC=51;AF=0.689;AN=74;BaseQRankSum=-0.671;DP=1023;Dels=0.00;FS=8.374;HRun=0;HaplotypeScore=0.7685;InbreedingCoeff=0.2919;MQ=57.82;MQ0=0;MQRankSum=0.992;QD=27.26;ReadPosRankSum=1.535;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),INTRON(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|1),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=intron_variant&nc_transcript_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 +chr1 990806 . G A 25266.48 . AC=55;AF=0.764;AN=72;BaseQRankSum=-2.112;DP=1071;Dels=0.00;FS=2.830;HRun=0;HaplotypeScore=0.8208;InbreedingCoeff=0.2300;MQ=58.13;MQ0=0;MQRankSum=-0.461;QD=25.63;ReadPosRankSum=0.166;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),INTRON(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|1),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=intron_variant&nc_transcript_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 +chr1 990839 . C T 1668.45 . AC=2;AF=0.029;AN=70;BaseQRankSum=1.196;DP=1096;Dels=0.00;FS=1.633;HRun=2;HaplotypeScore=0.6892;InbreedingCoeff=-0.0818;MQ=58.31;MQ0=0;MQRankSum=1.390;QD=12.27;ReadPosRankSum=-2.018;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),INTRON(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|1),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=intron_variant&nc_transcript_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 +chr1 990943 . G C 216.41 . AC=1;AF=0.019;AN=52;BaseQRankSum=-0.081;DP=536;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6541;InbreedingCoeff=-0.0441;MQ=58.64;MQ0=0;MQRankSum=-0.296;QD=10.31;ReadPosRankSum=-0.366;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),INTRON(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|1),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=intron_variant&nc_transcript_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 +chr1 990984 . G A 3835.45 . AC=33;AF=0.786;AN=42;BaseQRankSum=-0.791;DP=131;Dels=0.00;FS=7.195;HRun=0;HaplotypeScore=0.1328;InbreedingCoeff=0.2454;MQ=58.69;MQ0=0;MQRankSum=-2.076;QD=32.78;ReadPosRankSum=-1.157;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),INTRON(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|1),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=intron_variant&nc_transcript_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 +chr1 1102069 . A G 744.18 . AC=36;AF=0.900;AN=40;BaseQRankSum=-0.783;DP=29;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0800;InbreedingCoeff=0.1040;MQ=56.74;MQ0=0;MQRankSum=0.679;QD=32.36;ReadPosRankSum=1.410;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=upstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1102296 . G A 783.47 . AC=4;AF=0.0357;AN=112;BaseQRankSum=-0.578;DP=498;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.2286;InbreedingCoeff=0.2474;MQ=55.59;MQ0=0;MQRankSum=0.771;QD=18.22;ReadPosRankSum=0.212;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=upstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1102360 . T G 216.82 . AC=16;AF=0.1379;AN=116;BaseQRankSum=-10.289;DP=1092;Dels=0.00;FS=25.292;HRun=4;HaplotypeScore=1.6139;InbreedingCoeff=-0.2074;MQ=53.49;MQ0=0;MQRankSum=2.414;QD=0.71;ReadPosRankSum=7.754;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=upstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1102563 . G A 1052.38 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.152;DP=4513;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=2.2999;InbreedingCoeff=-0.0179;MQ=56.60;MQ0=0;MQRankSum=2.502;QD=14.03;ReadPosRankSum=-0.938;EFF=EXON(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|1),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000207730|MIR200B|ENST00000384997|1/1||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1102657 . C T 464.41 . AC=2;AF=0.0169;AN=118;BaseQRankSum=0.429;DP=1409;Dels=0.00;FS=2.023;HRun=0;HaplotypeScore=1.5640;InbreedingCoeff=-0.0230;MQ=53.83;MQ0=0;MQRankSum=0.937;QD=12.90;ReadPosRankSum=0.639;EFF=DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1102674 . G A 964.97 . AC=2;AF=0.0172;AN=116;BaseQRankSum=0.337;DP=1131;Dels=0.00;FS=1.614;HRun=0;HaplotypeScore=1.4083;InbreedingCoeff=-0.0229;MQ=55.34;MQ0=0;MQRankSum=1.906;QD=12.21;ReadPosRankSum=1.395;EFF=DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1102717 . C T 175.26 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.432;DP=649;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.6822;InbreedingCoeff=-0.0345;MQ=56.83;MQ0=0;MQRankSum=1.021;QD=9.22;ReadPosRankSum=1.763;EFF=DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1103049 . C T 201.32 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.756;DP=465;Dels=0.00;FS=3.302;HRun=0;HaplotypeScore=0.3840;InbreedingCoeff=-0.0481;MQ=57.23;MQ0=0;MQRankSum=0.101;QD=9.15;ReadPosRankSum=-0.265;EFF=DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1103150 . G A 13784.17 . AC=26;AF=0.2281;AN=114;BaseQRankSum=2.288;DP=1923;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.8331;InbreedingCoeff=0.0633;MQ=56.95;MQ0=0;MQRankSum=6.711;QD=19.50;ReadPosRankSum=1.282;EFF=DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1103231 . C G 920.65 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.613;DP=4071;Dels=0.00;FS=1.295;HRun=0;HaplotypeScore=2.5754;InbreedingCoeff=-0.0143;MQ=57.14;MQ0=0;MQRankSum=0.020;QD=9.59;ReadPosRankSum=1.029;EFF=DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1103423 . GA G 310.03 . AC=11;AF=0.0932;AN=118;BaseQRankSum=3.186;DP=1367;FS=7.032;HRun=1;HaplotypeScore=112.5777;InbreedingCoeff=-0.1319;MQ=56.78;MQ0=0;MQRankSum=-1.054;QD=1.13;ReadPosRankSum=-12.462;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1103424 . A G 291.86 . AC=11;AF=0.0932;AN=118;BaseQRankSum=-4.363;DP=1367;Dels=0.01;FS=12.595;HRun=3;HaplotypeScore=1.1874;InbreedingCoeff=-0.1339;MQ=56.78;MQ0=0;MQRankSum=-0.884;QD=0.96;ReadPosRankSum=-10.797;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1103436 . C T 253.89 . AC=1;AF=0.0094;AN=106;BaseQRankSum=1.346;DP=1176;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.0420;InbreedingCoeff=-0.0281;MQ=56.38;MQ0=0;MQRankSum=-1.314;QD=7.25;ReadPosRankSum=-3.184;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1103471 . C T 3129.31 . AC=41;AF=0.3596;AN=114;BaseQRankSum=7.298;DP=802;Dels=0.00;FS=25.877;HRun=0;HaplotypeScore=0.4705;InbreedingCoeff=-0.6199;MQ=56.31;MQ0=0;MQRankSum=4.884;QD=4.53;ReadPosRankSum=-15.448;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1103479 . G C 231.39 . AC=9;AF=0.0804;AN=112;BaseQRankSum=-1.555;DP=715;Dels=0.00;FS=4.645;HRun=2;HaplotypeScore=0.4522;InbreedingCoeff=-0.1256;MQ=56.12;MQ0=0;MQRankSum=1.010;QD=2.22;ReadPosRankSum=-8.435;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1103537 . C T 42.98 . AC=4;AF=0.0364;AN=110;BaseQRankSum=-0.058;DP=526;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3527;InbreedingCoeff=0.3068;MQ=56.99;MQ0=0;MQRankSum=1.015;QD=14.33;ReadPosRankSum=0.313;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1103542 . T C 13387.36 . AC=101;AF=0.9182;AN=110;BaseQRankSum=2.524;DP=466;Dels=0.00;FS=6.051;HRun=2;HaplotypeScore=0.2267;InbreedingCoeff=0.0050;MQ=57.11;MQ0=0;MQRankSum=1.977;QD=29.29;ReadPosRankSum=-2.662;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1103690 . T TCA 4213.79 . AC=60;AF=0.909;AN=66;BaseQRankSum=-2.219;DP=87;FS=4.960;HRun=0;HaplotypeScore=8.2650;InbreedingCoeff=0.2982;MQ=60.22;MQ0=0;MQRankSum=-0.386;QD=52.02;ReadPosRankSum=-1.623;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1103958 . T G 972.47 . AC=38;AF=0.905;AN=42;BaseQRankSum=0.039;DP=33;Dels=0.00;FS=2.722;HRun=0;HaplotypeScore=0.0376;InbreedingCoeff=0.1168;MQ=59.30;MQ0=0;MQRankSum=0.973;QD=31.37;ReadPosRankSum=-0.195;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 +chr1 1104406 . C CCAGA 1690.42 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-4.911;DP=3757;FS=4.900;HRun=0;HaplotypeScore=321.6033;InbreedingCoeff=-0.0086;MQ=57.98;MQ0=0;MQRankSum=-8.640;QD=27.71;ReadPosRankSum=0.563;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),EXON(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|1),UPSTREAM(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|),UPSTREAM(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant&feature_elongation|||ENSG00000198976|MIR429|ENST00000362106|1/1||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1 +chr1 1104418 . A C 1306.94 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.348;DP=3836;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.7746;InbreedingCoeff=-0.0084;MQ=57.98;MQ0=0;MQRankSum=-0.429;QD=15.38;ReadPosRankSum=1.041;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),EXON(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|1),UPSTREAM(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|),UPSTREAM(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000198976|MIR429|ENST00000362106|1/1||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1 +chr1 1104584 . T C 2225.46 . AC=7;AF=0.0583;AN=120;BaseQRankSum=-1.668;DP=1142;Dels=0.00;FS=2.698;HRun=0;HaplotypeScore=0.5785;InbreedingCoeff=0.5329;MQ=58.24;MQ0=0;MQRankSum=1.566;QD=23.93;ReadPosRankSum=1.120;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|),UPSTREAM(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1,downstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1 +chr1 1104624 . C T 353.09 . AC=2;AF=0.0167;AN=120;BaseQRankSum=1.668;DP=653;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.6949;InbreedingCoeff=-0.0240;MQ=57.96;MQ0=0;MQRankSum=0.018;QD=15.35;ReadPosRankSum=0.448;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|),UPSTREAM(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1,downstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1 +chr1 1115086 . C T 1455.37 . AC=1;AF=0.0086;AN=116;BaseQRankSum=-2.311;DP=1823;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.0613;InbreedingCoeff=-0.0319;MQ=58.20;MQ0=0;MQRankSum=0.635;QD=14.13;ReadPosRankSum=-1.886;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G56|673|TTLL10|protein_coding|CODING|ENST00000379289|),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G56|673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),UTR_5_PRIME(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|1);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,synonymous_variant|ggC/ggT|G|ENSG00000162571|TTLL10|ENST00000379289|5/16|||56/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,synonymous_variant|ggC/ggT|G|ENSG00000162571|TTLL10|ENST00000379290|5/16|||56/673|protein_coding|1,5_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|1/9|||-/404|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1 +chr1 1115187 . C T 128.35 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.636;DP=678;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5936;InbreedingCoeff=-0.0317;MQ=57.89;MQ0=0;MQRankSum=0.815;QD=14.26;ReadPosRankSum=0.228;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|5),START_GAINED(LOW||||404|TTLL10|protein_coding|CODING|ENST00000379288|1),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,5_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|1/9|||-/404|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1 +chr1 1115210 . G A 3468.51 . AC=23;AF=0.1983;AN=116;BaseQRankSum=-0.114;DP=539;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6710;InbreedingCoeff=0.0851;MQ=58.05;MQ0=0;MQRankSum=-0.518;QD=16.84;ReadPosRankSum=-1.559;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|5),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),UTR_5_PRIME(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|1);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,5_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|1/9|||-/404|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1 +chr1 1115213 . G T 2687.22 . AC=21;AF=0.1810;AN=116;BaseQRankSum=0.184;DP=530;Dels=0.00;FS=4.737;HRun=1;HaplotypeScore=0.7398;InbreedingCoeff=0.1254;MQ=58.19;MQ0=0;MQRankSum=0.107;QD=15.10;ReadPosRankSum=-1.774;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|5),START_GAINED(LOW||||404|TTLL10|protein_coding|CODING|ENST00000379288|1),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,5_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|1/9|||-/404|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1 +chr1 1115222 . C T 45.39 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-0.693;DP=471;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3240;InbreedingCoeff=-0.0359;MQ=58.17;MQ0=0;MQRankSum=0.038;QD=5.67;ReadPosRankSum=-1.442;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|5),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),UTR_5_PRIME(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|1);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,5_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|1/9|||-/404|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1 +chr1 1115379 . G A 616.46 . AC=5;AF=0.0417;AN=120;BaseQRankSum=0.553;DP=584;Dels=0.00;FS=2.233;HRun=0;HaplotypeScore=0.3425;InbreedingCoeff=-0.0782;MQ=58.20;MQ0=0;MQRankSum=1.750;QD=9.94;ReadPosRankSum=0.385;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|1),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|5),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1 +chr1 1115415 . C T 177.42 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-1.248;DP=752;Dels=0.00;FS=3.560;HRun=0;HaplotypeScore=0.6217;InbreedingCoeff=-0.0287;MQ=58.39;MQ0=0;MQRankSum=1.308;QD=8.06;ReadPosRankSum=-0.853;EFF=SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G67|673|TTLL10|protein_coding|CODING|ENST00000379289|),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G67|673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),UTR_5_PRIME(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|2);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,splice_region_variant&synonymous_variant|ggC/ggT|G|ENSG00000162571|TTLL10|ENST00000379289|6/16|||67/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,splice_region_variant&synonymous_variant|ggC/ggT|G|ENSG00000162571|TTLL10|ENST00000379290|6/16|||67/673|protein_coding|1,splice_region_variant&5_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|2/9|||-/404|protein_coding|1 +chr1 1115461 . C T 331.7 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.166;DP=949;Dels=0.00;FS=1.152;HRun=0;HaplotypeScore=0.9760;InbreedingCoeff=-0.0264;MQ=58.65;MQ0=0;MQRankSum=1.177;QD=12.76;ReadPosRankSum=1.958;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgg/Tgg|R10W|404|TTLL10|protein_coding|CODING|ENST00000379288|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgg/Tgg|R83W|673|TTLL10|protein_coding|CODING|ENST00000379289|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgg/Tgg|R83W|673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,missense_variant|Cgg/Tgg|R/W|ENSG00000162571|TTLL10|ENST00000379289|6/16|benign(0.28)|tolerated(0.08)|83/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,missense_variant|Cgg/Tgg|R/W|ENSG00000162571|TTLL10|ENST00000379290|6/16|benign(0.28)|tolerated(0.08)|83/673|protein_coding|1,missense_variant|Cgg/Tgg|R/W|ENSG00000162571|TTLL10|ENST00000379288|2/9|benign(0.365)|deleterious(0.03)|10/404|protein_coding|1 +chr1 1115503 . T C 1171.08 . AC=4;AF=0.0333;AN=120;BaseQRankSum=1.533;DP=1103;Dels=0.00;FS=7.100;HRun=1;HaplotypeScore=0.5750;InbreedingCoeff=-0.0426;MQ=59.00;MQ0=0;MQRankSum=1.371;QD=12.59;ReadPosRankSum=-1.312;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tgt/Cgt|C24R|404|TTLL10|protein_coding|CODING|ENST00000379288|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tgt/Cgt|C97R|673|TTLL10|protein_coding|CODING|ENST00000379289|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tgt/Cgt|C97R|673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,missense_variant|Tgt/Cgt|C/R|ENSG00000162571|TTLL10|ENST00000379289|6/16|benign(0.004)|tolerated(0.53)|97/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,missense_variant|Tgt/Cgt|C/R|ENSG00000162571|TTLL10|ENST00000379290|6/16|benign(0.004)|tolerated(0.53)|97/673|protein_coding|1,missense_variant|Tgt/Cgt|C/R|ENSG00000162571|TTLL10|ENST00000379288|2/9|benign(0.016)|tolerated(0.27)|24/404|protein_coding|1 +chr1 1115601 . C T 368.82 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-0.437;DP=1078;Dels=0.00;FS=12.205;HRun=0;HaplotypeScore=0.6665;InbreedingCoeff=-0.0165;MQ=58.97;MQ0=0;MQRankSum=0.473;QD=12.29;ReadPosRankSum=2.503;EFF=SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N129|673|TTLL10|protein_coding|CODING|ENST00000379289|),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N129|673|TTLL10|protein_coding|CODING|ENST00000379290|),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N56|404|TTLL10|protein_coding|CODING|ENST00000379288|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,synonymous_variant|aaC/aaT|N|ENSG00000162571|TTLL10|ENST00000379289|6/16|||129/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,synonymous_variant|aaC/aaT|N|ENSG00000162571|TTLL10|ENST00000379290|6/16|||129/673|protein_coding|1,synonymous_variant|aaC/aaT|N|ENSG00000162571|TTLL10|ENST00000379288|2/9|||56/404|protein_coding|1 +chr1 1115605 . G T 78.84 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.557;DP=1031;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6145;InbreedingCoeff=-0.0171;MQ=58.95;MQ0=0;MQRankSum=0.835;QD=4.93;ReadPosRankSum=0.136;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Tcc|A131S|673|TTLL10|protein_coding|CODING|ENST00000379289|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Tcc|A131S|673|TTLL10|protein_coding|CODING|ENST00000379290|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Tcc|A58S|404|TTLL10|protein_coding|CODING|ENST00000379288|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,missense_variant|Gcc/Tcc|A/S|ENSG00000162571|TTLL10|ENST00000379289|6/16|benign(0.021)|tolerated(0.07)|131/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,missense_variant|Gcc/Tcc|A/S|ENSG00000162571|TTLL10|ENST00000379290|6/16|benign(0.021)|tolerated(0.07)|131/673|protein_coding|1,missense_variant|Gcc/Tcc|A/S|ENSG00000162571|TTLL10|ENST00000379288|2/9|benign(0.087)|tolerated(0.07)|58/404|protein_coding|1 +chr1 1115739 . C T 563.52 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-0.563;DP=900;Dels=0.00;FS=2.949;HRun=0;HaplotypeScore=0.6729;InbreedingCoeff=-0.0307;MQ=58.26;MQ0=0;MQRankSum=1.624;QD=17.61;ReadPosRankSum=1.518;EFF=INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|2),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|6),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|6),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1115840 . AC A 723.63 . AC=2;AF=0.0175;AN=114;BaseQRankSum=1.500;DP=1665;FS=3.486;HRun=5;HaplotypeScore=137.9849;InbreedingCoeff=-0.0404;MQ=57.81;MQ0=0;MQRankSum=1.153;QD=10.19;ReadPosRankSum=1.601;EFF=INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|2),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|6),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|6),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1115994 . G A 10204.82 . AC=24;AF=0.2000;AN=120;BaseQRankSum=-0.160;DP=1696;Dels=0.00;FS=0.223;HRun=0;HaplotypeScore=1.0682;InbreedingCoeff=0.0308;MQ=58.03;MQ0=0;MQRankSum=0.094;QD=16.57;ReadPosRankSum=1.260;EFF=EXON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|1),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|3),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|7),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|7),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|1),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|1/5||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1116005 . C T 537.05 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.435;DP=1666;Dels=0.00;FS=14.791;HRun=0;HaplotypeScore=1.1478;InbreedingCoeff=-0.0145;MQ=58.06;MQ0=0;MQRankSum=1.799;QD=13.43;ReadPosRankSum=-0.495;EFF=EXON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|1),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|3),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|7),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|7),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|1),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|1/5||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1116231 . T C 4814.31 . AC=14;AF=0.1167;AN=120;BaseQRankSum=2.445;DP=1389;Dels=0.00;FS=6.355;HRun=2;HaplotypeScore=1.7202;InbreedingCoeff=0.1639;MQ=56.15;MQ0=0;MQRankSum=1.206;QD=15.28;ReadPosRankSum=0.519;EFF=EXON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|1),EXON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|2),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTc/gCc|V176A|404|TTLL10|protein_coding|CODING|ENST00000379288|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTc/gCc|V249A|673|TTLL10|protein_coding|CODING|ENST00000379289|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTc/gCc|V249A|673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,missense_variant|gTc/gCc|V/A|ENSG00000162571|TTLL10|ENST00000379289|8/16|benign(0)|tolerated(1)|249/673|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|1/5||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000514695|2/2||||retained_intron|1,missense_variant|gTc/gCc|V/A|ENSG00000162571|TTLL10|ENST00000379290|8/16|benign(0)|tolerated(1)|249/673|protein_coding|1,missense_variant|gTc/gCc|V/A|ENSG00000162571|TTLL10|ENST00000379288|4/9|benign(0)|tolerated(1)|176/404|protein_coding|1 +chr1 1116311 . A G 3493.8 . AC=48;AF=0.4706;AN=102;BaseQRankSum=7.547;DP=286;Dels=0.01;FS=2.211;HRun=0;HaplotypeScore=0.9639;InbreedingCoeff=0.0983;MQ=52.73;MQ0=0;MQRankSum=0.694;QD=18.89;ReadPosRankSum=0.096;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|4),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|8),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|1),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1117111 . T C 797.3 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.679;DP=2103;Dels=0.00;FS=1.613;HRun=4;HaplotypeScore=1.6462;InbreedingCoeff=-0.0099;MQ=57.76;MQ0=0;MQRankSum=1.258;QD=16.61;ReadPosRankSum=-0.652;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|4),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|8),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|1),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1117200 . A ACTCAG 2202.05 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-4.680;DP=3092;FS=21.350;HRun=0;HaplotypeScore=287.1827;InbreedingCoeff=-0.0395;MQ=57.35;MQ0=0;MQRankSum=-7.883;QD=28.60;ReadPosRankSum=-0.894;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|9),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|9),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|2),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,splice_region_variant&intron_variant&feature_elongation|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,splice_region_variant&intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,splice_region_variant&intron_variant&feature_elongation|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,splice_region_variant&intron_variant&feature_elongation|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1117398 . A G 8833.57 . AC=60;AF=0.5172;AN=116;BaseQRankSum=3.163;DP=557;Dels=0.00;FS=2.617;HRun=0;HaplotypeScore=0.4621;InbreedingCoeff=0.0835;MQ=57.86;MQ0=0;MQRankSum=1.868;QD=22.25;ReadPosRankSum=0.084;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|9),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|9),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|2),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1117486 . A G 1272.34 . AC=17;AF=0.1441;AN=118;BaseQRankSum=-1.118;DP=292;Dels=0.00;FS=2.091;HRun=0;HaplotypeScore=0.1964;InbreedingCoeff=-0.1365;MQ=57.43;MQ0=0;MQRankSum=0.534;QD=14.14;ReadPosRankSum=0.569;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|9),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|9),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|2),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1117529 . A C 317.04 . AC=5;AF=0.0424;AN=118;BaseQRankSum=-1.360;DP=362;Dels=0.00;FS=1.548;HRun=1;HaplotypeScore=0.4341;InbreedingCoeff=-0.0846;MQ=57.60;MQ0=0;MQRankSum=0.707;QD=9.61;ReadPosRankSum=0.810;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|9),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|9),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|2),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1117709 . G A 2986.58 . AC=4;AF=0.0333;AN=120;BaseQRankSum=1.592;DP=3059;Dels=0.00;FS=8.817;HRun=1;HaplotypeScore=1.5659;InbreedingCoeff=-0.0345;MQ=59.00;MQ0=0;MQRankSum=-1.150;QD=12.82;ReadPosRankSum=-4.568;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|9),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|9),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|2),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1117795 . C G 1423.49 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.182;DP=4738;Dels=0.00;FS=1.073;HRun=1;HaplotypeScore=2.2289;InbreedingCoeff=-0.0084;MQ=59.08;MQ0=0;MQRankSum=-0.250;QD=14.53;ReadPosRankSum=2.714;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),EXON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|3),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|caC/caG|H222Q|404|TTLL10|protein_coding|CODING|ENST00000379288|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|caC/caG|H295Q|673|TTLL10|protein_coding|CODING|ENST00000379289|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|caC/caG|H295Q|673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,missense_variant|caC/caG|H/Q|ENSG00000162571|TTLL10|ENST00000379289|10/16|benign(0.046)|tolerated(0.21)|295/673|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|3/5||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,missense_variant|caC/caG|H/Q|ENSG00000162571|TTLL10|ENST00000379290|10/16|benign(0.046)|tolerated(0.21)|295/673|protein_coding|1,missense_variant|caC/caG|H/Q|ENSG00000162571|TTLL10|ENST00000379288|6/9|benign(0.085)|tolerated(0.23)|222/404|protein_coding|1 +chr1 1118119 . G A 94.5 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.954;DP=605;Dels=0.00;FS=6.112;HRun=0;HaplotypeScore=0.5514;InbreedingCoeff=-0.0360;MQ=57.50;MQ0=0;MQRankSum=0.026;QD=15.75;ReadPosRankSum=-0.231;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|6),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|10),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|10),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|3),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1118157 . C T 629.37 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.602;DP=775;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.4619;InbreedingCoeff=-0.0286;MQ=57.49;MQ0=0;MQRankSum=1.353;QD=15.73;ReadPosRankSum=-1.120;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|6),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|10),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|10),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|3),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1118212 . T C 16130.03 . AC=48;AF=0.4068;AN=118;BaseQRankSum=1.657;DP=1395;Dels=0.00;FS=12.889;HRun=1;HaplotypeScore=1.7343;InbreedingCoeff=0.1338;MQ=57.23;MQ0=0;MQRankSum=3.897;QD=20.34;ReadPosRankSum=-0.066;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|6),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|10),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|10),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|3),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1118244 . C T 1034.91 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.695;DP=2048;Dels=0.00;FS=2.342;HRun=0;HaplotypeScore=1.2560;InbreedingCoeff=-0.0165;MQ=57.74;MQ0=0;MQRankSum=0.426;QD=10.78;ReadPosRankSum=-0.861;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|6),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|10),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|10),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|3),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1118275 . C T 6047.77 . AC=9;AF=0.0750;AN=120;BaseQRankSum=-1.144;DP=2721;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.3228;InbreedingCoeff=-0.0826;MQ=57.77;MQ0=0;MQRankSum=4.599;QD=12.68;ReadPosRankSum=1.658;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),EXON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|4),SYNONYMOUS_CODING(LOW|SILENT|tgC/tgT|C239|404|TTLL10|protein_coding|CODING|ENST00000379288|),SYNONYMOUS_CODING(LOW|SILENT|tgC/tgT|C312|673|TTLL10|protein_coding|CODING|ENST00000379289|),SYNONYMOUS_CODING(LOW|SILENT|tgC/tgT|C312|673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,synonymous_variant|tgC/tgT|C|ENSG00000162571|TTLL10|ENST00000379289|11/16|||312/673|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|4/5||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,synonymous_variant|tgC/tgT|C|ENSG00000162571|TTLL10|ENST00000379290|11/16|||312/673|protein_coding|1,synonymous_variant|tgC/tgT|C|ENSG00000162571|TTLL10|ENST00000379288|7/9|||239/404|protein_coding|1 +chr1 1118491 . A G 315.28 . AC=4;AF=0.0339;AN=118;BaseQRankSum=-1.758;DP=1080;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=1.0651;InbreedingCoeff=-0.0464;MQ=56.58;MQ0=0;MQRankSum=0.706;QD=10.87;ReadPosRankSum=0.829;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|7),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|11),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|11),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|4),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1118522 . G A 231.29 . AC=2;AF=0.0169;AN=118;BaseQRankSum=0.115;DP=734;Dels=0.00;FS=22.046;HRun=0;HaplotypeScore=0.5763;InbreedingCoeff=-0.0479;MQ=55.98;MQ0=0;MQRankSum=0.916;QD=10.51;ReadPosRankSum=-2.177;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|7),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|11),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|11),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|4),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1118529 . G C 35.12 . AC=5;AF=0.0431;AN=116;BaseQRankSum=-3.621;DP=699;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.6631;InbreedingCoeff=0.1223;MQ=55.74;MQ0=0;MQRankSum=-0.031;QD=1.60;ReadPosRankSum=-2.064;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|7),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|11),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|11),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|4),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1118560 . C T 120.5 . AC=4;AF=0.0339;AN=118;BaseQRankSum=0.441;DP=502;Dels=0.00;FS=2.806;HRun=0;HaplotypeScore=0.5435;InbreedingCoeff=0.0953;MQ=53.92;MQ0=0;MQRankSum=2.564;QD=9.27;ReadPosRankSum=-0.278;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|7),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|11),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|11),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|4),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1118578 . G A 921.34 . AC=9;AF=0.0804;AN=112;BaseQRankSum=-0.517;DP=400;Dels=0.00;FS=2.546;HRun=1;HaplotypeScore=0.3066;InbreedingCoeff=-0.0266;MQ=53.56;MQ0=0;MQRankSum=0.375;QD=14.86;ReadPosRankSum=2.525;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|7),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|11),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|11),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|4),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1119080 . T C 90.74 . AC=1;AF=0.0086;AN=116;BaseQRankSum=0.742;DP=369;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.3252;InbreedingCoeff=-0.0596;MQ=58.29;MQ0=0;MQRankSum=0.380;QD=12.96;ReadPosRankSum=-0.100;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|7),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|11),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|11),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|4),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1119399 . C T 1267.07 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.885;DP=8859;DS;Dels=0.00;FS=6.806;HRun=0;HaplotypeScore=4.5609;InbreedingCoeff=-0.0084;MQ=59.04;MQ0=0;MQRankSum=-1.312;QD=11.73;ReadPosRankSum=1.342;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H323|404|TTLL10|protein_coding|CODING|ENST00000379288|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H396|673|TTLL10|protein_coding|CODING|ENST00000379289|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H396|673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,synonymous_variant|caC/caT|H|ENSG00000162571|TTLL10|ENST00000379289|12/16|||396/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,synonymous_variant|caC/caT|H|ENSG00000162571|TTLL10|ENST00000379290|12/16|||396/673|protein_coding|1,synonymous_variant|caC/caT|H|ENSG00000162571|TTLL10|ENST00000379288|8/9|||323/404|protein_coding|1 +chr1 1119543 . G C 392.87 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.504;DP=2292;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.8936;InbreedingCoeff=-0.0096;MQ=59.06;MQ0=0;MQRankSum=0.601;QD=13.55;ReadPosRankSum=-1.427;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1119546 . G T 1327.72 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.808;DP=2166;Dels=0.00;FS=1.426;HRun=1;HaplotypeScore=1.8266;InbreedingCoeff=-0.0272;MQ=59.08;MQ0=0;MQRankSum=-0.008;QD=14.59;ReadPosRankSum=-1.147;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1119657 . G C 5454.82 . AC=49;AF=0.4375;AN=112;BaseQRankSum=3.181;DP=432;Dels=0.00;FS=3.269;HRun=3;HaplotypeScore=0.4863;InbreedingCoeff=0.1117;MQ=58.71;MQ0=0;MQRankSum=1.349;QD=20.58;ReadPosRankSum=-0.252;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1119796 . C T 91.64 . AC=5;AF=0.078;AN=64;BaseQRankSum=-0.197;DP=63;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.1159;InbreedingCoeff=-0.0863;MQ=58.22;MQ0=0;MQRankSum=0.250;QD=9.16;ReadPosRankSum=-0.408;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1119858 . C T 125.02 . AC=7;AF=0.130;AN=54;BaseQRankSum=-0.015;DP=50;Dels=0.00;FS=4.112;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=0.0152;MQ=56.27;MQ0=0;MQRankSum=0.412;QD=13.89;ReadPosRankSum=1.205;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1120091 . G A 172.79 . AC=11;AF=0.145;AN=76;BaseQRankSum=0.184;DP=127;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1032;InbreedingCoeff=0.0586;MQ=42.08;MQ0=0;MQRankSum=-1.381;QD=4.80;ReadPosRankSum=1.301;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12);CSQ=upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1120307 . G A 5288.32 . AC=23;AF=0.235;AN=98;BaseQRankSum=-0.512;DP=784;Dels=0.00;FS=3.693;HRun=0;HaplotypeScore=0.5879;InbreedingCoeff=0.2421;MQ=53.35;MQ0=0;MQRankSum=3.646;QD=18.62;ReadPosRankSum=1.917;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12);CSQ=upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1120337 . G A 33.77 . AC=2;AF=0.0167;AN=120;BaseQRankSum=0.146;DP=1640;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.8805;InbreedingCoeff=-0.0159;MQ=54.17;MQ0=0;MQRankSum=2.540;QD=0.70;ReadPosRankSum=2.633;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12);CSQ=upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1120343 . C T 699.62 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.743;DP=1871;Dels=0.00;FS=5.662;HRun=0;HaplotypeScore=1.0425;InbreedingCoeff=-0.0085;MQ=54.11;MQ0=0;MQRankSum=0.033;QD=16.66;ReadPosRankSum=2.495;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12);CSQ=upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,splice_region_variant&intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 +chr1 1120370 . C G 708.51 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.070;DP=2729;Dels=0.00;FS=2.564;HRun=0;HaplotypeScore=1.4361;InbreedingCoeff=-0.0084;MQ=54.85;MQ0=0;MQRankSum=2.839;QD=10.12;ReadPosRankSum=1.250;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctg/Gtg|L355V|404|TTLL10|protein_coding|CODING|ENST00000379288|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctg/Gtg|L428V|673|TTLL10|protein_coding|CODING|ENST00000379289|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctg/Gtg|L428V|673|TTLL10|protein_coding|CODING|ENST00000379290|);CSQ=upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,missense_variant|Ctg/Gtg|L/V|ENSG00000162571|TTLL10|ENST00000379289|13/16|probably_damaging(0.951)|tolerated(0.12)|428/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,missense_variant|Ctg/Gtg|L/V|ENSG00000162571|TTLL10|ENST00000379290|13/16|probably_damaging(0.951)|tolerated(0.12)|428/673|protein_coding|1,missense_variant|Ctg/Gtg|L/V|ENSG00000162571|TTLL10|ENST00000379288|9/9|probably_damaging(0.999)|tolerated(0.12)|355/404|protein_coding|1 +chr1 1120377 . T A 4655.8 . AC=5;AF=0.0417;AN=120;BaseQRankSum=1.090;DP=2884;Dels=0.00;FS=2.193;HRun=1;HaplotypeScore=1.7603;InbreedingCoeff=-0.0435;MQ=55.01;MQ0=0;MQRankSum=6.538;QD=16.22;ReadPosRankSum=-0.513;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aTg/aAg|M357K|404|TTLL10|protein_coding|CODING|ENST00000379288|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aTg/aAg|M430K|673|TTLL10|protein_coding|CODING|ENST00000379289|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aTg/aAg|M430K|673|TTLL10|protein_coding|CODING|ENST00000379290|);CSQ=upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,missense_variant|aTg/aAg|M/K|ENSG00000162571|TTLL10|ENST00000379289|13/16|benign(0.181)|tolerated(0.99)|430/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,missense_variant|aTg/aAg|M/K|ENSG00000162571|TTLL10|ENST00000379290|13/16|benign(0.181)|tolerated(0.99)|430/673|protein_coding|1,missense_variant|aTg/aAg|M/K|ENSG00000162571|TTLL10|ENST00000379288|9/9|benign(0.088)|tolerated(0.91)|357/404|protein_coding|1 +chr1 1120431 . G A 10163.92 . AC=16;AF=0.1333;AN=120;BaseQRankSum=-1.606;DP=3363;Dels=0.00;FS=20.818;HRun=1;HaplotypeScore=1.5931;InbreedingCoeff=0.1346;MQ=56.00;MQ0=0;MQRankSum=8.100;QD=14.38;ReadPosRankSum=1.101;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGt/aAt|S375N|404|TTLL10|protein_coding|CODING|ENST00000379288|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGt/aAt|S448N|673|TTLL10|protein_coding|CODING|ENST00000379289|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGt/aAt|S448N|673|TTLL10|protein_coding|CODING|ENST00000379290|);CSQ=missense_variant&NMD_transcript_variant|aGt/aAt|S/N|ENSG00000162571|TTLL10|ENST00000486379|1/5|unknown(0)||6/45|nonsense_mediated_decay|1,missense_variant|aGt/aAt|S/N|ENSG00000162571|TTLL10|ENST00000379289|13/16|benign(0)|tolerated(1)|448/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,missense_variant|aGt/aAt|S/N|ENSG00000162571|TTLL10|ENST00000379290|13/16|benign(0)|tolerated(1)|448/673|protein_coding|1,missense_variant|aGt/aAt|S/N|ENSG00000162571|TTLL10|ENST00000379288|9/9|benign(0)|tolerated(1)|375/404|protein_coding|1 +chr1 1120536 . G A 3644.72 . AC=9;AF=0.0750;AN=120;BaseQRankSum=-1.719;DP=1842;Dels=0.00;FS=0.462;HRun=0;HaplotypeScore=1.6538;InbreedingCoeff=0.1586;MQ=56.93;MQ0=0;MQRankSum=7.086;QD=15.99;ReadPosRankSum=-0.320;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|13),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|13),UTR_3_PRIME(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|9);CSQ=intron_variant&NMD_transcript_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,3_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|9/9|||-/404|protein_coding|1 +chr1 1120587 . G A 207.5 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.370;DP=1099;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7829;InbreedingCoeff=-0.0101;MQ=57.36;MQ0=0;MQRankSum=0.481;QD=11.53;ReadPosRankSum=0.161;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|13),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|13),UTR_3_PRIME(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|9);CSQ=intron_variant&NMD_transcript_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,3_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|9/9|||-/404|protein_coding|1 +chr1 1120621 . T C 922.19 . AC=6;AF=0.0500;AN=120;BaseQRankSum=-0.439;DP=752;Dels=0.00;FS=2.007;HRun=0;HaplotypeScore=0.6123;InbreedingCoeff=-0.0575;MQ=57.87;MQ0=0;MQRankSum=1.573;QD=10.72;ReadPosRankSum=-1.626;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|13),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|13),UTR_3_PRIME(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|9);CSQ=intron_variant&NMD_transcript_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,3_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|9/9|||-/404|protein_coding|1 +chr1 1138735 . C G 141.37 . AC=8;AF=0.118;AN=68;BaseQRankSum=-0.596;DP=90;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3854;InbreedingCoeff=0.1736;MQ=56.04;MQ0=0;MQRankSum=-0.664;QD=11.78;ReadPosRankSum=0.289;EFF=DOWNSTREAM(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|),DOWNSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),DOWNSTREAM(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|),DOWNSTREAM(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|),INTERGENIC(MODIFIER|||||||||);CSQ=downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 +chr1 1138913 . T C 4314.24 . AC=14;AF=0.1167;AN=120;BaseQRankSum=2.277;DP=804;Dels=0.00;FS=7.205;HRun=4;HaplotypeScore=0.3748;InbreedingCoeff=0.2363;MQ=56.65;MQ0=0;MQRankSum=1.461;QD=20.64;ReadPosRankSum=0.717;EFF=DOWNSTREAM(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|),DOWNSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),UTR_3_PRIME(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|5),UTR_3_PRIME(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|4);CSQ=downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,3_prime_UTR_variant|||ENSG00000186891|TNFRSF18|ENST00000379268|5/5|||-/241|protein_coding|1,downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,3_prime_UTR_variant|||ENSG00000186891|TNFRSF18|ENST00000328596|4/4|||-/255|protein_coding|1 +chr1 1138963 . C T 602.78 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.829;DP=1466;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.7567;InbreedingCoeff=-0.0248;MQ=57.17;MQ0=0;MQRankSum=0.908;QD=8.74;ReadPosRankSum=0.934;EFF=DOWNSTREAM(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|),DOWNSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),UTR_3_PRIME(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|5),UTR_3_PRIME(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|4);CSQ=downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,3_prime_UTR_variant|||ENSG00000186891|TNFRSF18|ENST00000379268|5/5|||-/241|protein_coding|1,downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,3_prime_UTR_variant|||ENSG00000186891|TNFRSF18|ENST00000328596|4/4|||-/255|protein_coding|1 +chr1 1139202 . T C 2450.32 . AC=16;AF=0.1333;AN=120;BaseQRankSum=2.131;DP=874;Dels=0.00;FS=3.615;HRun=0;HaplotypeScore=0.9934;InbreedingCoeff=0.3376;MQ=57.90;MQ0=0;MQRankSum=0.238;QD=14.94;ReadPosRankSum=0.958;EFF=DOWNSTREAM(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|),DOWNSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),SYNONYMOUS_CODING(LOW|SILENT|ccA/ccG|P179|255|TNFRSF18|protein_coding|CODING|ENST00000328596|),UTR_3_PRIME(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|5);CSQ=downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,3_prime_UTR_variant|||ENSG00000186891|TNFRSF18|ENST00000379268|5/5|||-/241|protein_coding|1,downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,synonymous_variant|ccA/ccG|P|ENSG00000186891|TNFRSF18|ENST00000328596|4/4|||179/255|protein_coding|1 +chr1 1139216 . G A 192.87 . AC=3;AF=0.0250;AN=120;BaseQRankSum=1.202;DP=865;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6000;InbreedingCoeff=0.3637;MQ=58.10;MQ0=0;MQRankSum=0.774;QD=11.35;ReadPosRankSum=-0.636;EFF=DOWNSTREAM(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|),DOWNSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgt/Tgt|R175C|255|TNFRSF18|protein_coding|CODING|ENST00000328596|),UTR_3_PRIME(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|5);CSQ=downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,3_prime_UTR_variant|||ENSG00000186891|TNFRSF18|ENST00000379268|5/5|||-/241|protein_coding|1,downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,missense_variant|Cgt/Tgt|R/C|ENSG00000186891|TNFRSF18|ENST00000328596|4/4|benign(0)|tolerated(0.09)|175/255|protein_coding|1 +chr1 1139498 . C T 443.47 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-0.002;DP=812;Dels=0.00;FS=10.375;HRun=0;HaplotypeScore=0.5381;InbreedingCoeff=-0.0387;MQ=58.91;MQ0=0;MQRankSum=0.153;QD=7.92;ReadPosRankSum=-1.689;EFF=INTRON(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|3),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V101M|169|TNFRSF18|protein_coding|CODING|ENST00000486728|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V173M|234|TNFRSF18|protein_coding|CODING|ENST00000379265|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V173M|241|TNFRSF18|protein_coding|CODING|ENST00000379268|);CSQ=missense_variant|Gtg/Atg|V/M|ENSG00000186891|TNFRSF18|ENST00000379265|4/5|benign(0.2)|tolerated(0.25)|173/234|protein_coding|1,missense_variant|Gtg/Atg|V/M|ENSG00000186891|TNFRSF18|ENST00000379268|4/5|benign(0.2)|tolerated(0.26)|173/241|protein_coding|1,missense_variant|Gtg/Atg|V/M|ENSG00000186891|TNFRSF18|ENST00000486728|3/4|benign(0.2)|tolerated(0.14)|101/169|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 +chr1 1139862 . T C 4003.11 . AC=5;AF=0.0417;AN=120;BaseQRankSum=2.163;DP=3444;Dels=0.00;FS=24.530;HRun=0;HaplotypeScore=1.7331;InbreedingCoeff=0.3739;MQ=58.49;MQ0=0;MQRankSum=0.567;QD=18.88;ReadPosRankSum=-5.116;EFF=SYNONYMOUS_CODING(LOW|SILENT|aaA/aaG|K105|234|TNFRSF18|protein_coding|CODING|ENST00000379265|),SYNONYMOUS_CODING(LOW|SILENT|aaA/aaG|K105|241|TNFRSF18|protein_coding|CODING|ENST00000379268|),SYNONYMOUS_CODING(LOW|SILENT|aaA/aaG|K105|255|TNFRSF18|protein_coding|CODING|ENST00000328596|),SYNONYMOUS_CODING(LOW|SILENT|aaA/aaG|K33|169|TNFRSF18|protein_coding|CODING|ENST00000486728|);CSQ=synonymous_variant|aaA/aaG|K|ENSG00000186891|TNFRSF18|ENST00000379265|3/5|||105/234|protein_coding|1,synonymous_variant|aaA/aaG|K|ENSG00000186891|TNFRSF18|ENST00000379268|3/5|||105/241|protein_coding|1,synonymous_variant|aaA/aaG|K|ENSG00000186891|TNFRSF18|ENST00000486728|2/4|||33/169|protein_coding|1,synonymous_variant|aaA/aaG|K|ENSG00000186891|TNFRSF18|ENST00000328596|3/4|||105/255|protein_coding|1 +chr1 1140290 . T C 112.5 . AC=7;AF=0.146;AN=48;BaseQRankSum=1.165;DP=43;Dels=0.00;FS=8.424;HRun=1;HaplotypeScore=0.0347;InbreedingCoeff=0.1666;MQ=58.92;MQ0=0;MQRankSum=-0.621;QD=16.07;ReadPosRankSum=1.709;EFF=INTRON(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|1),INTRON(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|2),INTRON(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|2),INTRON(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|2);CSQ=intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 +chr1 1140336 . C A 41.02 . AC=7;AF=0.175;AN=40;BaseQRankSum=-2.498;DP=35;Dels=0.00;FS=16.166;HRun=3;HaplotypeScore=0.0909;InbreedingCoeff=0.1580;MQ=57.31;MQ0=0;MQRankSum=-0.221;QD=6.84;ReadPosRankSum=-1.676;EFF=INTRON(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|1),INTRON(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|2),INTRON(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|2),INTRON(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|2);CSQ=intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 +chr1 1140435 . G T 467.03 . AC=25;AF=0.463;AN=54;BaseQRankSum=-0.385;DP=39;Dels=0.00;FS=1.380;HRun=1;HaplotypeScore=0.0000;InbreedingCoeff=0.2592;MQ=60.00;MQ0=0;MQRankSum=0.014;QD=24.58;ReadPosRankSum=-1.128;EFF=INTRON(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|1),INTRON(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|2),INTRON(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|2),INTRON(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|2);CSQ=intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 +chr1 1140504 . T C 76.53 . AC=7;AF=0.090;AN=78;BaseQRankSum=-0.513;DP=85;Dels=0.00;FS=7.579;HRun=4;HaplotypeScore=0.1294;InbreedingCoeff=0.1345;MQ=58.35;MQ0=0;MQRankSum=0.232;QD=9.57;ReadPosRankSum=0.795;EFF=INTRON(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|1),INTRON(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|2),INTRON(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|2),INTRON(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|2);CSQ=intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 +chr1 1140812 . TC T 426.77 . AC=1;AF=0.0083;AN=120;BaseQRankSum=3.763;DP=2438;FS=2.359;HRun=1;HaplotypeScore=140.7554;InbreedingCoeff=-0.0094;MQ=58.75;MQ0=0;MQRankSum=1.559;QD=21.34;ReadPosRankSum=0.995;EFF=FRAME_SHIFT(HIGH||-|-11|169|TNFRSF18|protein_coding|CODING|ENST00000486728|1),FRAME_SHIFT(HIGH||-|-83|234|TNFRSF18|protein_coding|CODING|ENST00000379265|2),FRAME_SHIFT(HIGH||-|-83|241|TNFRSF18|protein_coding|CODING|ENST00000379268|2),FRAME_SHIFT(HIGH||-|-83|255|TNFRSF18|protein_coding|CODING|ENST00000328596|2);CSQ=frameshift_variant&feature_truncation|||ENSG00000186891|TNFRSF18|ENST00000379265|2/5|||83/234|protein_coding|1,frameshift_variant&feature_truncation|||ENSG00000186891|TNFRSF18|ENST00000379268|2/5|||83/241|protein_coding|1,frameshift_variant&feature_truncation|||ENSG00000186891|TNFRSF18|ENST00000486728|1/4|||11/169|protein_coding|1,frameshift_variant&feature_truncation|||ENSG00000186891|TNFRSF18|ENST00000328596|2/4|||83/255|protein_coding|1 +chr1 1141556 . G C 130.07 . AC=7;AF=0.097;AN=72;BaseQRankSum=0.191;DP=66;Dels=0.00;FS=4.782;HRun=3;HaplotypeScore=0.1198;InbreedingCoeff=-0.0389;MQ=57.15;MQ0=0;MQRankSum=-0.406;QD=11.82;ReadPosRankSum=-0.358;EFF=INTRON(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|1),INTRON(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|1),INTRON(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|1),UPSTREAM(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|);CSQ=intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 +chr1 1141723 . A G 877.55 . AC=13;AF=0.1204;AN=108;BaseQRankSum=0.547;DP=397;Dels=0.00;FS=2.262;HRun=3;HaplotypeScore=0.1820;InbreedingCoeff=0.0234;MQ=58.98;MQ0=0;MQRankSum=0.061;QD=11.40;ReadPosRankSum=-1.301;EFF=DOWNSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),DOWNSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),INTRON(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|1),INTRON(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|1),INTRON(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|1),UPSTREAM(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|);CSQ=intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 +chr1 1142399 . A AC 117.53 . AC=8;AF=0.308;AN=26;BaseQRankSum=-2.601;DP=22;FS=0.000;HRun=4;HaplotypeScore=4.5003;InbreedingCoeff=0.4087;MQ=58.95;MQ0=0;MQRankSum=0.702;QD=23.51;ReadPosRankSum=0.537;EFF=DOWNSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),DOWNSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|),UPSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),UPSTREAM(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|),UPSTREAM(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|);CSQ=downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 +chr1 1146478 . T C 51.13 . AC=2;AF=0.050;AN=40;BaseQRankSum=-1.556;DP=39;Dels=0.00;FS=14.569;HRun=2;HaplotypeScore=0.1961;InbreedingCoeff=-0.2329;MQ=56.11;MQ0=0;MQRankSum=-1.373;QD=5.11;ReadPosRankSum=-1.373;EFF=DOWNSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),DOWNSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),UPSTREAM(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|),UPSTREAM(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|);CSQ=downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 +chr1 1146785 . G C 472.67 . AC=4;AF=0.0333;AN=120;BaseQRankSum=0.520;DP=773;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3659;InbreedingCoeff=-0.0419;MQ=55.65;MQ0=0;MQRankSum=2.347;QD=10.28;ReadPosRankSum=0.755;EFF=DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),EXON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|5),UPSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),UPSTREAM(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|),UPSTREAM(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|),UTR_3_PRIME(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|7);CSQ=downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|5/5||||retained_intron|1,3_prime_UTR_variant|||ENSG00000186827|TNFRSF4|ENST00000379236|7/7|||-/277|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 +chr1 1146853 . G A 316.99 . AC=4;AF=0.0339;AN=118;BaseQRankSum=-0.212;DP=1329;Dels=0.00;FS=8.508;HRun=0;HaplotypeScore=1.8407;InbreedingCoeff=-0.0495;MQ=50.41;MQ0=0;MQRankSum=0.063;QD=4.12;ReadPosRankSum=-1.639;EFF=DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),EXON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|5),UPSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),UPSTREAM(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|),UPSTREAM(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|),UTR_3_PRIME(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|7);CSQ=downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|5/5||||retained_intron|1,3_prime_UTR_variant|||ENSG00000186827|TNFRSF4|ENST00000379236|7/7|||-/277|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 +chr1 1146965 . G A 553.08 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.055;DP=3459;Dels=0.00;FS=2.776;HRun=0;HaplotypeScore=2.1270;InbreedingCoeff=-0.0086;MQ=56.15;MQ0=0;MQRankSum=0.024;QD=9.22;ReadPosRankSum=0.233;EFF=DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),EXON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|5),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A268|277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|);CSQ=downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|5/5||||retained_intron|1,synonymous_variant|gcC/gcT|A|ENSG00000186827|TNFRSF4|ENST00000379236|7/7|||268/277|protein_coding|1 +chr1 1147024 . G A 429.09 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.041;DP=2325;Dels=0.00;FS=8.212;HRun=1;HaplotypeScore=2.0094;InbreedingCoeff=-0.0086;MQ=56.92;MQ0=0;MQRankSum=-0.049;QD=10.73;ReadPosRankSum=0.293;EFF=DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),EXON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|5),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|6),UPSTREAM(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|);CSQ=downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|5/5||||retained_intron|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1 +chr1 1147243 . A C 738.87 . AC=6;AF=0.0508;AN=118;BaseQRankSum=-0.595;DP=705;Dels=0.00;FS=3.973;HRun=2;HaplotypeScore=0.6191;InbreedingCoeff=-0.0615;MQ=56.78;MQ0=0;MQRankSum=-0.060;QD=13.94;ReadPosRankSum=-0.426;EFF=DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|5),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|4);CSQ=downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1 +chr1 1147297 . G A 1091.92 . AC=6;AF=0.0508;AN=118;BaseQRankSum=-0.085;DP=1113;Dels=0.00;FS=5.569;HRun=0;HaplotypeScore=0.7306;InbreedingCoeff=-0.0597;MQ=55.37;MQ0=0;MQRankSum=0.517;QD=12.55;ReadPosRankSum=1.988;EFF=DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|5),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|4);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1 +chr1 1147337 . C T 274.47 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.893;DP=1689;Dels=0.00;FS=5.279;HRun=0;HaplotypeScore=1.6439;InbreedingCoeff=-0.0134;MQ=55.97;MQ0=0;MQRankSum=3.240;QD=11.93;ReadPosRankSum=0.328;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),EXON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|4),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V207M|277|TNFRSF4|protein_coding|CODING|ENST00000379236|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,missense_variant|Gtg/Atg|V/M|ENSG00000186827|TNFRSF4|ENST00000379236|5/7|benign(0.024)|tolerated(0.21)|207/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|4/5||||retained_intron|1 +chr1 1147422 . C T 34265.37 . AC=47;AF=0.3917;AN=120;BaseQRankSum=-3.610;DP=3511;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=2.1684;InbreedingCoeff=0.1904;MQ=57.58;MQ0=0;MQRankSum=0.337;QD=16.76;ReadPosRankSum=-0.698;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),EXON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|4),EXON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|4),SYNONYMOUS_CODING(LOW|SILENT|gaG/gaA|E178|277|TNFRSF4|protein_coding|CODING|ENST00000379236|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|4/4||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,synonymous_variant|gaG/gaA|E|ENSG00000186827|TNFRSF4|ENST00000379236|5/7|||178/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|4/5||||retained_intron|1 +chr1 1147655 . G A 774.9 . AC=2;AF=0.0172;AN=116;BaseQRankSum=-2.234;DP=863;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5174;InbreedingCoeff=-0.0388;MQ=57.75;MQ0=0;MQRankSum=2.030;QD=11.07;ReadPosRankSum=-0.644;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|4),INTRON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|3),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|3);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 +chr1 1147770 . G A 69.21 . AC=2;AF=0.0196;AN=102;BaseQRankSum=-0.351;DP=166;Dels=0.00;FS=2.592;HRun=0;HaplotypeScore=0.1050;InbreedingCoeff=-0.1133;MQ=58.60;MQ0=0;MQRankSum=0.830;QD=7.69;ReadPosRankSum=-1.788;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|4),INTRON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|3),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|3);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 +chr1 1147822 . C T 59.82 . AC=2;AF=0.023;AN=88;BaseQRankSum=-1.601;DP=102;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0561;InbreedingCoeff=0.0325;MQ=56.99;MQ0=0;MQRankSum=1.803;QD=11.96;ReadPosRankSum=-2.039;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|4),INTRON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|3),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|3);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 +chr1 1147976 . A AC 257.05 . AC=13;AF=0.1182;AN=110;BaseQRankSum=3.114;DP=421;FS=9.449;HRun=9;HaplotypeScore=46.1189;InbreedingCoeff=0.0619;MQ=53.84;MQ0=0;MQRankSum=-4.864;QD=2.79;ReadPosRankSum=-0.046;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|4),INTRON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|3),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|3);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 +chr1 1147983 . C T 450.24 . AC=6;AF=0.0545;AN=110;BaseQRankSum=-1.143;DP=492;Dels=0.01;FS=1.490;HRun=0;HaplotypeScore=2.2660;InbreedingCoeff=-0.0917;MQ=53.74;MQ0=0;MQRankSum=-5.572;QD=5.85;ReadPosRankSum=-5.673;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|4),INTRON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|3),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|3);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 +chr1 1147985 . C A 474.8 . AC=7;AF=0.0648;AN=108;BaseQRankSum=1.860;DP=505;Dels=0.01;FS=0.000;HRun=1;HaplotypeScore=2.2858;InbreedingCoeff=-0.0990;MQ=53.76;MQ0=0;MQRankSum=-5.601;QD=5.59;ReadPosRankSum=-5.790;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|4),INTRON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|3),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|3);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 +chr1 1148100 . G A 407.86 . AC=4;AF=0.0333;AN=120;BaseQRankSum=0.544;DP=1027;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7645;InbreedingCoeff=-0.0459;MQ=57.31;MQ0=0;MQRankSum=2.510;QD=7.16;ReadPosRankSum=-1.015;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|3),INTRON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|2),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|2);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 +chr1 1148354 . C G 126.37 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.471;DP=949;Dels=0.00;FS=7.261;HRun=1;HaplotypeScore=0.7234;InbreedingCoeff=-0.0178;MQ=58.63;MQ0=0;MQRankSum=0.297;QD=7.43;ReadPosRankSum=0.432;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|3),INTRON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|2),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|2);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 +chr1 1148445 . G A 646.52 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-2.708;DP=1141;Dels=0.00;FS=9.672;HRun=0;HaplotypeScore=0.7418;InbreedingCoeff=-0.0474;MQ=58.91;MQ0=0;MQRankSum=1.939;QD=10.60;ReadPosRankSum=0.841;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),EXON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|2),EXON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|2),SYNONYMOUS_CODING(LOW|SILENT|tgC/tgT|C99|277|TNFRSF4|protein_coding|CODING|ENST00000379236|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|2/4||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,synonymous_variant|tgC/tgT|C|ENSG00000186827|TNFRSF4|ENST00000379236|3/7|||99/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|2/5||||retained_intron|1 +chr1 1149222 . C G 291.64 . AC=2;AF=0.0169;AN=118;BaseQRankSum=1.786;DP=868;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7514;InbreedingCoeff=-0.0246;MQ=55.49;MQ0=0;MQRankSum=1.770;QD=10.80;ReadPosRankSum=1.215;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|1),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|1),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 +chr1 1149272 . T C 57.68 . AC=9;AF=0.0750;AN=120;BaseQRankSum=-9.463;DP=854;Dels=0.00;FS=62.532;HRun=5;HaplotypeScore=2.2956;InbreedingCoeff=-0.0979;MQ=53.68;MQ0=0;MQRankSum=-8.286;QD=0.57;ReadPosRankSum=-1.613;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|1),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|1),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 +chr1 1149316 . C G 1809.62 . AC=6;AF=0.0500;AN=120;BaseQRankSum=-2.427;DP=1206;Dels=0.00;FS=7.512;HRun=0;HaplotypeScore=1.0567;InbreedingCoeff=0.2590;MQ=55.74;MQ0=0;MQRankSum=3.245;QD=14.14;ReadPosRankSum=1.300;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|1),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|1),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 +chr1 1149480 . G A 811.03 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.272;DP=1806;Dels=0.00;FS=0.697;HRun=0;HaplotypeScore=1.2218;InbreedingCoeff=-0.0227;MQ=56.24;MQ0=0;MQRankSum=1.175;QD=12.48;ReadPosRankSum=1.574;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),EXON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgc/Tgc|R10C|277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,missense_variant|Cgc/Tgc|R/C|ENSG00000186827|TNFRSF4|ENST00000379236|1/7|unknown(0)|deleterious(0)|10/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|1/5||||retained_intron|1 +chr1 1152631 . C A 73.67 . AC=6;AF=0.333;AN=18;BaseQRankSum=1.036;DP=11;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0000;MQ=57.87;MQ0=0;MQRankSum=0.000;QD=18.42;ReadPosRankSum=0.365;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|2),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|3),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),UTR_3_PRIME(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|7),UTR_3_PRIME(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|7),UTR_3_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|7);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727|7/7|||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|3/3||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|2/2||||retained_intron|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000263741|7/7|||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000360001|7/7|||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1152794 . A G 38.74 . AC=6;AF=0.250;AN=24;BaseQRankSum=0.720;DP=37;Dels=0.00;FS=0.000;HRun=3;HaplotypeScore=0.4589;InbreedingCoeff=0.4010;MQ=52.01;MQ0=0;MQRankSum=-2.348;QD=12.91;ReadPosRankSum=-0.407;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|2),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|3),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),UTR_3_PRIME(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|7),UTR_3_PRIME(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|7),UTR_3_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|7);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727|7/7|||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|3/3||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|2/2||||retained_intron|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000263741|7/7|||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000360001|7/7|||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1152798 . A C 38.42 . AC=6;AF=0.250;AN=24;BaseQRankSum=0.147;DP=40;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.4236;InbreedingCoeff=0.4263;MQ=52.65;MQ0=0;MQRankSum=-2.740;QD=12.81;ReadPosRankSum=-1.267;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|2),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|3),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),UTR_3_PRIME(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|7),UTR_3_PRIME(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|7),UTR_3_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|7);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727|7/7|||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|3/3||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|2/2||||retained_intron|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000263741|7/7|||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000360001|7/7|||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1152800 . G C 38.25 . AC=6;AF=0.250;AN=24;BaseQRankSum=-0.172;DP=40;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1945;InbreedingCoeff=0.4359;MQ=52.65;MQ0=0;MQRankSum=-2.690;QD=12.75;ReadPosRankSum=-2.003;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|2),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|3),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),UTR_3_PRIME(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|7),UTR_3_PRIME(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|7),UTR_3_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|7);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727|7/7|||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|3/3||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|2/2||||retained_intron|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000263741|7/7|||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000360001|7/7|||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1152904 . G A 446.25 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.116;DP=2117;Dels=0.00;FS=5.998;HRun=0;HaplotypeScore=1.1651;InbreedingCoeff=-0.0085;MQ=59.10;MQ0=0;MQRankSum=0.448;QD=16.53;ReadPosRankSum=0.722;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|2),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|3),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H359|362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),UTR_3_PRIME(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|7),UTR_3_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|7);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727|7/7|||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|3/3||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|2/2||||retained_intron|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000263741|7/7|||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,synonymous_variant|caC/caT|H|ENSG00000078808|SDF4|ENST00000360001|7/7|||359/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1153009 . G A 2720.69 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.647;DP=5548;Dels=0.00;FS=10.837;HRun=0;HaplotypeScore=3.0829;InbreedingCoeff=0.9964;MQ=59.14;MQ0=0;MQRankSum=-1.581;QD=29.57;ReadPosRankSum=-0.625;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|2),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|3),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A324|362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),UTR_3_PRIME(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|7),UTR_3_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|7);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727|7/7|||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|3/3||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|2/2||||retained_intron|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000263741|7/7|||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,synonymous_variant|gcC/gcT|A|ENSG00000078808|SDF4|ENST00000360001|7/7|||324/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1153113 . G A 16021.84 . AC=14;AF=0.1167;AN=120;BaseQRankSum=1.612;DP=4750;Dels=0.00;FS=29.208;HRun=1;HaplotypeScore=3.8427;InbreedingCoeff=0.1934;MQ=58.59;MQ0=0;MQRankSum=-0.299;QD=16.67;ReadPosRankSum=2.245;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|6),INTRON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|1),INTRON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|2),SYNONYMOUS_CODING(LOW|SILENT|ctC/ctT|L328|348|SDF4|protein_coding|CODING|ENST00000263741|),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),UTR_3_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|7);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727|7/7|||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,synonymous_variant|ctC/ctT|L|ENSG00000078808|SDF4|ENST00000263741|7/7|||328/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1153658 . C T 180.9 . AC=3;AF=0.0254;AN=118;BaseQRankSum=-1.007;DP=436;Dels=0.00;FS=8.257;HRun=0;HaplotypeScore=0.1613;InbreedingCoeff=-0.0432;MQ=57.74;MQ0=0;MQRankSum=-0.296;QD=10.05;ReadPosRankSum=0.424;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|6),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|6),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|6),INTRON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|1),INTRON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|2),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1153671 . C T 201.6 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.401;DP=557;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3657;InbreedingCoeff=-0.0187;MQ=57.34;MQ0=0;MQRankSum=0.747;QD=20.16;ReadPosRankSum=1.045;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|6),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|6),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|6),INTRON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|1),INTRON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|2),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1154121 . G A 626.94 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.070;DP=2668;Dels=0.00;FS=1.726;HRun=0;HaplotypeScore=1.8437;InbreedingCoeff=-0.0088;MQ=57.56;MQ0=0;MQRankSum=2.723;QD=11.61;ReadPosRankSum=-0.999;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|1),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|5),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|5),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|5),INTRON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|1),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|1/2||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1 +chr1 1154374 . G A 229.71 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.034;DP=1145;Dels=0.00;FS=3.775;HRun=1;HaplotypeScore=0.6454;InbreedingCoeff=-0.0116;MQ=58.50;MQ0=0;MQRankSum=0.802;QD=7.92;ReadPosRankSum=0.928;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|1),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|1),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|);CSQ=missense_variant|Cgg/Tgg|R/W|ENSG00000078808|SDF4|ENST00000403997|4/5|possibly_damaging(0.833)|deleterious(0.02)|132/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|1/3||||retained_intron|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|1/2||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1 +chr1 1154539 . G A 108.45 . AC=6;AF=0.068;AN=88;BaseQRankSum=-1.095;DP=122;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0697;InbreedingCoeff=0.0875;MQ=56.71;MQ0=0;MQRankSum=-1.837;QD=10.84;ReadPosRankSum=1.197;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|1),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|1),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|1/2||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1 +chr1 1158070 . C T 64.6 . AC=4;AF=0.154;AN=26;BaseQRankSum=-0.623;DP=14;Dels=0.00;FS=2.688;HRun=0;HaplotypeScore=0.1231;InbreedingCoeff=0.1888;MQ=58.33;MQ0=0;MQRankSum=-1.090;QD=21.53;ReadPosRankSum=-1.713;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1158277 . G A 258.06 . AC=7;AF=0.0660;AN=106;BaseQRankSum=1.218;DP=155;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0943;InbreedingCoeff=0.0730;MQ=58.19;MQ0=0;MQRankSum=0.130;QD=14.34;ReadPosRankSum=0.779;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1158326 . G A 805.01 . AC=13;AF=0.1102;AN=118;BaseQRankSum=1.271;DP=279;Dels=0.00;FS=5.053;HRun=0;HaplotypeScore=0.2930;InbreedingCoeff=0.1676;MQ=57.56;MQ0=0;MQRankSum=-0.687;QD=17.13;ReadPosRankSum=-0.913;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1158357 . A G 916.43 . AC=10;AF=0.0833;AN=120;BaseQRankSum=-1.540;DP=426;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.2650;InbreedingCoeff=0.0655;MQ=57.91;MQ0=0;MQRankSum=-0.479;QD=14.10;ReadPosRankSum=1.027;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1158427 . C CTCAT 100.01 . AC=7;AF=0.0583;AN=120;BaseQRankSum=4.538;DP=1071;FS=0.000;HRun=0;HaplotypeScore=95.9447;InbreedingCoeff=-0.0617;MQ=58.35;MQ0=0;MQRankSum=-4.421;QD=0.89;ReadPosRankSum=1.472;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1158441 . GCA G 2419.37 . AC=7;AF=0.0583;AN=120;BaseQRankSum=8.159;DP=1188;FS=0.719;HRun=0;HaplotypeScore=80.9615;InbreedingCoeff=-0.0625;MQ=58.49;MQ0=0;MQRankSum=0.055;QD=13.82;ReadPosRankSum=-1.278;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1158534 . G GAC 10837.11 . AC=11;AF=0.0917;AN=120;BaseQRankSum=10.503;DP=3575;FS=3.271;HRun=0;HaplotypeScore=253.2703;InbreedingCoeff=0.0992;MQ=58.17;MQ0=0;MQRankSum=-7.407;QD=20.14;ReadPosRankSum=-0.456;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1158543 . G A 443.06 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.994;DP=3849;Dels=0.00;FS=6.053;HRun=0;HaplotypeScore=6.1083;InbreedingCoeff=-0.0084;MQ=58.27;MQ0=0;MQRankSum=0.950;QD=14.77;ReadPosRankSum=-2.545;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1158562 . AAC A 12359.87 . AC=11;AF=0.0917;AN=120;BaseQRankSum=19.423;DP=4760;FS=4.102;HRun=0;HaplotypeScore=407.7083;InbreedingCoeff=0.0992;MQ=58.43;MQ0=0;MQRankSum=-2.532;QD=17.12;ReadPosRankSum=-0.503;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1158567 . A G 234.79 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-2.278;DP=5021;Dels=0.00;FS=38.170;HRun=0;HaplotypeScore=12.5431;InbreedingCoeff=-0.0170;MQ=58.49;MQ0=0;MQRankSum=-1.361;QD=1.06;ReadPosRankSum=-7.229;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1158631 . A G 241536.85 . AC=114;AF=0.9500;AN=120;BaseQRankSum=1.928;DP=8050;DS;Dels=0.00;FS=8.602;HRun=0;HaplotypeScore=3.7991;InbreedingCoeff=-0.0526;MQ=58.98;MQ0=0;MQRankSum=-0.435;QD=30.00;ReadPosRankSum=-0.542;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),EXON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),SYNONYMOUS_CODING(LOW|SILENT|gaT/gaC|D190|202|SDF4|protein_coding|CODING|ENST00000545427|),SYNONYMOUS_CODING(LOW|SILENT|gaT/gaC|D190|348|SDF4|protein_coding|CODING|ENST00000263741|),SYNONYMOUS_CODING(LOW|SILENT|gaT/gaC|D190|362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=synonymous_variant|gaT/gaC|D|ENSG00000078808|SDF4|ENST00000403997|3/5|||125/243|protein_coding|1,synonymous_variant&NMD_transcript_variant|gaT/gaC|D|ENSG00000078808|SDF4|ENST00000465727|4/7|||190/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,synonymous_variant|gaT/gaC|D|ENSG00000078808|SDF4|ENST00000263741|4/7|||190/348|protein_coding|1,synonymous_variant|gaT/gaC|D|ENSG00000078808|SDF4|ENST00000360001|4/7|||190/362|protein_coding|1,synonymous_variant|gaT/gaC|D|ENSG00000078808|SDF4|ENST00000545427|4/5|||190/202|protein_coding|1 +chr1 1158667 . G A 386.78 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.651;DP=8431;DS;Dels=0.00;FS=0.701;HRun=0;HaplotypeScore=4.5114;InbreedingCoeff=-0.0084;MQ=59.13;MQ0=0;MQRankSum=2.552;QD=11.72;ReadPosRankSum=-0.218;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),EXON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A178|202|SDF4|protein_coding|CODING|ENST00000545427|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A178|348|SDF4|protein_coding|CODING|ENST00000263741|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A178|362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=synonymous_variant|gcC/gcT|A|ENSG00000078808|SDF4|ENST00000403997|3/5|||113/243|protein_coding|1,synonymous_variant&NMD_transcript_variant|gcC/gcT|A|ENSG00000078808|SDF4|ENST00000465727|4/7|||178/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,synonymous_variant|gcC/gcT|A|ENSG00000078808|SDF4|ENST00000263741|4/7|||178/348|protein_coding|1,synonymous_variant|gcC/gcT|A|ENSG00000078808|SDF4|ENST00000360001|4/7|||178/362|protein_coding|1,synonymous_variant|gcC/gcT|A|ENSG00000078808|SDF4|ENST00000545427|4/5|||178/202|protein_coding|1 +chr1 1158948 . A G 660.52 . AC=12;AF=0.1034;AN=116;BaseQRankSum=1.771;DP=442;Dels=0.00;FS=0.846;HRun=1;HaplotypeScore=0.2202;InbreedingCoeff=0.0086;MQ=59.04;MQ0=0;MQRankSum=1.540;QD=9.57;ReadPosRankSum=1.061;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1158973 . A T 563.68 . AC=11;AF=0.0932;AN=118;BaseQRankSum=-1.928;DP=394;Dels=0.00;FS=3.023;HRun=0;HaplotypeScore=0.2474;InbreedingCoeff=0.1727;MQ=58.52;MQ0=0;MQRankSum=0.128;QD=11.74;ReadPosRankSum=-0.053;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1158975 . A C 547.46 . AC=10;AF=0.0847;AN=118;BaseQRankSum=0.388;DP=392;Dels=0.00;FS=6.029;HRun=1;HaplotypeScore=0.2379;InbreedingCoeff=0.1826;MQ=58.58;MQ0=0;MQRankSum=-0.688;QD=12.73;ReadPosRankSum=0.857;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159020 . A G 979.85 . AC=12;AF=0.1000;AN=120;BaseQRankSum=0.104;DP=496;Dels=0.00;FS=2.803;HRun=0;HaplotypeScore=0.5310;InbreedingCoeff=0.1557;MQ=58.21;MQ0=0;MQRankSum=1.781;QD=13.80;ReadPosRankSum=-2.245;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159024 . C T 1054.21 . AC=12;AF=0.1000;AN=120;BaseQRankSum=1.335;DP=502;Dels=0.00;FS=1.571;HRun=1;HaplotypeScore=0.4565;InbreedingCoeff=0.1688;MQ=58.19;MQ0=0;MQRankSum=-0.153;QD=14.25;ReadPosRankSum=-1.099;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159062 . T C 2118.4 . AC=9;AF=0.0833;AN=108;BaseQRankSum=4.132;DP=759;Dels=0.00;FS=2.568;HRun=0;HaplotypeScore=0.5732;InbreedingCoeff=0.1030;MQ=58.19;MQ0=0;MQRankSum=0.274;QD=18.11;ReadPosRankSum=0.765;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159107 . G A 3662.49 . AC=10;AF=0.0926;AN=108;BaseQRankSum=1.981;DP=1308;Dels=0.00;FS=17.272;HRun=0;HaplotypeScore=1.1121;InbreedingCoeff=0.0871;MQ=52.60;MQ0=0;MQRankSum=2.390;QD=16.35;ReadPosRankSum=1.115;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159165 . T TG 273.77 . AC=12;AF=0.1132;AN=106;BaseQRankSum=7.494;DP=2291;FS=100.981;HRun=1;HaplotypeScore=440.9019;InbreedingCoeff=-0.1415;MQ=41.07;MQ0=0;MQRankSum=-7.908;QD=0.51;ReadPosRankSum=7.023;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159166 . G GC 3218.59 . AC=42;AF=0.3962;AN=106;BaseQRankSum=8.299;DP=2329;FS=244.281;HRun=3;HaplotypeScore=445.7226;InbreedingCoeff=-0.6799;MQ=40.93;MQ0=0;MQRankSum=-10.648;QD=1.55;ReadPosRankSum=6.277;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159170 . G C 5417.07 . AC=46;AF=0.4182;AN=110;BaseQRankSum=-10.941;DP=2442;Dels=0.01;FS=490.296;HRun=4;HaplotypeScore=12.6085;InbreedingCoeff=-0.7658;MQ=41.38;MQ0=0;MQRankSum=-18.721;QD=2.30;ReadPosRankSum=6.341;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159170 . G GC 3955.45 . AC=43;AF=0.3909;AN=110;BaseQRankSum=10.923;DP=2473;FS=560.241;HRun=4;HaplotypeScore=452.2142;InbreedingCoeff=-0.6871;MQ=41.56;MQ0=0;MQRankSum=-13.253;QD=1.73;ReadPosRankSum=9.759;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159174 . CG C 53.97 . AC=7;AF=0.0614;AN=114;BaseQRankSum=10.925;DP=2603;FS=201.126;HRun=1;HaplotypeScore=455.1303;InbreedingCoeff=-0.0824;MQ=42.56;MQ0=0;MQRankSum=-11.905;QD=0.19;ReadPosRankSum=3.289;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159175 . G C 12381.61 . AC=47;AF=0.4123;AN=114;BaseQRankSum=-13.988;DP=2603;Dels=0.01;FS=959.340;HRun=4;HaplotypeScore=13.4110;InbreedingCoeff=-0.7559;MQ=42.56;MQ0=0;MQRankSum=-22.983;QD=4.80;ReadPosRankSum=11.432;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159175 . G GC 1388.08 . AC=33;AF=0.2895;AN=114;BaseQRankSum=15.983;DP=2657;FS=479.390;HRun=4;HaplotypeScore=447.3152;InbreedingCoeff=-0.4239;MQ=42.88;MQ0=0;MQRankSum=-14.282;QD=0.74;ReadPosRankSum=11.216;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159179 . A C 7593.73 . AC=46;AF=0.3898;AN=118;BaseQRankSum=-14.894;DP=2793;Dels=0.01;FS=168.118;HRun=3;HaplotypeScore=10.8241;InbreedingCoeff=-0.6865;MQ=44.03;MQ0=0;MQRankSum=-23.118;QD=2.84;ReadPosRankSum=9.565;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159179 . A AC 430.63 . AC=15;AF=0.1293;AN=116;BaseQRankSum=17.141;DP=2801;FS=268.128;HRun=3;HaplotypeScore=446.6273;InbreedingCoeff=-0.1719;MQ=43.85;MQ0=0;MQRankSum=-15.854;QD=0.50;ReadPosRankSum=8.601;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159182 . G C 2435.95 . AC=35;AF=0.3017;AN=116;BaseQRankSum=-22.569;DP=2867;Dels=0.01;FS=332.999;HRun=4;HaplotypeScore=9.7305;InbreedingCoeff=-0.4479;MQ=44.51;MQ0=0;MQRankSum=-21.997;QD=1.18;ReadPosRankSum=7.752;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159182 . G GC 290.6 . AC=11;AF=0.0948;AN=116;BaseQRankSum=18.934;DP=2960;FS=357.719;HRun=4;HaplotypeScore=463.0417;InbreedingCoeff=-0.1293;MQ=44.74;MQ0=0;MQRankSum=-17.632;QD=0.45;ReadPosRankSum=5.626;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159186 . CA C 59.87 . AC=4;AF=0.0345;AN=116;BaseQRankSum=16.532;DP=3075;FS=138.229;HRun=1;HaplotypeScore=462.1606;InbreedingCoeff=-0.0421;MQ=45.70;MQ0=0;MQRankSum=-13.262;QD=0.31;ReadPosRankSum=-6.897;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159187 . A C 8669.24 . AC=47;AF=0.4052;AN=116;BaseQRankSum=-11.349;DP=3075;Dels=0.01;FS=50.216;HRun=4;HaplotypeScore=10.3138;InbreedingCoeff=-0.7071;MQ=45.70;MQ0=0;MQRankSum=-25.197;QD=2.88;ReadPosRankSum=-1.026;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159187 . A AC 38.33 . AC=3;AF=0.0259;AN=116;BaseQRankSum=18.228;DP=3113;FS=209.724;HRun=4;HaplotypeScore=455.4440;InbreedingCoeff=-0.0431;MQ=45.73;MQ0=0;MQRankSum=-16.454;QD=0.25;ReadPosRankSum=-6.893;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159191 . A C 4227.25 . AC=45;AF=0.3814;AN=118;BaseQRankSum=-16.791;DP=3098;Dels=0.00;FS=55.651;HRun=4;HaplotypeScore=21.4245;InbreedingCoeff=-0.6257;MQ=46.09;MQ0=0;MQRankSum=-20.550;QD=1.47;ReadPosRankSum=-6.333;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159485 . C T 1786.91 . AC=11;AF=0.0917;AN=120;BaseQRankSum=-1.460;DP=676;Dels=0.00;FS=1.200;HRun=0;HaplotypeScore=0.4563;InbreedingCoeff=0.0725;MQ=57.99;MQ0=0;MQRankSum=0.146;QD=16.39;ReadPosRankSum=0.465;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159517 . T C 12932.29 . AC=115;AF=0.9583;AN=120;BaseQRankSum=1.742;DP=373;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.2819;InbreedingCoeff=0.1828;MQ=58.44;MQ0=0;MQRankSum=1.074;QD=35.14;ReadPosRankSum=1.392;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159520 . C T 92.03 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.994;DP=360;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.2826;InbreedingCoeff=-0.0403;MQ=58.45;MQ0=0;MQRankSum=0.711;QD=13.15;ReadPosRankSum=0.622;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159540 . G A 542.38 . AC=6;AF=0.0517;AN=116;BaseQRankSum=-0.694;DP=294;Dels=0.00;FS=4.088;HRun=0;HaplotypeScore=0.1520;InbreedingCoeff=0.0954;MQ=58.66;MQ0=0;MQRankSum=1.126;QD=17.50;ReadPosRankSum=0.267;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1159729 . T G 41.48 . AC=5;AF=0.119;AN=42;BaseQRankSum=0.407;DP=35;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0000;InbreedingCoeff=0.0685;MQ=55.69;MQ0=0;MQRankSum=-0.031;QD=10.37;ReadPosRankSum=0.470;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 +chr1 1163767 . C T 814.94 . AC=5;AF=0.0446;AN=112;BaseQRankSum=-1.291;DP=646;Dels=0.00;FS=1.287;HRun=0;HaplotypeScore=0.6392;InbreedingCoeff=0.1958;MQ=57.92;MQ0=0;MQRankSum=-5.203;QD=14.05;ReadPosRankSum=2.369;EFF=INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),INTRON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 +chr1 1163773 . C T 190.62 . AC=2;AF=0.0182;AN=110;BaseQRankSum=-0.471;DP=694;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6224;InbreedingCoeff=0.4388;MQ=58.00;MQ0=0;MQRankSum=0.927;QD=23.83;ReadPosRankSum=2.443;EFF=INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),INTRON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 +chr1 1163804 . C T 3932.35 . AC=16;AF=0.1356;AN=118;BaseQRankSum=-5.937;DP=1136;Dels=0.00;FS=0.471;HRun=0;HaplotypeScore=1.9937;InbreedingCoeff=-0.0388;MQ=57.51;MQ0=0;MQRankSum=2.984;QD=13.15;ReadPosRankSum=-2.512;EFF=INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),INTRON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 +chr1 1163811 . G GGACA 6953.39 . AC=15;AF=0.1271;AN=118;BaseQRankSum=-5.858;DP=1276;FS=21.541;HRun=0;HaplotypeScore=109.0684;InbreedingCoeff=-0.0171;MQ=57.57;MQ0=0;MQRankSum=-14.498;QD=29.97;ReadPosRankSum=1.598;EFF=INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),INTRON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 +chr1 1163964 . G A 5411.83 . AC=5;AF=0.0417;AN=120;BaseQRankSum=1.794;DP=3215;Dels=0.00;FS=17.078;HRun=0;HaplotypeScore=1.6463;InbreedingCoeff=0.3739;MQ=58.93;MQ0=0;MQRankSum=1.765;QD=20.19;ReadPosRankSum=-3.159;EFF=EXON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),EXON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D70|202|SDF4|protein_coding|CODING|ENST00000545427|),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D70|348|SDF4|protein_coding|CODING|ENST00000263741|),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D70|362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=synonymous_variant|gaC/gaT|D|ENSG00000078808|SDF4|ENST00000403997|1/5|||5/243|protein_coding|1,synonymous_variant&NMD_transcript_variant|gaC/gaT|D|ENSG00000078808|SDF4|ENST00000465727|2/7|||70/254|nonsense_mediated_decay|1,synonymous_variant|gaC/gaT|D|ENSG00000078808|SDF4|ENST00000360001|2/7|||70/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|2/3||||processed_transcript|1,synonymous_variant|gaC/gaT|D|ENSG00000078808|SDF4|ENST00000545427|2/5|||70/202|protein_coding|1,synonymous_variant|gaC/gaT|D|ENSG00000078808|SDF4|ENST00000263741|2/7|||70/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 +chr1 1164031 . A G 2189.26 . AC=3;AF=0.0250;AN=120;BaseQRankSum=0.524;DP=3658;DS;Dels=0.00;FS=1.511;HRun=0;HaplotypeScore=1.4260;InbreedingCoeff=0.6581;MQ=58.98;MQ0=0;MQRankSum=-0.494;QD=25.46;ReadPosRankSum=2.486;EFF=EXON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),EXON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTa/gCa|V48A|202|SDF4|protein_coding|CODING|ENST00000545427|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTa/gCa|V48A|348|SDF4|protein_coding|CODING|ENST00000263741|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTa/gCa|V48A|362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,missense_variant&NMD_transcript_variant|gTa/gCa|V/A|ENSG00000078808|SDF4|ENST00000465727|2/7|benign(0.002)|tolerated(0.47)|48/254|nonsense_mediated_decay|1,missense_variant|gTa/gCa|V/A|ENSG00000078808|SDF4|ENST00000360001|2/7|benign(0)|tolerated(1)|48/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|2/3||||processed_transcript|1,missense_variant|gTa/gCa|V/A|ENSG00000078808|SDF4|ENST00000545427|2/5|benign(0.002)|tolerated(1)|48/202|protein_coding|1,missense_variant|gTa/gCa|V/A|ENSG00000078808|SDF4|ENST00000263741|2/7|benign(0.002)|tolerated(1)|48/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 +chr1 1164072 . C T 1571.76 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-2.716;DP=2924;DS;Dels=0.00;FS=4.033;HRun=0;HaplotypeScore=1.7879;InbreedingCoeff=0.6581;MQ=59.08;MQ0=0;MQRankSum=-0.133;QD=23.11;ReadPosRankSum=3.483;EFF=EXON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),EXON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),SYNONYMOUS_CODING(LOW|SILENT|gcG/gcA|A34|202|SDF4|protein_coding|CODING|ENST00000545427|),SYNONYMOUS_CODING(LOW|SILENT|gcG/gcA|A34|348|SDF4|protein_coding|CODING|ENST00000263741|),SYNONYMOUS_CODING(LOW|SILENT|gcG/gcA|A34|362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,synonymous_variant&NMD_transcript_variant|gcG/gcA|A|ENSG00000078808|SDF4|ENST00000465727|2/7|||34/254|nonsense_mediated_decay|1,synonymous_variant|gcG/gcA|A|ENSG00000078808|SDF4|ENST00000360001|2/7|||34/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|2/3||||processed_transcript|1,synonymous_variant|gcG/gcA|A|ENSG00000078808|SDF4|ENST00000545427|2/5|||34/202|protein_coding|1,synonymous_variant|gcG/gcA|A|ENSG00000078808|SDF4|ENST00000263741|2/7|||34/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 +chr1 1164090 . T C 1638.23 . AC=3;AF=0.0250;AN=120;BaseQRankSum=0.539;DP=2459;Dels=0.00;FS=3.268;HRun=1;HaplotypeScore=1.1248;InbreedingCoeff=0.6578;MQ=59.09;MQ0=0;MQRankSum=-1.281;QD=25.20;ReadPosRankSum=2.166;EFF=EXON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),EXON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),SYNONYMOUS_CODING(LOW|SILENT|gcA/gcG|A28|202|SDF4|protein_coding|CODING|ENST00000545427|),SYNONYMOUS_CODING(LOW|SILENT|gcA/gcG|A28|348|SDF4|protein_coding|CODING|ENST00000263741|),SYNONYMOUS_CODING(LOW|SILENT|gcA/gcG|A28|362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,synonymous_variant&NMD_transcript_variant|gcA/gcG|A|ENSG00000078808|SDF4|ENST00000465727|2/7|||28/254|nonsense_mediated_decay|1,synonymous_variant|gcA/gcG|A|ENSG00000078808|SDF4|ENST00000360001|2/7|||28/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|2/3||||processed_transcript|1,synonymous_variant|gcA/gcG|A|ENSG00000078808|SDF4|ENST00000545427|2/5|||28/202|protein_coding|1,synonymous_variant|gcA/gcG|A|ENSG00000078808|SDF4|ENST00000263741|2/7|||28/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 +chr1 1164179 . G A 833.67 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.622;DP=1063;Dels=0.00;FS=8.157;HRun=0;HaplotypeScore=0.7297;InbreedingCoeff=0.6212;MQ=58.97;MQ0=0;MQRankSum=1.079;QD=26.89;ReadPosRankSum=2.348;EFF=EXON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|),UTR_5_PRIME(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),UTR_5_PRIME(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),UTR_5_PRIME(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),UTR_5_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,5_prime_UTR_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727|2/7|||-/254|nonsense_mediated_decay|1,5_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000360001|2/7|||-/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|2/3||||processed_transcript|1,5_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000545427|2/5|||-/202|protein_coding|1,5_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000263741|2/7|||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 +chr1 1164250 . C CCA 210.26 . AC=2;AF=0.0167;AN=120;BaseQRankSum=0.248;DP=413;FS=0.000;HRun=0;HaplotypeScore=29.3469;InbreedingCoeff=0.3092;MQ=58.65;MQ0=0;MQRankSum=2.358;QD=26.28;ReadPosRankSum=0.652;EFF=EXON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|),UTR_5_PRIME(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),UTR_5_PRIME(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),UTR_5_PRIME(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),UTR_5_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,5_prime_UTR_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727|2/7|||-/254|nonsense_mediated_decay|1,5_prime_UTR_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001|2/7|||-/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000459994|2/3||||processed_transcript|1,5_prime_UTR_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427|2/5|||-/202|protein_coding|1,5_prime_UTR_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741|2/7|||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 +chr1 1164687 . G C 63.9 . AC=4;AF=0.118;AN=34;BaseQRankSum=1.043;DP=28;Dels=0.00;FS=10.726;HRun=0;HaplotypeScore=0.2350;InbreedingCoeff=0.1906;MQ=58.33;MQ0=0;MQRankSum=0.241;QD=21.30;ReadPosRankSum=-1.685;EFF=INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|1),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|1),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|1),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|1),INTRON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|1),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 +chr1 1168180 . G C 380.06 . AC=9;AF=0.105;AN=86;BaseQRankSum=0.307;DP=108;Dels=0.00;FS=6.481;HRun=3;HaplotypeScore=0.2416;InbreedingCoeff=0.2106;MQ=56.49;MQ0=0;MQRankSum=1.066;QD=21.11;ReadPosRankSum=-1.425;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gaG/gaC|E174D|329|B3GALT6|protein_coding|CODING|ENST00000379198|),UPSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),UPSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),UPSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),UPSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,missense_variant|gaG/gaC|E/D|ENSG00000176022|B3GALT6|ENST00000379198|1/1|benign(0.012)|tolerated(0.54)|174/329|protein_coding|1 +chr1 1168567 . G A 197.95 . AC=1;AF=0.0098;AN=102;BaseQRankSum=-1.549;DP=173;Dels=0.00;FS=11.564;HRun=1;HaplotypeScore=0.1898;InbreedingCoeff=-0.1063;MQ=57.97;MQ0=0;MQRankSum=0.710;QD=15.23;ReadPosRankSum=-0.901;EFF=SYNONYMOUS_CODING(LOW|SILENT|gaG/gaA|E303|329|B3GALT6|protein_coding|CODING|ENST00000379198|),UPSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),UPSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),UPSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),UPSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,synonymous_variant|gaG/gaA|E|ENSG00000176022|B3GALT6|ENST00000379198|1/1|||303/329|protein_coding|1 +chr1 1168843 . C T 395.96 . AC=2;AF=0.031;AN=64;BaseQRankSum=1.429;DP=826;Dels=0.00;FS=1.955;HRun=0;HaplotypeScore=1.3582;InbreedingCoeff=-0.0815;MQ=56.66;MQ0=0;MQRankSum=0.858;QD=9.90;ReadPosRankSum=0.063;EFF=UPSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),UPSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),UPSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),UPSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),UTR_3_PRIME(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|1);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,3_prime_UTR_variant|||ENSG00000176022|B3GALT6|ENST00000379198|1/1|||-/329|protein_coding|1 +chr1 1169094 . G C 1587.89 . AC=1;AF=0.016;AN=62;BaseQRankSum=1.089;DP=2518;Dels=0.00;FS=10.659;HRun=0;HaplotypeScore=2.4242;InbreedingCoeff=-0.0613;MQ=58.81;MQ0=0;MQRankSum=1.439;QD=15.72;ReadPosRankSum=-1.308;EFF=UPSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),UPSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),UPSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),UPSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),UTR_3_PRIME(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|1);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,3_prime_UTR_variant|||ENSG00000176022|B3GALT6|ENST00000379198|1/1|||-/329|protein_coding|1 +chr1 1177474 . A G 43.95 . AC=4;AF=0.105;AN=38;BaseQRankSum=-0.867;DP=27;Dels=0.00;FS=0.000;HRun=3;HaplotypeScore=0.0000;InbreedingCoeff=0.1376;MQ=58.27;MQ0=0;MQRankSum=0.685;QD=14.65;ReadPosRankSum=1.780;EFF=DOWNSTREAM(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),INTERGENIC(MODIFIER|||||||||);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1177630 . A G 581.54 . AC=7;AF=0.092;AN=76;BaseQRankSum=-0.178;DP=115;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1257;InbreedingCoeff=0.0962;MQ=57.55;MQ0=0;MQRankSum=0.915;QD=24.23;ReadPosRankSum=0.465;EFF=DOWNSTREAM(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),INTERGENIC(MODIFIER|||||||||);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1177773 . C G 1999.45 . AC=2;AF=0.0179;AN=112;BaseQRankSum=0.305;DP=963;Dels=0.00;FS=3.764;HRun=0;HaplotypeScore=0.6634;InbreedingCoeff=0.3008;MQ=56.96;MQ0=0;MQRankSum=1.294;QD=32.25;ReadPosRankSum=-0.562;EFF=DOWNSTREAM(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),INTERGENIC(MODIFIER|||||||||);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1177893 . C T 2555.63 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.263;DP=3336;Dels=0.00;FS=2.685;HRun=0;HaplotypeScore=1.5161;InbreedingCoeff=-0.0315;MQ=56.80;MQ0=0;MQRankSum=1.301;QD=12.84;ReadPosRankSum=-1.150;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|3),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|2),UTR_3_PRIME(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|8);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|3/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000184163|FAM132A|ENST00000330388|8/8|||-/302|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000478606|2/2||||retained_intron|1 +chr1 1177918 . CT C 3669.24 . AC=4;AF=0.0333;AN=120;BaseQRankSum=7.568;DP=3999;DS;FS=3.238;HRun=1;HaplotypeScore=287.5611;InbreedingCoeff=-0.0451;MQ=56.98;MQ0=0;MQRankSum=0.885;QD=11.87;ReadPosRankSum=-0.015;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|3),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|2),UTR_3_PRIME(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|8);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000184163|FAM132A|ENST00000462849|3/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,3_prime_UTR_variant&feature_truncation|||ENSG00000184163|FAM132A|ENST00000330388|8/8|||-/302|protein_coding|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000184163|FAM132A|ENST00000478606|2/2||||retained_intron|1 +chr1 1177919 . T C 61.88 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-3.136;DP=3999;DS;Dels=0.03;FS=1.983;HRun=6;HaplotypeScore=4.0470;InbreedingCoeff=-0.0307;MQ=56.98;MQ0=0;MQRankSum=0.755;QD=0.41;ReadPosRankSum=-5.282;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|3),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|2),UTR_3_PRIME(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|8);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|3/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000184163|FAM132A|ENST00000330388|8/8|||-/302|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000478606|2/2||||retained_intron|1 +chr1 1178047 . T C 1725.37 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.230;DP=3874;DS;Dels=0.00;FS=5.482;HRun=1;HaplotypeScore=3.6848;InbreedingCoeff=-0.0204;MQ=57.02;MQ0=0;MQRankSum=-0.683;QD=10.59;ReadPosRankSum=1.820;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|7),INTRON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|2),INTRON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|1);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1178244 . G C 483.23 . AC=2;AF=0.0167;AN=120;BaseQRankSum=1.151;DP=1605;Dels=0.00;FS=2.186;HRun=1;HaplotypeScore=1.4521;InbreedingCoeff=0.7073;MQ=59.01;MQ0=0;MQRankSum=0.273;QD=32.22;ReadPosRankSum=-0.751;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|2),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cta/Gta|L261V|302|FAM132A|protein_coding|CODING|ENST00000330388|);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|2/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,missense_variant|Cta/Gta|L/V|ENSG00000184163|FAM132A|ENST00000330388|7/8|benign(0.001)|tolerated(0.98)|261/302|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000478606|1/2||||retained_intron|1 +chr1 1178245 . C T 4066.47 . AC=15;AF=0.1250;AN=120;BaseQRankSum=-1.548;DP=1603;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.3636;InbreedingCoeff=0.1530;MQ=59.01;MQ0=0;MQRankSum=1.224;QD=13.51;ReadPosRankSum=-1.253;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|2),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|1),SYNONYMOUS_CODING(LOW|SILENT|acG/acA|T260|302|FAM132A|protein_coding|CODING|ENST00000330388|);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|2/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,synonymous_variant|acG/acA|T|ENSG00000184163|FAM132A|ENST00000330388|7/8|||260/302|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000478606|1/2||||retained_intron|1 +chr1 1178453 . G A 466.91 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-3.075;DP=1354;Dels=0.00;FS=5.034;HRun=0;HaplotypeScore=0.8964;InbreedingCoeff=0.6716;MQ=57.75;MQ0=0;MQRankSum=1.619;QD=23.35;ReadPosRankSum=0.909;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|1),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|1),SYNONYMOUS_CODING(LOW|SILENT|tgC/tgT|C240|302|FAM132A|protein_coding|CODING|ENST00000330388|);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,synonymous_variant|tgC/tgT|C|ENSG00000184163|FAM132A|ENST00000330388|6/8|||240/302|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000478606|1/2||||retained_intron|1 +chr1 1178482 . A G 2936.1 . AC=13;AF=0.1121;AN=116;BaseQRankSum=2.434;DP=1386;Dels=0.00;FS=2.627;HRun=0;HaplotypeScore=0.8846;InbreedingCoeff=0.1962;MQ=57.58;MQ0=0;MQRankSum=-0.397;QD=11.84;ReadPosRankSum=1.190;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|1),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tgt/Cgt|C231R|302|FAM132A|protein_coding|CODING|ENST00000330388|);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,missense_variant|Tgt/Cgt|C/R|ENSG00000184163|FAM132A|ENST00000330388|6/8|benign(0)|tolerated(1)|231/302|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000478606|1/2||||retained_intron|1 +chr1 1178571 . C G 79.76 . AC=15;AF=0.1364;AN=110;BaseQRankSum=-6.811;DP=485;Dels=0.00;FS=26.762;HRun=6;HaplotypeScore=2.2448;InbreedingCoeff=-0.0971;MQ=52.03;MQ0=0;MQRankSum=-8.725;QD=0.69;ReadPosRankSum=1.393;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|1),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|5),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1178576 . T G 181.67 . AC=17;AF=0.1574;AN=108;BaseQRankSum=-5.916;DP=438;Dels=0.01;FS=1.537;HRun=5;HaplotypeScore=3.1212;InbreedingCoeff=-0.0423;MQ=51.34;MQ0=0;MQRankSum=-4.551;QD=1.36;ReadPosRankSum=1.353;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|1),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|5),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1178586 . T G 169.37 . AC=15;AF=0.150;AN=100;BaseQRankSum=-5.907;DP=387;Dels=0.00;FS=26.401;HRun=4;HaplotypeScore=3.0709;InbreedingCoeff=-0.2409;MQ=49.88;MQ0=0;MQRankSum=-7.180;QD=1.44;ReadPosRankSum=1.347;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|1),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|5),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1178925 . G A 1946.86 . AC=13;AF=0.1102;AN=118;BaseQRankSum=-1.965;DP=1010;Dels=0.00;FS=8.294;HRun=0;HaplotypeScore=0.9315;InbreedingCoeff=0.1268;MQ=57.94;MQ0=0;MQRankSum=0.962;QD=13.91;ReadPosRankSum=-0.443;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|3),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCc/gTc|A180V|302|FAM132A|protein_coding|CODING|ENST00000330388|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000468365|3/3||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,missense_variant|gCc/gTc|A/V|ENSG00000184163|FAM132A|ENST00000330388|5/8|possibly_damaging(0.558)|tolerated(0.06)|180/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1178935 . G A 255.43 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.108;DP=915;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6695;InbreedingCoeff=-0.0264;MQ=58.14;MQ0=0;MQRankSum=0.476;QD=14.19;ReadPosRankSum=1.215;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|4),INTRON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|2),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1179030 . G A 765.15 . AC=11;AF=0.0982;AN=112;BaseQRankSum=-0.728;DP=324;Dels=0.00;FS=3.299;HRun=0;HaplotypeScore=0.2197;InbreedingCoeff=0.1064;MQ=58.27;MQ0=0;MQRankSum=-0.685;QD=17.39;ReadPosRankSum=0.230;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|4),INTRON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|2),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1179309 . G A 1486.6 . AC=12;AF=0.1017;AN=118;BaseQRankSum=-0.202;DP=527;Dels=0.00;FS=5.698;HRun=0;HaplotypeScore=0.2810;InbreedingCoeff=0.1431;MQ=57.09;MQ0=0;MQRankSum=0.038;QD=15.65;ReadPosRankSum=-0.459;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|4),INTRON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|2),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1179416 . A C 387.28 . AC=8;AF=0.0690;AN=116;BaseQRankSum=-8.770;DP=848;Dels=0.00;FS=181.958;HRun=3;HaplotypeScore=0.9454;InbreedingCoeff=-0.0952;MQ=53.74;MQ0=0;MQRankSum=-2.436;QD=3.25;ReadPosRankSum=-1.967;EFF=EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|2),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|2),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTg/gGg|V150G|302|FAM132A|protein_coding|CODING|ENST00000330388|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000468365|2/3||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000486627|2/2||||retained_intron|1,missense_variant|gTg/gGg|V/G|ENSG00000184163|FAM132A|ENST00000330388|4/8|possibly_damaging(0.839)|deleterious(0)|150/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1179532 . C T 490.97 . AC=2;AF=0.0167;AN=120;BaseQRankSum=1.132;DP=1835;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.0586;InbreedingCoeff=-0.0210;MQ=57.90;MQ0=0;MQRankSum=1.423;QD=10.45;ReadPosRankSum=0.071;EFF=EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|2),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|2),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|3),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000468365|2/3||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000486627|2/2||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1179747 . C T 48.72 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.106;DP=2070;Dels=0.00;FS=5.993;HRun=0;HaplotypeScore=1.5289;InbreedingCoeff=-0.0284;MQ=59.19;MQ0=0;MQRankSum=-1.469;QD=6.96;ReadPosRankSum=1.335;EFF=INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|2),INTRON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|1),INTRON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|1),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1179800 . G A 782.26 . AC=2;AF=0.0172;AN=116;BaseQRankSum=-3.138;DP=2073;Dels=0.00;FS=2.190;HRun=0;HaplotypeScore=1.3315;InbreedingCoeff=-0.0420;MQ=59.44;MQ0=0;MQRankSum=-0.175;QD=9.78;ReadPosRankSum=0.512;EFF=EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|1),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G85|302|FAM132A|protein_coding|CODING|ENST00000330388|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000486627|1/2||||retained_intron|1,synonymous_variant|ggC/ggT|G|ENSG00000184163|FAM132A|ENST00000330388|2/8|||85/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1179983 . G A 2866.16 . AC=17;AF=0.1417;AN=120;BaseQRankSum=-1.868;DP=688;Dels=0.00;FS=0.855;HRun=0;HaplotypeScore=0.3402;InbreedingCoeff=0.3638;MQ=59.13;MQ0=0;MQRankSum=0.378;QD=21.71;ReadPosRankSum=-2.062;EFF=EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|1),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|1),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000486627|1/2||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1181372 . C T 142.78 . AC=9;AF=0.205;AN=44;BaseQRankSum=0.940;DP=56;Dels=0.00;FS=10.677;HRun=0;HaplotypeScore=0.1214;InbreedingCoeff=0.3107;MQ=57.48;MQ0=0;MQRankSum=0.070;QD=20.40;ReadPosRankSum=1.361;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|1),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|);CSQ=upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,downstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1181751 . T C 107.96 . AC=6;AF=0.130;AN=46;BaseQRankSum=0.029;DP=35;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0398;InbreedingCoeff=0.0106;MQ=56.62;MQ0=0;MQRankSum=0.202;QD=15.42;ReadPosRankSum=0.375;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|1),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|);CSQ=upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,downstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 +chr1 1190061 . A G 343.05 . AC=12;AF=0.176;AN=68;BaseQRankSum=1.182;DP=113;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0935;InbreedingCoeff=0.3239;MQ=57.93;MQ0=0;MQRankSum=0.135;QD=20.18;ReadPosRankSum=-0.224;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|),DOWNSTREAM(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|),DOWNSTREAM(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|),DOWNSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),DOWNSTREAM(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|),DOWNSTREAM(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|7),UTR_3_PRIME(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|7),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|8);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000349431|7/7|||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|7/7|||-/207|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390|8/8|||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1190166 . G A 197.18 . AC=1;AF=0.017;AN=58;BaseQRankSum=1.290;DP=332;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.5654;InbreedingCoeff=-0.0510;MQ=56.96;MQ0=0;MQRankSum=0.784;QD=10.38;ReadPosRankSum=-0.832;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|),DOWNSTREAM(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|),DOWNSTREAM(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|),DOWNSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),DOWNSTREAM(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|),DOWNSTREAM(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|7),UTR_3_PRIME(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|7),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|8);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000349431|7/7|||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|7/7|||-/207|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390|8/8|||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1190206 . G A 197.94 . AC=1;AF=0.015;AN=68;BaseQRankSum=-0.934;DP=520;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7388;InbreedingCoeff=-0.0641;MQ=57.17;MQ0=0;MQRankSum=2.013;QD=15.23;ReadPosRankSum=-0.458;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|),DOWNSTREAM(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|),DOWNSTREAM(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|),DOWNSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),DOWNSTREAM(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|),DOWNSTREAM(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|7),UTR_3_PRIME(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|7),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|8);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000349431|7/7|||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|7/7|||-/207|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390|8/8|||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1190207 . C T 432.13 . AC=7;AF=0.100;AN=70;BaseQRankSum=-1.418;DP=529;Dels=0.00;FS=4.930;HRun=0;HaplotypeScore=0.6967;InbreedingCoeff=0.1975;MQ=57.18;MQ0=0;MQRankSum=0.339;QD=9.39;ReadPosRankSum=1.134;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|),DOWNSTREAM(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|),DOWNSTREAM(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|),DOWNSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),DOWNSTREAM(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|),DOWNSTREAM(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|7),UTR_3_PRIME(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|7),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|8);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000349431|7/7|||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|7/7|||-/207|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390|8/8|||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1190481 . G A 116.73 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.156;DP=714;Dels=0.00;FS=12.015;HRun=1;HaplotypeScore=0.6509;InbreedingCoeff=-0.0173;MQ=59.06;MQ0=0;MQRankSum=-0.238;QD=6.49;ReadPosRankSum=1.991;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|),DOWNSTREAM(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|),DOWNSTREAM(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|7),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|7),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|6),UTR_3_PRIME(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|6),UTR_3_PRIME(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|7),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|8),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|8);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000339385|6/6|||-/224|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036|8/8|||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000349431|7/7|||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000348298|7/7|||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|7/7|||-/207|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390|8/8|||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000400929|6/6|||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1190523 . C T 321.56 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-1.643;DP=1091;Dels=0.00;FS=3.027;HRun=0;HaplotypeScore=0.7506;InbreedingCoeff=-0.0300;MQ=58.89;MQ0=0;MQRankSum=-0.522;QD=7.31;ReadPosRankSum=-0.760;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|),DOWNSTREAM(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|),DOWNSTREAM(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|7),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|7),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|6),UTR_3_PRIME(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|6),UTR_3_PRIME(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|7),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|8),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|8);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000339385|6/6|||-/224|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036|8/8|||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000349431|7/7|||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000348298|7/7|||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|7/7|||-/207|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390|8/8|||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000400929|6/6|||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1190544 . G A 120.24 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.624;DP=1337;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7866;InbreedingCoeff=-0.0117;MQ=58.85;MQ0=0;MQRankSum=-0.513;QD=4.01;ReadPosRankSum=1.551;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|),DOWNSTREAM(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|7),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|7),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|6),UTR_3_PRIME(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|6),UTR_3_PRIME(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|7),UTR_3_PRIME(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|7),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|8),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|8);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000339385|6/6|||-/224|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036|8/8|||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000349431|7/7|||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000348298|7/7|||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|7/7|||-/207|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390|8/8|||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000400929|6/6|||-/207|protein_coding|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000360466|7/7|||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1190685 . G A 695.66 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.799;DP=3117;Dels=0.00;FS=3.835;HRun=0;HaplotypeScore=1.7262;InbreedingCoeff=-0.0084;MQ=59.01;MQ0=0;MQRankSum=1.046;QD=13.38;ReadPosRankSum=-1.664;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),EXON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|2),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H174|207|UBE2J2|protein_coding|CODING|ENST00000347370|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H174|207|UBE2J2|protein_coding|CODING|ENST00000348298|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H174|207|UBE2J2|protein_coding|CODING|ENST00000400929|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H191|224|UBE2J2|protein_coding|CODING|ENST00000339385|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H226|259|UBE2J2|protein_coding|CODING|ENST00000349431|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H226|259|UBE2J2|protein_coding|CODING|ENST00000360466|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H242|275|UBE2J2|protein_coding|CODING|ENST00000400930|),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|8),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|8),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|7);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,synonymous_variant|caC/caT|H|ENSG00000160087|UBE2J2|ENST00000339385|6/6|||191/224|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215|7/7|||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036|8/8|||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,synonymous_variant|caC/caT|H|ENSG00000160087|UBE2J2|ENST00000349431|7/7|||226/259|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000467339|2/2||||processed_transcript|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,synonymous_variant|caC/caT|H|ENSG00000160087|UBE2J2|ENST00000348298|7/7|||174/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,synonymous_variant|caC/caT|H|ENSG00000160087|UBE2J2|ENST00000347370|7/7|||174/207|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390|8/8|||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,synonymous_variant|caC/caT|H|ENSG00000160087|UBE2J2|ENST00000400930|8/8|||242/275|protein_coding|1,synonymous_variant|caC/caT|H|ENSG00000160087|UBE2J2|ENST00000400929|6/6|||174/207|protein_coding|1,synonymous_variant|caC/caT|H|ENSG00000160087|UBE2J2|ENST00000360466|7/7|||226/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1190913 . CT C 3484.23 . AC=11;AF=0.0917;AN=120;BaseQRankSum=10.114;DP=1539;FS=1.866;HRun=3;HaplotypeScore=78.4459;InbreedingCoeff=0.1110;MQ=58.86;MQ0=0;MQRankSum=0.337;QD=15.98;ReadPosRankSum=0.968;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|6),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|6),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|6),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|5),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|5),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|6),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|6),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|7),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|7),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|7),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|4),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|1),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1191290 . C T 273.07 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.037;DP=1868;Dels=0.00;FS=4.676;HRun=1;HaplotypeScore=1.1539;InbreedingCoeff=-0.0090;MQ=58.69;MQ0=0;MQRankSum=0.087;QD=11.38;ReadPosRankSum=0.846;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|6),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|6),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|6),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|5),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|5),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|6),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|6),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|7),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|7),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|7),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|4),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|1),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1191589 . G A 716.56 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.146;DP=2848;Dels=0.00;FS=11.254;HRun=0;HaplotypeScore=1.5890;InbreedingCoeff=-0.0084;MQ=59.49;MQ0=0;MQRankSum=0.568;QD=9.55;ReadPosRankSum=0.273;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),EXON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|1),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|4),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|4),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|5),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|5),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|3),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|3),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000467339|1/2||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1191651 . A G 329.19 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.637;DP=1304;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5306;InbreedingCoeff=-0.0104;MQ=59.58;MQ0=0;MQRankSum=0.314;QD=16.46;ReadPosRankSum=1.231;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),EXON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|1),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|4),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|4),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|5),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|5),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|3),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|3),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000467339|1/2||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192105 . C A 38.15 . AC=5;AF=0.081;AN=62;BaseQRankSum=0.672;DP=78;Dels=0.00;FS=4.123;HRun=11;HaplotypeScore=0.3295;InbreedingCoeff=-0.0659;MQ=39.13;MQ0=0;MQRankSum=-1.521;QD=4.77;ReadPosRankSum=-0.389;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|4),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|4),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|5),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|5),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|3),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|3),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192268 . G A 34.31 . AC=9;AF=0.0763;AN=118;BaseQRankSum=-2.951;DP=712;Dels=0.00;FS=86.476;HRun=23;HaplotypeScore=4.3915;InbreedingCoeff=-0.1175;MQ=40.22;MQ0=0;MQRankSum=-4.056;QD=0.35;ReadPosRankSum=-6.599;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|4),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|4),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|5),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|5),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|3),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|3),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192321 . C A 197.5 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.375;DP=1985;Dels=0.00;FS=2.097;HRun=0;HaplotypeScore=2.7167;InbreedingCoeff=-0.0116;MQ=52.62;MQ0=0;MQRankSum=0.704;QD=10.97;ReadPosRankSum=-0.366;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|4),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|4),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|5),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|5),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|3),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|3),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192515 . A G 9144.94 . AC=10;AF=0.0833;AN=120;BaseQRankSum=-2.796;DP=4520;Dels=0.00;FS=4.056;HRun=0;HaplotypeScore=2.8736;InbreedingCoeff=0.1273;MQ=57.88;MQ0=0;MQRankSum=7.716;QD=16.19;ReadPosRankSum=-1.141;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|4),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|4),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|4),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|3),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|4),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|4),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|2),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|2),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192767 . C G 279.19 . AC=5;AF=0.0424;AN=118;BaseQRankSum=-0.503;DP=1028;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=10.9757;InbreedingCoeff=-0.0595;MQ=43.48;MQ0=0;MQRankSum=0.689;QD=3.49;ReadPosRankSum=-0.850;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192769 . CG C 46.23 . AC=14;AF=0.1167;AN=120;BaseQRankSum=7.490;DP=929;FS=8.084;HRun=1;HaplotypeScore=182.4593;InbreedingCoeff=-0.1268;MQ=41.55;MQ0=0;MQRankSum=-2.659;QD=0.18;ReadPosRankSum=0.739;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192770 . GCCA G 709.55 . AC=36;AF=0.3051;AN=118;BaseQRankSum=7.682;DP=910;FS=3.236;HRun=0;HaplotypeScore=185.0135;InbreedingCoeff=-0.3195;MQ=40.92;MQ0=0;MQRankSum=-5.310;QD=1.24;ReadPosRankSum=5.795;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192771 . CCA C 1171.87 . AC=44;AF=0.3729;AN=118;BaseQRankSum=10.317;DP=897;FS=2.917;HRun=0;HaplotypeScore=180.9024;InbreedingCoeff=-0.3867;MQ=40.58;MQ0=0;MQRankSum=-6.072;QD=1.66;ReadPosRankSum=5.982;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192772 . CA C 1504.33 . AC=45;AF=0.3879;AN=116;BaseQRankSum=10.581;DP=873;FS=9.989;HRun=1;HaplotypeScore=179.8239;InbreedingCoeff=-0.4727;MQ=39.95;MQ0=0;MQRankSum=-6.732;QD=2.06;ReadPosRankSum=6.533;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192773 . AC A 268.82 . AC=52;AF=0.4815;AN=108;BaseQRankSum=1.061;DP=867;FS=7.111;HRun=15;HaplotypeScore=182.8712;InbreedingCoeff=0.1639;MQ=39.70;MQ0=0;MQRankSum=-1.275;QD=0.46;ReadPosRankSum=4.858;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192776 . C G 174.83 . AC=3;AF=0.0273;AN=110;BaseQRankSum=-0.288;DP=833;Dels=0.01;FS=0.000;HRun=0;HaplotypeScore=8.8403;InbreedingCoeff=-0.0771;MQ=38.73;MQ0=0;MQRankSum=2.194;QD=3.64;ReadPosRankSum=-1.689;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192786 . C A 471.53 . AC=12;AF=0.1154;AN=104;BaseQRankSum=-6.703;DP=722;Dels=0.00;FS=2.215;HRun=0;HaplotypeScore=1.5194;InbreedingCoeff=0.1443;MQ=35.94;MQ0=0;MQRankSum=0.095;QD=3.27;ReadPosRankSum=2.115;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192786 . C CA 288.17 . AC=16;AF=0.1455;AN=110;BaseQRankSum=0.649;DP=716;FS=2.212;HRun=0;HaplotypeScore=181.3815;InbreedingCoeff=0.2496;MQ=36.10;MQ0=0;MQRankSum=-1.597;QD=1.84;ReadPosRankSum=-0.511;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192787 . C G 453.3 . AC=12;AF=0.120;AN=100;BaseQRankSum=2.173;DP=716;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.4128;InbreedingCoeff=0.1641;MQ=36.10;MQ0=0;MQRankSum=-0.721;QD=4.08;ReadPosRankSum=0.454;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192787 . C CG 374.1 . AC=17;AF=0.1545;AN=110;BaseQRankSum=0.800;DP=712;FS=0.000;HRun=0;HaplotypeScore=181.2272;InbreedingCoeff=0.2381;MQ=36.30;MQ0=0;MQRankSum=-3.098;QD=2.65;ReadPosRankSum=-1.333;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192789 . T C 74.78 . AC=1;AF=0.0098;AN=102;BaseQRankSum=-1.047;DP=679;Dels=0.00;FS=0.000;HRun=15;HaplotypeScore=1.3798;InbreedingCoeff=-0.0743;MQ=36.78;MQ0=0;MQRankSum=-1.425;QD=9.35;ReadPosRankSum=1.189;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1192796 . G A 212.87 . AC=4;AF=0.0364;AN=110;BaseQRankSum=-1.973;DP=615;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.2937;InbreedingCoeff=-0.0566;MQ=38.68;MQ0=0;MQRankSum=-0.959;QD=4.09;ReadPosRankSum=-2.635;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1198510 . G A 538.65 . AC=2;AF=0.0179;AN=112;BaseQRankSum=2.023;DP=404;Dels=0.00;FS=3.013;HRun=0;HaplotypeScore=0.4208;InbreedingCoeff=-0.0639;MQ=57.33;MQ0=0;MQRankSum=1.298;QD=17.96;ReadPosRankSum=1.405;EFF=DOWNSTREAM(MODIFIER||||42|UBE2J2|protein_coding|CODING|ENST00000488418|),EXON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|3),INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|3/3||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000488418||||-/43|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1198578 . C T 938.59 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-0.880;DP=1202;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.1589;InbreedingCoeff=0.4530;MQ=59.30;MQ0=0;MQRankSum=0.985;QD=21.33;ReadPosRankSum=-2.169;EFF=DOWNSTREAM(MODIFIER||||42|UBE2J2|protein_coding|CODING|ENST00000488418|),EXON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|3),INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|3/3||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000488418||||-/43|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1198618 . T A 9157.74 . AC=14;AF=0.1167;AN=120;BaseQRankSum=0.445;DP=2253;Dels=0.00;FS=1.666;HRun=1;HaplotypeScore=1.0999;InbreedingCoeff=0.1893;MQ=59.34;MQ0=0;MQRankSum=0.163;QD=16.59;ReadPosRankSum=2.122;EFF=DOWNSTREAM(MODIFIER||||42|UBE2J2|protein_coding|CODING|ENST00000488418|),EXON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|3),INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|3/3||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000488418||||-/43|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1203116 . G A 525.11 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.823;DP=1403;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.4562;InbreedingCoeff=-0.0106;MQ=58.08;MQ0=0;MQRankSum=2.079;QD=11.93;ReadPosRankSum=2.071;EFF=DOWNSTREAM(MODIFIER||||42|UBE2J2|protein_coding|CODING|ENST00000488418|),INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|2),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|2),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|2),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|1),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|2),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|2),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|1),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|2),UPSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),UPSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|2),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|2),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|2),UTR_5_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|2);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|||||retained_intron|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215|2/7|||-/44|nonsense_mediated_decay|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294|2/6|||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000488418||||-/43|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,5_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|2/7|||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752|2/6|||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1203167 . C T 1087.43 . AC=2;AF=0.0167;AN=120;BaseQRankSum=1.333;DP=2258;Dels=0.00;FS=8.468;HRun=1;HaplotypeScore=1.5625;InbreedingCoeff=0.8365;MQ=57.71;MQ0=0;MQRankSum=-0.200;QD=28.62;ReadPosRankSum=-1.052;EFF=DOWNSTREAM(MODIFIER||||42|UBE2J2|protein_coding|CODING|ENST00000488418|),INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|2),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|2),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|2),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|1),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|2),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|2),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|1),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|2),UPSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),UPSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|2),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|2),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|2),UTR_5_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|2);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|||||retained_intron|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215|2/7|||-/44|nonsense_mediated_decay|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294|2/6|||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000488418||||-/43|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,5_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|2/7|||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752|2/6|||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1203271 . G A 523.79 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.124;DP=5255;Dels=0.00;FS=0.700;HRun=0;HaplotypeScore=3.4063;InbreedingCoeff=-0.0084;MQ=58.09;MQ0=0;MQRankSum=0.171;QD=10.48;ReadPosRankSum=-1.486;EFF=EXON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|2),EXON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|2),EXON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|2),EXON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|2),EXON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|2),EXON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|2),EXON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|2),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|1),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A34|100|UBE2J2|protein_coding|CODING|ENST00000422076|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A34|195|UBE2J2|protein_coding|CODING|ENST00000435198|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A34|259|UBE2J2|protein_coding|CODING|ENST00000349431|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A34|259|UBE2J2|protein_coding|CODING|ENST00000360466|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A34|275|UBE2J2|protein_coding|CODING|ENST00000400930|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A34|42|UBE2J2|protein_coding|CODING|ENST00000488418|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A34|70|UBE2J2|protein_coding|CODING|ENST00000502382|),UPSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),UPSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UTR_5_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|2),UTR_5_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|2);CSQ=synonymous_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000502382|3/5|||34/71|protein_coding|1,synonymous_variant&NMD_transcript_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000477894|2/6|||34/100|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|2/3||||retained_intron|1,synonymous_variant&NMD_transcript_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000473215|2/7|||34/44|nonsense_mediated_decay|1,synonymous_variant&NMD_transcript_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000503294|2/6|||34/44|nonsense_mediated_decay|1,synonymous_variant&NMD_transcript_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000464036|2/8|||34/105|nonsense_mediated_decay|1,synonymous_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000488418|2/2|||34/43|protein_coding|1,synonymous_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000422076|2/5|||34/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,synonymous_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000349431|2/7|||34/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,5_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000348298|2/7|||-/207|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,5_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|2/7|||-/207|protein_coding|1,synonymous_variant&NMD_transcript_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000450390|2/8|||34/43|nonsense_mediated_decay|1,synonymous_variant&NMD_transcript_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000466752|2/6|||34/44|nonsense_mediated_decay|1,synonymous_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000400930|2/8|||34/275|protein_coding|1,synonymous_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000360466|2/7|||34/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,synonymous_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000435198|2/7|||34/196|protein_coding|1 +chr1 1203387 . G A 3095.18 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-1.839;DP=3379;Dels=0.00;FS=3.622;HRun=0;HaplotypeScore=1.5906;InbreedingCoeff=0.4826;MQ=57.66;MQ0=0;MQRankSum=0.825;QD=17.10;ReadPosRankSum=1.782;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|1),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|1),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|1),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|1),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|1),INTRON(MODIFIER||||42|UBE2J2|protein_coding|CODING|ENST00000488418|1),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|1),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|1),UPSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),UPSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000488418||||-/43|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1203464 . G A 885.88 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-4.027;DP=1394;Dels=0.00;FS=13.230;HRun=0;HaplotypeScore=0.4057;InbreedingCoeff=-0.0494;MQ=51.99;MQ0=0;MQRankSum=3.105;QD=10.42;ReadPosRankSum=-0.558;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|1),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|1),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|1),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|1),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|1),INTRON(MODIFIER||||42|UBE2J2|protein_coding|CODING|ENST00000488418|1),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|1),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|1),UPSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),UPSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000488418||||-/43|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1203573 . C T 46.79 . AC=2;AF=0.0169;AN=118;BaseQRankSum=-2.029;DP=398;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3328;InbreedingCoeff=-0.0477;MQ=57.94;MQ0=0;MQRankSum=0.064;QD=3.90;ReadPosRankSum=-2.020;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|1),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|1),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|1),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|1),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|1),INTRON(MODIFIER||||42|UBE2J2|protein_coding|CODING|ENST00000488418|1),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|1),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|1),UPSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),UPSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000488418||||-/43|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 +chr1 1219382 . C G 517.5 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-1.346;DP=1303;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5737;InbreedingCoeff=-0.0262;MQ=56.33;MQ0=0;MQRankSum=-0.677;QD=16.69;ReadPosRankSum=0.201;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),EXON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),EXON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cag/Gag|Q126E|802|SCNN1D|protein_coding|CODING|ENST00000379116|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cag/Gag|Q28E|704|SCNN1D|protein_coding|CODING|ENST00000325425|),START_GAINED(LOW||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),STOP_GAINED(HIGH|NONSENSE|tCa/tGa|S29*|138|SCNN1D|protein_coding|CODING|ENST00000470022|),UTR_5_PRIME(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),UTR_5_PRIME(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6);CSQ=5_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000338555|3/15|||-/638|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000467651|3/4||||processed_transcript|1,missense_variant&NMD_transcript_variant|Cag/Gag|Q/E|ENSG00000162572|SCNN1D|ENST00000379101|5/17|benign(0.028)||126/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,missense_variant|Cag/Gag|Q/E|ENSG00000162572|SCNN1D|ENST00000325425|2/15|benign(0.007)|deleterious(0)|28/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,stop_gained|tCa/tGa|S/*|ENSG00000162572|SCNN1D|ENST00000470022|3/4|||29/139|protein_coding|1,5_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000400928|2/14|||-/638|protein_coding|1,missense_variant|Cag/Gag|Q/E|ENSG00000162572|SCNN1D|ENST00000379116|5/18|benign(0.007)|deleterious(0)|126/802|protein_coding|1 +chr1 1219477 . T G 61.09 . AC=10;AF=0.0962;AN=104;BaseQRankSum=-2.023;DP=228;Dels=0.02;FS=0.000;HRun=9;HaplotypeScore=1.3848;InbreedingCoeff=0.0086;MQ=48.26;MQ0=0;MQRankSum=-5.204;QD=1.61;ReadPosRankSum=5.116;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1219487 . T G 252.82 . AC=16;AF=0.178;AN=90;BaseQRankSum=-6.004;DP=169;Dels=0.04;FS=31.669;HRun=9;HaplotypeScore=0.8999;InbreedingCoeff=-0.0964;MQ=41.70;MQ0=0;MQRankSum=-5.947;QD=4.29;ReadPosRankSum=5.203;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1219489 . A G 510.67 . AC=22;AF=0.244;AN=90;BaseQRankSum=-4.579;DP=161;Dels=0.04;FS=3.458;HRun=6;HaplotypeScore=0.5978;InbreedingCoeff=-0.2229;MQ=40.79;MQ0=0;MQRankSum=-5.372;QD=6.01;ReadPosRankSum=7.135;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1219495 . GT G 259.6 . AC=33;AF=0.434;AN=76;BaseQRankSum=1.135;DP=118;FS=0.000;HRun=1;HaplotypeScore=47.6513;InbreedingCoeff=0.0098;MQ=32.06;MQ0=0;MQRankSum=-2.879;QD=3.05;ReadPosRankSum=1.806;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1219503 . GTT G 181.28 . AC=26;AF=0.481;AN=54;BaseQRankSum=1.078;DP=79;FS=0.000;HRun=2;HaplotypeScore=43.9645;InbreedingCoeff=0.2483;MQ=29.00;MQ0=0;MQRankSum=1.524;QD=4.90;ReadPosRankSum=-0.049;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1219508 . GTGA G 294.79 . AC=34;AF=0.586;AN=58;BaseQRankSum=0.768;DP=70;FS=4.559;HRun=0;HaplotypeScore=41.3138;InbreedingCoeff=0.0804;MQ=29.00;MQ0=0;MQRankSum=2.030;QD=5.67;ReadPosRankSum=0.867;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1219522 . GTC G 520.05 . AC=42;AF=0.750;AN=56;BaseQRankSum=-1.486;DP=62;FS=0.000;HRun=0;HaplotypeScore=40.8056;InbreedingCoeff=0.1654;MQ=29.00;MQ0=0;MQRankSum=0.412;QD=10.61;ReadPosRankSum=2.095;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1219534 . GTT G 272.36 . AC=31;AF=0.534;AN=58;BaseQRankSum=0.856;DP=61;FS=0.000;HRun=2;HaplotypeScore=38.8244;InbreedingCoeff=0.3057;MQ=29.00;MQ0=0;MQRankSum=1.124;QD=6.64;ReadPosRankSum=0.386;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1219556 . GTT G 904.28 . AC=38;AF=0.826;AN=46;BaseQRankSum=2.314;DP=45;FS=0.000;HRun=2;HaplotypeScore=34.7120;InbreedingCoeff=0.2326;MQ=29.00;MQ0=0;MQRankSum=-0.104;QD=23.80;ReadPosRankSum=0.656;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1219565 . GTT G 199.22 . AC=18;AF=0.450;AN=40;BaseQRankSum=1.357;DP=40;FS=3.680;HRun=2;HaplotypeScore=38.8961;InbreedingCoeff=0.2109;MQ=29.00;MQ0=0;MQRankSum=-0.222;QD=7.38;ReadPosRankSum=-1.209;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1220765 . C T 169.99 . AC=1;AF=0.011;AN=92;BaseQRankSum=0.603;DP=202;Dels=0.00;FS=9.170;HRun=0;HaplotypeScore=0.1511;InbreedingCoeff=-0.0821;MQ=58.15;MQ0=0;MQRankSum=1.246;QD=18.89;ReadPosRankSum=0.238;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1220822 . G A 282.41 . AC=1;AF=0.0096;AN=104;BaseQRankSum=-0.135;DP=459;Dels=0.00;FS=7.514;HRun=0;HaplotypeScore=0.1948;InbreedingCoeff=-0.0472;MQ=57.39;MQ0=0;MQRankSum=-0.080;QD=14.12;ReadPosRankSum=0.116;EFF=INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1220954 . G A 9088.59 . AC=17;AF=0.1574;AN=108;BaseQRankSum=-2.368;DP=1910;Dels=0.00;FS=13.820;HRun=0;HaplotypeScore=1.1187;InbreedingCoeff=0.4214;MQ=58.61;MQ0=0;MQRankSum=-0.837;QD=20.20;ReadPosRankSum=-1.798;EFF=EXON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|4),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A24T|669|SCNN1D|protein_coding|CODING|ENST00000379110|),SYNONYMOUS_CODING(LOW|SILENT|tcG/tcA|S156|802|SCNN1D|protein_coding|CODING|ENST00000379116|),SYNONYMOUS_CODING(LOW|SILENT|tcG/tcA|S58|704|SCNN1D|protein_coding|CODING|ENST00000325425|);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000467651|4/4||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,synonymous_variant|tcG/tcA|S|ENSG00000162572|SCNN1D|ENST00000325425|3/15|||58/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,synonymous_variant|tcG/tcA|S|ENSG00000162572|SCNN1D|ENST00000379116|6/18|||156/802|protein_coding|1 +chr1 1221001 . A G 1982.19 . AC=3;AF=0.0273;AN=110;BaseQRankSum=-1.350;DP=1904;Dels=0.00;FS=4.488;HRun=1;HaplotypeScore=1.2454;InbreedingCoeff=0.3709;MQ=58.84;MQ0=0;MQRankSum=-1.238;QD=25.09;ReadPosRankSum=0.484;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cAg/cGg|Q172R|802|SCNN1D|protein_coding|CODING|ENST00000379116|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cAg/cGg|Q74R|704|SCNN1D|protein_coding|CODING|ENST00000325425|),SYNONYMOUS_CODING(LOW|SILENT|gcA/gcG|A39|669|SCNN1D|protein_coding|CODING|ENST00000379110|);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,missense_variant|cAg/cGg|Q/R|ENSG00000162572|SCNN1D|ENST00000325425|3/15|benign(0.108)|deleterious(0.04)|74/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,missense_variant|cAg/cGg|Q/R|ENSG00000162572|SCNN1D|ENST00000379116|6/18|benign(0.108)|deleterious(0.01)|172/802|protein_coding|1 +chr1 1221018 . C T 150.26 . AC=1;AF=0.0088;AN=114;BaseQRankSum=1.476;DP=1708;Dels=0.00;FS=1.441;HRun=0;HaplotypeScore=0.9012;InbreedingCoeff=-0.0297;MQ=58.90;MQ0=0;MQRankSum=0.438;QD=6.83;ReadPosRankSum=2.382;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cac/Tac|H178Y|802|SCNN1D|protein_coding|CODING|ENST00000379116|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cac/Tac|H80Y|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCa/gTa|A45V|669|SCNN1D|protein_coding|CODING|ENST00000379110|);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,missense_variant|Cac/Tac|H/Y|ENSG00000162572|SCNN1D|ENST00000325425|3/15|benign(0.022)|tolerated(0.53)|80/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,missense_variant|Cac/Tac|H/Y|ENSG00000162572|SCNN1D|ENST00000379116|6/18|benign(0.022)|tolerated(0.15)|178/802|protein_coding|1 +chr1 1221054 . G T 326.69 . AC=1;AF=0.0086;AN=116;BaseQRankSum=-1.105;DP=1276;Dels=0.00;FS=1.144;HRun=1;HaplotypeScore=0.9866;InbreedingCoeff=-0.0243;MQ=58.76;MQ0=0;MQRankSum=-1.148;QD=13.07;ReadPosRankSum=0.107;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|7),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|3),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|6),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1221055 . A G 407.9 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-2.031;DP=1272;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.9531;InbreedingCoeff=-0.0269;MQ=58.74;MQ0=0;MQRankSum=-1.920;QD=14.57;ReadPosRankSum=-0.545;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|7),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|3),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|6),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1221138 . G A 2422.14 . AC=12;AF=0.1000;AN=120;BaseQRankSum=1.373;DP=714;Dels=0.00;FS=4.157;HRun=0;HaplotypeScore=0.6978;InbreedingCoeff=0.2209;MQ=58.20;MQ0=0;MQRankSum=0.824;QD=19.38;ReadPosRankSum=-0.027;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|7),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|3),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|6),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1221164 . T C 91.02 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.693;DP=688;Dels=0.00;FS=9.028;HRun=0;HaplotypeScore=0.7707;InbreedingCoeff=-0.0208;MQ=57.89;MQ0=0;MQRankSum=0.498;QD=11.38;ReadPosRankSum=-0.855;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|7),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|3),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|6),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1221276 . C T 104.85 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-1.022;DP=641;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3777;InbreedingCoeff=-0.0348;MQ=57.84;MQ0=0;MQRankSum=1.773;QD=6.55;ReadPosRankSum=0.299;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|7),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|3),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|6),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1221302 . A G 519.74 . AC=2;AF=0.0167;AN=120;BaseQRankSum=1.538;DP=606;Dels=0.00;FS=12.594;HRun=0;HaplotypeScore=0.3788;InbreedingCoeff=-0.0395;MQ=57.80;MQ0=0;MQRankSum=0.895;QD=14.85;ReadPosRankSum=-0.140;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|7),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|3),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|6),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5);CSQ=splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1221488 . CAA C 470.7 . AC=1;AF=0.0083;AN=120;BaseQRankSum=3.941;DP=1231;FS=0.000;HRun=2;HaplotypeScore=83.4816;InbreedingCoeff=-0.0140;MQ=58.74;MQ0=0;MQRankSum=-0.574;QD=21.40;ReadPosRankSum=1.386;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),EXON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|6),FRAME_SHIFT(HIGH||-|-115|669|SCNN1D|protein_coding|CODING|ENST00000379110|9),FRAME_SHIFT(HIGH||-|-120|138|SCNN1D|protein_coding|CODING|ENST00000470022|4),FRAME_SHIFT(HIGH||-|-150|704|SCNN1D|protein_coding|CODING|ENST00000325425|4),FRAME_SHIFT(HIGH||-|-248|802|SCNN1D|protein_coding|CODING|ENST00000379116|7),FRAME_SHIFT(HIGH||-|-84|638|SCNN1D|protein_coding|CODING|ENST00000338555|4),FRAME_SHIFT(HIGH||-|-84|638|SCNN1D|protein_coding|CODING|ENST00000400928|3);CSQ=frameshift_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555|4/15|||84/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,frameshift_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101|6/17|||216-217/328|nonsense_mediated_decay|1,frameshift_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425|4/15|||150/704|protein_coding|1,frameshift_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000470022|4/4|||120/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,frameshift_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928|3/14|||84/638|protein_coding|1,frameshift_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116|7/18|||248/802|protein_coding|1 +chr1 1221717 . C T 1847.63 . AC=12;AF=0.1053;AN=114;BaseQRankSum=0.132;DP=473;Dels=0.00;FS=1.107;HRun=0;HaplotypeScore=0.2958;InbreedingCoeff=0.1362;MQ=58.52;MQ0=0;MQRankSum=-0.162;QD=17.77;ReadPosRankSum=1.073;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|4),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|3),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|9),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|4),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|7),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|6);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1221728 . G A 60.11 . AC=1;AF=0.0089;AN=112;BaseQRankSum=-0.547;DP=393;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.3050;InbreedingCoeff=-0.0498;MQ=58.52;MQ0=0;MQRankSum=0.131;QD=15.03;ReadPosRankSum=0.389;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|4),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|3),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|9),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|4),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|7),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|6);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1222051 . A G 730.01 . AC=3;AF=0.0259;AN=116;BaseQRankSum=-1.656;DP=566;Dels=0.00;FS=1.481;HRun=4;HaplotypeScore=0.4452;InbreedingCoeff=0.4132;MQ=57.13;MQ0=0;MQRankSum=0.440;QD=29.20;ReadPosRankSum=-0.248;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|4),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|3),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|9),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|4),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|7),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|6);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1222267 . G C 6009.85 . AC=12;AF=0.1000;AN=120;BaseQRankSum=-0.365;DP=2056;Dels=0.00;FS=2.228;HRun=5;HaplotypeScore=2.3088;InbreedingCoeff=0.2341;MQ=56.92;MQ0=0;MQRankSum=1.028;QD=15.90;ReadPosRankSum=2.427;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),EXON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|7),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGc/cCc|R180P|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGc/cCc|R180P|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGc/cCc|R211P|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGc/cCc|R246P|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGc/cCc|R344P|802|SCNN1D|protein_coding|CODING|ENST00000379116|);CSQ=missense_variant|cGc/cCc|R/P|ENSG00000162572|SCNN1D|ENST00000338555|5/15|possibly_damaging(0.646)|tolerated(0.29)|180/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,missense_variant&NMD_transcript_variant|Gcc/Ccc|A/P|ENSG00000162572|SCNN1D|ENST00000379101|7/17|unknown(0)||313/328|nonsense_mediated_decay|1,missense_variant|cGc/cCc|R/P|ENSG00000162572|SCNN1D|ENST00000325425|5/15|possibly_damaging(0.659)|tolerated(0.22)|246/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,missense_variant|cGc/cCc|R/P|ENSG00000162572|SCNN1D|ENST00000400928|4/14|possibly_damaging(0.646)|tolerated(0.29)|180/638|protein_coding|1,missense_variant|cGc/cCc|R/P|ENSG00000162572|SCNN1D|ENST00000379116|8/18|possibly_damaging(0.659)|tolerated(0.1)|344/802|protein_coding|1 +chr1 1222407 . C T 341.16 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.879;DP=1311;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.0043;InbreedingCoeff=-0.0091;MQ=58.24;MQ0=0;MQRankSum=1.305;QD=17.96;ReadPosRankSum=0.457;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|5),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|4),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|10),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|5),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|8),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|7);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1222598 . C T 248.87 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.379;DP=1688;Dels=0.00;FS=2.854;HRun=0;HaplotypeScore=1.2495;InbreedingCoeff=-0.0104;MQ=58.05;MQ0=0;MQRankSum=0.950;QD=9.95;ReadPosRankSum=0.137;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCg/gTg|A246V|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCg/gTg|A246V|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCg/gTg|A277V|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCg/gTg|A312V|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCg/gTg|A410V|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|8);CSQ=missense_variant|gCg/gTg|A/V|ENSG00000162572|SCNN1D|ENST00000338555|6/15|benign(0.005)|tolerated(0.29)|246/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|8/17|||-/328|nonsense_mediated_decay|1,missense_variant|gCg/gTg|A/V|ENSG00000162572|SCNN1D|ENST00000325425|6/15|benign(0.003)|tolerated(0.29)|312/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,missense_variant|Cgg/Tgg|R/W|ENSG00000162572|SCNN1D|ENST00000379099|1/7|unknown(0)|tolerated(0.05)|38/308|protein_coding|1,missense_variant|gCg/gTg|A/V|ENSG00000162572|SCNN1D|ENST00000400928|5/14|benign(0.005)|tolerated(0.29)|246/638|protein_coding|1,missense_variant|gCg/gTg|A/V|ENSG00000162572|SCNN1D|ENST00000379116|9/18|benign(0.003)|tolerated(0.28)|410/802|protein_coding|1 +chr1 1222690 . C T 972.9 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-2.953;DP=1085;Dels=0.00;FS=4.145;HRun=0;HaplotypeScore=2.3729;InbreedingCoeff=-0.0488;MQ=53.44;MQ0=0;MQRankSum=5.040;QD=11.58;ReadPosRankSum=-1.089;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|6),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|5),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|11),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|6),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|9),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|8);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1222695 . G C 2613.18 . AC=15;AF=0.1250;AN=120;BaseQRankSum=-2.534;DP=1083;Dels=0.00;FS=12.740;HRun=1;HaplotypeScore=2.6047;InbreedingCoeff=0.2827;MQ=53.52;MQ0=0;MQRankSum=0.965;QD=13.75;ReadPosRankSum=2.874;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|6),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|5),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|11),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|6),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|9),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|8);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1222739 . C G 156.37 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.552;DP=1353;Dels=0.00;FS=1.709;HRun=0;HaplotypeScore=1.1586;InbreedingCoeff=-0.0117;MQ=56.35;MQ0=0;MQRankSum=-0.469;QD=9.20;ReadPosRankSum=0.855;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|6),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|5),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|11),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|6),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|9),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|8);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1222842 . C T 701.62 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-4.452;DP=3950;Dels=0.00;FS=3.995;HRun=0;HaplotypeScore=2.7986;InbreedingCoeff=-0.0085;MQ=57.76;MQ0=0;MQRankSum=2.038;QD=6.05;ReadPosRankSum=-0.162;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|6),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|5),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|11),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|6),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|9),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|8);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1222958 . C T 840.29 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.301;DP=3509;DS;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=2.2081;InbreedingCoeff=-0.0084;MQ=58.04;MQ0=0;MQRankSum=0.486;QD=11.20;ReadPosRankSum=-3.869;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgc/Tgc|R297C|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgc/Tgc|R297C|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgc/Tgc|R328C|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgc/Tgc|R363C|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgc/Tgc|R461C|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|9);CSQ=missense_variant|Cgc/Tgc|R/C|ENSG00000162572|SCNN1D|ENST00000338555|7/15|benign(0.037)|tolerated(0.18)|297/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|9/17|||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,missense_variant|Cgc/Tgc|R/C|ENSG00000162572|SCNN1D|ENST00000325425|7/15|benign(0.075)|tolerated(0.15)|363/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,synonymous_variant|agC/agT|S|ENSG00000162572|SCNN1D|ENST00000379099|2/7|||88/308|protein_coding|1,missense_variant|Cgc/Tgc|R/C|ENSG00000162572|SCNN1D|ENST00000400928|6/14|benign(0.037)|tolerated(0.18)|297/638|protein_coding|1,missense_variant|Cgc/Tgc|R/C|ENSG00000162572|SCNN1D|ENST00000379116|10/18|benign(0.075)|tolerated(0.05)|461/802|protein_coding|1 +chr1 1222963 . C T 1912.86 . AC=2;AF=0.0167;AN=120;BaseQRankSum=0.048;DP=3402;DS;Dels=0.00;FS=5.465;HRun=0;HaplotypeScore=2.6679;InbreedingCoeff=-0.0170;MQ=58.00;MQ0=0;MQRankSum=-1.133;QD=11.45;ReadPosRankSum=1.587;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P298|638|SCNN1D|protein_coding|CODING|ENST00000338555|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P298|638|SCNN1D|protein_coding|CODING|ENST00000400928|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P329|669|SCNN1D|protein_coding|CODING|ENST00000379110|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P364|704|SCNN1D|protein_coding|CODING|ENST00000325425|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P462|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|9);CSQ=synonymous_variant|ccC/ccT|P|ENSG00000162572|SCNN1D|ENST00000338555|7/15|||298/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|9/17|||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,synonymous_variant|ccC/ccT|P|ENSG00000162572|SCNN1D|ENST00000325425|7/15|||364/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,missense_variant|cCg/cTg|P/L|ENSG00000162572|SCNN1D|ENST00000379099|2/7|benign(0.04)|deleterious(0)|90/308|protein_coding|1,synonymous_variant|ccC/ccT|P|ENSG00000162572|SCNN1D|ENST00000400928|6/14|||298/638|protein_coding|1,synonymous_variant|ccC/ccT|P|ENSG00000162572|SCNN1D|ENST00000379116|10/18|||462/802|protein_coding|1 +chr1 1223385 . G C 1473.29 . AC=14;AF=0.1250;AN=112;BaseQRankSum=0.369;DP=567;Dels=0.00;FS=6.998;HRun=0;HaplotypeScore=0.6667;InbreedingCoeff=0.0907;MQ=56.62;MQ0=0;MQRankSum=0.356;QD=13.15;ReadPosRankSum=0.501;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gag/Cag|E380Q|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gag/Cag|E380Q|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gag/Cag|E411Q|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gag/Cag|E446Q|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gag/Cag|E544Q|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=missense_variant|Gag/Cag|E/Q|ENSG00000162572|SCNN1D|ENST00000338555|9/15|benign(0.006)|tolerated(0.57)|380/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|11/17|||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,missense_variant|Gag/Cag|E/Q|ENSG00000162572|SCNN1D|ENST00000325425|9/15|benign(0.012)|tolerated(0.54)|446/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,missense_variant|Gag/Cag|E/Q|ENSG00000162572|SCNN1D|ENST00000379099|3/7|benign(0.047)|tolerated(0.87)|197/308|protein_coding|1,missense_variant|Gag/Cag|E/Q|ENSG00000162572|SCNN1D|ENST00000400928|8/14|benign(0.006)|tolerated(0.57)|380/638|protein_coding|1,missense_variant|Gag/Cag|E/Q|ENSG00000162572|SCNN1D|ENST00000379116|12/18|benign(0.012)|tolerated(0.47)|544/802|protein_coding|1 +chr1 1223465 . GC G 883.64 . AC=11;AF=0.110;AN=100;BaseQRankSum=3.043;DP=292;FS=1.960;HRun=2;HaplotypeScore=26.6146;InbreedingCoeff=0.0959;MQ=54.67;MQ0=0;MQRankSum=1.559;QD=15.24;ReadPosRankSum=1.688;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223570 . C T 69.93 . AC=6;AF=0.065;AN=92;BaseQRankSum=0.235;DP=128;Dels=0.00;FS=2.433;HRun=0;HaplotypeScore=0.0933;InbreedingCoeff=0.2052;MQ=53.21;MQ0=0;MQRankSum=0.920;QD=17.48;ReadPosRankSum=-0.706;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223572 . C T 52.77 . AC=4;AF=0.044;AN=90;BaseQRankSum=1.225;DP=128;Dels=0.00;FS=2.461;HRun=0;HaplotypeScore=0.0513;InbreedingCoeff=0.1293;MQ=53.41;MQ0=0;MQRankSum=1.609;QD=17.59;ReadPosRankSum=-0.758;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223584 . T C 172.88 . AC=12;AF=0.128;AN=94;BaseQRankSum=-0.765;DP=109;Dels=0.00;FS=5.887;HRun=3;HaplotypeScore=0.0818;InbreedingCoeff=0.1717;MQ=52.94;MQ0=0;MQRankSum=-1.734;QD=15.72;ReadPosRankSum=0.356;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223608 . T C 97.92 . AC=5;AF=0.060;AN=84;BaseQRankSum=0.588;DP=98;Dels=0.00;FS=1.905;HRun=1;HaplotypeScore=0.1871;InbreedingCoeff=0.0757;MQ=49.92;MQ0=0;MQRankSum=0.349;QD=13.99;ReadPosRankSum=1.013;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223626 . T C 50.01 . AC=6;AF=0.081;AN=74;BaseQRankSum=-0.295;DP=80;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.4822;InbreedingCoeff=0.0222;MQ=49.62;MQ0=0;MQRankSum=0.318;QD=7.14;ReadPosRankSum=-0.200;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223660 . A T 124.16 . AC=11;AF=0.183;AN=60;BaseQRankSum=-2.177;DP=70;Dels=0.00;FS=6.078;HRun=0;HaplotypeScore=0.4653;InbreedingCoeff=0.0992;MQ=47.05;MQ0=0;MQRankSum=-3.819;QD=6.21;ReadPosRankSum=-0.646;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223669 . T C 30.84 . AC=2;AF=0.037;AN=54;BaseQRankSum=-0.298;DP=66;Dels=0.00;FS=2.157;HRun=2;HaplotypeScore=0.3049;InbreedingCoeff=-0.1912;MQ=46.88;MQ0=0;MQRankSum=-2.343;QD=3.85;ReadPosRankSum=-1.235;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223721 . C T 44.82 . AC=6;AF=0.150;AN=40;BaseQRankSum=0.689;DP=40;Dels=0.00;FS=2.004;HRun=0;HaplotypeScore=0.3378;InbreedingCoeff=0.0355;MQ=46.71;MQ0=0;MQRankSum=-1.020;QD=4.07;ReadPosRankSum=-1.793;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223741 . T C 53.75 . AC=4;AF=0.105;AN=38;BaseQRankSum=-0.303;DP=42;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.3143;InbreedingCoeff=-0.0438;MQ=43.53;MQ0=0;MQRankSum=1.186;QD=4.48;ReadPosRankSum=-0.745;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223754 . C T 577.41 . AC=26;AF=0.619;AN=42;BaseQRankSum=1.117;DP=42;Dels=0.00;FS=5.016;HRun=0;HaplotypeScore=0.4750;InbreedingCoeff=-0.0583;MQ=42.10;MQ0=0;MQRankSum=1.817;QD=16.04;ReadPosRankSum=-0.617;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223759 . T C 283.09 . AC=16;AF=0.400;AN=40;BaseQRankSum=0.405;DP=42;Dels=0.00;FS=20.948;HRun=2;HaplotypeScore=0.3988;InbreedingCoeff=0.0952;MQ=42.43;MQ0=0;MQRankSum=2.503;QD=12.31;ReadPosRankSum=-1.841;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223772 . C T 118.73 . AC=8;AF=0.235;AN=34;BaseQRankSum=0.790;DP=42;Dels=0.02;FS=6.468;HRun=0;HaplotypeScore=0.2464;InbreedingCoeff=-0.0704;MQ=39.82;MQ0=0;MQRankSum=-1.874;QD=6.25;ReadPosRankSum=0.384;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223790 . C T 300.88 . AC=13;AF=0.433;AN=30;BaseQRankSum=1.925;DP=47;Dels=0.04;FS=9.204;HRun=0;HaplotypeScore=0.6820;InbreedingCoeff=-0.0492;MQ=39.36;MQ0=0;MQRankSum=-0.298;QD=10.38;ReadPosRankSum=-3.275;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223795 . T C 177.98 . AC=11;AF=0.324;AN=34;BaseQRankSum=1.748;DP=49;Dels=0.00;FS=11.458;HRun=2;HaplotypeScore=1.0405;InbreedingCoeff=0.1689;MQ=39.01;MQ0=0;MQRankSum=0.452;QD=10.47;ReadPosRankSum=-2.573;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223808 . C T 254.07 . AC=14;AF=0.412;AN=34;BaseQRankSum=0.313;DP=55;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.8609;InbreedingCoeff=-0.0598;MQ=39.41;MQ0=0;MQRankSum=-2.458;QD=7.70;ReadPosRankSum=0.432;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223826 . T C 75.31 . AC=9;AF=0.188;AN=48;BaseQRankSum=-1.209;DP=64;Dels=0.00;FS=1.930;HRun=1;HaplotypeScore=0.5668;InbreedingCoeff=0.0706;MQ=39.48;MQ0=0;MQRankSum=-1.000;QD=4.71;ReadPosRankSum=0.045;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223831 . T C 93.99 . AC=8;AF=0.174;AN=46;BaseQRankSum=0.839;DP=67;Dels=0.00;FS=1.794;HRun=2;HaplotypeScore=0.6496;InbreedingCoeff=0.1389;MQ=39.56;MQ0=0;MQRankSum=-1.754;QD=7.23;ReadPosRankSum=-1.754;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223846 . T C 55.02 . AC=8;AF=0.143;AN=56;BaseQRankSum=2.634;DP=80;Dels=0.00;FS=6.864;HRun=1;HaplotypeScore=0.8057;InbreedingCoeff=-0.0215;MQ=39.58;MQ0=0;MQRankSum=-2.072;QD=2.50;ReadPosRankSum=-0.901;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223851 . T C 109.95 . AC=9;AF=0.161;AN=56;BaseQRankSum=-1.835;DP=80;Dels=0.00;FS=1.589;HRun=2;HaplotypeScore=0.5852;InbreedingCoeff=-0.0310;MQ=40.14;MQ0=0;MQRankSum=-1.699;QD=3.55;ReadPosRankSum=1.584;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223864 . T C 42.18 . AC=6;AF=0.094;AN=64;BaseQRankSum=-0.935;DP=85;Dels=0.00;FS=8.168;HRun=1;HaplotypeScore=0.4761;InbreedingCoeff=-0.1023;MQ=39.89;MQ0=0;MQRankSum=-1.619;QD=3.52;ReadPosRankSum=0.274;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223873 . T TCC 58.95 . AC=5;AF=0.076;AN=66;BaseQRankSum=2.703;DP=91;FS=1.912;HRun=3;HaplotypeScore=29.9562;InbreedingCoeff=-0.1751;MQ=40.59;MQ0=0;MQRankSum=-2.282;QD=3.27;ReadPosRankSum=-0.842;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223887 . C T 208.26 . AC=15;AF=0.242;AN=62;BaseQRankSum=1.248;DP=86;Dels=0.00;FS=1.200;HRun=0;HaplotypeScore=0.4017;InbreedingCoeff=-0.1357;MQ=40.83;MQ0=0;MQRankSum=-2.152;QD=4.84;ReadPosRankSum=0.390;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223900 . C T 905.84 . AC=36;AF=0.643;AN=56;BaseQRankSum=0.938;DP=77;Dels=0.00;FS=0.998;HRun=0;HaplotypeScore=0.6461;InbreedingCoeff=0.0959;MQ=40.99;MQ0=0;MQRankSum=-1.278;QD=14.38;ReadPosRankSum=0.258;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223905 . T C 98.29 . AC=9;AF=0.167;AN=54;BaseQRankSum=-1.946;DP=77;Dels=0.00;FS=3.277;HRun=2;HaplotypeScore=0.5934;InbreedingCoeff=0.1031;MQ=40.55;MQ0=0;MQRankSum=-0.402;QD=5.46;ReadPosRankSum=-2.098;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1223963 . T C 128.73 . AC=10;AF=0.385;AN=26;BaseQRankSum=-0.635;DP=29;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.4996;InbreedingCoeff=-0.0973;MQ=39.99;MQ0=0;MQRankSum=1.102;QD=6.78;ReadPosRankSum=0.301;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225096 . C T 123.66 . AC=9;AF=0.563;AN=16;BaseQRankSum=0.278;DP=16;Dels=0.00;FS=6.990;HRun=0;HaplotypeScore=0.8108;MQ=33.81;MQ0=0;MQRankSum=-1.246;QD=13.74;ReadPosRankSum=1.769;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225121 . T C 79.61 . AC=9;AF=0.300;AN=30;BaseQRankSum=-0.698;DP=26;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=1.1615;InbreedingCoeff=0.2471;MQ=30.87;MQ0=0;MQRankSum=-1.397;QD=11.37;ReadPosRankSum=-0.698;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225125 . TCC T 136.07 . AC=9;AF=0.281;AN=32;BaseQRankSum=0.701;DP=25;FS=18.560;HRun=5;HaplotypeScore=19.9646;InbreedingCoeff=-0.0780;MQ=30.60;MQ0=0;MQRankSum=2.186;QD=11.34;ReadPosRankSum=1.196;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225141 . T C 32.44 . AC=5;AF=0.147;AN=34;BaseQRankSum=-0.213;DP=26;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.3484;InbreedingCoeff=0.0778;MQ=30.54;MQ0=0;MQRankSum=-1.319;QD=8.11;ReadPosRankSum=-1.149;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225163 . TCC T 121.19 . AC=12;AF=0.353;AN=34;BaseQRankSum=0.452;DP=22;FS=9.260;HRun=5;HaplotypeScore=17.1552;InbreedingCoeff=0.3248;MQ=32.45;MQ0=0;MQRankSum=-1.759;QD=17.31;ReadPosRankSum=0.050;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225174 . C T 237.41 . AC=18;AF=0.643;AN=28;BaseQRankSum=0.648;DP=22;Dels=0.00;FS=2.363;HRun=0;HaplotypeScore=1.0277;InbreedingCoeff=0.3305;MQ=32.25;MQ0=0;MQRankSum=-0.648;QD=26.38;ReadPosRankSum=-0.059;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225239 . A T 100.39 . AC=6;AF=0.500;AN=12;BaseQRankSum=0.550;DP=8;Dels=0.00;FS=3.680;HRun=0;HaplotypeScore=0.0000;MQ=42.45;MQ0=0;MQRankSum=0.550;QD=25.10;ReadPosRankSum=0.550;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225261 . T C 133.06 . AC=10;AF=0.200;AN=50;BaseQRankSum=0.107;DP=45;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.3102;InbreedingCoeff=0.1724;MQ=37.78;MQ0=0;MQRankSum=-0.997;QD=14.78;ReadPosRankSum=-0.534;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225279 . T C 111.05 . AC=13;AF=0.210;AN=62;BaseQRankSum=0.114;DP=66;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.5124;InbreedingCoeff=0.2853;MQ=35.30;MQ0=0;MQRankSum=-1.427;QD=11.10;ReadPosRankSum=-1.376;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225284 . T C 256.36 . AC=13;AF=0.210;AN=62;BaseQRankSum=0.970;DP=68;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.5134;InbreedingCoeff=0.1617;MQ=35.13;MQ0=0;MQRankSum=-1.184;QD=12.82;ReadPosRankSum=0.233;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225302 . C T 193.86 . AC=15;AF=0.242;AN=62;BaseQRankSum=-1.436;DP=91;Dels=0.00;FS=1.834;HRun=0;HaplotypeScore=1.1816;InbreedingCoeff=0.2195;MQ=33.76;MQ0=0;MQRankSum=0.576;QD=8.43;ReadPosRankSum=1.178;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225312 . G A 158.46 . AC=11;AF=0.172;AN=64;BaseQRankSum=-0.966;DP=93;Dels=0.02;FS=7.390;HRun=0;HaplotypeScore=1.6923;InbreedingCoeff=-0.0065;MQ=33.88;MQ0=0;MQRankSum=-1.247;QD=6.09;ReadPosRankSum=0.910;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225319 . T C 311.15 . AC=16;AF=0.229;AN=70;BaseQRankSum=0.007;DP=97;Dels=0.00;FS=12.678;HRun=1;HaplotypeScore=0.6200;InbreedingCoeff=0.0685;MQ=35.32;MQ0=0;MQRankSum=-2.217;QD=9.15;ReadPosRankSum=2.287;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225325 . G A 205.95 . AC=12;AF=0.176;AN=68;BaseQRankSum=-2.064;DP=103;Dels=0.00;FS=4.701;HRun=0;HaplotypeScore=0.5398;InbreedingCoeff=-0.0183;MQ=36.25;MQ0=0;MQRankSum=-2.585;QD=6.86;ReadPosRankSum=1.419;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225330 . G A 109.49 . AC=8;AF=0.129;AN=62;BaseQRankSum=-2.003;DP=109;Dels=0.00;FS=4.693;HRun=0;HaplotypeScore=0.7228;InbreedingCoeff=-0.1427;MQ=36.47;MQ0=0;MQRankSum=-2.668;QD=3.78;ReadPosRankSum=0.963;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225335 . T C 238.86 . AC=13;AF=0.191;AN=68;BaseQRankSum=-1.449;DP=110;Dels=0.00;FS=7.186;HRun=1;HaplotypeScore=0.5462;InbreedingCoeff=-0.0481;MQ=36.64;MQ0=0;MQRankSum=-2.715;QD=6.46;ReadPosRankSum=-1.710;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225340 . TC T 227.19 . AC=9;AF=0.132;AN=68;BaseQRankSum=5.045;DP=112;FS=7.004;HRun=2;HaplotypeScore=30.7520;InbreedingCoeff=-0.0950;MQ=37.06;MQ0=0;MQRankSum=-2.051;QD=7.33;ReadPosRankSum=-0.410;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225343 . G A 137.99 . AC=9;AF=0.141;AN=64;BaseQRankSum=-2.584;DP=116;Dels=0.01;FS=2.292;HRun=0;HaplotypeScore=0.6542;InbreedingCoeff=-0.1498;MQ=37.81;MQ0=0;MQRankSum=-2.776;QD=4.45;ReadPosRankSum=-2.598;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225350 . G C 61.74 . AC=4;AF=0.065;AN=62;BaseQRankSum=-1.443;DP=105;Dels=0.00;FS=2.393;HRun=5;HaplotypeScore=0.7473;InbreedingCoeff=-0.1912;MQ=39.32;MQ0=0;MQRankSum=-2.240;QD=4.75;ReadPosRankSum=-3.424;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225371 . C T 52.17 . AC=8;AF=0.114;AN=70;BaseQRankSum=0.850;DP=87;Dels=0.00;FS=2.411;HRun=0;HaplotypeScore=0.1882;InbreedingCoeff=0.0721;MQ=42.92;MQ0=0;MQRankSum=-1.185;QD=3.07;ReadPosRankSum=-0.451;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225389 . C T 253.01 . AC=13;AF=0.167;AN=78;BaseQRankSum=1.797;DP=127;Dels=0.00;FS=3.410;HRun=0;HaplotypeScore=0.3513;InbreedingCoeff=0.1116;MQ=41.93;MQ0=0;MQRankSum=-1.326;QD=9.04;ReadPosRankSum=-2.083;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225407 . T C 47.15 . AC=5;AF=0.054;AN=92;BaseQRankSum=-1.004;DP=162;Dels=0.00;FS=2.276;HRun=1;HaplotypeScore=0.5729;InbreedingCoeff=-0.1734;MQ=42.85;MQ0=0;MQRankSum=-1.615;QD=1.68;ReadPosRankSum=2.014;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225425 . C T 793.83 . AC=31;AF=0.337;AN=92;BaseQRankSum=1.412;DP=187;Dels=0.02;FS=6.178;HRun=0;HaplotypeScore=0.4105;InbreedingCoeff=-0.0399;MQ=43.23;MQ0=0;MQRankSum=-4.142;QD=6.56;ReadPosRankSum=0.059;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225443 . T C 468.02 . AC=15;AF=0.163;AN=92;BaseQRankSum=-2.292;DP=235;Dels=0.00;FS=6.953;HRun=1;HaplotypeScore=0.6031;InbreedingCoeff=-0.1815;MQ=43.46;MQ0=0;MQRankSum=-0.753;QD=4.78;ReadPosRankSum=-1.745;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225452 . T TCC 330.82 . AC=10;AF=0.104;AN=96;BaseQRankSum=1.749;DP=270;FS=4.169;HRun=3;HaplotypeScore=57.3296;InbreedingCoeff=-0.1229;MQ=43.34;MQ0=0;MQRankSum=-2.041;QD=5.09;ReadPosRankSum=-2.421;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225461 . T C 238.55 . AC=14;AF=0.1273;AN=110;BaseQRankSum=-0.273;DP=319;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.9594;InbreedingCoeff=-0.1003;MQ=43.75;MQ0=0;MQRankSum=-1.815;QD=2.41;ReadPosRankSum=0.946;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225466 . T C 184.63 . AC=11;AF=0.0948;AN=116;BaseQRankSum=-1.477;DP=342;Dels=0.00;FS=1.813;HRun=2;HaplotypeScore=0.9329;InbreedingCoeff=-0.1688;MQ=44.09;MQ0=0;MQRankSum=-2.845;QD=1.85;ReadPosRankSum=-1.214;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225470 . T TCC 366.85 . AC=11;AF=0.0948;AN=116;BaseQRankSum=3.645;DP=363;FS=6.315;HRun=3;HaplotypeScore=61.2598;InbreedingCoeff=-0.1667;MQ=44.55;MQ0=0;MQRankSum=-2.973;QD=4.32;ReadPosRankSum=-1.690;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225479 . T C 216.38 . AC=8;AF=0.0690;AN=116;BaseQRankSum=-0.622;DP=392;Dels=0.00;FS=4.070;HRun=1;HaplotypeScore=1.0837;InbreedingCoeff=0.0601;MQ=45.51;MQ0=0;MQRankSum=-3.522;QD=3.73;ReadPosRankSum=-0.410;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225484 . T C 214.92 . AC=16;AF=0.1379;AN=116;BaseQRankSum=-1.446;DP=412;Dels=0.00;FS=17.524;HRun=2;HaplotypeScore=1.1210;InbreedingCoeff=-0.1039;MQ=45.67;MQ0=0;MQRankSum=-3.630;QD=1.52;ReadPosRankSum=1.200;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225488 . T TCC 73.7 . AC=6;AF=0.0517;AN=116;BaseQRankSum=3.896;DP=442;FS=2.167;HRun=3;HaplotypeScore=66.4298;InbreedingCoeff=-0.0944;MQ=46.00;MQ0=0;MQRankSum=-2.159;QD=1.23;ReadPosRankSum=-2.147;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225497 . T C 35.56 . AC=5;AF=0.0439;AN=114;BaseQRankSum=-3.665;DP=477;Dels=0.00;FS=3.756;HRun=1;HaplotypeScore=1.4250;InbreedingCoeff=-0.0704;MQ=45.97;MQ0=0;MQRankSum=-2.358;QD=0.73;ReadPosRankSum=0.893;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225502 . T C 688.93 . AC=16;AF=0.1404;AN=114;BaseQRankSum=0.243;DP=497;Dels=0.00;FS=19.856;HRun=2;HaplotypeScore=1.6402;InbreedingCoeff=-0.0754;MQ=46.34;MQ0=0;MQRankSum=-4.470;QD=5.22;ReadPosRankSum=0.940;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225504 . C T 33.71 . AC=3;AF=0.0263;AN=114;BaseQRankSum=-2.542;DP=505;Dels=0.00;FS=4.534;HRun=0;HaplotypeScore=1.6971;InbreedingCoeff=-0.0570;MQ=46.78;MQ0=0;MQRankSum=1.064;QD=1.16;ReadPosRankSum=2.168;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225505 . G A 87.65 . AC=6;AF=0.0526;AN=114;BaseQRankSum=0.061;DP=508;Dels=0.01;FS=0.000;HRun=0;HaplotypeScore=1.7900;InbreedingCoeff=-0.0837;MQ=47.10;MQ0=0;MQRankSum=-0.730;QD=1.65;ReadPosRankSum=0.134;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225506 . TCC T 120.88 . AC=8;AF=0.0702;AN=114;BaseQRankSum=7.487;DP=525;FS=3.030;HRun=5;HaplotypeScore=77.2182;InbreedingCoeff=-0.0982;MQ=47.37;MQ0=0;MQRankSum=-3.071;QD=1.92;ReadPosRankSum=-7.075;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225507 . C T 63.16 . AC=5;AF=0.0439;AN=114;BaseQRankSum=0.195;DP=525;Dels=0.01;FS=0.000;HRun=1;HaplotypeScore=2.6528;InbreedingCoeff=-0.0729;MQ=47.37;MQ0=0;MQRankSum=-0.118;QD=1.58;ReadPosRankSum=1.001;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225512 . G A 59.61 . AC=5;AF=0.0431;AN=116;BaseQRankSum=0.671;DP=562;Dels=0.01;FS=0.000;HRun=0;HaplotypeScore=2.9722;InbreedingCoeff=-0.0588;MQ=48.31;MQ0=0;MQRankSum=-3.136;QD=1.24;ReadPosRankSum=-1.130;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225517 . T C 31.29 . AC=10;AF=0.0862;AN=116;BaseQRankSum=-0.543;DP=597;Dels=0.00;FS=8.293;HRun=1;HaplotypeScore=2.0472;InbreedingCoeff=0.0340;MQ=48.76;MQ0=0;MQRankSum=-2.154;QD=0.33;ReadPosRankSum=-1.343;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225522 . T C 265.0 . AC=14;AF=0.1207;AN=116;BaseQRankSum=-0.678;DP=629;Dels=0.00;FS=97.747;HRun=2;HaplotypeScore=1.7833;InbreedingCoeff=-0.1442;MQ=49.06;MQ0=0;MQRankSum=-5.490;QD=1.44;ReadPosRankSum=-0.496;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225526 . T TCC 411.2 . AC=10;AF=0.0862;AN=116;BaseQRankSum=4.261;DP=667;FS=14.873;HRun=3;HaplotypeScore=89.4036;InbreedingCoeff=-0.1134;MQ=49.86;MQ0=0;MQRankSum=-3.366;QD=3.29;ReadPosRankSum=-3.904;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225535 . T C 848.26 . AC=10;AF=0.0862;AN=116;BaseQRankSum=-1.442;DP=750;Dels=0.00;FS=4.302;HRun=1;HaplotypeScore=1.3486;InbreedingCoeff=-0.1129;MQ=50.86;MQ0=0;MQRankSum=1.251;QD=5.89;ReadPosRankSum=-1.457;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225540 . T C 45.1 . AC=3;AF=0.0259;AN=116;BaseQRankSum=-1.838;DP=782;Dels=0.00;FS=14.682;HRun=2;HaplotypeScore=1.0795;InbreedingCoeff=-0.0391;MQ=51.27;MQ0=0;MQRankSum=-3.072;QD=2.37;ReadPosRankSum=-2.538;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225543 . G C 72.05 . AC=1;AF=0.0086;AN=116;BaseQRankSum=1.703;DP=821;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=1.7205;InbreedingCoeff=-0.0188;MQ=51.72;MQ0=0;MQRankSum=1.023;QD=3.79;ReadPosRankSum=-1.656;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225544 . T TCC 104.04 . AC=3;AF=0.0263;AN=114;BaseQRankSum=5.030;DP=839;FS=11.849;HRun=3;HaplotypeScore=111.7887;InbreedingCoeff=-0.0353;MQ=51.88;MQ0=0;MQRankSum=-4.162;QD=3.15;ReadPosRankSum=-3.869;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225553 . C T 5273.44 . AC=32;AF=0.2807;AN=114;BaseQRankSum=1.380;DP=911;Dels=0.00;FS=0.497;HRun=0;HaplotypeScore=1.8119;InbreedingCoeff=-0.1881;MQ=52.16;MQ0=0;MQRankSum=2.230;QD=11.39;ReadPosRankSum=-0.044;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225558 . T C 207.02 . AC=1;AF=0.0086;AN=116;BaseQRankSum=0.020;DP=961;Dels=0.00;FS=8.305;HRun=2;HaplotypeScore=1.2090;InbreedingCoeff=-0.0183;MQ=52.59;MQ0=0;MQRankSum=-0.514;QD=12.94;ReadPosRankSum=1.515;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225562 . T TCC 147.67 . AC=5;AF=0.0417;AN=120;BaseQRankSum=3.446;DP=1028;FS=15.375;HRun=3;HaplotypeScore=125.4377;InbreedingCoeff=0.2845;MQ=53.39;MQ0=0;MQRankSum=-2.465;QD=3.14;ReadPosRankSum=-3.790;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225571 . C T 5357.09 . AC=38;AF=0.3167;AN=120;BaseQRankSum=-0.715;DP=1167;Dels=0.00;FS=5.301;HRun=0;HaplotypeScore=2.7736;InbreedingCoeff=0.0326;MQ=54.57;MQ0=0;MQRankSum=-3.297;QD=10.50;ReadPosRankSum=-0.766;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225576 . T C 4461.84 . AC=22;AF=0.1833;AN=120;BaseQRankSum=2.082;DP=1262;Dels=0.00;FS=21.631;HRun=2;HaplotypeScore=2.8144;InbreedingCoeff=0.1776;MQ=54.78;MQ0=0;MQRankSum=0.294;QD=13.48;ReadPosRankSum=0.090;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225579 . G C 1702.39 . AC=10;AF=0.0833;AN=120;BaseQRankSum=1.093;DP=1332;Dels=0.00;FS=11.111;HRun=2;HaplotypeScore=2.9711;InbreedingCoeff=0.0608;MQ=55.00;MQ0=0;MQRankSum=0.576;QD=11.74;ReadPosRankSum=0.329;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225597 . G A 764.88 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.619;DP=1769;Dels=0.00;FS=3.258;HRun=0;HaplotypeScore=1.9881;InbreedingCoeff=-0.0171;MQ=55.96;MQ0=0;MQRankSum=3.394;QD=15.61;ReadPosRankSum=2.984;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225604 . A G 288.23 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.165;DP=1945;Dels=0.00;FS=50.790;HRun=0;HaplotypeScore=2.6125;InbreedingCoeff=-0.0085;MQ=56.12;MQ0=0;MQRankSum=-4.687;QD=13.10;ReadPosRankSum=-3.197;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225612 . G A 8878.59 . AC=25;AF=0.2083;AN=120;BaseQRankSum=-2.436;DP=2093;Dels=0.00;FS=21.815;HRun=0;HaplotypeScore=1.7123;InbreedingCoeff=0.1407;MQ=56.18;MQ0=0;MQRankSum=1.022;QD=13.60;ReadPosRankSum=-1.768;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225641 . C A 16235.21 . AC=27;AF=0.2250;AN=120;BaseQRankSum=-0.237;DP=2629;Dels=0.00;FS=1.703;HRun=0;HaplotypeScore=1.7639;InbreedingCoeff=0.3777;MQ=56.48;MQ0=0;MQRankSum=2.792;QD=20.32;ReadPosRankSum=2.777;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225804 . A G 641.95 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.178;DP=3135;Dels=0.00;FS=4.019;HRun=0;HaplotypeScore=3.7390;InbreedingCoeff=-0.0085;MQ=57.48;MQ0=0;MQRankSum=2.112;QD=13.10;ReadPosRankSum=1.189;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|10),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|9),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|15),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|10),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|13),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|12);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225810 . C CGG 5006.7 . AC=3;AF=0.0250;AN=120;BaseQRankSum=1.861;DP=3131;FS=0.360;HRun=6;HaplotypeScore=237.1014;InbreedingCoeff=0.6361;MQ=57.43;MQ0=0;MQRankSum=-0.361;QD=33.60;ReadPosRankSum=-1.506;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|10),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|9),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|15),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|10),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|13),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|12);CSQ=intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225824 . G A 1475.6 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.326;DP=3390;Dels=0.00;FS=1.074;HRun=1;HaplotypeScore=2.3868;InbreedingCoeff=-0.0086;MQ=57.43;MQ0=0;MQRankSum=2.353;QD=12.20;ReadPosRankSum=-2.766;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|10),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|9),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|15),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|10),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|13),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|12);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225945 . G C 3949.71 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-1.101;DP=3777;Dels=0.00;FS=22.292;HRun=0;HaplotypeScore=4.0017;InbreedingCoeff=0.6569;MQ=57.83;MQ0=0;MQRankSum=-0.665;QD=25.32;ReadPosRankSum=2.508;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|11),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|10),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|16),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|11),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|14),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|13);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1225959 . C G 25993.98 . AC=36;AF=0.3000;AN=120;BaseQRankSum=1.580;DP=3659;Dels=0.00;FS=21.530;HRun=2;HaplotypeScore=2.4662;InbreedingCoeff=0.2055;MQ=57.98;MQ0=0;MQRankSum=1.714;QD=17.82;ReadPosRankSum=0.723;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|11),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|10),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|16),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|11),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|14),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|13);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1226063 . G A 1804.82 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-2.166;DP=2009;Dels=0.00;FS=1.053;HRun=0;HaplotypeScore=1.1275;InbreedingCoeff=-0.0436;MQ=58.53;MQ0=0;MQRankSum=0.440;QD=9.35;ReadPosRankSum=-0.405;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A472T|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A472T|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A503T|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A538T|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A636T|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=missense_variant|Gcc/Acc|A/T|ENSG00000162572|SCNN1D|ENST00000338555|12/15|benign(0.168)|tolerated(0.19)|472/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|14/17|||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,missense_variant|Gcc/Acc|A/T|ENSG00000162572|SCNN1D|ENST00000325425|12/15|benign(0.109)|tolerated(0.39)|538/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,missense_variant|Gcc/Acc|A/T|ENSG00000162572|SCNN1D|ENST00000379099|6/7|benign(0.297)|tolerated(0.13)|289/308|protein_coding|1,missense_variant|Gcc/Acc|A/T|ENSG00000162572|SCNN1D|ENST00000400928|11/14|benign(0.168)|tolerated(0.19)|472/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,missense_variant|Gcc/Acc|A/T|ENSG00000162572|SCNN1D|ENST00000379116|15/18|benign(0.109)|tolerated(0.29)|636/802|protein_coding|1 +chr1 1226102 . G A 4016.45 . AC=11;AF=0.0917;AN=120;BaseQRankSum=-1.245;DP=1541;Dels=0.00;FS=2.281;HRun=0;HaplotypeScore=1.2555;InbreedingCoeff=0.2951;MQ=58.35;MQ0=0;MQRankSum=0.176;QD=16.67;ReadPosRankSum=2.379;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|12),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|11),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|17),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|12),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|15),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1226139 . G A 53.13 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.435;DP=1276;Dels=0.00;FS=8.998;HRun=1;HaplotypeScore=0.7541;InbreedingCoeff=-0.0093;MQ=58.09;MQ0=0;MQRankSum=-1.197;QD=2.95;ReadPosRankSum=-0.731;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|12),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|11),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|17),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|12),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|15),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1226217 . A G 385.18 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.713;DP=1445;Dels=0.00;FS=1.054;HRun=0;HaplotypeScore=1.4065;InbreedingCoeff=-0.0114;MQ=58.08;MQ0=0;MQRankSum=0.322;QD=12.84;ReadPosRankSum=0.561;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|12),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|11),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|17),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|12),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|15),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1226221 . A G 12961.74 . AC=36;AF=0.3000;AN=120;BaseQRankSum=0.407;DP=1473;Dels=0.00;FS=8.750;HRun=2;HaplotypeScore=1.9078;InbreedingCoeff=0.3380;MQ=58.10;MQ0=0;MQRankSum=0.672;QD=20.00;ReadPosRankSum=-0.137;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|12),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|11),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|17),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|12),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|15),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1226231 . G A 1060.58 . AC=4;AF=0.0333;AN=120;BaseQRankSum=0.134;DP=1522;Dels=0.00;FS=3.076;HRun=0;HaplotypeScore=2.2801;InbreedingCoeff=-0.0375;MQ=58.05;MQ0=0;MQRankSum=1.141;QD=13.26;ReadPosRankSum=0.602;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|12),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|11),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|17),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|12),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|15),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1226233 . G A 432.75 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.014;DP=1544;Dels=0.00;FS=11.621;HRun=0;HaplotypeScore=1.7754;InbreedingCoeff=-0.0115;MQ=58.14;MQ0=0;MQRankSum=1.437;QD=9.21;ReadPosRankSum=-0.335;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|12),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|11),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|17),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|12),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|15),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1226246 . C T 658.46 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.957;DP=1671;Dels=0.00;FS=1.750;HRun=0;HaplotypeScore=1.4315;InbreedingCoeff=-0.0192;MQ=58.19;MQ0=0;MQRankSum=1.240;QD=9.98;ReadPosRankSum=-0.155;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|12),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|11),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|17),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|12),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|15),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1226267 . G A 198.26 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.326;DP=1848;Dels=0.00;FS=1.356;HRun=0;HaplotypeScore=0.8439;InbreedingCoeff=-0.0108;MQ=58.43;MQ0=0;MQRankSum=-0.958;QD=8.62;ReadPosRankSum=1.398;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|12),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|11),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|17),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|12),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|15),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1226292 . G A 2311.15 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-0.197;DP=2007;Dels=0.00;FS=0.860;HRun=0;HaplotypeScore=1.1337;InbreedingCoeff=-0.0381;MQ=58.08;MQ0=0;MQRankSum=0.237;QD=11.79;ReadPosRankSum=1.765;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),SYNONYMOUS_CODING(LOW|SILENT|acG/acA|T481|638|SCNN1D|protein_coding|CODING|ENST00000338555|),SYNONYMOUS_CODING(LOW|SILENT|acG/acA|T481|638|SCNN1D|protein_coding|CODING|ENST00000400928|),SYNONYMOUS_CODING(LOW|SILENT|acG/acA|T512|669|SCNN1D|protein_coding|CODING|ENST00000379110|),SYNONYMOUS_CODING(LOW|SILENT|acG/acA|T547|704|SCNN1D|protein_coding|CODING|ENST00000325425|),SYNONYMOUS_CODING(LOW|SILENT|acG/acA|T645|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|15);CSQ=synonymous_variant|acG/acA|T|ENSG00000162572|SCNN1D|ENST00000338555|13/15|||481/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|15/17|||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,synonymous_variant|acG/acA|T|ENSG00000162572|SCNN1D|ENST00000325425|13/15|||547/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,synonymous_variant|acG/acA|T|ENSG00000162572|SCNN1D|ENST00000379099|7/7|||298/308|protein_coding|1,synonymous_variant|acG/acA|T|ENSG00000162572|SCNN1D|ENST00000400928|12/14|||481/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,synonymous_variant|acG/acA|T|ENSG00000162572|SCNN1D|ENST00000379116|16/18|||645/802|protein_coding|1 +chr1 1226348 . C G 14565.59 . AC=27;AF=0.2250;AN=120;BaseQRankSum=-4.148;DP=2185;Dels=0.00;FS=12.103;HRun=0;HaplotypeScore=3.8823;InbreedingCoeff=0.3031;MQ=57.72;MQ0=0;MQRankSum=-0.548;QD=19.76;ReadPosRankSum=2.686;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|13),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|12),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|18),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|13),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|16),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|15);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1226350 . C T 913.84 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.958;DP=2174;Dels=0.00;FS=1.375;HRun=0;HaplotypeScore=3.4324;InbreedingCoeff=-0.0112;MQ=57.70;MQ0=0;MQRankSum=0.687;QD=12.52;ReadPosRankSum=2.553;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|13),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|12),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|18),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|13),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|16),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|15);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1226418 . C T 612.71 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.522;DP=1764;Dels=0.00;FS=13.559;HRun=0;HaplotypeScore=1.3627;InbreedingCoeff=-0.0097;MQ=57.73;MQ0=0;MQRankSum=0.737;QD=13.93;ReadPosRankSum=-1.089;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|13),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|12),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|18),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|13),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|16),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|15);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1226419 . G A 122.55 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.320;DP=1752;Dels=0.00;FS=6.374;HRun=0;HaplotypeScore=1.2212;InbreedingCoeff=-0.0099;MQ=57.75;MQ0=0;MQRankSum=0.393;QD=5.33;ReadPosRankSum=0.165;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|13),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|12),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|18),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|13),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|16),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|15);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1226423 . T C 2109.1 . AC=4;AF=0.0333;AN=120;BaseQRankSum=1.172;DP=1776;Dels=0.00;FS=0.924;HRun=3;HaplotypeScore=1.1033;InbreedingCoeff=0.4687;MQ=57.78;MQ0=0;MQRankSum=3.304;QD=24.24;ReadPosRankSum=0.918;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|13),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|12),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|18),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|13),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|16),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|15);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1226512 . G A 620.19 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.007;DP=1562;Dels=0.00;FS=9.380;HRun=0;HaplotypeScore=0.9448;InbreedingCoeff=-0.0116;MQ=58.77;MQ0=0;MQRankSum=0.069;QD=24.81;ReadPosRankSum=-2.040;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V518M|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V518M|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V549M|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V584M|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V682M|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|16);CSQ=missense_variant|Gtg/Atg|V/M|ENSG00000162572|SCNN1D|ENST00000338555|14/15|benign(0.109)|tolerated(0.11)|518/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|16/17|||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,missense_variant|Gtg/Atg|V/M|ENSG00000162572|SCNN1D|ENST00000325425|14/15|benign(0.036)|tolerated(0.12)|584/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,missense_variant|Gtg/Atg|V/M|ENSG00000162572|SCNN1D|ENST00000400928|13/14|benign(0.109)|tolerated(0.11)|518/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,missense_variant|Gtg/Atg|V/M|ENSG00000162572|SCNN1D|ENST00000379116|17/18|benign(0.036)|tolerated(0.1)|682/802|protein_coding|1 +chr1 1226539 . C T 817.48 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-2.085;DP=1317;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6593;InbreedingCoeff=-0.0489;MQ=58.71;MQ0=0;MQRankSum=0.576;QD=13.62;ReadPosRankSum=0.439;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|14),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|13),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|19),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|14),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|17),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|16);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 +chr1 1226651 . G C 418.81 . AC=5;AF=0.0417;AN=120;BaseQRankSum=2.115;DP=667;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.3223;InbreedingCoeff=-0.0562;MQ=58.20;MQ0=0;MQRankSum=0.758;QD=9.74;ReadPosRankSum=-0.561;EFF=DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),SYNONYMOUS_CODING(LOW|SILENT|tcG/tcC|S526|638|SCNN1D|protein_coding|CODING|ENST00000338555|),SYNONYMOUS_CODING(LOW|SILENT|tcG/tcC|S526|638|SCNN1D|protein_coding|CODING|ENST00000400928|),SYNONYMOUS_CODING(LOW|SILENT|tcG/tcC|S557|669|SCNN1D|protein_coding|CODING|ENST00000379110|),SYNONYMOUS_CODING(LOW|SILENT|tcG/tcC|S592|704|SCNN1D|protein_coding|CODING|ENST00000325425|),SYNONYMOUS_CODING(LOW|SILENT|tcG/tcC|S690|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|17);CSQ=synonymous_variant|tcG/tcC|S|ENSG00000162572|SCNN1D|ENST00000338555|15/15|||526/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|17/17|||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,synonymous_variant|tcG/tcC|S|ENSG00000162572|SCNN1D|ENST00000325425|15/15|||592/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,synonymous_variant|tcG/tcC|S|ENSG00000162572|SCNN1D|ENST00000400928|14/14|||526/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,synonymous_variant|tcG/tcC|S|ENSG00000162572|SCNN1D|ENST00000379116|18/18|||690/802|protein_coding|1 +chr1 1226690 . C T 282.98 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.735;DP=758;Dels=0.00;FS=19.978;HRun=0;HaplotypeScore=0.3775;InbreedingCoeff=-0.0239;MQ=58.31;MQ0=0;MQRankSum=0.192;QD=12.30;ReadPosRankSum=0.815;EFF=DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),SYNONYMOUS_CODING(LOW|SILENT|tcC/tcT|S539|638|SCNN1D|protein_coding|CODING|ENST00000338555|),SYNONYMOUS_CODING(LOW|SILENT|tcC/tcT|S539|638|SCNN1D|protein_coding|CODING|ENST00000400928|),SYNONYMOUS_CODING(LOW|SILENT|tcC/tcT|S570|669|SCNN1D|protein_coding|CODING|ENST00000379110|),SYNONYMOUS_CODING(LOW|SILENT|tcC/tcT|S605|704|SCNN1D|protein_coding|CODING|ENST00000325425|),SYNONYMOUS_CODING(LOW|SILENT|tcC/tcT|S703|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|17);CSQ=synonymous_variant|tcC/tcT|S|ENSG00000162572|SCNN1D|ENST00000338555|15/15|||539/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|17/17|||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,synonymous_variant|tcC/tcT|S|ENSG00000162572|SCNN1D|ENST00000325425|15/15|||605/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,synonymous_variant|tcC/tcT|S|ENSG00000162572|SCNN1D|ENST00000400928|14/14|||539/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,synonymous_variant|tcC/tcT|S|ENSG00000162572|SCNN1D|ENST00000379116|18/18|||703/802|protein_coding|1 +chr1 1226757 . G A 1577.2 . AC=12;AF=0.1000;AN=120;BaseQRankSum=-2.078;DP=939;Dels=0.00;FS=1.648;HRun=0;HaplotypeScore=0.5032;InbreedingCoeff=-0.1091;MQ=57.89;MQ0=0;MQRankSum=0.038;QD=8.76;ReadPosRankSum=0.677;EFF=DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Agc|G562S|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Agc|G562S|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Agc|G593S|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Agc|G628S|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Agc|G726S|802|SCNN1D|protein_coding|CODING|ENST00000379116|);CSQ=missense_variant|Ggc/Agc|G/S|ENSG00000162572|SCNN1D|ENST00000338555|15/15|benign(0.125)|tolerated(0.39)|562/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,missense_variant|Ggc/Agc|G/S|ENSG00000162572|SCNN1D|ENST00000325425|15/15|benign(0.276)|tolerated(0.4)|628/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,missense_variant|Ggc/Agc|G/S|ENSG00000162572|SCNN1D|ENST00000400928|14/14|benign(0.125)|tolerated(0.39)|562/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,missense_variant|Ggc/Agc|G/S|ENSG00000162572|SCNN1D|ENST00000379116|18/18|benign(0.276)|tolerated(0.4)|726/802|protein_coding|1 +chr1 1226851 . C T 188.21 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.990;DP=1115;Dels=0.00;FS=5.214;HRun=0;HaplotypeScore=0.5651;InbreedingCoeff=-0.0104;MQ=58.68;MQ0=0;MQRankSum=-1.264;QD=9.41;ReadPosRankSum=-0.899;EFF=DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|processed_transcript|CODING|ENST00000379037|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000476572|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P593L|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P593L|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P624L|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P659L|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P757L|802|SCNN1D|protein_coding|CODING|ENST00000379116|);CSQ=missense_variant|cCg/cTg|P/L|ENSG00000162572|SCNN1D|ENST00000338555|15/15|benign(0.007)|deleterious(0.05)|593/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000476572|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000379037|||||processed_transcript|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,missense_variant|cCg/cTg|P/L|ENSG00000162572|SCNN1D|ENST00000325425|15/15|benign(0.005)|tolerated(0.09)|659/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,missense_variant|cCg/cTg|P/L|ENSG00000162572|SCNN1D|ENST00000400928|14/14|benign(0.007)|deleterious(0.05)|593/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,missense_variant|cCg/cTg|P/L|ENSG00000162572|SCNN1D|ENST00000379116|18/18|benign(0.005)|deleterious(0.02)|757/802|protein_coding|1 +chr1 1226889 . G A 2836.8 . AC=12;AF=0.1000;AN=120;BaseQRankSum=-2.929;DP=1114;Dels=0.00;FS=6.315;HRun=0;HaplotypeScore=0.3672;InbreedingCoeff=-0.1141;MQ=58.73;MQ0=0;MQRankSum=0.148;QD=15.76;ReadPosRankSum=0.535;EFF=DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|processed_transcript|CODING|ENST00000379037|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000476572|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggg/Agg|G606R|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggg/Agg|G606R|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggg/Agg|G637R|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggg/Agg|G672R|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggg/Agg|G770R|802|SCNN1D|protein_coding|CODING|ENST00000379116|);CSQ=missense_variant|Ggg/Agg|G/R|ENSG00000162572|SCNN1D|ENST00000338555|15/15|benign(0.159)|tolerated(0.42)|606/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000476572|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000379037|||||processed_transcript|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,missense_variant|Ggg/Agg|G/R|ENSG00000162572|SCNN1D|ENST00000325425|15/15|benign(0.001)|tolerated(0.38)|672/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,missense_variant|Ggg/Agg|G/R|ENSG00000162572|SCNN1D|ENST00000400928|14/14|benign(0.159)|tolerated(0.42)|606/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,missense_variant|Ggg/Agg|G/R|ENSG00000162572|SCNN1D|ENST00000379116|18/18|benign(0.001)|tolerated(0.08)|770/802|protein_coding|1 +chr1 1226971 . T C 63.36 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-1.160;DP=1268;Dels=0.00;FS=4.729;HRun=4;HaplotypeScore=0.4672;InbreedingCoeff=-0.0189;MQ=58.51;MQ0=0;MQRankSum=1.085;QD=3.73;ReadPosRankSum=-0.628;EFF=DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|processed_transcript|CODING|ENST00000379037|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000476572|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cTt/cCt|L633P|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cTt/cCt|L633P|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cTt/cCt|L664P|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cTt/cCt|L699P|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cTt/cCt|L797P|802|SCNN1D|protein_coding|CODING|ENST00000379116|);CSQ=missense_variant|cTt/cCt|L/P|ENSG00000162572|SCNN1D|ENST00000338555|15/15|benign(0.02)|tolerated(0.18)|633/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000476572|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000379037|||||processed_transcript|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,missense_variant|cTt/cCt|L/P|ENSG00000162572|SCNN1D|ENST00000325425|15/15|benign(0.189)|tolerated(0.21)|699/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,missense_variant|cTt/cCt|L/P|ENSG00000162572|SCNN1D|ENST00000400928|14/14|benign(0.02)|tolerated(0.18)|633/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,missense_variant|cTt/cCt|L/P|ENSG00000162572|SCNN1D|ENST00000379116|18/18|benign(0.189)|deleterious(0.04)|797/802|protein_coding|1 +chr1 1227042 . G A 57.76 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.393;DP=957;Dels=0.00;FS=2.494;HRun=0;HaplotypeScore=0.5259;InbreedingCoeff=-0.0359;MQ=58.61;MQ0=0;MQRankSum=0.096;QD=14.44;ReadPosRankSum=2.314;EFF=DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|processed_transcript|CODING|ENST00000379037|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000476572|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|),UTR_3_PRIME(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|15),UTR_3_PRIME(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|14),UTR_3_PRIME(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|20),UTR_3_PRIME(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|15),UTR_3_PRIME(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|18);CSQ=3_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000338555|15/15|||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000476572|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000379037|||||processed_transcript|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,3_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000325425|15/15|||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,3_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000400928|14/14|||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000379116|18/18|||-/802|protein_coding|1 +chr1 1227196 . A G 1166.6 . AC=2;AF=0.024;AN=82;BaseQRankSum=1.195;DP=1062;Dels=0.00;FS=10.378;HRun=6;HaplotypeScore=1.5050;InbreedingCoeff=-0.0572;MQ=57.57;MQ0=0;MQRankSum=-0.308;QD=10.42;ReadPosRankSum=0.286;EFF=DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|processed_transcript|CODING|ENST00000379037|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000476572|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|),UTR_3_PRIME(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|15),UTR_3_PRIME(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|14),UTR_3_PRIME(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|20),UTR_3_PRIME(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|15),UTR_3_PRIME(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|18);CSQ=3_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000338555|15/15|||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000476572|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000379037|||||processed_transcript|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,3_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000325425|15/15|||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,3_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000400928|14/14|||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000379116|18/18|||-/802|protein_coding|1 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zr*GLEA1SBp^xJ#lcgkryUGl5=XgO`CFWeu$TTa{QLEps3$!R;i^gw))oVL^NAB^88 zr|tA--^TBk({{Sfq4;DuZKtn35`R!m+vyqq8=oSl?ezP{;#1|coo-YjdrPOwX*=Do zWPFC4w$ocm$Nwm&?R3?j#UGW^cDhpK_*^+{r!T1*pC_m7^p7>-^X0UiKBHFraXD?L zZ>SSrAgAs0{Ce?)a@tOBtsh?`r|mR5VwcKkJI&tMXXWgUwbQpP%s$uW)H4|Ic=x6uZka$({}oz)$yZp+D;FC zDSk{&+vzV}i61v#--#-;ovynkUQ$lm>0WE&KbO;XdegdiMLBJ!_k0>ZQBK?G$)CsT z$Z0$M&dzu}Ic=xeF?*Vvw$tpL{gs^Evvzvbq1@k0PTT2ihvUuVw4FY1B!0e}w$s1* zKHf@B+v!_>h_{o|cDi55rvLQ5;0xunojy<|-pTJTbn*KO-MC!5vz)fmiz~!?$UiLi z?|q|m#qxR9Q%?Il-TTD&Wpdh1Z>SuT2<;)8pZp|X*+#SllVY6ZKvNlD?V8MLW{JW zKC5+}4UyA!df)}|p>oeC#UW7U#^QkAgAqgg}(8J<+PpddP97woVL^V-WZ=Qr|tCi0r44f z+D>0SC_Z0K+v&x(#TUqFJ6-mU_+mM2r&kV*KP{*2^xom|6>{26H@q{xQcm0HS!3eQ z$Z0#h<(~K|Ic=vakB>hmr|opZ3GwIs{z4bOztG(##$S-rc6#!p_)BuyPOrW%{)(Ko z(?t)&*T`u*eal1fH{`UPUi@%;y_~kwCryk0RZiRK$_3r|tCC1@YZ-+D Date: Wed, 30 Aug 2023 11:31:40 -0700 Subject: [PATCH 098/116] Allow name panel width settings > 1000 pixels --- src/main/java/org/broad/igv/ui/IGVMenuBar.java | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/src/main/java/org/broad/igv/ui/IGVMenuBar.java b/src/main/java/org/broad/igv/ui/IGVMenuBar.java index aca257bf9f..20984bee6a 100644 --- a/src/main/java/org/broad/igv/ui/IGVMenuBar.java +++ b/src/main/java/org/broad/igv/ui/IGVMenuBar.java @@ -595,11 +595,11 @@ public void actionPerformed(ActionEvent e) { if (newValue != null) { try { Integer w = Integer.parseInt(newValue); - if (w <= 0 || w == 1000) throw new NumberFormatException(); + if (w <= 0) throw new NumberFormatException(); PreferencesManager.getPreferences().put(NAME_PANEL_WIDTH, newValue); mainPanel.setNamePanelWidth(w); } catch (NumberFormatException ex) { - MessageUtils.showErrorMessage("Error: value must be a positive integer < 1000.", ex); + MessageUtils.showErrorMessage("Error: value must be a positive integer.", ex); } } } From d0fcdd3093492a5d5c9b4ba636e46e31ce36b9f2 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 30 Aug 2023 16:37:16 -0700 Subject: [PATCH 099/116] Combine linked-reads menu items. Fix right-clip sequence bug. --- .../java/org/broad/igv/sam/AlignmentTrackMenu.java | 12 +++++++----- 1 file changed, 7 insertions(+), 5 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index 26cbe2dff9..cedb8acd8b 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -962,7 +962,7 @@ void addCopySequenceItems(final TrackClickEvent te) { String rcSeq = getClippedSequence( alignment.getReadSequence(), alignment.getReadStrand(), - seqLength - clipping.getRightHard(), + seqLength - clipping.getRightSoft(), seqLength); final JMenuItem rccItem = new JMenuItem("Copy right-clipped sequence"); @@ -1076,8 +1076,6 @@ void addExtViewItem(final TrackClickEvent te) { */ void addLinkedReadItems() { addSeparator(); - add(linkedReadViewItem("BX")); - add(linkedReadViewItem("MI")); addSeparator(); final JCheckBoxMenuItem supplementalItem = new JCheckBoxMenuItem("Link supplementary alignments"); @@ -1130,7 +1128,11 @@ private JCheckBoxMenuItem linkedReadItem(String tag) { item.setSelected(!alignmentTrack.isLinkedReadView() && alignmentTrack.isLinkedReads() && tag.equals(renderOptions.getLinkByTag())); item.addActionListener(aEvt -> { boolean linkedReads = item.isSelected(); - setLinkByTag(linkedReads, tag); + if("BX".equals(tag) || "MI".equals(tag)) { + alignmentTrack.setLinkedReadView(linkedReads, tag); + } else { + setLinkByTag(linkedReads, tag); + } }); return item; } @@ -1355,7 +1357,7 @@ private void setLinkByTag(boolean linkReads, String tag) { } renderOptions.setLinkedReads(linkReads); dataManager.packAlignments(renderOptions); - repaint(); + alignmentTrack.repaint(); } From 58c23a12bf22ae83328e60d5ebfe0bc88f024f32 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 30 Aug 2023 17:19:04 -0700 Subject: [PATCH 100/116] Revert "Feature refactor (#1382)" This reverts commit 361cde8b96349fbdfee2bec00579e9017220f134. --- src/main/java/module-info.java | 2 - .../org/broad/igv/bbfile/BBDataSource.java | 2 +- .../java/org/broad/igv/data/DataSource.java | 7 +- .../igv/data/seg/SegmentedDataSource.java | 5 + .../org/broad/igv/feature/AbstractCacher.java | 365 ++++++ .../igv/feature/CachingFeatureSource.java | 93 ++ .../org/broad/igv/feature/IGVFeature.java | 4 +- .../java/org/broad/igv/feature/WGFeature.java | 57 - .../igv/feature/gff/GFFFeatureSource.java | 4 +- .../igv/feature/tribble/IGVFeatureReader.java | 16 +- .../broad/igv/feature/tribble/MUTCodec.java | 10 +- .../igv/feature/tribble/reader/BamIndex.java | 310 ----- .../igv/feature/tribble/reader/CSIIndex.java | 328 ------ .../igv/feature/tribble/reader/VPointer.java | 29 - .../broad/igv/htsget/HtsgetVariantSource.java | 2 +- .../broad/igv/session/IGVSessionReader.java | 2 - .../tools/motiffinder/MotifFinderPlugin.java | 5 + .../tools/motiffinder/MotifFinderSource.java | 5 +- .../igv/track/FeatureCollectionSource.java | 28 +- .../org/broad/igv/track/FeatureSource.java | 22 +- .../org/broad/igv/track/FeatureTrack.java | 124 +- .../java/org/broad/igv/track/MotifTrack.java | 6 +- .../org/broad/igv/track/MutationTrack.java | 78 ++ .../broad/igv/track/TribbleFeatureSource.java | 19 +- .../igv/track/TribbleListFeatureSource.java | 2 +- .../org/broad/igv/variant/New/Variant.java | 19 +- .../org/broad/igv/prefs/preferences.tab | 2 - .../feature/tribble/reader/BamIndexTest.java | 171 --- .../feature/tribble/reader/CSIIndexTest.java | 94 -- test/data/tabix/TSVC.vcf.gz | Bin 4833 -> 0 bytes test/data/tabix/TSVC.vcf.gz.csi | Bin 140 -> 0 bytes test/data/tabix/TSVC.vcf.gz.tbi | Bin 135 -> 0 bytes test/data/tabix/csi-test.vcf | 1001 ----------------- test/data/tabix/csi-test.vcf.gz | Bin 94832 -> 0 bytes test/data/tabix/csi-test.vcf.gz.csi | Bin 326 -> 0 bytes test/data/tabix/index_test.bam.csi | Bin 48824 -> 0 bytes test/data/tabix/long_references.bam.csi | Bin 133 -> 0 bytes test/data/tabix/noindex.chr1.gff.gz | Bin 313 -> 0 bytes test/data/tabix/refGene.hg19.bed.gz.tbi | Bin 106571 -> 0 bytes test/data/tabix/sorted.genes.gtf.gz | Bin 5207306 -> 0 bytes test/data/tabix/sorted.genes.gtf.gz.csi | Bin 37288 -> 0 bytes test/data/tabix/sorted.genes.gtf.gz.tbi | Bin 245605 -> 0 bytes test/data/tabix/uncompressed_index.bam.csi | Bin 6623684 -> 0 bytes 43 files changed, 666 insertions(+), 2146 deletions(-) create mode 100644 src/main/java/org/broad/igv/feature/AbstractCacher.java create mode 100644 src/main/java/org/broad/igv/feature/CachingFeatureSource.java delete mode 100644 src/main/java/org/broad/igv/feature/WGFeature.java delete mode 100644 src/main/java/org/broad/igv/feature/tribble/reader/BamIndex.java delete mode 100644 src/main/java/org/broad/igv/feature/tribble/reader/CSIIndex.java delete mode 100644 src/main/java/org/broad/igv/feature/tribble/reader/VPointer.java delete mode 100644 src/test/java/org/broad/igv/feature/tribble/reader/BamIndexTest.java delete mode 100644 src/test/java/org/broad/igv/feature/tribble/reader/CSIIndexTest.java delete mode 100755 test/data/tabix/TSVC.vcf.gz delete mode 100644 test/data/tabix/TSVC.vcf.gz.csi delete mode 100755 test/data/tabix/TSVC.vcf.gz.tbi delete mode 100644 test/data/tabix/csi-test.vcf delete mode 100644 test/data/tabix/csi-test.vcf.gz delete mode 100644 test/data/tabix/csi-test.vcf.gz.csi delete mode 100644 test/data/tabix/index_test.bam.csi delete mode 100644 test/data/tabix/long_references.bam.csi delete mode 100644 test/data/tabix/noindex.chr1.gff.gz delete mode 100644 test/data/tabix/refGene.hg19.bed.gz.tbi delete mode 100644 test/data/tabix/sorted.genes.gtf.gz delete mode 100644 test/data/tabix/sorted.genes.gtf.gz.csi delete mode 100644 test/data/tabix/sorted.genes.gtf.gz.tbi delete mode 100644 test/data/tabix/uncompressed_index.bam.csi diff --git a/src/main/java/module-info.java b/src/main/java/module-info.java index 9e822e87a7..15bb2a73f2 100644 --- a/src/main/java/module-info.java +++ b/src/main/java/module-info.java @@ -5,10 +5,8 @@ exports org.broad.igv.jbrowse; exports org.broad.igv.logging; exports org.broad.igv.util.liftover; - exports org.broad.igv.feature; exports org.broad.igv.sam.smrt; - requires com.google.common; requires commons.math3; requires gson; diff --git a/src/main/java/org/broad/igv/bbfile/BBDataSource.java b/src/main/java/org/broad/igv/bbfile/BBDataSource.java index 36c609e16e..2d19355227 100644 --- a/src/main/java/org/broad/igv/bbfile/BBDataSource.java +++ b/src/main/java/org/broad/igv/bbfile/BBDataSource.java @@ -111,7 +111,7 @@ public BBDataSource(BBFileReader reader, Genome genome) throws IOException { } @Override - public int estimateFeatureWindowSize() { + public int getFeatureWindowSize() { return featureVisiblityWindow; } diff --git a/src/main/java/org/broad/igv/data/DataSource.java b/src/main/java/org/broad/igv/data/DataSource.java index 3df6293ffa..b4efe3778b 100644 --- a/src/main/java/org/broad/igv/data/DataSource.java +++ b/src/main/java/org/broad/igv/data/DataSource.java @@ -60,12 +60,15 @@ List getSummaryScoresForRange( boolean isLogNormalized(); - WindowFunction getWindowFunction(); + public WindowFunction getWindowFunction(); - default Collection getAvailableWindowFunctions() { + public default Collection getAvailableWindowFunctions() { return Collections.EMPTY_LIST; } + default boolean isIndexable() { + return true; + } /** * Release any resources (file handles, etc) diff --git a/src/main/java/org/broad/igv/data/seg/SegmentedDataSource.java b/src/main/java/org/broad/igv/data/seg/SegmentedDataSource.java index 6beed7ad31..654bd4829e 100644 --- a/src/main/java/org/broad/igv/data/seg/SegmentedDataSource.java +++ b/src/main/java/org/broad/igv/data/seg/SegmentedDataSource.java @@ -74,6 +74,11 @@ public void setWindowFunction(WindowFunction statType) { // ignore } + @Override + public boolean isIndexable() { + return false; + } + public boolean isLogNormalized() { return dataset.isLogNormalized(); } diff --git a/src/main/java/org/broad/igv/feature/AbstractCacher.java b/src/main/java/org/broad/igv/feature/AbstractCacher.java new file mode 100644 index 0000000000..f7852565a2 --- /dev/null +++ b/src/main/java/org/broad/igv/feature/AbstractCacher.java @@ -0,0 +1,365 @@ +/* + * The MIT License (MIT) + * + * Copyright (c) 2007-2015 Broad Institute + * + * Permission is hereby granted, free of charge, to any person obtaining a copy + * of this software and associated documentation files (the "Software"), to deal + * in the Software without restriction, including without limitation the rights + * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell + * copies of the Software, and to permit persons to whom the Software is + * furnished to do so, subject to the following conditions: + * + * The above copyright notice and this permission notice shall be included in + * all copies or substantial portions of the Software. + * + * + * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR + * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, + * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE + * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER + * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, + * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN + * THE SOFTWARE. + */ + + +package org.broad.igv.feature; + +import org.broad.igv.logging.*; +import org.broad.igv.util.collections.LRUCache; +import htsjdk.tribble.CloseableTribbleIterator; +import htsjdk.tribble.Feature; + +import java.io.IOException; +import java.util.ArrayList; +import java.util.Collections; +import java.util.Iterator; +import java.util.List; + + +/** + * Class to handle caching data from any source of features + * Subclasses must override queryRaw + * + * @author jrobinso + * @date Jun 24, 2010 + */ +public abstract class AbstractCacher { + + private static Logger log = LogManager.getLogger(AbstractCacher.class); + + protected int binSize = Integer.MAX_VALUE; + protected LRUCache cache; + + + public AbstractCacher(int binCount, int binSize) { + this.cache = new LRUCache(binCount); + setBinSize(binSize); + } + + /** + * Obtain data from underlying source + * + * @param chr + * @param start + * @param end + * @return + */ + protected abstract Iterator queryRaw(String chr, int start, int end) throws IOException; + + + + /** + * Set the bin size. This invalidates the cache. + * + * @param newSize + */ + public void setBinSize(int newSize) { + this.binSize = newSize == 0 ? Integer.MAX_VALUE : newSize; // A binSize of zero => use a single bin for the entire chromosome + cache.clear(); + + } + + public void close() { + cache.clear(); + } + + /** + * Query the cached data, refreshing from raw data as necessary + * + * @param chr + * @param start + * @param end + * @return + * @throws IOException + */ + public Iterator queryCached(String chr, int start, int end) throws IOException { + + int startBin = start / binSize; + int endBin = end / binSize; // <= inclusive + + List tiles = getBins(chr, startBin, endBin); + + if (tiles.size() == 0) { + return Collections.emptyList().iterator(); + } + + // Count total # of records + int recordCount = tiles.get(0).getOverlappingRecords().size(); + for (Bin t : tiles) { + recordCount += t.getContainedRecords().size(); + } + + List features = new ArrayList(recordCount); + features.addAll(tiles.get(0).getOverlappingRecords()); + for (Bin t : tiles) { + features.addAll(t.getContainedRecords()); + } + return new BinIterator(start, end, features); + } + + + /** + * Return loaded tiles that span the query interval. + *

      + * We synchronize this method because different threads might be using + * the same source. Synchronizing here ensures that data + * is loaded as few times as possible (first caller loads it into the cache, + * the second caller accesses it from there) as well as preventing bugs stemming + * from multiple thread access + * + * @param seq + * @param startBin + * @param endBin + * @return + */ + private synchronized List getBins(String seq, int startBin, int endBin) { + + List tiles = new ArrayList(endBin - startBin + 1); + List tilesToLoad = new ArrayList(endBin - startBin + 1); + + for (int t = startBin; t <= endBin; t++) { + String key = seq + "_" + t; + Bin tile = cache.get(key); + + if (tile == null) { + if (log.isDebugEnabled()) { + log.debug("Tile cache miss: " + t); + } + int start = t * binSize; + int end = start + binSize; + tile = new Bin(t, start, end); + cache.put(key, tile); + } + + tiles.add(tile); + + // The current tile is loaded, load any preceding tiles we have pending + if (tile.isLoaded()) { + if (tilesToLoad.size() > 0) { + if (!loadTiles(seq, tilesToLoad)) { + return tiles; + } + } + tilesToLoad.clear(); + } else { + tilesToLoad.add(tile); + } + } + + if (tilesToLoad.size() > 0) { + loadTiles(seq, tilesToLoad); + } + + return tiles; + } + + private boolean loadTiles(String seq, List tiles) { + + assert (tiles.size() > 0); + + if (log.isDebugEnabled()) { + int first = tiles.get(0).getBinNumber(); + int end = tiles.get(tiles.size() - 1).getBinNumber(); + log.debug("Loading tiles: " + first + "-" + end); + } + + // Convert start to 1-based coordinates + int start = tiles.get(0).start + 1; + int end = tiles.get(tiles.size() - 1).end; + Iterator iter = null; + + //log.debug("Loading : " + start + " - " + end); + int featureCount = 0; + long t0 = System.currentTimeMillis(); + try { + + iter = queryRaw(seq, start, end); + + while (iter != null && iter.hasNext()) { + Feature record = iter.next(); + + // Range of tile indices that this feature contributes to. + int aStart = record.getStart(); + int aEnd = record.getEnd(); + int idx0 = 0; + int idx1 = 0; + if (binSize > 0) { + idx0 = Math.max(0, (aStart - start) / binSize); + idx1 = Math.min(tiles.size() - 1, (aEnd - start) / binSize); + } + + // Loop over tiles this read overlaps + for (int i = idx0; i <= idx1; i++) { + Bin t = tiles.get(i); + + // A bin size == 0 means use a single bin for the entire chromosome. This is a confusing convention. + if (binSize == 0 || ((aStart >= t.start) && (aStart < t.end))) { + t.containedRecords.add(record); + } else if ((aEnd >= t.start) && (aStart < t.start)) { + t.overlappingRecords.add(record); + } + } + } + + for (Bin t : tiles) { + t.setLoaded(true); + } + if (log.isDebugEnabled()) { + long dt = System.currentTimeMillis() - t0; + long rate = dt == 0 ? Long.MAX_VALUE : featureCount / dt; + log.debug("Loaded " + featureCount + " reads in " + dt + "ms. (" + rate + " reads/ms)"); + } + return true; + + } catch (IOException e) { + log.error("IOError loading feature data", e); + + // TODO -- do something about this, how do we want to handle this exception? + throw new RuntimeException(e); + } finally { + if (iter != null) { + //iter.close(); + } + //IGV.getInstance().resetStatusMessage(); + } + } + + + private static class Bin { + + private boolean loaded = false; + private int start; + private int end; + private int binNumber; + private List containedRecords; + private List overlappingRecords; + + Bin(int binNumber, int start, int end) { + this.binNumber = binNumber; + this.start = start; + this.end = end; + containedRecords = new ArrayList(1000); + overlappingRecords = new ArrayList(100); + } + + public int getBinNumber() { + return binNumber; + } + + + public int getStart() { + return start; + } + + public void setStart(int start) { + this.start = start; + } + + public List getContainedRecords() { + return containedRecords; + } + + public List getOverlappingRecords() { + return overlappingRecords; + } + + public boolean isLoaded() { + return loaded; + } + + public void setLoaded(boolean loaded) { + this.loaded = loaded; + } + + } + + /** + * + */ + private class BinIterator implements CloseableTribbleIterator { + + Iterator currentFeatureIterator; + int end; + Feature nextRecord; + int start; + List features; + + BinIterator(int start, int end, List features) { + this.features = features; + this.start = start; + this.end = end; + currentFeatureIterator = features.iterator(); + advanceToFirstRecord(); + } + + public void close() { + // No-op + } + + public boolean hasNext() { + return nextRecord != null; + } + + public Feature next() { + Feature ret = nextRecord; + + advanceToNextRecord(); + + return ret; + } + + public void remove() { + // ignored + } + + private void advanceToFirstRecord() { + advanceToNextRecord(); + } + + private void advanceToNextRecord() { + advance(); + + while ((nextRecord != null) && (nextRecord.getEnd() < start)) { + advance(); + } + } + + private void advance() { + if (currentFeatureIterator.hasNext()) { + nextRecord = currentFeatureIterator.next(); + if (nextRecord.getStart() > end) { + nextRecord = null; + } + } else { + nextRecord = null; + } + } + + public Iterator iterator() { + return this; + } + } +} + diff --git a/src/main/java/org/broad/igv/feature/CachingFeatureSource.java b/src/main/java/org/broad/igv/feature/CachingFeatureSource.java new file mode 100644 index 0000000000..407b92486a --- /dev/null +++ b/src/main/java/org/broad/igv/feature/CachingFeatureSource.java @@ -0,0 +1,93 @@ +/* + * The MIT License (MIT) + * + * Copyright (c) 2007-2015 Broad Institute + * + * Permission is hereby granted, free of charge, to any person obtaining a copy + * of this software and associated documentation files (the "Software"), to deal + * in the Software without restriction, including without limitation the rights + * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell + * copies of the Software, and to permit persons to whom the Software is + * furnished to do so, subject to the following conditions: + * + * The above copyright notice and this permission notice shall be included in + * all copies or substantial portions of the Software. + * + * + * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR + * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, + * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE + * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER + * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, + * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN + * THE SOFTWARE. + */ + +package org.broad.igv.feature; + +import org.broad.igv.track.FeatureSource; +import htsjdk.tribble.Feature; + +import java.io.IOException; +import java.util.Iterator; +import java.util.List; + +/** + * A FeatureSource wrapper which provides caching. + * The cache is only cleared when the source is closed. + * + * @author jacob + * @date 2012/05/15 + */ +public class CachingFeatureSource extends AbstractCacher implements FeatureSource { + + private static final int maxBinCount = 1000; + private static final int defaultBinSize = 16000; // <= 16 kb + + private FeatureSource source; + + + /** + * Wraps the provided {@code source} with a caching version, + * using default parameters. + * @param source + * @api + */ + public CachingFeatureSource(FeatureSource source) { + this(source, maxBinCount, defaultBinSize); + } + + + public CachingFeatureSource(FeatureSource source, int binCount, int binSize) { + super(binCount, binSize); + this.source = source; + } + + @Override + protected Iterator queryRaw(String chr, int start, int end) throws IOException { + return source.getFeatures(chr, start, end); + } + + @Override + public Iterator getFeatures(String chr, int start, int end) throws IOException { + return super.queryCached(chr, start, end); + } + + @Override + public List getCoverageScores(String chr, int start, int end, int zoom) { + return source.getCoverageScores(chr, start, end, zoom); + } + + @Override + public int getFeatureWindowSize() { + return source.getFeatureWindowSize(); + } + + /** + * Return the source which backs this CachingFeatureSource + * @return + */ + public FeatureSource getSource(){ + return this.source; + } +} diff --git a/src/main/java/org/broad/igv/feature/IGVFeature.java b/src/main/java/org/broad/igv/feature/IGVFeature.java index 79134f8f8f..0852468bd3 100644 --- a/src/main/java/org/broad/igv/feature/IGVFeature.java +++ b/src/main/java/org/broad/igv/feature/IGVFeature.java @@ -35,7 +35,7 @@ * Interface for features in IGV annotation tracks (FeatureTrack and derived classes). */ -public interface IGVFeature extends LocusScore, NamedFeature, Cloneable { +public interface IGVFeature extends LocusScore, NamedFeature { default String getIdentifier() { return null; @@ -90,7 +90,7 @@ default List getExons() { return null; } - Color getColor(); + public Color getColor(); default String getURL() { return null; diff --git a/src/main/java/org/broad/igv/feature/WGFeature.java b/src/main/java/org/broad/igv/feature/WGFeature.java deleted file mode 100644 index 74bf44e46a..0000000000 --- a/src/main/java/org/broad/igv/feature/WGFeature.java +++ /dev/null @@ -1,57 +0,0 @@ -package org.broad.igv.feature; - -import org.broad.igv.Globals; -import org.broad.igv.feature.genome.GenomeManager; -import org.broad.igv.track.WindowFunction; - -import java.awt.*; - -public class WGFeature implements IGVFeature { - - IGVFeature wrappedFeature; - int start; - int end; - - public WGFeature(IGVFeature wrappedFeature, int start, int end) { - this.wrappedFeature = wrappedFeature; - this.start = start; - this.end = end; - } - - - @Override - public Color getColor() { - return wrappedFeature.getColor(); - } - - @Override - public float getScore() { - return wrappedFeature.getScore(); - } - - @Override - public String getName() { - return wrappedFeature.getName(); - } - - @Override - public String getContig() { - return Globals.CHR_ALL; - } - - @Override - public int getStart() { - return start; - } - - @Override - public int getEnd() { - return end; - } - - @Override - public String getValueString(double position, int mouseX, WindowFunction windowFunction) { - // TODDO -- translate position to chr position - return wrappedFeature.getValueString(0, mouseX, windowFunction); - } -} diff --git a/src/main/java/org/broad/igv/feature/gff/GFFFeatureSource.java b/src/main/java/org/broad/igv/feature/gff/GFFFeatureSource.java index eb72a50188..ff9da6d7e6 100644 --- a/src/main/java/org/broad/igv/feature/gff/GFFFeatureSource.java +++ b/src/main/java/org/broad/igv/feature/gff/GFFFeatureSource.java @@ -105,8 +105,8 @@ public List getCoverageScores(String chr, int start, int end, int zo } @Override - public int estimateFeatureWindowSize() { - return wrappedSource.estimateFeatureWindowSize(); + public int getFeatureWindowSize() { + return wrappedSource.getFeatureWindowSize(); } } diff --git a/src/main/java/org/broad/igv/feature/tribble/IGVFeatureReader.java b/src/main/java/org/broad/igv/feature/tribble/IGVFeatureReader.java index 8449de024a..da793e7fa7 100644 --- a/src/main/java/org/broad/igv/feature/tribble/IGVFeatureReader.java +++ b/src/main/java/org/broad/igv/feature/tribble/IGVFeatureReader.java @@ -26,6 +26,8 @@ package org.broad.igv.feature.tribble; + + import htsjdk.tribble.Feature; import htsjdk.tribble.index.Index; @@ -36,21 +38,21 @@ /** * Interface to replace the Tribble equivalent. Returns plain "Iterator" rather than "CloseableIterator". A wrapper * implementation is provided that handles closing the tribble resource. - *

      + * * NOTE: IGV classes should use this interface, rather than the Tribble FeatureReader. * * @author jrobinso - * Date: 5/8/13 - * Time: 10:55 AM + * Date: 5/8/13 + * Time: 10:55 AM */ public interface IGVFeatureReader { - Iterator query(final String chr, final int start, final int end) throws IOException; + public Iterator query(final String chr, final int start, final int end) throws IOException; - Iterator iterator() throws IOException; + public Iterator iterator() throws IOException; - List getSequenceNames(); + public List getSequenceNames(); - Object getHeader(); + public Object getHeader(); } diff --git a/src/main/java/org/broad/igv/feature/tribble/MUTCodec.java b/src/main/java/org/broad/igv/feature/tribble/MUTCodec.java index 388ec35999..27396107cb 100644 --- a/src/main/java/org/broad/igv/feature/tribble/MUTCodec.java +++ b/src/main/java/org/broad/igv/feature/tribble/MUTCodec.java @@ -180,7 +180,7 @@ public Mutation decode(String line) { int start; try { - start = Integer.parseInt(tokens[startColumn].trim()) - 1; + start = Integer.parseInt(tokens[startColumn].trim()); } catch (NumberFormatException e) { errorCount++; if (errorCount > 100) { @@ -204,6 +204,14 @@ public Mutation decode(String line) { } } + + // MAF files use the 1-based inclusive convention for coordinates. The convention is not + // specified for MUT files, and it appears both conventions have been used. We can detect + // the convention used for single base mutations by testing start == end. + if (isMAF || (start == end) || coords == TrackProperties.BaseCoord.ONE) { + start--; + } + String sampleId = "Unknown"; if(sampleColumn >= 0) { sampleId = tokens[sampleColumn].trim(); diff --git a/src/main/java/org/broad/igv/feature/tribble/reader/BamIndex.java b/src/main/java/org/broad/igv/feature/tribble/reader/BamIndex.java deleted file mode 100644 index bf32fbbf5a..0000000000 --- a/src/main/java/org/broad/igv/feature/tribble/reader/BamIndex.java +++ /dev/null @@ -1,310 +0,0 @@ -package org.broad.igv.feature.tribble.reader; - -import htsjdk.samtools.util.BlockCompressedInputStream; -import org.broad.igv.feature.genome.Genome; -import org.broad.igv.util.LittleEndianInputStream; - -import java.io.ByteArrayInputStream; -import java.io.ByteArrayOutputStream; -import java.io.EOFException; -import java.io.IOException; -import java.nio.ByteBuffer; -import java.util.*; -import java.util.zip.GZIPInputStream; - -public class BamIndex { - - // Represents a BAM index. - // Code is based heavily on bam.js, part of the Dalliance Genome Explorer, (c) Thomas Down 2006-2001. - - int BAI_MAGIC = 21578050; - int TABIX_MAGIC = 21578324; - - int MB = 1000000; - String[] seqNames; - Map sequenceIndexMap; - private boolean tabix; - private long firstBlockPosition; - private long lastBlockPosition; - - private Map indeces; - - - public void parse(byte[] arrayBuffer, Genome genome) throws IOException { - - // TODO -- handle big endian? - LittleEndianInputStream lis; - - // Some indexs are bgzipped, specifically tabix, and csi. Bam (bai) are not. Tribble is usually not. - // Check first 2 bytes of file for gzip magic number, and inflate if neccessary - if (arrayBuffer[0] == 31 && arrayBuffer[1] == -117) { // gzipped - lis = new LittleEndianInputStream(new BlockCompressedInputStream(new ByteArrayInputStream(arrayBuffer))); - } else { - lis = new LittleEndianInputStream(new ByteArrayInputStream(arrayBuffer)); - } - - - int magic = lis.readInt(); - Map sequenceIndexMap = new HashMap<>(); - - - if (!(magic == BAI_MAGIC || magic == TABIX_MAGIC)) { - throw new RuntimeException("Unrecognized magic value: " + magic); - } - - long blockMin = Long.MAX_VALUE; - long blockMax = 0l; - String[] seqNames; - - this.tabix = magic == TABIX_MAGIC; - - int nref = lis.readInt(); - seqNames = new String[nref]; - - if (tabix) { - // Tabix header parameters aren't used, but they must be read to advance the pointer - int format = lis.readInt(); - int col_seq = lis.readInt(); - int col_beg = lis.readInt(); - int col_end = lis.readInt(); - int meta = lis.readInt(); - int skip = lis.readInt(); - int l_nm = lis.readInt(); - - for (int i = 0; i < nref; i++) { - - String seq_name = lis.readString(); - // Translate to "official" chr name. - if (genome != null) { - seq_name = genome.getCanonicalChrName(seq_name); - } - sequenceIndexMap.put(seq_name, i); - seqNames[i] = seq_name; - } - } - - this.firstBlockPosition = blockMin; - this.lastBlockPosition = blockMax; - this.seqNames = seqNames; - this.sequenceIndexMap = sequenceIndexMap; - this.indeces = new HashMap<>(); - - // Loop through sequences - for (int ref = 0; ref < nref; ref++) { - - int nbin = lis.readInt(); - Map> binIndex = new HashMap<>(); - List linearIndex = new ArrayList<>(); - - for (int b = 0; b < nbin; b++) { - int binNumber = lis.readInt(); - if (binNumber == 37450) { - int nchnk = lis.readInt(); - // This is a psuedo bin, not used but we have to consume the bytes - VPointer cs = new VPointer(lis.readLong()); // unmapped beg - VPointer ce = new VPointer(lis.readLong()); // unmapped end - long n_maped = lis.readLong(); - long nUnmapped = lis.readLong(); - } else { - - binIndex.put(binNumber, new ArrayList<>()); - int nchnk = lis.readInt(); // # of chunks for this bin - - for (int i = 0; i < nchnk; i++) { - VPointer cs = new VPointer(lis.readLong()); //chunk_beg - VPointer ce = new VPointer(lis.readLong()); //chunk_end - - if (cs.block < blockMin) { - blockMin = cs.block; // Block containing first alignment - } - if (ce.block > blockMax) { - blockMax = ce.block; - } - binIndex.get(binNumber).add(new Chunk(cs, ce)); - - } - } - } - - List binNumberList = new ArrayList<>(binIndex.keySet()); - Collections.sort(binNumberList); - - int nintv = lis.readInt(); - for (int i = 0; i < nintv; i++) { - VPointer cs = new VPointer(lis.readLong()); - linearIndex.add(cs); // Might be null - } - - if (nbin > 0) { - this.indeces.put(ref, new SeqIndex(binIndex, linearIndex)); - } - } - - - this.firstBlockPosition = blockMin; - this.lastBlockPosition = blockMax; - this.sequenceIndexMap = sequenceIndexMap; - this.tabix = tabix; - - } - - - public Set sequenceNames() { - return this.sequenceIndexMap.keySet(); - } - - /** - * Fetch blocks for a particular genomic range. This method is public so it can be unit-tested. - * - * @param refId the sequence dictionary index of the chromosome - * @param min genomic start position - * @param max genomic end position - */ - List chunksForRange(int refId, int min, int max) { - - SeqIndex ba = this.indeces.get(refId); - - if (ba == null) { - return Collections.EMPTY_LIST; - } else { - List overlappingBins = reg2bins(min, max); // List of bin ranges that overlap min, max - List chunks = new ArrayList<>(); - - // Find chunks in overlapping bins. Leaf bins (< 4681) are not pruned - for (int[] binRange : overlappingBins) { - for (int bin = binRange[0]; bin <= binRange[1]; bin++) { - if (ba.binIndex.get(bin) != null) { - List binChunks = ba.binIndex.get(bin); - chunks.addAll(binChunks); - } - } - } - - - // Use the linear index to find minimum file position of chunks that could contain alignments in the region - int nintv = ba.linearIndex.size(); - VPointer lowest = null; - int minLin = Math.min(min >> 14, nintv - 1); - int maxLin = Math.min(max >> 14, nintv - 1); - for (int i = minLin; i <= maxLin; ++i) { - VPointer vp = ba.linearIndex.get(i); - if (vp != null) { - // todo -- I think, but am not sure, that the values in the linear index have to be in increasing order. So the first non-null should be minimum - if (lowest == null || vp.isLessThan(lowest)) { - lowest = vp; - } - } - } - return optimizeChunks(chunks, lowest); - } - } - - - List optimizeChunks(List chunks, VPointer lowest) { - - List mergedChunks = new ArrayList<>(); - Chunk lastChunk = null; - - if (chunks.isEmpty()) return chunks; - - chunks.sort((c0, c1) -> { - long dif = c0.left.block - c1.left.block; - if (dif != 0) { - return (int) dif; - } else { - return c0.left.offset - c1.left.offset; - } - }); - - for (Chunk chunk : chunks) { - - if (lowest == null || chunk.right.isGreaterThan(lowest)) { - if (lastChunk == null) { - mergedChunks.add(chunk); - lastChunk = chunk; - } else { - if (canMerge(lastChunk, chunk)) { - if (chunk.right.isGreaterThan(lastChunk.right)) { - lastChunk.right = chunk.right; - } - } else { - mergedChunks.add(chunk); - lastChunk = chunk; - } - } - } else { - //console.log(`skipping chunk ${chunk.minv.block} - ${chunk.maxv.block}`) - } - } - - return mergedChunks; - } - - // -// /** -// * Merge 2 blocks if the gap between them is < 1kb and the total resulting size < 100mb -// * -// * @param chunk1 -// * @param chunk2 -// * @returns {boolean|boolean} -// */ - boolean canMerge(Chunk chunk1, Chunk chunk2) { - long gap = chunk2.left.block - chunk1.right.block; - long total = chunk2.right.block - chunk1.left.block; - return gap < 30000 && total < 10 * MB; - } - - - /** - * Calculate the list of bins that overlap with region [beg, end] - * - * @return List of int arrays, each defining a bin range - */ - List reg2bins(int beg, int end) { - List list = new ArrayList<>(); - if (end >= 1 << 29) end = 1 << 29; - --end; - list.add(new int[]{0, 0}); - list.add(new int[]{1 + (beg >> 26), 1 + (end >> 26)}); - list.add(new int[]{9 + (beg >> 23), 9 + (end >> 23)}); - list.add(new int[]{73 + (beg >> 20), 73 + (end >> 20)}); - list.add(new int[]{585 + (beg >> 17), 585 + (end >> 17)}); - list.add(new int[]{4681 + (beg >> 14), 4681 + (end >> 14)}); - return list; - } - - static class Block { - Chunk minv; - Chunk maxv; - int bin; - - public Block(Chunk minv, Chunk maxv, int bin) { - this.minv = minv; - this.maxv = maxv; - this.bin = bin; - } - } - - static class Chunk { - - VPointer left; - VPointer right; - - public Chunk(VPointer left, VPointer right) { - this.left = left; - this.right = right; - } - } - - static class SeqIndex { - Map> binIndex; - List linearIndex; - - public SeqIndex(Map> binIndex, List linearIndex) { - this.binIndex = binIndex; - this.linearIndex = linearIndex; - } - } - -} - diff --git a/src/main/java/org/broad/igv/feature/tribble/reader/CSIIndex.java b/src/main/java/org/broad/igv/feature/tribble/reader/CSIIndex.java deleted file mode 100644 index b1f6b2ffb3..0000000000 --- a/src/main/java/org/broad/igv/feature/tribble/reader/CSIIndex.java +++ /dev/null @@ -1,328 +0,0 @@ -package org.broad.igv.feature.tribble.reader; - -import htsjdk.samtools.util.BlockCompressedInputStream; -import org.broad.igv.feature.genome.Genome; -import org.broad.igv.util.LittleEndianInputStream; - -import java.io.ByteArrayInputStream; -import java.io.IOException; -import java.util.*; - -public class CSIIndex { - - // Represents a BAM index. - // Code is based heavily on bam.js, part of the Dalliance Genome Explorer, (c) Thomas Down 2006-2001. - - static final int CSI1_MAGIC = 21582659; // CSI\1 - static final int CSI2_MAGIC = 38359875; // CSI\2 - - int MB = 1000000; - String[] seqNames; - Map sequenceIndexMap; - private boolean tabix; - private long firstBlockPosition; - private long lastBlockPosition; - - private Map indeces; - private int minShift; - private int depth; - private int maxBinNumber; - - - public void parse(byte[] arrayBuffer, Genome genome) throws IOException { - - // TODO -- handle big endian? - LittleEndianInputStream lis; - - // Some indexs are bgzipped, specifically tabix, and csi. Bam (bai) are not. Tribble is usually not. - // Check first 2 bytes of file for gzip magic number, and inflate if neccessary - if (arrayBuffer[0] == 31 && arrayBuffer[1] == -117) { // gzipped - lis = new LittleEndianInputStream(new BlockCompressedInputStream(new ByteArrayInputStream(arrayBuffer))); - } else { - lis = new LittleEndianInputStream(new ByteArrayInputStream(arrayBuffer)); - } - - int magic = lis.readInt(); - - if (magic != CSI1_MAGIC) { - if (magic == CSI2_MAGIC) { - throw new RuntimeException("CSI version 2 is not supported. Please enter an issue at https://github.com/igvteam/igv.js"); - } else { - throw new RuntimeException("Not a CSI index"); - } - } - - //const indices = [] - long blockMin = Long.MAX_VALUE; - long blockMax = 0l; - - Map sequenceIndexMap = new HashMap<>(); - - this.minShift = lis.readInt(); - this.depth = lis.readInt(); - int lAux = lis.readInt(); - - this.maxBinNumber = (((1 << (this.depth + 1) * 3) - 1) / 7) + 1; - List seqNames = new ArrayList<>(); - if (lAux >= 28) { - // Tabix header parameters aren't used, but they must be read to advance the pointer - int format = lis.readInt(); - int col_seq = lis.readInt(); - int col_beg = lis.readInt(); - int col_end = lis.readInt(); - int meta = lis.readInt(); - int skip = lis.readInt(); - int l_nm = lis.readInt(); - - int i = 0; - int nameCharCount = 0; - while (nameCharCount < l_nm) { - String seq_name = lis.readString(); - nameCharCount += seq_name.length() + 1; - // Translate to "official" chr name. - if (genome != null) { - seq_name = genome.getCanonicalChrName(seq_name); - } - sequenceIndexMap.put(seq_name, i); - seqNames.add(seq_name); - i++; - } - } - - this.indeces = new HashMap<>(); - - // Loop through sequence - int nref = lis.readInt(); - for (int ref = 0; ref < nref; ref++) { - - int nbin = lis.readInt(); - Map> binIndex = new HashMap<>(); - Map linearOffset = new HashMap<>(); - - for (int b = 0; b < nbin; b++) { - - int binNumber = lis.readInt(); - linearOffset.put(binNumber, new VPointer(lis.readLong())); - - if (binNumber > this.maxBinNumber) { - // This is a psuedo bin, not used but we have to consume the bytes - int nchnk = lis.readInt(); - VPointer cs = new VPointer(lis.readLong()); // unmapped beg - VPointer ce = new VPointer(lis.readLong()); // unmapped end - long n_maped = lis.readLong(); - long nUnmapped = lis.readLong(); - } else { - - binIndex.put(binNumber, new ArrayList<>()); - int nchnk = lis.readInt(); // # of chunks for this bin - - for (int i = 0; i < nchnk; i++) { - VPointer cs = new VPointer(lis.readLong()); //chunk_beg - VPointer ce = new VPointer(lis.readLong()); //chunk_end - - if (cs.block < blockMin) { - blockMin = cs.block; // Block containing first alignment - } - if (ce.block > blockMax) { - blockMax = ce.block; - } - binIndex.get(binNumber).add(new Chunk(cs, ce)); - - } - } - } - - List binNumberList = new ArrayList<>(binIndex.keySet()); - Collections.sort(binNumberList); - - if (nbin > 0) { - this.indeces.put(ref, new SeqIndex(binIndex, linearOffset)); - } - } - - - this.firstBlockPosition = blockMin; - this.lastBlockPosition = blockMax; - this.seqNames = seqNames.toArray(new String[seqNames.size()]); - this.sequenceIndexMap = sequenceIndexMap; - - } - - - public Set sequenceNames() { - return this.sequenceIndexMap.keySet(); - } - - /** - * Fetch blocks for a particular genomic range. This method is public so it can be unit-tested. - * - * @param refId the sequence dictionary index of the chromosome - * @param min genomic start position - * @param max genomic end position - */ - List chunksForRange(int refId, int min, int max) { - - SeqIndex ba = this.indeces.get(refId); - - if (ba == null) { - return Collections.EMPTY_LIST; - } else { - List overlappingBins = reg2bins(min, max); // List of bin ranges that overlap min, max - if (overlappingBins.size() == 0) return Collections.EMPTY_LIST; - - List chunks = new ArrayList<>(); - - // Find chunks in overlapping bins. Leaf bins (< 4681) are not pruned - for (int[] binRange : overlappingBins) { - for (int bin = binRange[0]; bin <= binRange[1]; bin++) { - if (ba.binIndex.get(bin) != null) { - List binChunks = ba.binIndex.get(bin); - chunks.addAll(binChunks); - } - } - } - - - // Use the linear index to find minimum file position of chunks that could contain alignments in the region - - VPointer lowest = ba.linearOffsets.get(overlappingBins.get(0)); - - return optimizeChunks(chunks, lowest); - } - } - - - List optimizeChunks(List chunks, VPointer lowest) { - - List mergedChunks = new ArrayList<>(); - Chunk lastChunk = null; - - if (chunks.isEmpty()) return chunks; - - chunks.sort((c0, c1) -> { - long dif = c0.left.block - c1.left.block; - if (dif != 0) { - return (int) dif; - } else { - return c0.left.offset - c1.left.offset; - } - }); - - for (Chunk chunk : chunks) { - - if (lowest == null || chunk.right.isGreaterThan(lowest)) { - if (lastChunk == null) { - mergedChunks.add(chunk); - lastChunk = chunk; - } else { - if (canMerge(lastChunk, chunk)) { - if (chunk.right.isGreaterThan(lastChunk.right)) { - lastChunk.right = chunk.right; - } - } else { - mergedChunks.add(chunk); - lastChunk = chunk; - } - } - } else { - //console.log(`skipping chunk ${chunk.minv.block} - ${chunk.maxv.block}`) - } - } - - return mergedChunks; - } - - // -// /** -// * Merge 2 blocks if the gap between them is < 1kb and the total resulting size < 100mb -// * -// * @param chunk1 -// * @param chunk2 -// * @returns {boolean|boolean} -// */ - boolean canMerge(Chunk chunk1, Chunk chunk2) { - long gap = chunk2.left.block - chunk1.right.block; - long total = chunk2.right.block - chunk1.left.block; - return gap < 30000 && total < 10 * MB; - } - - - /** - * Calculate the list of bins that overlap with region [beg, end] - * reg2bins implementation adapted from GMOD/tabix-js https://github.com/GMOD/tabix-js/blob/master/src/csi.ts - * - * @return List of int arrays, each defining a bin range - */ - List reg2bins(int beg, int end) { - - beg -= 1; // < convert to 1-based closed - if (beg < 1) beg = 1; - if (end > Math.pow(2, 34)) end = (int) Math.pow(2, 34) ;// 17 GiB ought to be enough for anybody - end -= 1; - int l = 0; - int t = 0; - int s = this.minShift + this.depth * 3; - - - List bins = new ArrayList<>(); - for (; l <= this.depth; s -= 3, t += (1 << l * 3), l += 1) { - int b = t + (beg >> s); - int e = t + (end >> s); - if (e - b + bins.size() > this.maxBinNumber) - throw new RuntimeException("" + (beg - end) + "is too large for current binning scheme (shift " + - this.minShift + ", depth " + this.depth + "), try a smaller query or a coarser index binning scheme"); - //for (let i = b; i <= e; i += 1) bins.push(i) - bins.add(new int[]{b, e}); - } - - return bins; - } - - public int getDepth() { - return this.depth; - } - - public int getMinShift() { - return this.minShift; - } - - public int getMaxBinNumber() { - return this.maxBinNumber; - } - - - static class Block { - Chunk minv; - Chunk maxv; - int bin; - - public Block(Chunk minv, Chunk maxv, int bin) { - this.minv = minv; - this.maxv = maxv; - this.bin = bin; - } - } - - static class Chunk { - - VPointer left; - VPointer right; - - public Chunk(VPointer left, VPointer right) { - this.left = left; - this.right = right; - } - } - - static class SeqIndex { - Map> binIndex; - Map linearOffsets; - - public SeqIndex(Map> binIndex, Map linearOffsets) { - this.binIndex = binIndex; - this.linearOffsets = linearOffsets; - } - } - -} - diff --git a/src/main/java/org/broad/igv/feature/tribble/reader/VPointer.java b/src/main/java/org/broad/igv/feature/tribble/reader/VPointer.java deleted file mode 100644 index ea87ee222d..0000000000 --- a/src/main/java/org/broad/igv/feature/tribble/reader/VPointer.java +++ /dev/null @@ -1,29 +0,0 @@ -package org.broad.igv.feature.tribble.reader; - - -public class VPointer { - - private static final int SHIFT_AMOUNT = 16; - private static final int OFFSET_MASK = 0xffff; - private static final long ADDRESS_MASK = 0xFFFFFFFFFFFFL; - - - long block; - int offset; - - public VPointer(long v) { - this.block = (v >> SHIFT_AMOUNT) & ADDRESS_MASK; - this.offset = (int) (v & OFFSET_MASK); - } - - public boolean isLessThan(VPointer vp) { - return this.block < vp.block || - (this.block == vp.block && this.offset < vp.offset); - } - - public boolean isGreaterThan(VPointer vp) { - return this.block > vp.block || - (this.block == vp.block && this.offset > vp.offset); - } - -} \ No newline at end of file diff --git a/src/main/java/org/broad/igv/htsget/HtsgetVariantSource.java b/src/main/java/org/broad/igv/htsget/HtsgetVariantSource.java index 9d4f6170cd..5305fd77ee 100644 --- a/src/main/java/org/broad/igv/htsget/HtsgetVariantSource.java +++ b/src/main/java/org/broad/igv/htsget/HtsgetVariantSource.java @@ -92,7 +92,7 @@ public List getCoverageScores(String chr, int start, int end, int zo } @Override - public int estimateFeatureWindowSize() { + public int getFeatureWindowSize() { return featureWindowSize; } diff --git a/src/main/java/org/broad/igv/session/IGVSessionReader.java b/src/main/java/org/broad/igv/session/IGVSessionReader.java index 6f43ea8dbd..bc450e19c3 100644 --- a/src/main/java/org/broad/igv/session/IGVSessionReader.java +++ b/src/main/java/org/broad/igv/session/IGVSessionReader.java @@ -252,11 +252,9 @@ private void processRootNode(Session session, Node node, HashMap additionalInfor try { GenomeListItem item = GenomeListManager.getInstance().getGenomeListItem(genomeId); if (item != null) { - // Genome specified by know ID (e.g. hg19) genomePath = item.getPath(); GenomeManager.getInstance().loadGenome(item.getPath(), null); } else { - // Not a recognized ID, possible a local path or URL genomePath = genomeId; if (!ParsingUtils.fileExists(genomePath)) { genomePath = getAbsolutePath(genomeId, rootPath); diff --git a/src/main/java/org/broad/igv/tools/motiffinder/MotifFinderPlugin.java b/src/main/java/org/broad/igv/tools/motiffinder/MotifFinderPlugin.java index 431ec107f5..ee5ddc0bb5 100644 --- a/src/main/java/org/broad/igv/tools/motiffinder/MotifFinderPlugin.java +++ b/src/main/java/org/broad/igv/tools/motiffinder/MotifFinderPlugin.java @@ -25,7 +25,10 @@ package org.broad.igv.tools.motiffinder; +import org.broad.igv.feature.CachingFeatureSource; import org.broad.igv.feature.Strand; +import org.broad.igv.feature.genome.GenomeManager; +import org.broad.igv.track.FeatureTrack; import org.broad.igv.track.MotifTrack; import org.broad.igv.track.Track; import org.broad.igv.ui.IGV; @@ -34,6 +37,8 @@ import javax.swing.*; import java.awt.*; +import java.awt.event.ActionEvent; +import java.awt.event.ActionListener; import java.util.ArrayList; import java.util.List; diff --git a/src/main/java/org/broad/igv/tools/motiffinder/MotifFinderSource.java b/src/main/java/org/broad/igv/tools/motiffinder/MotifFinderSource.java index 2c1724afe6..382d7b63ba 100644 --- a/src/main/java/org/broad/igv/tools/motiffinder/MotifFinderSource.java +++ b/src/main/java/org/broad/igv/tools/motiffinder/MotifFinderSource.java @@ -71,9 +71,6 @@ public class MotifFinderSource implements FeatureSource, Persistable { private Strand strand; - /** - * Empty constructor for unmarshalling session - */ public MotifFinderSource() { this.genome = GenomeManager.getInstance().getCurrentGenome(); } @@ -170,7 +167,7 @@ public List getCoverageScores(String chr, int start, int end, int zo } @Override - public int estimateFeatureWindowSize() { + public int getFeatureWindowSize() { return this.featureWindowSize; } diff --git a/src/main/java/org/broad/igv/track/FeatureCollectionSource.java b/src/main/java/org/broad/igv/track/FeatureCollectionSource.java index 7a7612f5d8..be7674e720 100644 --- a/src/main/java/org/broad/igv/track/FeatureCollectionSource.java +++ b/src/main/java/org/broad/igv/track/FeatureCollectionSource.java @@ -38,7 +38,6 @@ import org.broad.igv.util.collections.CollUtils; import htsjdk.tribble.Feature; -import java.io.IOException; import java.util.*; /** @@ -66,6 +65,7 @@ public FeatureCollectionSource(Iterable allFeatures, Genome g initFeatures(allFeatures); coverageData = new CoverageDataSource(genome); coverageData.computeGenomeCoverage(); + sampleGenomeFeatures(); } public List getCoverageScores(String chr, int startLocation, int endLocation, int zoom) { @@ -81,22 +81,26 @@ public List getCoverageScores(String chr, int startLocation, int end * @return */ public Iterator getFeatures(String chr, int start, int end) { + return getFeatureList(chr, start, end).iterator(); + } + + public List getFeatureList(String chr, int start, int end) { List features = featureMap.get(chr); if (features == null) { - return Collections.emptyList().iterator(); + return Collections.emptyList(); } List filteredFeatures = CollUtils.filter(features, FeatureUtils.getOverlapPredicate(chr, start, end)); - return filteredFeatures.iterator(); + return filteredFeatures; } @Override - public List getAllFeatures() throws IOException { - List allFeatures = new ArrayList<>(); - for(List f : featureMap.values()) { - allFeatures.addAll(f); - } - return allFeatures; + public boolean isLoaded(ReferenceFrame frame) { + return true; // All features are loaded by definition + } + + public List getFeatures(String chr) { + return featureMap.get(chr); } private void initFeatures(Iterable allFeatures) { @@ -115,6 +119,10 @@ private void initFeatures(Iterable allFeatures) { for (List featureList : featureMap.values()) { FeatureUtils.sortFeatureList(featureList); } + + if (featureMap.size() < 100) { + sampleGenomeFeatures(); + } } @@ -148,7 +156,7 @@ protected void sampleGenomeFeatures() { int sampleLength = (int) ((double) genome.getNominalLength() / (1000 * 700)); int lastFeaturePosition = -1; for (String chr : genome.getLongChromosomeNames()) { - List features =featureMap.get(chr); + List features = getFeatures(chr); if (features != null) { long offset = genome.getCumulativeOffset(chr); for (Feature feature : features) { diff --git a/src/main/java/org/broad/igv/track/FeatureSource.java b/src/main/java/org/broad/igv/track/FeatureSource.java index f7af7ce622..b001557f65 100644 --- a/src/main/java/org/broad/igv/track/FeatureSource.java +++ b/src/main/java/org/broad/igv/track/FeatureSource.java @@ -27,6 +27,7 @@ import htsjdk.tribble.Feature; import org.broad.igv.feature.LocusScore; +import org.broad.igv.ui.panel.ReferenceFrame; import java.io.IOException; import java.util.Iterator; @@ -40,6 +41,10 @@ */ public interface FeatureSource { + default boolean isLoaded(ReferenceFrame frame) { + return false; + } + /** * Return an iterator over all features that overlap the interval. The coordinates are in the "UCSC" convention, * that is first base is zero and the interval is not inclusive of the end position, i.e. an interval @@ -53,18 +58,6 @@ public interface FeatureSource { Iterator getFeatures(String chr, int start, int end) throws IOException; - /** - * Return all features for the entire genome as a list. Not supported by all feature sources. - * - * @return - * @throws IOException - */ - default List getAllFeatures() throws IOException { - return null; - } - - - /** * Return a list of coverage values spanning the given interval. This can be null if coverage is not known * or relevant. @@ -83,9 +76,12 @@ default List getAllFeatures() throws IOException { * rendering features. When zoomed out beyond this interval coverage is shown, if available, or a message to * zoom in. * + * TODO: It seems slightly odd to have this property here, but on the other hand FeatureSource objects have + * TODO: access to the information required to estimate this value. Still, consider removing this from the interface. + * * @return the feature window threshold in base-pairs */ - default int estimateFeatureWindowSize() { + default int getFeatureWindowSize() { return -1; } diff --git a/src/main/java/org/broad/igv/track/FeatureTrack.java b/src/main/java/org/broad/igv/track/FeatureTrack.java index d51da2788e..35865fbfa8 100644 --- a/src/main/java/org/broad/igv/track/FeatureTrack.java +++ b/src/main/java/org/broad/igv/track/FeatureTrack.java @@ -25,11 +25,8 @@ package org.broad.igv.track; -import htsjdk.samtools.util.Locatable; import htsjdk.tribble.Feature; import htsjdk.tribble.TribbleException; -import org.broad.igv.feature.genome.Genome; -import org.broad.igv.feature.genome.GenomeManager; import org.broad.igv.logging.*; import org.broad.igv.Globals; import org.broad.igv.event.DataLoadedEvent; @@ -37,6 +34,8 @@ import org.broad.igv.event.IGVEventObserver; import org.broad.igv.feature.*; import org.broad.igv.feature.Range; +import org.broad.igv.feature.genome.Genome; +import org.broad.igv.feature.genome.GenomeManager; import org.broad.igv.prefs.Constants; import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.renderer.*; @@ -119,7 +118,6 @@ public class FeatureTrack extends AbstractTrack implements IGVEventObserver { private boolean groupByStrand = false; private String labelField; - private ArrayList wgFeatures; public FeatureTrack() { @@ -197,7 +195,7 @@ protected void init(ResourceLocator locator, FeatureSource source) { if (vizWindow != null) { visibilityWindow = vizWindow; } else { - int sourceFeatureWindowSize = source.estimateFeatureWindowSize(); + int sourceFeatureWindowSize = source.getFeatureWindowSize(); int defVisibilityWindow = PreferencesManager.getPreferences().getAsInt(Constants.DEFAULT_VISIBILITY_WINDOW); if (sourceFeatureWindowSize > 0 && defVisibilityWindow > 0) { // Only apply a default if the feature source supports visibility window. visibilityWindow = defVisibilityWindow * 1000; @@ -681,58 +679,69 @@ public boolean isReadyToPaint(ReferenceFrame frame) { } } + public void load(ReferenceFrame frame) { + loadFeatures(frame.getChrName(), (int) frame.getOrigin(), (int) frame.getEnd(), frame.getName()); + } + /** - * Loads and segregates features for the reference frame into rows such that they do not overlap. + * Loads and segregates features into rows such that they do not overlap. + * + * @param chr + * @param start + * @param end */ - public void load(ReferenceFrame frame) { + protected void loadFeatures(final String chr, final int start, final int end, final String frame) { if (source == null) { return; } - final String chr = frame.getChrName(); - final int start = (int) frame.getOrigin(); - final int end = (int) frame.getEnd(); - try { - Iterator iter; int expandedStart; int expandedEnd; - - if(Globals.CHR_ALL.equals(chr)) { - iter = sampleGenomeFeatures().iterator(); - expandedStart = 0; - expandedEnd = Integer.MAX_VALUE; - } else { - int vw = getVisibilityWindow(); - if (vw > 0) { - int delta = (end - start) / 2; - expandedStart = start < 0 ? start : start - delta; - expandedEnd = end + delta; - if (expandedEnd < 0) { - expandedEnd = Integer.MAX_VALUE; // assumed integer overflow - } - } else { - expandedStart = 0; - expandedEnd = Integer.MAX_VALUE; + int vw = getVisibilityWindow(); + if (vw > 0) { + int delta = (end - start) / 2; + expandedStart = start - delta; + expandedEnd = end + delta; + if (expandedEnd < 0) { + expandedEnd = Integer.MAX_VALUE; // overflow } - iter = source.getFeatures(chr, expandedStart, expandedEnd); + } else { + expandedStart = 0; + expandedEnd = Integer.MAX_VALUE; + } + + + //Make sure we are only querying within the chromosome we allow for somewhat pathological cases of start + //being negative and end being outside, but only if directly queried. Our expansion should not + //set start < 0 or end > chromosomeLength + if (start >= 0) { + expandedStart = Math.max(0, expandedStart); + } + + Genome genome = GenomeManager.getInstance().getCurrentGenome(); + if (genome != null) { + Chromosome c = genome.getChromosome(chr); + if (c != null && end < c.getLength()) expandedEnd = Math.min(c.getLength(), expandedEnd); } + Iterator iter = source.getFeatures(chr, expandedStart, expandedEnd); + if (iter == null) { PackedFeatures pf = new PackedFeatures(chr, expandedStart, expandedEnd); - packedFeaturesMap.put(frame.getName(), pf); + packedFeaturesMap.put(frame, pf); } else { PackedFeatures pf = new PackedFeatures(chr, expandedStart, expandedEnd, iter, this.getDisplayMode(), groupByStrand); - packedFeaturesMap.put(frame.getName(), pf); + packedFeaturesMap.put(frame, pf); //log.warn("Loaded " + chr + " " + expandedStart + "-" + expandedEnd); } } catch (Exception e) { // Mark the interval with an empty feature list to prevent an endless loop of load attempts. PackedFeatures pf = new PackedFeatures(chr, start, end); - packedFeaturesMap.put(frame.getName(), pf); + packedFeaturesMap.put(frame, pf); String msg = "Error loading features for interval: " + chr + ":" + start + "-" + end + "
      " + e.toString(); MessageUtils.showMessage(msg); log.error(msg, e); @@ -746,6 +755,7 @@ public void render(RenderContext context, Rectangle rect) { renderRect.y = renderRect.y + margin; renderRect.height -= margin; + showFeatures = isShowFeatures(context.getReferenceFrame()); if (showFeatures) { if (lastFeatureMode != null) { @@ -775,13 +785,13 @@ public void render(RenderContext context, Rectangle rect) { protected boolean isShowFeatures(ReferenceFrame frame) { -// if (frame.getChrName().equals(Globals.CHR_ALL) && coverageRenderer != null) { -// return false; -// } else { - double windowSize = frame.getEnd() - frame.getOrigin(); - int vw = getVisibilityWindow(); - return (vw <= 0 || windowSize <= vw); -// } + if (frame.getChrName().equals(Globals.CHR_ALL)) { + return false; + } else { + double windowSize = frame.getEnd() - frame.getOrigin(); + int vw = getVisibilityWindow(); + return (vw <= 0 || windowSize <= vw); + } } protected void renderCoverage(RenderContext context, Rectangle inputRect) { @@ -1014,40 +1024,6 @@ public List getVisibleFeatures(ReferenceFrame frame) { } } - private List sampleGenomeFeatures() throws IOException { - - if (wgFeatures == null) { - - Genome genome = GenomeManager.getInstance().getCurrentGenome(); - List allFeatures = this.source.getAllFeatures(); - if (allFeatures == null) return Collections.EMPTY_LIST; - - wgFeatures = new ArrayList(allFeatures.size()); - - wgFeatures.sort(Comparator.comparingInt(Locatable::getStart)); - - Set chrNames = new HashSet<>(genome.getLongChromosomeNames()); - - for (Feature feature : allFeatures) { - - String chr = genome.getCanonicalChrName(feature.getChr()); - if (!chrNames.contains(chr)) continue; - - long offset = genome.getCumulativeOffset(chr); - - if (feature instanceof IGVFeature) { - IGVFeature f = (IGVFeature) feature; - int genStart = (int) ((offset + f.getStart()) / 1000); - int genEnd = (int) ((offset + f.getEnd()) / 1000); - - WGFeature f2 = new WGFeature(f, genStart, genEnd); - - wgFeatures.add(f2); - } - } - } - return wgFeatures; - } public void setTrackLine(String trackLine) { this.trackLine = trackLine; diff --git a/src/main/java/org/broad/igv/track/MotifTrack.java b/src/main/java/org/broad/igv/track/MotifTrack.java index dc60bfc335..e8d2724354 100644 --- a/src/main/java/org/broad/igv/track/MotifTrack.java +++ b/src/main/java/org/broad/igv/track/MotifTrack.java @@ -1,5 +1,6 @@ package org.broad.igv.track; +import org.broad.igv.feature.CachingFeatureSource; import org.broad.igv.feature.Strand; import org.broad.igv.feature.genome.GenomeManager; import org.broad.igv.tools.motiffinder.MotifFinderSource; @@ -25,7 +26,10 @@ public MotifTrack(String name, String pattern, Strand strand) { } private void init() { - super.init(null, new MotifFinderSource(pattern, strand, GenomeManager.getInstance().getCurrentGenome())); + + MotifFinderSource src = new MotifFinderSource(pattern, strand, GenomeManager.getInstance().getCurrentGenome()); + CachingFeatureSource source = new CachingFeatureSource(src); + super.init(null, source); setSortable(false); } diff --git a/src/main/java/org/broad/igv/track/MutationTrack.java b/src/main/java/org/broad/igv/track/MutationTrack.java index 45c9e9dd36..7c1d971e42 100644 --- a/src/main/java/org/broad/igv/track/MutationTrack.java +++ b/src/main/java/org/broad/igv/track/MutationTrack.java @@ -71,6 +71,19 @@ public void overlay(RenderContext context, Rectangle rect) { } + /** + * Return a string for popup text. + * + * @param chr + * @param position in genomic coordinates + * @param mouseX + * @return + */ + @Override + public String getValueStringAt(String chr, double position, int mouseX, int mouseY, ReferenceFrame frame) { + return super.getValueStringAt(chr, position, mouseX, mouseY, frame); //To change body of overridden methods use File | Settings | File Templates. + } + @Override public boolean handleDataClick(TrackClickEvent te) { super.openTooltipWindow(te); @@ -78,4 +91,69 @@ public boolean handleDataClick(TrackClickEvent te) { } + private String getOMAText(Mutation mut, String url) { + try { + String omaWebService = url + "&frm=txt&fts=all"; + + String result = HttpUtils.getInstance().getContentsAsString(HttpUtils.createURL(omaWebService)); + + BufferedReader br = new BufferedReader(new StringReader(result)); + String[] headers = br.readLine().split("\t"); + String[] values = br.readLine().split("\t"); + + StringBuffer buf = new StringBuffer(); + + buf.append(""); + buf.append(""); + buf.append(""); + + int n = Math.min(headers.length, values.length); + for (int i = 0; i < n; i++) { + final String header = headers[i].trim(); + final String value = values[i].trim(); + + if (header.length() == 0 || value.length() == 0) continue; + + buf.append(""); + buf.append(""); + } + buf.append("
      Data fromMutation Assessor
      Type" + mut.getMutationType() + "
      "); + buf.append(header); + buf.append(""); + + if (header.equals("MSA") || header.equals("PDB")) { + buf.append(""); + buf.append(header); + buf.append(""); + } else if (header.equals("Uniprot")) { + buf.append(""); + buf.append(value); + buf.append(""); + } else if (header.equals("Refseq")) { + buf.append(""); + buf.append(value); + buf.append(""); + } else if (header.toLowerCase().contains("impact")) { + if (value.toLowerCase().equals("high") || value.toLowerCase().equals("medium")) { + String color = value.toLowerCase().equals("high") ? "red" : "#0033FF"; + buf.append("
      "); + buf.append(value); + buf.append("
      "); + } else { + buf.append(value); + } + } else { + buf.append(value); + } + buf.append("

      "); + + return buf.toString(); + } catch (IOException e) { + log.error("Error accessing OMA ", e); + return null; + } + } + } diff --git a/src/main/java/org/broad/igv/track/TribbleFeatureSource.java b/src/main/java/org/broad/igv/track/TribbleFeatureSource.java index f05872505a..f56891f9b4 100644 --- a/src/main/java/org/broad/igv/track/TribbleFeatureSource.java +++ b/src/main/java/org/broad/igv/track/TribbleFeatureSource.java @@ -118,7 +118,6 @@ public static TribbleFeatureSource getFeatureSource(ResourceLocator locator, Gen } } - indexExists = false; String path = locator.getPath(); if (FileUtils.isRemote(path)) { path = HttpUtils.createURL(path).toExternalForm(); @@ -187,7 +186,7 @@ public Class getFeatureClass() { return featureClass; } - public int estimateFeatureWindowSize() { + public int getFeatureWindowSize() { if (featureWindowSize == null) { this.featureWindowSize = estimateFeatureWindowSize(this.wrappedReader); } @@ -233,17 +232,6 @@ public Iterator getFeatures(String chr, int start, int end) throws IOEx return reader.query(seqName, start, end); } - public List getAllFeatures() throws IOException { - - List allFeatures = new ArrayList<>(); - Iterator iter = reader.iterator(); - while(iter.hasNext()) { - allFeatures.add(iter.next()); - } - return allFeatures; - } - - /** * Return coverage values overlapping the query interval. At this time Tribble sources do not provide * coverage values @@ -401,6 +389,11 @@ public boolean isIndexed() { return false; } + @Override + public boolean isLoaded(ReferenceFrame frame) { + return true; + } + @Override public List getCoverageScores(String chr, int start, int end, int zoom) { return coverageData == null ? Collections.emptyList() : diff --git a/src/main/java/org/broad/igv/track/TribbleListFeatureSource.java b/src/main/java/org/broad/igv/track/TribbleListFeatureSource.java index d14e403e9b..20d2816c54 100644 --- a/src/main/java/org/broad/igv/track/TribbleListFeatureSource.java +++ b/src/main/java/org/broad/igv/track/TribbleListFeatureSource.java @@ -138,7 +138,7 @@ public List getCoverageScores(String chr, int start, int end, int zo } @Override - public int estimateFeatureWindowSize() { + public int getFeatureWindowSize() { return windowSize; } diff --git a/src/main/java/org/broad/igv/variant/New/Variant.java b/src/main/java/org/broad/igv/variant/New/Variant.java index cb70e72cf6..06009b28fb 100644 --- a/src/main/java/org/broad/igv/variant/New/Variant.java +++ b/src/main/java/org/broad/igv/variant/New/Variant.java @@ -28,15 +28,12 @@ import htsjdk.tribble.Feature; import org.broad.igv.Globals; -import org.broad.igv.feature.IGVFeature; - -import java.awt.*; /** * Parser for VCF files. */ -public class Variant implements IGVFeature { +public class Variant implements Feature { String chr; int pos; @@ -115,18 +112,4 @@ public String getValueString(int genomicPosition) { } - @Override - public Color getColor() { - return null; - } - - @Override - public float getScore() { - return 0; - } - - @Override - public String getName() { - return null; - } } diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index 05de4685ae..68f316b430 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -295,7 +295,6 @@ SMRT_KINETICS.SHOW_OPTIONS FALSE SCORE_VARIANTS FALSE SAM.AUTOSORT FALSE -SAM.INVERT_SORT FALSE SAM.SHADE_CENTER TRUE SAM.SHOW_REF_SEQ FALSE @@ -315,7 +314,6 @@ SAM.GROUP_ALL FALSE SAM.INVERT_SORT FALSE - SEARCH_ZOOM TRUE SAVE_GOOGLE_CREDENTIALS TRUE MAX_SEQUENCE_RESOLUTION 2 diff --git a/src/test/java/org/broad/igv/feature/tribble/reader/BamIndexTest.java b/src/test/java/org/broad/igv/feature/tribble/reader/BamIndexTest.java deleted file mode 100644 index c20259abd2..0000000000 --- a/src/test/java/org/broad/igv/feature/tribble/reader/BamIndexTest.java +++ /dev/null @@ -1,171 +0,0 @@ -/* - * The MIT License (MIT) - * - * Copyright (c) 2007-2015 Broad Institute - * - * Permission is hereby granted, free of charge, to any person obtaining a copy - * of this software and associated documentation files (the "Software"), to deal - * in the Software without restriction, including without limitation the rights - * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell - * copies of the Software, and to permit persons to whom the Software is - * furnished to do so, subject to the following conditions: - * - * The above copyright notice and this permission notice shall be included in - * all copies or substantial portions of the Software. - * - * - * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR - * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, - * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE - * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER - * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, - * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN - * THE SOFTWARE. - */ - -package org.broad.igv.feature.tribble.reader; - -import org.broad.igv.util.TestUtils; -import org.junit.Test; - -import java.io.FileInputStream; -import java.util.List; - -import static org.junit.Assert.*; - -/** - * @author jrobinso - * Date: 1/23/14 - * Time: 4:24 PM - */ -public class BamIndexTest { - - @Test - public void testParse() throws Exception { - - String path = TestUtils.DATA_DIR + "tabix/refGene.hg19.bed.gz.tbi"; - - FileInputStream fis = new FileInputStream(path); - byte[] bytes = fis.readAllBytes(); - - BamIndex tabixIndex = new BamIndex(); - tabixIndex.parse(bytes, null); - assertNotNull(tabixIndex.sequenceIndexMap); - - - } - - @Test - public void testTBIIndex() throws Exception { - - String path = TestUtils.DATA_DIR + "tabix/refGene.hg19.bed.gz.tbi"; - int refID = 26; - int beg = 55369194; - int end = 55369443; - - FileInputStream fis = new FileInputStream(path); - byte[] bytes = fis.readAllBytes(); - - BamIndex tbiIndex = new BamIndex(); - tbiIndex.parse(bytes, null); - - List chunks = tbiIndex.chunksForRange(refID, beg, end); - assertNotNull(tbiIndex.sequenceIndexMap); - assertEquals(chunks.size(), 1); - assertEquals(1640062, chunks.get(0).left.block); - - } - - @Test - public void testCSIIndex() throws Exception { - - String path = TestUtils.DATA_DIR + "tabix/csi-test.vcf.gz.csi"; - int refID = 0; - int beg = 1226000; - int end = 1227000; - - FileInputStream fis = new FileInputStream(path); - byte[] bytes = fis.readAllBytes(); - - CSIIndex csiIndex = new CSIIndex(); - csiIndex.parse(bytes, null); - - List chunks = csiIndex.chunksForRange(refID, beg, end); - assertTrue(chunks.size() > 0); - - } - - @Test - public void testCSIQuery() throws Exception { - - String path = TestUtils.DATA_DIR + "tabix/csi-test.vcf.gz.csi"; - int refID = 0; - int beg = 1226000; - int end = 1227000; - - FileInputStream fis = new FileInputStream(path); - byte[] bytes = fis.readAllBytes(); - - CSIIndex csiIndex = new CSIIndex(); - csiIndex.parse(bytes, null); - - List chunks = csiIndex.chunksForRange(refID, beg, end); - assertEquals(chunks.size(), 1); - assertEquals(74500, chunks.get(0).left.block); - assertEquals(61246, chunks.get(0).left.offset); - assertEquals(94804, chunks.get(0).right.block); - } - - - -// -// test("CSI query - vcf", async function () { -// -// const chr = "chr1", -// beg = 1226000, -// end = 1227000; -// -// const reader = new FeatureFileReader({ -// format: "vcf", -// url: require.resolve("./data/tabix/csi-test.vcf.gz"), -// indexURL: require.resolve("./data/tabix/csi-test.vcf.gz.csi") -// }); -// await reader.readHeader(); -// const features = await reader.readFeatures(chr, beg, end); -// assert.ok(features); -// -// // Verify features are in query range. The features immediately before and after query range are returned by design -// const len = features.length; -// assert.ok(len > 0); -// for (let i = 1; i < len - 1; i++) { -// const f = features[i]; -// assert.ok(f.chr === chr && f.end >= beg && f.start <= end); -// } -// }) -// -// test("CSI query - gtf", async function () { -// -// this.timeout(200000); -// -// const chr = "10", -// beg = 400000, -// end = 500000; -// -// const reader = new FeatureFileReader({ -// format: "gff3", -// url: "https://s3.amazonaws.com/igv.org.genomes/hg38/Homo_sapiens.GRCh38.94.chr.gff3.gz", -// indexURL: "https://s3.amazonaws.com/igv.org.genomes/hg38/Homo_sapiens.GRCh38.94.chr.gff3.gz.csi" -// }); -// await reader.readHeader(); -// const features = await reader.readFeatures(chr, beg, end); -// assert.ok(features); -// -// const len = features.length; -// assert.ok(len > 0); -// for (let i = 1; i < len - 1; i++) { -// const f = features[i]; -// assert.ok(f.chr === chr); -// } -// }) - -} diff --git a/src/test/java/org/broad/igv/feature/tribble/reader/CSIIndexTest.java b/src/test/java/org/broad/igv/feature/tribble/reader/CSIIndexTest.java deleted file mode 100644 index 273cb218dc..0000000000 --- a/src/test/java/org/broad/igv/feature/tribble/reader/CSIIndexTest.java +++ /dev/null @@ -1,94 +0,0 @@ -/* - * The MIT License (MIT) - * - * Copyright (c) 2007-2015 Broad Institute - * - * Permission is hereby granted, free of charge, to any person obtaining a copy - * of this software and associated documentation files (the "Software"), to deal - * in the Software without restriction, including without limitation the rights - * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell - * copies of the Software, and to permit persons to whom the Software is - * furnished to do so, subject to the following conditions: - * - * The above copyright notice and this permission notice shall be included in - * all copies or substantial portions of the Software. - * - * - * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR - * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, - * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE - * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER - * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, - * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN - * THE SOFTWARE. - */ - -package org.broad.igv.feature.tribble.reader; - -import org.broad.igv.util.TestUtils; -import org.junit.Before; -import org.junit.Test; - -import java.io.FileInputStream; -import java.io.IOException; - -import static org.junit.Assert.*; - -/** - * @author jrobinso - * Date: 1/23/14 - * Time: 4:24 PM - */ -public class CSIIndexTest { - - CSIIndex csi; - CSIIndex ucsi; - - @Before - public void init() throws IOException { - - - FileInputStream fis = new FileInputStream(TestUtils.DATA_DIR + "tabix/index_test.bam.csi"); - byte[] bytes = fis.readAllBytes(); - csi = new CSIIndex(); - csi.parse(bytes, null); - - - fis = new FileInputStream(TestUtils.DATA_DIR + "tabix/uncompressed_index.bam.csi"); - bytes = fis.readAllBytes(); - ucsi = new CSIIndex(); - ucsi.parse(bytes, null); - - -// bai = new DiskBasedBAMFileIndex(new File(TEST_DATA_DIR, "index_test.bam.bai"), null); -// csi = new htsjdk.samtools.CSIIndex(new File(TEST_DATA_DIR, "index_test.bam.csi"), false, null); -// mcsi = new htsjdk.samtools.CSIIndex(new File(TEST_DATA_DIR, "index_test.bam.csi"), true, null); -// try { -// ucsi = new CSIIndex(Paths.get(TEST_DATA_DIR, "uncompressed_index.bam.csi"), null); -// } catch (IOException e) { -// e.printStackTrace(); -// } -// ubai = new DiskBasedBAMFileIndex(new File(TEST_DATA_DIR, "uncompressed_index.bam.bai"), null); - } - - @Test - public void testGetNumIndexLevels() { - assertEquals(csi.getDepth(), 5); - assertEquals(ucsi.getDepth(), 6); - } - - @Test - public void testGetMinShift() { - assertEquals(csi.getMinShift(), 14); - assertEquals(ucsi.getMinShift(), 12); - } - - @Test - public void testGetMaxBins() { - assertEquals(csi.getMaxBinNumber(), 37450); - assertEquals(ucsi.getMaxBinNumber(), 299594); - } - - - -} diff --git a/test/data/tabix/TSVC.vcf.gz b/test/data/tabix/TSVC.vcf.gz deleted file mode 100755 index 10646d699313d9872785c66822db1286838919b8..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 4833 zcmV<75+3aziwFb&00000{{{d;LjnNA61`e&bJ|Fd{>=UgRrX7A2lBjYBu-mJjB$MD z*bXMyY+YSZGL~&BfLKW+@&5Yjo{@x*aUhPp72D`#UV7$v`swZ=zyE$Soy9jvI*;=3 z)8XK&aOrQq|2{Z59UTqB|2pZ1XXl5#)6;f8&L-(}nNO2N`06-b#OZY6K;Jx0U1wlE zr;A(XI?ki%EOW9vN#kp0x^MeeY!=UA=O&H+Ud4;ay>k_1FmeJT&ysJ>(bs6UieNS;xys`7YlOX=NwQkx z*@NlM8`GUE^7uARn-juX7EvBMk)8U%z|C>{7v;G02jdRo=-TPW%lxj{f3TlnjrIP# zorC=hZOronyNBmb8t*1f0m+}R`|<%%eszN~Bv5__yh_qiKV0FT{kOYi zWw2qgb2SAHLK?mV3Qi~3q)EijpBt;#Ht6OWZXm}8sWSeP;sQ2@d^h}fwhsk{tA#U* z7q|IcU>3iqlo#T67Ef0B^eb-3GLG`B4Bb(aDARK1%6-MCK93tFU6uxsa*$#VV_;$<_PakPy4%}d>==!LCI}rHvJOMrguq# zay82%XBk`dYG8SG`Evm-i{{H&oH^g7Ac5CW9&HYHu?OAWBEL+moJ9t%Qu~%kW->6u zXN?USojJ(yRaP}M4_)WzZ%FSl=Y6zTbpE`X=8O2=`RJr~32+(4&T^f6~a#AKA2|@ zwb^GLD?E?SbGe6eH0IfG5&)*4NOS1X-XmA;wwAy`K0${nupW;86N3B;6OI15IBHC^ zElnQ*b<9Bm%w{*F?ZgoKfO6Sl*>>jmXS&GR7-riJY}+Kk2>HD+-KXAgkB$3OEMN~M zJdJPi-fViiDEefOJ%G^PL!U7k96o5^T-`6DEW^aiJP-496+2(%aXN;CBc#4FpDxC* zhhtMRhSJIIk~AM@tDBqY_whW+CwK4=8%-Cm+Hp4hCk}-(kG_xZmSdZ=gt{69%hZs# zR8~*2Ns`7PB`&ET8e7(lahcFqJIN|vuJZAG@iin)mc=-5yr_HXfK3xYqgzwfpt^`Uv$0ro}wTr#E;#UM^t? zF2m$9hLkrR-@wj~%aj3{8od6Ap>dX(-Ho$ymCzje5)Y8a-7w4j-~Tg7#P|xP1cS_~ z7pvLKS>7cJ*eA$f!F_sX{ob(efDC;Ze~IsH3)9b*l{m~4gcS5~mf5%Q_sMK^9iMDI zEgOw9*y3~r27Ej_IX_!Jhh4b^e+hf1r)w{R*5+&?4iC~eTg~$9pn7*Yy-K6>{ve-6 z%gzlyxVxpk`QzS1kX<-wa{U&Vg%&Q7MIVz3*hiDaL3(tZ%rE2kI=lf3W9K^gwlKLU zSYY}7_OJ0}@BPK;(fVCH&J&HPv5p1gVWDX=S+1&f zRmW-a@Yz)~H=G*Z!M;K|Ut`H_nyi-Nt64PpGM>TS7Zd~WxlLn3!t&b6W&O#gYC%>{ zN5hlD5y~d;zT(^%ONa4lfg181RspsYI|f-Q5(<>$Ydi~4I`NUg5czKmxr=AZqIseA z;p?I04Stzq_M!{}S+Yv&L@f33z-r9FJMfr;-U`MC7}N2wpdGC{ouS8x@H(eMt?5{f z`~N*=T1q)qQW5W9Ia`5|V;JI~nCB4VLW)mZN*G~`ADkqM&!83@uscoQZgrKppAH9qrc>C&OSpB; z&d2YLdi|r})4GEO#o0Jn%$mp!CUnfCQgtJs<=occcZcK4{bqB!i9 zWINZvDyj%gnZF0@tTIbd5EH3(a%;^KUxS1QzSF8TO?=>%VBXF-)-2J)r;@3ib5tau z9)(GW+UZsmNhl!5wNg9rs7OME;!42cclGesJt(6bfZx^Qeboa*+~3vu^Lh=Ec|`8s zr@z*;1qN<~8$(zh(>g}LY#?6o?BnP#pU&;$4kaBXM}!4T1Ooq8#rl+`Z~vPFR3w+? zXbFTgaD+=*F~&&1ICY%6voM&vR1O;7gvHK)?LUY*R(cmd|uFpj<6gLO$osOO|XqjVe8}fbcV!u(}N6XB?-8VaC)`#{u0B;I8 zGM4Yv=>$Hv`(vlOjw2`zZ1xP?hx8e1O`mz5ke=^L@SekW!}Iqo7`k=RZ^7U#7^8*f zqAmNMm0D>tC%PKHr1~1Y4<}vZfQ1JZB}ZHMw{W~CCqQ$ULIZGg^SVC@8bECeLlj0CA^S$ePN8nlbH|?SD>u! 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T G 50.09 . 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G C 54.3 . 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CCT C 49.48 . AC=6;AF=0.107;AN=56;BaseQRankSum=0.900;DP=65;FS=0.000;HRun=0;HaplotypeScore=12.2055;InbreedingCoeff=-0.1167;MQ=36.09;MQ0=0;MQRankSum=1.148;QD=3.81;ReadPosRankSum=0.124;EFF=DOWNSTREAM(MODIFIER||||85|FAM138A|protein_coding|CODING|ENST00000417324|),DOWNSTREAM(MODIFIER|||||FAM138A|processed_transcript|CODING|ENST00000461467|),DOWNSTREAM(MODIFIER|||||MIR1302-10|miRNA|NON_CODING|ENST00000408384|),INTRON(MODIFIER|||||MIR1302-10|antisense|NON_CODING|ENST00000469289|1),INTRON(MODIFIER|||||MIR1302-10|antisense|NON_CODING|ENST00000473358|2),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000423562|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000430492|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000438504|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000488147|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000538476|);CSQ=intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000243485|MIR1302-10|ENST00000473358|||||lincRNA|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000243485|MIR1302-10|ENST00000469289|||||lincRNA|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000488147|||||unprocessed_pseudogene|1,downstream_gene_variant|||ENSG00000237613|FAM138A|ENST00000417324|||||lincRNA|1,downstream_gene_variant|||ENSG00000243485|MIR1302-10|ENST00000607096|||||miRNA|1,downstream_gene_variant|||ENSG00000237613|FAM138A|ENST00000461467|||||lincRNA|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000538476|||||unprocessed_pseudogene|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000438504|||||unprocessed_pseudogene|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000423562|||||unprocessed_pseudogene|1 -chr1 30895 . T C 51.79 . AC=4;AF=0.069;AN=58;BaseQRankSum=-0.230;DP=63;Dels=0.00;FS=11.041;HRun=0;HaplotypeScore=0.2091;InbreedingCoeff=-0.0237;MQ=36.09;MQ0=0;MQRankSum=1.212;QD=3.98;ReadPosRankSum=0.230;EFF=DOWNSTREAM(MODIFIER||||85|FAM138A|protein_coding|CODING|ENST00000417324|),DOWNSTREAM(MODIFIER|||||FAM138A|processed_transcript|CODING|ENST00000461467|),DOWNSTREAM(MODIFIER|||||MIR1302-10|miRNA|NON_CODING|ENST00000408384|),INTRON(MODIFIER|||||MIR1302-10|antisense|NON_CODING|ENST00000469289|1),INTRON(MODIFIER|||||MIR1302-10|antisense|NON_CODING|ENST00000473358|2),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000423562|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000430492|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000438504|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000488147|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000538476|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000243485|MIR1302-10|ENST00000473358|||||lincRNA|1,intron_variant&nc_transcript_variant|||ENSG00000243485|MIR1302-10|ENST00000469289|||||lincRNA|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000488147|||||unprocessed_pseudogene|1,downstream_gene_variant|||ENSG00000237613|FAM138A|ENST00000417324|||||lincRNA|1,downstream_gene_variant|||ENSG00000243485|MIR1302-10|ENST00000607096|||||miRNA|1,downstream_gene_variant|||ENSG00000237613|FAM138A|ENST00000461467|||||lincRNA|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000538476|||||unprocessed_pseudogene|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000438504|||||unprocessed_pseudogene|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000423562|||||unprocessed_pseudogene|1 -chr1 30923 . G T 601.49 . AC=30;AF=1.000;AN=30;DP=17;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=-0.1858;MQ=35.61;MQ0=0;QD=35.38;EFF=DOWNSTREAM(MODIFIER||||85|FAM138A|protein_coding|CODING|ENST00000417324|),DOWNSTREAM(MODIFIER|||||FAM138A|processed_transcript|CODING|ENST00000461467|),DOWNSTREAM(MODIFIER|||||MIR1302-10|miRNA|NON_CODING|ENST00000408384|),INTRON(MODIFIER|||||MIR1302-10|antisense|NON_CODING|ENST00000469289|1),INTRON(MODIFIER|||||MIR1302-10|antisense|NON_CODING|ENST00000473358|2),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000423562|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000430492|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000438504|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000488147|),UPSTREAM(MODIFIER|||||WASH7P|unprocessed_pseudogene|NON_CODING|ENST00000538476|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000243485|MIR1302-10|ENST00000473358|||||lincRNA|1,intron_variant&nc_transcript_variant|||ENSG00000243485|MIR1302-10|ENST00000469289|||||lincRNA|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000488147|||||unprocessed_pseudogene|1,downstream_gene_variant|||ENSG00000237613|FAM138A|ENST00000417324|||||lincRNA|1,downstream_gene_variant|||ENSG00000243485|MIR1302-10|ENST00000607096|||||miRNA|1,downstream_gene_variant|||ENSG00000237613|FAM138A|ENST00000461467|||||lincRNA|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000538476|||||unprocessed_pseudogene|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000438504|||||unprocessed_pseudogene|1,upstream_gene_variant|||ENSG00000227232|WASH7P|ENST00000423562|||||unprocessed_pseudogene|1 -chr1 69270 . A G 2971.02 . AC=53;AF=0.946;AN=56;BaseQRankSum=1.675;DP=100;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0340;InbreedingCoeff=0.1553;MQ=31.06;MQ0=0;MQRankSum=-0.742;QD=31.61;ReadPosRankSum=-1.925;EFF=SYNONYMOUS_CODING(LOW|SILENT|tcA/tcG|S60|305|OR4F5|protein_coding|CODING|ENST00000335137|);CSQ=synonymous_variant|tcA/tcG|S|ENSG00000186092|OR4F5|ENST00000335137|1/1|||60/305|protein_coding|1 -chr1 69428 . T G 938.56 . AC=3;AF=0.0283;AN=106;BaseQRankSum=-0.638;DP=5013;DS;Dels=0.00;FS=2.607;HRun=0;HaplotypeScore=2.5603;InbreedingCoeff=0.3557;MQ=29.79;MQ0=0;MQRankSum=-5.524;QD=18.40;ReadPosRankSum=4.047;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tTt/tGt|F113C|305|OR4F5|protein_coding|CODING|ENST00000335137|);CSQ=missense_variant|tTt/tGt|F/C|ENSG00000186092|OR4F5|ENST00000335137|1/1|possibly_damaging(0.568)|deleterious(0)|113/305|protein_coding|1 -chr1 69511 . A G 101944.44 . AC=79;AF=0.878;AN=90;BaseQRankSum=-1.592;DP=3853;DS;Dels=0.00;FS=20.586;HRun=0;HaplotypeScore=3.0494;InbreedingCoeff=0.1357;MQ=33.24;MQ0=0;MQRankSum=-12.858;QD=26.51;ReadPosRankSum=-1.756;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Aca/Gca|T141A|305|OR4F5|protein_coding|CODING|ENST00000335137|);CSQ=missense_variant|Aca/Gca|T/A|ENSG00000186092|OR4F5|ENST00000335137|1/1|benign(0.003)|tolerated(0.63)|141/305|protein_coding|1 -chr1 69761 . A T 1026.71 . AC=8;AF=0.091;AN=88;BaseQRankSum=1.333;DP=574;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.3806;InbreedingCoeff=0.5887;MQ=32.18;MQ0=0;MQRankSum=-2.342;QD=21.39;ReadPosRankSum=-3.271;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAt/gTt|D224V|305|OR4F5|protein_coding|CODING|ENST00000335137|);CSQ=missense_variant|gAt/gTt|D/V|ENSG00000186092|OR4F5|ENST00000335137|1/1|benign(0.072)|tolerated(0.1)|224/305|protein_coding|1 -chr1 69871 . G A 82.97 . AC=2;AF=0.025;AN=80;BaseQRankSum=-0.646;DP=154;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.1095;InbreedingCoeff=0.1693;MQ=31.10;MQ0=0;MQRankSum=-0.154;QD=20.74;ReadPosRankSum=-0.292;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gat/Aat|D261N|305|OR4F5|protein_coding|CODING|ENST00000335137|);CSQ=missense_variant|Gat/Aat|D/N|ENSG00000186092|OR4F5|ENST00000335137|1/1|probably_damaging(0.968)|tolerated(0.06)|261/305|protein_coding|1 -chr1 69897 . T C 2544.15 . AC=52;AF=0.813;AN=64;BaseQRankSum=-0.912;DP=92;Dels=0.00;FS=58.362;HRun=1;HaplotypeScore=0.1549;InbreedingCoeff=0.0561;MQ=30.68;MQ0=0;MQRankSum=3.326;QD=28.59;ReadPosRankSum=1.059;EFF=SYNONYMOUS_CODING(LOW|SILENT|tcT/tcC|S269|305|OR4F5|protein_coding|CODING|ENST00000335137|);CSQ=synonymous_variant|tcT/tcC|S|ENSG00000186092|OR4F5|ENST00000335137|1/1|||269/305|protein_coding|1 -chr1 861008 . G C 1110.54 . AC=40;AF=0.870;AN=46;BaseQRankSum=1.889;DP=38;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=0.1855;MQ=56.89;MQ0=0;MQRankSum=1.660;QD=33.65;ReadPosRankSum=-1.946;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|1),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|1),UPSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1,upstream_gene_variant|||ENSG00000223764|RP11-54O7.3|ENST00000609207|||||retained_intron|1 -chr1 861605 . C T 52.33 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-0.110;DP=330;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.2194;InbreedingCoeff=-0.0688;MQ=57.00;MQ0=0;MQRankSum=-0.597;QD=10.47;ReadPosRankSum=-1.210;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|2),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|2),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|2);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 861630 . G A 5677.14 . AC=75;AF=0.7212;AN=104;BaseQRankSum=-3.827;DP=239;Dels=0.00;FS=4.448;HRun=0;HaplotypeScore=0.6050;InbreedingCoeff=0.2720;MQ=57.64;MQ0=0;MQRankSum=-2.334;QD=28.82;ReadPosRankSum=-1.755;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|2),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|2),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|2);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 861808 . A G 1231.98 . AC=34;AF=1.000;AN=34;DP=36;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0587;InbreedingCoeff=-0.1349;MQ=57.39;MQ0=0;QD=34.22;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|2),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|2),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|2);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 864938 . G A 38.62 . AC=4;AF=0.67;AN=6;BaseQRankSum=0.736;DP=3;Dels=0.00;FS=4.771;HRun=0;HaplotypeScore=0.0000;MQ=60.00;MQ0=0;MQRankSum=-0.736;QD=19.31;ReadPosRankSum=0.736;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|2),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|2),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|2);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 865219 . G A 271.52 . AC=14;AF=0.226;AN=62;BaseQRankSum=-0.335;DP=65;Dels=0.00;FS=1.303;HRun=0;HaplotypeScore=0.1417;InbreedingCoeff=0.1395;MQ=57.84;MQ0=0;MQRankSum=0.625;QD=14.29;ReadPosRankSum=-0.407;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|2),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|2),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|2);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 865367 . G T 48.93 . AC=2;AF=0.022;AN=90;BaseQRankSum=-0.637;DP=186;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3749;InbreedingCoeff=0.1476;MQ=55.90;MQ0=0;MQRankSum=2.051;QD=16.31;ReadPosRankSum=0.013;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|2),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|2),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|2);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 865435 . C G 566.25 . AC=2;AF=0.0185;AN=108;BaseQRankSum=1.312;DP=479;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6697;InbreedingCoeff=-0.0645;MQ=56.23;MQ0=0;MQRankSum=-0.467;QD=18.88;ReadPosRankSum=-0.530;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|2),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|2),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|2);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 865694 . C T 2783.07 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-1.642;DP=2811;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.6308;InbreedingCoeff=-0.0276;MQ=58.79;MQ0=0;MQRankSum=1.333;QD=13.71;ReadPosRankSum=0.518;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cac/Tac|H78Y|108|SAMD11|protein_coding|CODING|ENST00000437963|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cac/Tac|H78Y|178|SAMD11|protein_coding|CODING|ENST00000420190|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cac/Tac|H78Y|681|SAMD11|protein_coding|CODING|ENST00000342066|);CSQ=missense_variant|Cac/Tac|H/Y|ENSG00000187634|SAMD11|ENST00000420190|3/7|possibly_damaging(0.493)|tolerated(0.09)|78/179|protein_coding|1,missense_variant|tGc/tAc|C/Y|ENSG00000268179|AL645608.1|ENST00000598827|3/6|possibly_damaging(0.598)||18/112|protein_coding|1,missense_variant|Cac/Tac|H/Y|ENSG00000187634|SAMD11|ENST00000437963|3/5|possibly_damaging(0.493)|tolerated(0.12)|78/109|protein_coding|1,missense_variant|Cac/Tac|H/Y|ENSG00000187634|SAMD11|ENST00000342066|3/14|possibly_damaging(0.493)|tolerated(0.07)|78/681|protein_coding|1,missense_variant|Cac/Tac|H/Y|ENSG00000187634|SAMD11|ENST00000341065|1/12|probably_damaging(0.986)|deleterious(0)|2/589|protein_coding|1 -chr1 865738 . A G 389.63 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.098;DP=2190;Dels=0.00;FS=12.943;HRun=2;HaplotypeScore=1.1793;InbreedingCoeff=-0.0108;MQ=58.92;MQ0=0;MQRankSum=1.104;QD=9.28;ReadPosRankSum=0.259;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|3),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|3),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|3);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 866319 . G A 53469.99 . AC=118;AF=0.9833;AN=120;BaseQRankSum=1.591;DP=1731;Dels=0.00;FS=7.888;HRun=0;HaplotypeScore=1.0922;InbreedingCoeff=-0.0237;MQ=56.60;MQ0=0;MQRankSum=0.921;QD=30.89;ReadPosRankSum=-0.721;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|3),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|3),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|3);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 866511 . C CCCCT 58244.04 . AC=74;AF=0.6167;AN=120;BaseQRankSum=-16.318;DP=2662;FS=36.325;HRun=0;HaplotypeScore=234.1454;InbreedingCoeff=-0.0915;MQ=48.89;MQ0=0;MQRankSum=-26.717;QD=25.31;ReadPosRankSum=-3.550;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 866515 . T TCCCC 28452.17 . AC=52;AF=0.4333;AN=120;BaseQRankSum=20.767;DP=2538;FS=131.119;HRun=4;HaplotypeScore=272.7313;InbreedingCoeff=-0.6682;MQ=48.42;MQ0=0;MQRankSum=-29.484;QD=13.08;ReadPosRankSum=13.879;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 866517 . C G 804.92 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.085;DP=2503;Dels=0.00;FS=17.839;HRun=0;HaplotypeScore=16.1376;InbreedingCoeff=-0.0112;MQ=48.27;MQ0=0;MQRankSum=7.710;QD=18.29;ReadPosRankSum=2.729;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 866527 . A T 4017.89 . AC=45;AF=0.3750;AN=120;BaseQRankSum=11.973;DP=2186;Dels=0.00;FS=0.676;HRun=0;HaplotypeScore=3.9960;InbreedingCoeff=-0.5937;MQ=46.89;MQ0=0;MQRankSum=0.035;QD=2.40;ReadPosRankSum=-21.689;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 866557 . C T 127.71 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.174;DP=1561;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7669;InbreedingCoeff=-0.0117;MQ=49.50;MQ0=0;MQRankSum=3.050;QD=5.55;ReadPosRankSum=-0.277;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 866731 . G C 56.38 . AC=1;AF=0.011;AN=90;BaseQRankSum=-0.423;DP=139;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1598;InbreedingCoeff=-0.1061;MQ=53.72;MQ0=0;MQRankSum=0.762;QD=14.09;ReadPosRankSum=-0.131;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 866893 . T C 622.24 . AC=22;AF=0.500;AN=44;BaseQRankSum=-3.378;DP=42;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0452;InbreedingCoeff=0.1880;MQ=58.67;MQ0=0;MQRankSum=1.917;QD=23.93;ReadPosRankSum=-1.917;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 866920 . A G 1246.22 . AC=38;AF=1.000;AN=38;DP=41;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0415;InbreedingCoeff=-0.1594;MQ=58.06;MQ0=0;QD=32.80;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 866938 . G A 218.0 . AC=10;AF=0.278;AN=36;BaseQRankSum=1.425;DP=38;Dels=0.00;FS=4.047;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=0.1861;MQ=59.15;MQ0=0;MQRankSum=0.256;QD=18.17;ReadPosRankSum=-1.863;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 870645 . T C 290.54 . AC=16;AF=0.320;AN=50;BaseQRankSum=-0.425;DP=42;Dels=0.00;FS=3.173;HRun=0;HaplotypeScore=0.0800;InbreedingCoeff=0.0985;MQ=58.33;MQ0=0;MQRankSum=0.337;QD=13.21;ReadPosRankSum=1.918;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 870659 . G T 39.74 . AC=2;AF=0.040;AN=50;BaseQRankSum=1.431;DP=38;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0399;InbreedingCoeff=-0.0066;MQ=57.53;MQ0=0;MQRankSum=0.000;QD=13.25;ReadPosRankSum=1.202;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 870903 . T C 4527.15 . AC=105;AF=0.9722;AN=108;BaseQRankSum=-1.547;DP=151;Dels=0.01;FS=0.000;HRun=0;HaplotypeScore=0.3492;InbreedingCoeff=-0.0281;MQ=55.90;MQ0=0;MQRankSum=-1.281;QD=30.59;ReadPosRankSum=-1.215;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 871020 . G A 123.6 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.361;DP=922;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6728;InbreedingCoeff=-0.0124;MQ=58.46;MQ0=0;MQRankSum=0.878;QD=12.36;ReadPosRankSum=0.658;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 871042 . C CA 1121.53 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-3.932;DP=1196;FS=4.192;HRun=1;HaplotypeScore=68.0502;InbreedingCoeff=-0.0178;MQ=58.64;MQ0=0;MQRankSum=0.039;QD=20.77;ReadPosRankSum=1.478;EFF=INTRON(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|4),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|4),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|4),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 871215 . C G 3347.1 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.247;DP=4311;Dels=0.00;FS=7.397;HRun=1;HaplotypeScore=1.9160;InbreedingCoeff=-0.0257;MQ=58.16;MQ0=0;MQRankSum=2.137;QD=13.44;ReadPosRankSum=0.832;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccG|P123|178|SAMD11|protein_coding|CODING|ENST00000420190|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccG|P123|681|SAMD11|protein_coding|CODING|ENST00000342066|),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,synonymous_variant|ccC/ccG|P|ENSG00000187634|SAMD11|ENST00000420190|5/7|||123/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,synonymous_variant|ccC/ccG|P|ENSG00000187634|SAMD11|ENST00000342066|5/14|||123/681|protein_coding|1,synonymous_variant|ccC/ccG|P|ENSG00000187634|SAMD11|ENST00000341065|3/12|||47/589|protein_coding|1 -chr1 871306 . G A 333.26 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.027;DP=2068;Dels=0.00;FS=3.159;HRun=0;HaplotypeScore=2.0683;InbreedingCoeff=-0.0097;MQ=57.05;MQ0=0;MQRankSum=0.683;QD=9.52;ReadPosRankSum=0.829;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|5),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|5),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 871334 . G T 28303.7 . AC=78;AF=0.6500;AN=120;BaseQRankSum=-4.675;DP=1477;Dels=0.00;FS=1.219;HRun=0;HaplotypeScore=0.8602;InbreedingCoeff=-0.1122;MQ=57.60;MQ0=0;MQRankSum=3.104;QD=21.15;ReadPosRankSum=-0.389;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|5),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|5),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 871406 . C T 373.38 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.644;DP=663;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.3801;InbreedingCoeff=-0.0188;MQ=58.43;MQ0=0;MQRankSum=0.382;QD=14.94;ReadPosRankSum=0.041;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|5),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|5),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,upstream_gene_variant|||ENSG00000268179|AL645608.1|ENST00000598827||||-/112|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 871513 . C T 90.88 . AC=2;AF=0.0196;AN=102;BaseQRankSum=0.045;DP=143;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1691;InbreedingCoeff=0.0569;MQ=57.57;MQ0=0;MQRankSum=-0.474;QD=22.72;ReadPosRankSum=-0.123;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|5),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|5),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1 -chr1 871683 . G A 69.81 . AC=6;AF=0.375;AN=16;BaseQRankSum=0.684;DP=8;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.2498;MQ=58.46;MQ0=0;MQRankSum=1.221;QD=23.27;ReadPosRankSum=0.684;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|5),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|5),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1 -chr1 874314 . G A 306.48 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.210;DP=904;Dels=0.00;FS=2.007;HRun=2;HaplotypeScore=0.6052;InbreedingCoeff=-0.0150;MQ=58.05;MQ0=0;MQRankSum=-1.582;QD=10.95;ReadPosRankSum=0.649;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|5),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|5),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 874415 . C T 459.1 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.071;DP=2210;Dels=0.00;FS=0.832;HRun=0;HaplotypeScore=1.8123;InbreedingCoeff=-0.0101;MQ=58.23;MQ0=0;MQRankSum=1.495;QD=12.08;ReadPosRankSum=0.643;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),INTRON(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|5),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|5),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 874665 . G A 1190.84 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.904;DP=2083;Dels=0.00;FS=5.872;HRun=0;HaplotypeScore=1.2968;InbreedingCoeff=-0.0278;MQ=57.54;MQ0=0;MQRankSum=1.122;QD=14.35;ReadPosRankSum=0.856;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),SYNONYMOUS_CODING(LOW|SILENT|ctG/ctA|L177|178|SAMD11|protein_coding|CODING|ENST00000420190|),SYNONYMOUS_CODING(LOW|SILENT|ctG/ctA|L177|681|SAMD11|protein_coding|CODING|ENST00000342066|),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=synonymous_variant|ctG/ctA|L|ENSG00000187634|SAMD11|ENST00000420190|7/7|||177/179|protein_coding|1,synonymous_variant|ctG/ctA|L|ENSG00000187634|SAMD11|ENST00000342066|7/14|||177/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,synonymous_variant|ctG/ctA|L|ENSG00000187634|SAMD11|ENST00000455979|1/7|||4/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,synonymous_variant|ctG/ctA|L|ENSG00000187634|SAMD11|ENST00000341065|5/12|||101/589|protein_coding|1 -chr1 874816 . C CT 2431.73 . AC=13;AF=0.1083;AN=120;BaseQRankSum=-3.645;DP=2129;FS=0.000;HRun=1;HaplotypeScore=136.3097;InbreedingCoeff=0.2233;MQ=55.95;MQ0=0;MQRankSum=-0.937;QD=9.11;ReadPosRankSum=-4.095;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),FRAME_SHIFT(HIGH||-/T|-228?|681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,frameshift_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000342066|7/14|||228/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,frameshift_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000455979|1/7|||55/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 874817 . C T 196.11 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-3.463;DP=2129;Dels=0.00;FS=3.213;HRun=0;HaplotypeScore=3.1349;InbreedingCoeff=-0.0440;MQ=55.95;MQ0=0;MQRankSum=1.345;QD=2.84;ReadPosRankSum=-3.888;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCc/cTc|P228L|681|SAMD11|protein_coding|CODING|ENST00000342066|),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,missense_variant|cCc/cTc|P/L|ENSG00000187634|SAMD11|ENST00000342066|7/14|benign(0.003)|tolerated(0.26)|228/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,missense_variant|cCc/cTc|P/L|ENSG00000187634|SAMD11|ENST00000455979|1/7|benign(0.086)|tolerated(0.06)|55/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 874825 . C G 56.5 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.967;DP=2039;Dels=0.00;FS=3.631;HRun=0;HaplotypeScore=3.2093;InbreedingCoeff=-0.0100;MQ=55.77;MQ0=0;MQRankSum=0.586;QD=4.71;ReadPosRankSum=-3.947;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cac/Gac|H231D|681|SAMD11|protein_coding|CODING|ENST00000342066|),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,missense_variant|Cac/Gac|H/D|ENSG00000187634|SAMD11|ENST00000342066|7/14|benign(0)|tolerated(0.23)|231/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,missense_variant|Cac/Gac|H/D|ENSG00000187634|SAMD11|ENST00000455979|1/7|benign(0.001)|tolerated(0.22)|58/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 874826 . A G 43.49 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-4.114;DP=2017;Dels=0.00;FS=12.409;HRun=0;HaplotypeScore=2.9313;InbreedingCoeff=-0.0145;MQ=55.77;MQ0=0;MQRankSum=1.636;QD=3.62;ReadPosRankSum=-1.153;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cAc/cGc|H231R|681|SAMD11|protein_coding|CODING|ENST00000342066|),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,missense_variant|cAc/cGc|H/R|ENSG00000187634|SAMD11|ENST00000342066|7/14|benign(0)|tolerated(0.37)|231/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,missense_variant|cAc/cGc|H/R|ENSG00000187634|SAMD11|ENST00000455979|1/7|benign(0)|tolerated(0.36)|58/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 874864 . CT C 864.62 . AC=4;AF=0.0333;AN=120;BaseQRankSum=2.646;DP=1615;FS=13.206;HRun=1;HaplotypeScore=111.2623;InbreedingCoeff=-0.0402;MQ=54.48;MQ0=0;MQRankSum=-2.660;QD=5.84;ReadPosRankSum=-2.656;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 874878 . T C 30.73 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-11.417;DP=1417;Dels=0.00;FS=9.439;HRun=4;HaplotypeScore=1.6844;InbreedingCoeff=-0.0402;MQ=53.55;MQ0=0;MQRankSum=0.037;QD=0.29;ReadPosRankSum=-3.513;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 874927 . A G 459.72 . AC=5;AF=0.0439;AN=114;BaseQRankSum=2.529;DP=728;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5050;InbreedingCoeff=0.1366;MQ=49.13;MQ0=0;MQRankSum=-1.720;QD=7.93;ReadPosRankSum=0.425;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 874933 . G A 71.83 . AC=2;AF=0.0175;AN=114;BaseQRankSum=-2.598;DP=677;Dels=0.00;FS=12.021;HRun=0;HaplotypeScore=0.5171;InbreedingCoeff=0.3582;MQ=48.55;MQ0=0;MQRankSum=-2.054;QD=17.96;ReadPosRankSum=-0.568;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 874949 . G GAC 180.07 . AC=12;AF=0.1091;AN=110;BaseQRankSum=4.227;DP=513;FS=1.980;HRun=0;HaplotypeScore=80.8744;InbreedingCoeff=-0.1326;MQ=45.53;MQ0=0;MQRankSum=0.812;QD=1.55;ReadPosRankSum=-6.278;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 874950 . T C 171.91 . AC=11;AF=0.1000;AN=110;BaseQRankSum=-0.909;DP=513;Dels=0.00;FS=2.116;HRun=3;HaplotypeScore=3.7881;InbreedingCoeff=-0.1427;MQ=45.53;MQ0=0;MQRankSum=5.553;QD=1.55;ReadPosRankSum=-4.349;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 874950 . T TCCCTGGAGGACC 52769.69 . AC=82;AF=0.7455;AN=110;BaseQRankSum=-5.074;DP=556;FS=0.992;HRun=0;HaplotypeScore=89.5226;InbreedingCoeff=-0.2281;MQ=43.74;MQ0=0;MQRankSum=-13.823;QD=95.42;ReadPosRankSum=1.894;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 874955 . C G 162.26 . AC=15;AF=0.1364;AN=110;BaseQRankSum=-1.052;DP=411;Dels=0.00;FS=15.434;HRun=0;HaplotypeScore=3.6422;InbreedingCoeff=-0.1058;MQ=46.15;MQ0=0;MQRankSum=4.641;QD=1.62;ReadPosRankSum=-7.007;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 875159 . AGCCAGTGGACGCCGACCT A 727.04 . AC=15;AF=0.500;AN=30;BaseQRankSum=3.932;DP=27;FS=0.000;HRun=0;HaplotypeScore=24.4581;InbreedingCoeff=0.3261;MQ=51.25;MQ0=0;MQRankSum=-2.825;QD=80.78;ReadPosRankSum=2.534;EFF=DOWNSTREAM(MODIFIER||||108|SAMD11|protein_coding|CODING|ENST00000437963|),DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),UPSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000437963||||-/109|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 876498 . GA G 1427.8 . AC=48;AF=0.4000;AN=120;BaseQRankSum=-5.371;DP=794;FS=21.973;HRun=1;HaplotypeScore=83.2253;InbreedingCoeff=-0.3157;MQ=58.06;MQ0=0;MQRankSum=0.970;QD=2.48;ReadPosRankSum=-12.769;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|1),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),INTRON(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|1),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000474461|1/4||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 876499 . A G 22430.37 . AC=114;AF=0.9500;AN=120;BaseQRankSum=2.512;DP=794;Dels=0.01;FS=0.566;HRun=6;HaplotypeScore=0.6449;InbreedingCoeff=-0.0691;MQ=58.06;MQ0=0;MQRankSum=0.580;QD=28.25;ReadPosRankSum=0.704;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|1),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|7),INTRON(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|1),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|1/4||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 876595 . C T 278.95 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.357;DP=1272;Dels=0.00;FS=1.349;HRun=0;HaplotypeScore=0.7550;InbreedingCoeff=-0.0197;MQ=57.15;MQ0=0;MQRankSum=0.060;QD=13.28;ReadPosRankSum=-2.131;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),EXON(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|2),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ccc/Tcc|P260S|681|SAMD11|protein_coding|CODING|ENST00000342066|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,missense_variant|Ccc/Tcc|P/S|ENSG00000187634|SAMD11|ENST00000342066|8/14|probably_damaging(0.987)|deleterious(0)|260/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|1/4||||retained_intron|1,missense_variant|Ccc/Tcc|P/S|ENSG00000187634|SAMD11|ENST00000455979|2/7|probably_damaging(0.999)|deleterious(0)|87/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000478729|2/3||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,missense_variant|Ccc/Tcc|P/S|ENSG00000187634|SAMD11|ENST00000341065|6/12|probably_damaging(0.999)|tolerated(0.05)|168/589|protein_coding|1 -chr1 876908 . G A 134.68 . AC=1;AF=0.013;AN=80;BaseQRankSum=1.386;DP=184;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.2023;InbreedingCoeff=-0.0861;MQ=56.39;MQ0=0;MQRankSum=0.341;QD=14.96;ReadPosRankSum=1.908;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|8),INTRON(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|2),INTRON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|1),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 877147 . G A 43.79 . AC=6;AF=0.273;AN=22;BaseQRankSum=-0.925;DP=17;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0000;InbreedingCoeff=0.2867;MQ=52.81;MQ0=0;MQRankSum=0.185;QD=14.60;ReadPosRankSum=0.740;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|8),INTRON(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|2),INTRON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|1),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),UPSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,upstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 877715 . C G 5039.18 . AC=98;AF=0.9423;AN=104;BaseQRankSum=1.292;DP=191;Dels=0.00;FS=2.185;HRun=3;HaplotypeScore=0.2807;InbreedingCoeff=-0.0534;MQ=53.91;MQ0=0;MQRankSum=-0.346;QD=26.66;ReadPosRankSum=0.008;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|1),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|9),INTRON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|1),INTRON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|2);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000464948|1/2||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 877782 . C G 59.43 . AC=4;AF=0.0357;AN=112;BaseQRankSum=-0.568;DP=212;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0665;InbreedingCoeff=0.0350;MQ=54.31;MQ0=0;MQRankSum=1.437;QD=5.40;ReadPosRankSum=0.130;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|1),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|9),INTRON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|1),INTRON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|2);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000464948|1/2||||retained_intron|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 877831 . T C 5022.11 . AC=102;AF=1.0000;AN=102;BaseQRankSum=1.709;DP=173;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.1329;InbreedingCoeff=-0.0991;MQ=53.24;MQ0=0;MQRankSum=1.638;QD=29.20;ReadPosRankSum=-0.772;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|1),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|2),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|3),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tgg/Cgg|W343R|681|SAMD11|protein_coding|CODING|ENST00000342066|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,missense_variant|Tgg/Cgg|W/R|ENSG00000187634|SAMD11|ENST00000342066|10/14|benign(0)|tolerated(1)|343/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000464948|1/2||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000466827|2/2||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|3/4||||retained_intron|1,missense_variant|Tgg/Cgg|W/R|ENSG00000187634|SAMD11|ENST00000455979|4/7|benign(0)|tolerated(1)|170/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,missense_variant|Tgg/Cgg|W/R|ENSG00000187634|SAMD11|ENST00000341065|8/12|benign(0)|tolerated(1)|251/589|protein_coding|1 -chr1 878314 . G C 762.28 . AC=11;AF=0.0917;AN=120;BaseQRankSum=0.711;DP=450;Dels=0.00;FS=2.520;HRun=0;HaplotypeScore=0.3685;InbreedingCoeff=-0.1321;MQ=57.76;MQ0=0;MQRankSum=1.717;QD=9.30;ReadPosRankSum=1.114;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|4),SYNONYMOUS_CODING(LOW|SILENT|ggG/ggC|G480|681|SAMD11|protein_coding|CODING|ENST00000342066|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,synonymous_variant|ggG/ggC|G|ENSG00000187634|SAMD11|ENST00000342066|11/14|||480/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|4/4||||retained_intron|1,synonymous_variant|ggG/ggC|G|ENSG00000187634|SAMD11|ENST00000455979|5/7|||307/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,synonymous_variant|ggG/ggC|G|ENSG00000187634|SAMD11|ENST00000341065|9/12|||388/589|protein_coding|1 -chr1 878331 . C T 170.27 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.516;DP=507;Dels=0.00;FS=13.852;HRun=0;HaplotypeScore=0.4065;InbreedingCoeff=-0.0252;MQ=57.83;MQ0=0;MQRankSum=0.921;QD=21.28;ReadPosRankSum=-1.361;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),EXON(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|4),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P486L|681|SAMD11|protein_coding|CODING|ENST00000342066|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,missense_variant|cCg/cTg|P/L|ENSG00000187634|SAMD11|ENST00000342066|11/14|benign(0.007)|tolerated(0.12)|486/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187634|SAMD11|ENST00000474461|4/4||||retained_intron|1,missense_variant|cCg/cTg|P/L|ENSG00000187634|SAMD11|ENST00000455979|5/7|benign(0.029)|tolerated(0.09)|313/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,missense_variant|cCg/cTg|P/L|ENSG00000187634|SAMD11|ENST00000341065|9/12|benign(0.006)|tolerated(0.18)|394/589|protein_coding|1 -chr1 878516 . GA G 725.28 . AC=4;AF=0.0333;AN=120;BaseQRankSum=4.091;DP=1587;FS=6.039;HRun=5;HaplotypeScore=109.1933;InbreedingCoeff=-0.0395;MQ=57.40;MQ0=0;MQRankSum=0.773;QD=10.67;ReadPosRankSum=1.454;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|11);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 878761 . A AG 102.98 . AC=2;AF=0.0167;AN=120;BaseQRankSum=0.641;DP=955;FS=3.593;HRun=7;HaplotypeScore=63.6389;InbreedingCoeff=-0.0174;MQ=56.57;MQ0=0;MQRankSum=0.356;QD=4.90;ReadPosRankSum=-0.194;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|12);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,splice_region_variant&intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,splice_region_variant&intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,splice_region_variant&intron_variant&feature_elongation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 878764 . G C 139.53 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.470;DP=938;Dels=0.00;FS=7.307;HRun=0;HaplotypeScore=0.9091;InbreedingCoeff=-0.0119;MQ=56.61;MQ0=0;MQRankSum=1.683;QD=9.97;ReadPosRankSum=2.283;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|12);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 878904 . A C 629.76 . AC=27;AF=0.270;AN=100;BaseQRankSum=-10.394;DP=328;Dels=0.01;FS=66.455;HRun=4;HaplotypeScore=5.0793;InbreedingCoeff=-0.3279;MQ=45.67;MQ0=0;MQRankSum=-7.168;QD=2.59;ReadPosRankSum=7.847;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|12);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 878906 . CTTT C 498.81 . AC=35;AF=0.407;AN=86;BaseQRankSum=-0.896;DP=307;FS=10.838;HRun=22;HaplotypeScore=48.3314;InbreedingCoeff=0.1154;MQ=44.42;MQ0=0;MQRankSum=-1.401;QD=2.60;ReadPosRankSum=2.748;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|12);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 879195 . C T 744.73 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.733;DP=2228;Dels=0.00;FS=2.345;HRun=0;HaplotypeScore=2.3431;InbreedingCoeff=-0.0106;MQ=57.41;MQ0=0;MQRankSum=1.176;QD=11.82;ReadPosRankSum=0.278;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|13);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,missense_variant|Cgc/Tgc|R/C|ENSG00000187634|SAMD11|ENST00000455979|7/7|benign(0.047)|tolerated(0.15)|430/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 879317 . C T 1801.69 . AC=5;AF=0.0417;AN=120;BaseQRankSum=0.490;DP=1511;Dels=0.00;FS=0.480;HRun=0;HaplotypeScore=0.8559;InbreedingCoeff=-0.0439;MQ=57.96;MQ0=0;MQRankSum=-0.001;QD=13.15;ReadPosRankSum=0.731;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),SYNONYMOUS_CODING(LOW|SILENT|taC/taT|Y610|681|SAMD11|protein_coding|CODING|ENST00000342066|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,synonymous_variant|taC/taT|Y|ENSG00000187634|SAMD11|ENST00000342066|14/14|||610/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,synonymous_variant|taC/taT|Y|ENSG00000187634|SAMD11|ENST00000455979|7/7|||470/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,synonymous_variant|taC/taT|Y|ENSG00000187634|SAMD11|ENST00000341065|12/12|||518/589|protein_coding|1 -chr1 879376 . G A 35.51 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.159;DP=1323;Dels=0.00;FS=4.217;HRun=1;HaplotypeScore=0.6368;InbreedingCoeff=-0.0128;MQ=58.52;MQ0=0;MQRankSum=2.465;QD=2.96;ReadPosRankSum=0.209;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGa/cAa|R630Q|681|SAMD11|protein_coding|CODING|ENST00000342066|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,missense_variant|cGa/cAa|R/Q|ENSG00000187634|SAMD11|ENST00000342066|14/14|benign(0.048)|tolerated(0.13)|630/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,missense_variant|cGa/cAa|R/Q|ENSG00000187634|SAMD11|ENST00000455979|7/7|benign(0.024)|tolerated(0.19)|490/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,missense_variant|cGa/cAa|R/Q|ENSG00000187634|SAMD11|ENST00000341065|12/12|benign(0.012)|tolerated(0.16)|538/589|protein_coding|1 -chr1 879482 . G C 516.68 . AC=1;AF=0.0088;AN=114;BaseQRankSum=2.415;DP=1065;Dels=0.00;FS=3.604;HRun=0;HaplotypeScore=0.8043;InbreedingCoeff=-0.0358;MQ=59.14;MQ0=0;MQRankSum=0.854;QD=17.82;ReadPosRankSum=-1.823;EFF=DOWNSTREAM(MODIFIER||||178|SAMD11|protein_coding|CODING|ENST00000420190|),DOWNSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),SYNONYMOUS_CODING(LOW|SILENT|ggG/ggC|G665|681|SAMD11|protein_coding|CODING|ENST00000342066|);CSQ=downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000420190||||-/179|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,synonymous_variant|ggG/ggC|G|ENSG00000187634|SAMD11|ENST00000342066|14/14|||665/681|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,synonymous_variant|ggG/ggC|G|ENSG00000187634|SAMD11|ENST00000455979|7/7|||525/541|protein_coding|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,synonymous_variant|ggG/ggC|G|ENSG00000187634|SAMD11|ENST00000341065|12/12|||573/589|protein_coding|1 -chr1 879676 . G A 6945.69 . AC=71;AF=0.934;AN=76;BaseQRankSum=-1.592;DP=208;Dels=0.00;FS=0.000;HRun=3;HaplotypeScore=0.0908;InbreedingCoeff=0.0037;MQ=56.70;MQ0=0;MQRankSum=-0.599;QD=33.55;ReadPosRankSum=-1.965;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|17),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|5),UTR_3_PRIME(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|14),UTR_3_PRIME(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|19);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000187634|SAMD11|ENST00000342066|14/14|||-/681|protein_coding|1,3_prime_UTR_variant|||ENSG00000188976|NOC2L|ENST00000327044|19/19|||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|5/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|17/17||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187634|SAMD11|ENST00000341065|12/12|||-/589|protein_coding|1 -chr1 879687 . T C 5518.2 . AC=65;AF=0.956;AN=68;BaseQRankSum=0.725;DP=166;Dels=0.00;FS=1.578;HRun=1;HaplotypeScore=0.1048;InbreedingCoeff=0.0533;MQ=56.43;MQ0=0;MQRankSum=0.068;QD=33.44;ReadPosRankSum=-0.128;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|17),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|5),UTR_3_PRIME(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|14),UTR_3_PRIME(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|19);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000187634|SAMD11|ENST00000342066|14/14|||-/681|protein_coding|1,3_prime_UTR_variant|||ENSG00000188976|NOC2L|ENST00000327044|19/19|||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|5/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|17/17||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187634|SAMD11|ENST00000341065|12/12|||-/589|protein_coding|1 -chr1 879911 . G A 413.77 . AC=3;AF=0.0254;AN=118;BaseQRankSum=-0.760;DP=308;Dels=0.00;FS=1.528;HRun=1;HaplotypeScore=0.1788;InbreedingCoeff=-0.0710;MQ=57.83;MQ0=0;MQRankSum=0.760;QD=15.32;ReadPosRankSum=0.384;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|17),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|5),UTR_3_PRIME(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|14),UTR_3_PRIME(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|19);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000187634|SAMD11|ENST00000342066|14/14|||-/681|protein_coding|1,3_prime_UTR_variant|||ENSG00000188976|NOC2L|ENST00000327044|19/19|||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|5/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|17/17||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187634|SAMD11|ENST00000341065|12/12|||-/589|protein_coding|1 -chr1 880110 . G A 149.9 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.394;DP=1847;Dels=0.00;FS=1.624;HRun=0;HaplotypeScore=0.9203;InbreedingCoeff=-0.0094;MQ=57.47;MQ0=0;MQRankSum=1.220;QD=6.25;ReadPosRankSum=0.988;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|17),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|5),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D738|749|NOC2L|protein_coding|CODING|ENST00000327044|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,synonymous_variant|gaC/gaT|D|ENSG00000188976|NOC2L|ENST00000327044|19/19|||738/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|5/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|17/17||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 880238 . A G 43084.12 . AC=114;AF=0.9500;AN=120;BaseQRankSum=-0.613;DP=1203;Dels=0.00;FS=12.344;HRun=1;HaplotypeScore=1.0677;InbreedingCoeff=-0.0525;MQ=58.52;MQ0=0;MQRankSum=-1.859;QD=35.81;ReadPosRankSum=-0.535;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|18),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|4);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 880258 . TAA T 1233.06 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.890;DP=1382;FS=2.604;HRun=2;HaplotypeScore=131.0286;InbreedingCoeff=-0.0085;MQ=58.89;MQ0=0;MQRankSum=1.259;QD=20.55;ReadPosRankSum=-3.041;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|18),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|4);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 880367 . G A 795.59 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.447;DP=3095;Dels=0.00;FS=7.414;HRun=0;HaplotypeScore=2.5302;InbreedingCoeff=-0.0084;MQ=58.79;MQ0=0;MQRankSum=0.556;QD=10.61;ReadPosRankSum=0.729;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|18),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|4);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 880390 . C A 4207.41 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-1.360;DP=3838;Dels=0.00;FS=18.983;HRun=0;HaplotypeScore=2.5364;InbreedingCoeff=-0.0345;MQ=58.94;MQ0=0;MQRankSum=0.010;QD=14.76;ReadPosRankSum=1.464;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|18),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|4);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 880483 . A G 739.51 . AC=2;AF=0.0167;AN=120;BaseQRankSum=1.408;DP=5022;DS;Dels=0.00;FS=13.337;HRun=0;HaplotypeScore=2.6561;InbreedingCoeff=-0.0170;MQ=59.08;MQ0=0;MQRankSum=0.004;QD=12.75;ReadPosRankSum=-2.856;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|16),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|4),SYNONYMOUS_CODING(LOW|SILENT|gaT/gaC|D699|749|NOC2L|protein_coding|CODING|ENST00000327044|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,synonymous_variant|gaT/gaC|D|ENSG00000188976|NOC2L|ENST00000327044|18/19|||699/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|4/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|16/17||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 880639 . TC T 8144.14 . AC=31;AF=0.2583;AN=120;BaseQRankSum=8.550;DP=921;FS=0.348;HRun=2;HaplotypeScore=54.1306;InbreedingCoeff=-0.1017;MQ=58.66;MQ0=0;MQRankSum=1.840;QD=17.70;ReadPosRankSum=1.996;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|17),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|15),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|3);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 880751 . A G 807.9 . AC=4;AF=0.0333;AN=120;BaseQRankSum=1.558;DP=1112;Dels=0.00;FS=1.129;HRun=1;HaplotypeScore=0.7010;InbreedingCoeff=0.4309;MQ=58.97;MQ0=0;MQRankSum=3.000;QD=18.79;ReadPosRankSum=0.469;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|2),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|17),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|15),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|3);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000496938|2/2||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 881027 . C G 2780.96 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.239;DP=6197;DS;Dels=0.00;FS=8.993;HRun=0;HaplotypeScore=3.1641;InbreedingCoeff=-0.0084;MQ=59.26;MQ0=0;MQRankSum=2.376;QD=14.56;ReadPosRankSum=-0.209;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|15),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|3),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gac/Cac|D642H|749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,missense_variant|Gac/Cac|D/H|ENSG00000188976|NOC2L|ENST00000327044|17/19|probably_damaging(0.928)|deleterious(0)|642/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|3/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|15/17||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 881068 . G A 311.66 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.528;DP=3714;DS;Dels=0.00;FS=2.094;HRun=1;HaplotypeScore=2.7083;InbreedingCoeff=-0.0084;MQ=58.85;MQ0=0;MQRankSum=2.312;QD=14.17;ReadPosRankSum=-1.657;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|14),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|2),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 881214 . A G 121.77 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-0.359;DP=622;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.2865;InbreedingCoeff=-0.0200;MQ=58.09;MQ0=0;MQRankSum=-1.890;QD=15.22;ReadPosRankSum=-1.501;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|14),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|2),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 881278 . T C 73.18 . AC=5;AF=0.0472;AN=106;BaseQRankSum=-5.288;DP=225;Dels=0.00;FS=14.303;HRun=2;HaplotypeScore=0.2150;InbreedingCoeff=-0.1298;MQ=55.77;MQ0=0;MQRankSum=-0.007;QD=2.36;ReadPosRankSum=-0.143;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|14),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|2),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 881377 . G A 198.25 . AC=3;AF=0.0259;AN=116;BaseQRankSum=0.943;DP=299;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1931;InbreedingCoeff=-0.0772;MQ=57.25;MQ0=0;MQRankSum=1.239;QD=11.01;ReadPosRankSum=1.934;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|14),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|2),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 881512 . G T 329.45 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.087;DP=1882;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.4710;InbreedingCoeff=-0.0118;MQ=58.18;MQ0=0;MQRankSum=0.210;QD=11.36;ReadPosRankSum=-0.792;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|16),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|14),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|2),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 881627 . G A 65058.1 . AC=69;AF=0.5750;AN=120;BaseQRankSum=-0.436;DP=4175;Dels=0.00;FS=7.028;HRun=1;HaplotypeScore=3.6325;InbreedingCoeff=0.0788;MQ=58.73;MQ0=0;MQRankSum=0.133;QD=20.93;ReadPosRankSum=1.488;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|14),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|2),SYNONYMOUS_CODING(LOW|SILENT|Ctg/Ttg|L615|749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,synonymous_variant|Ctg/Ttg|L|ENSG00000188976|NOC2L|ENST00000327044|16/19|||615/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|2/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|14/17||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 881734 . T C 2395.2 . AC=3;AF=0.0250;AN=120;BaseQRankSum=1.087;DP=3324;Dels=0.00;FS=13.773;HRun=0;HaplotypeScore=2.3394;InbreedingCoeff=-0.0275;MQ=58.22;MQ0=0;MQRankSum=4.896;QD=14.34;ReadPosRankSum=1.344;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|15),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|13),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|1),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 881894 . G A 502.77 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.087;DP=3111;Dels=0.00;FS=0.806;HRun=0;HaplotypeScore=1.9434;InbreedingCoeff=-0.0084;MQ=55.95;MQ0=0;MQRankSum=4.130;QD=7.62;ReadPosRankSum=-0.465;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|13),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCc/gTc|A564V|749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,missense_variant|gCc/gTc|A/V|ENSG00000188976|NOC2L|ENST00000327044|15/19|probably_damaging(0.911)|deleterious(0)|564/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|1/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|13/17||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 881918 . G A 4148.23 . AC=6;AF=0.0500;AN=120;BaseQRankSum=0.336;DP=2717;Dels=0.00;FS=3.311;HRun=1;HaplotypeScore=1.8603;InbreedingCoeff=-0.0526;MQ=57.50;MQ0=0;MQRankSum=10.868;QD=13.38;ReadPosRankSum=2.821;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|13),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|tCg/tTg|S556L|749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,missense_variant|tCg/tTg|S/L|ENSG00000188976|NOC2L|ENST00000327044|15/19|benign(0.311)|deleterious(0.03)|556/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|1/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|13/17||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 882033 . G A 7803.05 . AC=29;AF=0.2417;AN=120;BaseQRankSum=-1.083;DP=803;Dels=0.00;FS=0.805;HRun=1;HaplotypeScore=0.5525;InbreedingCoeff=-0.0708;MQ=56.82;MQ0=0;MQRankSum=1.538;QD=18.99;ReadPosRankSum=-1.493;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),DOWNSTREAM(MODIFIER|||||SAMD11|processed_transcript|CODING|ENST00000478729|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000464948|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000466827|),DOWNSTREAM(MODIFIER|||||SAMD11|retained_intron|CODING|ENST00000474461|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|1),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|14),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|12),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000464948|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000466827|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000474461|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000483767|1/5||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000478729|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 883625 . A G 54754.53 . AC=120;AF=1.0000;AN=120;DP=1639;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=1.0098;InbreedingCoeff=-0.0052;MQ=58.06;MQ0=0;QD=33.41;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|13),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|11),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 883699 . C T 453.1 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.101;DP=923;Dels=0.00;FS=14.883;HRun=0;HaplotypeScore=0.5131;InbreedingCoeff=-0.0294;MQ=56.96;MQ0=0;MQRankSum=3.137;QD=12.25;ReadPosRankSum=2.276;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|13),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|11),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 883750 . T C 877.5 . AC=3;AF=0.0250;AN=120;BaseQRankSum=1.693;DP=1077;Dels=0.00;FS=5.318;HRun=3;HaplotypeScore=0.3559;InbreedingCoeff=-0.0265;MQ=57.03;MQ0=0;MQRankSum=4.377;QD=17.21;ReadPosRankSum=1.955;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|13),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|11),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 883960 . G A 483.87 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.195;DP=3321;Dels=0.00;FS=1.899;HRun=0;HaplotypeScore=1.8090;InbreedingCoeff=-0.0084;MQ=57.94;MQ0=0;MQRankSum=2.447;QD=10.30;ReadPosRankSum=1.425;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|11),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N489|749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,synonymous_variant|aaC/aaT|N|ENSG00000188976|NOC2L|ENST00000327044|13/19|||489/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|11/17||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 884013 . G A 359.69 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.049;DP=1892;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.9736;InbreedingCoeff=-0.0084;MQ=57.09;MQ0=0;MQRankSum=2.918;QD=14.99;ReadPosRankSum=0.004;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 884035 . G GCC 125.11 . AC=12;AF=0.1000;AN=120;BaseQRankSum=10.429;DP=1507;FS=5.790;HRun=1;HaplotypeScore=118.1286;InbreedingCoeff=-0.1150;MQ=55.10;MQ0=0;MQRankSum=-9.464;QD=0.53;ReadPosRankSum=-1.582;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant&feature_elongation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 884040 . AC A 54.58 . AC=10;AF=0.0833;AN=120;BaseQRankSum=10.370;DP=1485;FS=5.837;HRun=3;HaplotypeScore=105.2143;InbreedingCoeff=-0.0972;MQ=54.77;MQ0=0;MQRankSum=-6.138;QD=0.29;ReadPosRankSum=-3.294;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 884091 . C G 9412.98 . AC=59;AF=0.4917;AN=120;BaseQRankSum=0.144;DP=830;Dels=0.00;FS=3.690;HRun=1;HaplotypeScore=1.5824;InbreedingCoeff=-0.8297;MQ=49.85;MQ0=0;MQRankSum=3.025;QD=11.58;ReadPosRankSum=6.853;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 884101 . A C 9419.59 . AC=54;AF=0.4500;AN=120;BaseQRankSum=12.324;DP=697;Dels=0.00;FS=0.000;HRun=3;HaplotypeScore=1.3334;InbreedingCoeff=-0.7661;MQ=48.44;MQ0=0;MQRankSum=-6.895;QD=14.45;ReadPosRankSum=-14.010;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 884111 . T C 106.0 . AC=12;AF=0.1017;AN=118;BaseQRankSum=-1.701;DP=405;Dels=0.00;FS=32.333;HRun=2;HaplotypeScore=0.1962;InbreedingCoeff=-0.1423;MQ=53.01;MQ0=0;MQRankSum=1.879;QD=1.39;ReadPosRankSum=-6.750;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 884426 . A AACAGCAAAG 1975.5 . AC=28;AF=1.000;AN=28;DP=24;FS=0.000;HRun=0;HaplotypeScore=8.5806;InbreedingCoeff=-0.1820;MQ=37.89;MQ0=0;QD=98.77;EFF=DOWNSTREAM(MODIFIER||||681|SAMD11|protein_coding|CODING|ENST00000342066|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000496938|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000455979||||-/541|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000496938|||||processed_transcript|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000342066||||-/681|protein_coding|1,intron_variant&feature_elongation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000187634|SAMD11|ENST00000341065||||-/589|protein_coding|1 -chr1 886006 . T C 1030.61 . AC=32;AF=1.000;AN=32;DP=31;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=-0.1369;MQ=49.82;MQ0=0;QD=33.25;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 886049 . ACAG A 1495.02 . AC=34;AF=0.944;AN=36;BaseQRankSum=1.722;DP=33;FS=2.691;HRun=0;HaplotypeScore=10.4318;InbreedingCoeff=0.1010;MQ=41.07;MQ0=0;MQRankSum=-2.098;QD=49.83;ReadPosRankSum=0.344;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 886179 . CA C 1612.0 . AC=47;AF=0.870;AN=54;BaseQRankSum=3.364;DP=49;FS=8.293;HRun=1;HaplotypeScore=18.7687;InbreedingCoeff=0.1732;MQ=36.71;MQ0=0;MQRankSum=-3.123;QD=36.64;ReadPosRankSum=0.259;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 886182 . TG T 1532.98 . AC=47;AF=0.870;AN=54;BaseQRankSum=3.246;DP=46;FS=5.278;HRun=1;HaplotypeScore=18.4565;InbreedingCoeff=0.1421;MQ=34.65;MQ0=0;MQRankSum=-2.960;QD=36.50;ReadPosRankSum=0.184;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 886186 . C CAG 2268.91 . AC=57;AF=0.891;AN=64;BaseQRankSum=3.232;DP=55;FS=6.052;HRun=0;HaplotypeScore=17.8027;InbreedingCoeff=0.1016;MQ=34.53;MQ0=0;MQRankSum=-3.122;QD=44.49;ReadPosRankSum=0.055;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 886384 . G A 434.04 . AC=4;AF=0.0333;AN=120;BaseQRankSum=0.231;DP=475;Dels=0.00;FS=8.266;HRun=0;HaplotypeScore=0.2516;InbreedingCoeff=-0.0534;MQ=58.22;MQ0=0;MQRankSum=1.803;QD=11.42;ReadPosRankSum=-0.069;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 886409 . G C 225.93 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-0.047;DP=629;Dels=0.00;FS=1.803;HRun=2;HaplotypeScore=0.3393;InbreedingCoeff=-0.0500;MQ=58.47;MQ0=0;MQRankSum=0.500;QD=5.79;ReadPosRankSum=-0.845;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 886477 . G A 1109.56 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.561;DP=1412;Dels=0.00;FS=5.360;HRun=0;HaplotypeScore=1.3637;InbreedingCoeff=-0.0325;MQ=58.75;MQ0=0;MQRankSum=0.602;QD=11.56;ReadPosRankSum=-1.213;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 886492 . C T 237.45 . AC=2;AF=0.0167;AN=120;BaseQRankSum=0.074;DP=1595;Dels=0.00;FS=5.466;HRun=0;HaplotypeScore=0.8808;InbreedingCoeff=0.7925;MQ=58.78;MQ0=0;MQRankSum=0.397;QD=21.59;ReadPosRankSum=-0.482;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|12),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|10),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 886653 . G A 558.63 . AC=2;AF=0.0169;AN=118;BaseQRankSum=1.368;DP=1520;Dels=0.00;FS=21.500;HRun=0;HaplotypeScore=1.1951;InbreedingCoeff=-0.0285;MQ=58.36;MQ0=0;MQRankSum=0.809;QD=15.52;ReadPosRankSum=-1.905;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|11),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|9),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 886788 . G A 9988.89 . AC=98;AF=0.8305;AN=118;BaseQRankSum=-1.367;DP=334;Dels=0.00;FS=18.012;HRun=1;HaplotypeScore=0.1988;InbreedingCoeff=0.2452;MQ=57.31;MQ0=0;MQRankSum=4.299;QD=32.86;ReadPosRankSum=-0.071;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|11),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|9),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 886817 . C T 6332.01 . AC=90;AF=0.8182;AN=110;BaseQRankSum=-1.237;DP=216;Dels=0.00;FS=12.660;HRun=3;HaplotypeScore=0.3611;InbreedingCoeff=0.1623;MQ=57.12;MQ0=0;MQRankSum=2.897;QD=31.50;ReadPosRankSum=0.605;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|11),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|9),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 886817 . C CATTTT 1195.05 . AC=14;AF=0.1296;AN=108;BaseQRankSum=-6.408;DP=215;FS=1.303;HRun=0;HaplotypeScore=40.0482;InbreedingCoeff=0.0654;MQ=57.03;MQ0=0;MQRankSum=-3.623;QD=23.43;ReadPosRankSum=-3.297;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|11),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|9),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 887162 . T C 158.95 . AC=3;AF=0.0273;AN=110;BaseQRankSum=0.867;DP=242;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1059;InbreedingCoeff=-0.1008;MQ=57.95;MQ0=0;MQRankSum=1.543;QD=10.60;ReadPosRankSum=-1.064;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|11),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|9),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 887265 . C A 101.07 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.874;DP=895;Dels=0.00;FS=2.947;HRun=0;HaplotypeScore=0.4283;InbreedingCoeff=-0.0106;MQ=59.14;MQ0=0;MQRankSum=2.148;QD=6.74;ReadPosRankSum=-0.250;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|11),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|9),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000483767|);CSQ=upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000483767|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 887471 . G A 581.35 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.013;DP=3085;Dels=0.00;FS=3.856;HRun=1;HaplotypeScore=1.5844;InbreedingCoeff=-0.0084;MQ=59.14;MQ0=0;MQRankSum=3.944;QD=12.92;ReadPosRankSum=0.093;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|9),SYNONYMOUS_CODING(LOW|SILENT|Ctg/Ttg|L414|749|NOC2L|protein_coding|CODING|ENST00000327044|);CSQ=synonymous_variant|Ctg/Ttg|L|ENSG00000188976|NOC2L|ENST00000327044|11/19|||414/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|9/17||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 887537 . C T 1658.47 . AC=3;AF=0.0250;AN=120;BaseQRankSum=1.815;DP=2530;Dels=0.00;FS=6.149;HRun=0;HaplotypeScore=1.6593;InbreedingCoeff=-0.0258;MQ=59.28;MQ0=0;MQRankSum=2.238;QD=12.11;ReadPosRankSum=2.057;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|10),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|8);CSQ=intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 887560 . A C 70115.69 . AC=113;AF=0.9417;AN=120;BaseQRankSum=0.848;DP=2150;Dels=0.00;FS=10.744;HRun=1;HaplotypeScore=1.3105;InbreedingCoeff=0.2414;MQ=59.13;MQ0=0;MQRankSum=-0.778;QD=32.87;ReadPosRankSum=1.087;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|10),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|8);CSQ=intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 887651 . C T 665.1 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.019;DP=1257;Dels=0.00;FS=1.751;HRun=0;HaplotypeScore=0.9128;InbreedingCoeff=-0.0177;MQ=58.63;MQ0=0;MQRankSum=3.510;QD=17.50;ReadPosRankSum=2.127;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|10),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|8);CSQ=intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 887801 . A G 116758.48 . AC=115;AF=0.9583;AN=120;BaseQRankSum=4.615;DP=3778;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=2.1571;InbreedingCoeff=-0.0435;MQ=57.90;MQ0=0;MQRankSum=2.145;QD=30.90;ReadPosRankSum=1.213;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|8),SYNONYMOUS_CODING(LOW|SILENT|acT/acC|T394|749|NOC2L|protein_coding|CODING|ENST00000327044|);CSQ=synonymous_variant|acT/acC|T|ENSG00000188976|NOC2L|ENST00000327044|10/19|||394/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|8/17||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 888220 . G A 81.05 . AC=4;AF=0.044;AN=90;BaseQRankSum=-1.159;DP=137;Dels=0.00;FS=2.348;HRun=0;HaplotypeScore=0.1677;InbreedingCoeff=-0.0166;MQ=57.57;MQ0=0;MQRankSum=1.051;QD=7.37;ReadPosRankSum=1.796;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|9),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|7);CSQ=intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 888239 . C T 79.33 . AC=3;AF=0.034;AN=88;BaseQRankSum=0.324;DP=113;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0969;InbreedingCoeff=-0.0212;MQ=57.69;MQ0=0;MQRankSum=1.258;QD=13.22;ReadPosRankSum=-0.910;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|9),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|7);CSQ=intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 888639 . T C 94387.16 . AC=115;AF=0.9583;AN=120;BaseQRankSum=0.263;DP=3128;Dels=0.00;FS=2.314;HRun=1;HaplotypeScore=1.2974;InbreedingCoeff=-0.0435;MQ=54.95;MQ0=0;MQRankSum=0.465;QD=30.17;ReadPosRankSum=1.529;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|7),SYNONYMOUS_CODING(LOW|SILENT|gaA/gaG|E306|749|NOC2L|protein_coding|CODING|ENST00000327044|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,synonymous_variant|gaA/gaG|E|ENSG00000188976|NOC2L|ENST00000327044|9/19|||306/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|7/17||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 888659 . T C 80634.32 . AC=115;AF=0.9583;AN=120;BaseQRankSum=1.739;DP=2737;Dels=0.00;FS=2.276;HRun=1;HaplotypeScore=1.3379;InbreedingCoeff=-0.0436;MQ=54.78;MQ0=0;MQRankSum=-1.113;QD=29.46;ReadPosRankSum=4.132;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|7),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Atc/Gtc|I300V|749|NOC2L|protein_coding|CODING|ENST00000327044|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,missense_variant|Atc/Gtc|I/V|ENSG00000188976|NOC2L|ENST00000327044|9/19|benign(0)|tolerated(0.98)|300/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|7/17||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 889131 . A G 423.54 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-1.236;DP=1138;Dels=0.00;FS=7.564;HRun=1;HaplotypeScore=0.6841;InbreedingCoeff=-0.0300;MQ=57.89;MQ0=0;MQRankSum=0.858;QD=9.41;ReadPosRankSum=0.777;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|8),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|6);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 889158 . G C 40721.48 . AC=113;AF=0.9417;AN=120;BaseQRankSum=1.359;DP=1345;Dels=0.00;FS=0.512;HRun=1;HaplotypeScore=0.9432;InbreedingCoeff=0.2187;MQ=58.28;MQ0=0;MQRankSum=1.507;QD=30.55;ReadPosRankSum=0.613;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|8),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|6);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 889159 . A C 41244.53 . AC=113;AF=0.9417;AN=120;BaseQRankSum=1.309;DP=1361;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.8382;InbreedingCoeff=0.2198;MQ=58.26;MQ0=0;MQRankSum=1.567;QD=30.57;ReadPosRankSum=1.331;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|8),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|6);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 889238 . G A 1672.71 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-2.383;DP=1656;Dels=0.00;FS=10.199;HRun=0;HaplotypeScore=0.9421;InbreedingCoeff=-0.0479;MQ=58.37;MQ0=0;MQRankSum=0.456;QD=10.93;ReadPosRankSum=-1.085;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|6),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCg/gTg|A271V|749|NOC2L|protein_coding|CODING|ENST00000327044|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,missense_variant|gCg/gTg|A/V|ENSG00000188976|NOC2L|ENST00000327044|8/19|benign(0.034)|deleterious(0.04)|271/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|6/17||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 889638 . G C 14363.41 . AC=105;AF=0.9211;AN=114;BaseQRankSum=-0.922;DP=427;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3035;InbreedingCoeff=0.0466;MQ=57.19;MQ0=0;MQRankSum=0.153;QD=34.12;ReadPosRankSum=1.663;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|5),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|6);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|5/17||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 889713 . C A 5043.64 . AC=90;AF=0.918;AN=98;BaseQRankSum=-0.018;DP=155;Dels=0.00;FS=4.406;HRun=0;HaplotypeScore=0.0300;InbreedingCoeff=0.0572;MQ=56.80;MQ0=0;MQRankSum=1.329;QD=33.40;ReadPosRankSum=0.873;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|5),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|6);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|5/17||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 891021 . G A 7780.97 . AC=101;AF=0.9182;AN=110;BaseQRankSum=0.260;DP=242;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1735;InbreedingCoeff=-0.0202;MQ=58.75;MQ0=0;MQRankSum=-0.704;QD=33.11;ReadPosRankSum=0.580;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|6),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|6),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|4),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 891059 . C T 8049.27 . AC=70;AF=0.5932;AN=118;BaseQRankSum=0.106;DP=392;Dels=0.00;FS=5.538;HRun=2;HaplotypeScore=0.1844;InbreedingCoeff=0.1314;MQ=58.93;MQ0=0;MQRankSum=-2.097;QD=26.74;ReadPosRankSum=0.989;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|6),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|6),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|4),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 891401 . G A 2441.56 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.910;DP=6853;DS;Dels=0.00;FS=20.474;HRun=4;HaplotypeScore=2.8477;InbreedingCoeff=-0.0084;MQ=58.69;MQ0=0;MQRankSum=2.695;QD=14.45;ReadPosRankSum=2.367;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|5),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|5),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|3),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 891620 . C A 630.55 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.211;DP=3333;Dels=0.00;FS=17.146;HRun=0;HaplotypeScore=2.5646;InbreedingCoeff=-0.0084;MQ=59.15;MQ0=0;MQRankSum=0.979;QD=8.19;ReadPosRankSum=2.951;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|4),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|4),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|);CSQ=downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 891898 . T C 58.0 . AC=2;AF=0.026;AN=78;BaseQRankSum=-1.208;DP=130;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0256;InbreedingCoeff=-0.1375;MQ=58.94;MQ0=0;MQRankSum=0.462;QD=4.46;ReadPosRankSum=-1.373;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|4),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|4),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 891945 . A G 1861.84 . AC=44;AF=0.550;AN=80;BaseQRankSum=-1.331;DP=117;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.1025;InbreedingCoeff=0.1989;MQ=59.45;MQ0=0;MQRankSum=-0.841;QD=24.82;ReadPosRankSum=0.357;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|4),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|4),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 892095 . C T 592.96 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.068;DP=1006;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5011;InbreedingCoeff=-0.0379;MQ=59.11;MQ0=0;MQRankSum=0.441;QD=14.82;ReadPosRankSum=1.641;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|4),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|4),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 892141 . C T 66.06 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-2.417;DP=1605;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.9164;InbreedingCoeff=-0.0175;MQ=59.28;MQ0=0;MQRankSum=0.188;QD=3.15;ReadPosRankSum=0.854;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|4),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|4),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 892237 . C A 5045.9 . AC=2;AF=0.0167;AN=120;BaseQRankSum=2.206;DP=4638;DS;Dels=0.00;FS=16.397;HRun=0;HaplotypeScore=2.4024;InbreedingCoeff=-0.0170;MQ=59.24;MQ0=0;MQRankSum=2.024;QD=16.22;ReadPosRankSum=-3.730;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|4),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|4),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 892460 . G C 4504.79 . AC=5;AF=0.0417;AN=120;BaseQRankSum=2.877;DP=3730;Dels=0.00;FS=1.037;HRun=0;HaplotypeScore=2.4668;InbreedingCoeff=-0.0435;MQ=59.20;MQ0=0;MQRankSum=1.411;QD=15.17;ReadPosRankSum=0.828;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|3),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|3),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|1),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 892470 . C T 571.4 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.526;DP=3581;Dels=0.00;FS=5.690;HRun=0;HaplotypeScore=4.5258;InbreedingCoeff=-0.0084;MQ=59.15;MQ0=0;MQRankSum=0.418;QD=11.90;ReadPosRankSum=0.317;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|3),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|3),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|1),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 892471 . G A 2720.48 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-7.069;DP=3602;Dels=0.00;FS=2.243;HRun=0;HaplotypeScore=2.7847;InbreedingCoeff=0.4828;MQ=59.14;MQ0=0;MQRankSum=-0.017;QD=13.95;ReadPosRankSum=-0.355;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|3),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|3),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|1),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 892724 . C T 85.66 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.766;DP=1274;Dels=0.00;FS=3.424;HRun=1;HaplotypeScore=0.8538;InbreedingCoeff=-0.0064;MQ=58.33;MQ0=0;MQRankSum=0.065;QD=14.28;ReadPosRankSum=-0.872;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|1),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|2),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|1/17||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 892745 . G A 33455.23 . AC=115;AF=0.9583;AN=120;BaseQRankSum=-1.159;DP=989;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3493;InbreedingCoeff=-0.0478;MQ=58.30;MQ0=0;MQRankSum=0.300;QD=33.83;ReadPosRankSum=-0.595;EFF=DOWNSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|1),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|2),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,downstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|1/17||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 893719 . G A 126.7 . AC=10;AF=0.833;AN=12;BaseQRankSum=-0.198;DP=10;Dels=0.00;FS=3.680;HRun=0;HaplotypeScore=0.1223;MQ=57.66;MQ0=0;MQRankSum=-1.495;QD=21.12;ReadPosRankSum=0.198;EFF=EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|2),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|1),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|2),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000469563|2/2||||retained_intron|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000477976|1/17||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 894027 . G A 256.69 . AC=1;AF=0.012;AN=82;BaseQRankSum=-0.446;DP=427;Dels=0.00;FS=4.334;HRun=2;HaplotypeScore=0.2194;InbreedingCoeff=-0.0706;MQ=57.55;MQ0=0;MQRankSum=0.775;QD=18.34;ReadPosRankSum=-0.924;EFF=EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|2),INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|2),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|2),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000469563|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 894330 . G A 79.5 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.248;DP=1450;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7814;InbreedingCoeff=-0.0087;MQ=58.82;MQ0=0;MQRankSum=0.499;QD=7.23;ReadPosRankSum=0.368;EFF=EXON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|2),EXON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|2),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P53L|749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,missense_variant|cCg/cTg|P/L|ENSG00000188976|NOC2L|ENST00000327044|2/19|benign(0.001)|tolerated(0.41)|53/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000469563|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|2/7||||processed_transcript|1 -chr1 894519 . G A 127.69 . AC=2;AF=0.0167;AN=120;BaseQRankSum=0.782;DP=1334;Dels=0.00;FS=1.737;HRun=1;HaplotypeScore=0.3864;InbreedingCoeff=-0.0205;MQ=58.98;MQ0=0;MQRankSum=0.710;QD=6.72;ReadPosRankSum=0.813;EFF=INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|1),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|1),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|1),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 894573 . G A 31559.56 . AC=101;AF=0.8417;AN=120;BaseQRankSum=-0.317;DP=1219;Dels=0.00;FS=2.978;HRun=0;HaplotypeScore=0.8111;InbreedingCoeff=0.1756;MQ=58.45;MQ0=0;MQRankSum=-0.591;QD=27.47;ReadPosRankSum=-0.806;EFF=INTRON(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|1),INTRON(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|1),INTRON(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|1),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,intron_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 894719 . C T 134.46 . AC=5;AF=0.0463;AN=108;BaseQRankSum=-1.930;DP=230;Dels=0.00;FS=6.502;HRun=0;HaplotypeScore=0.2397;InbreedingCoeff=0.1830;MQ=54.07;MQ0=0;MQRankSum=-0.173;QD=8.40;ReadPosRankSum=1.316;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 894890 . A AAGAC 433.26 . AC=10;AF=1.00;AN=10;DP=6;FS=0.000;HRun=0;HaplotypeScore=9.9355;MQ=29.00;MQ0=0;QD=72.21;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 895706 . G A 654.83 . AC=24;AF=0.857;AN=28;BaseQRankSum=-0.382;DP=28;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.1332;InbreedingCoeff=0.2034;MQ=55.74;MQ0=0;MQRankSum=1.582;QD=26.19;ReadPosRankSum=1.255;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 895755 . A AG 836.52 . AC=32;AF=0.941;AN=34;BaseQRankSum=-1.595;DP=39;FS=0.000;HRun=6;HaplotypeScore=8.1200;InbreedingCoeff=0.0511;MQ=54.87;MQ0=0;MQRankSum=1.595;QD=24.60;ReadPosRankSum=-1.456;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 896476 . A G 230.97 . AC=14;AF=1.000;AN=14;DP=11;Dels=0.00;FS=0.000;HRun=4;HaplotypeScore=0.0000;MQ=57.87;MQ0=0;QD=21.00;EFF=INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|1),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|1),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 896950 . C A 1565.88 . AC=3;AF=0.0273;AN=110;BaseQRankSum=-1.726;DP=1397;Dels=0.00;FS=5.899;HRun=0;HaplotypeScore=0.8800;InbreedingCoeff=-0.0557;MQ=59.13;MQ0=0;MQRankSum=0.483;QD=13.27;ReadPosRankSum=2.665;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|1),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|1),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|2),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|1/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 896977 . G C 992.92 . AC=1;AF=0.0086;AN=116;BaseQRankSum=3.226;DP=1654;Dels=0.00;FS=64.681;HRun=1;HaplotypeScore=1.1124;InbreedingCoeff=-0.0302;MQ=59.13;MQ0=0;MQRankSum=0.693;QD=19.09;ReadPosRankSum=-3.945;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|1),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|1),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|2),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|1/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 897325 . G C 125121.37 . AC=109;AF=0.9083;AN=120;BaseQRankSum=2.562;DP=4268;Dels=0.00;FS=6.072;HRun=1;HaplotypeScore=2.5759;InbreedingCoeff=0.0992;MQ=58.65;MQ0=0;MQRankSum=-0.923;QD=29.72;ReadPosRankSum=-2.769;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),SYNONYMOUS_CODING(LOW|SILENT|gcG/gcC|A203|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|),UTR_5_PRIME(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|3);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,synonymous_variant|gcG/gcC|A|ENSG00000187961|KLHL17|ENST00000338591|4/12|||203/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 897337 . C T 1277.99 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.453;DP=4287;Dels=0.00;FS=8.422;HRun=0;HaplotypeScore=2.3521;InbreedingCoeff=-0.0084;MQ=58.59;MQ0=0;MQRankSum=0.646;QD=14.36;ReadPosRankSum=0.556;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),START_GAINED(LOW||||412|KLHL17|protein_coding|CODING|ENST00000455747|3),SYNONYMOUS_CODING(LOW|SILENT|agC/agT|S207|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,synonymous_variant|agC/agT|S|ENSG00000187961|KLHL17|ENST00000338591|4/12|||207/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 897460 . A C 4476.36 . AC=24;AF=0.2069;AN=116;BaseQRankSum=-6.649;DP=4306;DS;Dels=0.00;FS=1182.881;HRun=6;HaplotypeScore=18.5719;InbreedingCoeff=-0.2955;MQ=53.12;MQ0=0;MQRankSum=-6.585;QD=1.27;ReadPosRankSum=-5.387;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|3),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|4),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 897465 . A C 207.3 . AC=8;AF=0.0678;AN=118;BaseQRankSum=-6.006;DP=4323;DS;Dels=0.00;FS=207.986;HRun=4;HaplotypeScore=15.9325;InbreedingCoeff=-0.0865;MQ=53.13;MQ0=0;MQRankSum=-13.595;QD=0.15;ReadPosRankSum=-11.475;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|3),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|4),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 897470 . A C 60.04 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-10.911;DP=4336;DS;Dels=0.00;FS=267.922;HRun=4;HaplotypeScore=16.3920;InbreedingCoeff=-0.0465;MQ=53.18;MQ0=0;MQRankSum=-11.192;QD=0.22;ReadPosRankSum=-11.844;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|3),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|4),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 897564 . T C 105645.82 . AC=111;AF=0.9407;AN=118;BaseQRankSum=-0.165;DP=3668;DS;Dels=0.00;FS=4.440;HRun=1;HaplotypeScore=1.8075;InbreedingCoeff=0.1915;MQ=54.46;MQ0=0;MQRankSum=3.570;QD=29.65;ReadPosRankSum=-1.056;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|3),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|4),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 897641 . C T 42.61 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.313;DP=2743;DS;Dels=0.00;FS=9.036;HRun=0;HaplotypeScore=1.2616;InbreedingCoeff=-0.0088;MQ=55.91;MQ0=0;MQRankSum=0.432;QD=3.87;ReadPosRankSum=0.049;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|3),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|4),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 897730 . C T 1850.25 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.726;DP=3106;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=3.1479;InbreedingCoeff=-0.0256;MQ=58.41;MQ0=0;MQRankSum=-0.353;QD=12.94;ReadPosRankSum=-0.223;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|3),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|4),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 897738 . C T 5799.05 . AC=8;AF=0.0667;AN=120;BaseQRankSum=1.153;DP=3157;Dels=0.00;FS=6.498;HRun=2;HaplotypeScore=2.6795;InbreedingCoeff=0.1964;MQ=58.52;MQ0=0;MQRankSum=1.044;QD=16.11;ReadPosRankSum=-1.772;EFF=EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|2),SYNONYMOUS_CODING(LOW|SILENT|Ctg/Ttg|L239|642|KLHL17|protein_coding|CODING|ENST00000338591|),SYNONYMOUS_CODING(LOW|SILENT|Ctg/Ttg|L9|412|KLHL17|protein_coding|CODING|ENST00000455747|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,synonymous_variant|Ctg/Ttg|L|ENSG00000187961|KLHL17|ENST00000338591|5/12|||239/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000463212|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 897879 . C T 1100.72 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-0.411;DP=1081;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7665;InbreedingCoeff=-0.0357;MQ=58.62;MQ0=0;MQRankSum=0.745;QD=12.80;ReadPosRankSum=2.064;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|4),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|5),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 898068 . G C 79.28 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.132;DP=1385;Dels=0.00;FS=2.990;HRun=1;HaplotypeScore=0.9318;InbreedingCoeff=0.8872;MQ=57.94;MQ0=0;MQRankSum=0.935;QD=19.82;ReadPosRankSum=-0.735;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|4),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|5),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 898323 . T C 52014.58 . AC=115;AF=0.9583;AN=120;BaseQRankSum=-0.884;DP=1603;Dels=0.00;FS=3.229;HRun=1;HaplotypeScore=0.9054;InbreedingCoeff=-0.0448;MQ=58.52;MQ0=0;MQRankSum=-0.998;QD=32.45;ReadPosRankSum=2.785;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|5),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|6),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=intron_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 898467 . C T 1510.84 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-0.034;DP=1255;Dels=0.00;FS=4.507;HRun=0;HaplotypeScore=0.8357;InbreedingCoeff=-0.0475;MQ=58.85;MQ0=0;MQRankSum=-1.026;QD=13.49;ReadPosRankSum=-1.131;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|5),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|6),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=synonymous_variant&NMD_transcript_variant|ccC/ccT|P|ENSG00000187961|KLHL17|ENST00000466300|2/6|||83/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 898603 . C G 116.84 . AC=8;AF=0.0667;AN=120;BaseQRankSum=-8.523;DP=3456;Dels=0.00;FS=28.932;HRun=2;HaplotypeScore=4.3555;InbreedingCoeff=-0.0746;MQ=57.81;MQ0=0;MQRankSum=-3.388;QD=0.70;ReadPosRankSum=-5.743;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCg/gGg|A156G|412|KLHL17|protein_coding|CODING|ENST00000455747|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCg/gGg|A386G|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=missense_variant&NMD_transcript_variant|Cgg/Ggg|R/G|ENSG00000187961|KLHL17|ENST00000466300|2/6|unknown(0)|deleterious(0)|129/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,missense_variant|gCg/gGg|A/G|ENSG00000187961|KLHL17|ENST00000338591|7/12|possibly_damaging(0.8)|tolerated(0.13)|386/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 898604 . G A 684.72 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.572;DP=3527;Dels=0.00;FS=8.272;HRun=0;HaplotypeScore=4.4131;InbreedingCoeff=-0.0111;MQ=57.83;MQ0=0;MQRankSum=0.825;QD=9.92;ReadPosRankSum=-0.003;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),SYNONYMOUS_CODING(LOW|SILENT|gcG/gcA|A156|412|KLHL17|protein_coding|CODING|ENST00000455747|),SYNONYMOUS_CODING(LOW|SILENT|gcG/gcA|A386|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000477976|);CSQ=missense_variant&NMD_transcript_variant|cGg/cAg|R/Q|ENSG00000187961|KLHL17|ENST00000466300|2/6|unknown(0)|deleterious(0)|129/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,synonymous_variant|gcG/gcA|A|ENSG00000187961|KLHL17|ENST00000338591|7/12|||386/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000477976|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 898893 . G C 345.84 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.109;DP=2327;Dels=0.00;FS=1.347;HRun=0;HaplotypeScore=1.8649;InbreedingCoeff=-0.0084;MQ=58.71;MQ0=0;MQRankSum=0.616;QD=12.81;ReadPosRankSum=0.720;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|7),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|8),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|);CSQ=intron_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,upstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 898934 . G A 47.08 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.111;DP=1477;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6015;InbreedingCoeff=-0.0091;MQ=58.84;MQ0=0;MQRankSum=0.087;QD=5.89;ReadPosRankSum=-1.045;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|1),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|7),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|8),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|);CSQ=intron_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000481067|1/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 899000 . C T 654.34 . AC=8;AF=0.0667;AN=120;BaseQRankSum=-0.932;DP=651;Dels=0.00;FS=1.229;HRun=0;HaplotypeScore=0.5946;InbreedingCoeff=0.3177;MQ=58.35;MQ0=0;MQRankSum=0.454;QD=16.36;ReadPosRankSum=0.885;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|1),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|7),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|8),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|);CSQ=intron_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000481067|1/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 899318 . C T 428.77 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.231;DP=2100;Dels=0.00;FS=29.933;HRun=0;HaplotypeScore=1.6653;InbreedingCoeff=-0.0087;MQ=58.94;MQ0=0;MQRankSum=-0.016;QD=10.99;ReadPosRankSum=-1.614;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|1),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P228|412|KLHL17|protein_coding|CODING|ENST00000455747|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P458|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|);CSQ=3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300|4/6|||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,synonymous_variant|ccC/ccT|P|ENSG00000187961|KLHL17|ENST00000338591|9/12|||458/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000481067|1/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 899374 . G A 298.41 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.204;DP=2588;Dels=0.00;FS=1.169;HRun=1;HaplotypeScore=1.4609;InbreedingCoeff=-0.0084;MQ=58.81;MQ0=0;MQRankSum=0.082;QD=10.29;ReadPosRankSum=2.022;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGa/cAa|R247Q|412|KLHL17|protein_coding|CODING|ENST00000455747|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGa/cAa|R477Q|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|);CSQ=3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300|4/6|||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,missense_variant|cGa/cAa|R/Q|ENSG00000187961|KLHL17|ENST00000338591|9/12|possibly_damaging(0.886)|deleterious(0.03)|477/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000481067|1/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 899515 . C T 554.46 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.826;DP=4376;Dels=0.00;FS=0.766;HRun=0;HaplotypeScore=2.1786;InbreedingCoeff=-0.0084;MQ=58.98;MQ0=0;MQRankSum=-0.199;QD=13.86;ReadPosRankSum=2.919;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),EXON(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|2),SYNONYMOUS_CODING(LOW|SILENT|taC/taT|Y261|412|KLHL17|protein_coding|CODING|ENST00000455747|),SYNONYMOUS_CODING(LOW|SILENT|taC/taT|Y491|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|),UPSTREAM(MODIFIER|||||NOC2L|retained_intron|CODING|ENST00000469563|);CSQ=3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300|5/6|||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,synonymous_variant|taC/taT|Y|ENSG00000187961|KLHL17|ENST00000338591|10/12|||491/642|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000469563|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187961|KLHL17|ENST00000481067|2/2||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 899648 . C T 506.51 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.603;DP=2206;Dels=0.00;FS=1.040;HRun=0;HaplotypeScore=2.2848;InbreedingCoeff=-0.0084;MQ=57.02;MQ0=0;MQRankSum=0.240;QD=14.90;ReadPosRankSum=1.277;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|9),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|10),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||749|NOC2L|protein_coding|CODING|ENST00000327044|),UPSTREAM(MODIFIER|||||NOC2L|processed_transcript|CODING|ENST00000487214|);CSQ=intron_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000327044||||-/749|protein_coding|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,upstream_gene_variant|||ENSG00000188976|NOC2L|ENST00000487214|||||processed_transcript|1 -chr1 899824 . C T 386.57 . AC=2;AF=0.0169;AN=118;BaseQRankSum=-1.360;DP=1027;Dels=0.00;FS=0.938;HRun=0;HaplotypeScore=0.7940;InbreedingCoeff=-0.0327;MQ=52.55;MQ0=0;MQRankSum=2.078;QD=10.17;ReadPosRankSum=-2.959;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N308|412|KLHL17|protein_coding|CODING|ENST00000455747|),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N538|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000187961|KLHL17|ENST00000466300|6/6|||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,synonymous_variant|aaC/aaT|N|ENSG00000187961|KLHL17|ENST00000338591|11/12|||538/642|protein_coding|1 -chr1 899926 . GC G 146.78 . AC=3;AF=0.0273;AN=110;BaseQRankSum=-1.149;DP=401;FS=8.501;HRun=1;HaplotypeScore=98.5276;InbreedingCoeff=-0.0768;MQ=46.11;MQ0=0;MQRankSum=-4.025;QD=4.32;ReadPosRankSum=-1.794;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 899928 . G C 10304.35 . AC=95;AF=0.8636;AN=110;BaseQRankSum=-2.647;DP=394;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.9435;InbreedingCoeff=-0.0052;MQ=45.90;MQ0=0;MQRankSum=-1.207;QD=26.35;ReadPosRankSum=1.974;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 899937 . G T 7323.3 . AC=86;AF=0.860;AN=100;BaseQRankSum=-1.886;DP=301;Dels=0.00;FS=4.882;HRun=0;HaplotypeScore=1.0717;InbreedingCoeff=0.2201;MQ=45.04;MQ0=0;MQRankSum=-1.460;QD=25.61;ReadPosRankSum=2.493;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 899938 . G C 7723.52 . AC=86;AF=0.860;AN=100;BaseQRankSum=-1.269;DP=292;Dels=0.00;FS=6.709;HRun=1;HaplotypeScore=0.9273;InbreedingCoeff=0.2346;MQ=45.14;MQ0=0;MQRankSum=-2.046;QD=27.78;ReadPosRankSum=2.689;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 899942 . G A 9223.42 . AC=87;AF=0.8529;AN=102;BaseQRankSum=-0.795;DP=333;Dels=0.00;FS=4.035;HRun=0;HaplotypeScore=0.7409;InbreedingCoeff=0.1074;MQ=43.28;MQ0=0;MQRankSum=-1.361;QD=28.38;ReadPosRankSum=1.851;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 899943 . G C 99.41 . AC=3;AF=0.0294;AN=102;BaseQRankSum=-1.668;DP=334;Dels=0.00;FS=4.946;HRun=0;HaplotypeScore=0.6513;InbreedingCoeff=-0.0759;MQ=42.96;MQ0=0;MQRankSum=-3.741;QD=2.76;ReadPosRankSum=-1.346;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 899959 . G GC 686.45 . AC=19;AF=0.190;AN=100;BaseQRankSum=-3.215;DP=298;FS=12.605;HRun=0;HaplotypeScore=100.2656;InbreedingCoeff=-0.1846;MQ=45.28;MQ0=0;MQRankSum=-5.322;QD=4.40;ReadPosRankSum=0.742;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 899960 . T C 416.06 . AC=19;AF=0.190;AN=100;BaseQRankSum=3.474;DP=298;Dels=0.00;FS=12.762;HRun=0;HaplotypeScore=0.3615;InbreedingCoeff=-0.1710;MQ=45.28;MQ0=0;MQRankSum=-6.659;QD=2.87;ReadPosRankSum=-1.194;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 899974 . G A 175.25 . AC=17;AF=0.170;AN=100;BaseQRankSum=1.366;DP=246;Dels=0.00;FS=8.615;HRun=0;HaplotypeScore=0.2560;InbreedingCoeff=-0.1500;MQ=45.05;MQ0=0;MQRankSum=-5.035;QD=1.79;ReadPosRankSum=-2.296;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 899975 . C G 161.84 . AC=18;AF=0.180;AN=100;BaseQRankSum=1.074;DP=239;Dels=0.00;FS=8.632;HRun=1;HaplotypeScore=0.2414;InbreedingCoeff=-0.1746;MQ=44.67;MQ0=0;MQRankSum=-6.140;QD=1.54;ReadPosRankSum=-2.132;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 899987 . GGATGT G 805.24 . AC=10;AF=0.102;AN=98;BaseQRankSum=-2.007;DP=183;FS=4.301;HRun=0;HaplotypeScore=71.0169;InbreedingCoeff=0.1259;MQ=41.55;MQ0=0;MQRankSum=-2.599;QD=19.64;ReadPosRankSum=-2.063;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 899998 . G C 172.67 . AC=3;AF=0.0288;AN=104;BaseQRankSum=-3.045;DP=165;Dels=0.01;FS=6.898;HRun=1;HaplotypeScore=3.2446;InbreedingCoeff=-0.1274;MQ=38.58;MQ0=0;MQRankSum=-2.770;QD=7.85;ReadPosRankSum=2.697;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900000 . G GAA 4516.13 . AC=82;AF=0.8039;AN=102;BaseQRankSum=-0.011;DP=158;FS=8.933;HRun=0;HaplotypeScore=53.7512;InbreedingCoeff=0.0958;MQ=37.58;MQ0=0;MQRankSum=-2.778;QD=29.91;ReadPosRankSum=0.180;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900001 . G A 1769.07 . AC=55;AF=0.561;AN=98;BaseQRankSum=-1.838;DP=158;Dels=0.00;FS=12.659;HRun=1;HaplotypeScore=4.3165;InbreedingCoeff=0.0703;MQ=37.58;MQ0=0;MQRankSum=-1.578;QD=15.38;ReadPosRankSum=-0.873;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900009 . C G 1460.59 . AC=51;AF=0.510;AN=100;BaseQRankSum=1.557;DP=154;Dels=0.00;FS=5.254;HRun=6;HaplotypeScore=3.7869;InbreedingCoeff=0.1584;MQ=37.06;MQ0=0;MQRankSum=-2.397;QD=14.75;ReadPosRankSum=1.027;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900009 . CG C 3423.69 . AC=75;AF=0.765;AN=98;BaseQRankSum=-1.591;DP=149;FS=3.362;HRun=6;HaplotypeScore=53.0225;InbreedingCoeff=-0.0140;MQ=37.05;MQ0=0;MQRankSum=0.377;QD=24.45;ReadPosRankSum=3.509;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900010 . G GC 2274.92 . AC=67;AF=0.684;AN=98;BaseQRankSum=-2.660;DP=149;FS=0.000;HRun=1;HaplotypeScore=58.6576;InbreedingCoeff=0.1712;MQ=37.05;MQ0=0;MQRankSum=0.765;QD=18.35;ReadPosRankSum=2.099;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900012 . G A 52.89 . AC=9;AF=0.092;AN=98;BaseQRankSum=-1.584;DP=144;Dels=0.00;FS=9.518;HRun=0;HaplotypeScore=1.9499;InbreedingCoeff=-0.1191;MQ=37.30;MQ0=0;MQRankSum=-1.274;QD=1.56;ReadPosRankSum=-3.899;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900018 . G A 202.57 . AC=3;AF=0.033;AN=90;BaseQRankSum=-0.005;DP=138;Dels=0.00;FS=14.921;HRun=0;HaplotypeScore=2.7642;InbreedingCoeff=-0.1390;MQ=37.35;MQ0=0;MQRankSum=-2.631;QD=11.25;ReadPosRankSum=-0.202;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900025 . GGATGT G 524.76 . AC=16;AF=0.174;AN=92;BaseQRankSum=3.838;DP=133;FS=8.461;HRun=0;HaplotypeScore=57.1337;InbreedingCoeff=-0.0789;MQ=37.07;MQ0=0;MQRankSum=-2.025;QD=12.49;ReadPosRankSum=0.267;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900036 . C G 56.0 . AC=8;AF=0.095;AN=84;BaseQRankSum=-0.360;DP=115;Dels=0.02;FS=4.181;HRun=3;HaplotypeScore=2.4570;InbreedingCoeff=0.0759;MQ=37.96;MQ0=0;MQRankSum=-2.183;QD=3.73;ReadPosRankSum=1.383;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900045 . G T 197.92 . AC=12;AF=0.158;AN=76;BaseQRankSum=-0.033;DP=97;Dels=0.00;FS=9.602;HRun=0;HaplotypeScore=0.8494;InbreedingCoeff=-0.1283;MQ=36.39;MQ0=0;MQRankSum=-3.050;QD=6.60;ReadPosRankSum=0.794;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900046 . G C 221.18 . AC=14;AF=0.194;AN=72;BaseQRankSum=0.606;DP=95;Dels=0.00;FS=15.119;HRun=1;HaplotypeScore=0.8152;InbreedingCoeff=-0.0844;MQ=36.53;MQ0=0;MQRankSum=-3.254;QD=6.91;ReadPosRankSum=0.931;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900047 . CG C 209.92 . AC=15;AF=0.208;AN=72;BaseQRankSum=1.707;DP=88;FS=0.000;HRun=6;HaplotypeScore=49.2935;InbreedingCoeff=-0.1925;MQ=36.21;MQ0=0;MQRankSum=-2.511;QD=4.47;ReadPosRankSum=0.230;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900050 . G A 240.82 . AC=14;AF=0.226;AN=62;BaseQRankSum=1.097;DP=84;Dels=0.00;FS=15.435;HRun=0;HaplotypeScore=0.6581;InbreedingCoeff=-0.0898;MQ=34.67;MQ0=0;MQRankSum=-1.036;QD=8.03;ReadPosRankSum=0.391;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900065 . A C 247.12 . AC=17;AF=0.304;AN=56;BaseQRankSum=1.516;DP=63;Dels=0.00;FS=9.830;HRun=0;HaplotypeScore=2.1490;InbreedingCoeff=-0.0549;MQ=32.56;MQ0=0;MQRankSum=-0.918;QD=7.06;ReadPosRankSum=2.224;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900068 . TGCTGCC T 518.39 . AC=13;AF=0.217;AN=60;BaseQRankSum=4.713;DP=59;FS=14.526;HRun=0;HaplotypeScore=44.2811;InbreedingCoeff=0.0509;MQ=31.33;MQ0=0;MQRankSum=-0.386;QD=28.80;ReadPosRankSum=4.120;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900083 . G T 196.8 . AC=12;AF=0.286;AN=42;BaseQRankSum=-2.303;DP=46;Dels=0.00;FS=9.918;HRun=0;HaplotypeScore=1.2000;InbreedingCoeff=0.0257;MQ=29.00;MQ0=0;MQRankSum=0.317;QD=10.36;ReadPosRankSum=1.627;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900084 . G C 236.51 . AC=14;AF=0.350;AN=40;BaseQRankSum=-0.904;DP=46;Dels=0.00;FS=12.035;HRun=1;HaplotypeScore=1.2233;InbreedingCoeff=0.0591;MQ=29.00;MQ0=0;MQRankSum=-0.215;QD=11.83;ReadPosRankSum=1.420;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900085 . C G 54.45 . AC=7;AF=0.175;AN=40;BaseQRankSum=1.312;DP=45;Dels=0.00;FS=0.000;HRun=6;HaplotypeScore=0.9242;InbreedingCoeff=0.1696;MQ=29.00;MQ0=0;MQRankSum=0.641;QD=7.78;ReadPosRankSum=-1.373;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900086 . G GC 66.18 . AC=9;AF=0.265;AN=34;BaseQRankSum=-0.405;DP=42;FS=3.909;HRun=1;HaplotypeScore=46.6346;InbreedingCoeff=0.0729;MQ=29.00;MQ0=0;MQRankSum=0.231;QD=4.41;ReadPosRankSum=-0.925;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900089 . G C 230.72 . AC=14;AF=0.500;AN=28;BaseQRankSum=0.692;DP=39;Dels=0.00;FS=10.780;HRun=0;HaplotypeScore=0.8484;InbreedingCoeff=0.2201;MQ=29.00;MQ0=0;MQRankSum=0.165;QD=14.42;ReadPosRankSum=0.495;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900103 . A C 245.84 . AC=13;AF=0.650;AN=20;BaseQRankSum=2.098;DP=29;Dels=0.00;FS=4.393;HRun=0;HaplotypeScore=1.1682;InbreedingCoeff=-0.0533;MQ=29.00;MQ0=0;MQRankSum=2.098;QD=15.37;ReadPosRankSum=0.146;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900112 . C G 149.58 . AC=10;AF=0.556;AN=18;BaseQRankSum=1.027;DP=24;Dels=0.04;FS=2.276;HRun=3;HaplotypeScore=0.9865;MQ=29.00;MQ0=0;MQRankSum=1.027;QD=12.47;ReadPosRankSum=0.201;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900115 . G A 35.09 . AC=5;AF=0.227;AN=22;BaseQRankSum=2.382;DP=23;Dels=0.00;FS=13.570;HRun=1;HaplotypeScore=1.7631;InbreedingCoeff=0.1307;MQ=29.00;MQ0=0;MQRankSum=-0.361;QD=7.02;ReadPosRankSum=-1.083;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900123 . C G 41.39 . AC=5;AF=0.357;AN=14;BaseQRankSum=1.931;DP=20;Dels=0.00;FS=14.771;HRun=6;HaplotypeScore=1.5461;MQ=29.00;MQ0=0;MQRankSum=0.000;QD=5.17;ReadPosRankSum=-0.382;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900123 . CG C 239.86 . AC=12;AF=0.600;AN=20;BaseQRankSum=0.715;DP=20;FS=2.363;HRun=6;HaplotypeScore=37.1615;InbreedingCoeff=0.0592;MQ=29.00;MQ0=0;MQRankSum=-0.965;QD=17.13;ReadPosRankSum=0.627;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900161 . C G 95.96 . AC=3;AF=0.041;AN=74;BaseQRankSum=-0.808;DP=152;Dels=0.00;FS=0.000;HRun=6;HaplotypeScore=0.5011;InbreedingCoeff=-0.1315;MQ=55.56;MQ0=0;MQRankSum=1.024;QD=4.17;ReadPosRankSum=-2.738;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900188 . C G 109.47 . AC=2;AF=0.021;AN=96;BaseQRankSum=0.425;DP=319;Dels=0.00;FS=0.000;HRun=3;HaplotypeScore=0.5571;InbreedingCoeff=-0.0765;MQ=55.58;MQ0=0;MQRankSum=-4.308;QD=9.95;ReadPosRankSum=-1.562;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900191 . G A 78.75 . AC=2;AF=0.020;AN=98;BaseQRankSum=0.169;DP=359;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.6619;InbreedingCoeff=-0.0713;MQ=55.88;MQ0=0;MQRankSum=-3.973;QD=7.16;ReadPosRankSum=-0.841;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900199 . C G 122.69 . AC=2;AF=0.0196;AN=102;BaseQRankSum=-0.625;DP=419;Dels=0.00;FS=5.991;HRun=6;HaplotypeScore=0.9712;InbreedingCoeff=-0.0705;MQ=55.82;MQ0=0;MQRankSum=-4.226;QD=11.15;ReadPosRankSum=-0.138;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900200 . G GC 138.36 . AC=2;AF=0.0192;AN=104;BaseQRankSum=4.004;DP=447;FS=5.745;HRun=1;HaplotypeScore=63.8129;InbreedingCoeff=-0.0683;MQ=55.87;MQ0=0;MQRankSum=-4.054;QD=10.64;ReadPosRankSum=-0.688;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900205 . T C 3848.55 . AC=28;AF=0.2642;AN=106;BaseQRankSum=3.976;DP=500;Dels=0.00;FS=4.311;HRun=2;HaplotypeScore=0.8450;InbreedingCoeff=-0.0127;MQ=56.04;MQ0=0;MQRankSum=2.371;QD=15.21;ReadPosRankSum=0.624;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900208 . G A 196.8 . AC=3;AF=0.0278;AN=108;BaseQRankSum=-1.850;DP=545;Dels=0.00;FS=3.034;HRun=0;HaplotypeScore=0.9217;InbreedingCoeff=-0.0711;MQ=55.89;MQ0=0;MQRankSum=-1.612;QD=8.56;ReadPosRankSum=-1.195;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900252 . G A 2492.99 . AC=7;AF=0.0593;AN=118;BaseQRankSum=0.859;DP=1112;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.6311;InbreedingCoeff=-0.0857;MQ=55.70;MQ0=0;MQRankSum=2.953;QD=13.33;ReadPosRankSum=-0.555;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900285 . C T 49972.75 . AC=105;AF=0.9052;AN=116;BaseQRankSum=-3.461;DP=1713;Dels=0.00;FS=2.048;HRun=0;HaplotypeScore=2.6694;InbreedingCoeff=0.0387;MQ=55.27;MQ0=0;MQRankSum=-1.138;QD=29.80;ReadPosRankSum=-0.012;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900286 . A G 53795.91 . AC=111;AF=0.9569;AN=116;BaseQRankSum=-1.271;DP=1744;Dels=0.00;FS=6.893;HRun=1;HaplotypeScore=2.7132;InbreedingCoeff=-0.0679;MQ=55.34;MQ0=0;MQRankSum=0.681;QD=30.85;ReadPosRankSum=0.607;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900298 . C G 1312.84 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-2.430;DP=2069;Dels=0.00;FS=2.014;HRun=1;HaplotypeScore=1.8253;InbreedingCoeff=0.1410;MQ=55.23;MQ0=0;MQRankSum=1.938;QD=10.67;ReadPosRankSum=-0.720;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900319 . G A 830.69 . AC=3;AF=0.0250;AN=120;BaseQRankSum=0.864;DP=2658;Dels=0.00;FS=5.182;HRun=0;HaplotypeScore=1.8883;InbreedingCoeff=-0.0417;MQ=56.04;MQ0=0;MQRankSum=2.734;QD=10.93;ReadPosRankSum=1.705;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|10),INTRON(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|11),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 900397 . C T 2009.84 . AC=2;AF=0.0167;AN=120;BaseQRankSum=1.145;DP=5436;DS;Dels=0.00;FS=0.454;HRun=0;HaplotypeScore=4.5718;InbreedingCoeff=-0.0195;MQ=57.26;MQ0=0;MQRankSum=1.619;QD=12.72;ReadPosRankSum=-0.157;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N355|412|KLHL17|protein_coding|CODING|ENST00000455747|),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N585|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,synonymous_variant|aaC/aaT|N|ENSG00000187961|KLHL17|ENST00000338591|12/12|||585/642|protein_coding|1 -chr1 900505 . G C 36708.31 . AC=34;AF=0.2881;AN=118;BaseQRankSum=3.779;DP=6012;DS;Dels=0.00;FS=16.118;HRun=1;HaplotypeScore=4.6415;InbreedingCoeff=-0.1592;MQ=56.94;MQ0=0;MQRankSum=2.324;QD=13.04;ReadPosRankSum=-1.518;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),SYNONYMOUS_CODING(LOW|SILENT|gtG/gtC|V391|412|KLHL17|protein_coding|CODING|ENST00000455747|),SYNONYMOUS_CODING(LOW|SILENT|gtG/gtC|V621|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,synonymous_variant|gtG/gtC|V|ENSG00000187961|KLHL17|ENST00000338591|12/12|||621/642|protein_coding|1 -chr1 900560 . A C 336.55 . AC=11;AF=0.0917;AN=120;BaseQRankSum=-16.919;DP=3961;DS;Dels=0.00;FS=94.082;HRun=2;HaplotypeScore=2.4690;InbreedingCoeff=-0.1027;MQ=56.02;MQ0=0;MQRankSum=-0.133;QD=2.37;ReadPosRankSum=-4.673;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Acc/Ccc|T410P|412|KLHL17|protein_coding|CODING|ENST00000455747|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Acc/Ccc|T640P|642|KLHL17|protein_coding|CODING|ENST00000338591|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,missense_variant|Acc/Ccc|T/P|ENSG00000187961|KLHL17|ENST00000338591|12/12|unknown(0)|deleterious(0.02)|640/642|protein_coding|1 -chr1 900717 . CTTAT C 9074.2 . AC=69;AF=0.6053;AN=114;BaseQRankSum=10.277;DP=412;FS=7.468;HRun=0;HaplotypeScore=48.3915;InbreedingCoeff=0.0298;MQ=48.25;MQ0=0;MQRankSum=-9.614;QD=26.85;ReadPosRankSum=3.811;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UTR_3_PRIME(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|11),UTR_3_PRIME(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|12);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,3_prime_UTR_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591|12/12|||-/642|protein_coding|1 -chr1 900723 . T G 285.98 . AC=14;AF=0.1228;AN=114;BaseQRankSum=-3.773;DP=321;Dels=0.00;FS=2.147;HRun=0;HaplotypeScore=11.3195;InbreedingCoeff=-0.1788;MQ=46.06;MQ0=0;MQRankSum=4.705;QD=2.95;ReadPosRankSum=-6.059;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UTR_3_PRIME(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|11),UTR_3_PRIME(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|12);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187961|KLHL17|ENST00000338591|12/12|||-/642|protein_coding|1 -chr1 900730 . G A 7842.1 . AC=100;AF=0.8621;AN=116;BaseQRankSum=-0.675;DP=275;Dels=0.00;FS=1.534;HRun=0;HaplotypeScore=5.4770;InbreedingCoeff=0.2758;MQ=43.76;MQ0=0;MQRankSum=-4.770;QD=29.93;ReadPosRankSum=-1.794;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UTR_3_PRIME(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|11),UTR_3_PRIME(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|12);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187961|KLHL17|ENST00000338591|12/12|||-/642|protein_coding|1 -chr1 900972 . T G 837.46 . AC=38;AF=0.950;AN=40;BaseQRankSum=1.091;DP=28;Dels=0.00;FS=5.863;HRun=1;HaplotypeScore=0.0000;InbreedingCoeff=-0.0011;MQ=56.62;MQ0=0;MQRankSum=1.091;QD=32.21;ReadPosRankSum=1.348;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UTR_3_PRIME(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|11),UTR_3_PRIME(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|12);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187961|KLHL17|ENST00000338591|12/12|||-/642|protein_coding|1 -chr1 901023 . T C 668.0 . AC=30;AF=0.882;AN=34;BaseQRankSum=0.039;DP=25;Dels=0.00;FS=13.318;HRun=0;HaplotypeScore=0.1562;InbreedingCoeff=-0.0502;MQ=59.07;MQ0=0;MQRankSum=-1.123;QD=29.04;ReadPosRankSum=-2.053;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UTR_3_PRIME(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|11),UTR_3_PRIME(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|12);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187961|KLHL17|ENST00000338591|12/12|||-/642|protein_coding|1 -chr1 901046 . ACC A 367.24 . AC=14;AF=0.368;AN=38;BaseQRankSum=3.349;DP=34;FS=3.319;HRun=4;HaplotypeScore=8.5629;InbreedingCoeff=0.2418;MQ=57.94;MQ0=0;MQRankSum=-0.562;QD=26.23;ReadPosRankSum=0.832;EFF=DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UTR_3_PRIME(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|11),UTR_3_PRIME(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|12);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,3_prime_UTR_variant&feature_truncation|||ENSG00000187961|KLHL17|ENST00000338591|12/12|||-/642|protein_coding|1 -chr1 901460 . C T 32.18 . AC=2;AF=0.045;AN=44;BaseQRankSum=0.046;DP=29;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0879;InbreedingCoeff=-0.0076;MQ=59.20;MQ0=0;MQRankSum=0.231;QD=16.09;ReadPosRankSum=-1.435;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 901559 . G A 1182.94 . AC=46;AF=0.920;AN=50;BaseQRankSum=0.433;DP=38;Dels=0.00;FS=2.501;HRun=0;HaplotypeScore=0.1286;InbreedingCoeff=-0.0789;MQ=56.89;MQ0=0;MQRankSum=-0.974;QD=31.97;ReadPosRankSum=-0.704;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 901607 . C G 1775.67 . AC=57;AF=0.983;AN=58;BaseQRankSum=-0.817;DP=56;Dels=0.00;FS=0.000;HRun=6;HaplotypeScore=0.0000;InbreedingCoeff=-0.1683;MQ=56.62;MQ0=0;MQRankSum=-0.443;QD=32.28;ReadPosRankSum=-0.303;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 901652 . A G 2979.53 . AC=72;AF=1.000;AN=72;DP=87;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0518;InbreedingCoeff=-0.1163;MQ=57.30;MQ0=0;QD=34.65;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 901806 . G C 1993.1 . AC=13;AF=0.1204;AN=108;BaseQRankSum=0.578;DP=624;Dels=0.00;FS=2.492;HRun=3;HaplotypeScore=0.2501;InbreedingCoeff=0.2481;MQ=58.77;MQ0=0;MQRankSum=1.452;QD=19.16;ReadPosRankSum=0.786;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|),UPSTREAM(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|),UPSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 901919 . A C 43.36 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-7.616;DP=1450;Dels=0.00;FS=55.854;HRun=1;HaplotypeScore=0.9087;InbreedingCoeff=-0.0627;MQ=58.76;MQ0=0;MQRankSum=-1.305;QD=0.46;ReadPosRankSum=-4.128;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAc/aCc|N3T|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAc/aCc|N3T|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aAc/aCc|N3T|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,missense_variant|aAc/aCc|N/T|ENSG00000187583|PLEKHN1|ENST00000379409|1/15|possibly_damaging(0.471)|deleterious(0.01)|3/663|protein_coding|1,missense_variant|aAc/aCc|N/T|ENSG00000187583|PLEKHN1|ENST00000379407|1/15|benign(0.331)|deleterious(0.01)|3/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,missense_variant|aAc/aCc|N/T|ENSG00000187583|PLEKHN1|ENST00000379410|1/16|benign(0.444)|deleterious(0.01)|3/611|protein_coding|1 -chr1 902043 . G A 169.05 . AC=2;AF=0.0172;AN=116;BaseQRankSum=-1.369;DP=777;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.0432;InbreedingCoeff=-0.0431;MQ=58.28;MQ0=0;MQRankSum=0.826;QD=5.45;ReadPosRankSum=-0.446;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|1),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|1),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|1),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 902069 . T C 177.69 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.199;DP=717;Dels=0.00;FS=3.490;HRun=4;HaplotypeScore=0.5354;InbreedingCoeff=-0.0340;MQ=58.23;MQ0=0;MQRankSum=0.502;QD=7.11;ReadPosRankSum=0.208;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|1),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|1),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|1),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 902108 . C T 116.44 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.766;DP=558;Dels=0.00;FS=3.848;HRun=0;HaplotypeScore=0.5318;InbreedingCoeff=-0.0325;MQ=58.52;MQ0=0;MQRankSum=-0.417;QD=8.96;ReadPosRankSum=0.186;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A36|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A36|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A36|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,synonymous_variant|gcC/gcT|A|ENSG00000187583|PLEKHN1|ENST00000379409|2/15|||36/663|protein_coding|1,synonymous_variant|gcC/gcT|A|ENSG00000187583|PLEKHN1|ENST00000379407|2/15|||36/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,synonymous_variant|gcC/gcT|A|ENSG00000187583|PLEKHN1|ENST00000379410|2/16|||36/611|protein_coding|1 -chr1 902128 . C T 375.73 . AC=3;AF=0.0254;AN=118;BaseQRankSum=-0.165;DP=545;Dels=0.00;FS=0.993;HRun=1;HaplotypeScore=0.3151;InbreedingCoeff=-0.0508;MQ=58.23;MQ0=0;MQRankSum=0.962;QD=11.39;ReadPosRankSum=-1.116;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A43V|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A43V|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCt/gTt|A43V|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,missense_variant|gCt/gTt|A/V|ENSG00000187583|PLEKHN1|ENST00000379409|2/15|possibly_damaging(0.586)|deleterious(0.01)|43/663|protein_coding|1,missense_variant|gCt/gTt|A/V|ENSG00000187583|PLEKHN1|ENST00000379407|2/15|benign(0.209)|deleterious(0.01)|43/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,missense_variant|gCt/gTt|A/V|ENSG00000187583|PLEKHN1|ENST00000379410|2/16|benign(0.209)|deleterious(0.01)|43/611|protein_coding|1 -chr1 902288 . G A 297.6 . AC=8;AF=0.083;AN=96;BaseQRankSum=-1.235;DP=169;Dels=0.00;FS=6.677;HRun=0;HaplotypeScore=0.1735;InbreedingCoeff=0.2484;MQ=58.34;MQ0=0;MQRankSum=1.682;QD=12.94;ReadPosRankSum=-1.820;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000463212|),DOWNSTREAM(MODIFIER|||||KLHL17|retained_intron|CODING|ENST00000481067|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|2),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|2),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|2),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000466300||||-/154|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000463212|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000481067|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 905130 . ATG A 1155.32 . AC=23;AF=0.442;AN=52;BaseQRankSum=1.417;DP=60;FS=3.885;HRun=0;HaplotypeScore=17.3266;InbreedingCoeff=0.3250;MQ=54.35;MQ0=0;MQRankSum=-1.757;QD=30.40;ReadPosRankSum=1.061;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|2),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|2),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|2),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 905158 . TGC T 181.75 . AC=10;AF=0.200;AN=50;BaseQRankSum=-3.797;DP=55;FS=1.587;HRun=0;HaplotypeScore=23.4217;InbreedingCoeff=0.1863;MQ=54.38;MQ0=0;MQRankSum=0.194;QD=12.98;ReadPosRankSum=-2.056;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|2),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|2),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|2),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 905165 . A G 1906.87 . AC=48;AF=0.960;AN=50;BaseQRankSum=0.088;DP=59;Dels=0.00;FS=5.156;HRun=0;HaplotypeScore=0.3866;InbreedingCoeff=0.0421;MQ=56.05;MQ0=0;MQRankSum=1.086;QD=32.88;ReadPosRankSum=-1.380;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|2),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|2),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|2),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 905894 . C T 1520.95 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-2.494;DP=3276;Dels=0.00;FS=2.372;HRun=0;HaplotypeScore=1.4299;InbreedingCoeff=-0.0172;MQ=58.58;MQ0=0;MQRankSum=0.861;QD=13.46;ReadPosRankSum=-0.683;EFF=DOWNSTREAM(MODIFIER||||412|KLHL17|protein_coding|CODING|ENST00000455747|),DOWNSTREAM(MODIFIER||||642|KLHL17|protein_coding|CODING|ENST00000338591|),DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|3),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|3),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|3),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|),UPSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1,downstream_gene_variant|||ENSG00000187961|KLHL17|ENST00000338591||||-/642|protein_coding|1 -chr1 906272 . A C 2509.21 . AC=19;AF=0.1583;AN=120;BaseQRankSum=3.923;DP=937;Dels=0.00;FS=33.565;HRun=4;HaplotypeScore=0.7062;InbreedingCoeff=0.0234;MQ=58.16;MQ0=0;MQRankSum=0.166;QD=10.72;ReadPosRankSum=0.823;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),EXON(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|1),SYNONYMOUS_CODING(LOW|SILENT|gcA/gcC|A166|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|gcA/gcC|A166|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|gcA/gcC|A206|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,synonymous_variant|gcA/gcC|A|ENSG00000187583|PLEKHN1|ENST00000379409|5/15|||206/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|1/3||||retained_intron|1,synonymous_variant|gcA/gcC|A|ENSG00000187583|PLEKHN1|ENST00000379407|6/15|||166/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|gcA/gcC|A|ENSG00000187583|PLEKHN1|ENST00000379410|6/16|||166/611|protein_coding|1 -chr1 906302 . C T 240.96 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-1.006;DP=838;Dels=0.00;FS=7.521;HRun=0;HaplotypeScore=0.4687;InbreedingCoeff=-0.0191;MQ=58.08;MQ0=0;MQRankSum=-0.315;QD=8.03;ReadPosRankSum=-2.447;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),EXON(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|1),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A176|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A176|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A216|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,synonymous_variant|gcC/gcT|A|ENSG00000187583|PLEKHN1|ENST00000379409|5/15|||216/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|1/3||||retained_intron|1,synonymous_variant|gcC/gcT|A|ENSG00000187583|PLEKHN1|ENST00000379407|6/15|||176/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|gcC/gcT|A|ENSG00000187583|PLEKHN1|ENST00000379410|6/16|||176/611|protein_coding|1 -chr1 906850 . C T 840.9 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.291;DP=1812;Dels=0.00;FS=2.912;HRun=0;HaplotypeScore=1.3291;InbreedingCoeff=-0.0167;MQ=58.39;MQ0=0;MQRankSum=0.375;QD=15.02;ReadPosRankSum=0.230;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),EXON(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|3),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|8),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|8),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|7),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|3/3||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 906872 . C T 490.99 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.837;DP=1326;Dels=0.00;FS=11.727;HRun=3;HaplotypeScore=0.8532;InbreedingCoeff=-0.0185;MQ=58.72;MQ0=0;MQRankSum=0.435;QD=14.88;ReadPosRankSum=0.427;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),EXON(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|3),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|8),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|8),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|7),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|3/3||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 906974 . G A 150.57 . AC=2;AF=0.0185;AN=108;BaseQRankSum=0.388;DP=247;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1707;InbreedingCoeff=-0.0985;MQ=58.20;MQ0=0;MQRankSum=0.405;QD=12.55;ReadPosRankSum=0.062;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|8),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|8),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|7),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 907023 . G A 49.9 . AC=4;AF=0.048;AN=84;BaseQRankSum=0.466;DP=145;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1426;InbreedingCoeff=0.2013;MQ=59.04;MQ0=0;MQRankSum=0.872;QD=16.63;ReadPosRankSum=-0.015;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|8),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|8),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|7),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 907170 . AG A 1280.62 . AC=36;AF=0.3462;AN=104;BaseQRankSum=3.258;DP=132;FS=0.000;HRun=3;HaplotypeScore=8.3225;InbreedingCoeff=0.2957;MQ=57.69;MQ0=0;MQRankSum=0.182;QD=20.99;ReadPosRankSum=0.423;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|8),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|8),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|7),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 907609 . T C 2023.79 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-2.518;DP=2587;Dels=0.00;FS=0.956;HRun=0;HaplotypeScore=2.5448;InbreedingCoeff=-0.0366;MQ=48.44;MQ0=0;MQRankSum=4.469;QD=10.76;ReadPosRankSum=2.738;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|9),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|9),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|8),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 907622 . G C 3088.17 . AC=7;AF=0.0583;AN=120;BaseQRankSum=-1.813;DP=2382;Dels=0.00;FS=1.696;HRun=0;HaplotypeScore=13.8552;InbreedingCoeff=0.2112;MQ=46.67;MQ0=0;MQRankSum=5.413;QD=12.21;ReadPosRankSum=2.166;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|9),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|9),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|8),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 907631 . A C 2295.85 . AC=35;AF=0.2917;AN=120;BaseQRankSum=-20.997;DP=2266;Dels=0.00;FS=665.053;HRun=7;HaplotypeScore=7.6604;InbreedingCoeff=-0.4342;MQ=46.08;MQ0=0;MQRankSum=-14.611;QD=1.46;ReadPosRankSum=13.032;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|9),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|9),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|8),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 907758 . A G 191.93 . AC=13;AF=0.1140;AN=114;BaseQRankSum=-12.237;DP=1481;Dels=0.01;FS=153.003;HRun=5;HaplotypeScore=5.2587;InbreedingCoeff=-0.1585;MQ=51.66;MQ0=0;MQRankSum=-11.759;QD=0.46;ReadPosRankSum=1.329;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAg/gGg|E319G|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAg/gGg|E331G|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAg/gGg|E371G|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,missense_variant|gAg/gGg|E/G|ENSG00000187583|PLEKHN1|ENST00000379409|9/15|benign(0.005)|tolerated(0.28)|371/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,missense_variant|gAg/gGg|E/G|ENSG00000187583|PLEKHN1|ENST00000379407|10/15|benign(0.001)|tolerated(0.21)|331/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,missense_variant|gAg/gGg|E/G|ENSG00000187583|PLEKHN1|ENST00000379410|10/16|benign(0.003)|tolerated(0.26)|319/611|protein_coding|1 -chr1 907774 . G A 268.81 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.645;DP=1416;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.8695;InbreedingCoeff=-0.0177;MQ=51.53;MQ0=0;MQRankSum=0.725;QD=7.47;ReadPosRankSum=-2.464;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),SYNONYMOUS_CODING(LOW|SILENT|ctG/ctA|L324|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|ctG/ctA|L336|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|ctG/ctA|L376|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,synonymous_variant|ctG/ctA|L|ENSG00000187583|PLEKHN1|ENST00000379409|9/15|||376/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,synonymous_variant|ctG/ctA|L|ENSG00000187583|PLEKHN1|ENST00000379407|10/15|||336/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|ctG/ctA|L|ENSG00000187583|PLEKHN1|ENST00000379410|10/16|||324/611|protein_coding|1 -chr1 907816 . A G 331.68 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.634;DP=1030;Dels=0.00;FS=2.656;HRun=0;HaplotypeScore=0.8456;InbreedingCoeff=-0.0346;MQ=55.40;MQ0=0;MQRankSum=0.355;QD=10.05;ReadPosRankSum=3.222;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|10),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|10),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|9),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 907920 . C G 216.19 . AC=4;AF=0.0377;AN=106;BaseQRankSum=0.780;DP=253;Dels=0.00;FS=0.000;HRun=3;HaplotypeScore=0.2690;InbreedingCoeff=0.1203;MQ=58.25;MQ0=0;MQRankSum=-0.123;QD=16.63;ReadPosRankSum=-0.212;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|10),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|10),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|9),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 907991 . CA C 106.79 . AC=5;AF=0.054;AN=92;BaseQRankSum=4.179;DP=101;FS=1.708;HRun=7;HaplotypeScore=8.2099;InbreedingCoeff=0.0500;MQ=56.72;MQ0=0;MQRankSum=-1.342;QD=11.87;ReadPosRankSum=1.095;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|10),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|10),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|9),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 907994 . A C 162.44 . AC=5;AF=0.054;AN=92;BaseQRankSum=3.562;DP=99;Dels=0.00;FS=3.801;HRun=0;HaplotypeScore=0.0865;InbreedingCoeff=0.0684;MQ=56.41;MQ0=0;MQRankSum=-2.224;QD=16.24;ReadPosRankSum=1.331;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|10),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|10),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|9),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 907998 . A T 165.43 . AC=5;AF=0.053;AN=94;BaseQRankSum=2.279;DP=96;Dels=0.00;FS=1.648;HRun=2;HaplotypeScore=0.0847;InbreedingCoeff=0.0537;MQ=56.29;MQ0=0;MQRankSum=-1.944;QD=18.38;ReadPosRankSum=1.172;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|10),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|10),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|9),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 908170 . C G 938.77 . AC=9;AF=0.0763;AN=118;BaseQRankSum=-2.147;DP=536;Dels=0.00;FS=1.531;HRun=2;HaplotypeScore=0.5568;InbreedingCoeff=0.1081;MQ=53.86;MQ0=0;MQRankSum=0.079;QD=11.31;ReadPosRankSum=0.858;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|10),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|10),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|9),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 908228 . A G 406.65 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.194;DP=1035;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5043;InbreedingCoeff=-0.0201;MQ=55.14;MQ0=0;MQRankSum=1.782;QD=12.32;ReadPosRankSum=-0.055;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|10),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|10),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|9),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 908275 . G A 810.31 . AC=3;AF=0.0250;AN=120;BaseQRankSum=0.050;DP=1417;Dels=0.00;FS=4.591;HRun=0;HaplotypeScore=0.6695;InbreedingCoeff=-0.0308;MQ=55.19;MQ0=0;MQRankSum=1.728;QD=10.39;ReadPosRankSum=0.854;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gAc|G346D|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gAc|G358D|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gGc/gAc|G398D|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,missense_variant|gGc/gAc|G/D|ENSG00000187583|PLEKHN1|ENST00000379409|10/15|benign(0.006)|tolerated(1)|398/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,missense_variant|gGc/gAc|G/D|ENSG00000187583|PLEKHN1|ENST00000379407|11/15|benign(0.006)|tolerated(1)|358/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,missense_variant|gGc/gAc|G/D|ENSG00000187583|PLEKHN1|ENST00000379410|11/16|benign(0.002)|tolerated(1)|346/611|protein_coding|1 -chr1 908325 . A C 267.79 . AC=14;AF=0.1167;AN=120;BaseQRankSum=-17.215;DP=1827;Dels=0.00;FS=693.401;HRun=2;HaplotypeScore=3.2868;InbreedingCoeff=-0.1506;MQ=55.13;MQ0=0;MQRankSum=-1.642;QD=0.79;ReadPosRankSum=0.894;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Acc/Ccc|T363P|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Acc/Ccc|T375P|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Acc/Ccc|T415P|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,missense_variant|Acc/Ccc|T/P|ENSG00000187583|PLEKHN1|ENST00000379409|10/15|probably_damaging(0.998)|tolerated(0.08)|415/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,missense_variant|Acc/Ccc|T/P|ENSG00000187583|PLEKHN1|ENST00000379407|11/15|probably_damaging(0.998)|tolerated(0.09)|375/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,missense_variant|Acc/Ccc|T/P|ENSG00000187583|PLEKHN1|ENST00000379410|11/16|probably_damaging(0.995)|tolerated(0.11)|363/611|protein_coding|1 -chr1 908414 . C T 3063.53 . AC=7;AF=0.0583;AN=120;BaseQRankSum=-1.682;DP=2413;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.8594;InbreedingCoeff=-0.0636;MQ=57.64;MQ0=0;MQRankSum=2.858;QD=12.66;ReadPosRankSum=0.752;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|11),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|11),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|10),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 908823 . G A 12829.44 . AC=23;AF=0.1917;AN=120;BaseQRankSum=-0.746;DP=2383;Dels=0.00;FS=3.440;HRun=0;HaplotypeScore=1.6658;InbreedingCoeff=-0.0220;MQ=59.01;MQ0=0;MQRankSum=0.792;QD=15.61;ReadPosRankSum=1.037;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|11),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|12),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|11),UPSTREAM(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,upstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 909053 . G A 230.8 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.716;DP=1523;Dels=0.00;FS=1.669;HRun=1;HaplotypeScore=1.0522;InbreedingCoeff=-0.0166;MQ=59.32;MQ0=0;MQRankSum=-0.371;QD=14.43;ReadPosRankSum=0.854;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|1),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|12),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|13),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|12);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 909073 . C T 14861.74 . AC=51;AF=0.4250;AN=120;BaseQRankSum=-2.886;DP=1297;Dels=0.00;FS=1.649;HRun=1;HaplotypeScore=0.9560;InbreedingCoeff=-0.2187;MQ=59.41;MQ0=0;MQRankSum=-0.581;QD=17.08;ReadPosRankSum=-1.066;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|1),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|12),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|13),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|12);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 909221 . T C 1120.5 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-0.121;DP=1389;Dels=0.00;FS=9.505;HRun=0;HaplotypeScore=0.7261;InbreedingCoeff=-0.0453;MQ=58.91;MQ0=0;MQRankSum=0.284;QD=11.55;ReadPosRankSum=3.442;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),SYNONYMOUS_CODING(LOW|SILENT|agT/agC|S446|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|agT/agC|S46|176|PLEKHN1|protein_coding|CODING|ENST00000491024|),SYNONYMOUS_CODING(LOW|SILENT|agT/agC|S481|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|agT/agC|S533|663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,synonymous_variant|agT/agC|S|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|||46/176|protein_coding|1,synonymous_variant|agT/agC|S|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|||533/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,synonymous_variant|agT/agC|S|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|||446/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|agT/agC|S|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|||481/611|protein_coding|1 -chr1 909222 . G A 252.5 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.084;DP=1392;Dels=0.00;FS=1.211;HRun=0;HaplotypeScore=0.7091;InbreedingCoeff=-0.0090;MQ=58.92;MQ0=0;MQRankSum=0.700;QD=9.71;ReadPosRankSum=0.715;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A447T|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A47T|176|PLEKHN1|protein_coding|CODING|ENST00000491024|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A482T|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A534T|663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,missense_variant|Gcc/Acc|A/T|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|benign(0.273)|tolerated(0.13)|47/176|protein_coding|1,missense_variant|Gcc/Acc|A/T|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|benign(0.273)|tolerated(0.47)|534/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,missense_variant|Gcc/Acc|A/T|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|benign(0.273)|tolerated(0.34)|447/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,missense_variant|Gcc/Acc|A/T|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|benign(0.273)|tolerated(0.48)|482/611|protein_coding|1 -chr1 909238 . G C 30583.03 . AC=81;AF=0.6750;AN=120;BaseQRankSum=0.003;DP=1510;Dels=0.00;FS=15.547;HRun=1;HaplotypeScore=2.2700;InbreedingCoeff=-0.0333;MQ=58.99;MQ0=0;MQRankSum=-0.442;QD=22.47;ReadPosRankSum=2.247;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGt/cCt|R452P|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGt/cCt|R487P|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGt/cCt|R52P|176|PLEKHN1|protein_coding|CODING|ENST00000491024|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGt/cCt|R539P|663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,missense_variant|cGt/cCt|R/P|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|benign(0)|tolerated(0.17)|52/176|protein_coding|1,missense_variant|cGt/cCt|R/P|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|benign(0)|tolerated(0.25)|539/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,missense_variant|cGt/cCt|R/P|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|benign(0)|tolerated(0.23)|452/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,missense_variant|cGt/cCt|R/P|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|benign(0)|tolerated(0.23)|487/611|protein_coding|1 -chr1 909242 . A G 1739.34 . AC=6;AF=0.0500;AN=120;BaseQRankSum=1.162;DP=1524;Dels=0.00;FS=7.264;HRun=2;HaplotypeScore=2.2302;InbreedingCoeff=-0.0532;MQ=58.97;MQ0=0;MQRankSum=0.469;QD=11.75;ReadPosRankSum=2.640;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G453|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G488|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G53|176|PLEKHN1|protein_coding|CODING|ENST00000491024|),SYNONYMOUS_CODING(LOW|SILENT|ggA/ggG|G540|663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,synonymous_variant|ggA/ggG|G|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|||53/176|protein_coding|1,synonymous_variant|ggA/ggG|G|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|||540/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,synonymous_variant|ggA/ggG|G|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|||453/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|ggA/ggG|G|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|||488/611|protein_coding|1 -chr1 909309 . T C 7707.57 . AC=26;AF=0.2167;AN=120;BaseQRankSum=5.567;DP=1519;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.7523;InbreedingCoeff=-0.0786;MQ=58.79;MQ0=0;MQRankSum=1.234;QD=13.69;ReadPosRankSum=1.249;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tcc/Ccc|S476P|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tcc/Ccc|S511P|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tcc/Ccc|S563P|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tcc/Ccc|S76P|176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,missense_variant|Tcc/Ccc|S/P|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|benign(0.013)|tolerated(0.25)|76/176|protein_coding|1,missense_variant|Tcc/Ccc|S/P|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|benign(0.013)|tolerated(0.3)|563/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,missense_variant|Tcc/Ccc|S/P|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|benign(0)|tolerated(0.3)|476/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,missense_variant|Tcc/Ccc|S/P|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|benign(0.02)|tolerated(0.29)|511/611|protein_coding|1 -chr1 909320 . C T 232.62 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.426;DP=1387;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.8928;InbreedingCoeff=-0.0090;MQ=58.83;MQ0=0;MQRankSum=0.287;QD=10.57;ReadPosRankSum=2.476;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P479|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P514|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P566|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P79|176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,synonymous_variant|ccC/ccT|P|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|||79/176|protein_coding|1,synonymous_variant|ccC/ccT|P|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|||566/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,synonymous_variant|ccC/ccT|P|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|||479/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|ccC/ccT|P|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|||514/611|protein_coding|1 -chr1 909326 . C T 373.87 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.393;DP=1276;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.8069;InbreedingCoeff=-0.0180;MQ=58.80;MQ0=0;MQRankSum=0.099;QD=8.90;ReadPosRankSum=1.847;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G481|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G516|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G568|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G81|176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,synonymous_variant|ggC/ggT|G|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|||81/176|protein_coding|1,synonymous_variant|ggC/ggT|G|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|||568/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,synonymous_variant|ggC/ggT|G|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|||481/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|ggC/ggT|G|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|||516/611|protein_coding|1 -chr1 909363 . A C 203.29 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.679;DP=1010;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.5429;InbreedingCoeff=-0.0117;MQ=59.04;MQ0=0;MQRankSum=0.409;QD=11.29;ReadPosRankSum=2.880;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),SYNONYMOUS_CODING(LOW|SILENT|Aga/Cga|R494|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|Aga/Cga|R529|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|Aga/Cga|R581|663|PLEKHN1|protein_coding|CODING|ENST00000379409|),SYNONYMOUS_CODING(LOW|SILENT|Aga/Cga|R94|176|PLEKHN1|protein_coding|CODING|ENST00000491024|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,synonymous_variant|Aga/Cga|R|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|||94/176|protein_coding|1,synonymous_variant|Aga/Cga|R|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|||581/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,synonymous_variant|Aga/Cga|R|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|||494/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|Aga/Cga|R|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|||529/611|protein_coding|1 -chr1 909419 . C T 1837.1 . AC=16;AF=0.1333;AN=120;BaseQRankSum=-3.072;DP=694;Dels=0.00;FS=2.424;HRun=0;HaplotypeScore=0.2284;InbreedingCoeff=-0.0370;MQ=58.46;MQ0=0;MQRankSum=0.397;QD=12.17;ReadPosRankSum=1.761;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D112|176|PLEKHN1|protein_coding|CODING|ENST00000491024|),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D512|576|PLEKHN1|protein_coding|CODING|ENST00000379407|),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D547|611|PLEKHN1|protein_coding|CODING|ENST00000379410|),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D599|663|PLEKHN1|protein_coding|CODING|ENST00000379409|);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,synonymous_variant|gaC/gaT|D|ENSG00000187583|PLEKHN1|ENST00000491024|2/5|||112/176|protein_coding|1,synonymous_variant|gaC/gaT|D|ENSG00000187583|PLEKHN1|ENST00000379409|13/15|||599/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,synonymous_variant|gaC/gaT|D|ENSG00000187583|PLEKHN1|ENST00000379407|13/15|||512/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,synonymous_variant|gaC/gaT|D|ENSG00000187583|PLEKHN1|ENST00000379410|14/16|||547/611|protein_coding|1 -chr1 909523 . G A 539.82 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.755;DP=1030;Dels=0.00;FS=12.426;HRun=1;HaplotypeScore=0.5627;InbreedingCoeff=-0.0151;MQ=58.16;MQ0=0;MQRankSum=0.665;QD=10.80;ReadPosRankSum=0.177;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|2),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|13),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|14),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|13);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 909554 . CA C 2082.11 . AC=48;AF=0.4000;AN=120;BaseQRankSum=7.755;DP=1646;FS=15.954;HRun=1;HaplotypeScore=179.7893;InbreedingCoeff=-0.1499;MQ=58.00;MQ0=0;MQRankSum=2.728;QD=1.74;ReadPosRankSum=-12.687;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|2),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|13),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|14),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|13);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 909555 . A G 55343.84 . AC=113;AF=0.9417;AN=120;BaseQRankSum=2.487;DP=1646;Dels=0.01;FS=6.244;HRun=6;HaplotypeScore=1.1341;InbreedingCoeff=-0.0625;MQ=58.00;MQ0=0;MQRankSum=0.381;QD=33.62;ReadPosRankSum=-0.430;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|2),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|13),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|14),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|13);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 909768 . A G 183199.18 . AC=120;AF=1.0000;AN=120;BaseQRankSum=0.728;DP=5372;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=2.6976;InbreedingCoeff=-0.0000;MQ=58.76;MQ0=0;MQRankSum=2.388;QD=34.10;ReadPosRankSum=1.847;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|3),INTRON(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|14),INTRON(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|15),INTRON(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|14);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000379410||||-/611|protein_coding|1 -chr1 909960 . C T 318.47 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-0.416;DP=1592;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.2673;InbreedingCoeff=-0.0090;MQ=58.66;MQ0=0;MQRankSum=-0.101;QD=11.80;ReadPosRankSum=1.233;EFF=DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),UTR_3_PRIME(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|4),UTR_3_PRIME(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|15),UTR_3_PRIME(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|16),UTR_3_PRIME(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|15);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,3_prime_UTR_variant|||ENSG00000187583|PLEKHN1|ENST00000491024|4/5|||-/176|protein_coding|1,3_prime_UTR_variant|||ENSG00000187583|PLEKHN1|ENST00000379409|15/15|||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187583|PLEKHN1|ENST00000379407|15/15|||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187583|PLEKHN1|ENST00000379410|16/16|||-/611|protein_coding|1 -chr1 910394 . C T 398.09 . AC=13;AF=0.361;AN=36;BaseQRankSum=1.775;DP=46;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0554;InbreedingCoeff=0.3755;MQ=58.99;MQ0=0;MQRankSum=0.823;QD=23.42;ReadPosRankSum=0.051;EFF=DOWNSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|4),UTR_3_PRIME(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|15),UTR_3_PRIME(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|16);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187583|PLEKHN1|ENST00000379410|16/16|||-/611|protein_coding|1 -chr1 910438 . A G 1374.53 . AC=42;AF=1.000;AN=42;DP=42;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=-0.1458;MQ=57.77;MQ0=0;QD=33.53;EFF=DOWNSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|4),UTR_3_PRIME(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|15),UTR_3_PRIME(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|16);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187583|PLEKHN1|ENST00000379410|16/16|||-/611|protein_coding|1 -chr1 910473 . G A 299.74 . AC=11;AF=0.250;AN=44;BaseQRankSum=-1.316;DP=64;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0000;InbreedingCoeff=0.1771;MQ=59.28;MQ0=0;MQRankSum=0.212;QD=12.49;ReadPosRankSum=-0.144;EFF=DOWNSTREAM(MODIFIER||||663|PLEKHN1|protein_coding|CODING|ENST00000379409|),DOWNSTREAM(MODIFIER||||676|C1orf170|protein_coding|CODING|ENST00000341290|),DOWNSTREAM(MODIFIER||||696|C1orf170|protein_coding|CODING|ENST00000433179|),DOWNSTREAM(MODIFIER|||||C1orf170|retained_intron|CODING|ENST00000479361|),DOWNSTREAM(MODIFIER|||||PLEKHN1|retained_intron|CODING|ENST00000480267|),INTRON(MODIFIER||||176|PLEKHN1|protein_coding|CODING|ENST00000491024|4),UTR_3_PRIME(MODIFIER||||576|PLEKHN1|protein_coding|CODING|ENST00000379407|15),UTR_3_PRIME(MODIFIER||||611|PLEKHN1|protein_coding|CODING|ENST00000379410|16);CSQ=downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000341290||||-/676|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000433179||||-/696|protein_coding|1,intron_variant|||ENSG00000187583|PLEKHN1|ENST00000491024||||-/176|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379409||||-/663|protein_coding|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000480267|||||retained_intron|1,downstream_gene_variant|||ENSG00000187583|PLEKHN1|ENST00000379407||||-/576|protein_coding|1,downstream_gene_variant|||ENSG00000187642|C1orf170|ENST00000479361|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000187583|PLEKHN1|ENST00000379410|16/16|||-/611|protein_coding|1 -chr1 934099 . G C 525.65 . AC=24;AF=1.000;AN=24;DP=17;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=-0.1752;MQ=57.23;MQ0=0;QD=30.92;EFF=DOWNSTREAM(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|),DOWNSTREAM(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|),DOWNSTREAM(MODIFIER||||247|HES4|protein_coding|CODING|ENST00000428771|),DOWNSTREAM(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|),INTERGENIC(MODIFIER|||||||||);CSQ=downstream_gene_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000428771||||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000481869|||||retained_intron|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 -chr1 934106 . A G 492.03 . AC=22;AF=1.000;AN=22;DP=17;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=-0.1738;MQ=57.23;MQ0=0;QD=30.75;EFF=DOWNSTREAM(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|),DOWNSTREAM(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|),DOWNSTREAM(MODIFIER||||247|HES4|protein_coding|CODING|ENST00000428771|),DOWNSTREAM(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|),INTERGENIC(MODIFIER|||||||||);CSQ=downstream_gene_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000428771||||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000481869|||||retained_intron|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 -chr1 934130 . G C 64.94 . AC=4;AF=0.222;AN=18;BaseQRankSum=-0.306;DP=15;Dels=0.00;FS=2.515;HRun=0;HaplotypeScore=0.2219;MQ=56.85;MQ0=0;MQRankSum=-0.551;QD=12.99;ReadPosRankSum=2.388;EFF=DOWNSTREAM(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|),DOWNSTREAM(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|),DOWNSTREAM(MODIFIER||||247|HES4|protein_coding|CODING|ENST00000428771|),DOWNSTREAM(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|),INTERGENIC(MODIFIER|||||||||);CSQ=downstream_gene_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000428771||||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000481869|||||retained_intron|1,downstream_gene_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 -chr1 934797 . T G 52.82 . AC=6;AF=0.067;AN=90;BaseQRankSum=-1.481;DP=224;Dels=0.00;FS=43.747;HRun=4;HaplotypeScore=0.0853;InbreedingCoeff=-0.0492;MQ=49.44;MQ0=0;MQRankSum=-1.914;QD=1.51;ReadPosRankSum=0.524;EFF=EXON(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|2),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAc/gCc|D103A|221|HES4|protein_coding|CODING|ENST00000304952|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAc/gCc|D129A|247|HES4|protein_coding|CODING|ENST00000428771|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAc/gCc|D71A|189|HES4|protein_coding|CODING|ENST00000484667|);CSQ=missense_variant|gAc/gCc|D/A|ENSG00000188290|HES4|ENST00000484667|3/3|probably_damaging(0.994)|deleterious(0)|71/189|protein_coding|1,missense_variant|gAc/gCc|D/A|ENSG00000188290|HES4|ENST00000428771|3/3|benign(0.358)|deleterious(0)|129/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188290|HES4|ENST00000481869|2/2||||retained_intron|1,missense_variant|gAc/gCc|D/A|ENSG00000188290|HES4|ENST00000304952|4/4|probably_damaging(0.979)|deleterious(0)|103/221|protein_coding|1 -chr1 934888 . C A 233.29 . AC=2;AF=0.0172;AN=116;BaseQRankSum=-1.550;DP=738;Dels=0.00;FS=2.017;HRun=0;HaplotypeScore=1.1956;InbreedingCoeff=-0.0253;MQ=56.42;MQ0=0;MQRankSum=-0.145;QD=7.07;ReadPosRankSum=-0.918;EFF=INTRON(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|2),INTRON(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|3),INTRON(MODIFIER||||247|HES4|protein_coding|CODING|ENST00000428771|2),INTRON(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|1);CSQ=intron_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,intron_variant|||ENSG00000188290|HES4|ENST00000428771||||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,intron_variant&nc_transcript_variant|||ENSG00000188290|HES4|ENST00000481869|||||retained_intron|1,intron_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 -chr1 934990 . A G 108.0 . AC=12;AF=0.1017;AN=118;BaseQRankSum=-13.261;DP=1923;Dels=0.00;FS=97.152;HRun=3;HaplotypeScore=3.2884;InbreedingCoeff=-0.1051;MQ=46.21;MQ0=0;MQRankSum=-9.305;QD=0.35;ReadPosRankSum=0.938;EFF=EXON(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tcc/Ccc|S38P|189|HES4|protein_coding|CODING|ENST00000484667|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tcc/Ccc|S70P|221|HES4|protein_coding|CODING|ENST00000304952|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tcc/Ccc|S96P|247|HES4|protein_coding|CODING|ENST00000428771|);CSQ=missense_variant|Tcc/Ccc|S/P|ENSG00000188290|HES4|ENST00000484667|2/3|probably_damaging(0.979)|deleterious(0)|38/189|protein_coding|1,missense_variant|Tcc/Ccc|S/P|ENSG00000188290|HES4|ENST00000428771|2/3|benign(0.444)|deleterious(0.02)|96/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188290|HES4|ENST00000481869|1/2||||retained_intron|1,missense_variant|Tcc/Ccc|S/P|ENSG00000188290|HES4|ENST00000304952|3/4|possibly_damaging(0.534)|deleterious(0.01)|70/221|protein_coding|1 -chr1 935046 . G A 2684.85 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-3.713;DP=2876;Dels=0.00;FS=0.372;HRun=0;HaplotypeScore=3.4737;InbreedingCoeff=-0.0181;MQ=51.17;MQ0=0;MQRankSum=0.144;QD=11.88;ReadPosRankSum=0.789;EFF=EXON(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|1),INTRON(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|1),INTRON(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|2),INTRON(MODIFIER||||247|HES4|protein_coding|CODING|ENST00000428771|1);CSQ=intron_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,intron_variant|||ENSG00000188290|HES4|ENST00000428771||||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188290|HES4|ENST00000481869|1/2||||retained_intron|1,intron_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 -chr1 935222 . C A 15396.75 . AC=74;AF=0.6167;AN=120;BaseQRankSum=-9.471;DP=941;Dels=0.00;FS=3.802;HRun=0;HaplotypeScore=1.0918;InbreedingCoeff=-0.0313;MQ=53.20;MQ0=0;MQRankSum=2.504;QD=20.02;ReadPosRankSum=1.296;EFF=EXON(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|1),INTRON(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|1),INTRON(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|agG/agT|R44S|247|HES4|protein_coding|CODING|ENST00000428771|);CSQ=intron_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,missense_variant|agG/agT|R/S|ENSG00000188290|HES4|ENST00000428771|1/3|benign(0)|tolerated(0.95)|44/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188290|HES4|ENST00000481869|1/2||||retained_intron|1,intron_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 -chr1 935459 . A G 44.02 . AC=4;AF=0.67;AN=6;BaseQRankSum=-0.736;DP=4;Dels=0.00;FS=4.771;HRun=2;HaplotypeScore=0.0000;MQ=47.12;MQ0=0;MQRankSum=0.736;QD=22.01;ReadPosRankSum=0.736;EFF=EXON(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|1),UPSTREAM(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|),UTR_5_PRIME(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|1),UTR_5_PRIME(MODIFIER||||247|HES4|protein_coding|CODING|ENST00000428771|1);CSQ=upstream_gene_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,5_prime_UTR_variant|||ENSG00000188290|HES4|ENST00000428771|1/3|||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188290|HES4|ENST00000481869|1/2||||retained_intron|1,5_prime_UTR_variant|||ENSG00000188290|HES4|ENST00000304952|1/4|||-/221|protein_coding|1 -chr1 935492 . G T 38.54 . AC=4;AF=0.50;AN=8;BaseQRankSum=1.026;DP=5;Dels=0.00;FS=0.000;HRun=3;HaplotypeScore=0.2167;MQ=49.96;MQ0=0;MQRankSum=1.026;QD=19.27;ReadPosRankSum=-1.026;EFF=START_GAINED(LOW||||247|HES4|protein_coding|CODING|ENST00000428771|1),UPSTREAM(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|),UPSTREAM(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|),UPSTREAM(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|);CSQ=upstream_gene_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,5_prime_UTR_variant|||ENSG00000188290|HES4|ENST00000428771|1/3|||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,upstream_gene_variant|||ENSG00000188290|HES4|ENST00000481869|||||retained_intron|1,upstream_gene_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 -chr1 935715 . C T 119.53 . AC=6;AF=0.75;AN=8;BaseQRankSum=0.731;DP=6;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;MQ=60.00;MQ0=0;MQRankSum=0.731;QD=29.88;ReadPosRankSum=0.731;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|),UPSTREAM(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|),UPSTREAM(MODIFIER||||247|HES4|protein_coding|CODING|ENST00000428771|),UPSTREAM(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|);CSQ=upstream_gene_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,upstream_gene_variant|||ENSG00000188290|HES4|ENST00000428771||||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,upstream_gene_variant|||ENSG00000188290|HES4|ENST00000481869|||||retained_intron|1,upstream_gene_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 -chr1 935833 . C G 205.67 . AC=10;AF=1.00;AN=10;DP=7;Dels=0.00;FS=0.000;HRun=4;HaplotypeScore=0.0000;MQ=56.62;MQ0=0;QD=29.38;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||189|HES4|protein_coding|CODING|ENST00000484667|),UPSTREAM(MODIFIER||||221|HES4|protein_coding|CODING|ENST00000304952|),UPSTREAM(MODIFIER||||247|HES4|protein_coding|CODING|ENST00000428771|),UPSTREAM(MODIFIER|||||HES4|retained_intron|CODING|ENST00000481869|);CSQ=upstream_gene_variant|||ENSG00000188290|HES4|ENST00000484667||||-/189|protein_coding|1,upstream_gene_variant|||ENSG00000188290|HES4|ENST00000428771||||-/247|protein_coding|1,upstream_gene_variant|||ENSG00000272512|RP11-54O7.17|ENST00000606034|||||lincRNA|1,upstream_gene_variant|||ENSG00000188290|HES4|ENST00000481869|||||retained_intron|1,upstream_gene_variant|||ENSG00000188290|HES4|ENST00000304952||||-/221|protein_coding|1 -chr1 948421 . A AAAC 726.92 . AC=22;AF=1.000;AN=22;DP=20;FS=0.000;HRun=0;HaplotypeScore=4.9337;InbreedingCoeff=-0.1734;MQ=46.54;MQ0=0;QD=45.43;EFF=EXON(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|1),UPSTREAM(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|),UPSTREAM(MODIFIER|||||RP11-54O7.10|processed_pseudogene|NON_CODING|ENST00000423619|);CSQ=upstream_gene_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000231702|RP11-54O7.10|ENST00000423619|||||processed_pseudogene|1,non_coding_exon_variant&nc_transcript_variant&feature_elongation|||ENSG00000224969|RP11-54O7.11|ENST00000458555|1/2||||antisense|1 -chr1 948692 . G A 6273.5 . AC=98;AF=0.8909;AN=110;BaseQRankSum=0.890;DP=195;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1286;InbreedingCoeff=0.1904;MQ=57.72;MQ0=0;MQRankSum=0.244;QD=33.91;ReadPosRankSum=-0.202;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=upstream_gene_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 948844 . C CT 12812.2 . AC=110;AF=0.9167;AN=120;BaseQRankSum=12.848;DP=1613;FS=0.638;HRun=2;HaplotypeScore=267.6122;InbreedingCoeff=0.3293;MQ=57.07;MQ0=0;MQRankSum=-0.300;QD=8.17;ReadPosRankSum=0.729;EFF=UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|),UTR_5_PRIME(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1);CSQ=5_prime_UTR_variant&feature_elongation|||ENSG00000187608|ISG15|ENST00000379389|1/2|||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 948846 . T A 156.11 . AC=8;AF=0.0667;AN=120;BaseQRankSum=-3.754;DP=1643;Dels=0.00;FS=73.746;HRun=1;HaplotypeScore=23.6593;InbreedingCoeff=-0.0805;MQ=57.11;MQ0=0;MQRankSum=1.518;QD=0.75;ReadPosRankSum=-12.345;EFF=UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|),UTR_5_PRIME(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1);CSQ=5_prime_UTR_variant|||ENSG00000187608|ISG15|ENST00000379389|1/2|||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 948846 . T TA 63344.05 . AC=112;AF=0.9333;AN=120;BaseQRankSum=7.480;DP=1716;FS=0.000;HRun=1;HaplotypeScore=206.0209;InbreedingCoeff=-0.0721;MQ=56.50;MQ0=0;MQRankSum=0.146;QD=36.91;ReadPosRankSum=-0.823;EFF=UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|),UTR_5_PRIME(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1);CSQ=5_prime_UTR_variant&feature_elongation|||ENSG00000187608|ISG15|ENST00000379389|1/2|||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 948869 . C T 230.26 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.821;DP=2138;Dels=0.00;FS=1.991;HRun=0;HaplotypeScore=1.4232;InbreedingCoeff=-0.0088;MQ=56.52;MQ0=0;MQRankSum=2.379;QD=11.51;ReadPosRankSum=-0.420;EFF=UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|),UTR_5_PRIME(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1);CSQ=5_prime_UTR_variant|||ENSG00000187608|ISG15|ENST00000379389|1/2|||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 948870 . C G 69163.2 . AC=112;AF=0.9333;AN=120;BaseQRankSum=0.502;DP=2189;Dels=0.00;FS=1.037;HRun=1;HaplotypeScore=1.4157;InbreedingCoeff=-0.0719;MQ=56.60;MQ0=0;MQRankSum=-0.899;QD=31.60;ReadPosRankSum=-1.945;EFF=UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|),UTR_5_PRIME(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1);CSQ=5_prime_UTR_variant|||ENSG00000187608|ISG15|ENST00000379389|1/2|||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 948921 . T C 105377.26 . AC=112;AF=0.9333;AN=120;BaseQRankSum=0.554;DP=3568;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=2.5426;InbreedingCoeff=-0.0712;MQ=57.76;MQ0=0;MQRankSum=-1.329;QD=29.53;ReadPosRankSum=-3.423;EFF=UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|),UTR_5_PRIME(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1);CSQ=5_prime_UTR_variant|||ENSG00000187608|ISG15|ENST00000379389|1/2|||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 949053 . C G 1016.17 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.017;DP=2444;Dels=0.00;FS=9.350;HRun=2;HaplotypeScore=1.7725;InbreedingCoeff=0.6430;MQ=57.83;MQ0=0;MQRankSum=-0.809;QD=17.22;ReadPosRankSum=0.511;EFF=INTRON(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=intron_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 949123 . G A 102.54 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.767;DP=1126;Dels=0.00;FS=2.226;HRun=0;HaplotypeScore=0.7464;InbreedingCoeff=-0.0173;MQ=58.11;MQ0=0;MQRankSum=0.187;QD=6.03;ReadPosRankSum=-1.626;EFF=INTRON(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=intron_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 949235 . G A 32604.9 . AC=112;AF=0.9333;AN=120;BaseQRankSum=-0.988;DP=993;Dels=0.00;FS=7.691;HRun=0;HaplotypeScore=0.4657;InbreedingCoeff=-0.0787;MQ=58.29;MQ0=0;MQRankSum=-1.787;QD=32.83;ReadPosRankSum=-1.094;EFF=INTRON(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=intron_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 949302 . G A 113.2 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.449;DP=1914;Dels=0.00;FS=2.069;HRun=0;HaplotypeScore=1.3393;InbreedingCoeff=-0.0087;MQ=58.25;MQ0=0;MQRankSum=1.862;QD=8.71;ReadPosRankSum=1.655;EFF=INTRON(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|1),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=intron_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 949422 . G A 479.41 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.293;DP=3045;Dels=0.00;FS=6.680;HRun=1;HaplotypeScore=1.7335;InbreedingCoeff=-0.0084;MQ=58.77;MQ0=0;MQRankSum=-1.068;QD=12.29;ReadPosRankSum=-1.019;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGc/aAc|S21N|165|ISG15|protein_coding|CODING|ENST00000379389|),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=missense_variant|aGc/aAc|S/N|ENSG00000187608|ISG15|ENST00000379389|2/2|benign(0)|tolerated(0.74)|21/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 949608 . G A 34851.76 . AC=42;AF=0.3500;AN=120;BaseQRankSum=0.005;DP=2776;Dels=0.00;FS=11.466;HRun=1;HaplotypeScore=1.6893;InbreedingCoeff=-0.0991;MQ=58.46;MQ0=0;MQRankSum=1.157;QD=19.90;ReadPosRankSum=1.625;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGc/aAc|S83N|165|ISG15|protein_coding|CODING|ENST00000379389|),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=missense_variant|aGc/aAc|S/N|ENSG00000187608|ISG15|ENST00000379389|2/2|benign(0.002)|tolerated(0.25)|83/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 949654 . A G 103598.95 . AC=108;AF=0.9000;AN=120;BaseQRankSum=3.046;DP=3818;Dels=0.00;FS=9.083;HRun=0;HaplotypeScore=2.1911;InbreedingCoeff=0.0821;MQ=58.12;MQ0=0;MQRankSum=-0.993;QD=27.83;ReadPosRankSum=-2.811;EFF=SYNONYMOUS_CODING(LOW|SILENT|gtA/gtG|V98|165|ISG15|protein_coding|CODING|ENST00000379389|),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=synonymous_variant|gtA/gtG|V|ENSG00000187608|ISG15|ENST00000379389|2/2|||98/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 949655 . C T 133.5 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-2.364;DP=3843;Dels=0.00;FS=4.847;HRun=0;HaplotypeScore=2.2366;InbreedingCoeff=-0.0167;MQ=58.08;MQ0=0;MQRankSum=1.217;QD=2.97;ReadPosRankSum=-1.553;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgg/Tgg|R99W|165|ISG15|protein_coding|CODING|ENST00000379389|),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=missense_variant|Cgg/Tgg|R/W|ENSG00000187608|ISG15|ENST00000379389|2/2|benign(0.007)|tolerated(0.2)|99/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 949831 . C T 3120.79 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.506;DP=2204;Dels=0.00;FS=25.676;HRun=0;HaplotypeScore=2.0744;InbreedingCoeff=-0.0327;MQ=57.33;MQ0=0;MQRankSum=-0.396;QD=12.43;ReadPosRankSum=2.936;EFF=SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G157|165|ISG15|protein_coding|CODING|ENST00000379389|),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=synonymous_variant|ggC/ggT|G|ENSG00000187608|ISG15|ENST00000379389|2/2|||157/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 949832 . G A 1258.11 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.608;DP=2177;Dels=0.00;FS=0.646;HRun=0;HaplotypeScore=2.0191;InbreedingCoeff=-0.0169;MQ=57.31;MQ0=0;MQRankSum=2.168;QD=12.21;ReadPosRankSum=-0.092;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Agc|G158S|165|ISG15|protein_coding|CODING|ENST00000379389|),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=missense_variant|Ggc/Agc|G/S|ENSG00000187608|ISG15|ENST00000379389|2/2|benign(0.009)|tolerated(0.54)|158/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 949884 . A C 542.9 . AC=1;AF=0.0086;AN=116;BaseQRankSum=0.716;DP=1463;Dels=0.00;FS=4.086;HRun=0;HaplotypeScore=1.0630;InbreedingCoeff=-0.0204;MQ=58.08;MQ0=0;MQRankSum=-0.256;QD=14.67;ReadPosRankSum=3.251;EFF=UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|),UTR_3_PRIME(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|2);CSQ=3_prime_UTR_variant|||ENSG00000187608|ISG15|ENST00000379389|2/2|||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 949889 . G A 1254.48 . AC=2;AF=0.0172;AN=116;BaseQRankSum=-1.111;DP=1383;Dels=0.00;FS=3.846;HRun=2;HaplotypeScore=1.0736;InbreedingCoeff=-0.0309;MQ=58.18;MQ0=0;MQRankSum=-0.749;QD=12.93;ReadPosRankSum=2.198;EFF=UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|),UTR_3_PRIME(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|2);CSQ=3_prime_UTR_variant|||ENSG00000187608|ISG15|ENST00000379389|2/2|||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 949925 . C T 25659.52 . AC=103;AF=0.8879;AN=116;BaseQRankSum=0.388;DP=769;Dels=0.00;FS=1.403;HRun=0;HaplotypeScore=0.4309;InbreedingCoeff=0.1833;MQ=58.18;MQ0=0;MQRankSum=0.782;QD=33.54;ReadPosRankSum=-1.626;EFF=DOWNSTREAM(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=downstream_gene_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 950113 . GAAGT G 1437.6 . AC=33;AF=0.532;AN=62;BaseQRankSum=4.162;DP=62;FS=0.000;HRun=0;HaplotypeScore=9.8036;InbreedingCoeff=0.1341;MQ=49.27;MQ0=0;MQRankSum=-5.026;QD=34.23;ReadPosRankSum=-1.205;EFF=DOWNSTREAM(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=downstream_gene_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 950243 . A C 341.55 . AC=21;AF=0.553;AN=38;BaseQRankSum=1.286;DP=25;Dels=0.00;FS=2.282;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=0.2744;MQ=57.17;MQ0=0;MQRankSum=1.615;QD=24.40;ReadPosRankSum=0.099;EFF=DOWNSTREAM(MODIFIER||||165|ISG15|protein_coding|CODING|ENST00000379389|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||RP11-54O7.11|antisense|NON_CODING|ENST00000458555|);CSQ=downstream_gene_variant|||ENSG00000187608|ISG15|ENST00000379389||||-/165|protein_coding|1,upstream_gene_variant|||ENSG00000224969|RP11-54O7.11|ENST00000458555|||||antisense|1 -chr1 955016 . A G 41.68 . AC=4;AF=1.00;AN=4;DP=2;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.0000;MQ=47.12;MQ0=0;QD=20.84;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 -chr1 955213 . T C 205.28 . AC=12;AF=1.000;AN=12;DP=7;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.0000;MQ=49.17;MQ0=0;QD=29.33;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 -chr1 955597 . G T 397.16 . AC=19;AF=0.339;AN=56;BaseQRankSum=-2.859;DP=48;Dels=0.00;FS=4.867;HRun=0;HaplotypeScore=0.0297;InbreedingCoeff=0.3749;MQ=53.41;MQ0=0;MQRankSum=0.000;QD=22.06;ReadPosRankSum=2.226;EFF=SYNONYMOUS_CODING(LOW|SILENT|ccG/ccT|P15|2045|AGRN|protein_coding|CODING|ENST00000379370|);CSQ=synonymous_variant|ccG/ccT|P|ENSG00000188157|AGRN|ENST00000379370|1/36|||15/2045|protein_coding|1 -chr1 956061 . A AG 112.73 . AC=10;AF=0.556;AN=18;BaseQRankSum=0.851;DP=13;FS=0.000;HRun=7;HaplotypeScore=6.0534;MQ=48.24;MQ0=0;MQRankSum=-1.268;QD=22.55;ReadPosRankSum=0.103;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|1);CSQ=intron_variant&feature_elongation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 -chr1 956077 . C T 53.71 . AC=5;AF=0.250;AN=20;BaseQRankSum=1.109;DP=15;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=0.1223;MQ=51.77;MQ0=0;MQRankSum=0.370;QD=13.43;ReadPosRankSum=0.925;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 -chr1 957120 . G C 112.86 . AC=7;AF=0.250;AN=28;BaseQRankSum=-0.158;DP=18;Dels=0.00;FS=0.000;HRun=3;HaplotypeScore=0.0000;InbreedingCoeff=0.1430;MQ=57.39;MQ0=0;MQRankSum=-0.791;QD=18.81;ReadPosRankSum=0.791;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 -chr1 957474 . C G 88.48 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.393;DP=913;Dels=0.00;FS=13.654;HRun=0;HaplotypeScore=0.3942;InbreedingCoeff=-0.0138;MQ=58.75;MQ0=0;MQRankSum=0.079;QD=4.02;ReadPosRankSum=2.320;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 -chr1 957568 . A G 559.04 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.476;DP=2810;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.8451;InbreedingCoeff=-0.0085;MQ=58.63;MQ0=0;MQRankSum=0.035;QD=11.41;ReadPosRankSum=2.360;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 -chr1 957640 . C T 832.22 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.040;DP=3915;Dels=0.00;FS=3.301;HRun=0;HaplotypeScore=2.0922;InbreedingCoeff=-0.0143;MQ=58.81;MQ0=0;MQRankSum=0.961;QD=12.06;ReadPosRankSum=1.475;EFF=SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D87|2045|AGRN|protein_coding|CODING|ENST00000379370|);CSQ=synonymous_variant|gaC/gaT|D|ENSG00000188157|AGRN|ENST00000379370|2/36|||87/2045|protein_coding|1 -chr1 957667 . C T 1421.68 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-3.679;DP=4208;Dels=0.00;FS=3.179;HRun=3;HaplotypeScore=2.6513;InbreedingCoeff=-0.0085;MQ=58.87;MQ0=0;MQRankSum=-0.384;QD=13.94;ReadPosRankSum=1.504;EFF=SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G96|2045|AGRN|protein_coding|CODING|ENST00000379370|);CSQ=synonymous_variant|ggC/ggT|G|ENSG00000188157|AGRN|ENST00000379370|2/36|||96/2045|protein_coding|1 -chr1 957898 . G T 11511.79 . AC=35;AF=0.2917;AN=120;BaseQRankSum=-2.213;DP=1017;Dels=0.00;FS=12.996;HRun=0;HaplotypeScore=0.9917;InbreedingCoeff=-0.0796;MQ=55.78;MQ0=0;MQRankSum=2.025;QD=19.92;ReadPosRankSum=0.609;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 -chr1 957967 . T TTGTAGTCTGACCTGTGGTCTGAC 98446.19 . AC=111;AF=0.9737;AN=114;BaseQRankSum=-1.879;DP=448;FS=0.000;HRun=0;HaplotypeScore=117.9611;InbreedingCoeff=-0.0725;MQ=36.18;MQ0=0;MQRankSum=-4.523;QD=220.24;ReadPosRankSum=-0.243;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2);CSQ=intron_variant&feature_elongation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 -chr1 957971 . G A 6538.45 . AC=64;AF=0.5614;AN=114;BaseQRankSum=3.096;DP=423;Dels=0.00;FS=0.554;HRun=0;HaplotypeScore=1.4918;InbreedingCoeff=-0.6704;MQ=34.45;MQ0=0;MQRankSum=-1.012;QD=15.60;ReadPosRankSum=0.359;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 -chr1 957975 . C T 5244.2 . AC=61;AF=0.5446;AN=112;BaseQRankSum=3.205;DP=388;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.4483;InbreedingCoeff=-0.7134;MQ=33.55;MQ0=0;MQRankSum=-2.362;QD=13.66;ReadPosRankSum=-15.499;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 -chr1 957976 . A G 4910.47 . AC=60;AF=0.5455;AN=110;BaseQRankSum=4.309;DP=381;Dels=0.00;FS=1.187;HRun=0;HaplotypeScore=1.4201;InbreedingCoeff=-0.7088;MQ=32.86;MQ0=0;MQRankSum=-2.293;QD=13.03;ReadPosRankSum=-15.805;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 -chr1 958204 . C A 854.8 . AC=2;AF=0.025;AN=80;BaseQRankSum=1.546;DP=704;Dels=0.00;FS=3.962;HRun=1;HaplotypeScore=0.3471;InbreedingCoeff=-0.0900;MQ=57.09;MQ0=0;MQRankSum=0.211;QD=16.13;ReadPosRankSum=1.802;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 -chr1 958236 . T C 367.45 . AC=1;AF=0.013;AN=76;BaseQRankSum=-0.984;DP=1202;Dels=0.00;FS=6.974;HRun=2;HaplotypeScore=0.8541;InbreedingCoeff=-0.0759;MQ=56.81;MQ0=0;MQRankSum=0.871;QD=11.85;ReadPosRankSum=-0.134;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 -chr1 958248 . A G 14600.85 . AC=22;AF=0.297;AN=74;BaseQRankSum=6.262;DP=1397;Dels=0.00;FS=8.143;HRun=2;HaplotypeScore=1.8858;InbreedingCoeff=0.1339;MQ=57.01;MQ0=0;MQRankSum=0.270;QD=19.06;ReadPosRankSum=0.054;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1 -chr1 970215 . G C 1422.62 . AC=29;AF=0.483;AN=60;BaseQRankSum=-0.953;DP=94;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0644;InbreedingCoeff=0.4677;MQ=55.42;MQ0=0;MQRankSum=2.956;QD=30.27;ReadPosRankSum=1.616;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 970549 . TGG T 8449.83 . AC=65;AF=0.5603;AN=116;BaseQRankSum=-6.256;DP=1747;FS=120.761;HRun=13;HaplotypeScore=284.3804;InbreedingCoeff=-0.5216;MQ=44.59;MQ0=0;MQRankSum=0.141;QD=5.09;ReadPosRankSum=4.437;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|1);CSQ=intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000469403|1/3||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 970559 . GGGGT G 251.0 . AC=12;AF=0.1017;AN=118;BaseQRankSum=-0.439;DP=2064;FS=2.290;HRun=0;HaplotypeScore=346.6420;InbreedingCoeff=-0.1291;MQ=46.51;MQ0=0;MQRankSum=-12.374;QD=0.60;ReadPosRankSum=6.900;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|1);CSQ=intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000469403|1/3||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 970560 . GGGT G 6238.85 . AC=51;AF=0.4322;AN=118;BaseQRankSum=10.237;DP=2102;FS=59.155;HRun=0;HaplotypeScore=328.0111;InbreedingCoeff=-0.7958;MQ=46.78;MQ0=0;MQRankSum=-6.210;QD=3.10;ReadPosRankSum=17.494;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|1);CSQ=intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000469403|1/3||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 970561 . GGT G 13756.94 . AC=57;AF=0.4831;AN=118;BaseQRankSum=18.075;DP=2129;FS=78.149;HRun=0;HaplotypeScore=306.3538;InbreedingCoeff=-0.8937;MQ=47.00;MQ0=0;MQRankSum=-8.327;QD=6.50;ReadPosRankSum=23.377;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|1);CSQ=intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000469403|1/3||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 970562 . GT G 11726.17 . AC=56;AF=0.4746;AN=118;BaseQRankSum=20.100;DP=2148;FS=44.156;HRun=1;HaplotypeScore=301.2279;InbreedingCoeff=-0.8639;MQ=47.47;MQ0=0;MQRankSum=-8.868;QD=5.54;ReadPosRankSum=21.869;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|1);CSQ=intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000469403|1/3||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 970573 . T G 1645.49 . AC=31;AF=0.2583;AN=120;BaseQRankSum=-22.121;DP=2678;Dels=0.00;FS=172.614;HRun=4;HaplotypeScore=23.1118;InbreedingCoeff=-0.3662;MQ=49.95;MQ0=0;MQRankSum=-11.517;QD=1.06;ReadPosRankSum=19.788;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|2),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000469403|1/3||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 970765 . C T 1353.46 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.165;DP=4536;Dels=0.00;FS=22.500;HRun=0;HaplotypeScore=2.7925;InbreedingCoeff=-0.0084;MQ=57.72;MQ0=0;MQRankSum=1.115;QD=12.65;ReadPosRankSum=3.005;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|3),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|2),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 970836 . G A 214.73 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.962;DP=2105;Dels=0.00;FS=7.794;HRun=0;HaplotypeScore=1.1718;InbreedingCoeff=-0.0084;MQ=57.64;MQ0=0;MQRankSum=0.008;QD=10.74;ReadPosRankSum=1.304;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|3),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|2),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 970915 . C T 377.1 . AC=2;AF=0.0167;AN=120;BaseQRankSum=2.244;DP=644;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.3824;InbreedingCoeff=-0.0289;MQ=57.36;MQ0=0;MQRankSum=0.823;QD=9.20;ReadPosRankSum=1.968;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|3),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|2),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 971224 . A G 877.98 . AC=35;AF=0.921;AN=38;BaseQRankSum=0.367;DP=38;Dels=0.00;FS=6.105;HRun=6;HaplotypeScore=0.1297;InbreedingCoeff=0.0071;MQ=58.78;MQ0=0;MQRankSum=0.367;QD=27.44;ReadPosRankSum=0.768;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|3),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|2),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 975702 . A G 736.88 . AC=30;AF=0.833;AN=36;BaseQRankSum=1.380;DP=25;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.0438;InbreedingCoeff=0.2327;MQ=57.17;MQ0=0;MQRankSum=0.794;QD=33.49;ReadPosRankSum=-0.544;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|3),INTRON(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|2),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|1);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 976428 . C A 86.19 . AC=8;AF=1.00;AN=8;DP=10;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5990;MQ=29.00;MQ0=0;QD=21.55;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|4),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|2),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 976514 . C A 153.87 . AC=10;AF=0.833;AN=12;BaseQRankSum=0.742;DP=14;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0000;MQ=40.36;MQ0=0;MQRankSum=-0.742;QD=25.65;ReadPosRankSum=-0.742;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|4),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|2),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 977203 . G C 14246.35 . AC=102;AF=0.8947;AN=114;BaseQRankSum=0.075;DP=497;Dels=0.00;FS=12.091;HRun=1;HaplotypeScore=0.3092;InbreedingCoeff=0.1183;MQ=53.27;MQ0=0;MQRankSum=-0.225;QD=29.99;ReadPosRankSum=0.784;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|6),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 977220 . C T 77.62 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.664;DP=734;Dels=0.00;FS=6.335;HRun=1;HaplotypeScore=0.5865;InbreedingCoeff=-0.0433;MQ=54.41;MQ0=0;MQRankSum=-0.052;QD=3.53;ReadPosRankSum=0.239;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|6),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 977294 . G A 323.25 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.012;DP=2064;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.9641;InbreedingCoeff=-0.0130;MQ=55.98;MQ0=0;MQRankSum=1.642;QD=9.51;ReadPosRankSum=0.377;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|6),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 977330 . T C 75130.94 . AC=107;AF=0.8917;AN=120;BaseQRankSum=4.114;DP=2805;Dels=0.00;FS=1.138;HRun=2;HaplotypeScore=2.7705;InbreedingCoeff=0.2224;MQ=56.35;MQ0=0;MQRankSum=1.859;QD=27.97;ReadPosRankSum=-0.230;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|6),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=splice_region_variant&intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 977356 . C T 37.67 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.314;DP=3180;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=2.0624;InbreedingCoeff=-0.0085;MQ=56.04;MQ0=0;MQRankSum=1.209;QD=3.42;ReadPosRankSum=0.828;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgg/Tgg|R400W|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=missense_variant|Cgg/Tgg|R/W|ENSG00000188157|AGRN|ENST00000379370|7/36|benign(0.061)|deleterious(0.02)|400/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 977570 . G A 23442.1 . AC=102;AF=0.8500;AN=120;BaseQRankSum=-2.570;DP=882;Dels=0.00;FS=3.065;HRun=0;HaplotypeScore=1.0563;InbreedingCoeff=0.1840;MQ=56.97;MQ0=0;MQRankSum=1.250;QD=27.84;ReadPosRankSum=-0.551;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|7),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 977780 . C T 1896.26 . AC=55;AF=0.833;AN=66;BaseQRankSum=-0.091;DP=70;Dels=0.00;FS=1.840;HRun=0;HaplotypeScore=0.0570;InbreedingCoeff=0.2412;MQ=56.97;MQ0=0;MQRankSum=0.071;QD=30.10;ReadPosRankSum=0.112;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|7),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 978183 . C G 34.17 . AC=2;AF=0.043;AN=46;BaseQRankSum=1.339;DP=53;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0869;InbreedingCoeff=0.1027;MQ=58.24;MQ0=0;MQRankSum=1.491;QD=17.09;ReadPosRankSum=0.025;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|7),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 978193 . G A 202.48 . AC=6;AF=0.125;AN=48;BaseQRankSum=0.284;DP=51;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.0833;InbreedingCoeff=-0.1181;MQ=58.63;MQ0=0;MQRankSum=-0.041;QD=14.46;ReadPosRankSum=0.527;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|7),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 978576 . A AG 514.05 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.254;DP=2624;FS=0.000;HRun=7;HaplotypeScore=255.5270;InbreedingCoeff=-0.0227;MQ=57.44;MQ0=0;MQRankSum=-3.300;QD=9.70;ReadPosRankSum=-2.347;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|7),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 978600 . GCC G 17413.56 . AC=61;AF=0.5169;AN=118;BaseQRankSum=43.323;DP=3497;FS=2.177;HRun=4;HaplotypeScore=348.2579;InbreedingCoeff=-0.1331;MQ=57.63;MQ0=0;MQRankSum=3.380;QD=6.27;ReadPosRankSum=3.269;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|7),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 978603 . CCT C 71936.99 . AC=63;AF=0.5339;AN=118;BaseQRankSum=27.120;DP=3635;FS=0.415;HRun=0;HaplotypeScore=268.7446;InbreedingCoeff=-0.1355;MQ=57.70;MQ0=0;MQRankSum=-0.052;QD=24.97;ReadPosRankSum=2.237;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|7),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 978604 . CTG C 22255.35 . AC=63;AF=0.5339;AN=118;BaseQRankSum=42.643;DP=3702;FS=4.247;HRun=0;HaplotypeScore=358.4425;InbreedingCoeff=-0.1343;MQ=57.72;MQ0=0;MQRankSum=2.535;QD=7.58;ReadPosRankSum=3.410;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|7),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 978762 . G A 3088.0 . AC=4;AF=0.0333;AN=120;BaseQRankSum=0.970;DP=3629;Dels=0.00;FS=0.380;HRun=0;HaplotypeScore=2.3356;InbreedingCoeff=-0.0359;MQ=57.56;MQ0=0;MQRankSum=1.561;QD=14.64;ReadPosRankSum=0.622;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Agc|G510S|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|Ggc/Agc|G/S|ENSG00000188157|AGRN|ENST00000379370|8/36|probably_damaging(0.961)|tolerated(0.05)|510/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 978850 . T G 355.87 . AC=13;AF=0.1083;AN=120;BaseQRankSum=-20.130;DP=2142;Dels=0.00;FS=811.949;HRun=3;HaplotypeScore=4.6408;InbreedingCoeff=-0.1393;MQ=53.60;MQ0=0;MQRankSum=-8.269;QD=0.90;ReadPosRankSum=-1.238;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|8),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 978857 . T G 60.53 . AC=8;AF=0.0667;AN=120;BaseQRankSum=-18.263;DP=2025;Dels=0.01;FS=633.354;HRun=4;HaplotypeScore=4.9061;InbreedingCoeff=-0.0867;MQ=53.43;MQ0=0;MQRankSum=-5.927;QD=0.18;ReadPosRankSum=3.709;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|8),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 979594 . C T 694.16 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.081;DP=5875;DS;Dels=0.00;FS=21.795;HRun=0;HaplotypeScore=3.6311;InbreedingCoeff=-0.0090;MQ=57.97;MQ0=0;MQRankSum=1.076;QD=13.10;ReadPosRankSum=-2.987;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P702L|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|cCg/cTg|P/L|ENSG00000188157|AGRN|ENST00000379370|11/36|probably_damaging(0.931)|tolerated(0.29)|702/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 979669 . G A 1676.8 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-2.241;DP=3939;DS;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=4.9042;InbreedingCoeff=-0.0141;MQ=56.95;MQ0=0;MQRankSum=-0.084;QD=10.10;ReadPosRankSum=1.062;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|11),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 979690 . G A 3048.77 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-3.190;DP=3529;Dels=0.00;FS=4.170;HRun=0;HaplotypeScore=1.8409;InbreedingCoeff=-0.0220;MQ=57.08;MQ0=0;MQRankSum=0.399;QD=11.82;ReadPosRankSum=-0.422;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|11),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 979748 . A T 1423.3 . AC=2;AF=0.0167;AN=120;BaseQRankSum=0.288;DP=3261;Dels=0.00;FS=1.119;HRun=0;HaplotypeScore=1.7332;InbreedingCoeff=-0.0175;MQ=58.27;MQ0=0;MQRankSum=0.306;QD=11.21;ReadPosRankSum=1.431;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gAg/gTg|E728V|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|gAg/gTg|E/V|ENSG00000188157|AGRN|ENST00000379370|12/36|probably_damaging(0.932)|deleterious(0.02)|728/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 979835 . G A 1973.32 . AC=2;AF=0.0169;AN=118;BaseQRankSum=-1.744;DP=1701;Dels=0.00;FS=1.140;HRun=0;HaplotypeScore=1.2871;InbreedingCoeff=-0.0241;MQ=58.02;MQ0=0;MQRankSum=1.712;QD=14.00;ReadPosRankSum=0.225;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|12),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 979890 . C T 106.44 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.494;DP=863;Dels=0.00;FS=2.607;HRun=0;HaplotypeScore=0.6567;InbreedingCoeff=-0.0292;MQ=58.16;MQ0=0;MQRankSum=-0.586;QD=9.68;ReadPosRankSum=0.909;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|12),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 980077 . C T 58.05 . AC=1;AF=0.012;AN=86;BaseQRankSum=-0.500;DP=105;Dels=0.00;FS=12.895;HRun=0;HaplotypeScore=0.1629;InbreedingCoeff=-0.1341;MQ=58.00;MQ0=0;MQRankSum=0.378;QD=11.61;ReadPosRankSum=0.765;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|12),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 980189 . G A 62.31 . AC=2;AF=0.032;AN=62;BaseQRankSum=-0.689;DP=65;Dels=0.00;FS=2.622;HRun=0;HaplotypeScore=0.0923;InbreedingCoeff=0.0483;MQ=58.57;MQ0=0;MQRankSum=-0.262;QD=20.77;ReadPosRankSum=-0.131;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|12),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 980276 . C T 58.0 . AC=2;AF=0.021;AN=96;BaseQRankSum=-2.227;DP=125;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1208;InbreedingCoeff=0.0215;MQ=58.51;MQ0=0;MQRankSum=0.682;QD=14.50;ReadPosRankSum=-0.682;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|12),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 980460 . G A 26594.59 . AC=107;AF=0.8917;AN=120;BaseQRankSum=-2.656;DP=975;Dels=0.00;FS=0.883;HRun=0;HaplotypeScore=0.8473;InbreedingCoeff=0.2169;MQ=58.13;MQ0=0;MQRankSum=-0.492;QD=28.60;ReadPosRankSum=0.989;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|12),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 980824 . G C 275.83 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.440;DP=1969;Dels=0.00;FS=12.667;HRun=0;HaplotypeScore=2.5993;InbreedingCoeff=-0.0094;MQ=58.48;MQ0=0;MQRankSum=1.465;QD=11.99;ReadPosRankSum=-0.443;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|1),SYNONYMOUS_CODING(LOW|SILENT|ggG/ggC|G819|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|ggG/ggC|G|ENSG00000188157|AGRN|ENST00000379370|14/36|||819/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000479707|1/4||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 980941 . C T 600.94 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.751;DP=2190;Dels=0.00;FS=4.066;HRun=0;HaplotypeScore=1.5643;InbreedingCoeff=-0.0087;MQ=58.24;MQ0=0;MQRankSum=0.449;QD=12.02;ReadPosRankSum=0.589;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|14),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 980948 . C T 538.98 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.096;DP=2144;Dels=0.00;FS=17.669;HRun=0;HaplotypeScore=2.3599;InbreedingCoeff=-0.0087;MQ=58.24;MQ0=0;MQRankSum=-0.314;QD=17.39;ReadPosRankSum=0.302;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|14),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 980955 . AC A 1099.14 . AC=2;AF=0.0167;AN=120;BaseQRankSum=8.294;DP=2110;FS=28.545;HRun=5;HaplotypeScore=116.2371;InbreedingCoeff=-0.0170;MQ=58.34;MQ0=0;MQRankSum=-0.585;QD=9.16;ReadPosRankSum=1.829;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|14),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 981087 . A G 111674.9 . AC=105;AF=0.8750;AN=120;BaseQRankSum=2.112;DP=3879;Dels=0.00;FS=2.321;HRun=0;HaplotypeScore=2.2416;InbreedingCoeff=0.1619;MQ=58.40;MQ0=0;MQRankSum=-0.442;QD=29.96;ReadPosRankSum=-1.459;EFF=DOWNSTREAM(MODIFIER|||||AGRN|processed_transcript|CODING|ENST00000477585|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|14),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000477585|||||processed_transcript|1 -chr1 981131 . A G 3064.9 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-1.865;DP=5182;Dels=0.00;FS=11.038;HRun=0;HaplotypeScore=2.4921;InbreedingCoeff=-0.0256;MQ=58.34;MQ0=0;MQRankSum=1.546;QD=10.79;ReadPosRankSum=1.415;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000469403|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|2),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cAa/cGa|Q852R|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|cAa/cGa|Q/R|ENSG00000188157|AGRN|ENST00000379370|15/36|benign(0.227)|tolerated(0.44)|852/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000479707|2/4||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000469403|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 981931 . A G 38481.37 . AC=102;AF=0.8500;AN=120;BaseQRankSum=4.244;DP=1481;Dels=0.00;FS=5.461;HRun=0;HaplotypeScore=0.5832;InbreedingCoeff=0.0829;MQ=57.68;MQ0=0;MQRankSum=2.481;QD=26.85;ReadPosRankSum=-2.762;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),SYNONYMOUS_CODING(LOW|SILENT|tcA/tcG|S1022|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|tcA/tcG|S|ENSG00000188157|AGRN|ENST00000379370|18/36|||1022/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 982205 . G A 372.27 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.314;DP=1529;Dels=0.00;FS=1.129;HRun=1;HaplotypeScore=1.1177;InbreedingCoeff=-0.0134;MQ=58.00;MQ0=0;MQRankSum=0.488;QD=14.32;ReadPosRankSum=-0.923;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gag/Aag|E1086K|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|Gag/Aag|E/K|ENSG00000188157|AGRN|ENST00000379370|19/36|benign(0.32)|tolerated(0.41)|1086/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 982403 . C T 1164.33 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-2.215;DP=1442;Dels=0.00;FS=2.421;HRun=1;HaplotypeScore=0.9843;InbreedingCoeff=-0.0334;MQ=57.51;MQ0=0;MQRankSum=0.119;QD=10.49;ReadPosRankSum=-1.415;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|19),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 982444 . A G 24810.69 . AC=102;AF=0.8500;AN=120;BaseQRankSum=4.056;DP=908;Dels=0.00;FS=0.946;HRun=3;HaplotypeScore=0.5521;InbreedingCoeff=0.0418;MQ=57.33;MQ0=0;MQRankSum=0.383;QD=27.94;ReadPosRankSum=1.506;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|19),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 982462 . T C 27335.24 . AC=119;AF=0.9917;AN=120;BaseQRankSum=0.923;DP=796;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5400;InbreedingCoeff=-0.0264;MQ=57.04;MQ0=0;MQRankSum=0.157;QD=34.34;ReadPosRankSum=-1.750;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|19),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 982482 . C T 1093.25 . AC=3;AF=0.0254;AN=118;BaseQRankSum=0.161;DP=714;Dels=0.00;FS=1.368;HRun=0;HaplotypeScore=0.5694;InbreedingCoeff=-0.0414;MQ=57.13;MQ0=0;MQRankSum=0.077;QD=14.20;ReadPosRankSum=3.205;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|19),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 982484 . C T 1096.42 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.607;DP=707;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5118;InbreedingCoeff=-0.0484;MQ=57.24;MQ0=0;MQRankSum=0.555;QD=14.43;ReadPosRankSum=3.144;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|19),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 982513 . T C 22111.8 . AC=107;AF=0.8917;AN=120;BaseQRankSum=1.897;DP=701;Dels=0.00;FS=6.392;HRun=0;HaplotypeScore=0.5397;InbreedingCoeff=0.3020;MQ=57.59;MQ0=0;MQRankSum=-0.108;QD=32.37;ReadPosRankSum=0.738;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|19),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 982573 . C T 1076.33 . AC=3;AF=0.0250;AN=120;BaseQRankSum=0.536;DP=905;Dels=0.00;FS=13.480;HRun=0;HaplotypeScore=0.6754;InbreedingCoeff=-0.0368;MQ=58.37;MQ0=0;MQRankSum=-0.537;QD=12.23;ReadPosRankSum=1.369;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|19),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 982844 . G C 4135.57 . AC=11;AF=0.0917;AN=120;BaseQRankSum=-1.942;DP=1958;Dels=0.00;FS=2.777;HRun=0;HaplotypeScore=1.2617;InbreedingCoeff=-0.1015;MQ=58.19;MQ0=0;MQRankSum=1.346;QD=12.09;ReadPosRankSum=2.109;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|20),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 982865 . A G 36.62 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.110;DP=1923;Dels=0.00;FS=2.301;HRun=0;HaplotypeScore=1.0780;InbreedingCoeff=-0.0085;MQ=58.17;MQ0=0;MQRankSum=-1.323;QD=5.23;ReadPosRankSum=1.132;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|20),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000478677|1/2||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 982906 . G A 170.75 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.574;DP=1942;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.9590;InbreedingCoeff=-0.0085;MQ=58.80;MQ0=0;MQRankSum=0.287;QD=11.38;ReadPosRankSum=-1.149;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|20),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000478677|1/2||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 982941 . T C 66725.31 . AC=101;AF=0.8417;AN=120;BaseQRankSum=6.926;DP=2345;Dels=0.00;FS=18.520;HRun=3;HaplotypeScore=1.4495;InbreedingCoeff=0.1870;MQ=58.87;MQ0=0;MQRankSum=0.922;QD=29.91;ReadPosRankSum=-2.729;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|20),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000478677|1/2||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 982994 . T C 93842.13 . AC=104;AF=0.8667;AN=120;BaseQRankSum=1.394;DP=3268;Dels=0.00;FS=0.376;HRun=1;HaplotypeScore=1.8496;InbreedingCoeff=0.1346;MQ=58.74;MQ0=0;MQRankSum=1.734;QD=30.11;ReadPosRankSum=-2.989;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|2),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|1),SYNONYMOUS_CODING(LOW|SILENT|ttT/ttC|F1186|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000466223|2/3||||retained_intron|1,synonymous_variant|ttT/ttC|F|ENSG00000188157|AGRN|ENST00000379370|21/36|||1186/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000478677|1/2||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 983005 . G A 980.3 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.923;DP=3454;Dels=0.00;FS=1.269;HRun=0;HaplotypeScore=1.9623;InbreedingCoeff=-0.0084;MQ=58.65;MQ0=0;MQRankSum=1.298;QD=13.62;ReadPosRankSum=2.603;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|2),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGc/cAc|R1190H|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000466223|2/3||||retained_intron|1,missense_variant|cGc/cAc|R/H|ENSG00000188157|AGRN|ENST00000379370|21/36|benign(0.252)|tolerated(0.19)|1190/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000478677|1/2||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 983291 . C G 40.75 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-3.182;DP=508;Dels=0.00;FS=19.277;HRun=1;HaplotypeScore=0.5577;InbreedingCoeff=-0.0586;MQ=54.43;MQ0=0;MQRankSum=-1.088;QD=0.85;ReadPosRankSum=-2.005;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|22),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 984147 . CAAAAAAAAAAA C 4515.7 . AC=46;AF=0.460;AN=100;BaseQRankSum=7.017;DP=179;FS=0.000;HRun=22;HaplotypeScore=47.7401;InbreedingCoeff=0.1656;MQ=37.04;MQ0=0;MQRankSum=-0.993;QD=36.71;ReadPosRankSum=4.302;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|23),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000492947|1/2||||retained_intron|1 -chr1 984170 . GCA G 2088.76 . AC=73;AF=0.6404;AN=114;BaseQRankSum=8.221;DP=284;FS=0.000;HRun=0;HaplotypeScore=52.8663;InbreedingCoeff=0.0057;MQ=43.30;MQ0=0;MQRankSum=-4.981;QD=8.29;ReadPosRankSum=6.043;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|23),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000492947|1/2||||retained_intron|1 -chr1 984171 . CAG C 6456.64 . AC=72;AF=0.6207;AN=116;BaseQRankSum=5.599;DP=294;FS=0.921;HRun=0;HaplotypeScore=45.6091;InbreedingCoeff=-0.0184;MQ=43.76;MQ0=0;MQRankSum=-4.138;QD=26.03;ReadPosRankSum=5.090;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|23),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000492947|1/2||||retained_intron|1 -chr1 984202 . A G 219.31 . AC=2;AF=0.0169;AN=118;BaseQRankSum=-2.549;DP=472;Dels=0.00;FS=8.756;HRun=0;HaplotypeScore=1.0667;InbreedingCoeff=-0.0548;MQ=49.93;MQ0=0;MQRankSum=1.399;QD=7.83;ReadPosRankSum=0.241;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|23),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|1/2||||retained_intron|1 -chr1 984257 . C T 182.2 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.337;DP=770;Dels=0.00;FS=9.908;HRun=0;HaplotypeScore=0.4996;InbreedingCoeff=-0.0252;MQ=54.84;MQ0=0;MQRankSum=1.233;QD=12.15;ReadPosRankSum=-0.369;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A1372|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|gcC/gcT|A|ENSG00000188157|AGRN|ENST00000379370|24/36|||1372/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|1/2||||retained_intron|1 -chr1 984272 . C T 98.86 . AC=2;AF=0.0169;AN=118;BaseQRankSum=-0.517;DP=781;Dels=0.00;FS=1.477;HRun=2;HaplotypeScore=0.5249;InbreedingCoeff=-0.0341;MQ=54.72;MQ0=0;MQRankSum=0.805;QD=3.95;ReadPosRankSum=0.353;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F1377|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|ttC/ttT|F|ENSG00000188157|AGRN|ENST00000379370|24/36|||1377/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|1/2||||retained_intron|1 -chr1 984302 . T C 14311.5 . AC=73;AF=0.6186;AN=118;BaseQRankSum=1.074;DP=888;Dels=0.00;FS=38.864;HRun=1;HaplotypeScore=0.6522;InbreedingCoeff=-0.0933;MQ=54.70;MQ0=0;MQRankSum=1.221;QD=18.54;ReadPosRankSum=0.025;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),SYNONYMOUS_CODING(LOW|SILENT|acT/acC|T1387|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|acT/acC|T|ENSG00000188157|AGRN|ENST00000379370|24/36|||1387/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|1/2||||retained_intron|1 -chr1 984456 . C T 37.75 . AC=2;AF=0.0175;AN=114;BaseQRankSum=0.621;DP=348;Dels=0.01;FS=0.000;HRun=0;HaplotypeScore=0.7641;InbreedingCoeff=-0.0607;MQ=53.36;MQ0=0;MQRankSum=0.580;QD=2.90;ReadPosRankSum=-0.096;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 984476 . T G 35.43 . AC=6;AF=0.060;AN=100;BaseQRankSum=-3.204;DP=227;Dels=0.01;FS=3.373;HRun=3;HaplotypeScore=0.9086;InbreedingCoeff=-0.1488;MQ=48.96;MQ0=0;MQRankSum=-3.544;QD=1.07;ReadPosRankSum=1.813;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 984480 . C G 43.98 . AC=7;AF=0.071;AN=98;BaseQRankSum=-3.119;DP=215;Dels=0.00;FS=2.297;HRun=4;HaplotypeScore=2.0881;InbreedingCoeff=-0.1728;MQ=47.60;MQ0=0;MQRankSum=-4.127;QD=1.47;ReadPosRankSum=3.119;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 984485 . A G 61.07 . AC=8;AF=0.083;AN=96;BaseQRankSum=-3.712;DP=196;Dels=0.02;FS=2.293;HRun=4;HaplotypeScore=2.7170;InbreedingCoeff=-0.0981;MQ=45.76;MQ0=0;MQRankSum=-5.008;QD=2.35;ReadPosRankSum=3.158;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 984489 . G T 64.93 . AC=2;AF=0.021;AN=96;BaseQRankSum=1.248;DP=184;Dels=0.00;FS=2.430;HRun=0;HaplotypeScore=2.0202;InbreedingCoeff=0.0358;MQ=44.34;MQ0=0;MQRankSum=0.975;QD=21.64;ReadPosRankSum=-0.342;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 984489 . GAC G 147.88 . AC=19;AF=0.1863;AN=102;BaseQRankSum=5.670;DP=170;FS=16.798;HRun=0;HaplotypeScore=44.7087;InbreedingCoeff=-0.0321;MQ=43.36;MQ0=0;MQRankSum=-5.590;QD=2.39;ReadPosRankSum=-1.803;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 984494 . GC G 1466.1 . AC=57;AF=0.663;AN=86;BaseQRankSum=2.571;DP=152;FS=2.990;HRun=1;HaplotypeScore=47.8858;InbreedingCoeff=0.1367;MQ=40.49;MQ0=0;MQRankSum=-2.751;QD=11.64;ReadPosRankSum=0.066;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 984501 . A G 452.7 . AC=26;AF=0.317;AN=82;BaseQRankSum=0.750;DP=155;Dels=0.04;FS=57.535;HRun=7;HaplotypeScore=2.7737;InbreedingCoeff=-0.1078;MQ=40.74;MQ0=0;MQRankSum=-5.758;QD=5.73;ReadPosRankSum=3.176;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 984510 . C CG 61.68 . AC=13;AF=0.144;AN=90;BaseQRankSum=4.890;DP=164;FS=70.003;HRun=4;HaplotypeScore=50.6057;InbreedingCoeff=-0.0152;MQ=42.03;MQ0=0;MQRankSum=-4.938;QD=1.50;ReadPosRankSum=-1.662;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 984524 . T G 47.35 . AC=11;AF=0.125;AN=88;BaseQRankSum=-2.019;DP=191;Dels=0.01;FS=48.423;HRun=3;HaplotypeScore=3.1841;InbreedingCoeff=-0.0001;MQ=47.05;MQ0=0;MQRankSum=-3.798;QD=1.01;ReadPosRankSum=3.652;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 984528 . T G 133.69 . AC=15;AF=0.167;AN=90;BaseQRankSum=-2.316;DP=208;Dels=0.01;FS=63.386;HRun=4;HaplotypeScore=2.2156;InbreedingCoeff=-0.0134;MQ=47.68;MQ0=0;MQRankSum=-5.136;QD=2.09;ReadPosRankSum=3.074;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 984533 . T G 92.45 . AC=10;AF=0.111;AN=90;BaseQRankSum=-2.508;DP=221;Dels=0.00;FS=75.206;HRun=4;HaplotypeScore=0.9453;InbreedingCoeff=0.0314;MQ=49.13;MQ0=0;MQRankSum=-5.147;QD=1.71;ReadPosRankSum=0.969;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|24),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|1),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 984711 . G A 778.12 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.444;DP=2749;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.7477;InbreedingCoeff=-0.0101;MQ=58.27;MQ0=0;MQRankSum=-0.471;QD=11.61;ReadPosRankSum=-0.316;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|2),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGg/cAg|R1465Q|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|cGg/cAg|R/Q|ENSG00000188157|AGRN|ENST00000379370|25/36|benign(0.094)|tolerated(0.53)|1465/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000492947|2/2||||retained_intron|1 -chr1 984847 . G C 1888.19 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.596;DP=3896;Dels=0.00;FS=5.841;HRun=0;HaplotypeScore=3.8645;InbreedingCoeff=-0.0190;MQ=58.46;MQ0=0;MQRankSum=0.505;QD=13.58;ReadPosRankSum=-1.173;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|25),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 984888 . A G 1593.23 . AC=2;AF=0.0167;AN=120;BaseQRankSum=0.251;DP=2785;Dels=0.00;FS=2.468;HRun=0;HaplotypeScore=2.4818;InbreedingCoeff=-0.0179;MQ=58.39;MQ0=0;MQRankSum=2.076;QD=14.75;ReadPosRankSum=3.534;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|25),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 984957 . G A 998.45 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.493;DP=2152;Dels=0.00;FS=5.524;HRun=0;HaplotypeScore=1.2695;InbreedingCoeff=-0.0190;MQ=58.57;MQ0=0;MQRankSum=-0.059;QD=17.83;ReadPosRankSum=1.102;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGg/cAg|R1509Q|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|cGg/cAg|R/Q|ENSG00000188157|AGRN|ENST00000379370|26/36|possibly_damaging(0.459)|tolerated(0.3)|1509/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985165 . C T 53.15 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.519;DP=760;Dels=0.00;FS=6.936;HRun=0;HaplotypeScore=0.5648;InbreedingCoeff=-0.0414;MQ=58.09;MQ0=0;MQRankSum=0.442;QD=3.32;ReadPosRankSum=1.429;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P1578|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|ccC/ccT|P|ENSG00000188157|AGRN|ENST00000379370|26/36|||1578/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985239 . C T 170.83 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.190;DP=977;Dels=0.00;FS=9.028;HRun=0;HaplotypeScore=0.4363;InbreedingCoeff=-0.0324;MQ=58.40;MQ0=0;MQRankSum=-0.599;QD=6.57;ReadPosRankSum=0.946;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|26),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985266 . C T 19790.42 . AC=69;AF=0.5750;AN=120;BaseQRankSum=-0.605;DP=1126;Dels=0.00;FS=27.225;HRun=0;HaplotypeScore=0.6765;InbreedingCoeff=-0.0986;MQ=58.34;MQ0=0;MQRankSum=-0.994;QD=19.93;ReadPosRankSum=-1.430;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|26),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985378 . G A 349.18 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.347;DP=1151;Dels=0.00;FS=36.082;HRun=1;HaplotypeScore=0.7396;InbreedingCoeff=-0.0111;MQ=57.13;MQ0=0;MQRankSum=0.215;QD=14.55;ReadPosRankSum=1.577;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gag/Aag|E1614K|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|);CSQ=downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|Gag/Aag|E/K|ENSG00000188157|AGRN|ENST00000379370|27/36|benign(0.087)|tolerated(0.36)|1614/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985443 . AG A 5524.19 . AC=63;AF=0.5250;AN=120;BaseQRankSum=-4.267;DP=1211;FS=1.118;HRun=15;HaplotypeScore=183.6608;InbreedingCoeff=0.2307;MQ=52.69;MQ0=0;MQRankSum=2.703;QD=6.02;ReadPosRankSum=0.541;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985445 . G GT 4225.58 . AC=52;AF=0.4333;AN=120;BaseQRankSum=0.352;DP=1238;FS=80.878;HRun=0;HaplotypeScore=191.8295;InbreedingCoeff=-0.1480;MQ=52.62;MQ0=0;MQRankSum=2.617;QD=4.70;ReadPosRankSum=-5.379;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985446 . G T 10415.52 . AC=55;AF=0.4583;AN=120;BaseQRankSum=-15.323;DP=1238;Dels=0.00;FS=78.688;HRun=0;HaplotypeScore=12.5413;InbreedingCoeff=-0.0418;MQ=52.62;MQ0=0;MQRankSum=3.979;QD=11.59;ReadPosRankSum=-1.572;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985447 . G T 432.38 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-5.349;DP=1263;Dels=0.00;FS=3.447;HRun=0;HaplotypeScore=12.2057;InbreedingCoeff=-0.0488;MQ=52.38;MQ0=0;MQRankSum=-2.193;QD=3.90;ReadPosRankSum=0.844;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985449 . G A 14507.18 . AC=74;AF=0.6167;AN=120;BaseQRankSum=-12.718;DP=1283;Dels=0.00;FS=44.752;HRun=0;HaplotypeScore=13.5390;InbreedingCoeff=-0.3559;MQ=52.41;MQ0=0;MQRankSum=2.768;QD=12.78;ReadPosRankSum=-2.687;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985450 . G A 9694.98 . AC=38;AF=0.3167;AN=120;BaseQRankSum=-9.217;DP=1307;Dels=0.00;FS=25.729;HRun=0;HaplotypeScore=14.9791;InbreedingCoeff=-0.3897;MQ=52.45;MQ0=0;MQRankSum=4.412;QD=10.72;ReadPosRankSum=2.909;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985458 . GT G 311.7 . AC=16;AF=0.1333;AN=120;BaseQRankSum=4.206;DP=1448;FS=204.972;HRun=2;HaplotypeScore=201.5164;InbreedingCoeff=-0.1309;MQ=53.65;MQ0=0;MQRankSum=1.914;QD=0.64;ReadPosRankSum=10.293;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985460 . T C 3061.71 . AC=16;AF=0.1333;AN=120;BaseQRankSum=-0.490;DP=1456;Dels=0.00;FS=6.540;HRun=0;HaplotypeScore=4.9126;InbreedingCoeff=0.2588;MQ=53.76;MQ0=0;MQRankSum=-0.066;QD=11.78;ReadPosRankSum=0.826;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985509 . G A 2780.67 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.579;DP=2940;Dels=0.00;FS=10.079;HRun=0;HaplotypeScore=1.7811;InbreedingCoeff=-0.0096;MQ=56.77;MQ0=0;MQRankSum=2.214;QD=17.60;ReadPosRankSum=0.249;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|27),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985662 . C T 105.9 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-11.359;DP=3310;Dels=0.00;FS=122.177;HRun=2;HaplotypeScore=2.7454;InbreedingCoeff=-0.0439;MQ=57.76;MQ0=0;MQRankSum=-6.298;QD=0.47;ReadPosRankSum=-5.444;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F1643|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|ttC/ttT|F|ENSG00000188157|AGRN|ENST00000379370|28/36|||1643/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985797 . A G 944.49 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.428;DP=2302;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.3924;InbreedingCoeff=-0.0171;MQ=57.79;MQ0=0;MQRankSum=2.462;QD=13.12;ReadPosRankSum=0.537;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|28),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985826 . G A 438.23 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.507;DP=2255;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.1410;InbreedingCoeff=-0.0085;MQ=57.85;MQ0=0;MQRankSum=0.635;QD=14.61;ReadPosRankSum=-0.381;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtc/Atc|V1666I|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|Gtc/Atc|V/I|ENSG00000188157|AGRN|ENST00000379370|29/36|benign(0.119)|tolerated(0.44)|1666/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985855 . C T 288.16 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.233;DP=2094;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.0217;InbreedingCoeff=-0.0087;MQ=58.10;MQ0=0;MQRankSum=0.034;QD=11.53;ReadPosRankSum=-1.888;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G1675|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|ggC/ggT|G|ENSG00000188157|AGRN|ENST00000379370|29/36|||1675/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985900 . C T 558.67 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.271;DP=2177;Dels=0.00;FS=1.593;HRun=2;HaplotypeScore=1.0978;InbreedingCoeff=-0.0209;MQ=58.11;MQ0=0;MQRankSum=0.567;QD=10.16;ReadPosRankSum=-1.144;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),SYNONYMOUS_CODING(LOW|SILENT|ttC/ttT|F1690|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|ttC/ttT|F|ENSG00000188157|AGRN|ENST00000379370|29/36|||1690/2045|protein_coding|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 985999 . G T 316.5 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.550;DP=1428;Dels=0.00;FS=4.252;HRun=0;HaplotypeScore=0.7980;InbreedingCoeff=-0.0179;MQ=58.27;MQ0=0;MQRankSum=0.065;QD=8.79;ReadPosRankSum=0.661;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|29),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 986038 . G A 80.39 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.606;DP=1099;Dels=0.00;FS=1.750;HRun=0;HaplotypeScore=0.5655;InbreedingCoeff=-0.0135;MQ=57.65;MQ0=0;MQRankSum=0.062;QD=8.04;ReadPosRankSum=0.009;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|29),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 986285 . C T 224.77 . AC=2;AF=0.0169;AN=118;BaseQRankSum=0.797;DP=1956;Dels=0.00;FS=30.697;HRun=0;HaplotypeScore=1.3276;InbreedingCoeff=-0.0312;MQ=54.91;MQ0=0;MQRankSum=2.696;QD=12.49;ReadPosRankSum=-0.681;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|30),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 986286 . C T 649.39 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-0.566;DP=1981;Dels=0.00;FS=22.049;HRun=0;HaplotypeScore=1.6726;InbreedingCoeff=-0.0217;MQ=54.94;MQ0=0;MQRankSum=4.757;QD=11.39;ReadPosRankSum=1.099;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|30),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 986443 . C T 41215.18 . AC=100;AF=0.8621;AN=116;BaseQRankSum=0.781;DP=1363;Dels=0.00;FS=2.212;HRun=0;HaplotypeScore=0.8018;InbreedingCoeff=0.1240;MQ=57.18;MQ0=0;MQRankSum=-1.053;QD=31.27;ReadPosRankSum=-1.007;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000479707|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|30),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000479707|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 986737 . T C 414.03 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-2.032;DP=1137;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7186;InbreedingCoeff=-0.0327;MQ=58.21;MQ0=0;MQRankSum=1.287;QD=8.63;ReadPosRankSum=2.285;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),SYNONYMOUS_CODING(LOW|SILENT|ggT/ggC|G1786|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|ggT/ggC|G|ENSG00000188157|AGRN|ENST00000379370|31/36|||1786/2045|protein_coding|1,synonymous_variant|ggT/ggC|G|ENSG00000188157|AGRN|ENST00000419249|4/9|||89/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 986885 . T G 155.26 . AC=7;AF=0.0593;AN=118;BaseQRankSum=-10.562;DP=745;Dels=0.00;FS=53.331;HRun=2;HaplotypeScore=0.5798;InbreedingCoeff=-0.0971;MQ=57.41;MQ0=0;MQRankSum=1.830;QD=1.14;ReadPosRankSum=-2.492;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTg/gGg|V1808G|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,missense_variant|gTg/gGg|V/G|ENSG00000188157|AGRN|ENST00000379370|32/36|possibly_damaging(0.727)|tolerated(0.14)|1808/2045|protein_coding|1,missense_variant|gTg/gGg|V/G|ENSG00000188157|AGRN|ENST00000419249|5/9|possibly_damaging(0.723)|tolerated(0.1)|111/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 986920 . C A 169.9 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.934;DP=839;Dels=0.00;FS=1.422;HRun=0;HaplotypeScore=0.7669;InbreedingCoeff=-0.0288;MQ=58.26;MQ0=0;MQRankSum=0.709;QD=10.62;ReadPosRankSum=2.238;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),SYNONYMOUS_CODING(LOW|SILENT|Cgg/Agg|R1820|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,synonymous_variant|Cgg/Agg|R|ENSG00000188157|AGRN|ENST00000379370|32/36|||1820/2045|protein_coding|1,synonymous_variant|Cgg/Agg|R|ENSG00000188157|AGRN|ENST00000419249|5/9|||123/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 987200 . C T 92501.43 . AC=100;AF=0.8333;AN=120;BaseQRankSum=1.193;DP=3392;Dels=0.00;FS=27.772;HRun=0;HaplotypeScore=2.0645;InbreedingCoeff=0.1600;MQ=56.45;MQ0=0;MQRankSum=1.254;QD=28.89;ReadPosRankSum=-2.661;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|33),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 987253 . C T 635.19 . AC=4;AF=0.0333;AN=120;BaseQRankSum=0.926;DP=2121;Dels=0.00;FS=5.063;HRun=0;HaplotypeScore=1.9053;InbreedingCoeff=-0.0356;MQ=53.70;MQ0=0;MQRankSum=0.039;QD=7.22;ReadPosRankSum=-2.083;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|33),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 987311 . T C 44.81 . AC=8;AF=0.0667;AN=120;BaseQRankSum=-7.260;DP=1543;Dels=0.00;FS=87.443;HRun=3;HaplotypeScore=1.4824;InbreedingCoeff=0.0204;MQ=46.77;MQ0=0;MQRankSum=0.941;QD=0.41;ReadPosRankSum=-2.570;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|33),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 987341 . C T 217.82 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.265;DP=1310;Dels=0.00;FS=10.232;HRun=0;HaplotypeScore=1.1876;InbreedingCoeff=-0.0145;MQ=48.15;MQ0=0;MQRankSum=2.296;QD=7.03;ReadPosRankSum=0.839;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|33),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 987342 . G A 259.15 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-4.057;DP=1247;Dels=0.00;FS=1.217;HRun=0;HaplotypeScore=1.1837;InbreedingCoeff=-0.0234;MQ=48.33;MQ0=0;MQRankSum=1.716;QD=5.76;ReadPosRankSum=0.808;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|33),UPSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,upstream_gene_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 987436 . A G 63.69 . AC=9;AF=0.0865;AN=104;BaseQRankSum=2.297;DP=458;Dels=0.00;FS=20.190;HRun=0;HaplotypeScore=0.9717;InbreedingCoeff=-0.0835;MQ=56.48;MQ0=0;MQRankSum=-5.343;QD=0.49;ReadPosRankSum=-3.854;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|33),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 987670 . T G 1282.33 . AC=44;AF=0.846;AN=52;BaseQRankSum=-0.078;DP=47;Dels=0.00;FS=6.479;HRun=0;HaplotypeScore=0.0222;InbreedingCoeff=0.1213;MQ=59.01;MQ0=0;MQRankSum=-1.300;QD=30.53;ReadPosRankSum=0.391;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000466223|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000478677|),DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|33),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000466223|||||retained_intron|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000478677|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 988932 . G C 36554.37 . AC=79;AF=0.7596;AN=104;BaseQRankSum=-0.433;DP=1556;Dels=0.00;FS=2.118;HRun=3;HaplotypeScore=0.6684;InbreedingCoeff=0.1833;MQ=57.12;MQ0=0;MQRankSum=-1.108;QD=26.28;ReadPosRankSum=-0.844;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|1),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|33),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,intron_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 989423 . G A 1179.93 . AC=6;AF=0.0500;AN=120;BaseQRankSum=-0.586;DP=696;Dels=0.00;FS=1.039;HRun=0;HaplotypeScore=0.3917;InbreedingCoeff=0.5564;MQ=58.43;MQ0=0;MQRankSum=0.139;QD=23.14;ReadPosRankSum=-0.143;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|34),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|1),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 989461 . G A 47.93 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.647;DP=465;Dels=0.00;FS=5.710;HRun=0;HaplotypeScore=0.2114;InbreedingCoeff=-0.0294;MQ=58.14;MQ0=0;MQRankSum=-0.206;QD=5.99;ReadPosRankSum=1.402;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|34),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|1),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 989500 . G A 537.75 . AC=8;AF=0.0678;AN=118;BaseQRankSum=0.771;DP=317;Dels=0.00;FS=1.526;HRun=1;HaplotypeScore=0.4247;InbreedingCoeff=0.2291;MQ=57.86;MQ0=0;MQRankSum=1.486;QD=19.21;ReadPosRankSum=-0.692;EFF=DOWNSTREAM(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000492947|),INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|34),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|1),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000492947|||||retained_intron|1 -chr1 989811 . C A 1223.0 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-3.075;DP=1715;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.1951;InbreedingCoeff=-0.0226;MQ=58.98;MQ0=0;MQRankSum=0.887;QD=11.87;ReadPosRankSum=-0.484;EFF=INTRON(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|34),INTRON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|1),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,intron_variant|||ENSG00000188157|AGRN|ENST00000379370||||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|||||retained_intron|1 -chr1 989847 . C T 704.05 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.110;DP=2200;Dels=0.00;FS=6.860;HRun=1;HaplotypeScore=1.7382;InbreedingCoeff=-0.0131;MQ=58.83;MQ0=0;MQRankSum=-0.133;QD=10.67;ReadPosRankSum=0.559;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|2),SYNONYMOUS_CODING(LOW|SILENT|Ctg/Ttg|L1966|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,synonymous_variant|Ctg/Ttg|L|ENSG00000188157|AGRN|ENST00000379370|35/36|||1966/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|2/3||||retained_intron|1 -chr1 990213 . C T 965.08 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.966;DP=1603;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.3432;InbreedingCoeff=-0.0258;MQ=58.45;MQ0=0;MQRankSum=0.239;QD=15.57;ReadPosRankSum=-0.735;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P1997L|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,missense_variant|cCg/cTg|P/L|ENSG00000188157|AGRN|ENST00000379370|36/36|possibly_damaging(0.612)|deleterious(0.04)|1997/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 -chr1 990280 . C T 40484.94 . AC=89;AF=0.7672;AN=116;BaseQRankSum=-1.914;DP=1861;Dels=0.00;FS=0.408;HRun=0;HaplotypeScore=1.6608;InbreedingCoeff=-0.0422;MQ=58.21;MQ0=0;MQRankSum=1.964;QD=23.35;ReadPosRankSum=-2.688;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D2019|2045|AGRN|protein_coding|CODING|ENST00000379370|),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,synonymous_variant|gaC/gaT|D|ENSG00000188157|AGRN|ENST00000379370|36/36|||2019/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 -chr1 990380 . C T 8429.21 . AC=25;AF=0.2119;AN=118;BaseQRankSum=-1.731;DP=1638;Dels=0.00;FS=19.219;HRun=0;HaplotypeScore=0.9542;InbreedingCoeff=0.0822;MQ=58.01;MQ0=0;MQRankSum=-0.121;QD=14.79;ReadPosRankSum=1.180;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UPSTREAM(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=upstream_gene_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 -chr1 990417 . T C 8344.97 . AC=27;AF=0.2328;AN=116;BaseQRankSum=-1.168;DP=1150;Dels=0.00;FS=1.555;HRun=0;HaplotypeScore=0.4386;InbreedingCoeff=0.0986;MQ=57.95;MQ0=0;MQRankSum=-0.663;QD=16.33;ReadPosRankSum=-1.059;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),EXON(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|1),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|1/2||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 -chr1 990517 . C T 9650.24 . AC=75;AF=0.798;AN=94;BaseQRankSum=1.624;DP=348;Dels=0.00;FS=0.641;HRun=0;HaplotypeScore=0.4773;InbreedingCoeff=0.1734;MQ=57.98;MQ0=0;MQRankSum=0.522;QD=30.54;ReadPosRankSum=1.100;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),EXON(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|1),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|1/2||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 -chr1 990557 . C T 59.21 . AC=1;AF=0.011;AN=90;BaseQRankSum=2.271;DP=279;Dels=0.00;FS=2.553;HRun=0;HaplotypeScore=0.3165;InbreedingCoeff=-0.0942;MQ=57.30;MQ0=0;MQRankSum=0.765;QD=8.46;ReadPosRankSum=-0.239;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),EXON(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|1),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|1/2||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 -chr1 990773 . C T 25625.67 . AC=51;AF=0.689;AN=74;BaseQRankSum=-0.671;DP=1023;Dels=0.00;FS=8.374;HRun=0;HaplotypeScore=0.7685;InbreedingCoeff=0.2919;MQ=57.82;MQ0=0;MQRankSum=0.992;QD=27.26;ReadPosRankSum=1.535;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),INTRON(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|1),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=intron_variant&nc_transcript_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 -chr1 990806 . G A 25266.48 . AC=55;AF=0.764;AN=72;BaseQRankSum=-2.112;DP=1071;Dels=0.00;FS=2.830;HRun=0;HaplotypeScore=0.8208;InbreedingCoeff=0.2300;MQ=58.13;MQ0=0;MQRankSum=-0.461;QD=25.63;ReadPosRankSum=0.166;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),INTRON(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|1),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=intron_variant&nc_transcript_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 -chr1 990839 . C T 1668.45 . AC=2;AF=0.029;AN=70;BaseQRankSum=1.196;DP=1096;Dels=0.00;FS=1.633;HRun=2;HaplotypeScore=0.6892;InbreedingCoeff=-0.0818;MQ=58.31;MQ0=0;MQRankSum=1.390;QD=12.27;ReadPosRankSum=-2.018;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),INTRON(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|1),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=intron_variant&nc_transcript_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 -chr1 990943 . G C 216.41 . AC=1;AF=0.019;AN=52;BaseQRankSum=-0.081;DP=536;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6541;InbreedingCoeff=-0.0441;MQ=58.64;MQ0=0;MQRankSum=-0.296;QD=10.31;ReadPosRankSum=-0.366;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),INTRON(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|1),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=intron_variant&nc_transcript_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 -chr1 990984 . G A 3835.45 . AC=33;AF=0.786;AN=42;BaseQRankSum=-0.791;DP=131;Dels=0.00;FS=7.195;HRun=0;HaplotypeScore=0.1328;InbreedingCoeff=0.2454;MQ=58.69;MQ0=0;MQRankSum=-2.076;QD=32.78;ReadPosRankSum=-1.157;EFF=EXON(MODIFIER|||||AGRN|retained_intron|CODING|ENST00000461111|3),INTRON(MODIFIER|||||RP11-54O7.14|processed_transcript|NON_CODING|ENST00000418300|1),UPSTREAM(MODIFIER|||||RP11-465B22.3|processed_transcript|NON_CODING|ENST00000427998|),UTR_3_PRIME(MODIFIER||||2045|AGRN|protein_coding|CODING|ENST00000379370|36);CSQ=intron_variant&nc_transcript_variant|||ENSG00000242590|RP11-54O7.14|ENST00000418300|||||sense_intronic|1,3_prime_UTR_variant|||ENSG00000188157|AGRN|ENST00000379370|36/36|||-/2045|protein_coding|1,downstream_gene_variant|||ENSG00000188157|AGRN|ENST00000419249||||-/271|protein_coding|1,upstream_gene_variant|||ENSG00000217801|RP11-465B22.3|ENST00000427998|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000188157|AGRN|ENST00000461111|3/3||||retained_intron|1 -chr1 1102069 . A G 744.18 . AC=36;AF=0.900;AN=40;BaseQRankSum=-0.783;DP=29;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0800;InbreedingCoeff=0.1040;MQ=56.74;MQ0=0;MQRankSum=0.679;QD=32.36;ReadPosRankSum=1.410;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=upstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1102296 . G A 783.47 . AC=4;AF=0.0357;AN=112;BaseQRankSum=-0.578;DP=498;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.2286;InbreedingCoeff=0.2474;MQ=55.59;MQ0=0;MQRankSum=0.771;QD=18.22;ReadPosRankSum=0.212;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=upstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1102360 . T G 216.82 . AC=16;AF=0.1379;AN=116;BaseQRankSum=-10.289;DP=1092;Dels=0.00;FS=25.292;HRun=4;HaplotypeScore=1.6139;InbreedingCoeff=-0.2074;MQ=53.49;MQ0=0;MQRankSum=2.414;QD=0.71;ReadPosRankSum=7.754;EFF=INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=upstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1102563 . G A 1052.38 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.152;DP=4513;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=2.2999;InbreedingCoeff=-0.0179;MQ=56.60;MQ0=0;MQRankSum=2.502;QD=14.03;ReadPosRankSum=-0.938;EFF=EXON(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|1),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000207730|MIR200B|ENST00000384997|1/1||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1102657 . C T 464.41 . AC=2;AF=0.0169;AN=118;BaseQRankSum=0.429;DP=1409;Dels=0.00;FS=2.023;HRun=0;HaplotypeScore=1.5640;InbreedingCoeff=-0.0230;MQ=53.83;MQ0=0;MQRankSum=0.937;QD=12.90;ReadPosRankSum=0.639;EFF=DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1102674 . G A 964.97 . AC=2;AF=0.0172;AN=116;BaseQRankSum=0.337;DP=1131;Dels=0.00;FS=1.614;HRun=0;HaplotypeScore=1.4083;InbreedingCoeff=-0.0229;MQ=55.34;MQ0=0;MQRankSum=1.906;QD=12.21;ReadPosRankSum=1.395;EFF=DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1102717 . C T 175.26 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.432;DP=649;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.6822;InbreedingCoeff=-0.0345;MQ=56.83;MQ0=0;MQRankSum=1.021;QD=9.22;ReadPosRankSum=1.763;EFF=DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1103049 . C T 201.32 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.756;DP=465;Dels=0.00;FS=3.302;HRun=0;HaplotypeScore=0.3840;InbreedingCoeff=-0.0481;MQ=57.23;MQ0=0;MQRankSum=0.101;QD=9.15;ReadPosRankSum=-0.265;EFF=DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1103150 . G A 13784.17 . AC=26;AF=0.2281;AN=114;BaseQRankSum=2.288;DP=1923;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.8331;InbreedingCoeff=0.0633;MQ=56.95;MQ0=0;MQRankSum=6.711;QD=19.50;ReadPosRankSum=1.282;EFF=DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1103231 . C G 920.65 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.613;DP=4071;Dels=0.00;FS=1.295;HRun=0;HaplotypeScore=2.5754;InbreedingCoeff=-0.0143;MQ=57.14;MQ0=0;MQRankSum=0.020;QD=9.59;ReadPosRankSum=1.029;EFF=DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,upstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1103423 . GA G 310.03 . AC=11;AF=0.0932;AN=118;BaseQRankSum=3.186;DP=1367;FS=7.032;HRun=1;HaplotypeScore=112.5777;InbreedingCoeff=-0.1319;MQ=56.78;MQ0=0;MQRankSum=-1.054;QD=1.13;ReadPosRankSum=-12.462;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1103424 . A G 291.86 . AC=11;AF=0.0932;AN=118;BaseQRankSum=-4.363;DP=1367;Dels=0.01;FS=12.595;HRun=3;HaplotypeScore=1.1874;InbreedingCoeff=-0.1339;MQ=56.78;MQ0=0;MQRankSum=-0.884;QD=0.96;ReadPosRankSum=-10.797;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1103436 . C T 253.89 . AC=1;AF=0.0094;AN=106;BaseQRankSum=1.346;DP=1176;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.0420;InbreedingCoeff=-0.0281;MQ=56.38;MQ0=0;MQRankSum=-1.314;QD=7.25;ReadPosRankSum=-3.184;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1103471 . C T 3129.31 . AC=41;AF=0.3596;AN=114;BaseQRankSum=7.298;DP=802;Dels=0.00;FS=25.877;HRun=0;HaplotypeScore=0.4705;InbreedingCoeff=-0.6199;MQ=56.31;MQ0=0;MQRankSum=4.884;QD=4.53;ReadPosRankSum=-15.448;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1103479 . G C 231.39 . AC=9;AF=0.0804;AN=112;BaseQRankSum=-1.555;DP=715;Dels=0.00;FS=4.645;HRun=2;HaplotypeScore=0.4522;InbreedingCoeff=-0.1256;MQ=56.12;MQ0=0;MQRankSum=1.010;QD=2.22;ReadPosRankSum=-8.435;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1103537 . C T 42.98 . AC=4;AF=0.0364;AN=110;BaseQRankSum=-0.058;DP=526;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3527;InbreedingCoeff=0.3068;MQ=56.99;MQ0=0;MQRankSum=1.015;QD=14.33;ReadPosRankSum=0.313;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1103542 . T C 13387.36 . AC=101;AF=0.9182;AN=110;BaseQRankSum=2.524;DP=466;Dels=0.00;FS=6.051;HRun=2;HaplotypeScore=0.2267;InbreedingCoeff=0.0050;MQ=57.11;MQ0=0;MQRankSum=1.977;QD=29.29;ReadPosRankSum=-2.662;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1103690 . T TCA 4213.79 . AC=60;AF=0.909;AN=66;BaseQRankSum=-2.219;DP=87;FS=4.960;HRun=0;HaplotypeScore=8.2650;InbreedingCoeff=0.2982;MQ=60.22;MQ0=0;MQRankSum=-0.386;QD=52.02;ReadPosRankSum=-1.623;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1103958 . T G 972.47 . AC=38;AF=0.905;AN=42;BaseQRankSum=0.039;DP=33;Dels=0.00;FS=2.722;HRun=0;HaplotypeScore=0.0376;InbreedingCoeff=0.1168;MQ=59.30;MQ0=0;MQRankSum=0.973;QD=31.37;ReadPosRankSum=-0.195;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1 -chr1 1104406 . C CCAGA 1690.42 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-4.911;DP=3757;FS=4.900;HRun=0;HaplotypeScore=321.6033;InbreedingCoeff=-0.0086;MQ=57.98;MQ0=0;MQRankSum=-8.640;QD=27.71;ReadPosRankSum=0.563;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),EXON(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|1),UPSTREAM(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|),UPSTREAM(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant&feature_elongation|||ENSG00000198976|MIR429|ENST00000362106|1/1||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1 -chr1 1104418 . A C 1306.94 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.348;DP=3836;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.7746;InbreedingCoeff=-0.0084;MQ=57.98;MQ0=0;MQRankSum=-0.429;QD=15.38;ReadPosRankSum=1.041;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),EXON(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|1),UPSTREAM(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|),UPSTREAM(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000198976|MIR429|ENST00000362106|1/1||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1 -chr1 1104584 . T C 2225.46 . AC=7;AF=0.0583;AN=120;BaseQRankSum=-1.668;DP=1142;Dels=0.00;FS=2.698;HRun=0;HaplotypeScore=0.5785;InbreedingCoeff=0.5329;MQ=58.24;MQ0=0;MQRankSum=1.566;QD=23.93;ReadPosRankSum=1.120;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|),UPSTREAM(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1,downstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1 -chr1 1104624 . C T 353.09 . AC=2;AF=0.0167;AN=120;BaseQRankSum=1.668;DP=653;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.6949;InbreedingCoeff=-0.0240;MQ=57.96;MQ0=0;MQRankSum=0.018;QD=15.35;ReadPosRankSum=0.448;EFF=DOWNSTREAM(MODIFIER|||||MIR200A|miRNA|NON_CODING|ENST00000384875|),DOWNSTREAM(MODIFIER|||||MIR200B|miRNA|NON_CODING|ENST00000384997|),DOWNSTREAM(MODIFIER|||||MIR429|miRNA|NON_CODING|ENST00000362106|),DOWNSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|),UPSTREAM(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|);CSQ=downstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,downstream_gene_variant|||ENSG00000207730|MIR200B|ENST00000384997|||||miRNA|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1,downstream_gene_variant|||ENSG00000198976|MIR429|ENST00000362106|||||miRNA|1,downstream_gene_variant|||ENSG00000207607|MIR200A|ENST00000384875|||||miRNA|1,upstream_gene_variant|||ENSG00000272141|RP11-465B22.8|ENST00000606993|||||lincRNA|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1 -chr1 1115086 . C T 1455.37 . AC=1;AF=0.0086;AN=116;BaseQRankSum=-2.311;DP=1823;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.0613;InbreedingCoeff=-0.0319;MQ=58.20;MQ0=0;MQRankSum=0.635;QD=14.13;ReadPosRankSum=-1.886;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G56|673|TTLL10|protein_coding|CODING|ENST00000379289|),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G56|673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),UTR_5_PRIME(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|1);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,synonymous_variant|ggC/ggT|G|ENSG00000162571|TTLL10|ENST00000379289|5/16|||56/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,synonymous_variant|ggC/ggT|G|ENSG00000162571|TTLL10|ENST00000379290|5/16|||56/673|protein_coding|1,5_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|1/9|||-/404|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1 -chr1 1115187 . C T 128.35 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.636;DP=678;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5936;InbreedingCoeff=-0.0317;MQ=57.89;MQ0=0;MQRankSum=0.815;QD=14.26;ReadPosRankSum=0.228;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|5),START_GAINED(LOW||||404|TTLL10|protein_coding|CODING|ENST00000379288|1),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,5_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|1/9|||-/404|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1 -chr1 1115210 . G A 3468.51 . AC=23;AF=0.1983;AN=116;BaseQRankSum=-0.114;DP=539;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6710;InbreedingCoeff=0.0851;MQ=58.05;MQ0=0;MQRankSum=-0.518;QD=16.84;ReadPosRankSum=-1.559;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|5),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),UTR_5_PRIME(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|1);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,5_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|1/9|||-/404|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1 -chr1 1115213 . G T 2687.22 . AC=21;AF=0.1810;AN=116;BaseQRankSum=0.184;DP=530;Dels=0.00;FS=4.737;HRun=1;HaplotypeScore=0.7398;InbreedingCoeff=0.1254;MQ=58.19;MQ0=0;MQRankSum=0.107;QD=15.10;ReadPosRankSum=-1.774;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|5),START_GAINED(LOW||||404|TTLL10|protein_coding|CODING|ENST00000379288|1),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,5_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|1/9|||-/404|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1 -chr1 1115222 . C T 45.39 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-0.693;DP=471;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3240;InbreedingCoeff=-0.0359;MQ=58.17;MQ0=0;MQRankSum=0.038;QD=5.67;ReadPosRankSum=-1.442;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|5),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),UTR_5_PRIME(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|1);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,5_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|1/9|||-/404|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1 -chr1 1115379 . G A 616.46 . AC=5;AF=0.0417;AN=120;BaseQRankSum=0.553;DP=584;Dels=0.00;FS=2.233;HRun=0;HaplotypeScore=0.3425;InbreedingCoeff=-0.0782;MQ=58.20;MQ0=0;MQRankSum=1.750;QD=9.94;ReadPosRankSum=0.385;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|processed_transcript|CODING|ENST00000506177|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|1),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|5),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000506177|||||processed_transcript|1 -chr1 1115415 . C T 177.42 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-1.248;DP=752;Dels=0.00;FS=3.560;HRun=0;HaplotypeScore=0.6217;InbreedingCoeff=-0.0287;MQ=58.39;MQ0=0;MQRankSum=1.308;QD=8.06;ReadPosRankSum=-0.853;EFF=SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G67|673|TTLL10|protein_coding|CODING|ENST00000379289|),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G67|673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),UTR_5_PRIME(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|2);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,splice_region_variant&synonymous_variant|ggC/ggT|G|ENSG00000162571|TTLL10|ENST00000379289|6/16|||67/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,splice_region_variant&synonymous_variant|ggC/ggT|G|ENSG00000162571|TTLL10|ENST00000379290|6/16|||67/673|protein_coding|1,splice_region_variant&5_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|2/9|||-/404|protein_coding|1 -chr1 1115461 . C T 331.7 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.166;DP=949;Dels=0.00;FS=1.152;HRun=0;HaplotypeScore=0.9760;InbreedingCoeff=-0.0264;MQ=58.65;MQ0=0;MQRankSum=1.177;QD=12.76;ReadPosRankSum=1.958;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgg/Tgg|R10W|404|TTLL10|protein_coding|CODING|ENST00000379288|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgg/Tgg|R83W|673|TTLL10|protein_coding|CODING|ENST00000379289|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgg/Tgg|R83W|673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,missense_variant|Cgg/Tgg|R/W|ENSG00000162571|TTLL10|ENST00000379289|6/16|benign(0.28)|tolerated(0.08)|83/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,missense_variant|Cgg/Tgg|R/W|ENSG00000162571|TTLL10|ENST00000379290|6/16|benign(0.28)|tolerated(0.08)|83/673|protein_coding|1,missense_variant|Cgg/Tgg|R/W|ENSG00000162571|TTLL10|ENST00000379288|2/9|benign(0.365)|deleterious(0.03)|10/404|protein_coding|1 -chr1 1115503 . T C 1171.08 . AC=4;AF=0.0333;AN=120;BaseQRankSum=1.533;DP=1103;Dels=0.00;FS=7.100;HRun=1;HaplotypeScore=0.5750;InbreedingCoeff=-0.0426;MQ=59.00;MQ0=0;MQRankSum=1.371;QD=12.59;ReadPosRankSum=-1.312;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tgt/Cgt|C24R|404|TTLL10|protein_coding|CODING|ENST00000379288|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tgt/Cgt|C97R|673|TTLL10|protein_coding|CODING|ENST00000379289|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tgt/Cgt|C97R|673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,missense_variant|Tgt/Cgt|C/R|ENSG00000162571|TTLL10|ENST00000379289|6/16|benign(0.004)|tolerated(0.53)|97/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,missense_variant|Tgt/Cgt|C/R|ENSG00000162571|TTLL10|ENST00000379290|6/16|benign(0.004)|tolerated(0.53)|97/673|protein_coding|1,missense_variant|Tgt/Cgt|C/R|ENSG00000162571|TTLL10|ENST00000379288|2/9|benign(0.016)|tolerated(0.27)|24/404|protein_coding|1 -chr1 1115601 . C T 368.82 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-0.437;DP=1078;Dels=0.00;FS=12.205;HRun=0;HaplotypeScore=0.6665;InbreedingCoeff=-0.0165;MQ=58.97;MQ0=0;MQRankSum=0.473;QD=12.29;ReadPosRankSum=2.503;EFF=SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N129|673|TTLL10|protein_coding|CODING|ENST00000379289|),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N129|673|TTLL10|protein_coding|CODING|ENST00000379290|),SYNONYMOUS_CODING(LOW|SILENT|aaC/aaT|N56|404|TTLL10|protein_coding|CODING|ENST00000379288|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,synonymous_variant|aaC/aaT|N|ENSG00000162571|TTLL10|ENST00000379289|6/16|||129/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,synonymous_variant|aaC/aaT|N|ENSG00000162571|TTLL10|ENST00000379290|6/16|||129/673|protein_coding|1,synonymous_variant|aaC/aaT|N|ENSG00000162571|TTLL10|ENST00000379288|2/9|||56/404|protein_coding|1 -chr1 1115605 . G T 78.84 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.557;DP=1031;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6145;InbreedingCoeff=-0.0171;MQ=58.95;MQ0=0;MQRankSum=0.835;QD=4.93;ReadPosRankSum=0.136;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Tcc|A131S|673|TTLL10|protein_coding|CODING|ENST00000379289|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Tcc|A131S|673|TTLL10|protein_coding|CODING|ENST00000379290|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Tcc|A58S|404|TTLL10|protein_coding|CODING|ENST00000379288|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,missense_variant|Gcc/Tcc|A/S|ENSG00000162571|TTLL10|ENST00000379289|6/16|benign(0.021)|tolerated(0.07)|131/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,missense_variant|Gcc/Tcc|A/S|ENSG00000162571|TTLL10|ENST00000379290|6/16|benign(0.021)|tolerated(0.07)|131/673|protein_coding|1,missense_variant|Gcc/Tcc|A/S|ENSG00000162571|TTLL10|ENST00000379288|2/9|benign(0.087)|tolerated(0.07)|58/404|protein_coding|1 -chr1 1115739 . C T 563.52 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-0.563;DP=900;Dels=0.00;FS=2.949;HRun=0;HaplotypeScore=0.6729;InbreedingCoeff=-0.0307;MQ=58.26;MQ0=0;MQRankSum=1.624;QD=17.61;ReadPosRankSum=1.518;EFF=INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|2),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|6),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|6),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1115840 . AC A 723.63 . AC=2;AF=0.0175;AN=114;BaseQRankSum=1.500;DP=1665;FS=3.486;HRun=5;HaplotypeScore=137.9849;InbreedingCoeff=-0.0404;MQ=57.81;MQ0=0;MQRankSum=1.153;QD=10.19;ReadPosRankSum=1.601;EFF=INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|2),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|6),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|6),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),UPSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1115994 . G A 10204.82 . AC=24;AF=0.2000;AN=120;BaseQRankSum=-0.160;DP=1696;Dels=0.00;FS=0.223;HRun=0;HaplotypeScore=1.0682;InbreedingCoeff=0.0308;MQ=58.03;MQ0=0;MQRankSum=0.094;QD=16.57;ReadPosRankSum=1.260;EFF=EXON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|1),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|3),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|7),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|7),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|1),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|1/5||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1116005 . C T 537.05 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.435;DP=1666;Dels=0.00;FS=14.791;HRun=0;HaplotypeScore=1.1478;InbreedingCoeff=-0.0145;MQ=58.06;MQ0=0;MQRankSum=1.799;QD=13.43;ReadPosRankSum=-0.495;EFF=EXON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|1),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|3),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|7),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|7),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|1),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|1/5||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1116231 . T C 4814.31 . AC=14;AF=0.1167;AN=120;BaseQRankSum=2.445;DP=1389;Dels=0.00;FS=6.355;HRun=2;HaplotypeScore=1.7202;InbreedingCoeff=0.1639;MQ=56.15;MQ0=0;MQRankSum=1.206;QD=15.28;ReadPosRankSum=0.519;EFF=EXON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|1),EXON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|2),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTc/gCc|V176A|404|TTLL10|protein_coding|CODING|ENST00000379288|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTc/gCc|V249A|673|TTLL10|protein_coding|CODING|ENST00000379289|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTc/gCc|V249A|673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,missense_variant|gTc/gCc|V/A|ENSG00000162571|TTLL10|ENST00000379289|8/16|benign(0)|tolerated(1)|249/673|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|1/5||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000514695|2/2||||retained_intron|1,missense_variant|gTc/gCc|V/A|ENSG00000162571|TTLL10|ENST00000379290|8/16|benign(0)|tolerated(1)|249/673|protein_coding|1,missense_variant|gTc/gCc|V/A|ENSG00000162571|TTLL10|ENST00000379288|4/9|benign(0)|tolerated(1)|176/404|protein_coding|1 -chr1 1116311 . A G 3493.8 . AC=48;AF=0.4706;AN=102;BaseQRankSum=7.547;DP=286;Dels=0.01;FS=2.211;HRun=0;HaplotypeScore=0.9639;InbreedingCoeff=0.0983;MQ=52.73;MQ0=0;MQRankSum=0.694;QD=18.89;ReadPosRankSum=0.096;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|4),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|8),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|1),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1117111 . T C 797.3 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.679;DP=2103;Dels=0.00;FS=1.613;HRun=4;HaplotypeScore=1.6462;InbreedingCoeff=-0.0099;MQ=57.76;MQ0=0;MQRankSum=1.258;QD=16.61;ReadPosRankSum=-0.652;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|4),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|8),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|1),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1117200 . A ACTCAG 2202.05 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-4.680;DP=3092;FS=21.350;HRun=0;HaplotypeScore=287.1827;InbreedingCoeff=-0.0395;MQ=57.35;MQ0=0;MQRankSum=-7.883;QD=28.60;ReadPosRankSum=-0.894;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|9),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|9),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|2),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,splice_region_variant&intron_variant&feature_elongation|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,splice_region_variant&intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,splice_region_variant&intron_variant&feature_elongation|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,splice_region_variant&intron_variant&feature_elongation|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1117398 . A G 8833.57 . AC=60;AF=0.5172;AN=116;BaseQRankSum=3.163;DP=557;Dels=0.00;FS=2.617;HRun=0;HaplotypeScore=0.4621;InbreedingCoeff=0.0835;MQ=57.86;MQ0=0;MQRankSum=1.868;QD=22.25;ReadPosRankSum=0.084;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|9),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|9),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|2),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1117486 . A G 1272.34 . AC=17;AF=0.1441;AN=118;BaseQRankSum=-1.118;DP=292;Dels=0.00;FS=2.091;HRun=0;HaplotypeScore=0.1964;InbreedingCoeff=-0.1365;MQ=57.43;MQ0=0;MQRankSum=0.534;QD=14.14;ReadPosRankSum=0.569;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|9),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|9),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|2),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1117529 . A C 317.04 . AC=5;AF=0.0424;AN=118;BaseQRankSum=-1.360;DP=362;Dels=0.00;FS=1.548;HRun=1;HaplotypeScore=0.4341;InbreedingCoeff=-0.0846;MQ=57.60;MQ0=0;MQRankSum=0.707;QD=9.61;ReadPosRankSum=0.810;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|9),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|9),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|2),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1117709 . G A 2986.58 . AC=4;AF=0.0333;AN=120;BaseQRankSum=1.592;DP=3059;Dels=0.00;FS=8.817;HRun=1;HaplotypeScore=1.5659;InbreedingCoeff=-0.0345;MQ=59.00;MQ0=0;MQRankSum=-1.150;QD=12.82;ReadPosRankSum=-4.568;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|5),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|9),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|9),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|2),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1117795 . C G 1423.49 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.182;DP=4738;Dels=0.00;FS=1.073;HRun=1;HaplotypeScore=2.2289;InbreedingCoeff=-0.0084;MQ=59.08;MQ0=0;MQRankSum=-0.250;QD=14.53;ReadPosRankSum=2.714;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),EXON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|3),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|caC/caG|H222Q|404|TTLL10|protein_coding|CODING|ENST00000379288|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|caC/caG|H295Q|673|TTLL10|protein_coding|CODING|ENST00000379289|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|caC/caG|H295Q|673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,missense_variant|caC/caG|H/Q|ENSG00000162571|TTLL10|ENST00000379289|10/16|benign(0.046)|tolerated(0.21)|295/673|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|3/5||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,missense_variant|caC/caG|H/Q|ENSG00000162571|TTLL10|ENST00000379290|10/16|benign(0.046)|tolerated(0.21)|295/673|protein_coding|1,missense_variant|caC/caG|H/Q|ENSG00000162571|TTLL10|ENST00000379288|6/9|benign(0.085)|tolerated(0.23)|222/404|protein_coding|1 -chr1 1118119 . G A 94.5 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.954;DP=605;Dels=0.00;FS=6.112;HRun=0;HaplotypeScore=0.5514;InbreedingCoeff=-0.0360;MQ=57.50;MQ0=0;MQRankSum=0.026;QD=15.75;ReadPosRankSum=-0.231;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|6),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|10),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|10),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|3),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1118157 . C T 629.37 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.602;DP=775;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.4619;InbreedingCoeff=-0.0286;MQ=57.49;MQ0=0;MQRankSum=1.353;QD=15.73;ReadPosRankSum=-1.120;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|6),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|10),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|10),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|3),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1118212 . T C 16130.03 . AC=48;AF=0.4068;AN=118;BaseQRankSum=1.657;DP=1395;Dels=0.00;FS=12.889;HRun=1;HaplotypeScore=1.7343;InbreedingCoeff=0.1338;MQ=57.23;MQ0=0;MQRankSum=3.897;QD=20.34;ReadPosRankSum=-0.066;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|6),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|10),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|10),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|3),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1118244 . C T 1034.91 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.695;DP=2048;Dels=0.00;FS=2.342;HRun=0;HaplotypeScore=1.2560;InbreedingCoeff=-0.0165;MQ=57.74;MQ0=0;MQRankSum=0.426;QD=10.78;ReadPosRankSum=-0.861;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|6),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|10),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|10),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|3),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1118275 . C T 6047.77 . AC=9;AF=0.0750;AN=120;BaseQRankSum=-1.144;DP=2721;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.3228;InbreedingCoeff=-0.0826;MQ=57.77;MQ0=0;MQRankSum=4.599;QD=12.68;ReadPosRankSum=1.658;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),EXON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|4),SYNONYMOUS_CODING(LOW|SILENT|tgC/tgT|C239|404|TTLL10|protein_coding|CODING|ENST00000379288|),SYNONYMOUS_CODING(LOW|SILENT|tgC/tgT|C312|673|TTLL10|protein_coding|CODING|ENST00000379289|),SYNONYMOUS_CODING(LOW|SILENT|tgC/tgT|C312|673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,synonymous_variant|tgC/tgT|C|ENSG00000162571|TTLL10|ENST00000379289|11/16|||312/673|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|4/5||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,synonymous_variant|tgC/tgT|C|ENSG00000162571|TTLL10|ENST00000379290|11/16|||312/673|protein_coding|1,synonymous_variant|tgC/tgT|C|ENSG00000162571|TTLL10|ENST00000379288|7/9|||239/404|protein_coding|1 -chr1 1118491 . A G 315.28 . AC=4;AF=0.0339;AN=118;BaseQRankSum=-1.758;DP=1080;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=1.0651;InbreedingCoeff=-0.0464;MQ=56.58;MQ0=0;MQRankSum=0.706;QD=10.87;ReadPosRankSum=0.829;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|7),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|11),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|11),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|4),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1118522 . G A 231.29 . AC=2;AF=0.0169;AN=118;BaseQRankSum=0.115;DP=734;Dels=0.00;FS=22.046;HRun=0;HaplotypeScore=0.5763;InbreedingCoeff=-0.0479;MQ=55.98;MQ0=0;MQRankSum=0.916;QD=10.51;ReadPosRankSum=-2.177;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|7),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|11),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|11),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|4),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1118529 . G C 35.12 . AC=5;AF=0.0431;AN=116;BaseQRankSum=-3.621;DP=699;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.6631;InbreedingCoeff=0.1223;MQ=55.74;MQ0=0;MQRankSum=-0.031;QD=1.60;ReadPosRankSum=-2.064;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|7),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|11),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|11),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|4),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1118560 . C T 120.5 . AC=4;AF=0.0339;AN=118;BaseQRankSum=0.441;DP=502;Dels=0.00;FS=2.806;HRun=0;HaplotypeScore=0.5435;InbreedingCoeff=0.0953;MQ=53.92;MQ0=0;MQRankSum=2.564;QD=9.27;ReadPosRankSum=-0.278;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|7),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|11),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|11),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|4),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1118578 . G A 921.34 . AC=9;AF=0.0804;AN=112;BaseQRankSum=-0.517;DP=400;Dels=0.00;FS=2.546;HRun=1;HaplotypeScore=0.3066;InbreedingCoeff=-0.0266;MQ=53.56;MQ0=0;MQRankSum=0.375;QD=14.86;ReadPosRankSum=2.525;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|7),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|11),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|11),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|4),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1119080 . T C 90.74 . AC=1;AF=0.0086;AN=116;BaseQRankSum=0.742;DP=369;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.3252;InbreedingCoeff=-0.0596;MQ=58.29;MQ0=0;MQRankSum=0.380;QD=12.96;ReadPosRankSum=-0.100;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|7),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|11),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|11),INTRON(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|4),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1119399 . C T 1267.07 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.885;DP=8859;DS;Dels=0.00;FS=6.806;HRun=0;HaplotypeScore=4.5609;InbreedingCoeff=-0.0084;MQ=59.04;MQ0=0;MQRankSum=-1.312;QD=11.73;ReadPosRankSum=1.342;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H323|404|TTLL10|protein_coding|CODING|ENST00000379288|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H396|673|TTLL10|protein_coding|CODING|ENST00000379289|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H396|673|TTLL10|protein_coding|CODING|ENST00000379290|),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,synonymous_variant|caC/caT|H|ENSG00000162571|TTLL10|ENST00000379289|12/16|||396/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,synonymous_variant|caC/caT|H|ENSG00000162571|TTLL10|ENST00000379290|12/16|||396/673|protein_coding|1,synonymous_variant|caC/caT|H|ENSG00000162571|TTLL10|ENST00000379288|8/9|||323/404|protein_coding|1 -chr1 1119543 . G C 392.87 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.504;DP=2292;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.8936;InbreedingCoeff=-0.0096;MQ=59.06;MQ0=0;MQRankSum=0.601;QD=13.55;ReadPosRankSum=-1.427;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1119546 . G T 1327.72 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.808;DP=2166;Dels=0.00;FS=1.426;HRun=1;HaplotypeScore=1.8266;InbreedingCoeff=-0.0272;MQ=59.08;MQ0=0;MQRankSum=-0.008;QD=14.59;ReadPosRankSum=-1.147;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1119657 . G C 5454.82 . AC=49;AF=0.4375;AN=112;BaseQRankSum=3.181;DP=432;Dels=0.00;FS=3.269;HRun=3;HaplotypeScore=0.4863;InbreedingCoeff=0.1117;MQ=58.71;MQ0=0;MQRankSum=1.349;QD=20.58;ReadPosRankSum=-0.252;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1119796 . C T 91.64 . AC=5;AF=0.078;AN=64;BaseQRankSum=-0.197;DP=63;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.1159;InbreedingCoeff=-0.0863;MQ=58.22;MQ0=0;MQRankSum=0.250;QD=9.16;ReadPosRankSum=-0.408;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1119858 . C T 125.02 . AC=7;AF=0.130;AN=54;BaseQRankSum=-0.015;DP=50;Dels=0.00;FS=4.112;HRun=0;HaplotypeScore=0.0000;InbreedingCoeff=0.0152;MQ=56.27;MQ0=0;MQRankSum=0.412;QD=13.89;ReadPosRankSum=1.205;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12),UPSTREAM(MODIFIER|||||TTLL10-AS1|antisense|NON_CODING|ENST00000379317|);CSQ=upstream_gene_variant|||ENSG00000205231|TTLL10-AS1|ENST00000379317|||||antisense|1,upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1120091 . G A 172.79 . AC=11;AF=0.145;AN=76;BaseQRankSum=0.184;DP=127;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1032;InbreedingCoeff=0.0586;MQ=42.08;MQ0=0;MQRankSum=-1.381;QD=4.80;ReadPosRankSum=1.301;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12);CSQ=upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1120307 . G A 5288.32 . AC=23;AF=0.235;AN=98;BaseQRankSum=-0.512;DP=784;Dels=0.00;FS=3.693;HRun=0;HaplotypeScore=0.5879;InbreedingCoeff=0.2421;MQ=53.35;MQ0=0;MQRankSum=3.646;QD=18.62;ReadPosRankSum=1.917;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12);CSQ=upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1120337 . G A 33.77 . AC=2;AF=0.0167;AN=120;BaseQRankSum=0.146;DP=1640;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.8805;InbreedingCoeff=-0.0159;MQ=54.17;MQ0=0;MQRankSum=2.540;QD=0.70;ReadPosRankSum=2.633;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12);CSQ=upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1120343 . C T 699.62 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.743;DP=1871;Dels=0.00;FS=5.662;HRun=0;HaplotypeScore=1.0425;InbreedingCoeff=-0.0085;MQ=54.11;MQ0=0;MQRankSum=0.033;QD=16.66;ReadPosRankSum=2.495;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|8),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|12),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|12);CSQ=upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,splice_region_variant&intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162571|TTLL10|ENST00000379288||||-/404|protein_coding|1 -chr1 1120370 . C G 708.51 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.070;DP=2729;Dels=0.00;FS=2.564;HRun=0;HaplotypeScore=1.4361;InbreedingCoeff=-0.0084;MQ=54.85;MQ0=0;MQRankSum=2.839;QD=10.12;ReadPosRankSum=1.250;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctg/Gtg|L355V|404|TTLL10|protein_coding|CODING|ENST00000379288|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctg/Gtg|L428V|673|TTLL10|protein_coding|CODING|ENST00000379289|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ctg/Gtg|L428V|673|TTLL10|protein_coding|CODING|ENST00000379290|);CSQ=upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,missense_variant|Ctg/Gtg|L/V|ENSG00000162571|TTLL10|ENST00000379289|13/16|probably_damaging(0.951)|tolerated(0.12)|428/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,missense_variant|Ctg/Gtg|L/V|ENSG00000162571|TTLL10|ENST00000379290|13/16|probably_damaging(0.951)|tolerated(0.12)|428/673|protein_coding|1,missense_variant|Ctg/Gtg|L/V|ENSG00000162571|TTLL10|ENST00000379288|9/9|probably_damaging(0.999)|tolerated(0.12)|355/404|protein_coding|1 -chr1 1120377 . T A 4655.8 . AC=5;AF=0.0417;AN=120;BaseQRankSum=1.090;DP=2884;Dels=0.00;FS=2.193;HRun=1;HaplotypeScore=1.7603;InbreedingCoeff=-0.0435;MQ=55.01;MQ0=0;MQRankSum=6.538;QD=16.22;ReadPosRankSum=-0.513;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aTg/aAg|M357K|404|TTLL10|protein_coding|CODING|ENST00000379288|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aTg/aAg|M430K|673|TTLL10|protein_coding|CODING|ENST00000379289|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aTg/aAg|M430K|673|TTLL10|protein_coding|CODING|ENST00000379290|);CSQ=upstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,missense_variant|aTg/aAg|M/K|ENSG00000162571|TTLL10|ENST00000379289|13/16|benign(0.181)|tolerated(0.99)|430/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,missense_variant|aTg/aAg|M/K|ENSG00000162571|TTLL10|ENST00000379290|13/16|benign(0.181)|tolerated(0.99)|430/673|protein_coding|1,missense_variant|aTg/aAg|M/K|ENSG00000162571|TTLL10|ENST00000379288|9/9|benign(0.088)|tolerated(0.91)|357/404|protein_coding|1 -chr1 1120431 . G A 10163.92 . AC=16;AF=0.1333;AN=120;BaseQRankSum=-1.606;DP=3363;Dels=0.00;FS=20.818;HRun=1;HaplotypeScore=1.5931;InbreedingCoeff=0.1346;MQ=56.00;MQ0=0;MQRankSum=8.100;QD=14.38;ReadPosRankSum=1.101;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGt/aAt|S375N|404|TTLL10|protein_coding|CODING|ENST00000379288|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGt/aAt|S448N|673|TTLL10|protein_coding|CODING|ENST00000379289|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|aGt/aAt|S448N|673|TTLL10|protein_coding|CODING|ENST00000379290|);CSQ=missense_variant&NMD_transcript_variant|aGt/aAt|S/N|ENSG00000162571|TTLL10|ENST00000486379|1/5|unknown(0)||6/45|nonsense_mediated_decay|1,missense_variant|aGt/aAt|S/N|ENSG00000162571|TTLL10|ENST00000379289|13/16|benign(0)|tolerated(1)|448/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,missense_variant|aGt/aAt|S/N|ENSG00000162571|TTLL10|ENST00000379290|13/16|benign(0)|tolerated(1)|448/673|protein_coding|1,missense_variant|aGt/aAt|S/N|ENSG00000162571|TTLL10|ENST00000379288|9/9|benign(0)|tolerated(1)|375/404|protein_coding|1 -chr1 1120536 . G A 3644.72 . AC=9;AF=0.0750;AN=120;BaseQRankSum=-1.719;DP=1842;Dels=0.00;FS=0.462;HRun=0;HaplotypeScore=1.6538;InbreedingCoeff=0.1586;MQ=56.93;MQ0=0;MQRankSum=7.086;QD=15.99;ReadPosRankSum=-0.320;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|13),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|13),UTR_3_PRIME(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|9);CSQ=intron_variant&NMD_transcript_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,3_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|9/9|||-/404|protein_coding|1 -chr1 1120587 . G A 207.5 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.370;DP=1099;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7829;InbreedingCoeff=-0.0101;MQ=57.36;MQ0=0;MQRankSum=0.481;QD=11.53;ReadPosRankSum=0.161;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|13),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|13),UTR_3_PRIME(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|9);CSQ=intron_variant&NMD_transcript_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,3_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|9/9|||-/404|protein_coding|1 -chr1 1120621 . T C 922.19 . AC=6;AF=0.0500;AN=120;BaseQRankSum=-0.439;DP=752;Dels=0.00;FS=2.007;HRun=0;HaplotypeScore=0.6123;InbreedingCoeff=-0.0575;MQ=57.87;MQ0=0;MQRankSum=1.573;QD=10.72;ReadPosRankSum=-1.626;EFF=DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000460998|),DOWNSTREAM(MODIFIER|||||TTLL10|retained_intron|CODING|ENST00000514695|),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379289|13),INTRON(MODIFIER||||673|TTLL10|protein_coding|CODING|ENST00000379290|13),UTR_3_PRIME(MODIFIER||||404|TTLL10|protein_coding|CODING|ENST00000379288|9);CSQ=intron_variant&NMD_transcript_variant|||ENSG00000162571|TTLL10|ENST00000486379||||-/45|nonsense_mediated_decay|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379289||||-/673|protein_coding|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000460998|||||retained_intron|1,downstream_gene_variant|||ENSG00000162571|TTLL10|ENST00000514695|||||retained_intron|1,intron_variant|||ENSG00000162571|TTLL10|ENST00000379290||||-/673|protein_coding|1,3_prime_UTR_variant|||ENSG00000162571|TTLL10|ENST00000379288|9/9|||-/404|protein_coding|1 -chr1 1138735 . C G 141.37 . AC=8;AF=0.118;AN=68;BaseQRankSum=-0.596;DP=90;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3854;InbreedingCoeff=0.1736;MQ=56.04;MQ0=0;MQRankSum=-0.664;QD=11.78;ReadPosRankSum=0.289;EFF=DOWNSTREAM(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|),DOWNSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),DOWNSTREAM(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|),DOWNSTREAM(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|),INTERGENIC(MODIFIER|||||||||);CSQ=downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 -chr1 1138913 . T C 4314.24 . AC=14;AF=0.1167;AN=120;BaseQRankSum=2.277;DP=804;Dels=0.00;FS=7.205;HRun=4;HaplotypeScore=0.3748;InbreedingCoeff=0.2363;MQ=56.65;MQ0=0;MQRankSum=1.461;QD=20.64;ReadPosRankSum=0.717;EFF=DOWNSTREAM(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|),DOWNSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),UTR_3_PRIME(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|5),UTR_3_PRIME(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|4);CSQ=downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,3_prime_UTR_variant|||ENSG00000186891|TNFRSF18|ENST00000379268|5/5|||-/241|protein_coding|1,downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,3_prime_UTR_variant|||ENSG00000186891|TNFRSF18|ENST00000328596|4/4|||-/255|protein_coding|1 -chr1 1138963 . C T 602.78 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.829;DP=1466;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.7567;InbreedingCoeff=-0.0248;MQ=57.17;MQ0=0;MQRankSum=0.908;QD=8.74;ReadPosRankSum=0.934;EFF=DOWNSTREAM(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|),DOWNSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),UTR_3_PRIME(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|5),UTR_3_PRIME(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|4);CSQ=downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,3_prime_UTR_variant|||ENSG00000186891|TNFRSF18|ENST00000379268|5/5|||-/241|protein_coding|1,downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,3_prime_UTR_variant|||ENSG00000186891|TNFRSF18|ENST00000328596|4/4|||-/255|protein_coding|1 -chr1 1139202 . T C 2450.32 . AC=16;AF=0.1333;AN=120;BaseQRankSum=2.131;DP=874;Dels=0.00;FS=3.615;HRun=0;HaplotypeScore=0.9934;InbreedingCoeff=0.3376;MQ=57.90;MQ0=0;MQRankSum=0.238;QD=14.94;ReadPosRankSum=0.958;EFF=DOWNSTREAM(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|),DOWNSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),SYNONYMOUS_CODING(LOW|SILENT|ccA/ccG|P179|255|TNFRSF18|protein_coding|CODING|ENST00000328596|),UTR_3_PRIME(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|5);CSQ=downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,3_prime_UTR_variant|||ENSG00000186891|TNFRSF18|ENST00000379268|5/5|||-/241|protein_coding|1,downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,synonymous_variant|ccA/ccG|P|ENSG00000186891|TNFRSF18|ENST00000328596|4/4|||179/255|protein_coding|1 -chr1 1139216 . G A 192.87 . AC=3;AF=0.0250;AN=120;BaseQRankSum=1.202;DP=865;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6000;InbreedingCoeff=0.3637;MQ=58.10;MQ0=0;MQRankSum=0.774;QD=11.35;ReadPosRankSum=-0.636;EFF=DOWNSTREAM(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|),DOWNSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgt/Tgt|R175C|255|TNFRSF18|protein_coding|CODING|ENST00000328596|),UTR_3_PRIME(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|5);CSQ=downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,3_prime_UTR_variant|||ENSG00000186891|TNFRSF18|ENST00000379268|5/5|||-/241|protein_coding|1,downstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,missense_variant|Cgt/Tgt|R/C|ENSG00000186891|TNFRSF18|ENST00000328596|4/4|benign(0)|tolerated(0.09)|175/255|protein_coding|1 -chr1 1139498 . C T 443.47 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-0.002;DP=812;Dels=0.00;FS=10.375;HRun=0;HaplotypeScore=0.5381;InbreedingCoeff=-0.0387;MQ=58.91;MQ0=0;MQRankSum=0.153;QD=7.92;ReadPosRankSum=-1.689;EFF=INTRON(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|3),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V101M|169|TNFRSF18|protein_coding|CODING|ENST00000486728|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V173M|234|TNFRSF18|protein_coding|CODING|ENST00000379265|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V173M|241|TNFRSF18|protein_coding|CODING|ENST00000379268|);CSQ=missense_variant|Gtg/Atg|V/M|ENSG00000186891|TNFRSF18|ENST00000379265|4/5|benign(0.2)|tolerated(0.25)|173/234|protein_coding|1,missense_variant|Gtg/Atg|V/M|ENSG00000186891|TNFRSF18|ENST00000379268|4/5|benign(0.2)|tolerated(0.26)|173/241|protein_coding|1,missense_variant|Gtg/Atg|V/M|ENSG00000186891|TNFRSF18|ENST00000486728|3/4|benign(0.2)|tolerated(0.14)|101/169|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 -chr1 1139862 . T C 4003.11 . AC=5;AF=0.0417;AN=120;BaseQRankSum=2.163;DP=3444;Dels=0.00;FS=24.530;HRun=0;HaplotypeScore=1.7331;InbreedingCoeff=0.3739;MQ=58.49;MQ0=0;MQRankSum=0.567;QD=18.88;ReadPosRankSum=-5.116;EFF=SYNONYMOUS_CODING(LOW|SILENT|aaA/aaG|K105|234|TNFRSF18|protein_coding|CODING|ENST00000379265|),SYNONYMOUS_CODING(LOW|SILENT|aaA/aaG|K105|241|TNFRSF18|protein_coding|CODING|ENST00000379268|),SYNONYMOUS_CODING(LOW|SILENT|aaA/aaG|K105|255|TNFRSF18|protein_coding|CODING|ENST00000328596|),SYNONYMOUS_CODING(LOW|SILENT|aaA/aaG|K33|169|TNFRSF18|protein_coding|CODING|ENST00000486728|);CSQ=synonymous_variant|aaA/aaG|K|ENSG00000186891|TNFRSF18|ENST00000379265|3/5|||105/234|protein_coding|1,synonymous_variant|aaA/aaG|K|ENSG00000186891|TNFRSF18|ENST00000379268|3/5|||105/241|protein_coding|1,synonymous_variant|aaA/aaG|K|ENSG00000186891|TNFRSF18|ENST00000486728|2/4|||33/169|protein_coding|1,synonymous_variant|aaA/aaG|K|ENSG00000186891|TNFRSF18|ENST00000328596|3/4|||105/255|protein_coding|1 -chr1 1140290 . T C 112.5 . AC=7;AF=0.146;AN=48;BaseQRankSum=1.165;DP=43;Dels=0.00;FS=8.424;HRun=1;HaplotypeScore=0.0347;InbreedingCoeff=0.1666;MQ=58.92;MQ0=0;MQRankSum=-0.621;QD=16.07;ReadPosRankSum=1.709;EFF=INTRON(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|1),INTRON(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|2),INTRON(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|2),INTRON(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|2);CSQ=intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 -chr1 1140336 . C A 41.02 . AC=7;AF=0.175;AN=40;BaseQRankSum=-2.498;DP=35;Dels=0.00;FS=16.166;HRun=3;HaplotypeScore=0.0909;InbreedingCoeff=0.1580;MQ=57.31;MQ0=0;MQRankSum=-0.221;QD=6.84;ReadPosRankSum=-1.676;EFF=INTRON(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|1),INTRON(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|2),INTRON(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|2),INTRON(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|2);CSQ=intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 -chr1 1140435 . G T 467.03 . AC=25;AF=0.463;AN=54;BaseQRankSum=-0.385;DP=39;Dels=0.00;FS=1.380;HRun=1;HaplotypeScore=0.0000;InbreedingCoeff=0.2592;MQ=60.00;MQ0=0;MQRankSum=0.014;QD=24.58;ReadPosRankSum=-1.128;EFF=INTRON(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|1),INTRON(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|2),INTRON(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|2),INTRON(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|2);CSQ=intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 -chr1 1140504 . T C 76.53 . AC=7;AF=0.090;AN=78;BaseQRankSum=-0.513;DP=85;Dels=0.00;FS=7.579;HRun=4;HaplotypeScore=0.1294;InbreedingCoeff=0.1345;MQ=58.35;MQ0=0;MQRankSum=0.232;QD=9.57;ReadPosRankSum=0.795;EFF=INTRON(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|1),INTRON(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|2),INTRON(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|2),INTRON(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|2);CSQ=intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 -chr1 1140812 . TC T 426.77 . AC=1;AF=0.0083;AN=120;BaseQRankSum=3.763;DP=2438;FS=2.359;HRun=1;HaplotypeScore=140.7554;InbreedingCoeff=-0.0094;MQ=58.75;MQ0=0;MQRankSum=1.559;QD=21.34;ReadPosRankSum=0.995;EFF=FRAME_SHIFT(HIGH||-|-11|169|TNFRSF18|protein_coding|CODING|ENST00000486728|1),FRAME_SHIFT(HIGH||-|-83|234|TNFRSF18|protein_coding|CODING|ENST00000379265|2),FRAME_SHIFT(HIGH||-|-83|241|TNFRSF18|protein_coding|CODING|ENST00000379268|2),FRAME_SHIFT(HIGH||-|-83|255|TNFRSF18|protein_coding|CODING|ENST00000328596|2);CSQ=frameshift_variant&feature_truncation|||ENSG00000186891|TNFRSF18|ENST00000379265|2/5|||83/234|protein_coding|1,frameshift_variant&feature_truncation|||ENSG00000186891|TNFRSF18|ENST00000379268|2/5|||83/241|protein_coding|1,frameshift_variant&feature_truncation|||ENSG00000186891|TNFRSF18|ENST00000486728|1/4|||11/169|protein_coding|1,frameshift_variant&feature_truncation|||ENSG00000186891|TNFRSF18|ENST00000328596|2/4|||83/255|protein_coding|1 -chr1 1141556 . G C 130.07 . AC=7;AF=0.097;AN=72;BaseQRankSum=0.191;DP=66;Dels=0.00;FS=4.782;HRun=3;HaplotypeScore=0.1198;InbreedingCoeff=-0.0389;MQ=57.15;MQ0=0;MQRankSum=-0.406;QD=11.82;ReadPosRankSum=-0.358;EFF=INTRON(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|1),INTRON(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|1),INTRON(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|1),UPSTREAM(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|);CSQ=intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 -chr1 1141723 . A G 877.55 . AC=13;AF=0.1204;AN=108;BaseQRankSum=0.547;DP=397;Dels=0.00;FS=2.262;HRun=3;HaplotypeScore=0.1820;InbreedingCoeff=0.0234;MQ=58.98;MQ0=0;MQRankSum=0.061;QD=11.40;ReadPosRankSum=-1.301;EFF=DOWNSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),DOWNSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),INTRON(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|1),INTRON(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|1),INTRON(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|1),UPSTREAM(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|);CSQ=intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,intron_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 -chr1 1142399 . A AC 117.53 . AC=8;AF=0.308;AN=26;BaseQRankSum=-2.601;DP=22;FS=0.000;HRun=4;HaplotypeScore=4.5003;InbreedingCoeff=0.4087;MQ=58.95;MQ0=0;MQRankSum=0.702;QD=23.51;ReadPosRankSum=0.537;EFF=DOWNSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),DOWNSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||169|TNFRSF18|protein_coding|CODING|ENST00000486728|),UPSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),UPSTREAM(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|),UPSTREAM(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|);CSQ=downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000486728||||-/169|protein_coding|1,downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 -chr1 1146478 . T C 51.13 . AC=2;AF=0.050;AN=40;BaseQRankSum=-1.556;DP=39;Dels=0.00;FS=14.569;HRun=2;HaplotypeScore=0.1961;InbreedingCoeff=-0.2329;MQ=56.11;MQ0=0;MQRankSum=-1.373;QD=5.11;ReadPosRankSum=-1.373;EFF=DOWNSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),DOWNSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),INTERGENIC(MODIFIER|||||||||),UPSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),UPSTREAM(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|),UPSTREAM(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|);CSQ=downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 -chr1 1146785 . G C 472.67 . AC=4;AF=0.0333;AN=120;BaseQRankSum=0.520;DP=773;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3659;InbreedingCoeff=-0.0419;MQ=55.65;MQ0=0;MQRankSum=2.347;QD=10.28;ReadPosRankSum=0.755;EFF=DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),EXON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|5),UPSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),UPSTREAM(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|),UPSTREAM(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|),UTR_3_PRIME(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|7);CSQ=downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|5/5||||retained_intron|1,3_prime_UTR_variant|||ENSG00000186827|TNFRSF4|ENST00000379236|7/7|||-/277|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 -chr1 1146853 . G A 316.99 . AC=4;AF=0.0339;AN=118;BaseQRankSum=-0.212;DP=1329;Dels=0.00;FS=8.508;HRun=0;HaplotypeScore=1.8407;InbreedingCoeff=-0.0495;MQ=50.41;MQ0=0;MQRankSum=0.063;QD=4.12;ReadPosRankSum=-1.639;EFF=DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),EXON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|5),UPSTREAM(MODIFIER||||234|TNFRSF18|protein_coding|CODING|ENST00000379265|),UPSTREAM(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|),UPSTREAM(MODIFIER||||255|TNFRSF18|protein_coding|CODING|ENST00000328596|),UTR_3_PRIME(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|7);CSQ=downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379265||||-/234|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|5/5||||retained_intron|1,3_prime_UTR_variant|||ENSG00000186827|TNFRSF4|ENST00000379236|7/7|||-/277|protein_coding|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000328596||||-/255|protein_coding|1 -chr1 1146965 . G A 553.08 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.055;DP=3459;Dels=0.00;FS=2.776;HRun=0;HaplotypeScore=2.1270;InbreedingCoeff=-0.0086;MQ=56.15;MQ0=0;MQRankSum=0.024;QD=9.22;ReadPosRankSum=0.233;EFF=DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),EXON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|5),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A268|277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|);CSQ=downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|5/5||||retained_intron|1,synonymous_variant|gcC/gcT|A|ENSG00000186827|TNFRSF4|ENST00000379236|7/7|||268/277|protein_coding|1 -chr1 1147024 . G A 429.09 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.041;DP=2325;Dels=0.00;FS=8.212;HRun=1;HaplotypeScore=2.0094;InbreedingCoeff=-0.0086;MQ=56.92;MQ0=0;MQRankSum=-0.049;QD=10.73;ReadPosRankSum=0.293;EFF=DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),EXON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|5),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|6),UPSTREAM(MODIFIER||||241|TNFRSF18|protein_coding|CODING|ENST00000379268|);CSQ=downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,upstream_gene_variant|||ENSG00000186891|TNFRSF18|ENST00000379268||||-/241|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|5/5||||retained_intron|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1 -chr1 1147243 . A C 738.87 . AC=6;AF=0.0508;AN=118;BaseQRankSum=-0.595;DP=705;Dels=0.00;FS=3.973;HRun=2;HaplotypeScore=0.6191;InbreedingCoeff=-0.0615;MQ=56.78;MQ0=0;MQRankSum=-0.060;QD=13.94;ReadPosRankSum=-0.426;EFF=DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|5),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|4);CSQ=downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1 -chr1 1147297 . G A 1091.92 . AC=6;AF=0.0508;AN=118;BaseQRankSum=-0.085;DP=1113;Dels=0.00;FS=5.569;HRun=0;HaplotypeScore=0.7306;InbreedingCoeff=-0.0597;MQ=55.37;MQ0=0;MQRankSum=0.517;QD=12.55;ReadPosRankSum=1.988;EFF=DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|5),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|4);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1 -chr1 1147337 . C T 274.47 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.893;DP=1689;Dels=0.00;FS=5.279;HRun=0;HaplotypeScore=1.6439;InbreedingCoeff=-0.0134;MQ=55.97;MQ0=0;MQRankSum=3.240;QD=11.93;ReadPosRankSum=0.328;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),DOWNSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),EXON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|4),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V207M|277|TNFRSF4|protein_coding|CODING|ENST00000379236|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,missense_variant|Gtg/Atg|V/M|ENSG00000186827|TNFRSF4|ENST00000379236|5/7|benign(0.024)|tolerated(0.21)|207/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|4/5||||retained_intron|1 -chr1 1147422 . C T 34265.37 . AC=47;AF=0.3917;AN=120;BaseQRankSum=-3.610;DP=3511;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=2.1684;InbreedingCoeff=0.1904;MQ=57.58;MQ0=0;MQRankSum=0.337;QD=16.76;ReadPosRankSum=-0.698;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),EXON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|4),EXON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|4),SYNONYMOUS_CODING(LOW|SILENT|gaG/gaA|E178|277|TNFRSF4|protein_coding|CODING|ENST00000379236|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|4/4||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,synonymous_variant|gaG/gaA|E|ENSG00000186827|TNFRSF4|ENST00000379236|5/7|||178/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|4/5||||retained_intron|1 -chr1 1147655 . G A 774.9 . AC=2;AF=0.0172;AN=116;BaseQRankSum=-2.234;DP=863;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5174;InbreedingCoeff=-0.0388;MQ=57.75;MQ0=0;MQRankSum=2.030;QD=11.07;ReadPosRankSum=-0.644;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|4),INTRON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|3),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|3);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 -chr1 1147770 . G A 69.21 . AC=2;AF=0.0196;AN=102;BaseQRankSum=-0.351;DP=166;Dels=0.00;FS=2.592;HRun=0;HaplotypeScore=0.1050;InbreedingCoeff=-0.1133;MQ=58.60;MQ0=0;MQRankSum=0.830;QD=7.69;ReadPosRankSum=-1.788;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|4),INTRON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|3),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|3);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 -chr1 1147822 . C T 59.82 . AC=2;AF=0.023;AN=88;BaseQRankSum=-1.601;DP=102;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.0561;InbreedingCoeff=0.0325;MQ=56.99;MQ0=0;MQRankSum=1.803;QD=11.96;ReadPosRankSum=-2.039;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|4),INTRON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|3),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|3);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 -chr1 1147976 . A AC 257.05 . AC=13;AF=0.1182;AN=110;BaseQRankSum=3.114;DP=421;FS=9.449;HRun=9;HaplotypeScore=46.1189;InbreedingCoeff=0.0619;MQ=53.84;MQ0=0;MQRankSum=-4.864;QD=2.79;ReadPosRankSum=-0.046;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|4),INTRON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|3),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|3);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 -chr1 1147983 . C T 450.24 . AC=6;AF=0.0545;AN=110;BaseQRankSum=-1.143;DP=492;Dels=0.01;FS=1.490;HRun=0;HaplotypeScore=2.2660;InbreedingCoeff=-0.0917;MQ=53.74;MQ0=0;MQRankSum=-5.572;QD=5.85;ReadPosRankSum=-5.673;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|4),INTRON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|3),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|3);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 -chr1 1147985 . C A 474.8 . AC=7;AF=0.0648;AN=108;BaseQRankSum=1.860;DP=505;Dels=0.01;FS=0.000;HRun=1;HaplotypeScore=2.2858;InbreedingCoeff=-0.0990;MQ=53.76;MQ0=0;MQRankSum=-5.601;QD=5.59;ReadPosRankSum=-5.790;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|4),INTRON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|3),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|3);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 -chr1 1148100 . G A 407.86 . AC=4;AF=0.0333;AN=120;BaseQRankSum=0.544;DP=1027;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7645;InbreedingCoeff=-0.0459;MQ=57.31;MQ0=0;MQRankSum=2.510;QD=7.16;ReadPosRankSum=-1.015;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|3),INTRON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|2),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|2);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 -chr1 1148354 . C G 126.37 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.471;DP=949;Dels=0.00;FS=7.261;HRun=1;HaplotypeScore=0.7234;InbreedingCoeff=-0.0178;MQ=58.63;MQ0=0;MQRankSum=0.297;QD=7.43;ReadPosRankSum=0.432;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|3),INTRON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|2),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|2);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 -chr1 1148445 . G A 646.52 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-2.708;DP=1141;Dels=0.00;FS=9.672;HRun=0;HaplotypeScore=0.7418;InbreedingCoeff=-0.0474;MQ=58.91;MQ0=0;MQRankSum=1.939;QD=10.60;ReadPosRankSum=0.841;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),EXON(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|2),EXON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|2),SYNONYMOUS_CODING(LOW|SILENT|tgC/tgT|C99|277|TNFRSF4|protein_coding|CODING|ENST00000379236|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|2/4||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,synonymous_variant|tgC/tgT|C|ENSG00000186827|TNFRSF4|ENST00000379236|3/7|||99/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|2/5||||retained_intron|1 -chr1 1149222 . C G 291.64 . AC=2;AF=0.0169;AN=118;BaseQRankSum=1.786;DP=868;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7514;InbreedingCoeff=-0.0246;MQ=55.49;MQ0=0;MQRankSum=1.770;QD=10.80;ReadPosRankSum=1.215;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|1),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|1),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 -chr1 1149272 . T C 57.68 . AC=9;AF=0.0750;AN=120;BaseQRankSum=-9.463;DP=854;Dels=0.00;FS=62.532;HRun=5;HaplotypeScore=2.2956;InbreedingCoeff=-0.0979;MQ=53.68;MQ0=0;MQRankSum=-8.286;QD=0.57;ReadPosRankSum=-1.613;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|1),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|1),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 -chr1 1149316 . C G 1809.62 . AC=6;AF=0.0500;AN=120;BaseQRankSum=-2.427;DP=1206;Dels=0.00;FS=7.512;HRun=0;HaplotypeScore=1.0567;InbreedingCoeff=0.2590;MQ=55.74;MQ0=0;MQRankSum=3.245;QD=14.14;ReadPosRankSum=1.300;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),INTRON(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|1),INTRON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|1),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1 -chr1 1149480 . G A 811.03 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.272;DP=1806;Dels=0.00;FS=0.697;HRun=0;HaplotypeScore=1.2218;InbreedingCoeff=-0.0227;MQ=56.24;MQ0=0;MQRankSum=1.175;QD=12.48;ReadPosRankSum=1.574;EFF=DOWNSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),DOWNSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),DOWNSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),DOWNSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|),EXON(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgc/Tgc|R10C|277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,missense_variant|Cgc/Tgc|R/C|ENSG00000186827|TNFRSF4|ENST00000379236|1/7|unknown(0)|deleterious(0)|10/277|protein_coding|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|1/5||||retained_intron|1 -chr1 1152631 . C A 73.67 . AC=6;AF=0.333;AN=18;BaseQRankSum=1.036;DP=11;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0000;MQ=57.87;MQ0=0;MQRankSum=0.000;QD=18.42;ReadPosRankSum=0.365;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|2),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|3),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),UTR_3_PRIME(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|7),UTR_3_PRIME(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|7),UTR_3_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|7);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727|7/7|||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|3/3||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|2/2||||retained_intron|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000263741|7/7|||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000360001|7/7|||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1152794 . A G 38.74 . AC=6;AF=0.250;AN=24;BaseQRankSum=0.720;DP=37;Dels=0.00;FS=0.000;HRun=3;HaplotypeScore=0.4589;InbreedingCoeff=0.4010;MQ=52.01;MQ0=0;MQRankSum=-2.348;QD=12.91;ReadPosRankSum=-0.407;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|2),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|3),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),UTR_3_PRIME(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|7),UTR_3_PRIME(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|7),UTR_3_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|7);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727|7/7|||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|3/3||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|2/2||||retained_intron|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000263741|7/7|||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000360001|7/7|||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1152798 . A C 38.42 . AC=6;AF=0.250;AN=24;BaseQRankSum=0.147;DP=40;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.4236;InbreedingCoeff=0.4263;MQ=52.65;MQ0=0;MQRankSum=-2.740;QD=12.81;ReadPosRankSum=-1.267;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|2),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|3),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),UTR_3_PRIME(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|7),UTR_3_PRIME(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|7),UTR_3_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|7);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727|7/7|||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|3/3||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|2/2||||retained_intron|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000263741|7/7|||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000360001|7/7|||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1152800 . G C 38.25 . AC=6;AF=0.250;AN=24;BaseQRankSum=-0.172;DP=40;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1945;InbreedingCoeff=0.4359;MQ=52.65;MQ0=0;MQRankSum=-2.690;QD=12.75;ReadPosRankSum=-2.003;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|2),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|3),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),UTR_3_PRIME(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|7),UTR_3_PRIME(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|7),UTR_3_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|7);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727|7/7|||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|3/3||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|2/2||||retained_intron|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000263741|7/7|||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000360001|7/7|||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1152904 . G A 446.25 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.116;DP=2117;Dels=0.00;FS=5.998;HRun=0;HaplotypeScore=1.1651;InbreedingCoeff=-0.0085;MQ=59.10;MQ0=0;MQRankSum=0.448;QD=16.53;ReadPosRankSum=0.722;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|2),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|3),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H359|362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),UTR_3_PRIME(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|7),UTR_3_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|7);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727|7/7|||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|3/3||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|2/2||||retained_intron|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000263741|7/7|||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,synonymous_variant|caC/caT|H|ENSG00000078808|SDF4|ENST00000360001|7/7|||359/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1153009 . G A 2720.69 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-1.647;DP=5548;Dels=0.00;FS=10.837;HRun=0;HaplotypeScore=3.0829;InbreedingCoeff=0.9964;MQ=59.14;MQ0=0;MQRankSum=-1.581;QD=29.57;ReadPosRankSum=-0.625;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|2),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|3),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A324|362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),UTR_3_PRIME(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|7),UTR_3_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|7);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727|7/7|||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|3/3||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|2/2||||retained_intron|1,3_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000263741|7/7|||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,synonymous_variant|gcC/gcT|A|ENSG00000078808|SDF4|ENST00000360001|7/7|||324/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1153113 . G A 16021.84 . AC=14;AF=0.1167;AN=120;BaseQRankSum=1.612;DP=4750;Dels=0.00;FS=29.208;HRun=1;HaplotypeScore=3.8427;InbreedingCoeff=0.1934;MQ=58.59;MQ0=0;MQRankSum=-0.299;QD=16.67;ReadPosRankSum=2.245;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|6),INTRON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|1),INTRON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|2),SYNONYMOUS_CODING(LOW|SILENT|ctC/ctT|L328|348|SDF4|protein_coding|CODING|ENST00000263741|),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|),UTR_3_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|7);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727|7/7|||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,synonymous_variant|ctC/ctT|L|ENSG00000078808|SDF4|ENST00000263741|7/7|||328/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1153658 . C T 180.9 . AC=3;AF=0.0254;AN=118;BaseQRankSum=-1.007;DP=436;Dels=0.00;FS=8.257;HRun=0;HaplotypeScore=0.1613;InbreedingCoeff=-0.0432;MQ=57.74;MQ0=0;MQRankSum=-0.296;QD=10.05;ReadPosRankSum=0.424;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|6),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|6),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|6),INTRON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|1),INTRON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|2),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1153671 . C T 201.6 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.401;DP=557;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3657;InbreedingCoeff=-0.0187;MQ=57.34;MQ0=0;MQRankSum=0.747;QD=20.16;ReadPosRankSum=1.045;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|6),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|6),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|6),INTRON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|1),INTRON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|2),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|processed_transcript|CODING|ENST00000453580|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|);CSQ=downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000453580|||||processed_transcript|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1154121 . G A 626.94 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.070;DP=2668;Dels=0.00;FS=1.726;HRun=0;HaplotypeScore=1.8437;InbreedingCoeff=-0.0088;MQ=57.56;MQ0=0;MQRankSum=2.723;QD=11.61;ReadPosRankSum=-0.999;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|1),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|5),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|5),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|5),INTRON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|1),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|1/2||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1 -chr1 1154374 . G A 229.71 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.034;DP=1145;Dels=0.00;FS=3.775;HRun=1;HaplotypeScore=0.6454;InbreedingCoeff=-0.0116;MQ=58.50;MQ0=0;MQRankSum=0.802;QD=7.92;ReadPosRankSum=0.928;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|1),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|1),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER||||277|TNFRSF4|protein_coding|CODING|ENST00000379236|),UPSTREAM(MODIFIER|||||TNFRSF4|retained_intron|CODING|ENST00000497869|);CSQ=missense_variant|Cgg/Tgg|R/W|ENSG00000078808|SDF4|ENST00000403997|4/5|possibly_damaging(0.833)|deleterious(0.02)|132/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|1/3||||retained_intron|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000497869|||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|1/2||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000186827|TNFRSF4|ENST00000379236||||-/277|protein_coding|1 -chr1 1154539 . G A 108.45 . AC=6;AF=0.068;AN=88;BaseQRankSum=-1.095;DP=122;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0697;InbreedingCoeff=0.0875;MQ=56.71;MQ0=0;MQRankSum=-1.837;QD=10.84;ReadPosRankSum=1.197;EFF=DOWNSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|1),EXON(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|1),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000494748|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000478938|1/2||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1 -chr1 1158070 . C T 64.6 . AC=4;AF=0.154;AN=26;BaseQRankSum=-0.623;DP=14;Dels=0.00;FS=2.688;HRun=0;HaplotypeScore=0.1231;InbreedingCoeff=0.1888;MQ=58.33;MQ0=0;MQRankSum=-1.090;QD=21.53;ReadPosRankSum=-1.713;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1158277 . G A 258.06 . AC=7;AF=0.0660;AN=106;BaseQRankSum=1.218;DP=155;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0943;InbreedingCoeff=0.0730;MQ=58.19;MQ0=0;MQRankSum=0.130;QD=14.34;ReadPosRankSum=0.779;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1158326 . G A 805.01 . AC=13;AF=0.1102;AN=118;BaseQRankSum=1.271;DP=279;Dels=0.00;FS=5.053;HRun=0;HaplotypeScore=0.2930;InbreedingCoeff=0.1676;MQ=57.56;MQ0=0;MQRankSum=-0.687;QD=17.13;ReadPosRankSum=-0.913;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1158357 . A G 916.43 . AC=10;AF=0.0833;AN=120;BaseQRankSum=-1.540;DP=426;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.2650;InbreedingCoeff=0.0655;MQ=57.91;MQ0=0;MQRankSum=-0.479;QD=14.10;ReadPosRankSum=1.027;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1158427 . C CTCAT 100.01 . AC=7;AF=0.0583;AN=120;BaseQRankSum=4.538;DP=1071;FS=0.000;HRun=0;HaplotypeScore=95.9447;InbreedingCoeff=-0.0617;MQ=58.35;MQ0=0;MQRankSum=-4.421;QD=0.89;ReadPosRankSum=1.472;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1158441 . GCA G 2419.37 . AC=7;AF=0.0583;AN=120;BaseQRankSum=8.159;DP=1188;FS=0.719;HRun=0;HaplotypeScore=80.9615;InbreedingCoeff=-0.0625;MQ=58.49;MQ0=0;MQRankSum=0.055;QD=13.82;ReadPosRankSum=-1.278;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1158534 . G GAC 10837.11 . AC=11;AF=0.0917;AN=120;BaseQRankSum=10.503;DP=3575;FS=3.271;HRun=0;HaplotypeScore=253.2703;InbreedingCoeff=0.0992;MQ=58.17;MQ0=0;MQRankSum=-7.407;QD=20.14;ReadPosRankSum=-0.456;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1158543 . G A 443.06 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.994;DP=3849;Dels=0.00;FS=6.053;HRun=0;HaplotypeScore=6.1083;InbreedingCoeff=-0.0084;MQ=58.27;MQ0=0;MQRankSum=0.950;QD=14.77;ReadPosRankSum=-2.545;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1158562 . AAC A 12359.87 . AC=11;AF=0.0917;AN=120;BaseQRankSum=19.423;DP=4760;FS=4.102;HRun=0;HaplotypeScore=407.7083;InbreedingCoeff=0.0992;MQ=58.43;MQ0=0;MQRankSum=-2.532;QD=17.12;ReadPosRankSum=-0.503;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1158567 . A G 234.79 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-2.278;DP=5021;Dels=0.00;FS=38.170;HRun=0;HaplotypeScore=12.5431;InbreedingCoeff=-0.0170;MQ=58.49;MQ0=0;MQRankSum=-1.361;QD=1.06;ReadPosRankSum=-7.229;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|4),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|4),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|4),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1158631 . A G 241536.85 . AC=114;AF=0.9500;AN=120;BaseQRankSum=1.928;DP=8050;DS;Dels=0.00;FS=8.602;HRun=0;HaplotypeScore=3.7991;InbreedingCoeff=-0.0526;MQ=58.98;MQ0=0;MQRankSum=-0.435;QD=30.00;ReadPosRankSum=-0.542;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),EXON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),SYNONYMOUS_CODING(LOW|SILENT|gaT/gaC|D190|202|SDF4|protein_coding|CODING|ENST00000545427|),SYNONYMOUS_CODING(LOW|SILENT|gaT/gaC|D190|348|SDF4|protein_coding|CODING|ENST00000263741|),SYNONYMOUS_CODING(LOW|SILENT|gaT/gaC|D190|362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=synonymous_variant|gaT/gaC|D|ENSG00000078808|SDF4|ENST00000403997|3/5|||125/243|protein_coding|1,synonymous_variant&NMD_transcript_variant|gaT/gaC|D|ENSG00000078808|SDF4|ENST00000465727|4/7|||190/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,synonymous_variant|gaT/gaC|D|ENSG00000078808|SDF4|ENST00000263741|4/7|||190/348|protein_coding|1,synonymous_variant|gaT/gaC|D|ENSG00000078808|SDF4|ENST00000360001|4/7|||190/362|protein_coding|1,synonymous_variant|gaT/gaC|D|ENSG00000078808|SDF4|ENST00000545427|4/5|||190/202|protein_coding|1 -chr1 1158667 . G A 386.78 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.651;DP=8431;DS;Dels=0.00;FS=0.701;HRun=0;HaplotypeScore=4.5114;InbreedingCoeff=-0.0084;MQ=59.13;MQ0=0;MQRankSum=2.552;QD=11.72;ReadPosRankSum=-0.218;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),EXON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|4),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A178|202|SDF4|protein_coding|CODING|ENST00000545427|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A178|348|SDF4|protein_coding|CODING|ENST00000263741|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A178|362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=synonymous_variant|gcC/gcT|A|ENSG00000078808|SDF4|ENST00000403997|3/5|||113/243|protein_coding|1,synonymous_variant&NMD_transcript_variant|gcC/gcT|A|ENSG00000078808|SDF4|ENST00000465727|4/7|||178/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,synonymous_variant|gcC/gcT|A|ENSG00000078808|SDF4|ENST00000263741|4/7|||178/348|protein_coding|1,synonymous_variant|gcC/gcT|A|ENSG00000078808|SDF4|ENST00000360001|4/7|||178/362|protein_coding|1,synonymous_variant|gcC/gcT|A|ENSG00000078808|SDF4|ENST00000545427|4/5|||178/202|protein_coding|1 -chr1 1158948 . A G 660.52 . AC=12;AF=0.1034;AN=116;BaseQRankSum=1.771;DP=442;Dels=0.00;FS=0.846;HRun=1;HaplotypeScore=0.2202;InbreedingCoeff=0.0086;MQ=59.04;MQ0=0;MQRankSum=1.540;QD=9.57;ReadPosRankSum=1.061;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1158973 . A T 563.68 . AC=11;AF=0.0932;AN=118;BaseQRankSum=-1.928;DP=394;Dels=0.00;FS=3.023;HRun=0;HaplotypeScore=0.2474;InbreedingCoeff=0.1727;MQ=58.52;MQ0=0;MQRankSum=0.128;QD=11.74;ReadPosRankSum=-0.053;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1158975 . A C 547.46 . AC=10;AF=0.0847;AN=118;BaseQRankSum=0.388;DP=392;Dels=0.00;FS=6.029;HRun=1;HaplotypeScore=0.2379;InbreedingCoeff=0.1826;MQ=58.58;MQ0=0;MQRankSum=-0.688;QD=12.73;ReadPosRankSum=0.857;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159020 . A G 979.85 . AC=12;AF=0.1000;AN=120;BaseQRankSum=0.104;DP=496;Dels=0.00;FS=2.803;HRun=0;HaplotypeScore=0.5310;InbreedingCoeff=0.1557;MQ=58.21;MQ0=0;MQRankSum=1.781;QD=13.80;ReadPosRankSum=-2.245;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159024 . C T 1054.21 . AC=12;AF=0.1000;AN=120;BaseQRankSum=1.335;DP=502;Dels=0.00;FS=1.571;HRun=1;HaplotypeScore=0.4565;InbreedingCoeff=0.1688;MQ=58.19;MQ0=0;MQRankSum=-0.153;QD=14.25;ReadPosRankSum=-1.099;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159062 . T C 2118.4 . AC=9;AF=0.0833;AN=108;BaseQRankSum=4.132;DP=759;Dels=0.00;FS=2.568;HRun=0;HaplotypeScore=0.5732;InbreedingCoeff=0.1030;MQ=58.19;MQ0=0;MQRankSum=0.274;QD=18.11;ReadPosRankSum=0.765;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159107 . G A 3662.49 . AC=10;AF=0.0926;AN=108;BaseQRankSum=1.981;DP=1308;Dels=0.00;FS=17.272;HRun=0;HaplotypeScore=1.1121;InbreedingCoeff=0.0871;MQ=52.60;MQ0=0;MQRankSum=2.390;QD=16.35;ReadPosRankSum=1.115;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159165 . T TG 273.77 . AC=12;AF=0.1132;AN=106;BaseQRankSum=7.494;DP=2291;FS=100.981;HRun=1;HaplotypeScore=440.9019;InbreedingCoeff=-0.1415;MQ=41.07;MQ0=0;MQRankSum=-7.908;QD=0.51;ReadPosRankSum=7.023;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159166 . G GC 3218.59 . AC=42;AF=0.3962;AN=106;BaseQRankSum=8.299;DP=2329;FS=244.281;HRun=3;HaplotypeScore=445.7226;InbreedingCoeff=-0.6799;MQ=40.93;MQ0=0;MQRankSum=-10.648;QD=1.55;ReadPosRankSum=6.277;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159170 . G C 5417.07 . AC=46;AF=0.4182;AN=110;BaseQRankSum=-10.941;DP=2442;Dels=0.01;FS=490.296;HRun=4;HaplotypeScore=12.6085;InbreedingCoeff=-0.7658;MQ=41.38;MQ0=0;MQRankSum=-18.721;QD=2.30;ReadPosRankSum=6.341;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159170 . G GC 3955.45 . AC=43;AF=0.3909;AN=110;BaseQRankSum=10.923;DP=2473;FS=560.241;HRun=4;HaplotypeScore=452.2142;InbreedingCoeff=-0.6871;MQ=41.56;MQ0=0;MQRankSum=-13.253;QD=1.73;ReadPosRankSum=9.759;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159174 . CG C 53.97 . AC=7;AF=0.0614;AN=114;BaseQRankSum=10.925;DP=2603;FS=201.126;HRun=1;HaplotypeScore=455.1303;InbreedingCoeff=-0.0824;MQ=42.56;MQ0=0;MQRankSum=-11.905;QD=0.19;ReadPosRankSum=3.289;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159175 . G C 12381.61 . AC=47;AF=0.4123;AN=114;BaseQRankSum=-13.988;DP=2603;Dels=0.01;FS=959.340;HRun=4;HaplotypeScore=13.4110;InbreedingCoeff=-0.7559;MQ=42.56;MQ0=0;MQRankSum=-22.983;QD=4.80;ReadPosRankSum=11.432;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159175 . G GC 1388.08 . AC=33;AF=0.2895;AN=114;BaseQRankSum=15.983;DP=2657;FS=479.390;HRun=4;HaplotypeScore=447.3152;InbreedingCoeff=-0.4239;MQ=42.88;MQ0=0;MQRankSum=-14.282;QD=0.74;ReadPosRankSum=11.216;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159179 . A C 7593.73 . AC=46;AF=0.3898;AN=118;BaseQRankSum=-14.894;DP=2793;Dels=0.01;FS=168.118;HRun=3;HaplotypeScore=10.8241;InbreedingCoeff=-0.6865;MQ=44.03;MQ0=0;MQRankSum=-23.118;QD=2.84;ReadPosRankSum=9.565;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159179 . A AC 430.63 . AC=15;AF=0.1293;AN=116;BaseQRankSum=17.141;DP=2801;FS=268.128;HRun=3;HaplotypeScore=446.6273;InbreedingCoeff=-0.1719;MQ=43.85;MQ0=0;MQRankSum=-15.854;QD=0.50;ReadPosRankSum=8.601;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159182 . G C 2435.95 . AC=35;AF=0.3017;AN=116;BaseQRankSum=-22.569;DP=2867;Dels=0.01;FS=332.999;HRun=4;HaplotypeScore=9.7305;InbreedingCoeff=-0.4479;MQ=44.51;MQ0=0;MQRankSum=-21.997;QD=1.18;ReadPosRankSum=7.752;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159182 . G GC 290.6 . AC=11;AF=0.0948;AN=116;BaseQRankSum=18.934;DP=2960;FS=357.719;HRun=4;HaplotypeScore=463.0417;InbreedingCoeff=-0.1293;MQ=44.74;MQ0=0;MQRankSum=-17.632;QD=0.45;ReadPosRankSum=5.626;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159186 . CA C 59.87 . AC=4;AF=0.0345;AN=116;BaseQRankSum=16.532;DP=3075;FS=138.229;HRun=1;HaplotypeScore=462.1606;InbreedingCoeff=-0.0421;MQ=45.70;MQ0=0;MQRankSum=-13.262;QD=0.31;ReadPosRankSum=-6.897;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_truncation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159187 . A C 8669.24 . AC=47;AF=0.4052;AN=116;BaseQRankSum=-11.349;DP=3075;Dels=0.01;FS=50.216;HRun=4;HaplotypeScore=10.3138;InbreedingCoeff=-0.7071;MQ=45.70;MQ0=0;MQRankSum=-25.197;QD=2.88;ReadPosRankSum=-1.026;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159187 . A AC 38.33 . AC=3;AF=0.0259;AN=116;BaseQRankSum=18.228;DP=3113;FS=209.724;HRun=4;HaplotypeScore=455.4440;InbreedingCoeff=-0.0431;MQ=45.73;MQ0=0;MQRankSum=-16.454;QD=0.25;ReadPosRankSum=-6.893;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159191 . A C 4227.25 . AC=45;AF=0.3814;AN=118;BaseQRankSum=-16.791;DP=3098;Dels=0.00;FS=55.651;HRun=4;HaplotypeScore=21.4245;InbreedingCoeff=-0.6257;MQ=46.09;MQ0=0;MQRankSum=-20.550;QD=1.47;ReadPosRankSum=-6.333;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|3),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|3),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|3),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|3),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159485 . C T 1786.91 . AC=11;AF=0.0917;AN=120;BaseQRankSum=-1.460;DP=676;Dels=0.00;FS=1.200;HRun=0;HaplotypeScore=0.4563;InbreedingCoeff=0.0725;MQ=57.99;MQ0=0;MQRankSum=0.146;QD=16.39;ReadPosRankSum=0.465;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159517 . T C 12932.29 . AC=115;AF=0.9583;AN=120;BaseQRankSum=1.742;DP=373;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.2819;InbreedingCoeff=0.1828;MQ=58.44;MQ0=0;MQRankSum=1.074;QD=35.14;ReadPosRankSum=1.392;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159520 . C T 92.03 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.994;DP=360;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.2826;InbreedingCoeff=-0.0403;MQ=58.45;MQ0=0;MQRankSum=0.711;QD=13.15;ReadPosRankSum=0.622;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159540 . G A 542.38 . AC=6;AF=0.0517;AN=116;BaseQRankSum=-0.694;DP=294;Dels=0.00;FS=4.088;HRun=0;HaplotypeScore=0.1520;InbreedingCoeff=0.0954;MQ=58.66;MQ0=0;MQRankSum=1.126;QD=17.50;ReadPosRankSum=0.267;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1159729 . T G 41.48 . AC=5;AF=0.119;AN=42;BaseQRankSum=0.407;DP=35;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0000;InbreedingCoeff=0.0685;MQ=55.69;MQ0=0;MQRankSum=-0.031;QD=10.37;ReadPosRankSum=0.470;EFF=DOWNSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000478938|),UPSTREAM(MODIFIER|||||SDF4|retained_intron|CODING|ENST00000494748|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000494748|||||retained_intron|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000478938|||||retained_intron|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1 -chr1 1163767 . C T 814.94 . AC=5;AF=0.0446;AN=112;BaseQRankSum=-1.291;DP=646;Dels=0.00;FS=1.287;HRun=0;HaplotypeScore=0.6392;InbreedingCoeff=0.1958;MQ=57.92;MQ0=0;MQRankSum=-5.203;QD=14.05;ReadPosRankSum=2.369;EFF=INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),INTRON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 -chr1 1163773 . C T 190.62 . AC=2;AF=0.0182;AN=110;BaseQRankSum=-0.471;DP=694;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6224;InbreedingCoeff=0.4388;MQ=58.00;MQ0=0;MQRankSum=0.927;QD=23.83;ReadPosRankSum=2.443;EFF=INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),INTRON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 -chr1 1163804 . C T 3932.35 . AC=16;AF=0.1356;AN=118;BaseQRankSum=-5.937;DP=1136;Dels=0.00;FS=0.471;HRun=0;HaplotypeScore=1.9937;InbreedingCoeff=-0.0388;MQ=57.51;MQ0=0;MQRankSum=2.984;QD=13.15;ReadPosRankSum=-2.512;EFF=INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),INTRON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=intron_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 -chr1 1163811 . G GGACA 6953.39 . AC=15;AF=0.1271;AN=118;BaseQRankSum=-5.858;DP=1276;FS=21.541;HRun=0;HaplotypeScore=109.0684;InbreedingCoeff=-0.0171;MQ=57.57;MQ0=0;MQRankSum=-14.498;QD=29.97;ReadPosRankSum=1.598;EFF=INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),INTRON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,intron_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 -chr1 1163964 . G A 5411.83 . AC=5;AF=0.0417;AN=120;BaseQRankSum=1.794;DP=3215;Dels=0.00;FS=17.078;HRun=0;HaplotypeScore=1.6463;InbreedingCoeff=0.3739;MQ=58.93;MQ0=0;MQRankSum=1.765;QD=20.19;ReadPosRankSum=-3.159;EFF=EXON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),EXON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D70|202|SDF4|protein_coding|CODING|ENST00000545427|),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D70|348|SDF4|protein_coding|CODING|ENST00000263741|),SYNONYMOUS_CODING(LOW|SILENT|gaC/gaT|D70|362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=synonymous_variant|gaC/gaT|D|ENSG00000078808|SDF4|ENST00000403997|1/5|||5/243|protein_coding|1,synonymous_variant&NMD_transcript_variant|gaC/gaT|D|ENSG00000078808|SDF4|ENST00000465727|2/7|||70/254|nonsense_mediated_decay|1,synonymous_variant|gaC/gaT|D|ENSG00000078808|SDF4|ENST00000360001|2/7|||70/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|2/3||||processed_transcript|1,synonymous_variant|gaC/gaT|D|ENSG00000078808|SDF4|ENST00000545427|2/5|||70/202|protein_coding|1,synonymous_variant|gaC/gaT|D|ENSG00000078808|SDF4|ENST00000263741|2/7|||70/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 -chr1 1164031 . A G 2189.26 . AC=3;AF=0.0250;AN=120;BaseQRankSum=0.524;DP=3658;DS;Dels=0.00;FS=1.511;HRun=0;HaplotypeScore=1.4260;InbreedingCoeff=0.6581;MQ=58.98;MQ0=0;MQRankSum=-0.494;QD=25.46;ReadPosRankSum=2.486;EFF=EXON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),EXON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTa/gCa|V48A|202|SDF4|protein_coding|CODING|ENST00000545427|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTa/gCa|V48A|348|SDF4|protein_coding|CODING|ENST00000263741|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTa/gCa|V48A|362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,missense_variant&NMD_transcript_variant|gTa/gCa|V/A|ENSG00000078808|SDF4|ENST00000465727|2/7|benign(0.002)|tolerated(0.47)|48/254|nonsense_mediated_decay|1,missense_variant|gTa/gCa|V/A|ENSG00000078808|SDF4|ENST00000360001|2/7|benign(0)|tolerated(1)|48/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|2/3||||processed_transcript|1,missense_variant|gTa/gCa|V/A|ENSG00000078808|SDF4|ENST00000545427|2/5|benign(0.002)|tolerated(1)|48/202|protein_coding|1,missense_variant|gTa/gCa|V/A|ENSG00000078808|SDF4|ENST00000263741|2/7|benign(0.002)|tolerated(1)|48/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 -chr1 1164072 . C T 1571.76 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-2.716;DP=2924;DS;Dels=0.00;FS=4.033;HRun=0;HaplotypeScore=1.7879;InbreedingCoeff=0.6581;MQ=59.08;MQ0=0;MQRankSum=-0.133;QD=23.11;ReadPosRankSum=3.483;EFF=EXON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),EXON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),SYNONYMOUS_CODING(LOW|SILENT|gcG/gcA|A34|202|SDF4|protein_coding|CODING|ENST00000545427|),SYNONYMOUS_CODING(LOW|SILENT|gcG/gcA|A34|348|SDF4|protein_coding|CODING|ENST00000263741|),SYNONYMOUS_CODING(LOW|SILENT|gcG/gcA|A34|362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,synonymous_variant&NMD_transcript_variant|gcG/gcA|A|ENSG00000078808|SDF4|ENST00000465727|2/7|||34/254|nonsense_mediated_decay|1,synonymous_variant|gcG/gcA|A|ENSG00000078808|SDF4|ENST00000360001|2/7|||34/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|2/3||||processed_transcript|1,synonymous_variant|gcG/gcA|A|ENSG00000078808|SDF4|ENST00000545427|2/5|||34/202|protein_coding|1,synonymous_variant|gcG/gcA|A|ENSG00000078808|SDF4|ENST00000263741|2/7|||34/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 -chr1 1164090 . T C 1638.23 . AC=3;AF=0.0250;AN=120;BaseQRankSum=0.539;DP=2459;Dels=0.00;FS=3.268;HRun=1;HaplotypeScore=1.1248;InbreedingCoeff=0.6578;MQ=59.09;MQ0=0;MQRankSum=-1.281;QD=25.20;ReadPosRankSum=2.166;EFF=EXON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2),EXON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),SYNONYMOUS_CODING(LOW|SILENT|gcA/gcG|A28|202|SDF4|protein_coding|CODING|ENST00000545427|),SYNONYMOUS_CODING(LOW|SILENT|gcA/gcG|A28|348|SDF4|protein_coding|CODING|ENST00000263741|),SYNONYMOUS_CODING(LOW|SILENT|gcA/gcG|A28|362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,synonymous_variant&NMD_transcript_variant|gcA/gcG|A|ENSG00000078808|SDF4|ENST00000465727|2/7|||28/254|nonsense_mediated_decay|1,synonymous_variant|gcA/gcG|A|ENSG00000078808|SDF4|ENST00000360001|2/7|||28/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|2/3||||processed_transcript|1,synonymous_variant|gcA/gcG|A|ENSG00000078808|SDF4|ENST00000545427|2/5|||28/202|protein_coding|1,synonymous_variant|gcA/gcG|A|ENSG00000078808|SDF4|ENST00000263741|2/7|||28/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 -chr1 1164179 . G A 833.67 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-0.622;DP=1063;Dels=0.00;FS=8.157;HRun=0;HaplotypeScore=0.7297;InbreedingCoeff=0.6212;MQ=58.97;MQ0=0;MQRankSum=1.079;QD=26.89;ReadPosRankSum=2.348;EFF=EXON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|),UTR_5_PRIME(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),UTR_5_PRIME(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),UTR_5_PRIME(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),UTR_5_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,5_prime_UTR_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727|2/7|||-/254|nonsense_mediated_decay|1,5_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000360001|2/7|||-/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|2/3||||processed_transcript|1,5_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000545427|2/5|||-/202|protein_coding|1,5_prime_UTR_variant|||ENSG00000078808|SDF4|ENST00000263741|2/7|||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 -chr1 1164250 . C CCA 210.26 . AC=2;AF=0.0167;AN=120;BaseQRankSum=0.248;DP=413;FS=0.000;HRun=0;HaplotypeScore=29.3469;InbreedingCoeff=0.3092;MQ=58.65;MQ0=0;MQRankSum=2.358;QD=26.28;ReadPosRankSum=0.652;EFF=EXON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|2),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|),UTR_5_PRIME(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|2),UTR_5_PRIME(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|2),UTR_5_PRIME(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|2),UTR_5_PRIME(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|2);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,5_prime_UTR_variant&NMD_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000465727|2/7|||-/254|nonsense_mediated_decay|1,5_prime_UTR_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000360001|2/7|||-/362|protein_coding|1,non_coding_exon_variant&nc_transcript_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000459994|2/3||||processed_transcript|1,5_prime_UTR_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000545427|2/5|||-/202|protein_coding|1,5_prime_UTR_variant&feature_elongation|||ENSG00000078808|SDF4|ENST00000263741|2/7|||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 -chr1 1164687 . G C 63.9 . AC=4;AF=0.118;AN=34;BaseQRankSum=1.043;DP=28;Dels=0.00;FS=10.726;HRun=0;HaplotypeScore=0.2350;InbreedingCoeff=0.1906;MQ=58.33;MQ0=0;MQRankSum=0.241;QD=21.30;ReadPosRankSum=-1.685;EFF=INTRON(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|1),INTRON(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|1),INTRON(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|1),INTRON(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|1),INTRON(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|1),UPSTREAM(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,intron_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,intron_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,intron_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,upstream_gene_variant|||ENSG00000176022|B3GALT6|ENST00000379198||||-/329|protein_coding|1 -chr1 1168180 . G C 380.06 . AC=9;AF=0.105;AN=86;BaseQRankSum=0.307;DP=108;Dels=0.00;FS=6.481;HRun=3;HaplotypeScore=0.2416;InbreedingCoeff=0.2106;MQ=56.49;MQ0=0;MQRankSum=1.066;QD=21.11;ReadPosRankSum=-1.425;EFF=NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gaG/gaC|E174D|329|B3GALT6|protein_coding|CODING|ENST00000379198|),UPSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),UPSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),UPSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),UPSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,missense_variant|gaG/gaC|E/D|ENSG00000176022|B3GALT6|ENST00000379198|1/1|benign(0.012)|tolerated(0.54)|174/329|protein_coding|1 -chr1 1168567 . G A 197.95 . AC=1;AF=0.0098;AN=102;BaseQRankSum=-1.549;DP=173;Dels=0.00;FS=11.564;HRun=1;HaplotypeScore=0.1898;InbreedingCoeff=-0.1063;MQ=57.97;MQ0=0;MQRankSum=0.710;QD=15.23;ReadPosRankSum=-0.901;EFF=SYNONYMOUS_CODING(LOW|SILENT|gaG/gaA|E303|329|B3GALT6|protein_coding|CODING|ENST00000379198|),UPSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),UPSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),UPSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),UPSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,synonymous_variant|gaG/gaA|E|ENSG00000176022|B3GALT6|ENST00000379198|1/1|||303/329|protein_coding|1 -chr1 1168843 . C T 395.96 . AC=2;AF=0.031;AN=64;BaseQRankSum=1.429;DP=826;Dels=0.00;FS=1.955;HRun=0;HaplotypeScore=1.3582;InbreedingCoeff=-0.0815;MQ=56.66;MQ0=0;MQRankSum=0.858;QD=9.90;ReadPosRankSum=0.063;EFF=UPSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),UPSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),UPSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),UPSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),UTR_3_PRIME(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|1);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000403997||||-/243|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,3_prime_UTR_variant|||ENSG00000176022|B3GALT6|ENST00000379198|1/1|||-/329|protein_coding|1 -chr1 1169094 . G C 1587.89 . AC=1;AF=0.016;AN=62;BaseQRankSum=1.089;DP=2518;Dels=0.00;FS=10.659;HRun=0;HaplotypeScore=2.4242;InbreedingCoeff=-0.0613;MQ=58.81;MQ0=0;MQRankSum=1.439;QD=15.72;ReadPosRankSum=-1.308;EFF=UPSTREAM(MODIFIER||||202|SDF4|protein_coding|CODING|ENST00000545427|),UPSTREAM(MODIFIER||||348|SDF4|protein_coding|CODING|ENST00000263741|),UPSTREAM(MODIFIER||||362|SDF4|protein_coding|CODING|ENST00000360001|),UPSTREAM(MODIFIER|||||SDF4|nonsense_mediated_decay|CODING|ENST00000465727|),UPSTREAM(MODIFIER|||||SDF4|processed_transcript|CODING|ENST00000459994|),UTR_3_PRIME(MODIFIER||||329|B3GALT6|protein_coding|CODING|ENST00000379198|1);CSQ=upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000465727||||-/254|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000360001||||-/362|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000459994|||||processed_transcript|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000545427||||-/202|protein_coding|1,upstream_gene_variant|||ENSG00000078808|SDF4|ENST00000263741||||-/348|protein_coding|1,3_prime_UTR_variant|||ENSG00000176022|B3GALT6|ENST00000379198|1/1|||-/329|protein_coding|1 -chr1 1177474 . A G 43.95 . AC=4;AF=0.105;AN=38;BaseQRankSum=-0.867;DP=27;Dels=0.00;FS=0.000;HRun=3;HaplotypeScore=0.0000;InbreedingCoeff=0.1376;MQ=58.27;MQ0=0;MQRankSum=0.685;QD=14.65;ReadPosRankSum=1.780;EFF=DOWNSTREAM(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),INTERGENIC(MODIFIER|||||||||);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1177630 . A G 581.54 . AC=7;AF=0.092;AN=76;BaseQRankSum=-0.178;DP=115;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.1257;InbreedingCoeff=0.0962;MQ=57.55;MQ0=0;MQRankSum=0.915;QD=24.23;ReadPosRankSum=0.465;EFF=DOWNSTREAM(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),INTERGENIC(MODIFIER|||||||||);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1177773 . C G 1999.45 . AC=2;AF=0.0179;AN=112;BaseQRankSum=0.305;DP=963;Dels=0.00;FS=3.764;HRun=0;HaplotypeScore=0.6634;InbreedingCoeff=0.3008;MQ=56.96;MQ0=0;MQRankSum=1.294;QD=32.25;ReadPosRankSum=-0.562;EFF=DOWNSTREAM(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),INTERGENIC(MODIFIER|||||||||);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1177893 . C T 2555.63 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.263;DP=3336;Dels=0.00;FS=2.685;HRun=0;HaplotypeScore=1.5161;InbreedingCoeff=-0.0315;MQ=56.80;MQ0=0;MQRankSum=1.301;QD=12.84;ReadPosRankSum=-1.150;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|3),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|2),UTR_3_PRIME(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|8);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|3/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000184163|FAM132A|ENST00000330388|8/8|||-/302|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000478606|2/2||||retained_intron|1 -chr1 1177918 . CT C 3669.24 . AC=4;AF=0.0333;AN=120;BaseQRankSum=7.568;DP=3999;DS;FS=3.238;HRun=1;HaplotypeScore=287.5611;InbreedingCoeff=-0.0451;MQ=56.98;MQ0=0;MQRankSum=0.885;QD=11.87;ReadPosRankSum=-0.015;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|3),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|2),UTR_3_PRIME(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|8);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000184163|FAM132A|ENST00000462849|3/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,3_prime_UTR_variant&feature_truncation|||ENSG00000184163|FAM132A|ENST00000330388|8/8|||-/302|protein_coding|1,non_coding_exon_variant&nc_transcript_variant&feature_truncation|||ENSG00000184163|FAM132A|ENST00000478606|2/2||||retained_intron|1 -chr1 1177919 . T C 61.88 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-3.136;DP=3999;DS;Dels=0.03;FS=1.983;HRun=6;HaplotypeScore=4.0470;InbreedingCoeff=-0.0307;MQ=56.98;MQ0=0;MQRankSum=0.755;QD=0.41;ReadPosRankSum=-5.282;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|3),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|2),UTR_3_PRIME(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|8);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|3/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000184163|FAM132A|ENST00000330388|8/8|||-/302|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000478606|2/2||||retained_intron|1 -chr1 1178047 . T C 1725.37 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.230;DP=3874;DS;Dels=0.00;FS=5.482;HRun=1;HaplotypeScore=3.6848;InbreedingCoeff=-0.0204;MQ=57.02;MQ0=0;MQRankSum=-0.683;QD=10.59;ReadPosRankSum=1.820;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|7),INTRON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|2),INTRON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|1);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1178244 . G C 483.23 . AC=2;AF=0.0167;AN=120;BaseQRankSum=1.151;DP=1605;Dels=0.00;FS=2.186;HRun=1;HaplotypeScore=1.4521;InbreedingCoeff=0.7073;MQ=59.01;MQ0=0;MQRankSum=0.273;QD=32.22;ReadPosRankSum=-0.751;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|2),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cta/Gta|L261V|302|FAM132A|protein_coding|CODING|ENST00000330388|);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|2/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,missense_variant|Cta/Gta|L/V|ENSG00000184163|FAM132A|ENST00000330388|7/8|benign(0.001)|tolerated(0.98)|261/302|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000478606|1/2||||retained_intron|1 -chr1 1178245 . C T 4066.47 . AC=15;AF=0.1250;AN=120;BaseQRankSum=-1.548;DP=1603;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.3636;InbreedingCoeff=0.1530;MQ=59.01;MQ0=0;MQRankSum=1.224;QD=13.51;ReadPosRankSum=-1.253;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|2),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|1),SYNONYMOUS_CODING(LOW|SILENT|acG/acA|T260|302|FAM132A|protein_coding|CODING|ENST00000330388|);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|2/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,synonymous_variant|acG/acA|T|ENSG00000184163|FAM132A|ENST00000330388|7/8|||260/302|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000478606|1/2||||retained_intron|1 -chr1 1178453 . G A 466.91 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-3.075;DP=1354;Dels=0.00;FS=5.034;HRun=0;HaplotypeScore=0.8964;InbreedingCoeff=0.6716;MQ=57.75;MQ0=0;MQRankSum=1.619;QD=23.35;ReadPosRankSum=0.909;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|1),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|1),SYNONYMOUS_CODING(LOW|SILENT|tgC/tgT|C240|302|FAM132A|protein_coding|CODING|ENST00000330388|);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,synonymous_variant|tgC/tgT|C|ENSG00000184163|FAM132A|ENST00000330388|6/8|||240/302|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000478606|1/2||||retained_intron|1 -chr1 1178482 . A G 2936.1 . AC=13;AF=0.1121;AN=116;BaseQRankSum=2.434;DP=1386;Dels=0.00;FS=2.627;HRun=0;HaplotypeScore=0.8846;InbreedingCoeff=0.1962;MQ=57.58;MQ0=0;MQRankSum=-0.397;QD=11.84;ReadPosRankSum=1.190;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|1),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|1),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Tgt/Cgt|C231R|302|FAM132A|protein_coding|CODING|ENST00000330388|);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,missense_variant|Tgt/Cgt|C/R|ENSG00000184163|FAM132A|ENST00000330388|6/8|benign(0)|tolerated(1)|231/302|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000478606|1/2||||retained_intron|1 -chr1 1178571 . C G 79.76 . AC=15;AF=0.1364;AN=110;BaseQRankSum=-6.811;DP=485;Dels=0.00;FS=26.762;HRun=6;HaplotypeScore=2.2448;InbreedingCoeff=-0.0971;MQ=52.03;MQ0=0;MQRankSum=-8.725;QD=0.69;ReadPosRankSum=1.393;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|1),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|5),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1178576 . T G 181.67 . AC=17;AF=0.1574;AN=108;BaseQRankSum=-5.916;DP=438;Dels=0.01;FS=1.537;HRun=5;HaplotypeScore=3.1212;InbreedingCoeff=-0.0423;MQ=51.34;MQ0=0;MQRankSum=-4.551;QD=1.36;ReadPosRankSum=1.353;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|1),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|5),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1178586 . T G 169.37 . AC=15;AF=0.150;AN=100;BaseQRankSum=-5.907;DP=387;Dels=0.00;FS=26.401;HRun=4;HaplotypeScore=3.0709;InbreedingCoeff=-0.2409;MQ=49.88;MQ0=0;MQRankSum=-7.180;QD=1.44;ReadPosRankSum=1.347;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|1),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|5),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000462849|1/3||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1178925 . G A 1946.86 . AC=13;AF=0.1102;AN=118;BaseQRankSum=-1.965;DP=1010;Dels=0.00;FS=8.294;HRun=0;HaplotypeScore=0.9315;InbreedingCoeff=0.1268;MQ=57.94;MQ0=0;MQRankSum=0.962;QD=13.91;ReadPosRankSum=-0.443;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|3),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCc/gTc|A180V|302|FAM132A|protein_coding|CODING|ENST00000330388|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000468365|3/3||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,missense_variant|gCc/gTc|A/V|ENSG00000184163|FAM132A|ENST00000330388|5/8|possibly_damaging(0.558)|tolerated(0.06)|180/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1178935 . G A 255.43 . AC=1;AF=0.0085;AN=118;BaseQRankSum=0.108;DP=915;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6695;InbreedingCoeff=-0.0264;MQ=58.14;MQ0=0;MQRankSum=0.476;QD=14.19;ReadPosRankSum=1.215;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|4),INTRON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|2),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1179030 . G A 765.15 . AC=11;AF=0.0982;AN=112;BaseQRankSum=-0.728;DP=324;Dels=0.00;FS=3.299;HRun=0;HaplotypeScore=0.2197;InbreedingCoeff=0.1064;MQ=58.27;MQ0=0;MQRankSum=-0.685;QD=17.39;ReadPosRankSum=0.230;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|4),INTRON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|2),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1179309 . G A 1486.6 . AC=12;AF=0.1017;AN=118;BaseQRankSum=-0.202;DP=527;Dels=0.00;FS=5.698;HRun=0;HaplotypeScore=0.2810;InbreedingCoeff=0.1431;MQ=57.09;MQ0=0;MQRankSum=0.038;QD=15.65;ReadPosRankSum=-0.459;EFF=DOWNSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|4),INTRON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|2),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,downstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1179416 . A C 387.28 . AC=8;AF=0.0690;AN=116;BaseQRankSum=-8.770;DP=848;Dels=0.00;FS=181.958;HRun=3;HaplotypeScore=0.9454;InbreedingCoeff=-0.0952;MQ=53.74;MQ0=0;MQRankSum=-2.436;QD=3.25;ReadPosRankSum=-1.967;EFF=EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|2),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|2),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gTg/gGg|V150G|302|FAM132A|protein_coding|CODING|ENST00000330388|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000468365|2/3||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000486627|2/2||||retained_intron|1,missense_variant|gTg/gGg|V/G|ENSG00000184163|FAM132A|ENST00000330388|4/8|possibly_damaging(0.839)|deleterious(0)|150/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1179532 . C T 490.97 . AC=2;AF=0.0167;AN=120;BaseQRankSum=1.132;DP=1835;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.0586;InbreedingCoeff=-0.0210;MQ=57.90;MQ0=0;MQRankSum=1.423;QD=10.45;ReadPosRankSum=0.071;EFF=EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|2),EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|2),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|3),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000468365|2/3||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000486627|2/2||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1179747 . C T 48.72 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.106;DP=2070;Dels=0.00;FS=5.993;HRun=0;HaplotypeScore=1.5289;InbreedingCoeff=-0.0284;MQ=59.19;MQ0=0;MQRankSum=-1.469;QD=6.96;ReadPosRankSum=1.335;EFF=INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|2),INTRON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|1),INTRON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|1),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=intron_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,intron_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1179800 . G A 782.26 . AC=2;AF=0.0172;AN=116;BaseQRankSum=-3.138;DP=2073;Dels=0.00;FS=2.190;HRun=0;HaplotypeScore=1.3315;InbreedingCoeff=-0.0420;MQ=59.44;MQ0=0;MQRankSum=-0.175;QD=9.78;ReadPosRankSum=0.512;EFF=EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|1),SYNONYMOUS_CODING(LOW|SILENT|ggC/ggT|G85|302|FAM132A|protein_coding|CODING|ENST00000330388|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000486627|1/2||||retained_intron|1,synonymous_variant|ggC/ggT|G|ENSG00000184163|FAM132A|ENST00000330388|2/8|||85/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1179983 . G A 2866.16 . AC=17;AF=0.1417;AN=120;BaseQRankSum=-1.868;DP=688;Dels=0.00;FS=0.855;HRun=0;HaplotypeScore=0.3402;InbreedingCoeff=0.3638;MQ=59.13;MQ0=0;MQRankSum=0.378;QD=21.71;ReadPosRankSum=-2.062;EFF=EXON(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|1),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|1),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|);CSQ=upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000184163|FAM132A|ENST00000486627|1/2||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1181372 . C T 142.78 . AC=9;AF=0.205;AN=44;BaseQRankSum=0.940;DP=56;Dels=0.00;FS=10.677;HRun=0;HaplotypeScore=0.1214;InbreedingCoeff=0.3107;MQ=57.48;MQ0=0;MQRankSum=0.070;QD=20.40;ReadPosRankSum=1.361;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|1),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|);CSQ=upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,downstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1181751 . T C 107.96 . AC=6;AF=0.130;AN=46;BaseQRankSum=0.029;DP=35;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0398;InbreedingCoeff=0.0106;MQ=56.62;MQ0=0;MQRankSum=0.202;QD=15.42;ReadPosRankSum=0.375;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),INTRON(MODIFIER||||302|FAM132A|protein_coding|CODING|ENST00000330388|1),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000462849|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000468365|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000478606|),UPSTREAM(MODIFIER|||||FAM132A|retained_intron|CODING|ENST00000486627|);CSQ=upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000468365|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000462849|||||retained_intron|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000486627|||||retained_intron|1,intron_variant|||ENSG00000184163|FAM132A|ENST00000330388||||-/302|protein_coding|1,downstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,upstream_gene_variant|||ENSG00000184163|FAM132A|ENST00000478606|||||retained_intron|1 -chr1 1190061 . A G 343.05 . AC=12;AF=0.176;AN=68;BaseQRankSum=1.182;DP=113;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.0935;InbreedingCoeff=0.3239;MQ=57.93;MQ0=0;MQRankSum=0.135;QD=20.18;ReadPosRankSum=-0.224;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|),DOWNSTREAM(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|),DOWNSTREAM(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|),DOWNSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),DOWNSTREAM(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|),DOWNSTREAM(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|7),UTR_3_PRIME(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|7),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|8);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000349431|7/7|||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|7/7|||-/207|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390|8/8|||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1190166 . G A 197.18 . AC=1;AF=0.017;AN=58;BaseQRankSum=1.290;DP=332;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.5654;InbreedingCoeff=-0.0510;MQ=56.96;MQ0=0;MQRankSum=0.784;QD=10.38;ReadPosRankSum=-0.832;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|),DOWNSTREAM(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|),DOWNSTREAM(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|),DOWNSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),DOWNSTREAM(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|),DOWNSTREAM(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|7),UTR_3_PRIME(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|7),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|8);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000349431|7/7|||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|7/7|||-/207|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390|8/8|||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1190206 . G A 197.94 . AC=1;AF=0.015;AN=68;BaseQRankSum=-0.934;DP=520;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7388;InbreedingCoeff=-0.0641;MQ=57.17;MQ0=0;MQRankSum=2.013;QD=15.23;ReadPosRankSum=-0.458;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|),DOWNSTREAM(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|),DOWNSTREAM(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|),DOWNSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),DOWNSTREAM(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|),DOWNSTREAM(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|7),UTR_3_PRIME(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|7),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|8);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000349431|7/7|||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|7/7|||-/207|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390|8/8|||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1190207 . C T 432.13 . AC=7;AF=0.100;AN=70;BaseQRankSum=-1.418;DP=529;Dels=0.00;FS=4.930;HRun=0;HaplotypeScore=0.6967;InbreedingCoeff=0.1975;MQ=57.18;MQ0=0;MQRankSum=0.339;QD=9.39;ReadPosRankSum=1.134;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|),DOWNSTREAM(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|),DOWNSTREAM(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|),DOWNSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),DOWNSTREAM(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|),DOWNSTREAM(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|7),UTR_3_PRIME(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|7),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|8);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000349431|7/7|||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|7/7|||-/207|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390|8/8|||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1190481 . G A 116.73 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.156;DP=714;Dels=0.00;FS=12.015;HRun=1;HaplotypeScore=0.6509;InbreedingCoeff=-0.0173;MQ=59.06;MQ0=0;MQRankSum=-0.238;QD=6.49;ReadPosRankSum=1.991;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|),DOWNSTREAM(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|),DOWNSTREAM(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|7),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|7),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|6),UTR_3_PRIME(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|6),UTR_3_PRIME(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|7),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|8),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|8);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000339385|6/6|||-/224|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036|8/8|||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000349431|7/7|||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000348298|7/7|||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|7/7|||-/207|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390|8/8|||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000400929|6/6|||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1190523 . C T 321.56 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-1.643;DP=1091;Dels=0.00;FS=3.027;HRun=0;HaplotypeScore=0.7506;InbreedingCoeff=-0.0300;MQ=58.89;MQ0=0;MQRankSum=-0.522;QD=7.31;ReadPosRankSum=-0.760;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|),DOWNSTREAM(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|),DOWNSTREAM(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|7),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|7),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|6),UTR_3_PRIME(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|6),UTR_3_PRIME(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|7),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|8),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|8);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000339385|6/6|||-/224|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036|8/8|||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000349431|7/7|||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000348298|7/7|||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|7/7|||-/207|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390|8/8|||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000400929|6/6|||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1190544 . G A 120.24 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.624;DP=1337;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.7866;InbreedingCoeff=-0.0117;MQ=58.85;MQ0=0;MQRankSum=-0.513;QD=4.01;ReadPosRankSum=1.551;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|),DOWNSTREAM(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|7),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|7),UTR_3_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|6),UTR_3_PRIME(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|6),UTR_3_PRIME(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|7),UTR_3_PRIME(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|7),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|8),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|8);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000339385|6/6|||-/224|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036|8/8|||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000349431|7/7|||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000348298|7/7|||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|7/7|||-/207|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390|8/8|||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000400929|6/6|||-/207|protein_coding|1,3_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000360466|7/7|||-/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1190685 . G A 695.66 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.799;DP=3117;Dels=0.00;FS=3.835;HRun=0;HaplotypeScore=1.7262;InbreedingCoeff=-0.0084;MQ=59.01;MQ0=0;MQRankSum=1.046;QD=13.38;ReadPosRankSum=-1.664;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),EXON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|2),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H174|207|UBE2J2|protein_coding|CODING|ENST00000347370|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H174|207|UBE2J2|protein_coding|CODING|ENST00000348298|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H174|207|UBE2J2|protein_coding|CODING|ENST00000400929|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H191|224|UBE2J2|protein_coding|CODING|ENST00000339385|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H226|259|UBE2J2|protein_coding|CODING|ENST00000349431|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H226|259|UBE2J2|protein_coding|CODING|ENST00000360466|),SYNONYMOUS_CODING(LOW|SILENT|caC/caT|H242|275|UBE2J2|protein_coding|CODING|ENST00000400930|),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|8),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|8),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|7);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,synonymous_variant|caC/caT|H|ENSG00000160087|UBE2J2|ENST00000339385|6/6|||191/224|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215|7/7|||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036|8/8|||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,synonymous_variant|caC/caT|H|ENSG00000160087|UBE2J2|ENST00000349431|7/7|||226/259|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000467339|2/2||||processed_transcript|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,synonymous_variant|caC/caT|H|ENSG00000160087|UBE2J2|ENST00000348298|7/7|||174/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,synonymous_variant|caC/caT|H|ENSG00000160087|UBE2J2|ENST00000347370|7/7|||174/207|protein_coding|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390|8/8|||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,synonymous_variant|caC/caT|H|ENSG00000160087|UBE2J2|ENST00000400930|8/8|||242/275|protein_coding|1,synonymous_variant|caC/caT|H|ENSG00000160087|UBE2J2|ENST00000400929|6/6|||174/207|protein_coding|1,synonymous_variant|caC/caT|H|ENSG00000160087|UBE2J2|ENST00000360466|7/7|||226/259|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1190913 . CT C 3484.23 . AC=11;AF=0.0917;AN=120;BaseQRankSum=10.114;DP=1539;FS=1.866;HRun=3;HaplotypeScore=78.4459;InbreedingCoeff=0.1110;MQ=58.86;MQ0=0;MQRankSum=0.337;QD=15.98;ReadPosRankSum=0.968;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|6),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|6),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|6),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|5),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|5),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|6),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|6),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|7),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|7),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|7),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|4),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|1),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1191290 . C T 273.07 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.037;DP=1868;Dels=0.00;FS=4.676;HRun=1;HaplotypeScore=1.1539;InbreedingCoeff=-0.0090;MQ=58.69;MQ0=0;MQRankSum=0.087;QD=11.38;ReadPosRankSum=0.846;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|6),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|6),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|6),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|5),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|5),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|6),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|6),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|7),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|7),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|7),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|4),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|1),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1191589 . G A 716.56 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.146;DP=2848;Dels=0.00;FS=11.254;HRun=0;HaplotypeScore=1.5890;InbreedingCoeff=-0.0084;MQ=59.49;MQ0=0;MQRankSum=0.568;QD=9.55;ReadPosRankSum=0.273;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),EXON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|1),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|4),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|4),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|5),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|5),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|3),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|3),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000467339|1/2||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1191651 . A G 329.19 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.637;DP=1304;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5306;InbreedingCoeff=-0.0104;MQ=59.58;MQ0=0;MQRankSum=0.314;QD=16.46;ReadPosRankSum=1.231;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),EXON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|1),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|4),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|4),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|5),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|5),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|3),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|3),UPSTREAM(MODIFIER|||||RP5-902P8.12|lincRNA|NON_CODING|ENST00000565563|);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000260179|RP5-902P8.12|ENST00000565563|||||lincRNA|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000467339|1/2||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192105 . C A 38.15 . AC=5;AF=0.081;AN=62;BaseQRankSum=0.672;DP=78;Dels=0.00;FS=4.123;HRun=11;HaplotypeScore=0.3295;InbreedingCoeff=-0.0659;MQ=39.13;MQ0=0;MQRankSum=-1.521;QD=4.77;ReadPosRankSum=-0.389;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|4),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|4),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|5),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|5),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|3),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|3),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192268 . G A 34.31 . AC=9;AF=0.0763;AN=118;BaseQRankSum=-2.951;DP=712;Dels=0.00;FS=86.476;HRun=23;HaplotypeScore=4.3915;InbreedingCoeff=-0.1175;MQ=40.22;MQ0=0;MQRankSum=-4.056;QD=0.35;ReadPosRankSum=-6.599;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|4),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|4),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|5),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|5),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|3),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|3),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192321 . C A 197.5 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.375;DP=1985;Dels=0.00;FS=2.097;HRun=0;HaplotypeScore=2.7167;InbreedingCoeff=-0.0116;MQ=52.62;MQ0=0;MQRankSum=0.704;QD=10.97;ReadPosRankSum=-0.366;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|),DOWNSTREAM(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|),DOWNSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|5),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|4),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|4),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|5),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|5),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|6),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|3),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|3),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192515 . A G 9144.94 . AC=10;AF=0.0833;AN=120;BaseQRankSum=-2.796;DP=4520;Dels=0.00;FS=4.056;HRun=0;HaplotypeScore=2.8736;InbreedingCoeff=0.1273;MQ=57.88;MQ0=0;MQRankSum=7.716;QD=16.19;ReadPosRankSum=-1.141;EFF=DOWNSTREAM(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|),DOWNSTREAM(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|4),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|4),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|4),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|3),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|4),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|4),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|5),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|2),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|2),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192767 . C G 279.19 . AC=5;AF=0.0424;AN=118;BaseQRankSum=-0.503;DP=1028;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=10.9757;InbreedingCoeff=-0.0595;MQ=43.48;MQ0=0;MQRankSum=0.689;QD=3.49;ReadPosRankSum=-0.850;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192769 . CG C 46.23 . AC=14;AF=0.1167;AN=120;BaseQRankSum=7.490;DP=929;FS=8.084;HRun=1;HaplotypeScore=182.4593;InbreedingCoeff=-0.1268;MQ=41.55;MQ0=0;MQRankSum=-2.659;QD=0.18;ReadPosRankSum=0.739;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192770 . GCCA G 709.55 . AC=36;AF=0.3051;AN=118;BaseQRankSum=7.682;DP=910;FS=3.236;HRun=0;HaplotypeScore=185.0135;InbreedingCoeff=-0.3195;MQ=40.92;MQ0=0;MQRankSum=-5.310;QD=1.24;ReadPosRankSum=5.795;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192771 . CCA C 1171.87 . AC=44;AF=0.3729;AN=118;BaseQRankSum=10.317;DP=897;FS=2.917;HRun=0;HaplotypeScore=180.9024;InbreedingCoeff=-0.3867;MQ=40.58;MQ0=0;MQRankSum=-6.072;QD=1.66;ReadPosRankSum=5.982;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192772 . CA C 1504.33 . AC=45;AF=0.3879;AN=116;BaseQRankSum=10.581;DP=873;FS=9.989;HRun=1;HaplotypeScore=179.8239;InbreedingCoeff=-0.4727;MQ=39.95;MQ0=0;MQRankSum=-6.732;QD=2.06;ReadPosRankSum=6.533;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192773 . AC A 268.82 . AC=52;AF=0.4815;AN=108;BaseQRankSum=1.061;DP=867;FS=7.111;HRun=15;HaplotypeScore=182.8712;InbreedingCoeff=0.1639;MQ=39.70;MQ0=0;MQRankSum=-1.275;QD=0.46;ReadPosRankSum=4.858;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant&feature_truncation|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192776 . C G 174.83 . AC=3;AF=0.0273;AN=110;BaseQRankSum=-0.288;DP=833;Dels=0.01;FS=0.000;HRun=0;HaplotypeScore=8.8403;InbreedingCoeff=-0.0771;MQ=38.73;MQ0=0;MQRankSum=2.194;QD=3.64;ReadPosRankSum=-1.689;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192786 . C A 471.53 . AC=12;AF=0.1154;AN=104;BaseQRankSum=-6.703;DP=722;Dels=0.00;FS=2.215;HRun=0;HaplotypeScore=1.5194;InbreedingCoeff=0.1443;MQ=35.94;MQ0=0;MQRankSum=0.095;QD=3.27;ReadPosRankSum=2.115;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192786 . C CA 288.17 . AC=16;AF=0.1455;AN=110;BaseQRankSum=0.649;DP=716;FS=2.212;HRun=0;HaplotypeScore=181.3815;InbreedingCoeff=0.2496;MQ=36.10;MQ0=0;MQRankSum=-1.597;QD=1.84;ReadPosRankSum=-0.511;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192787 . C G 453.3 . AC=12;AF=0.120;AN=100;BaseQRankSum=2.173;DP=716;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.4128;InbreedingCoeff=0.1641;MQ=36.10;MQ0=0;MQRankSum=-0.721;QD=4.08;ReadPosRankSum=0.454;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192787 . C CG 374.1 . AC=17;AF=0.1545;AN=110;BaseQRankSum=0.800;DP=712;FS=0.000;HRun=0;HaplotypeScore=181.2272;InbreedingCoeff=0.2381;MQ=36.30;MQ0=0;MQRankSum=-3.098;QD=2.65;ReadPosRankSum=-1.333;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant&feature_elongation|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192789 . T C 74.78 . AC=1;AF=0.0098;AN=102;BaseQRankSum=-1.047;DP=679;Dels=0.00;FS=0.000;HRun=15;HaplotypeScore=1.3798;InbreedingCoeff=-0.0743;MQ=36.78;MQ0=0;MQRankSum=-1.425;QD=9.35;ReadPosRankSum=1.189;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1192796 . G A 212.87 . AC=4;AF=0.0364;AN=110;BaseQRankSum=-1.973;DP=615;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.2937;InbreedingCoeff=-0.0566;MQ=38.68;MQ0=0;MQRankSum=-0.959;QD=4.09;ReadPosRankSum=-2.635;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1),UPSTREAM(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000467339|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000467339|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1198510 . G A 538.65 . AC=2;AF=0.0179;AN=112;BaseQRankSum=2.023;DP=404;Dels=0.00;FS=3.013;HRun=0;HaplotypeScore=0.4208;InbreedingCoeff=-0.0639;MQ=57.33;MQ0=0;MQRankSum=1.298;QD=17.96;ReadPosRankSum=1.405;EFF=DOWNSTREAM(MODIFIER||||42|UBE2J2|protein_coding|CODING|ENST00000488418|),EXON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|3),INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|3/3||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000488418||||-/43|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1198578 . C T 938.59 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-0.880;DP=1202;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.1589;InbreedingCoeff=0.4530;MQ=59.30;MQ0=0;MQRankSum=0.985;QD=21.33;ReadPosRankSum=-2.169;EFF=DOWNSTREAM(MODIFIER||||42|UBE2J2|protein_coding|CODING|ENST00000488418|),EXON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|3),INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|3/3||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000488418||||-/43|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1198618 . T A 9157.74 . AC=14;AF=0.1167;AN=120;BaseQRankSum=0.445;DP=2253;Dels=0.00;FS=1.666;HRun=1;HaplotypeScore=1.0999;InbreedingCoeff=0.1893;MQ=59.34;MQ0=0;MQRankSum=0.163;QD=16.59;ReadPosRankSum=2.122;EFF=DOWNSTREAM(MODIFIER||||42|UBE2J2|protein_coding|CODING|ENST00000488418|),EXON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|3),INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|4),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|3),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|2),INTRON(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|3),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|3),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|4),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|4),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|2),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|1);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|3/3||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000488418||||-/43|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1203116 . G A 525.11 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.823;DP=1403;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.4562;InbreedingCoeff=-0.0106;MQ=58.08;MQ0=0;MQRankSum=2.079;QD=11.93;ReadPosRankSum=2.071;EFF=DOWNSTREAM(MODIFIER||||42|UBE2J2|protein_coding|CODING|ENST00000488418|),INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|2),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|2),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|2),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|1),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|2),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|2),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|1),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|2),UPSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),UPSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|2),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|2),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|2),UTR_5_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|2);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|||||retained_intron|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215|2/7|||-/44|nonsense_mediated_decay|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294|2/6|||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000488418||||-/43|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,5_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|2/7|||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752|2/6|||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1203167 . C T 1087.43 . AC=2;AF=0.0167;AN=120;BaseQRankSum=1.333;DP=2258;Dels=0.00;FS=8.468;HRun=1;HaplotypeScore=1.5625;InbreedingCoeff=0.8365;MQ=57.71;MQ0=0;MQRankSum=-0.200;QD=28.62;ReadPosRankSum=-1.052;EFF=DOWNSTREAM(MODIFIER||||42|UBE2J2|protein_coding|CODING|ENST00000488418|),INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|2),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|2),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|2),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|1),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|2),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|2),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|2),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|3),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|1),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|2),UPSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),UPSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|2),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|2),UTR_3_PRIME(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|2),UTR_5_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|2);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|||||retained_intron|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215|2/7|||-/44|nonsense_mediated_decay|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294|2/6|||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000488418||||-/43|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,5_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|2/7|||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752|2/6|||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1203271 . G A 523.79 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.124;DP=5255;Dels=0.00;FS=0.700;HRun=0;HaplotypeScore=3.4063;InbreedingCoeff=-0.0084;MQ=58.09;MQ0=0;MQRankSum=0.171;QD=10.48;ReadPosRankSum=-1.486;EFF=EXON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|2),EXON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|2),EXON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|2),EXON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|2),EXON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|2),EXON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|2),EXON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|2),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|1),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A34|100|UBE2J2|protein_coding|CODING|ENST00000422076|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A34|195|UBE2J2|protein_coding|CODING|ENST00000435198|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A34|259|UBE2J2|protein_coding|CODING|ENST00000349431|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A34|259|UBE2J2|protein_coding|CODING|ENST00000360466|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A34|275|UBE2J2|protein_coding|CODING|ENST00000400930|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A34|42|UBE2J2|protein_coding|CODING|ENST00000488418|),SYNONYMOUS_CODING(LOW|SILENT|gcC/gcT|A34|70|UBE2J2|protein_coding|CODING|ENST00000502382|),UPSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),UPSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|),UTR_5_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|2),UTR_5_PRIME(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|2);CSQ=synonymous_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000502382|3/5|||34/71|protein_coding|1,synonymous_variant&NMD_transcript_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000477894|2/6|||34/100|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|2/3||||retained_intron|1,synonymous_variant&NMD_transcript_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000473215|2/7|||34/44|nonsense_mediated_decay|1,synonymous_variant&NMD_transcript_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000503294|2/6|||34/44|nonsense_mediated_decay|1,synonymous_variant&NMD_transcript_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000464036|2/8|||34/105|nonsense_mediated_decay|1,synonymous_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000488418|2/2|||34/43|protein_coding|1,synonymous_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000422076|2/5|||34/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,synonymous_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000349431|2/7|||34/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,5_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000348298|2/7|||-/207|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,5_prime_UTR_variant|||ENSG00000160087|UBE2J2|ENST00000347370|2/7|||-/207|protein_coding|1,synonymous_variant&NMD_transcript_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000450390|2/8|||34/43|nonsense_mediated_decay|1,synonymous_variant&NMD_transcript_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000466752|2/6|||34/44|nonsense_mediated_decay|1,synonymous_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000400930|2/8|||34/275|protein_coding|1,synonymous_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000360466|2/7|||34/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,synonymous_variant|gcC/gcT|A|ENSG00000160087|UBE2J2|ENST00000435198|2/7|||34/196|protein_coding|1 -chr1 1203387 . G A 3095.18 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-1.839;DP=3379;Dels=0.00;FS=3.622;HRun=0;HaplotypeScore=1.5906;InbreedingCoeff=0.4826;MQ=57.66;MQ0=0;MQRankSum=0.825;QD=17.10;ReadPosRankSum=1.782;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|1),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|1),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|1),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|1),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|1),INTRON(MODIFIER||||42|UBE2J2|protein_coding|CODING|ENST00000488418|1),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|1),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|1),UPSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),UPSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000488418||||-/43|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1203464 . G A 885.88 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-4.027;DP=1394;Dels=0.00;FS=13.230;HRun=0;HaplotypeScore=0.4057;InbreedingCoeff=-0.0494;MQ=51.99;MQ0=0;MQRankSum=3.105;QD=10.42;ReadPosRankSum=-0.558;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|1),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|1),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|1),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|1),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|1),INTRON(MODIFIER||||42|UBE2J2|protein_coding|CODING|ENST00000488418|1),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|1),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|1),UPSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),UPSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000488418||||-/43|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1203573 . C T 46.79 . AC=2;AF=0.0169;AN=118;BaseQRankSum=-2.029;DP=398;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3328;InbreedingCoeff=-0.0477;MQ=57.94;MQ0=0;MQRankSum=0.064;QD=3.90;ReadPosRankSum=-2.020;EFF=INTRON(MODIFIER||||100|UBE2J2|protein_coding|CODING|ENST00000422076|1),INTRON(MODIFIER||||195|UBE2J2|protein_coding|CODING|ENST00000435198|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000347370|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000348298|1),INTRON(MODIFIER||||207|UBE2J2|protein_coding|CODING|ENST00000400929|1),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000349431|1),INTRON(MODIFIER||||259|UBE2J2|protein_coding|CODING|ENST00000360466|1),INTRON(MODIFIER||||275|UBE2J2|protein_coding|CODING|ENST00000400930|1),INTRON(MODIFIER||||42|UBE2J2|protein_coding|CODING|ENST00000488418|1),INTRON(MODIFIER||||70|UBE2J2|protein_coding|CODING|ENST00000502382|2),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000450390|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000464036|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000466752|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000473215|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000477894|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000503294|1),INTRON(MODIFIER|||||UBE2J2|nonsense_mediated_decay|CODING|ENST00000509720|1),INTRON(MODIFIER|||||UBE2J2|processed_transcript|CODING|ENST00000491779|1),INTRON(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000461142|1),UPSTREAM(MODIFIER||||224|UBE2J2|protein_coding|CODING|ENST00000339385|),UPSTREAM(MODIFIER|||||UBE2J2|retained_intron|CODING|ENST00000471154|);CSQ=intron_variant|||ENSG00000160087|UBE2J2|ENST00000502382||||-/71|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000477894||||-/100|nonsense_mediated_decay|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000339385||||-/224|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000461142|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000473215||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000503294||||-/44|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000464036||||-/105|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000488418||||-/43|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000422076||||-/101|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000491779|||||processed_transcript|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000349431||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000509720||||-/60|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000348298||||-/207|protein_coding|1,upstream_gene_variant|||ENSG00000160087|UBE2J2|ENST00000471154|||||retained_intron|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000347370||||-/207|protein_coding|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000450390||||-/43|nonsense_mediated_decay|1,intron_variant&NMD_transcript_variant|||ENSG00000160087|UBE2J2|ENST00000466752||||-/44|nonsense_mediated_decay|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400930||||-/275|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000360466||||-/259|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000400929||||-/207|protein_coding|1,intron_variant|||ENSG00000160087|UBE2J2|ENST00000435198||||-/196|protein_coding|1 -chr1 1219382 . C G 517.5 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-1.346;DP=1303;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.5737;InbreedingCoeff=-0.0262;MQ=56.33;MQ0=0;MQRankSum=-0.677;QD=16.69;ReadPosRankSum=0.201;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),EXON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),EXON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cag/Gag|Q126E|802|SCNN1D|protein_coding|CODING|ENST00000379116|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cag/Gag|Q28E|704|SCNN1D|protein_coding|CODING|ENST00000325425|),START_GAINED(LOW||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),STOP_GAINED(HIGH|NONSENSE|tCa/tGa|S29*|138|SCNN1D|protein_coding|CODING|ENST00000470022|),UTR_5_PRIME(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),UTR_5_PRIME(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6);CSQ=5_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000338555|3/15|||-/638|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000467651|3/4||||processed_transcript|1,missense_variant&NMD_transcript_variant|Cag/Gag|Q/E|ENSG00000162572|SCNN1D|ENST00000379101|5/17|benign(0.028)||126/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,missense_variant|Cag/Gag|Q/E|ENSG00000162572|SCNN1D|ENST00000325425|2/15|benign(0.007)|deleterious(0)|28/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,stop_gained|tCa/tGa|S/*|ENSG00000162572|SCNN1D|ENST00000470022|3/4|||29/139|protein_coding|1,5_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000400928|2/14|||-/638|protein_coding|1,missense_variant|Cag/Gag|Q/E|ENSG00000162572|SCNN1D|ENST00000379116|5/18|benign(0.007)|deleterious(0)|126/802|protein_coding|1 -chr1 1219477 . T G 61.09 . AC=10;AF=0.0962;AN=104;BaseQRankSum=-2.023;DP=228;Dels=0.02;FS=0.000;HRun=9;HaplotypeScore=1.3848;InbreedingCoeff=0.0086;MQ=48.26;MQ0=0;MQRankSum=-5.204;QD=1.61;ReadPosRankSum=5.116;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,splice_region_variant&intron_variant&nc_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1219487 . T G 252.82 . AC=16;AF=0.178;AN=90;BaseQRankSum=-6.004;DP=169;Dels=0.04;FS=31.669;HRun=9;HaplotypeScore=0.8999;InbreedingCoeff=-0.0964;MQ=41.70;MQ0=0;MQRankSum=-5.947;QD=4.29;ReadPosRankSum=5.203;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1219489 . A G 510.67 . AC=22;AF=0.244;AN=90;BaseQRankSum=-4.579;DP=161;Dels=0.04;FS=3.458;HRun=6;HaplotypeScore=0.5978;InbreedingCoeff=-0.2229;MQ=40.79;MQ0=0;MQRankSum=-5.372;QD=6.01;ReadPosRankSum=7.135;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1219495 . GT G 259.6 . AC=33;AF=0.434;AN=76;BaseQRankSum=1.135;DP=118;FS=0.000;HRun=1;HaplotypeScore=47.6513;InbreedingCoeff=0.0098;MQ=32.06;MQ0=0;MQRankSum=-2.879;QD=3.05;ReadPosRankSum=1.806;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1219503 . GTT G 181.28 . AC=26;AF=0.481;AN=54;BaseQRankSum=1.078;DP=79;FS=0.000;HRun=2;HaplotypeScore=43.9645;InbreedingCoeff=0.2483;MQ=29.00;MQ0=0;MQRankSum=1.524;QD=4.90;ReadPosRankSum=-0.049;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1219508 . GTGA G 294.79 . AC=34;AF=0.586;AN=58;BaseQRankSum=0.768;DP=70;FS=4.559;HRun=0;HaplotypeScore=41.3138;InbreedingCoeff=0.0804;MQ=29.00;MQ0=0;MQRankSum=2.030;QD=5.67;ReadPosRankSum=0.867;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1219522 . GTC G 520.05 . AC=42;AF=0.750;AN=56;BaseQRankSum=-1.486;DP=62;FS=0.000;HRun=0;HaplotypeScore=40.8056;InbreedingCoeff=0.1654;MQ=29.00;MQ0=0;MQRankSum=0.412;QD=10.61;ReadPosRankSum=2.095;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1219534 . GTT G 272.36 . AC=31;AF=0.534;AN=58;BaseQRankSum=0.856;DP=61;FS=0.000;HRun=2;HaplotypeScore=38.8244;InbreedingCoeff=0.3057;MQ=29.00;MQ0=0;MQRankSum=1.124;QD=6.64;ReadPosRankSum=0.386;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1219556 . GTT G 904.28 . AC=38;AF=0.826;AN=46;BaseQRankSum=2.314;DP=45;FS=0.000;HRun=2;HaplotypeScore=34.7120;InbreedingCoeff=0.2326;MQ=29.00;MQ0=0;MQRankSum=-0.104;QD=23.80;ReadPosRankSum=0.656;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1219565 . GTT G 199.22 . AC=18;AF=0.450;AN=40;BaseQRankSum=1.357;DP=40;FS=3.680;HRun=2;HaplotypeScore=38.8961;InbreedingCoeff=0.2109;MQ=29.00;MQ0=0;MQRankSum=-0.222;QD=7.38;ReadPosRankSum=-1.209;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000453732|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000453732|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1220765 . C T 169.99 . AC=1;AF=0.011;AN=92;BaseQRankSum=0.603;DP=202;Dels=0.00;FS=9.170;HRun=0;HaplotypeScore=0.1511;InbreedingCoeff=-0.0821;MQ=58.15;MQ0=0;MQRankSum=1.246;QD=18.89;ReadPosRankSum=0.238;EFF=DOWNSTREAM(MODIFIER|||||RP5-902P8.10|processed_transcript|NON_CODING|ENST00000434139|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000230415|RP5-902P8.10|ENST00000434139|||||lincRNA|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1220822 . G A 282.41 . AC=1;AF=0.0096;AN=104;BaseQRankSum=-0.135;DP=459;Dels=0.00;FS=7.514;HRun=0;HaplotypeScore=0.1948;InbreedingCoeff=-0.0472;MQ=57.39;MQ0=0;MQRankSum=-0.080;QD=14.12;ReadPosRankSum=0.116;EFF=INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|6),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|2),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|5),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),INTRON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|3);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,intron_variant&nc_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1220954 . G A 9088.59 . AC=17;AF=0.1574;AN=108;BaseQRankSum=-2.368;DP=1910;Dels=0.00;FS=13.820;HRun=0;HaplotypeScore=1.1187;InbreedingCoeff=0.4214;MQ=58.61;MQ0=0;MQRankSum=-0.837;QD=20.20;ReadPosRankSum=-1.798;EFF=EXON(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|4),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A24T|669|SCNN1D|protein_coding|CODING|ENST00000379110|),SYNONYMOUS_CODING(LOW|SILENT|tcG/tcA|S156|802|SCNN1D|protein_coding|CODING|ENST00000379116|),SYNONYMOUS_CODING(LOW|SILENT|tcG/tcA|S58|704|SCNN1D|protein_coding|CODING|ENST00000325425|);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,non_coding_exon_variant&nc_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000467651|4/4||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,synonymous_variant|tcG/tcA|S|ENSG00000162572|SCNN1D|ENST00000325425|3/15|||58/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,synonymous_variant|tcG/tcA|S|ENSG00000162572|SCNN1D|ENST00000379116|6/18|||156/802|protein_coding|1 -chr1 1221001 . A G 1982.19 . AC=3;AF=0.0273;AN=110;BaseQRankSum=-1.350;DP=1904;Dels=0.00;FS=4.488;HRun=1;HaplotypeScore=1.2454;InbreedingCoeff=0.3709;MQ=58.84;MQ0=0;MQRankSum=-1.238;QD=25.09;ReadPosRankSum=0.484;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cAg/cGg|Q172R|802|SCNN1D|protein_coding|CODING|ENST00000379116|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cAg/cGg|Q74R|704|SCNN1D|protein_coding|CODING|ENST00000325425|),SYNONYMOUS_CODING(LOW|SILENT|gcA/gcG|A39|669|SCNN1D|protein_coding|CODING|ENST00000379110|);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,missense_variant|cAg/cGg|Q/R|ENSG00000162572|SCNN1D|ENST00000325425|3/15|benign(0.108)|deleterious(0.04)|74/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,missense_variant|cAg/cGg|Q/R|ENSG00000162572|SCNN1D|ENST00000379116|6/18|benign(0.108)|deleterious(0.01)|172/802|protein_coding|1 -chr1 1221018 . C T 150.26 . AC=1;AF=0.0088;AN=114;BaseQRankSum=1.476;DP=1708;Dels=0.00;FS=1.441;HRun=0;HaplotypeScore=0.9012;InbreedingCoeff=-0.0297;MQ=58.90;MQ0=0;MQRankSum=0.438;QD=6.83;ReadPosRankSum=2.382;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cac/Tac|H178Y|802|SCNN1D|protein_coding|CODING|ENST00000379116|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cac/Tac|H80Y|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCa/gTa|A45V|669|SCNN1D|protein_coding|CODING|ENST00000379110|);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,missense_variant|Cac/Tac|H/Y|ENSG00000162572|SCNN1D|ENST00000325425|3/15|benign(0.022)|tolerated(0.53)|80/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,missense_variant|Cac/Tac|H/Y|ENSG00000162572|SCNN1D|ENST00000379116|6/18|benign(0.022)|tolerated(0.15)|178/802|protein_coding|1 -chr1 1221054 . G T 326.69 . AC=1;AF=0.0086;AN=116;BaseQRankSum=-1.105;DP=1276;Dels=0.00;FS=1.144;HRun=1;HaplotypeScore=0.9866;InbreedingCoeff=-0.0243;MQ=58.76;MQ0=0;MQRankSum=-1.148;QD=13.07;ReadPosRankSum=0.107;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|7),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|3),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|6),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1221055 . A G 407.9 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-2.031;DP=1272;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.9531;InbreedingCoeff=-0.0269;MQ=58.74;MQ0=0;MQRankSum=-1.920;QD=14.57;ReadPosRankSum=-0.545;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|7),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|3),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|6),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1221138 . G A 2422.14 . AC=12;AF=0.1000;AN=120;BaseQRankSum=1.373;DP=714;Dels=0.00;FS=4.157;HRun=0;HaplotypeScore=0.6978;InbreedingCoeff=0.2209;MQ=58.20;MQ0=0;MQRankSum=0.824;QD=19.38;ReadPosRankSum=-0.027;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|7),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|3),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|6),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1221164 . T C 91.02 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.693;DP=688;Dels=0.00;FS=9.028;HRun=0;HaplotypeScore=0.7707;InbreedingCoeff=-0.0208;MQ=57.89;MQ0=0;MQRankSum=0.498;QD=11.38;ReadPosRankSum=-0.855;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|7),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|3),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|6),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1221276 . C T 104.85 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-1.022;DP=641;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.3777;InbreedingCoeff=-0.0348;MQ=57.84;MQ0=0;MQRankSum=1.773;QD=6.55;ReadPosRankSum=0.299;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|7),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|3),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|6),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1221302 . A G 519.74 . AC=2;AF=0.0167;AN=120;BaseQRankSum=1.538;DP=606;Dels=0.00;FS=12.594;HRun=0;HaplotypeScore=0.3788;InbreedingCoeff=-0.0395;MQ=57.80;MQ0=0;MQRankSum=0.895;QD=14.85;ReadPosRankSum=-0.140;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|3),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|2),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|7),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|3),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|6),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|5);CSQ=splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1221488 . CAA C 470.7 . AC=1;AF=0.0083;AN=120;BaseQRankSum=3.941;DP=1231;FS=0.000;HRun=2;HaplotypeScore=83.4816;InbreedingCoeff=-0.0140;MQ=58.74;MQ0=0;MQRankSum=-0.574;QD=21.40;ReadPosRankSum=1.386;EFF=DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),EXON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|6),FRAME_SHIFT(HIGH||-|-115|669|SCNN1D|protein_coding|CODING|ENST00000379110|9),FRAME_SHIFT(HIGH||-|-120|138|SCNN1D|protein_coding|CODING|ENST00000470022|4),FRAME_SHIFT(HIGH||-|-150|704|SCNN1D|protein_coding|CODING|ENST00000325425|4),FRAME_SHIFT(HIGH||-|-248|802|SCNN1D|protein_coding|CODING|ENST00000379116|7),FRAME_SHIFT(HIGH||-|-84|638|SCNN1D|protein_coding|CODING|ENST00000338555|4),FRAME_SHIFT(HIGH||-|-84|638|SCNN1D|protein_coding|CODING|ENST00000400928|3);CSQ=frameshift_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555|4/15|||84/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,frameshift_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101|6/17|||216-217/328|nonsense_mediated_decay|1,frameshift_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425|4/15|||150/704|protein_coding|1,frameshift_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000470022|4/4|||120/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,frameshift_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928|3/14|||84/638|protein_coding|1,frameshift_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116|7/18|||248/802|protein_coding|1 -chr1 1221717 . C T 1847.63 . AC=12;AF=0.1053;AN=114;BaseQRankSum=0.132;DP=473;Dels=0.00;FS=1.107;HRun=0;HaplotypeScore=0.2958;InbreedingCoeff=0.1362;MQ=58.52;MQ0=0;MQRankSum=-0.162;QD=17.77;ReadPosRankSum=1.073;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|4),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|3),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|9),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|4),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|7),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|6);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1221728 . G A 60.11 . AC=1;AF=0.0089;AN=112;BaseQRankSum=-0.547;DP=393;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.3050;InbreedingCoeff=-0.0498;MQ=58.52;MQ0=0;MQRankSum=0.131;QD=15.03;ReadPosRankSum=0.389;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|4),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|3),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|9),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|4),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|7),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|6);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1222051 . A G 730.01 . AC=3;AF=0.0259;AN=116;BaseQRankSum=-1.656;DP=566;Dels=0.00;FS=1.481;HRun=4;HaplotypeScore=0.4452;InbreedingCoeff=0.4132;MQ=57.13;MQ0=0;MQRankSum=0.440;QD=29.20;ReadPosRankSum=-0.248;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|4),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|3),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|9),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|4),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|7),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|6);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1222267 . G C 6009.85 . AC=12;AF=0.1000;AN=120;BaseQRankSum=-0.365;DP=2056;Dels=0.00;FS=2.228;HRun=5;HaplotypeScore=2.3088;InbreedingCoeff=0.2341;MQ=56.92;MQ0=0;MQRankSum=1.028;QD=15.90;ReadPosRankSum=2.427;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),EXON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|7),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGc/cCc|R180P|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGc/cCc|R180P|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGc/cCc|R211P|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGc/cCc|R246P|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cGc/cCc|R344P|802|SCNN1D|protein_coding|CODING|ENST00000379116|);CSQ=missense_variant|cGc/cCc|R/P|ENSG00000162572|SCNN1D|ENST00000338555|5/15|possibly_damaging(0.646)|tolerated(0.29)|180/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,missense_variant&NMD_transcript_variant|Gcc/Ccc|A/P|ENSG00000162572|SCNN1D|ENST00000379101|7/17|unknown(0)||313/328|nonsense_mediated_decay|1,missense_variant|cGc/cCc|R/P|ENSG00000162572|SCNN1D|ENST00000325425|5/15|possibly_damaging(0.659)|tolerated(0.22)|246/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,missense_variant|cGc/cCc|R/P|ENSG00000162572|SCNN1D|ENST00000400928|4/14|possibly_damaging(0.646)|tolerated(0.29)|180/638|protein_coding|1,missense_variant|cGc/cCc|R/P|ENSG00000162572|SCNN1D|ENST00000379116|8/18|possibly_damaging(0.659)|tolerated(0.1)|344/802|protein_coding|1 -chr1 1222407 . C T 341.16 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.879;DP=1311;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=1.0043;InbreedingCoeff=-0.0091;MQ=58.24;MQ0=0;MQRankSum=1.305;QD=17.96;ReadPosRankSum=0.457;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|5),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|4),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|10),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|5),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|8),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|7);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,upstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1222598 . C T 248.87 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.379;DP=1688;Dels=0.00;FS=2.854;HRun=0;HaplotypeScore=1.2495;InbreedingCoeff=-0.0104;MQ=58.05;MQ0=0;MQRankSum=0.950;QD=9.95;ReadPosRankSum=0.137;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCg/gTg|A246V|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCg/gTg|A246V|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCg/gTg|A277V|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCg/gTg|A312V|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|gCg/gTg|A410V|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|8);CSQ=missense_variant|gCg/gTg|A/V|ENSG00000162572|SCNN1D|ENST00000338555|6/15|benign(0.005)|tolerated(0.29)|246/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|8/17|||-/328|nonsense_mediated_decay|1,missense_variant|gCg/gTg|A/V|ENSG00000162572|SCNN1D|ENST00000325425|6/15|benign(0.003)|tolerated(0.29)|312/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,missense_variant|Cgg/Tgg|R/W|ENSG00000162572|SCNN1D|ENST00000379099|1/7|unknown(0)|tolerated(0.05)|38/308|protein_coding|1,missense_variant|gCg/gTg|A/V|ENSG00000162572|SCNN1D|ENST00000400928|5/14|benign(0.005)|tolerated(0.29)|246/638|protein_coding|1,missense_variant|gCg/gTg|A/V|ENSG00000162572|SCNN1D|ENST00000379116|9/18|benign(0.003)|tolerated(0.28)|410/802|protein_coding|1 -chr1 1222690 . C T 972.9 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-2.953;DP=1085;Dels=0.00;FS=4.145;HRun=0;HaplotypeScore=2.3729;InbreedingCoeff=-0.0488;MQ=53.44;MQ0=0;MQRankSum=5.040;QD=11.58;ReadPosRankSum=-1.089;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|6),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|5),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|11),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|6),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|9),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|8);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1222695 . G C 2613.18 . AC=15;AF=0.1250;AN=120;BaseQRankSum=-2.534;DP=1083;Dels=0.00;FS=12.740;HRun=1;HaplotypeScore=2.6047;InbreedingCoeff=0.2827;MQ=53.52;MQ0=0;MQRankSum=0.965;QD=13.75;ReadPosRankSum=2.874;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|6),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|5),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|11),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|6),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|9),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|8);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1222739 . C G 156.37 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.552;DP=1353;Dels=0.00;FS=1.709;HRun=0;HaplotypeScore=1.1586;InbreedingCoeff=-0.0117;MQ=56.35;MQ0=0;MQRankSum=-0.469;QD=9.20;ReadPosRankSum=0.855;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|6),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|5),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|11),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|6),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|9),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|8);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1222842 . C T 701.62 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-4.452;DP=3950;Dels=0.00;FS=3.995;HRun=0;HaplotypeScore=2.7986;InbreedingCoeff=-0.0085;MQ=57.76;MQ0=0;MQRankSum=2.038;QD=6.05;ReadPosRankSum=-0.162;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|6),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|5),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|11),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|6),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|9),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|8);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1222958 . C T 840.29 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.301;DP=3509;DS;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=2.2081;InbreedingCoeff=-0.0084;MQ=58.04;MQ0=0;MQRankSum=0.486;QD=11.20;ReadPosRankSum=-3.869;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgc/Tgc|R297C|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgc/Tgc|R297C|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgc/Tgc|R328C|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgc/Tgc|R363C|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Cgc/Tgc|R461C|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|9);CSQ=missense_variant|Cgc/Tgc|R/C|ENSG00000162572|SCNN1D|ENST00000338555|7/15|benign(0.037)|tolerated(0.18)|297/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|9/17|||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,missense_variant|Cgc/Tgc|R/C|ENSG00000162572|SCNN1D|ENST00000325425|7/15|benign(0.075)|tolerated(0.15)|363/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,synonymous_variant|agC/agT|S|ENSG00000162572|SCNN1D|ENST00000379099|2/7|||88/308|protein_coding|1,missense_variant|Cgc/Tgc|R/C|ENSG00000162572|SCNN1D|ENST00000400928|6/14|benign(0.037)|tolerated(0.18)|297/638|protein_coding|1,missense_variant|Cgc/Tgc|R/C|ENSG00000162572|SCNN1D|ENST00000379116|10/18|benign(0.075)|tolerated(0.05)|461/802|protein_coding|1 -chr1 1222963 . C T 1912.86 . AC=2;AF=0.0167;AN=120;BaseQRankSum=0.048;DP=3402;DS;Dels=0.00;FS=5.465;HRun=0;HaplotypeScore=2.6679;InbreedingCoeff=-0.0170;MQ=58.00;MQ0=0;MQRankSum=-1.133;QD=11.45;ReadPosRankSum=1.587;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P298|638|SCNN1D|protein_coding|CODING|ENST00000338555|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P298|638|SCNN1D|protein_coding|CODING|ENST00000400928|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P329|669|SCNN1D|protein_coding|CODING|ENST00000379110|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P364|704|SCNN1D|protein_coding|CODING|ENST00000325425|),SYNONYMOUS_CODING(LOW|SILENT|ccC/ccT|P462|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|9);CSQ=synonymous_variant|ccC/ccT|P|ENSG00000162572|SCNN1D|ENST00000338555|7/15|||298/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|9/17|||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,synonymous_variant|ccC/ccT|P|ENSG00000162572|SCNN1D|ENST00000325425|7/15|||364/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,missense_variant|cCg/cTg|P/L|ENSG00000162572|SCNN1D|ENST00000379099|2/7|benign(0.04)|deleterious(0)|90/308|protein_coding|1,synonymous_variant|ccC/ccT|P|ENSG00000162572|SCNN1D|ENST00000400928|6/14|||298/638|protein_coding|1,synonymous_variant|ccC/ccT|P|ENSG00000162572|SCNN1D|ENST00000379116|10/18|||462/802|protein_coding|1 -chr1 1223385 . G C 1473.29 . AC=14;AF=0.1250;AN=112;BaseQRankSum=0.369;DP=567;Dels=0.00;FS=6.998;HRun=0;HaplotypeScore=0.6667;InbreedingCoeff=0.0907;MQ=56.62;MQ0=0;MQRankSum=0.356;QD=13.15;ReadPosRankSum=0.501;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gag/Cag|E380Q|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gag/Cag|E380Q|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gag/Cag|E411Q|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gag/Cag|E446Q|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gag/Cag|E544Q|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=missense_variant|Gag/Cag|E/Q|ENSG00000162572|SCNN1D|ENST00000338555|9/15|benign(0.006)|tolerated(0.57)|380/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|11/17|||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,missense_variant|Gag/Cag|E/Q|ENSG00000162572|SCNN1D|ENST00000325425|9/15|benign(0.012)|tolerated(0.54)|446/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,missense_variant|Gag/Cag|E/Q|ENSG00000162572|SCNN1D|ENST00000379099|3/7|benign(0.047)|tolerated(0.87)|197/308|protein_coding|1,missense_variant|Gag/Cag|E/Q|ENSG00000162572|SCNN1D|ENST00000400928|8/14|benign(0.006)|tolerated(0.57)|380/638|protein_coding|1,missense_variant|Gag/Cag|E/Q|ENSG00000162572|SCNN1D|ENST00000379116|12/18|benign(0.012)|tolerated(0.47)|544/802|protein_coding|1 -chr1 1223465 . GC G 883.64 . AC=11;AF=0.110;AN=100;BaseQRankSum=3.043;DP=292;FS=1.960;HRun=2;HaplotypeScore=26.6146;InbreedingCoeff=0.0959;MQ=54.67;MQ0=0;MQRankSum=1.559;QD=15.24;ReadPosRankSum=1.688;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223570 . C T 69.93 . AC=6;AF=0.065;AN=92;BaseQRankSum=0.235;DP=128;Dels=0.00;FS=2.433;HRun=0;HaplotypeScore=0.0933;InbreedingCoeff=0.2052;MQ=53.21;MQ0=0;MQRankSum=0.920;QD=17.48;ReadPosRankSum=-0.706;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223572 . C T 52.77 . AC=4;AF=0.044;AN=90;BaseQRankSum=1.225;DP=128;Dels=0.00;FS=2.461;HRun=0;HaplotypeScore=0.0513;InbreedingCoeff=0.1293;MQ=53.41;MQ0=0;MQRankSum=1.609;QD=17.59;ReadPosRankSum=-0.758;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223584 . T C 172.88 . AC=12;AF=0.128;AN=94;BaseQRankSum=-0.765;DP=109;Dels=0.00;FS=5.887;HRun=3;HaplotypeScore=0.0818;InbreedingCoeff=0.1717;MQ=52.94;MQ0=0;MQRankSum=-1.734;QD=15.72;ReadPosRankSum=0.356;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223608 . T C 97.92 . AC=5;AF=0.060;AN=84;BaseQRankSum=0.588;DP=98;Dels=0.00;FS=1.905;HRun=1;HaplotypeScore=0.1871;InbreedingCoeff=0.0757;MQ=49.92;MQ0=0;MQRankSum=0.349;QD=13.99;ReadPosRankSum=1.013;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223626 . T C 50.01 . AC=6;AF=0.081;AN=74;BaseQRankSum=-0.295;DP=80;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.4822;InbreedingCoeff=0.0222;MQ=49.62;MQ0=0;MQRankSum=0.318;QD=7.14;ReadPosRankSum=-0.200;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223660 . A T 124.16 . AC=11;AF=0.183;AN=60;BaseQRankSum=-2.177;DP=70;Dels=0.00;FS=6.078;HRun=0;HaplotypeScore=0.4653;InbreedingCoeff=0.0992;MQ=47.05;MQ0=0;MQRankSum=-3.819;QD=6.21;ReadPosRankSum=-0.646;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223669 . T C 30.84 . AC=2;AF=0.037;AN=54;BaseQRankSum=-0.298;DP=66;Dels=0.00;FS=2.157;HRun=2;HaplotypeScore=0.3049;InbreedingCoeff=-0.1912;MQ=46.88;MQ0=0;MQRankSum=-2.343;QD=3.85;ReadPosRankSum=-1.235;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223721 . C T 44.82 . AC=6;AF=0.150;AN=40;BaseQRankSum=0.689;DP=40;Dels=0.00;FS=2.004;HRun=0;HaplotypeScore=0.3378;InbreedingCoeff=0.0355;MQ=46.71;MQ0=0;MQRankSum=-1.020;QD=4.07;ReadPosRankSum=-1.793;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223741 . T C 53.75 . AC=4;AF=0.105;AN=38;BaseQRankSum=-0.303;DP=42;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.3143;InbreedingCoeff=-0.0438;MQ=43.53;MQ0=0;MQRankSum=1.186;QD=4.48;ReadPosRankSum=-0.745;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223754 . C T 577.41 . AC=26;AF=0.619;AN=42;BaseQRankSum=1.117;DP=42;Dels=0.00;FS=5.016;HRun=0;HaplotypeScore=0.4750;InbreedingCoeff=-0.0583;MQ=42.10;MQ0=0;MQRankSum=1.817;QD=16.04;ReadPosRankSum=-0.617;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223759 . T C 283.09 . AC=16;AF=0.400;AN=40;BaseQRankSum=0.405;DP=42;Dels=0.00;FS=20.948;HRun=2;HaplotypeScore=0.3988;InbreedingCoeff=0.0952;MQ=42.43;MQ0=0;MQRankSum=2.503;QD=12.31;ReadPosRankSum=-1.841;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223772 . C T 118.73 . AC=8;AF=0.235;AN=34;BaseQRankSum=0.790;DP=42;Dels=0.02;FS=6.468;HRun=0;HaplotypeScore=0.2464;InbreedingCoeff=-0.0704;MQ=39.82;MQ0=0;MQRankSum=-1.874;QD=6.25;ReadPosRankSum=0.384;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223790 . C T 300.88 . AC=13;AF=0.433;AN=30;BaseQRankSum=1.925;DP=47;Dels=0.04;FS=9.204;HRun=0;HaplotypeScore=0.6820;InbreedingCoeff=-0.0492;MQ=39.36;MQ0=0;MQRankSum=-0.298;QD=10.38;ReadPosRankSum=-3.275;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223795 . T C 177.98 . AC=11;AF=0.324;AN=34;BaseQRankSum=1.748;DP=49;Dels=0.00;FS=11.458;HRun=2;HaplotypeScore=1.0405;InbreedingCoeff=0.1689;MQ=39.01;MQ0=0;MQRankSum=0.452;QD=10.47;ReadPosRankSum=-2.573;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223808 . C T 254.07 . AC=14;AF=0.412;AN=34;BaseQRankSum=0.313;DP=55;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.8609;InbreedingCoeff=-0.0598;MQ=39.41;MQ0=0;MQRankSum=-2.458;QD=7.70;ReadPosRankSum=0.432;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223826 . T C 75.31 . AC=9;AF=0.188;AN=48;BaseQRankSum=-1.209;DP=64;Dels=0.00;FS=1.930;HRun=1;HaplotypeScore=0.5668;InbreedingCoeff=0.0706;MQ=39.48;MQ0=0;MQRankSum=-1.000;QD=4.71;ReadPosRankSum=0.045;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223831 . T C 93.99 . AC=8;AF=0.174;AN=46;BaseQRankSum=0.839;DP=67;Dels=0.00;FS=1.794;HRun=2;HaplotypeScore=0.6496;InbreedingCoeff=0.1389;MQ=39.56;MQ0=0;MQRankSum=-1.754;QD=7.23;ReadPosRankSum=-1.754;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223846 . T C 55.02 . AC=8;AF=0.143;AN=56;BaseQRankSum=2.634;DP=80;Dels=0.00;FS=6.864;HRun=1;HaplotypeScore=0.8057;InbreedingCoeff=-0.0215;MQ=39.58;MQ0=0;MQRankSum=-2.072;QD=2.50;ReadPosRankSum=-0.901;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223851 . T C 109.95 . AC=9;AF=0.161;AN=56;BaseQRankSum=-1.835;DP=80;Dels=0.00;FS=1.589;HRun=2;HaplotypeScore=0.5852;InbreedingCoeff=-0.0310;MQ=40.14;MQ0=0;MQRankSum=-1.699;QD=3.55;ReadPosRankSum=1.584;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223864 . T C 42.18 . AC=6;AF=0.094;AN=64;BaseQRankSum=-0.935;DP=85;Dels=0.00;FS=8.168;HRun=1;HaplotypeScore=0.4761;InbreedingCoeff=-0.1023;MQ=39.89;MQ0=0;MQRankSum=-1.619;QD=3.52;ReadPosRankSum=0.274;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223873 . T TCC 58.95 . AC=5;AF=0.076;AN=66;BaseQRankSum=2.703;DP=91;FS=1.912;HRun=3;HaplotypeScore=29.9562;InbreedingCoeff=-0.1751;MQ=40.59;MQ0=0;MQRankSum=-2.282;QD=3.27;ReadPosRankSum=-0.842;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223887 . C T 208.26 . AC=15;AF=0.242;AN=62;BaseQRankSum=1.248;DP=86;Dels=0.00;FS=1.200;HRun=0;HaplotypeScore=0.4017;InbreedingCoeff=-0.1357;MQ=40.83;MQ0=0;MQRankSum=-2.152;QD=4.84;ReadPosRankSum=0.390;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223900 . C T 905.84 . AC=36;AF=0.643;AN=56;BaseQRankSum=0.938;DP=77;Dels=0.00;FS=0.998;HRun=0;HaplotypeScore=0.6461;InbreedingCoeff=0.0959;MQ=40.99;MQ0=0;MQRankSum=-1.278;QD=14.38;ReadPosRankSum=0.258;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223905 . T C 98.29 . AC=9;AF=0.167;AN=54;BaseQRankSum=-1.946;DP=77;Dels=0.00;FS=3.277;HRun=2;HaplotypeScore=0.5934;InbreedingCoeff=0.1031;MQ=40.55;MQ0=0;MQRankSum=-0.402;QD=5.46;ReadPosRankSum=-2.098;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1223963 . T C 128.73 . AC=10;AF=0.385;AN=26;BaseQRankSum=-0.635;DP=29;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.4996;InbreedingCoeff=-0.0973;MQ=39.99;MQ0=0;MQRankSum=1.102;QD=6.78;ReadPosRankSum=0.301;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225096 . C T 123.66 . AC=9;AF=0.563;AN=16;BaseQRankSum=0.278;DP=16;Dels=0.00;FS=6.990;HRun=0;HaplotypeScore=0.8108;MQ=33.81;MQ0=0;MQRankSum=-1.246;QD=13.74;ReadPosRankSum=1.769;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225121 . T C 79.61 . AC=9;AF=0.300;AN=30;BaseQRankSum=-0.698;DP=26;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=1.1615;InbreedingCoeff=0.2471;MQ=30.87;MQ0=0;MQRankSum=-1.397;QD=11.37;ReadPosRankSum=-0.698;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225125 . TCC T 136.07 . AC=9;AF=0.281;AN=32;BaseQRankSum=0.701;DP=25;FS=18.560;HRun=5;HaplotypeScore=19.9646;InbreedingCoeff=-0.0780;MQ=30.60;MQ0=0;MQRankSum=2.186;QD=11.34;ReadPosRankSum=1.196;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225141 . T C 32.44 . AC=5;AF=0.147;AN=34;BaseQRankSum=-0.213;DP=26;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.3484;InbreedingCoeff=0.0778;MQ=30.54;MQ0=0;MQRankSum=-1.319;QD=8.11;ReadPosRankSum=-1.149;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225163 . TCC T 121.19 . AC=12;AF=0.353;AN=34;BaseQRankSum=0.452;DP=22;FS=9.260;HRun=5;HaplotypeScore=17.1552;InbreedingCoeff=0.3248;MQ=32.45;MQ0=0;MQRankSum=-1.759;QD=17.31;ReadPosRankSum=0.050;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225174 . C T 237.41 . AC=18;AF=0.643;AN=28;BaseQRankSum=0.648;DP=22;Dels=0.00;FS=2.363;HRun=0;HaplotypeScore=1.0277;InbreedingCoeff=0.3305;MQ=32.25;MQ0=0;MQRankSum=-0.648;QD=26.38;ReadPosRankSum=-0.059;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225239 . A T 100.39 . AC=6;AF=0.500;AN=12;BaseQRankSum=0.550;DP=8;Dels=0.00;FS=3.680;HRun=0;HaplotypeScore=0.0000;MQ=42.45;MQ0=0;MQRankSum=0.550;QD=25.10;ReadPosRankSum=0.550;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225261 . T C 133.06 . AC=10;AF=0.200;AN=50;BaseQRankSum=0.107;DP=45;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.3102;InbreedingCoeff=0.1724;MQ=37.78;MQ0=0;MQRankSum=-0.997;QD=14.78;ReadPosRankSum=-0.534;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225279 . T C 111.05 . AC=13;AF=0.210;AN=62;BaseQRankSum=0.114;DP=66;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.5124;InbreedingCoeff=0.2853;MQ=35.30;MQ0=0;MQRankSum=-1.427;QD=11.10;ReadPosRankSum=-1.376;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225284 . T C 256.36 . AC=13;AF=0.210;AN=62;BaseQRankSum=0.970;DP=68;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=0.5134;InbreedingCoeff=0.1617;MQ=35.13;MQ0=0;MQRankSum=-1.184;QD=12.82;ReadPosRankSum=0.233;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225302 . C T 193.86 . AC=15;AF=0.242;AN=62;BaseQRankSum=-1.436;DP=91;Dels=0.00;FS=1.834;HRun=0;HaplotypeScore=1.1816;InbreedingCoeff=0.2195;MQ=33.76;MQ0=0;MQRankSum=0.576;QD=8.43;ReadPosRankSum=1.178;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225312 . G A 158.46 . AC=11;AF=0.172;AN=64;BaseQRankSum=-0.966;DP=93;Dels=0.02;FS=7.390;HRun=0;HaplotypeScore=1.6923;InbreedingCoeff=-0.0065;MQ=33.88;MQ0=0;MQRankSum=-1.247;QD=6.09;ReadPosRankSum=0.910;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225319 . T C 311.15 . AC=16;AF=0.229;AN=70;BaseQRankSum=0.007;DP=97;Dels=0.00;FS=12.678;HRun=1;HaplotypeScore=0.6200;InbreedingCoeff=0.0685;MQ=35.32;MQ0=0;MQRankSum=-2.217;QD=9.15;ReadPosRankSum=2.287;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225325 . G A 205.95 . AC=12;AF=0.176;AN=68;BaseQRankSum=-2.064;DP=103;Dels=0.00;FS=4.701;HRun=0;HaplotypeScore=0.5398;InbreedingCoeff=-0.0183;MQ=36.25;MQ0=0;MQRankSum=-2.585;QD=6.86;ReadPosRankSum=1.419;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225330 . G A 109.49 . AC=8;AF=0.129;AN=62;BaseQRankSum=-2.003;DP=109;Dels=0.00;FS=4.693;HRun=0;HaplotypeScore=0.7228;InbreedingCoeff=-0.1427;MQ=36.47;MQ0=0;MQRankSum=-2.668;QD=3.78;ReadPosRankSum=0.963;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225335 . T C 238.86 . AC=13;AF=0.191;AN=68;BaseQRankSum=-1.449;DP=110;Dels=0.00;FS=7.186;HRun=1;HaplotypeScore=0.5462;InbreedingCoeff=-0.0481;MQ=36.64;MQ0=0;MQRankSum=-2.715;QD=6.46;ReadPosRankSum=-1.710;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225340 . TC T 227.19 . AC=9;AF=0.132;AN=68;BaseQRankSum=5.045;DP=112;FS=7.004;HRun=2;HaplotypeScore=30.7520;InbreedingCoeff=-0.0950;MQ=37.06;MQ0=0;MQRankSum=-2.051;QD=7.33;ReadPosRankSum=-0.410;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225343 . G A 137.99 . AC=9;AF=0.141;AN=64;BaseQRankSum=-2.584;DP=116;Dels=0.01;FS=2.292;HRun=0;HaplotypeScore=0.6542;InbreedingCoeff=-0.1498;MQ=37.81;MQ0=0;MQRankSum=-2.776;QD=4.45;ReadPosRankSum=-2.598;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225350 . G C 61.74 . AC=4;AF=0.065;AN=62;BaseQRankSum=-1.443;DP=105;Dels=0.00;FS=2.393;HRun=5;HaplotypeScore=0.7473;InbreedingCoeff=-0.1912;MQ=39.32;MQ0=0;MQRankSum=-2.240;QD=4.75;ReadPosRankSum=-3.424;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225371 . C T 52.17 . AC=8;AF=0.114;AN=70;BaseQRankSum=0.850;DP=87;Dels=0.00;FS=2.411;HRun=0;HaplotypeScore=0.1882;InbreedingCoeff=0.0721;MQ=42.92;MQ0=0;MQRankSum=-1.185;QD=3.07;ReadPosRankSum=-0.451;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225389 . C T 253.01 . AC=13;AF=0.167;AN=78;BaseQRankSum=1.797;DP=127;Dels=0.00;FS=3.410;HRun=0;HaplotypeScore=0.3513;InbreedingCoeff=0.1116;MQ=41.93;MQ0=0;MQRankSum=-1.326;QD=9.04;ReadPosRankSum=-2.083;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225407 . T C 47.15 . AC=5;AF=0.054;AN=92;BaseQRankSum=-1.004;DP=162;Dels=0.00;FS=2.276;HRun=1;HaplotypeScore=0.5729;InbreedingCoeff=-0.1734;MQ=42.85;MQ0=0;MQRankSum=-1.615;QD=1.68;ReadPosRankSum=2.014;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225425 . C T 793.83 . AC=31;AF=0.337;AN=92;BaseQRankSum=1.412;DP=187;Dels=0.02;FS=6.178;HRun=0;HaplotypeScore=0.4105;InbreedingCoeff=-0.0399;MQ=43.23;MQ0=0;MQRankSum=-4.142;QD=6.56;ReadPosRankSum=0.059;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225443 . T C 468.02 . AC=15;AF=0.163;AN=92;BaseQRankSum=-2.292;DP=235;Dels=0.00;FS=6.953;HRun=1;HaplotypeScore=0.6031;InbreedingCoeff=-0.1815;MQ=43.46;MQ0=0;MQRankSum=-0.753;QD=4.78;ReadPosRankSum=-1.745;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225452 . T TCC 330.82 . AC=10;AF=0.104;AN=96;BaseQRankSum=1.749;DP=270;FS=4.169;HRun=3;HaplotypeScore=57.3296;InbreedingCoeff=-0.1229;MQ=43.34;MQ0=0;MQRankSum=-2.041;QD=5.09;ReadPosRankSum=-2.421;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225461 . T C 238.55 . AC=14;AF=0.1273;AN=110;BaseQRankSum=-0.273;DP=319;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.9594;InbreedingCoeff=-0.1003;MQ=43.75;MQ0=0;MQRankSum=-1.815;QD=2.41;ReadPosRankSum=0.946;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225466 . T C 184.63 . AC=11;AF=0.0948;AN=116;BaseQRankSum=-1.477;DP=342;Dels=0.00;FS=1.813;HRun=2;HaplotypeScore=0.9329;InbreedingCoeff=-0.1688;MQ=44.09;MQ0=0;MQRankSum=-2.845;QD=1.85;ReadPosRankSum=-1.214;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225470 . T TCC 366.85 . AC=11;AF=0.0948;AN=116;BaseQRankSum=3.645;DP=363;FS=6.315;HRun=3;HaplotypeScore=61.2598;InbreedingCoeff=-0.1667;MQ=44.55;MQ0=0;MQRankSum=-2.973;QD=4.32;ReadPosRankSum=-1.690;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225479 . T C 216.38 . AC=8;AF=0.0690;AN=116;BaseQRankSum=-0.622;DP=392;Dels=0.00;FS=4.070;HRun=1;HaplotypeScore=1.0837;InbreedingCoeff=0.0601;MQ=45.51;MQ0=0;MQRankSum=-3.522;QD=3.73;ReadPosRankSum=-0.410;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225484 . T C 214.92 . AC=16;AF=0.1379;AN=116;BaseQRankSum=-1.446;DP=412;Dels=0.00;FS=17.524;HRun=2;HaplotypeScore=1.1210;InbreedingCoeff=-0.1039;MQ=45.67;MQ0=0;MQRankSum=-3.630;QD=1.52;ReadPosRankSum=1.200;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225488 . T TCC 73.7 . AC=6;AF=0.0517;AN=116;BaseQRankSum=3.896;DP=442;FS=2.167;HRun=3;HaplotypeScore=66.4298;InbreedingCoeff=-0.0944;MQ=46.00;MQ0=0;MQRankSum=-2.159;QD=1.23;ReadPosRankSum=-2.147;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225497 . T C 35.56 . AC=5;AF=0.0439;AN=114;BaseQRankSum=-3.665;DP=477;Dels=0.00;FS=3.756;HRun=1;HaplotypeScore=1.4250;InbreedingCoeff=-0.0704;MQ=45.97;MQ0=0;MQRankSum=-2.358;QD=0.73;ReadPosRankSum=0.893;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225502 . T C 688.93 . AC=16;AF=0.1404;AN=114;BaseQRankSum=0.243;DP=497;Dels=0.00;FS=19.856;HRun=2;HaplotypeScore=1.6402;InbreedingCoeff=-0.0754;MQ=46.34;MQ0=0;MQRankSum=-4.470;QD=5.22;ReadPosRankSum=0.940;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225504 . C T 33.71 . AC=3;AF=0.0263;AN=114;BaseQRankSum=-2.542;DP=505;Dels=0.00;FS=4.534;HRun=0;HaplotypeScore=1.6971;InbreedingCoeff=-0.0570;MQ=46.78;MQ0=0;MQRankSum=1.064;QD=1.16;ReadPosRankSum=2.168;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225505 . G A 87.65 . AC=6;AF=0.0526;AN=114;BaseQRankSum=0.061;DP=508;Dels=0.01;FS=0.000;HRun=0;HaplotypeScore=1.7900;InbreedingCoeff=-0.0837;MQ=47.10;MQ0=0;MQRankSum=-0.730;QD=1.65;ReadPosRankSum=0.134;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225506 . TCC T 120.88 . AC=8;AF=0.0702;AN=114;BaseQRankSum=7.487;DP=525;FS=3.030;HRun=5;HaplotypeScore=77.2182;InbreedingCoeff=-0.0982;MQ=47.37;MQ0=0;MQRankSum=-3.071;QD=1.92;ReadPosRankSum=-7.075;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_truncation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225507 . C T 63.16 . AC=5;AF=0.0439;AN=114;BaseQRankSum=0.195;DP=525;Dels=0.01;FS=0.000;HRun=1;HaplotypeScore=2.6528;InbreedingCoeff=-0.0729;MQ=47.37;MQ0=0;MQRankSum=-0.118;QD=1.58;ReadPosRankSum=1.001;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225512 . G A 59.61 . AC=5;AF=0.0431;AN=116;BaseQRankSum=0.671;DP=562;Dels=0.01;FS=0.000;HRun=0;HaplotypeScore=2.9722;InbreedingCoeff=-0.0588;MQ=48.31;MQ0=0;MQRankSum=-3.136;QD=1.24;ReadPosRankSum=-1.130;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225517 . T C 31.29 . AC=10;AF=0.0862;AN=116;BaseQRankSum=-0.543;DP=597;Dels=0.00;FS=8.293;HRun=1;HaplotypeScore=2.0472;InbreedingCoeff=0.0340;MQ=48.76;MQ0=0;MQRankSum=-2.154;QD=0.33;ReadPosRankSum=-1.343;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225522 . T C 265.0 . AC=14;AF=0.1207;AN=116;BaseQRankSum=-0.678;DP=629;Dels=0.00;FS=97.747;HRun=2;HaplotypeScore=1.7833;InbreedingCoeff=-0.1442;MQ=49.06;MQ0=0;MQRankSum=-5.490;QD=1.44;ReadPosRankSum=-0.496;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225526 . T TCC 411.2 . AC=10;AF=0.0862;AN=116;BaseQRankSum=4.261;DP=667;FS=14.873;HRun=3;HaplotypeScore=89.4036;InbreedingCoeff=-0.1134;MQ=49.86;MQ0=0;MQRankSum=-3.366;QD=3.29;ReadPosRankSum=-3.904;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225535 . T C 848.26 . AC=10;AF=0.0862;AN=116;BaseQRankSum=-1.442;DP=750;Dels=0.00;FS=4.302;HRun=1;HaplotypeScore=1.3486;InbreedingCoeff=-0.1129;MQ=50.86;MQ0=0;MQRankSum=1.251;QD=5.89;ReadPosRankSum=-1.457;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225540 . T C 45.1 . AC=3;AF=0.0259;AN=116;BaseQRankSum=-1.838;DP=782;Dels=0.00;FS=14.682;HRun=2;HaplotypeScore=1.0795;InbreedingCoeff=-0.0391;MQ=51.27;MQ0=0;MQRankSum=-3.072;QD=2.37;ReadPosRankSum=-2.538;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225543 . G C 72.05 . AC=1;AF=0.0086;AN=116;BaseQRankSum=1.703;DP=821;Dels=0.00;FS=0.000;HRun=2;HaplotypeScore=1.7205;InbreedingCoeff=-0.0188;MQ=51.72;MQ0=0;MQRankSum=1.023;QD=3.79;ReadPosRankSum=-1.656;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225544 . T TCC 104.04 . AC=3;AF=0.0263;AN=114;BaseQRankSum=5.030;DP=839;FS=11.849;HRun=3;HaplotypeScore=111.7887;InbreedingCoeff=-0.0353;MQ=51.88;MQ0=0;MQRankSum=-4.162;QD=3.15;ReadPosRankSum=-3.869;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225553 . C T 5273.44 . AC=32;AF=0.2807;AN=114;BaseQRankSum=1.380;DP=911;Dels=0.00;FS=0.497;HRun=0;HaplotypeScore=1.8119;InbreedingCoeff=-0.1881;MQ=52.16;MQ0=0;MQRankSum=2.230;QD=11.39;ReadPosRankSum=-0.044;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225558 . T C 207.02 . AC=1;AF=0.0086;AN=116;BaseQRankSum=0.020;DP=961;Dels=0.00;FS=8.305;HRun=2;HaplotypeScore=1.2090;InbreedingCoeff=-0.0183;MQ=52.59;MQ0=0;MQRankSum=-0.514;QD=12.94;ReadPosRankSum=1.515;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225562 . T TCC 147.67 . AC=5;AF=0.0417;AN=120;BaseQRankSum=3.446;DP=1028;FS=15.375;HRun=3;HaplotypeScore=125.4377;InbreedingCoeff=0.2845;MQ=53.39;MQ0=0;MQRankSum=-2.465;QD=3.14;ReadPosRankSum=-3.790;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225571 . C T 5357.09 . AC=38;AF=0.3167;AN=120;BaseQRankSum=-0.715;DP=1167;Dels=0.00;FS=5.301;HRun=0;HaplotypeScore=2.7736;InbreedingCoeff=0.0326;MQ=54.57;MQ0=0;MQRankSum=-3.297;QD=10.50;ReadPosRankSum=-0.766;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225576 . T C 4461.84 . AC=22;AF=0.1833;AN=120;BaseQRankSum=2.082;DP=1262;Dels=0.00;FS=21.631;HRun=2;HaplotypeScore=2.8144;InbreedingCoeff=0.1776;MQ=54.78;MQ0=0;MQRankSum=0.294;QD=13.48;ReadPosRankSum=0.090;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225579 . G C 1702.39 . AC=10;AF=0.0833;AN=120;BaseQRankSum=1.093;DP=1332;Dels=0.00;FS=11.111;HRun=2;HaplotypeScore=2.9711;InbreedingCoeff=0.0608;MQ=55.00;MQ0=0;MQRankSum=0.576;QD=11.74;ReadPosRankSum=0.329;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225597 . G A 764.88 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.619;DP=1769;Dels=0.00;FS=3.258;HRun=0;HaplotypeScore=1.9881;InbreedingCoeff=-0.0171;MQ=55.96;MQ0=0;MQRankSum=3.394;QD=15.61;ReadPosRankSum=2.984;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225604 . A G 288.23 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.165;DP=1945;Dels=0.00;FS=50.790;HRun=0;HaplotypeScore=2.6125;InbreedingCoeff=-0.0085;MQ=56.12;MQ0=0;MQRankSum=-4.687;QD=13.10;ReadPosRankSum=-3.197;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225612 . G A 8878.59 . AC=25;AF=0.2083;AN=120;BaseQRankSum=-2.436;DP=2093;Dels=0.00;FS=21.815;HRun=0;HaplotypeScore=1.7123;InbreedingCoeff=0.1407;MQ=56.18;MQ0=0;MQRankSum=1.022;QD=13.60;ReadPosRankSum=-1.768;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225641 . C A 16235.21 . AC=27;AF=0.2250;AN=120;BaseQRankSum=-0.237;DP=2629;Dels=0.00;FS=1.703;HRun=0;HaplotypeScore=1.7639;InbreedingCoeff=0.3777;MQ=56.48;MQ0=0;MQRankSum=2.792;QD=20.32;ReadPosRankSum=2.777;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|9),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|8),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|14),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|9),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|12),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|11);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225804 . A G 641.95 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.178;DP=3135;Dels=0.00;FS=4.019;HRun=0;HaplotypeScore=3.7390;InbreedingCoeff=-0.0085;MQ=57.48;MQ0=0;MQRankSum=2.112;QD=13.10;ReadPosRankSum=1.189;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|10),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|9),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|15),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|10),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|13),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|12);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225810 . C CGG 5006.7 . AC=3;AF=0.0250;AN=120;BaseQRankSum=1.861;DP=3131;FS=0.360;HRun=6;HaplotypeScore=237.1014;InbreedingCoeff=0.6361;MQ=57.43;MQ0=0;MQRankSum=-0.361;QD=33.60;ReadPosRankSum=-1.506;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|10),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|9),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|15),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|10),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|13),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|12);CSQ=intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant&feature_elongation|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225824 . G A 1475.6 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.326;DP=3390;Dels=0.00;FS=1.074;HRun=1;HaplotypeScore=2.3868;InbreedingCoeff=-0.0086;MQ=57.43;MQ0=0;MQRankSum=2.353;QD=12.20;ReadPosRankSum=-2.766;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|10),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|9),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|15),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|10),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|13),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|12);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225945 . G C 3949.71 . AC=3;AF=0.0250;AN=120;BaseQRankSum=-1.101;DP=3777;Dels=0.00;FS=22.292;HRun=0;HaplotypeScore=4.0017;InbreedingCoeff=0.6569;MQ=57.83;MQ0=0;MQRankSum=-0.665;QD=25.32;ReadPosRankSum=2.508;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|11),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|10),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|16),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|11),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|14),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|13);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1225959 . C G 25993.98 . AC=36;AF=0.3000;AN=120;BaseQRankSum=1.580;DP=3659;Dels=0.00;FS=21.530;HRun=2;HaplotypeScore=2.4662;InbreedingCoeff=0.2055;MQ=57.98;MQ0=0;MQRankSum=1.714;QD=17.82;ReadPosRankSum=0.723;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|processed_transcript|CODING|ENST00000467651|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|11),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|10),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|16),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|11),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|14),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|13);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000467651|||||processed_transcript|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1226063 . G A 1804.82 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-2.166;DP=2009;Dels=0.00;FS=1.053;HRun=0;HaplotypeScore=1.1275;InbreedingCoeff=-0.0436;MQ=58.53;MQ0=0;MQRankSum=0.440;QD=9.35;ReadPosRankSum=-0.405;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A472T|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A472T|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A503T|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A538T|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gcc/Acc|A636T|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=missense_variant|Gcc/Acc|A/T|ENSG00000162572|SCNN1D|ENST00000338555|12/15|benign(0.168)|tolerated(0.19)|472/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|14/17|||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,missense_variant|Gcc/Acc|A/T|ENSG00000162572|SCNN1D|ENST00000325425|12/15|benign(0.109)|tolerated(0.39)|538/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,missense_variant|Gcc/Acc|A/T|ENSG00000162572|SCNN1D|ENST00000379099|6/7|benign(0.297)|tolerated(0.13)|289/308|protein_coding|1,missense_variant|Gcc/Acc|A/T|ENSG00000162572|SCNN1D|ENST00000400928|11/14|benign(0.168)|tolerated(0.19)|472/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,missense_variant|Gcc/Acc|A/T|ENSG00000162572|SCNN1D|ENST00000379116|15/18|benign(0.109)|tolerated(0.29)|636/802|protein_coding|1 -chr1 1226102 . G A 4016.45 . AC=11;AF=0.0917;AN=120;BaseQRankSum=-1.245;DP=1541;Dels=0.00;FS=2.281;HRun=0;HaplotypeScore=1.2555;InbreedingCoeff=0.2951;MQ=58.35;MQ0=0;MQRankSum=0.176;QD=16.67;ReadPosRankSum=2.379;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|12),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|11),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|17),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|12),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|15),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1226139 . G A 53.13 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.435;DP=1276;Dels=0.00;FS=8.998;HRun=1;HaplotypeScore=0.7541;InbreedingCoeff=-0.0093;MQ=58.09;MQ0=0;MQRankSum=-1.197;QD=2.95;ReadPosRankSum=-0.731;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|12),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|11),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|17),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|12),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|15),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1226217 . A G 385.18 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.713;DP=1445;Dels=0.00;FS=1.054;HRun=0;HaplotypeScore=1.4065;InbreedingCoeff=-0.0114;MQ=58.08;MQ0=0;MQRankSum=0.322;QD=12.84;ReadPosRankSum=0.561;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|12),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|11),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|17),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|12),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|15),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1226221 . A G 12961.74 . AC=36;AF=0.3000;AN=120;BaseQRankSum=0.407;DP=1473;Dels=0.00;FS=8.750;HRun=2;HaplotypeScore=1.9078;InbreedingCoeff=0.3380;MQ=58.10;MQ0=0;MQRankSum=0.672;QD=20.00;ReadPosRankSum=-0.137;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|12),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|11),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|17),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|12),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|15),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1226231 . G A 1060.58 . AC=4;AF=0.0333;AN=120;BaseQRankSum=0.134;DP=1522;Dels=0.00;FS=3.076;HRun=0;HaplotypeScore=2.2801;InbreedingCoeff=-0.0375;MQ=58.05;MQ0=0;MQRankSum=1.141;QD=13.26;ReadPosRankSum=0.602;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|12),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|11),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|17),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|12),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|15),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1226233 . G A 432.75 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.014;DP=1544;Dels=0.00;FS=11.621;HRun=0;HaplotypeScore=1.7754;InbreedingCoeff=-0.0115;MQ=58.14;MQ0=0;MQRankSum=1.437;QD=9.21;ReadPosRankSum=-0.335;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|12),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|11),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|17),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|12),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|15),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1226246 . C T 658.46 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.957;DP=1671;Dels=0.00;FS=1.750;HRun=0;HaplotypeScore=1.4315;InbreedingCoeff=-0.0192;MQ=58.19;MQ0=0;MQRankSum=1.240;QD=9.98;ReadPosRankSum=-0.155;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|12),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|11),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|17),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|12),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|15),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1226267 . G A 198.26 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.326;DP=1848;Dels=0.00;FS=1.356;HRun=0;HaplotypeScore=0.8439;InbreedingCoeff=-0.0108;MQ=58.43;MQ0=0;MQRankSum=-0.958;QD=8.62;ReadPosRankSum=1.398;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|12),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|11),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|17),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|12),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|15),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|14);CSQ=splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,splice_region_variant&intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,splice_region_variant&intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1226292 . G A 2311.15 . AC=4;AF=0.0333;AN=120;BaseQRankSum=-0.197;DP=2007;Dels=0.00;FS=0.860;HRun=0;HaplotypeScore=1.1337;InbreedingCoeff=-0.0381;MQ=58.08;MQ0=0;MQRankSum=0.237;QD=11.79;ReadPosRankSum=1.765;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),SYNONYMOUS_CODING(LOW|SILENT|acG/acA|T481|638|SCNN1D|protein_coding|CODING|ENST00000338555|),SYNONYMOUS_CODING(LOW|SILENT|acG/acA|T481|638|SCNN1D|protein_coding|CODING|ENST00000400928|),SYNONYMOUS_CODING(LOW|SILENT|acG/acA|T512|669|SCNN1D|protein_coding|CODING|ENST00000379110|),SYNONYMOUS_CODING(LOW|SILENT|acG/acA|T547|704|SCNN1D|protein_coding|CODING|ENST00000325425|),SYNONYMOUS_CODING(LOW|SILENT|acG/acA|T645|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|15);CSQ=synonymous_variant|acG/acA|T|ENSG00000162572|SCNN1D|ENST00000338555|13/15|||481/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|15/17|||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,synonymous_variant|acG/acA|T|ENSG00000162572|SCNN1D|ENST00000325425|13/15|||547/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,synonymous_variant|acG/acA|T|ENSG00000162572|SCNN1D|ENST00000379099|7/7|||298/308|protein_coding|1,synonymous_variant|acG/acA|T|ENSG00000162572|SCNN1D|ENST00000400928|12/14|||481/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,synonymous_variant|acG/acA|T|ENSG00000162572|SCNN1D|ENST00000379116|16/18|||645/802|protein_coding|1 -chr1 1226348 . C G 14565.59 . AC=27;AF=0.2250;AN=120;BaseQRankSum=-4.148;DP=2185;Dels=0.00;FS=12.103;HRun=0;HaplotypeScore=3.8823;InbreedingCoeff=0.3031;MQ=57.72;MQ0=0;MQRankSum=-0.548;QD=19.76;ReadPosRankSum=2.686;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|13),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|12),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|18),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|13),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|16),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|15);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1226350 . C T 913.84 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.958;DP=2174;Dels=0.00;FS=1.375;HRun=0;HaplotypeScore=3.4324;InbreedingCoeff=-0.0112;MQ=57.70;MQ0=0;MQRankSum=0.687;QD=12.52;ReadPosRankSum=2.553;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|13),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|12),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|18),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|13),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|16),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|15);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1226418 . C T 612.71 . AC=1;AF=0.0083;AN=120;BaseQRankSum=0.522;DP=1764;Dels=0.00;FS=13.559;HRun=0;HaplotypeScore=1.3627;InbreedingCoeff=-0.0097;MQ=57.73;MQ0=0;MQRankSum=0.737;QD=13.93;ReadPosRankSum=-1.089;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|13),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|12),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|18),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|13),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|16),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|15);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1226419 . G A 122.55 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-2.320;DP=1752;Dels=0.00;FS=6.374;HRun=0;HaplotypeScore=1.2212;InbreedingCoeff=-0.0099;MQ=57.75;MQ0=0;MQRankSum=0.393;QD=5.33;ReadPosRankSum=0.165;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|13),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|12),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|18),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|13),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|16),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|15);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1226423 . T C 2109.1 . AC=4;AF=0.0333;AN=120;BaseQRankSum=1.172;DP=1776;Dels=0.00;FS=0.924;HRun=3;HaplotypeScore=1.1033;InbreedingCoeff=0.4687;MQ=57.78;MQ0=0;MQRankSum=3.304;QD=24.24;ReadPosRankSum=0.918;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|13),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|12),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|18),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|13),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|16),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|15);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1226512 . G A 620.19 . AC=1;AF=0.0083;AN=120;BaseQRankSum=1.007;DP=1562;Dels=0.00;FS=9.380;HRun=0;HaplotypeScore=0.9448;InbreedingCoeff=-0.0116;MQ=58.77;MQ0=0;MQRankSum=0.069;QD=24.81;ReadPosRankSum=-2.040;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V518M|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V518M|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V549M|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V584M|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Gtg/Atg|V682M|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|16);CSQ=missense_variant|Gtg/Atg|V/M|ENSG00000162572|SCNN1D|ENST00000338555|14/15|benign(0.109)|tolerated(0.11)|518/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|16/17|||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,missense_variant|Gtg/Atg|V/M|ENSG00000162572|SCNN1D|ENST00000325425|14/15|benign(0.036)|tolerated(0.12)|584/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,missense_variant|Gtg/Atg|V/M|ENSG00000162572|SCNN1D|ENST00000400928|13/14|benign(0.109)|tolerated(0.11)|518/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,missense_variant|Gtg/Atg|V/M|ENSG00000162572|SCNN1D|ENST00000379116|17/18|benign(0.036)|tolerated(0.1)|682/802|protein_coding|1 -chr1 1226539 . C T 817.48 . AC=5;AF=0.0417;AN=120;BaseQRankSum=-2.085;DP=1317;Dels=0.00;FS=0.000;HRun=0;HaplotypeScore=0.6593;InbreedingCoeff=-0.0489;MQ=58.71;MQ0=0;MQRankSum=0.576;QD=13.62;ReadPosRankSum=0.439;EFF=DOWNSTREAM(MODIFIER||||138|SCNN1D|protein_coding|CODING|ENST00000470022|),DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|14),INTRON(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|13),INTRON(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|19),INTRON(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|14),INTRON(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|17),INTRON(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|16);CSQ=intron_variant|||ENSG00000162572|SCNN1D|ENST00000338555||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,intron_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000325425||||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000470022||||-/139|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000400928||||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,intron_variant|||ENSG00000162572|SCNN1D|ENST00000379116||||-/802|protein_coding|1 -chr1 1226651 . G C 418.81 . AC=5;AF=0.0417;AN=120;BaseQRankSum=2.115;DP=667;Dels=0.00;FS=0.000;HRun=1;HaplotypeScore=0.3223;InbreedingCoeff=-0.0562;MQ=58.20;MQ0=0;MQRankSum=0.758;QD=9.74;ReadPosRankSum=-0.561;EFF=DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),SYNONYMOUS_CODING(LOW|SILENT|tcG/tcC|S526|638|SCNN1D|protein_coding|CODING|ENST00000338555|),SYNONYMOUS_CODING(LOW|SILENT|tcG/tcC|S526|638|SCNN1D|protein_coding|CODING|ENST00000400928|),SYNONYMOUS_CODING(LOW|SILENT|tcG/tcC|S557|669|SCNN1D|protein_coding|CODING|ENST00000379110|),SYNONYMOUS_CODING(LOW|SILENT|tcG/tcC|S592|704|SCNN1D|protein_coding|CODING|ENST00000325425|),SYNONYMOUS_CODING(LOW|SILENT|tcG/tcC|S690|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|17);CSQ=synonymous_variant|tcG/tcC|S|ENSG00000162572|SCNN1D|ENST00000338555|15/15|||526/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|17/17|||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,synonymous_variant|tcG/tcC|S|ENSG00000162572|SCNN1D|ENST00000325425|15/15|||592/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,synonymous_variant|tcG/tcC|S|ENSG00000162572|SCNN1D|ENST00000400928|14/14|||526/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,synonymous_variant|tcG/tcC|S|ENSG00000162572|SCNN1D|ENST00000379116|18/18|||690/802|protein_coding|1 -chr1 1226690 . C T 282.98 . AC=2;AF=0.0167;AN=120;BaseQRankSum=-0.735;DP=758;Dels=0.00;FS=19.978;HRun=0;HaplotypeScore=0.3775;InbreedingCoeff=-0.0239;MQ=58.31;MQ0=0;MQRankSum=0.192;QD=12.30;ReadPosRankSum=0.815;EFF=DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),SYNONYMOUS_CODING(LOW|SILENT|tcC/tcT|S539|638|SCNN1D|protein_coding|CODING|ENST00000338555|),SYNONYMOUS_CODING(LOW|SILENT|tcC/tcT|S539|638|SCNN1D|protein_coding|CODING|ENST00000400928|),SYNONYMOUS_CODING(LOW|SILENT|tcC/tcT|S570|669|SCNN1D|protein_coding|CODING|ENST00000379110|),SYNONYMOUS_CODING(LOW|SILENT|tcC/tcT|S605|704|SCNN1D|protein_coding|CODING|ENST00000325425|),SYNONYMOUS_CODING(LOW|SILENT|tcC/tcT|S703|802|SCNN1D|protein_coding|CODING|ENST00000379116|),UTR_3_PRIME(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|17);CSQ=synonymous_variant|tcC/tcT|S|ENSG00000162572|SCNN1D|ENST00000338555|15/15|||539/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,3_prime_UTR_variant&NMD_transcript_variant|||ENSG00000162572|SCNN1D|ENST00000379101|17/17|||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,synonymous_variant|tcC/tcT|S|ENSG00000162572|SCNN1D|ENST00000325425|15/15|||605/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,synonymous_variant|tcC/tcT|S|ENSG00000162572|SCNN1D|ENST00000400928|14/14|||539/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,synonymous_variant|tcC/tcT|S|ENSG00000162572|SCNN1D|ENST00000379116|18/18|||703/802|protein_coding|1 -chr1 1226757 . G A 1577.2 . AC=12;AF=0.1000;AN=120;BaseQRankSum=-2.078;DP=939;Dels=0.00;FS=1.648;HRun=0;HaplotypeScore=0.5032;InbreedingCoeff=-0.1091;MQ=57.89;MQ0=0;MQRankSum=0.038;QD=8.76;ReadPosRankSum=0.677;EFF=DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Agc|G562S|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Agc|G562S|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Agc|G593S|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Agc|G628S|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggc/Agc|G726S|802|SCNN1D|protein_coding|CODING|ENST00000379116|);CSQ=missense_variant|Ggc/Agc|G/S|ENSG00000162572|SCNN1D|ENST00000338555|15/15|benign(0.125)|tolerated(0.39)|562/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,missense_variant|Ggc/Agc|G/S|ENSG00000162572|SCNN1D|ENST00000325425|15/15|benign(0.276)|tolerated(0.4)|628/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,missense_variant|Ggc/Agc|G/S|ENSG00000162572|SCNN1D|ENST00000400928|14/14|benign(0.125)|tolerated(0.39)|562/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,missense_variant|Ggc/Agc|G/S|ENSG00000162572|SCNN1D|ENST00000379116|18/18|benign(0.276)|tolerated(0.4)|726/802|protein_coding|1 -chr1 1226851 . C T 188.21 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-1.990;DP=1115;Dels=0.00;FS=5.214;HRun=0;HaplotypeScore=0.5651;InbreedingCoeff=-0.0104;MQ=58.68;MQ0=0;MQRankSum=-1.264;QD=9.41;ReadPosRankSum=-0.899;EFF=DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|processed_transcript|CODING|ENST00000379037|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000476572|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P593L|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P593L|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P624L|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P659L|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cCg/cTg|P757L|802|SCNN1D|protein_coding|CODING|ENST00000379116|);CSQ=missense_variant|cCg/cTg|P/L|ENSG00000162572|SCNN1D|ENST00000338555|15/15|benign(0.007)|deleterious(0.05)|593/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000476572|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000379037|||||processed_transcript|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,missense_variant|cCg/cTg|P/L|ENSG00000162572|SCNN1D|ENST00000325425|15/15|benign(0.005)|tolerated(0.09)|659/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,missense_variant|cCg/cTg|P/L|ENSG00000162572|SCNN1D|ENST00000400928|14/14|benign(0.007)|deleterious(0.05)|593/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,missense_variant|cCg/cTg|P/L|ENSG00000162572|SCNN1D|ENST00000379116|18/18|benign(0.005)|deleterious(0.02)|757/802|protein_coding|1 -chr1 1226889 . G A 2836.8 . AC=12;AF=0.1000;AN=120;BaseQRankSum=-2.929;DP=1114;Dels=0.00;FS=6.315;HRun=0;HaplotypeScore=0.3672;InbreedingCoeff=-0.1141;MQ=58.73;MQ0=0;MQRankSum=0.148;QD=15.76;ReadPosRankSum=0.535;EFF=DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|processed_transcript|CODING|ENST00000379037|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000476572|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggg/Agg|G606R|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggg/Agg|G606R|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggg/Agg|G637R|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggg/Agg|G672R|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|Ggg/Agg|G770R|802|SCNN1D|protein_coding|CODING|ENST00000379116|);CSQ=missense_variant|Ggg/Agg|G/R|ENSG00000162572|SCNN1D|ENST00000338555|15/15|benign(0.159)|tolerated(0.42)|606/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000476572|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000379037|||||processed_transcript|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,missense_variant|Ggg/Agg|G/R|ENSG00000162572|SCNN1D|ENST00000325425|15/15|benign(0.001)|tolerated(0.38)|672/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,missense_variant|Ggg/Agg|G/R|ENSG00000162572|SCNN1D|ENST00000400928|14/14|benign(0.159)|tolerated(0.42)|606/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,missense_variant|Ggg/Agg|G/R|ENSG00000162572|SCNN1D|ENST00000379116|18/18|benign(0.001)|tolerated(0.08)|770/802|protein_coding|1 -chr1 1226971 . T C 63.36 . AC=1;AF=0.0085;AN=118;BaseQRankSum=-1.160;DP=1268;Dels=0.00;FS=4.729;HRun=4;HaplotypeScore=0.4672;InbreedingCoeff=-0.0189;MQ=58.51;MQ0=0;MQRankSum=1.085;QD=3.73;ReadPosRankSum=-0.628;EFF=DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|processed_transcript|CODING|ENST00000379037|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000476572|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cTt/cCt|L633P|638|SCNN1D|protein_coding|CODING|ENST00000338555|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cTt/cCt|L633P|638|SCNN1D|protein_coding|CODING|ENST00000400928|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cTt/cCt|L664P|669|SCNN1D|protein_coding|CODING|ENST00000379110|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cTt/cCt|L699P|704|SCNN1D|protein_coding|CODING|ENST00000325425|),NON_SYNONYMOUS_CODING(MODERATE|MISSENSE|cTt/cCt|L797P|802|SCNN1D|protein_coding|CODING|ENST00000379116|);CSQ=missense_variant|cTt/cCt|L/P|ENSG00000162572|SCNN1D|ENST00000338555|15/15|benign(0.02)|tolerated(0.18)|633/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000476572|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000379037|||||processed_transcript|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,missense_variant|cTt/cCt|L/P|ENSG00000162572|SCNN1D|ENST00000325425|15/15|benign(0.189)|tolerated(0.21)|699/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,missense_variant|cTt/cCt|L/P|ENSG00000162572|SCNN1D|ENST00000400928|14/14|benign(0.02)|tolerated(0.18)|633/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,missense_variant|cTt/cCt|L/P|ENSG00000162572|SCNN1D|ENST00000379116|18/18|benign(0.189)|deleterious(0.04)|797/802|protein_coding|1 -chr1 1227042 . G A 57.76 . AC=1;AF=0.0083;AN=120;BaseQRankSum=-0.393;DP=957;Dels=0.00;FS=2.494;HRun=0;HaplotypeScore=0.5259;InbreedingCoeff=-0.0359;MQ=58.61;MQ0=0;MQRankSum=0.096;QD=14.44;ReadPosRankSum=2.314;EFF=DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|processed_transcript|CODING|ENST00000379037|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000476572|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|),UTR_3_PRIME(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|15),UTR_3_PRIME(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|14),UTR_3_PRIME(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|20),UTR_3_PRIME(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|15),UTR_3_PRIME(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|18);CSQ=3_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000338555|15/15|||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000476572|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000379037|||||processed_transcript|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,3_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000325425|15/15|||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,3_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000400928|14/14|||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000379116|18/18|||-/802|protein_coding|1 -chr1 1227196 . A G 1166.6 . AC=2;AF=0.024;AN=82;BaseQRankSum=1.195;DP=1062;Dels=0.00;FS=10.378;HRun=6;HaplotypeScore=1.5050;InbreedingCoeff=-0.0572;MQ=57.57;MQ0=0;MQRankSum=-0.308;QD=10.42;ReadPosRankSum=0.286;EFF=DOWNSTREAM(MODIFIER||||759|ACAP3|protein_coding|CODING|ENST00000353662|),DOWNSTREAM(MODIFIER||||834|ACAP3|protein_coding|CODING|ENST00000354700|),DOWNSTREAM(MODIFIER|||||ACAP3|processed_transcript|CODING|ENST00000379037|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000467278|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000470659|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000476572|),DOWNSTREAM(MODIFIER|||||ACAP3|retained_intron|CODING|ENST00000492936|),DOWNSTREAM(MODIFIER|||||SCNN1D|nonsense_mediated_decay|CODING|ENST00000379101|),UTR_3_PRIME(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000338555|15),UTR_3_PRIME(MODIFIER||||638|SCNN1D|protein_coding|CODING|ENST00000400928|14),UTR_3_PRIME(MODIFIER||||669|SCNN1D|protein_coding|CODING|ENST00000379110|20),UTR_3_PRIME(MODIFIER||||704|SCNN1D|protein_coding|CODING|ENST00000325425|15),UTR_3_PRIME(MODIFIER||||802|SCNN1D|protein_coding|CODING|ENST00000379116|18);CSQ=3_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000338555|15/15|||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000467278|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000492936|||||retained_intron|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379101||||-/328|nonsense_mediated_decay|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000476572|||||retained_intron|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000379037|||||processed_transcript|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000354700||||-/834|protein_coding|1,3_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000325425|15/15|||-/704|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000353662||||-/759|protein_coding|1,downstream_gene_variant|||ENSG00000162572|SCNN1D|ENST00000379099||||-/308|protein_coding|1,3_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000400928|14/14|||-/638|protein_coding|1,downstream_gene_variant|||ENSG00000131584|ACAP3|ENST00000470659|||||retained_intron|1,3_prime_UTR_variant|||ENSG00000162572|SCNN1D|ENST00000379116|18/18|||-/802|protein_coding|1 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zr*GLEA1SBp^xJ#lcgkryUGl5=XgO`CFWeu$TTa{QLEps3$!R;i^gw))oVL^NAB^88 zr|tA--^TBk({{Sfq4;DuZKtn35`R!m+vyqq8=oSl?ezP{;#1|coo-YjdrPOwX*=Do zWPFC4w$ocm$Nwm&?R3?j#UGW^cDhpK_*^+{r!T1*pC_m7^p7>-^X0UiKBHFraXD?L zZ>SSrAgAs0{Ce?)a@tOBtsh?`r|mR5VwcKkJI&tMXXWgUwbQpP%s$uW)H4|Ic=x6uZka$({}oz)$yZp+D;FC zDSk{&+vzV}i61v#--#-;ovynkUQ$lm>0WE&KbO;XdegdiMLBJ!_k0>ZQBK?G$)CsT z$Z0$M&dzu}Ic=xeF?*Vvw$tpL{gs^Evvzvbq1@k0PTT2ihvUuVw4FY1B!0e}w$s1* zKHf@B+v!_>h_{o|cDi55rvLQ5;0xunojy<|-pTJTbn*KO-MC!5vz)fmiz~!?$UiLi z?|q|m#qxR9Q%?Il-TTD&Wpdh1Z>SuT2<;)8pZp|X*+#SllVY6ZKvNlD?V8MLW{JW zKC5+}4UyA!df)}|p>oeC#UW7U#^QkAgAqgg}(8J<+PpddP97woVL^V-WZ=Qr|tCi0r44f z+D>0SC_Z0K+v&x(#TUqFJ6-mU_+mM2r&kV*KP{*2^xom|6>{26H@q{xQcm0HS!3eQ z$Z0#h<(~K|Ic=vakB>hmr|opZ3GwIs{z4bOztG(##$S-rc6#!p_)BuyPOrW%{)(Ko z(?t)&*T`u*eal1fH{`UPUi@%;y_~kwCryk0RZiRK$_3r|tCC1@YZ-+D Date: Wed, 30 Aug 2023 17:21:52 -0700 Subject: [PATCH 101/116] Consolidate alignment track menu options --- .../org/broad/igv/sam/AlignmentTrackMenu.java | 88 ++++++++----------- 1 file changed, 38 insertions(+), 50 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index cedb8acd8b..a95dadda3b 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -89,9 +89,7 @@ class AlignmentTrackMenu extends IGVPopupMenu { Collection tracks = List.of(alignmentTrack); addSeparator(); add(TrackMenuUtils.getTrackRenameItem(tracks)); - addCopyToClipboardItem(e, clickedAlignment); - addSeparator(); JMenuItem item = new JMenuItem("Change Track Color..."); item.addActionListener(evt -> TrackMenuUtils.changeTrackColor(tracks)); add(item); @@ -99,9 +97,9 @@ class AlignmentTrackMenu extends IGVPopupMenu { // Experiment type (RNA, THIRD GEN, OTHER) addSeparator(); addExperimentTypeMenuItem(); - if (alignmentTrack.getExperimentType() == AlignmentTrack.ExperimentType.THIRD_GEN) { - addHaplotype(e); - } +// if (alignmentTrack.getExperimentType() == AlignmentTrack.ExperimentType.THIRD_GEN) { +// addHaplotype(e); +// } // Linked read items addLinkedReadItems(); @@ -124,16 +122,17 @@ class AlignmentTrackMenu extends IGVPopupMenu { showAllItem.addActionListener(new Deselector(showAllItem, misMatchesItem)); // Paired end items - addSeparator(); - addViewAsPairsMenuItem(); - if (clickedAlignment != null) { - addGoToMate(e, clickedAlignment); - showMateRegion(e, clickedAlignment); + if(dataManager.isPairedEnd()) { + addSeparator(); + addViewAsPairsMenuItem(); + if (clickedAlignment != null) { + addGoToMate(e, clickedAlignment); + showMateRegion(e, clickedAlignment); + } + addInsertSizeMenuItem(); } - addInsertSizeMenuItem(); // Third gen (primarily) items - addSeparator(); addThirdGenItems(clickedAlignment, e); // Display mode items @@ -147,6 +146,7 @@ class AlignmentTrackMenu extends IGVPopupMenu { // Copy items addSeparator(); + addCopyToClipboardItem(e, clickedAlignment); addCopySequenceItems(e); addConsensusSequence(e); @@ -163,11 +163,13 @@ class AlignmentTrackMenu extends IGVPopupMenu { } // Sashimi plot - addSeparator(); - JMenuItem sashimi = new JMenuItem("Sashimi Plot"); - sashimi.addActionListener(e1 -> SashimiPlot.openSashimiPlot()); - sashimi.setEnabled(alignmentTrack.getExperimentType() == AlignmentTrack.ExperimentType.RNA); - add(sashimi); + if(alignmentTrack.getExperimentType() == AlignmentTrack.ExperimentType.RNA) { + addSeparator(); + JMenuItem sashimi = new JMenuItem("Sashimi Plot"); + sashimi.addActionListener(e1 -> SashimiPlot.openSashimiPlot()); + sashimi.setEnabled(alignmentTrack.getExperimentType() == AlignmentTrack.ExperimentType.RNA); + add(sashimi); + } // Show alignments, coverage, splice junctions addSeparator(); @@ -744,7 +746,7 @@ void addPackMenuItem() { void addCopyToClipboardItem(final TrackClickEvent te, Alignment alignment) { final MouseEvent me = te.getMouseEvent(); - JMenuItem item = new JMenuItem("Copy read details to clipboard"); + JMenuItem item = new JMenuItem("Copy read details"); final ReferenceFrame frame = te.getFrame(); if (frame == null) { item.setEnabled(false); @@ -825,17 +827,6 @@ JMenuItem addShowAllBasesMenuItem() { return item; } - void addQuickConsensusModeItem() { - // Change track height by attribute - final JMenuItem item = new JCheckBoxMenuItem("Quick consensus mode"); - item.setSelected(renderOptions.isQuickConsensusMode()); - item.addActionListener(aEvt -> { - renderOptions.setQuickConsensusMode(item.isSelected()); - alignmentTrack.repaint(); - }); - add(item); - } - JMenuItem addShowMismatchesMenuItem() { // Change track height by attribute final JMenuItem item = new JCheckBoxMenuItem("Show mismatched bases"); @@ -1075,7 +1066,6 @@ void addExtViewItem(final TrackClickEvent te) { * and linking by arbitrary tag. */ void addLinkedReadItems() { - addSeparator(); addSeparator(); final JCheckBoxMenuItem supplementalItem = new JCheckBoxMenuItem("Link supplementary alignments"); @@ -1160,36 +1150,33 @@ void addThirdGenItems(Alignment clickedAlignment, final TrackClickEvent tce) { addShowChimericRegions(alignmentTrack, tce, clickedAlignment); addShowDiagram(tce, clickedAlignment); - final JMenuItem qcItem = new JCheckBoxMenuItem("Quick consensus mode"); - qcItem.setSelected(renderOptions.isQuickConsensusMode()); - qcItem.addActionListener(aEvt -> { - renderOptions.setQuickConsensusMode(qcItem.isSelected()); - alignmentTrack.repaint(); - }); +// final JMenuItem qcItem = new JCheckBoxMenuItem("Quick consensus mode"); +// qcItem.setSelected(renderOptions.isQuickConsensusMode()); +// qcItem.addActionListener(aEvt -> { +// renderOptions.setQuickConsensusMode(qcItem.isSelected()); +// alignmentTrack.repaint(); +// }); - final JMenuItem thresholdItem = new JMenuItem("Small indel threshold..."); - thresholdItem.addActionListener(evt -> UIUtilities.invokeOnEventThread(() -> { - String sith = MessageUtils.showInputDialog("Small indel threshold: ", String.valueOf(renderOptions.getSmallIndelThreshold())); - try { - renderOptions.setSmallIndelThreshold(Integer.parseInt(sith)); - alignmentTrack.repaint(); - } catch (NumberFormatException e) { - log.error("Error setting small indel threshold - not an integer", e); - } - })); - thresholdItem.setEnabled(renderOptions.isHideSmallIndels()); final JMenuItem item = new JCheckBoxMenuItem("Hide small indels"); item.setSelected(renderOptions.isHideSmallIndels()); item.addActionListener(aEvt -> UIUtilities.invokeOnEventThread(() -> { + + if(item.isSelected()) { + String sith = MessageUtils.showInputDialog("Small indel threshold: ", String.valueOf(renderOptions.getSmallIndelThreshold())); + try { + renderOptions.setSmallIndelThreshold(Integer.parseInt(sith)); + } catch (NumberFormatException e) { + log.error("Error setting small indel threshold - not an integer", e); + } + } + renderOptions.setHideSmallIndels(item.isSelected()); - thresholdItem.setEnabled(item.isSelected()); alignmentTrack.repaint(); })); - add(qcItem); + //add(qcItem); add(item); - add(thresholdItem); } /** @@ -1383,3 +1370,4 @@ public void actionPerformed(ActionEvent e) { } } + From fd56590261398ac98944c118787bb3c775184d0d Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 30 Aug 2023 21:25:15 -0700 Subject: [PATCH 102/116] Move "export track names" from popup to "Tracks" menu --- .../org/broad/igv/track/TrackMenuUtils.java | 49 ---------------- .../java/org/broad/igv/ui/IGVMenuBar.java | 58 ++++++++++++++++++- .../igv/ui/panel/TrackPanelComponent.java | 4 -- 3 files changed, 56 insertions(+), 55 deletions(-) diff --git a/src/main/java/org/broad/igv/track/TrackMenuUtils.java b/src/main/java/org/broad/igv/track/TrackMenuUtils.java index 01a8f3c138..7cf1e9601f 100644 --- a/src/main/java/org/broad/igv/track/TrackMenuUtils.java +++ b/src/main/java/org/broad/igv/track/TrackMenuUtils.java @@ -1205,55 +1205,6 @@ public static void changeBasePairTrackColor(final List tracks, } - public static void exportTrackNames(final Collection selectedTracks) { - - if (selectedTracks.isEmpty()) { - return; - } - - File file = FileDialogUtils.chooseFile("Export track names", - PreferencesManager.getPreferences().getLastTrackDirectory(), - new File("trackNames.tab"), - FileDialogUtils.SAVE); - - if (file == null) { - return; - } - - PrintWriter pw = null; - try { - pw = new PrintWriter(new BufferedWriter(new FileWriter(file))); - - List attributes = AttributeManager.getInstance().getVisibleAttributes(); - - pw.print("Name"); - for (String att : attributes) { - pw.print("\t" + att); - } - pw.println(); - - for (Track track : selectedTracks) { - //We preserve the alpha value. This is motivated by MergedTracks - pw.print(track.getName()); - - for (String att : attributes) { - String val = track.getAttributeValue(att); - pw.print("\t" + (val == null ? "" : val)); - } - pw.println(); - } - - - } catch (IOException e) { - MessageUtils.showErrorMessage("Error writing to file", e); - log.error(e); - } finally { - if (pw != null) pw.close(); - } - - } - - public static JMenuItem getCopyDetailsItem(final Feature f, final TrackClickEvent evt) { JMenuItem item = new JMenuItem("Copy Details to Clipboard"); item.addActionListener(new ActionListener() { diff --git a/src/main/java/org/broad/igv/ui/IGVMenuBar.java b/src/main/java/org/broad/igv/ui/IGVMenuBar.java index 20984bee6a..8b0e0e5541 100644 --- a/src/main/java/org/broad/igv/ui/IGVMenuBar.java +++ b/src/main/java/org/broad/igv/ui/IGVMenuBar.java @@ -36,6 +36,8 @@ import org.broad.igv.event.IGVEventObserver; import org.broad.igv.feature.genome.GenomeManager; import org.broad.igv.feature.genome.GenomeUtils; +import org.broad.igv.track.AttributeManager; +import org.broad.igv.track.Track; import org.broad.igv.util.GoogleUtils; import org.broad.igv.oauth.OAuthProvider; import org.broad.igv.oauth.OAuthUtils; @@ -69,10 +71,10 @@ import java.awt.event.ActionEvent; import java.awt.event.ActionListener; import java.awt.event.KeyEvent; -import java.io.File; -import java.io.IOException; +import java.io.*; import java.net.URI; import java.util.ArrayList; +import java.util.Collection; import java.util.List; import static org.broad.igv.prefs.Constants.*; @@ -512,6 +514,9 @@ private JMenu createTracksMenu() { menuAction.setToolTipText(UIConstants.OVERLAY_TRACKS_TOOLTIP); menuItems.add(MenuAndToolbarUtils.createMenuItem(menuAction)); + JMenuItem exportNames = new JMenuItem("Export Track Names..."); + exportNames.addActionListener(e12 -> exportTrackNames(IGV.getInstance().getAllTracks())); + menuItems.add(exportNames); menuItems.add(new JSeparator()); @@ -1182,4 +1187,53 @@ public static JMenuItem createBlatMenuItem() { return menuItem; } + + private void exportTrackNames(final Collection selectedTracks) { + + if (selectedTracks.isEmpty()) { + return; + } + + File file = FileDialogUtils.chooseFile("Export track names", + PreferencesManager.getPreferences().getLastTrackDirectory(), + new File("trackNames.txt"), + FileDialogUtils.SAVE); + + if (file == null) { + return; + } + + PrintWriter pw = null; + try { + pw = new PrintWriter(new BufferedWriter(new FileWriter(file))); + + List attributes = AttributeManager.getInstance().getVisibleAttributes(); + + pw.print("Name"); + for (String att : attributes) { + pw.print("\t" + att); + } + pw.println(); + + for (Track track : selectedTracks) { + //We preserve the alpha value. This is motivated by MergedTracks + pw.print(track.getName()); + + for (String att : attributes) { + String val = track.getAttributeValue(att); + pw.print("\t" + (val == null ? "" : val)); + } + pw.println(); + } + + + } catch (IOException e) { + MessageUtils.showErrorMessage("Error writing to file", e); + log.error(e); + } finally { + if (pw != null) pw.close(); + } + + } + } diff --git a/src/main/java/org/broad/igv/ui/panel/TrackPanelComponent.java b/src/main/java/org/broad/igv/ui/panel/TrackPanelComponent.java index d4d250999d..be7dcad47e 100644 --- a/src/main/java/org/broad/igv/ui/panel/TrackPanelComponent.java +++ b/src/main/java/org/broad/igv/ui/panel/TrackPanelComponent.java @@ -230,10 +230,6 @@ protected void openPopupMenu(TrackClickEvent te, List extraItems) { JMenuItem exportFeats = TrackMenuUtils.getExportFeatures(selectedTracks, frame); if (exportFeats != null) menu.add(exportFeats); - JMenuItem exportNames = new JMenuItem("Export track names..."); - exportNames.addActionListener(e12 -> TrackMenuUtils.exportTrackNames(selectedTracks)); - menu.add(exportNames); - menu.addSeparator(); menu.add(TrackMenuUtils.getRemoveMenuItem(selectedTracks)); } From 6acdd9f2814a038b5344146af9f2f1528050dc06 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Wed, 30 Aug 2023 21:27:54 -0700 Subject: [PATCH 103/116] Add menu item and batch command to group alignments by insertion at a specific position. The grouping considers insertions either side of the specified position. Fixes #1380 --- .../org/broad/igv/batch/CommandExecutor.java | 8 +++- .../org/broad/igv/sam/AlignmentPacker.java | 33 +++++++++++++- .../org/broad/igv/sam/AlignmentTrack.java | 10 ++++- .../org/broad/igv/sam/AlignmentTrackMenu.java | 44 ++++++++++++------- 4 files changed, 76 insertions(+), 19 deletions(-) diff --git a/src/main/java/org/broad/igv/batch/CommandExecutor.java b/src/main/java/org/broad/igv/batch/CommandExecutor.java index da096b261c..29e4c38be0 100755 --- a/src/main/java/org/broad/igv/batch/CommandExecutor.java +++ b/src/main/java/org/broad/igv/batch/CommandExecutor.java @@ -1110,7 +1110,7 @@ private String sort(String sortArg, String param2, String param3, String param4) private String group(String groupArg, String tagArg) { final AlignmentTrack.GroupOption groupOption = getAlignmentGroupOption(groupArg); Range r = null; - if (groupOption == AlignmentTrack.GroupOption.BASE_AT_POS) { + if (groupOption == AlignmentTrack.GroupOption.BASE_AT_POS || groupOption == AlignmentTrack.GroupOption.INSERTION_AT_POS) { if (tagArg == null) { return "Error: position is required"; } else { @@ -1261,7 +1261,11 @@ private static AlignmentTrack.GroupOption getAlignmentGroupOption(String str) { return AlignmentTrack.GroupOption.LIBRARY; } else if (str.equalsIgnoreCase("readGroup") || str.equalsIgnoreCase("read_group")) { return AlignmentTrack.GroupOption.READ_GROUP; - } else { + } else if (str.equalsIgnoreCase("base")) { + return AlignmentTrack.GroupOption.BASE_AT_POS; + }else if (str.equalsIgnoreCase("insertion")) { + return AlignmentTrack.GroupOption.INSERTION_AT_POS; + }else { try { return AlignmentTrack.GroupOption.valueOf(str.toUpperCase()); } catch (IllegalArgumentException e) { diff --git a/src/main/java/org/broad/igv/sam/AlignmentPacker.java b/src/main/java/org/broad/igv/sam/AlignmentPacker.java index 009ca3b854..ffd75089f0 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentPacker.java +++ b/src/main/java/org/broad/igv/sam/AlignmentPacker.java @@ -70,6 +70,7 @@ public PackedAlignments packAlignments( LinkedHashMap> packedAlignments = new LinkedHashMap>(); List alList = interval.getAlignments(); + // TODO -- means to undo this if (renderOptions.isLinkedReads()) { alList = linkByTag(alList, renderOptions.getLinkByTag()); @@ -93,9 +94,15 @@ public PackedAlignments packAlignments( } - // Now alphabetize (sort) and pack the groups + // Now sort the groups by their name and pack each group individually List keys = new ArrayList(groupedAlignments.keySet()); Comparator groupComparator = getGroupComparator(renderOptions.getGroupByOption()); + + // Certain group options sort descending by default, as indicated by the "reverse" property + if(renderOptions.getGroupByOption().reverse) { + groupComparator = groupComparator.reversed(); + } + if(renderOptions.isInvertGroupSorting()){ groupComparator = groupComparator.reversed(); } @@ -459,6 +466,30 @@ private Object getGroupValue(Alignment al, AlignmentTrack.RenderOptions renderOp } else { // does not overlap position return "3:"; } + case INSERTION_AT_POS: + // Use a string prefix to enforce grouping rules: + // 1: alignments with a base at the position + // 2: alignments with a gap at the position + // 3: alignment that do not overlap the position (or are on a different chromosome) + if (pos != null && + al.getChr().equals(pos.getChr()) && + al.getAlignmentStart() <= pos.getStart() && + al.getAlignmentEnd() > pos.getStart()) { + int insertionBaseCount = 0; + AlignmentBlock leftInsertion = al.getInsertionAt(pos.getStart() + 1); + if(leftInsertion != null) { + insertionBaseCount += leftInsertion.getLength(); + } + AlignmentBlock rightInsertion = al.getInsertionAt(pos.getStart()); + if(rightInsertion != null) { + insertionBaseCount += rightInsertion.getLength(); + } + return insertionBaseCount; + + } else { + return 0; + } + case MOVIE: // group PacBio reads by movie readNameParts = al.getReadName().split("/"); if (readNameParts.length < 3) { diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrack.java b/src/main/java/org/broad/igv/sam/AlignmentTrack.java index 1c8fa08b10..4469b705c5 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrack.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrack.java @@ -155,6 +155,7 @@ public enum GroupOption { CHIMERIC("chimeric"), SUPPLEMENTARY("supplementary flag"), BASE_AT_POS("base at position"), + INSERTION_AT_POS("insertion at position", true), MOVIE("movie"), ZMW("ZMW"), HAPLOTYPE("haplotype"), @@ -165,9 +166,16 @@ public enum GroupOption { MAPPING_QUALITY("mapping quality"); public final String label; + public final boolean reverse; GroupOption(String label) { this.label = label; + this.reverse = false; + } + + GroupOption(String label, boolean reverse) { + this.label = label; + this.reverse = reverse; } } @@ -780,7 +788,7 @@ public void groupAlignments(GroupOption option, String tag, Range pos) { if (option == GroupOption.TAG && tag != null) { renderOptions.setGroupByTag(tag); } - if (option == GroupOption.BASE_AT_POS && pos != null) { + if ((option == GroupOption.BASE_AT_POS || option == GroupOption.INSERTION_AT_POS) && pos != null) { renderOptions.setGroupByPos(pos); } renderOptions.setGroupByOption(option); diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index a95dadda3b..f52e96326b 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -400,10 +400,14 @@ void addGroupMenuItem(final TrackClickEvent te) {//ReferenceFrame frame) { ButtonGroup group = new ButtonGroup(); AlignmentTrack.GroupOption[] groupOptions = { - AlignmentTrack.GroupOption.NONE, AlignmentTrack.GroupOption.STRAND, AlignmentTrack.GroupOption.FIRST_OF_PAIR_STRAND, AlignmentTrack.GroupOption.SAMPLE, - AlignmentTrack.GroupOption.LIBRARY, AlignmentTrack.GroupOption.READ_GROUP, AlignmentTrack.GroupOption.MATE_CHROMOSOME, - AlignmentTrack.GroupOption.PAIR_ORIENTATION, AlignmentTrack.GroupOption.CHIMERIC, AlignmentTrack.GroupOption.SUPPLEMENTARY, AlignmentTrack.GroupOption.REFERENCE_CONCORDANCE, - AlignmentTrack.GroupOption.MOVIE, AlignmentTrack.GroupOption.ZMW, AlignmentTrack.GroupOption.READ_ORDER, AlignmentTrack.GroupOption.LINKED, AlignmentTrack.GroupOption.PHASE, + AlignmentTrack.GroupOption.NONE, AlignmentTrack.GroupOption.STRAND, + AlignmentTrack.GroupOption.FIRST_OF_PAIR_STRAND, AlignmentTrack.GroupOption.SAMPLE, + AlignmentTrack.GroupOption.LIBRARY, AlignmentTrack.GroupOption.READ_GROUP, + AlignmentTrack.GroupOption.MATE_CHROMOSOME, + AlignmentTrack.GroupOption.PAIR_ORIENTATION, AlignmentTrack.GroupOption.CHIMERIC, + AlignmentTrack.GroupOption.SUPPLEMENTARY, AlignmentTrack.GroupOption.REFERENCE_CONCORDANCE, + AlignmentTrack.GroupOption.MOVIE, AlignmentTrack.GroupOption.ZMW, AlignmentTrack.GroupOption.READ_ORDER, + AlignmentTrack.GroupOption.LINKED, AlignmentTrack.GroupOption.PHASE, AlignmentTrack.GroupOption.MAPPING_QUALITY }; @@ -433,16 +437,16 @@ void addGroupMenuItem(final TrackClickEvent te) {//ReferenceFrame frame) { groupMenu.add(tagOption); group.add(tagOption); - Range oldGroupByPos = renderOptions.getGroupByPos(); - if (oldGroupByPos != null && renderOptions.getGroupByOption() == AlignmentTrack.GroupOption.BASE_AT_POS) { // already sorted by the base at a position - JCheckBoxMenuItem oldGroupByPosOption = new JCheckBoxMenuItem("base at " + oldGroupByPos.getChr() + - ":" + Globals.DECIMAL_FORMAT.format(1 + oldGroupByPos.getStart())); - groupMenu.add(oldGroupByPosOption); - oldGroupByPosOption.setSelected(true); - } - - if (renderOptions.getGroupByOption() != AlignmentTrack.GroupOption.BASE_AT_POS || oldGroupByPos == null || - !oldGroupByPos.getChr().equals(chrom) || oldGroupByPos.getStart() != chromStart) { // not already sorted by this position +// Range oldGroupByPos = renderOptions.getGroupByPos(); +// if (oldGroupByPos != null && renderOptions.getGroupByOption() == AlignmentTrack.GroupOption.BASE_AT_POS) { // already sorted by the base at a position +// JCheckBoxMenuItem oldGroupByPosOption = new JCheckBoxMenuItem("base at " + oldGroupByPos.getChr() + +// ":" + Globals.DECIMAL_FORMAT.format(1 + oldGroupByPos.getStart())); +// groupMenu.add(oldGroupByPosOption); +// oldGroupByPosOption.setSelected(true); +// } +// +// if (renderOptions.getGroupByOption() != AlignmentTrack.GroupOption.BASE_AT_POS || oldGroupByPos == null || +// !oldGroupByPos.getChr().equals(chrom) || oldGroupByPos.getStart() != chromStart) { // not already sorted by this position JCheckBoxMenuItem newGroupByPosOption = new JCheckBoxMenuItem("base at " + chrom + ":" + Globals.DECIMAL_FORMAT.format(1 + chromStart)); newGroupByPosOption.addActionListener(aEvt -> { @@ -451,7 +455,17 @@ void addGroupMenuItem(final TrackClickEvent te) {//ReferenceFrame frame) { }); groupMenu.add(newGroupByPosOption); group.add(newGroupByPosOption); - } + // } + + JCheckBoxMenuItem newGroupByInsOption = new JCheckBoxMenuItem("insertion at " + chrom + + ":" + Globals.DECIMAL_FORMAT.format(1 + chromStart)); + newGroupByInsOption.addActionListener(aEvt -> { + Range groupByPos = new Range(chrom, chromStart, chromStart + 1); + groupAlignments(AlignmentTrack.GroupOption.INSERTION_AT_POS, null, groupByPos); + }); + groupMenu.add(newGroupByInsOption); + group.add(newGroupByInsOption); + groupMenu.add(new Separator()); JCheckBoxMenuItem invertGroupNameSortingOption = new JCheckBoxMenuItem("Reverse group order"); From a47f1ffe507b85cd0f6f21f6ef65c2b6f57091b9 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 31 Aug 2023 12:02:08 -0700 Subject: [PATCH 104/116] menu tweaks --- .../org/broad/igv/sam/AlignmentTrackMenu.java | 54 ++++++++----------- .../java/org/broad/igv/ui/IGVMenuBar.java | 13 +++-- 2 files changed, 33 insertions(+), 34 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index f52e96326b..0994e5f76b 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -101,8 +101,6 @@ class AlignmentTrackMenu extends IGVPopupMenu { // addHaplotype(e); // } - // Linked read items - addLinkedReadItems(); // Group, sort, color, shade, and pack addSeparator(); @@ -121,6 +119,24 @@ class AlignmentTrackMenu extends IGVPopupMenu { misMatchesItem.addActionListener(new Deselector(misMatchesItem, showAllItem)); showAllItem.addActionListener(new Deselector(showAllItem, misMatchesItem)); + + // Hide small indels + JMenuItem smallIndelsItem = new JCheckBoxMenuItem("Hide small indels"); + smallIndelsItem.setSelected(renderOptions.isHideSmallIndels()); + smallIndelsItem.addActionListener(aEvt -> UIUtilities.invokeOnEventThread(() -> { + if(smallIndelsItem.isSelected()) { + String sith = MessageUtils.showInputDialog("Small indel threshold: ", String.valueOf(renderOptions.getSmallIndelThreshold())); + try { + renderOptions.setSmallIndelThreshold(Integer.parseInt(sith)); + } catch (NumberFormatException exc) { + log.error("Error setting small indel threshold - not an integer", exc); + } + } + renderOptions.setHideSmallIndels(smallIndelsItem.isSelected()); + alignmentTrack.repaint(); + })); + add(smallIndelsItem); + // Paired end items if(dataManager.isPairedEnd()) { addSeparator(); @@ -133,6 +149,7 @@ class AlignmentTrackMenu extends IGVPopupMenu { } // Third gen (primarily) items + addSeparator(); addThirdGenItems(clickedAlignment, e); // Display mode items @@ -1081,7 +1098,6 @@ void addExtViewItem(final TrackClickEvent te) { */ void addLinkedReadItems() { - addSeparator(); final JCheckBoxMenuItem supplementalItem = new JCheckBoxMenuItem("Link supplementary alignments"); supplementalItem.setSelected(alignmentTrack.isLinkedReads() && "READNAME".equals(renderOptions.getLinkByTag())); supplementalItem.addActionListener(aEvt -> { @@ -1160,37 +1176,13 @@ private void addInsertionItems(AlignmentBlock insertion) { void addThirdGenItems(Alignment clickedAlignment, final TrackClickEvent tce) { - //Supplementary/chimeric items, only if the read has an SA tag + // Linked read items -- mostly for 3rd gen but might also be relevant to 10X and other linked read assays + addLinkedReadItems(); + + //Supplementary/chimeric items, only if the read has an SA tag; addShowChimericRegions(alignmentTrack, tce, clickedAlignment); addShowDiagram(tce, clickedAlignment); -// final JMenuItem qcItem = new JCheckBoxMenuItem("Quick consensus mode"); -// qcItem.setSelected(renderOptions.isQuickConsensusMode()); -// qcItem.addActionListener(aEvt -> { -// renderOptions.setQuickConsensusMode(qcItem.isSelected()); -// alignmentTrack.repaint(); -// }); - - - final JMenuItem item = new JCheckBoxMenuItem("Hide small indels"); - item.setSelected(renderOptions.isHideSmallIndels()); - item.addActionListener(aEvt -> UIUtilities.invokeOnEventThread(() -> { - - if(item.isSelected()) { - String sith = MessageUtils.showInputDialog("Small indel threshold: ", String.valueOf(renderOptions.getSmallIndelThreshold())); - try { - renderOptions.setSmallIndelThreshold(Integer.parseInt(sith)); - } catch (NumberFormatException e) { - log.error("Error setting small indel threshold - not an integer", e); - } - } - - renderOptions.setHideSmallIndels(item.isSelected()); - alignmentTrack.repaint(); - })); - - //add(qcItem); - add(item); } /** diff --git a/src/main/java/org/broad/igv/ui/IGVMenuBar.java b/src/main/java/org/broad/igv/ui/IGVMenuBar.java index 8b0e0e5541..e380d833a0 100644 --- a/src/main/java/org/broad/igv/ui/IGVMenuBar.java +++ b/src/main/java/org/broad/igv/ui/IGVMenuBar.java @@ -514,8 +514,15 @@ private JMenu createTracksMenu() { menuAction.setToolTipText(UIConstants.OVERLAY_TRACKS_TOOLTIP); menuItems.add(MenuAndToolbarUtils.createMenuItem(menuAction)); - JMenuItem exportNames = new JMenuItem("Export Track Names..."); - exportNames.addActionListener(e12 -> exportTrackNames(IGV.getInstance().getAllTracks())); + // Export track names and attributes -- if > 1 i sselected export those, otherwise export all + JMenuItem exportNames = new JMenuItem("Export Track Names and Attributes..."); + exportNames.addActionListener(e12 -> { + Collection exportTracks = IGV.getInstance().getSelectedTracks(); + if (exportTracks.size() <= 1) { + exportTracks = IGV.getInstance().getAllTracks(); + } + exportTrackNames(exportTracks); + }); menuItems.add(exportNames); menuItems.add(new JSeparator()); @@ -1188,7 +1195,7 @@ public static JMenuItem createBlatMenuItem() { return menuItem; } - private void exportTrackNames(final Collection selectedTracks) { + private void exportTrackNames(final Collection selectedTracks) { if (selectedTracks.isEmpty()) { return; From 00c43f6a2f7eb8b0fdba8a4187c6c110283f83aa Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 31 Aug 2023 22:06:01 -0700 Subject: [PATCH 105/116] Show base modifications in expanded insertions. Fixes #1303 --- .../org/broad/igv/sam/AlignmentRenderer.java | 2 +- .../java/org/broad/igv/sam/BaseRenderer.java | 30 ++++- .../sam/mods/BaseModificationRenderer.java | 107 ++++++++++-------- 3 files changed, 86 insertions(+), 53 deletions(-) diff --git a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java index ac041e70d9..14460f2127 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentRenderer.java +++ b/src/main/java/org/broad/igv/sam/AlignmentRenderer.java @@ -864,7 +864,7 @@ private void drawAlignment( // Base modification if (colorOption.isBaseMod()) { - BaseModificationRenderer.drawModifications(alignment, bpStart, locScale, rowRect, context.getGraphics(), colorOption, renderOptions.getBasemodFilter(), renderOptions.getBasemodThreshold()); + BaseModificationRenderer.drawModifications(alignment, bpStart, locScale, rowRect, context.getGraphics(), renderOptions); } // Kinetic data diff --git a/src/main/java/org/broad/igv/sam/BaseRenderer.java b/src/main/java/org/broad/igv/sam/BaseRenderer.java index 92e6b4d3d9..b8844e6440 100644 --- a/src/main/java/org/broad/igv/sam/BaseRenderer.java +++ b/src/main/java/org/broad/igv/sam/BaseRenderer.java @@ -2,6 +2,9 @@ import org.broad.igv.renderer.GraphicUtils; import org.broad.igv.renderer.SequenceRenderer; +import org.broad.igv.sam.mods.BaseModficationFilter; +import org.broad.igv.sam.mods.BaseModificationRenderer; +import org.broad.igv.sam.mods.BaseModificationSet; import org.broad.igv.track.RenderContext; import org.broad.igv.ui.FontManager; import org.broad.igv.ui.color.ColorUtilities; @@ -77,6 +80,7 @@ public static void drawExpandedInsertions(InsertionMarker insertionMarker, Font f = FontManager.getFont(Font.BOLD, fontSize); g.setFont(f); } + AlignmentTrack.ColorOption colorOption = renderOptions.getColorOption(); for (Alignment alignment : alignments) { @@ -111,23 +115,43 @@ public static void drawExpandedInsertions(InsertionMarker insertionMarker, else if (pX + dX < rect.getX()) continue; char c = p < padding ? '-' : (char) bases.getByte(p - padding); - Color color = SequenceRenderer.nucleotideColors.get(c); + + + Color color = null; + // TODO -- support bisulfite mode? Probably not possible as that depends on the reference + //if (bisulfiteMode) { + // color = bisinfo.getDisplayColor(idx); + // } else + if (colorOption.isBaseMod() || + colorOption.isSMRTKinetics()) { + color = Color.GRAY; + } else { + //color = nucleotideColors.get(c); + color = SequenceRenderer.nucleotideColors.get(c); + } if (color == null) { color = Color.black; } + if (renderOptions.getShadeBasesOption() && p >= padding) { byte qual = p < padding ? (byte) 126 : insertion.getQuality(p - padding); color = BaseRenderer.getShadedColor(color, qual, renderOptions.getBaseQualityMin(), renderOptions.getBaseQualityMax()); } - if(dX < 8) { + if (dX < 8) { g.setColor(color); g.fill(new Rectangle2D.Double(pX, rect.y, dX, rect.height)); } else { - drawBase(g, color, c,(int) pX, rect.y, (int) dX, rect.height - (leaveMargin ? 2 : 0), false, null); + drawBase(g, color, c, (int) pX, rect.y, (int) dX, rect.height - (leaveMargin ? 2 : 0), false, null); } } insertion.setPixelRange(context.translateX + pixelStart, context.translateX + pixelEnd); + + + if (colorOption.isBaseMod()) { + List baseModificationSets = alignment.getBaseModificationSets(); + BaseModificationRenderer.drawBlock(origin, locScale, rect, g, renderOptions, baseModificationSets, insertion); + } } } } finally { diff --git a/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java b/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java index 0501e06a70..90f1e5543b 100644 --- a/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java +++ b/src/main/java/org/broad/igv/sam/mods/BaseModificationRenderer.java @@ -3,6 +3,7 @@ import org.broad.igv.sam.Alignment; import org.broad.igv.sam.AlignmentBlock; import org.broad.igv.sam.AlignmentTrack; +import org.broad.igv.sam.InsertionMarker; import java.awt.*; import java.util.List; @@ -15,9 +16,7 @@ public static void drawModifications( double locScale, Rectangle rowRect, Graphics g, - AlignmentTrack.ColorOption colorOption, - BaseModficationFilter filter, - float threshold) { + AlignmentTrack.RenderOptions renderOptions) { List baseModificationSets = alignment.getBaseModificationSets(); @@ -25,61 +24,71 @@ public static void drawModifications( if (baseModificationSets != null) { for (AlignmentBlock block : alignment.getAlignmentBlocks()) { // Compute bounds - int pY = (int) rowRect.getY(); - int dY = (int) rowRect.getHeight(); - int dX = (int) Math.max(1, (1.0 / locScale)); + drawBlock(bpStart, locScale, rowRect, g, renderOptions, baseModificationSets, block); + } + } + } - for (int i = block.getBases().startOffset; i < block.getBases().startOffset + block.getBases().length; i++) { + public static void drawBlock(double bpStart, double locScale, Rectangle rowRect, Graphics g, AlignmentTrack.RenderOptions renderOptions, List baseModificationSets, AlignmentBlock block) { - int blockIdx = i - block.getBases().startOffset; - int pX = (int) ((block.getStart() + blockIdx - bpStart) / locScale); + AlignmentTrack.ColorOption colorOption = renderOptions.getColorOption(); + BaseModficationFilter filter = renderOptions.getBasemodFilter(); + float threshold = renderOptions.getBasemodThreshold(); - // Don't draw out of clipping rect - if (pX > rowRect.getMaxX()) { - break; - } else if (pX + dX < rowRect.getX()) { - continue; - } - // Search all sets for modifications of this base. Pick modification with largest likelhiood - int maxLh = 0; - int noModLh = 255; - String modification = null; - char canonicalBase = 0; - for (BaseModificationSet bmSet : baseModificationSets) { - if (bmSet.containsPosition(i)) { - int lh = Byte.toUnsignedInt(bmSet.getLikelihoods().get(i)); - noModLh -= lh; - if ((filter == null || filter.pass(bmSet.getModification(), canonicalBase)) && (modification == null || lh > maxLh)) { - modification = bmSet.getModification(); - canonicalBase = bmSet.getCanonicalBase(); - maxLh = lh; - } - } + int pY = (int) rowRect.getY(); + int dY = (int) rowRect.getHeight(); + int dX = (int) Math.max(1, (1.0 / locScale)); + + for (int i = block.getBases().startOffset; i < block.getBases().startOffset + block.getBases().length; i++) { + + int blockIdx = i - block.getBases().startOffset; + int pX = (int) ((block.getStart() + blockIdx - bpStart) / locScale); + + // Don't draw out of clipping rect + if (pX > rowRect.getMaxX()) { + break; + } else if (pX + dX < rowRect.getX()) { + continue; + } + + // Search all sets for modifications of this base. Pick modification with largest likelhiood + int maxLh = 0; + int noModLh = 255; + String modification = null; + char canonicalBase = 0; + for (BaseModificationSet bmSet : baseModificationSets) { + if (bmSet.containsPosition(i)) { + int lh = Byte.toUnsignedInt(bmSet.getLikelihoods().get(i)); + noModLh -= lh; + if ((filter == null || filter.pass(bmSet.getModification(), canonicalBase)) && (modification == null || lh > maxLh)) { + modification = bmSet.getModification(); + canonicalBase = bmSet.getCanonicalBase(); + maxLh = lh; } + } + } - if (modification != null) { - - Color c = null; - final float scaledThreshold = threshold * 255; - if (noModLh > maxLh && colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR && noModLh >= scaledThreshold) { - c = BaseModificationColors.getModColor("NONE_" + canonicalBase, noModLh, colorOption); - } else if (maxLh >= scaledThreshold) { - c = BaseModificationColors.getModColor(modification, maxLh, colorOption); - } - if (c != null) { - g.setColor(c); - - // Expand narrow width to make more visible - if (dX < 3) { - dX = 3; - pX--; - } - g.fillRect(pX, pY, dX, Math.max(1, dY - 2)); - } + if (modification != null) { + Color c = null; + final float scaledThreshold = threshold * 255; + if (noModLh > maxLh && colorOption == AlignmentTrack.ColorOption.BASE_MODIFICATION_2COLOR && noModLh >= scaledThreshold) { + c = BaseModificationColors.getModColor("NONE_" + canonicalBase, noModLh, colorOption); + } else if (maxLh >= scaledThreshold) { + c = BaseModificationColors.getModColor(modification, maxLh, colorOption); + } + if (c != null) { + g.setColor(c); + + // Expand narrow width to make more visible + if (dX < 3) { + dX = 3; + pX--; } + g.fillRect(pX, pY, dX, Math.max(1, dY - 2)); } + } } } From ab1d42f09934c22a1596a5d14ac7809606650a4a Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Fri, 1 Sep 2023 14:53:06 -0700 Subject: [PATCH 106/116] Update locus string in splitscreen view (#1385) Update locus string in splitscreen view. Fixes #1293 --- .../java/org/broad/igv/event/ViewChange.java | 7 +---- .../java/org/broad/igv/lists/GeneList.java | 10 ------- .../org/broad/igv/sam/AlignmentTrackMenu.java | 30 +++++++++---------- src/main/java/org/broad/igv/ui/IGV.java | 2 +- .../broad/igv/ui/action/SearchCommand.java | 2 +- .../igv/ui/commandbar/IGVCommandBar.java | 19 +++++------- .../org/broad/igv/ui/panel/CytobandPanel.java | 15 +++++----- .../org/broad/igv/ui/panel/FrameManager.java | 2 +- .../org/broad/igv/ui/panel/HeaderPanel.java | 16 +++++++++- .../igv/ui/panel/HeaderPanelContainer.java | 19 +++++------- .../broad/igv/ui/panel/ReferenceFrame.java | 6 ++-- .../igv/ui/panel/RegionNavigatorDialog.java | 2 +- .../igv/ui/panel/ReferenceFrameTest.java | 2 +- .../ui/panel/ReferenceFrameTestHeaded.java | 2 +- 14 files changed, 62 insertions(+), 72 deletions(-) diff --git a/src/main/java/org/broad/igv/event/ViewChange.java b/src/main/java/org/broad/igv/event/ViewChange.java index a8e5413a67..bec16073dc 100644 --- a/src/main/java/org/broad/igv/event/ViewChange.java +++ b/src/main/java/org/broad/igv/event/ViewChange.java @@ -39,7 +39,7 @@ */ public class ViewChange { - public enum Type {Change, ChromosomeChange, LocusChange} + public enum Type {ChromosomeChange, LocusChange} boolean recordHistory = false; public Type type; @@ -71,11 +71,6 @@ public void setRecordHistory(boolean recordHistory) { this.recordHistory = recordHistory; } - - public static ViewChange Result() { - return new ViewChange(Type.Change); - } - public static ViewChange ChromosomeChangeResult(String chrName) { return new ViewChange(Type.ChromosomeChange, chrName); } diff --git a/src/main/java/org/broad/igv/lists/GeneList.java b/src/main/java/org/broad/igv/lists/GeneList.java index b9b7a0ee9e..12b91ceedc 100644 --- a/src/main/java/org/broad/igv/lists/GeneList.java +++ b/src/main/java/org/broad/igv/lists/GeneList.java @@ -41,7 +41,6 @@ public class GeneList { private String name; private String description; private List loci; - private boolean showName = true; public GeneList(String name, String description, String group, List loci) { init(name, description, group, loci); @@ -51,11 +50,6 @@ public GeneList(String name, List loci) { this(name, null, GeneListManager.USER_GROUP, loci); } - public GeneList(String name, List loci, boolean showName) { - this(name, null, GeneListManager.USER_GROUP, loci); - this.showName = showName; - } - public GeneList() { this.group = GeneListManager.USER_GROUP; } @@ -72,10 +66,6 @@ public String getName() { return name; } - public String getDisplayName() { - return showName ? name : ""; - } - public List getLoci() { return loci; } diff --git a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java index 0994e5f76b..1dfd1d3bce 100644 --- a/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java +++ b/src/main/java/org/broad/igv/sam/AlignmentTrackMenu.java @@ -124,7 +124,7 @@ class AlignmentTrackMenu extends IGVPopupMenu { JMenuItem smallIndelsItem = new JCheckBoxMenuItem("Hide small indels"); smallIndelsItem.setSelected(renderOptions.isHideSmallIndels()); smallIndelsItem.addActionListener(aEvt -> UIUtilities.invokeOnEventThread(() -> { - if(smallIndelsItem.isSelected()) { + if (smallIndelsItem.isSelected()) { String sith = MessageUtils.showInputDialog("Small indel threshold: ", String.valueOf(renderOptions.getSmallIndelThreshold())); try { renderOptions.setSmallIndelThreshold(Integer.parseInt(sith)); @@ -138,7 +138,7 @@ class AlignmentTrackMenu extends IGVPopupMenu { add(smallIndelsItem); // Paired end items - if(dataManager.isPairedEnd()) { + if (dataManager.isPairedEnd()) { addSeparator(); addViewAsPairsMenuItem(); if (clickedAlignment != null) { @@ -180,7 +180,7 @@ class AlignmentTrackMenu extends IGVPopupMenu { } // Sashimi plot - if(alignmentTrack.getExperimentType() == AlignmentTrack.ExperimentType.RNA) { + if (alignmentTrack.getExperimentType() == AlignmentTrack.ExperimentType.RNA) { addSeparator(); JMenuItem sashimi = new JMenuItem("Sashimi Plot"); sashimi.addActionListener(e1 -> SashimiPlot.openSashimiPlot()); @@ -464,15 +464,15 @@ void addGroupMenuItem(final TrackClickEvent te) {//ReferenceFrame frame) { // // if (renderOptions.getGroupByOption() != AlignmentTrack.GroupOption.BASE_AT_POS || oldGroupByPos == null || // !oldGroupByPos.getChr().equals(chrom) || oldGroupByPos.getStart() != chromStart) { // not already sorted by this position - JCheckBoxMenuItem newGroupByPosOption = new JCheckBoxMenuItem("base at " + chrom + - ":" + Globals.DECIMAL_FORMAT.format(1 + chromStart)); - newGroupByPosOption.addActionListener(aEvt -> { - Range groupByPos = new Range(chrom, chromStart, chromStart + 1); - groupAlignments(AlignmentTrack.GroupOption.BASE_AT_POS, null, groupByPos); - }); - groupMenu.add(newGroupByPosOption); - group.add(newGroupByPosOption); - // } + JCheckBoxMenuItem newGroupByPosOption = new JCheckBoxMenuItem("base at " + chrom + + ":" + Globals.DECIMAL_FORMAT.format(1 + chromStart)); + newGroupByPosOption.addActionListener(aEvt -> { + Range groupByPos = new Range(chrom, chromStart, chromStart + 1); + groupAlignments(AlignmentTrack.GroupOption.BASE_AT_POS, null, groupByPos); + }); + groupMenu.add(newGroupByPosOption); + group.add(newGroupByPosOption); + // } JCheckBoxMenuItem newGroupByInsOption = new JCheckBoxMenuItem("insertion at " + chrom + ":" + Globals.DECIMAL_FORMAT.format(1 + chromStart)); @@ -1148,7 +1148,7 @@ private JCheckBoxMenuItem linkedReadItem(String tag) { item.setSelected(!alignmentTrack.isLinkedReadView() && alignmentTrack.isLinkedReads() && tag.equals(renderOptions.getLinkByTag())); item.addActionListener(aEvt -> { boolean linkedReads = item.isSelected(); - if("BX".equals(tag) || "MI".equals(tag)) { + if ("BX".equals(tag) || "MI".equals(tag)) { alignmentTrack.setLinkedReadView(linkedReads, tag); } else { setLinkByTag(linkedReads, tag); @@ -1260,7 +1260,7 @@ private static void addNewLociToFrames(final ReferenceFrame frame, final List loci = createLociList(frame, newLoci); String listName = String.join(" ", loci); // TODO check the trailing " " was unnecessary //Need to sort the frames by position - GeneList geneList = new GeneList(listName, loci, false); + GeneList geneList = new GeneList(listName, loci); geneList.sort(Comparator.comparing(Locus::fromString, SortOption.POSITION_COMPARATOR)); IGV.getInstance().getSession().setCurrentGeneList(geneList); IGV.getInstance().resetFrames(); @@ -1307,7 +1307,7 @@ private static String getLocusStringForAlignment(final ReferenceFrame frame, fin int length = range.getLength(); int start = Math.max(0, adjustedMateStart - length / 2); int end = start + length; - return alignment.getContig() + ":" + start + "-" + end; + return Locus.getFormattedLocusString(alignment.getContig(), start, end); } diff --git a/src/main/java/org/broad/igv/ui/IGV.java b/src/main/java/org/broad/igv/ui/IGV.java index dd00fd6c8d..d078a4cd3e 100644 --- a/src/main/java/org/broad/igv/ui/IGV.java +++ b/src/main/java/org/broad/igv/ui/IGV.java @@ -1050,7 +1050,7 @@ public boolean loadSession(String sessionPath, String locus) { String message = "Error loading session session: " + e.getMessage(); MessageUtils.showMessage(message); recentSessionList.remove(sessionPath); - log.error(message); + log.error(e); return false; } finally { if (inputStream != null) { diff --git a/src/main/java/org/broad/igv/ui/action/SearchCommand.java b/src/main/java/org/broad/igv/ui/action/SearchCommand.java index dfaee9eae7..9bfa25ad1f 100644 --- a/src/main/java/org/broad/igv/ui/action/SearchCommand.java +++ b/src/main/java/org/broad/igv/ui/action/SearchCommand.java @@ -267,7 +267,7 @@ public void showSearchResult(List results) { showMessage = true; } } - GeneList geneList = new GeneList("", loci, false); + GeneList geneList = new GeneList("", loci); IGV.getInstance().getSession().setCurrentGeneList(geneList); } if (resetFrames) { diff --git a/src/main/java/org/broad/igv/ui/commandbar/IGVCommandBar.java b/src/main/java/org/broad/igv/ui/commandbar/IGVCommandBar.java index 7ba6096944..6968c47aab 100644 --- a/src/main/java/org/broad/igv/ui/commandbar/IGVCommandBar.java +++ b/src/main/java/org/broad/igv/ui/commandbar/IGVCommandBar.java @@ -107,7 +107,7 @@ public IGVCommandBar() { chromosomeComboBox.setSelectedItem(currentChr); roiToggleButton.setEnabled(!isWholeGenome); - zoomControl.setEnabled(!isWholeGenome); + zoomControl.setEnabled(!isWholeGenome && !FrameManager.isGeneListMode()); detailsBehaviorButton.addMouseListener(new MouseAdapter() { public void mousePressed(MouseEvent e) { @@ -145,14 +145,9 @@ public ShowDetailsBehavior getDetailsBehavior() { public void setGeneListMode(boolean geneListMode) { -// locationPanel.setEnabled(!geneListMode); chromosomeComboBox.setEnabled(!geneListMode); - if(geneListMode) searchTextField.setText(""); -// searchTextField.setEnabled(!geneListMode); -// goButton.setEnabled(!geneListMode); + if (geneListMode) searchTextField.setText(""); zoomControl.setEnabled(!geneListMode); -// homeButton.setEnabled(true); -// roiToggleButton.setEnabled(!geneListMode); } @@ -184,7 +179,7 @@ public void selectGenome(String genomeId) { public void updateCurrentCoordinates() { - if(IGV.hasInstance()) { + if (IGV.hasInstance()) { String p = ""; ReferenceFrame defaultFrame = FrameManager.getDefaultFrame(); final String chrName = defaultFrame.getChrName(); @@ -200,7 +195,7 @@ public void run() { forwardButton.setEnabled(history.canGoForward()); backButton.setEnabled(history.canGoBack()); roiToggleButton.setEnabled(!Globals.CHR_ALL.equals(chrName)); - zoomControl.setEnabled(!Globals.CHR_ALL.equals(chrName)); + zoomControl.setEnabled(!Globals.CHR_ALL.equals(chrName) && !FrameManager.isGeneListMode()); } }); } @@ -266,7 +261,7 @@ private void homeButtonActionPerformed(java.awt.event.ActionEvent evt) { private void refreshButtonActionPerformed(java.awt.event.ActionEvent evt) { IGVEventBus.getInstance().post(new org.broad.igv.event.RefreshEvent()); - (new ReloadTracksMenuAction("",-1, IGV.getInstance())).actionPerformed(evt); + (new ReloadTracksMenuAction("", -1, IGV.getInstance())).actionPerformed(evt); } @@ -292,7 +287,7 @@ public void receiveEvent(Object e) { if (event.type == ViewChange.Type.ChromosomeChange || event.type == ViewChange.Type.LocusChange) { String chrName = FrameManager.getDefaultFrame().getChrName(); roiToggleButton.setEnabled(!Globals.CHR_ALL.equals(chrName)); - zoomControl.setEnabled(!Globals.CHR_ALL.equals(chrName)); + zoomControl.setEnabled(!Globals.CHR_ALL.equals(chrName) && !FrameManager.isGeneListMode()); if (!chrName.equals(chromosomeComboBox.getSelectedItem())) { chromosomeComboBox.setSelectedItem(chrName); } @@ -306,7 +301,7 @@ public void receiveEvent(Object e) { refreshGenomeListComboBox(); chromosomeComboBox.updateChromosFromGenome(genome); String chrName = FrameManager.getDefaultFrame().getChrName(); - zoomControl.setEnabled(!Globals.CHR_ALL.equals(chrName)); + zoomControl.setEnabled(!Globals.CHR_ALL.equals(chrName) && !FrameManager.isGeneListMode()); } else if (e instanceof GenomeResetEvent) { refreshGenomeListComboBox(); } else { diff --git a/src/main/java/org/broad/igv/ui/panel/CytobandPanel.java b/src/main/java/org/broad/igv/ui/panel/CytobandPanel.java index 6a45dd6459..946e8d5569 100644 --- a/src/main/java/org/broad/igv/ui/panel/CytobandPanel.java +++ b/src/main/java/org/broad/igv/ui/panel/CytobandPanel.java @@ -145,6 +145,7 @@ private void initMouseAdapter() { MouseInputAdapter mouseAdapter = new IGVMouseInputAdapter() { + private final ReferenceFrame referenceFrame = getReferenceFrame(); int lastMousePressX; double viewOrigin; double viewEnd; @@ -159,13 +160,13 @@ public void igvMouseClicked(MouseEvent e) { double newLocation = cytobandScale * mouseX; if (clickCount > 1) { - final int newZoom = getReferenceFrame().getZoom() + 1; - getReferenceFrame().doSetZoomCenter(newZoom, newLocation); + final int newZoom = referenceFrame.getZoom() + 1; + referenceFrame.doSetZoomCenter(newZoom, newLocation); } else { - getReferenceFrame().centerOnLocation(newLocation); + referenceFrame.centerOnLocation(newLocation); } - ViewChange result = ViewChange.Result(); + ViewChange result = ViewChange.LocusChangeResult(referenceFrame.chrName, referenceFrame.origin, referenceFrame.getEnd()); result.setRecordHistory(true); IGVEventBus.getInstance().post(result); @@ -190,12 +191,12 @@ public void mouseReleased(MouseEvent e) { if (isDragging) { WaitCursorManager.CursorToken token = WaitCursorManager.showWaitCursor(); try { - getReferenceFrame().setOrigin(viewOrigin); - getReferenceFrame().recordHistory(); + referenceFrame.setOrigin(viewOrigin); + referenceFrame.recordHistory(); } finally { WaitCursorManager.removeWaitCursor(token); } - ViewChange result = ViewChange.Result(); + ViewChange result = ViewChange.LocusChangeResult(referenceFrame.chrName, referenceFrame.origin, referenceFrame.getEnd()); result.setRecordHistory(true); IGVEventBus.getInstance().post(result); } diff --git a/src/main/java/org/broad/igv/ui/panel/FrameManager.java b/src/main/java/org/broad/igv/ui/panel/FrameManager.java index dd36db8352..df20359821 100644 --- a/src/main/java/org/broad/igv/ui/panel/FrameManager.java +++ b/src/main/java/org/broad/igv/ui/panel/FrameManager.java @@ -350,7 +350,7 @@ public static void addFrames(List newLociStrings) { } //Need to sort the frames by position - GeneList geneList = new GeneList("Current frames", loci, false); + GeneList geneList = new GeneList("Current frames", loci); geneList.sort(FRAME_COMPARATOR); IGV.getInstance().getSession().setCurrentGeneList(geneList); IGV.getInstance().resetFrames(); diff --git a/src/main/java/org/broad/igv/ui/panel/HeaderPanel.java b/src/main/java/org/broad/igv/ui/panel/HeaderPanel.java index ba3df9a2ba..38efadd6c6 100644 --- a/src/main/java/org/broad/igv/ui/panel/HeaderPanel.java +++ b/src/main/java/org/broad/igv/ui/panel/HeaderPanel.java @@ -35,6 +35,9 @@ package org.broad.igv.ui.panel; +import org.broad.igv.event.IGVEventBus; +import org.broad.igv.event.IGVEventObserver; +import org.broad.igv.event.ViewChange; import org.broad.igv.feature.RegionOfInterest; import org.broad.igv.track.TrackMenuUtils; import org.broad.igv.ui.IGV; @@ -58,7 +61,7 @@ * * @author jrobinso */ -public class HeaderPanel extends JPanel implements Transferable, Paintable { +public class HeaderPanel extends JPanel implements Transferable, Paintable, IGVEventObserver { ReferenceFrame frame; private JLabel label; @@ -72,6 +75,7 @@ public class HeaderPanel extends JPanel implements Transferable, Paintable { public HeaderPanel(ReferenceFrame frame) { this.frame = frame; + IGVEventBus.getInstance().subscribe(ViewChange.class, this); init(); } @@ -308,6 +312,16 @@ public int getSnapshotHeight(boolean batch) { return getHeight(); } + @Override + public void receiveEvent(Object event) { + if(label != null && frame != null) label.setText(frame.getFormattedLocusString()); + } + + @Override + public void removeNotify() { + super.removeNotify(); + IGVEventBus.getInstance().unsubscribe(this); + } //private static final Cursor droppableCursor = Cursor.getPredefinedCursor(Cursor.HAND_CURSOR); //private static final Cursor notDroppableCursor = Cursor.getDefaultCursor(); diff --git a/src/main/java/org/broad/igv/ui/panel/HeaderPanelContainer.java b/src/main/java/org/broad/igv/ui/panel/HeaderPanelContainer.java index a0d70f94fe..8bac3d38e2 100644 --- a/src/main/java/org/broad/igv/ui/panel/HeaderPanelContainer.java +++ b/src/main/java/org/broad/igv/ui/panel/HeaderPanelContainer.java @@ -69,6 +69,13 @@ public void setBackground(Color color) { public void createHeaderPanels() { + // Notify child HeaderPanel objects of removal -- its harmless if this gets called twice on a HeaderPanel + for(Component child: getComponents()) { + if(child instanceof HeaderPanel) { + child.removeNotify(); + } + } + removeAll(); Collection frames = FrameManager.getFrames(); contentPanel = new JPanel(); @@ -92,18 +99,6 @@ public void createHeaderPanels() { } add(contentPanel, BorderLayout.CENTER); - // Label for the gene list itself. - if (FrameManager.isGeneListMode()) { - GeneList gl = IGV.getInstance().getSession().getCurrentGeneList(); - String name = gl.getDisplayName(); - if(name != null && name.length() > 0) { - JLabel label = new JLabel(name, JLabel.CENTER); - Border border = BorderFactory.createLineBorder(Color.lightGray); - label.setBorder(border); - add(label, BorderLayout.NORTH); - } - } - invalidate(); } diff --git a/src/main/java/org/broad/igv/ui/panel/ReferenceFrame.java b/src/main/java/org/broad/igv/ui/panel/ReferenceFrame.java index e215a0da93..6cf0fb4a11 100644 --- a/src/main/java/org/broad/igv/ui/panel/ReferenceFrame.java +++ b/src/main/java/org/broad/igv/ui/panel/ReferenceFrame.java @@ -167,7 +167,7 @@ public void setVisible(boolean visible) { public void dragStopped() { setOrigin(Math.round(origin)); // Snap to gride - eventBus.post(ViewChange.Result()); + eventBus.post(ViewChange.LocusChangeResult(chrName, origin, getEnd())); } public void changeGenome(Genome genome) { @@ -186,7 +186,7 @@ public void changeChromosome(String chrName, boolean recordHistory) { public void changeZoom(int newZoom) { doSetZoom(newZoom); - ViewChange result = ViewChange.Result(); + ViewChange result = ViewChange.LocusChangeResult(chrName, origin, getEnd()); result.setRecordHistory(false); eventBus.post(result); } @@ -382,7 +382,7 @@ public void shiftOriginPixels(int delta) { double shiftBP = delta * getScale(); setOrigin(shiftBP + origin); - eventBus.post(ViewChange.Result()); + eventBus.post(ViewChange.LocusChangeResult(chrName, origin, getEnd())); } public void centerOnLocation(String chr, double chrLocation) { diff --git a/src/main/java/org/broad/igv/ui/panel/RegionNavigatorDialog.java b/src/main/java/org/broad/igv/ui/panel/RegionNavigatorDialog.java index d4af81bbfb..da7bc819df 100644 --- a/src/main/java/org/broad/igv/ui/panel/RegionNavigatorDialog.java +++ b/src/main/java/org/broad/igv/ui/panel/RegionNavigatorDialog.java @@ -766,7 +766,7 @@ public void actionPerformed(ActionEvent e) { } loci.add(new RegionOfInterest(roi.getChr(), start, end, roi.getDescription()).getLocusString()); } - GeneList geneList = new GeneList("Regions of Interest", loci, false); + GeneList geneList = new GeneList("Regions of Interest", loci); IGV.getInstance().setGeneList(geneList); IGV.getInstance().resetFrames(); diff --git a/src/test/java/org/broad/igv/ui/panel/ReferenceFrameTest.java b/src/test/java/org/broad/igv/ui/panel/ReferenceFrameTest.java index d2d571a9ab..6689c4ab8d 100644 --- a/src/test/java/org/broad/igv/ui/panel/ReferenceFrameTest.java +++ b/src/test/java/org/broad/igv/ui/panel/ReferenceFrameTest.java @@ -142,7 +142,7 @@ public void testGeneListView(){ int paneSize = 300; List loci = Arrays.asList("chr1:10001-20000", "chr2:30001-40000"); - GeneList geneList = new GeneList("", loci, false); + GeneList geneList = new GeneList("", loci); FrameManager.resetFrames(geneList); List frameList = FrameManager.getFrames(); diff --git a/src/test/java/org/broad/igv/ui/panel/ReferenceFrameTestHeaded.java b/src/test/java/org/broad/igv/ui/panel/ReferenceFrameTestHeaded.java index db4f1c5b4e..a4f5489906 100644 --- a/src/test/java/org/broad/igv/ui/panel/ReferenceFrameTestHeaded.java +++ b/src/test/java/org/broad/igv/ui/panel/ReferenceFrameTestHeaded.java @@ -52,7 +52,7 @@ public void setUp() throws Exception { @Test public void testGeneListView(){ List loci = Arrays.asList("chr1:10000-20000", "chr2:30000-40000"); - GeneList geneList = new GeneList("", loci, false); + GeneList geneList = new GeneList("", loci); IGV.getInstance().getSession().setCurrentGeneList(geneList); IGV.getInstance().resetFrames(); From 89a31951e7534457539276d614676bc1e533769f Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Fri, 1 Sep 2023 14:54:48 -0700 Subject: [PATCH 107/116] Remove deprecated gene lists --- src/main/java/org/broad/igv/lists/GeneListManager.java | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/src/main/java/org/broad/igv/lists/GeneListManager.java b/src/main/java/org/broad/igv/lists/GeneListManager.java index 6be7c8ce3c..4a3612bccd 100644 --- a/src/main/java/org/broad/igv/lists/GeneListManager.java +++ b/src/main/java/org/broad/igv/lists/GeneListManager.java @@ -45,8 +45,7 @@ public class GeneListManager { private static Logger log = LogManager.getLogger(GeneListManager.class); - public static final List DEFAULT_GENE_LISTS = Arrays.asList( - /*"biocarta_cancer_cp.gmt",*/ "examples.gmt", "reactome_cp.gmt", "kegg_cancer_cp.gmt"); + public static final List DEFAULT_GENE_LISTS = Arrays.asList("examples.gmt"); public static final String USER_GROUP = "My lists"; From a060dab4718611169cbe11340834acfb7ff4dfbe Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Fri, 1 Sep 2023 17:19:47 -0700 Subject: [PATCH 108/116] support "displayMode" for json genomes. Fixes #1308 (#1386) support "displayMode" for json genomes. Fixes #1308 --- .../feature/genome/load/JsonGenomeLoader.java | 62 +++++++++++++++---- src/main/java/org/broad/igv/ui/IGV.java | 5 ++ .../org/broad/igv/util/ResourceLocator.java | 11 +++- 3 files changed, 66 insertions(+), 12 deletions(-) diff --git a/src/main/java/org/broad/igv/feature/genome/load/JsonGenomeLoader.java b/src/main/java/org/broad/igv/feature/genome/load/JsonGenomeLoader.java index b8ea20e1f4..61e4db0400 100644 --- a/src/main/java/org/broad/igv/feature/genome/load/JsonGenomeLoader.java +++ b/src/main/java/org/broad/igv/feature/genome/load/JsonGenomeLoader.java @@ -15,6 +15,8 @@ import org.broad.igv.feature.genome.Genome; import org.broad.igv.feature.genome.fasta.FastaBlockCompressedSequence; import org.broad.igv.feature.genome.fasta.FastaIndexedSequence; +import org.broad.igv.track.Track; +import org.broad.igv.track.TrackProperties; import org.broad.igv.track.TribbleFeatureSource; import org.broad.igv.ui.color.ColorUtilities; import org.broad.igv.util.FileUtils; @@ -110,27 +112,56 @@ public Genome loadGenome() throws IOException { res.setFormat(format.getAsString().toLowerCase()); } +// JsonElement colorBy = obj.get("colorBy"); +// if (altColor != null) { +// try { +// properties.colorBy(ColorUtilities.stringToColor(colorBy.toString())); +// } catch (Exception e) { +// log.error("Error parsing color string: " + altColor.toString(), e); +// } +// } + + JsonElement infoURL = obj.get("infoURL"); + if (infoURL != null) { + res.setFeatureInfoURL(infoURL.getAsString()); + } + + + // Track properties + TrackProperties properties = new TrackProperties(); JsonElement color = obj.get("color"); if (color != null) { try { - res.setColor(ColorUtilities.stringToColor(color.toString())); + properties.setColor(ColorUtilities.stringToColor(color.toString())); } catch (Exception e) { - log.error("Error parsing color string: " + color.toString(), e); + log.error("Error parsing color string: " + color, e); + } + } + JsonElement altColor = obj.get("altColor"); + if (altColor != null) { + try { + properties.setAltColor(ColorUtilities.stringToColor(altColor.toString())); + } catch (Exception e) { + log.error("Error parsing color string: " + altColor, e); + } + } + JsonElement displayMode = obj.get("displayMode"); + if (displayMode != null) { + try { + Track.DisplayMode dp = Track.DisplayMode.valueOf(stripQuotes(displayMode.toString())); + properties.setDisplayMode(dp); + } catch (Exception e) { + log.error("Error parsing displayMode " + displayMode, e); } } - JsonElement vizwindow = obj.get("visibilityWindow"); if (vizwindow != null) { - res.setVisibilityWindow(obj.get("visibilityWindow").getAsInt()); + properties.setFeatureVisibilityWindow(obj.get("visibilityWindow").getAsInt()); } else { // If not explicitly set, assume whole chromosome viz window for annotations - res.setVisibilityWindow(-1); - } - - JsonElement infoURL = obj.get("infoURL"); - if (infoURL != null) { - res.setFeatureInfoURL(infoURL.getAsString()); + properties.setFeatureVisibilityWindow(-1); } + res.setTrackProperties(properties); JsonElement indexedElement = obj.get("indexed"); JsonElement hiddenElement = obj.get("hidden"); @@ -139,7 +170,6 @@ public Genome loadGenome() throws IOException { if (indexedElement != null) { res.setIndexed(indexed); } - if (hidden) { if (indexed || trackIndex != null) { log.warn("Hidden tracks cannot be indexed. Ignoring " + trackPath); @@ -149,6 +179,7 @@ public Genome loadGenome() throws IOException { } else { tracks.add(res); } + }); } @@ -267,6 +298,15 @@ private void addToFeatureDB(List locators, Genome genome) { } } + private String stripQuotes(String str) { + if(str.startsWith("\"")) { + return str.substring(1, str.length() - 1); // Assume also ends with + } + else { + return str; + } + } + public static class GenomeDescriptor { String id; String name; diff --git a/src/main/java/org/broad/igv/ui/IGV.java b/src/main/java/org/broad/igv/ui/IGV.java index d078a4cd3e..0517ef27b8 100644 --- a/src/main/java/org/broad/igv/ui/IGV.java +++ b/src/main/java/org/broad/igv/ui/IGV.java @@ -1310,6 +1310,11 @@ public List load(ResourceLocator locator) throws DataLoadException { track.setAttributeValue(Globals.TRACK_NAME_ATTRIBUTE, track.getName()); track.setAttributeValue(Globals.TRACK_DATA_FILE_ATTRIBUTE, fn); track.setAttributeValue(Globals.TRACK_DATA_TYPE_ATTRIBUTE, track.getTrackType().toString()); + + TrackProperties properties = locator.getTrackProperties(); + if(properties != null) { + track.setProperties(properties); + } } } //revalidateTrackPanels(); diff --git a/src/main/java/org/broad/igv/util/ResourceLocator.java b/src/main/java/org/broad/igv/util/ResourceLocator.java index 611c85e540..1d15e59fab 100644 --- a/src/main/java/org/broad/igv/util/ResourceLocator.java +++ b/src/main/java/org/broad/igv/util/ResourceLocator.java @@ -29,6 +29,7 @@ import htsjdk.tribble.Tribble; import org.broad.igv.logging.LogManager; import org.broad.igv.logging.Logger; +import org.broad.igv.track.TrackProperties; import java.awt.*; import java.io.File; @@ -104,12 +105,13 @@ public class ResourceLocator { */ public String format; - /** * A UCSC style track line. Overrides value in file, if any. */ String trackLine; // + TrackProperties trackProperties; + /** * Color for features or data. Somewhat redundant with trackLine. */ @@ -399,6 +401,13 @@ public String getTrackName() { } } + public TrackProperties getTrackProperties() { + return trackProperties; + } + + public void setTrackProperties(TrackProperties trackProperties) { + this.trackProperties = trackProperties; + } public void setName(String name) { this.name = name; From ee5ea52d6231c2b3ddc82dc3e82b9b64d2c4037d Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Fri, 8 Sep 2023 10:22:43 -0700 Subject: [PATCH 109/116] Gvcf (#1389) * Set "relative path" session option to false * Support gvcf files - fixes #1388 --- .../igv/feature/tribble/CodecFactory.java | 1 + .../igv/renderer/IGVFeatureRenderer.java | 6 + .../java/org/broad/igv/renderer/Renderer.java | 2 +- .../broad/igv/session/IGVSessionReader.java | 192 +++--- .../org/broad/igv/track/FeatureTrack.java | 2 +- .../java/org/broad/igv/variant/Variant.java | 6 + .../broad/igv/variant/VariantRenderer.java | 6 +- .../org/broad/igv/variant/VariantTrack.java | 50 +- .../broad/igv/variant/vcf/MateVariant.java | 5 + .../org/broad/igv/variant/vcf/VCFAllele.java | 21 +- .../broad/igv/variant/vcf/VCFGenotype.java | 2 +- .../org/broad/igv/variant/vcf/VCFVariant.java | 44 +- .../org/broad/igv/prefs/preferences.tab | 2 +- test/sessions/filtered_tracks.xml | 590 ++++++++++++++++++ 14 files changed, 783 insertions(+), 146 deletions(-) create mode 100644 test/sessions/filtered_tracks.xml diff --git a/src/main/java/org/broad/igv/feature/tribble/CodecFactory.java b/src/main/java/org/broad/igv/feature/tribble/CodecFactory.java index c51307f339..55f7d43b62 100644 --- a/src/main/java/org/broad/igv/feature/tribble/CodecFactory.java +++ b/src/main/java/org/broad/igv/feature/tribble/CodecFactory.java @@ -86,6 +86,7 @@ public static FeatureCodec getCodec(ResourceLocator locator, Genome genome) { case "vcf4": return new VCFWrapperCodec(new VCFCodec(), genome); case "vcf": + case "gvcf": return new VCFWrapperCodec(getVCFCodec(locator), genome); case "bcf": return new BCF2WrapperCodec(new BCF2Codec(), genome); diff --git a/src/main/java/org/broad/igv/renderer/IGVFeatureRenderer.java b/src/main/java/org/broad/igv/renderer/IGVFeatureRenderer.java index 3aeefd5a20..8b3eb954cd 100644 --- a/src/main/java/org/broad/igv/renderer/IGVFeatureRenderer.java +++ b/src/main/java/org/broad/igv/renderer/IGVFeatureRenderer.java @@ -161,8 +161,14 @@ public void render(List featureList, double virtualPixelStart = (feature.getStart() - origin) / locScale; double virtualPixelEnd = (effectiveEnd - origin) / locScale; + // if pixel width > 5 pixels create gap between variants int pixelStart = (int) Math.round(Math.max(trackRectangleX, virtualPixelStart)); int pixelEnd = (int) Math.round(Math.min(trackRectangleMaxX, virtualPixelEnd)); + if (pixelEnd - pixelStart > 5) { + pixelStart++; + pixelEnd--; + } + final int pixelWidth = pixelEnd - pixelStart; if (isGenotypeRenderer) { diff --git a/src/main/java/org/broad/igv/renderer/Renderer.java b/src/main/java/org/broad/igv/renderer/Renderer.java index 01b81db980..bf286a051d 100644 --- a/src/main/java/org/broad/igv/renderer/Renderer.java +++ b/src/main/java/org/broad/igv/renderer/Renderer.java @@ -41,6 +41,6 @@ */ public interface Renderer { - public abstract void render(java.util.List features, RenderContext context, Rectangle rect, Track track); + void render(java.util.List features, RenderContext context, Rectangle rect, Track track); } diff --git a/src/main/java/org/broad/igv/session/IGVSessionReader.java b/src/main/java/org/broad/igv/session/IGVSessionReader.java index bc450e19c3..7a4f750b0a 100644 --- a/src/main/java/org/broad/igv/session/IGVSessionReader.java +++ b/src/main/java/org/broad/igv/session/IGVSessionReader.java @@ -91,6 +91,11 @@ public class IGVSessionReader implements SessionReader { private int version; private String genomePath; + /** + * There is a single TrackFilter object per session, usually null + */ + TrackFilter filter; + private IGV igv; /** @@ -143,7 +148,6 @@ public List getTracksById(String trackId) { Map fullToRelPathMap = new HashMap(); private Track geneTrack = null; - private Track seqTrack = null; private boolean hasTrackElments; static { @@ -161,7 +165,7 @@ public IGVSessionReader(IGV igv) { /** * @param inputStream * @param session - * @param sessionPath @return + * @param sessionPath -- path to the session file. This can be null * @throws RuntimeException */ @@ -178,9 +182,6 @@ public void loadSession(InputStream inputStream, Session session, String session NodeList trackElements = document.getElementsByTagName("Track"); hasTrackElments = trackElements.getLength() > 0; - HashMap additionalInformation = new HashMap(); - additionalInformation.put(INPUT_FILE_KEY, sessionPath); - // Find the root node, either "session" or "global NodeList nodes = document.getElementsByTagName(SessionElement.SESSION); if (nodes == null || nodes.getLength() == 0) { @@ -189,7 +190,7 @@ public void loadSession(InputStream inputStream, Session session, String session Node rootNode = nodes.item(0); // Recursively process all nodes, starting with the root - processRootNode(session, rootNode, additionalInformation, sessionPath); + processRootNode(session, rootNode, sessionPath); processCombinedDataSourceTracks(); @@ -214,11 +215,10 @@ public void loadSession(InputStream inputStream, Session session, String session * * @param session * @param node - * @param additionalInformation - * @param rootPath + * @param sessionPath */ - private void processRootNode(Session session, Node node, HashMap additionalInformation, String rootPath) { + private void processRootNode(Session session, Node node, String sessionPath) { if ((node == null) || (session == null)) { MessageUtils.showMessage("Invalid session file: root node not found"); @@ -232,7 +232,7 @@ private void processRootNode(Session session, Node node, HashMap additionalInfor } Element element = (Element) node; - process(session, node, additionalInformation, rootPath); + process(session, node, sessionPath); String versionString = getAttribute(element, SessionAttribute.VERSION); try { @@ -246,7 +246,7 @@ private void processRootNode(Session session, Node node, HashMap additionalInfor if (genomeId != null && genomeId.length() > 0) { if (genomeId.equals(GenomeManager.getInstance().getGenomeId())) { - igv.resetSession(rootPath); + igv.resetSession(sessionPath); GenomeManager.getInstance().restoreGenomeTracks(GenomeManager.getInstance().getCurrentGenome()); } else { try { @@ -257,7 +257,7 @@ private void processRootNode(Session session, Node node, HashMap additionalInfor } else { genomePath = genomeId; if (!ParsingUtils.fileExists(genomePath)) { - genomePath = getAbsolutePath(genomeId, rootPath); + genomePath = getAbsolutePath(genomeId, sessionPath); } GenomeManager.getInstance().loadGenome(genomePath, null); } @@ -292,7 +292,7 @@ private void processRootNode(Session session, Node node, HashMap additionalInfor session.setVersion(version); NodeList elements = element.getChildNodes(); - process(session, elements, additionalInformation, rootPath); + process(session, elements, sessionPath); // ReferenceFrame.getInstance().invalidateLocationScale(); } @@ -306,10 +306,8 @@ private void addLeftoverTracks(Collection> tmp) { //For resetting track panels later List> trackPanelAttrs = null; - if (IGV.hasInstance()) { - trackPanelAttrs = IGV.getInstance().getTrackPanelAttrs(); - } - + trackPanelAttrs = igv.getTrackPanelAttrs(); + for (List tracks : tmp) { for (Track track : tracks) { if (track == geneTrack) { @@ -317,7 +315,7 @@ private void addLeftoverTracks(Collection> tmp) { } else if (track.getResourceLocator() != null) { TrackPanel panel = trackPanelCache.get(track.getResourceLocator().getPath()); if (panel == null) { - panel = IGV.getInstance().getPanelFor(track); + panel = igv.getPanelFor(track); trackPanelCache.put(track.getResourceLocator().getPath(), panel); } panel.addTrack(track); @@ -325,9 +323,7 @@ private void addLeftoverTracks(Collection> tmp) { } } - if (IGV.hasInstance()) { - IGV.getInstance().resetPanelHeights(trackPanelAttrs.get(0), trackPanelAttrs.get(1)); - } + igv.resetPanelHeights(trackPanelAttrs.get(0), trackPanelAttrs.get(1)); } } @@ -338,7 +334,7 @@ private void addLeftoverTracks(Collection> tmp) { * @param session * @param element */ - private void process(Session session, Node element, HashMap additionalInformation, String rootPath) { + private void process(Session session, Node element, String sessionPath) { if ((element == null) || (session == null)) { return; @@ -347,47 +343,47 @@ private void process(Session session, Node element, HashMap additionalInformatio String nodeName = element.getNodeName(); if (nodeName.equalsIgnoreCase(SessionElement.RESOURCES) || nodeName.equalsIgnoreCase(SessionElement.FILES)) { - processResources(session, (Element) element, additionalInformation, rootPath); + processResources(session, (Element) element, sessionPath); } else if (nodeName.equalsIgnoreCase(SessionElement.RESOURCE) || nodeName.equalsIgnoreCase(SessionElement.DATA_FILE)) { - processResource(session, (Element) element, additionalInformation, rootPath); + processResource(session, (Element) element, sessionPath); } else if (nodeName.equalsIgnoreCase(SessionElement.REGIONS)) { - processRegions(session, (Element) element, additionalInformation, rootPath); + processRegions(session, (Element) element, sessionPath); } else if (nodeName.equalsIgnoreCase(SessionElement.REGION)) { - processRegion(session, (Element) element, additionalInformation, rootPath); + processRegion(session, (Element) element, sessionPath); } else if (nodeName.equalsIgnoreCase(SessionElement.GENE_LIST)) { - processGeneList(session, (Element) element, additionalInformation); + processGeneList(session, (Element) element); } else if (nodeName.equalsIgnoreCase(SessionElement.FILTER)) { - processFilter(session, (Element) element, additionalInformation, rootPath); + processFilter(session, (Element) element, sessionPath); } else if (nodeName.equalsIgnoreCase(SessionElement.FILTER_ELEMENT)) { - processFilterElement(session, (Element) element, additionalInformation, rootPath); + processFilterElement(session, (Element) element, sessionPath); } else if (nodeName.equalsIgnoreCase(SessionElement.COLOR_SCALES)) { - processColorScales(session, (Element) element, additionalInformation, rootPath); + processColorScales(session, (Element) element, sessionPath); } else if (nodeName.equalsIgnoreCase(SessionElement.COLOR_SCALE)) { - processColorScale(session, (Element) element, additionalInformation, rootPath); + processColorScale(session, (Element) element, sessionPath); } else if (nodeName.equalsIgnoreCase(SessionElement.PREFERENCES)) { - processPreferences(session, (Element) element, additionalInformation); + processPreferences(session, (Element) element); } else if (nodeName.equalsIgnoreCase(SessionElement.DATA_TRACKS) || nodeName.equalsIgnoreCase(SessionElement.FEATURE_TRACKS) || nodeName.equalsIgnoreCase(SessionElement.PANEL)) { - processPanel(session, (Element) element, additionalInformation, rootPath); + processPanel(session, (Element) element, sessionPath); } else if (nodeName.equalsIgnoreCase(SessionElement.PANEL_LAYOUT)) { - processPanelLayout(session, (Element) element, additionalInformation); + processPanelLayout(session, (Element) element); } else if (nodeName.equalsIgnoreCase(SessionElement.HIDDEN_ATTRIBUTES)) { - processHiddenAttributes(session, (Element) element, additionalInformation); + processHiddenAttributes(session, (Element) element); } else if (nodeName.equalsIgnoreCase(SessionElement.VISIBLE_ATTRIBUTES)) { - processVisibleAttributes(session, (Element) element, additionalInformation); + processVisibleAttributes(session, (Element) element); } } - private void processResources(Session session, Element element, HashMap additionalInformation, String rootPath) { + private void processResources(Session session, Element element, String sessionPath) { dataFiles = new ArrayList(); missingDataFiles = new ArrayList(); NodeList elements = element.getChildNodes(); - process(session, elements, additionalInformation, rootPath); + process(session, elements, sessionPath); if (missingDataFiles.size() > 0) { StringBuffer message = new StringBuffer(); @@ -521,9 +517,8 @@ private void processResources(Session session, Element element, HashMap addition * * @param session * @param element - * @param additionalInformation */ - void processResource(Session session, Element element, HashMap additionalInformation, String rootPath) { + void processResource(Session session, Element element, String sessionPath) { String nodeName = element.getNodeName(); boolean oldSession = nodeName.equals(SessionElement.DATA_FILE); @@ -539,12 +534,12 @@ void processResource(Session session, Element element, HashMap additionalInforma String colorString = getAttribute(element, SessionAttribute.COLOR); String index = getAttribute(element, SessionAttribute.INDEX); - // URL to a database or webservice + // URL to a database or webservice -- this is not common String serverURL = getAttribute(element, SessionAttribute.SERVER_URL); - - // Older sessions used the "name" attribute for the path. + String path = getAttribute(element, SessionAttribute.PATH); + // Older sessions used the "name" attribute for the path. if (oldSession && name != null) { path = name; int idx = name.lastIndexOf("/"); @@ -553,9 +548,9 @@ void processResource(Session session, Element element, HashMap additionalInforma } } - String absolutePath = (rootPath == null || "ga4gh".equals(type) || ParsingUtils.isDataURL(path)) ? + String absolutePath = (sessionPath == null || ParsingUtils.isDataURL(path)) ? path : - getAbsolutePath(path, rootPath); + getAbsolutePath(path, sessionPath); fullToRelPathMap.put(absolutePath, path); @@ -564,12 +559,12 @@ void processResource(Session session, Element element, HashMap additionalInforma if (index != null) resourceLocator.setIndexPath(index); if (coverage != null) { - String absoluteCoveragePath = coverage.equals(".") ? coverage : getAbsolutePath(coverage, rootPath); + String absoluteCoveragePath = coverage.equals(".") ? coverage : getAbsolutePath(coverage, sessionPath); resourceLocator.setCoverage(absoluteCoveragePath); } if (mapping != null) { - String absoluteMappingPath = mapping.equals(".") ? mapping : getAbsolutePath(mapping, rootPath); + String absoluteMappingPath = mapping.equals(".") ? mapping : getAbsolutePath(mapping, sessionPath); resourceLocator.setMappingPath(absoluteMappingPath); } @@ -617,18 +612,18 @@ void processResource(Session session, Element element, HashMap additionalInforma NodeList elements = element.getChildNodes(); - process(session, elements, additionalInformation, rootPath); + process(session, elements, sessionPath); } - private void processRegions(Session session, Element element, HashMap additionalInformation, String rootPath) { + private void processRegions(Session session, Element element, String sessionPath) { session.clearRegionsOfInterest(); NodeList elements = element.getChildNodes(); - process(session, elements, additionalInformation, rootPath); + process(session, elements, sessionPath); } - private void processRegion(Session session, Element element, HashMap additionalInformation, String rootPath) { + private void processRegion(Session session, Element element, String sessionPath) { String chromosome = getAttribute(element, SessionAttribute.CHROMOSOME); String start = getAttribute(element, SessionAttribute.START_INDEX); @@ -636,13 +631,13 @@ private void processRegion(Session session, Element element, HashMap additionalI String description = getAttribute(element, SessionAttribute.DESCRIPTION); RegionOfInterest region = new RegionOfInterest(chromosome, new Integer(start), new Integer(end), description); - IGV.getInstance().addRegionOfInterest(region); + igv.addRegionOfInterest(region); NodeList elements = element.getChildNodes(); - process(session, elements, additionalInformation, rootPath); + process(session, elements, sessionPath); } - private void processHiddenAttributes(Session session, Element element, HashMap additionalInformation) { + private void processHiddenAttributes(Session session, Element element) { NodeList elements = element.getChildNodes(); Set attributes = new HashSet(); for (int i = 0; i < elements.getLength(); i++) { @@ -660,9 +655,8 @@ private void processHiddenAttributes(Session session, Element element, HashMap a * * @param session * @param element - * @param additionalInformation */ - private void processVisibleAttributes(Session session, Element element, HashMap additionalInformation) { + private void processVisibleAttributes(Session session, Element element) { NodeList elements = element.getChildNodes(); if (elements.getLength() > 0) { @@ -682,7 +676,7 @@ private void processVisibleAttributes(Session session, Element element, HashMap } } - private void processGeneList(Session session, Element element, HashMap additionalInformation) { + private void processGeneList(Session session, Element element) { String name = getAttribute(element, SessionAttribute.NAME); @@ -734,42 +728,50 @@ private void processFrames(Element element) { FrameManager.setFrames(reorderedFrames); } } - IGV.getInstance().resetFrames(); + igv.resetFrames(); } - private void processFilter(Session session, Element element, HashMap additionalInformation, String rootPath) { + /** + * Example + * + * @param session + * @param element + * @param sessionPath + */ + private void processFilter(Session session, Element element, String sessionPath) { String match = getAttribute(element, SessionAttribute.FILTER_MATCH); String showAllTracks = getAttribute(element, SessionAttribute.FILTER_SHOW_ALL_TRACKS); String filterName = getAttribute(element, SessionAttribute.NAME); - TrackFilter filter = new TrackFilter(filterName, null); - additionalInformation.put(SessionElement.FILTER, filter); + filter = new TrackFilter(filterName, null); NodeList elements = element.getChildNodes(); - process(session, elements, additionalInformation, rootPath); + process(session, elements, sessionPath); // Save the filter session.setFilter(filter); - // Set filter properties + // Update UI elements -- the matchAll and showAll state is kept in the UI, not the Filter object. This seems wrong. TODO if ("all".equalsIgnoreCase(match)) { - IGV.getInstance().setFilterMatchAll(true); + igv.setFilterMatchAll(true); } else if ("any".equalsIgnoreCase(match)) { - IGV.getInstance().setFilterMatchAll(false); + igv.setFilterMatchAll(false); } - if ("true".equalsIgnoreCase(showAllTracks)) { - IGV.getInstance().setFilterShowAllTracks(true); + igv.setFilterShowAllTracks(true); } else { - IGV.getInstance().setFilterShowAllTracks(false); + igv.setFilterShowAllTracks(false); } } private void processFilterElement(Session session, Element element, - HashMap additionalInformation, String rootPath) { + String sessionPath) { + + if (filter == null) { + throw new RuntimeException("Filter elements defined before filter"); + } - TrackFilter filter = (TrackFilter) additionalInformation.get(SessionElement.FILTER); String item = getAttribute(element, SessionAttribute.ITEM); String operator = getAttribute(element, SessionAttribute.OPERATOR); String value = getAttribute(element, SessionAttribute.VALUE); @@ -782,7 +784,7 @@ private void processFilterElement(Session session, Element element, filter.add(trackFilterElement); NodeList elements = element.getChildNodes(); - process(session, elements, additionalInformation, rootPath); + process(session, elements, sessionPath); } /** @@ -800,7 +802,7 @@ private static boolean nodeIsTrack(Node node) { node.getNodeName().equalsIgnoreCase(SessionElement.TRACK)); } - private void processPanel(Session session, Element element, HashMap additionalInformation, String rootPath) { + private void processPanel(Session session, Element element, String sessionPath) { if (panelElementPresent == false) { // First panel to be processed, do this only once. @@ -829,12 +831,12 @@ private void processPanel(Session session, Element element, HashMap additionalIn for (int i = 0; i < elements.getLength(); i++) { Node childNode = elements.item(i); if (nodeIsTrack(childNode)) { - List tracks = processTrack(session, (Element) childNode, additionalInformation, rootPath); + List tracks = processTrack(session, (Element) childNode, sessionPath); if (tracks != null) { panelTracks.addAll(tracks); } } else { - process(session, childNode, additionalInformation, rootPath); + process(session, childNode, sessionPath); } } @@ -850,11 +852,11 @@ private void processPanel(Session session, Element element, HashMap additionalIn } } - TrackPanel panel = IGV.getInstance().getTrackPanel(panelName); + TrackPanel panel = igv.getTrackPanel(panelName); panel.addTracks(panelTracks); } - private void processPanelLayout(Session session, Element element, HashMap additionalInformation) { + private void processPanelLayout(Session session, Element element) { String nodeName = element.getNodeName(); @@ -885,11 +887,10 @@ private void processPanelLayout(Session session, Element element, HashMap additi * * @param session * @param element - * @param additionalInformation * @return */ - private List processTrack(Session session, Element element, HashMap additionalInformation, String rootPath) { + private List processTrack(Session session, Element element, String sessionPath) { String id = getAttribute(element, SessionAttribute.ID); @@ -900,7 +901,7 @@ private List processTrack(Session session, Element element, HashMap addit if (matchedTracks == null) { //Try creating an absolute path for the id if (id != null) { - matchedTracks = allTracks.get(getAbsolutePath(id, rootPath)); + matchedTracks = allTracks.get(getAbsolutePath(id, sessionPath)); } } @@ -915,7 +916,7 @@ private List processTrack(Session session, Element element, HashMap addit // reference sequence, combined, and merged tracks. // Check for errors during load -// String absPath = getAbsolutePath(id, rootPath); +// String absPath = getAbsolutePath(id, sessionPath); // if (erroredResources.contains(id) || erroredResources.contains(absPath)) { // return Collections.emptyList(); // } @@ -941,7 +942,7 @@ private List processTrack(Session session, Element element, HashMap addit } if (className.contains("MergedTracks")) { - List memberTracks = processChildTracks(session, element, additionalInformation, rootPath); + List memberTracks = processChildTracks(session, element, sessionPath); ((MergedTracks) track).setMemberTracks(memberTracks); NodeList nodeList = element.getElementsByTagName("DataRange"); @@ -966,7 +967,7 @@ private List processTrack(Session session, Element element, HashMap addit //} NodeList elements = element.getChildNodes(); - process(session, elements, additionalInformation, rootPath); + process(session, elements, sessionPath); return matchedTracks; } @@ -977,18 +978,17 @@ private List processTrack(Session session, Element element, HashMap addit * * @param session * @param element - * @param additionalInformation - * @param rootPath + * @param sessionPath * @return List of processed tracks. */ - private List processChildTracks(Session session, Element element, HashMap additionalInformation, String rootPath) { + private List processChildTracks(Session session, Element element, String sessionPath) { NodeList memberTrackNodes = element.getChildNodes(); List memberTracks = new ArrayList<>(memberTrackNodes.getLength()); for (int index = 0; index < memberTrackNodes.getLength(); index++) { Node memberNode = memberTrackNodes.item(index); if (nodeIsTrack(memberNode)) { - List addedTracks = processTrack(session, (Element) memberNode, additionalInformation, rootPath); + List addedTracks = processTrack(session, (Element) memberNode, sessionPath); if (addedTracks != null) { for (Track t : addedTracks) { if (t instanceof DataTrack) { @@ -1045,13 +1045,13 @@ private void processCombinedDataSourceTracks() { } - private void processColorScales(Session session, Element element, HashMap additionalInformation, String rootPath) { + private void processColorScales(Session session, Element element, String sessionPath) { NodeList elements = element.getChildNodes(); - process(session, elements, additionalInformation, rootPath); + process(session, elements, sessionPath); } - private void processColorScale(Session session, Element element, HashMap additionalInformation, String rootPath) { + private void processColorScale(Session session, Element element, String sessionPath) { String trackType = getAttribute(element, SessionAttribute.TYPE); String value = getAttribute(element, SessionAttribute.VALUE); @@ -1059,10 +1059,10 @@ private void processColorScale(Session session, Element element, HashMap additio setColorScaleSet(session, trackType, value); NodeList elements = element.getChildNodes(); - process(session, elements, additionalInformation, rootPath); + process(session, elements, sessionPath); } - private void processPreferences(Session session, Element element, HashMap additionalInformation) { + private void processPreferences(Session session, Element element) { NodeList elements = element.getChildNodes(); for (int i = 0; i < elements.getLength(); i++) { @@ -1083,10 +1083,10 @@ private void processPreferences(Session session, Element element, HashMap additi * @param session * @param elements */ - private void process(Session session, NodeList elements, HashMap additionalInformation, String rootPath) { + private void process(Session session, NodeList elements, String sessionPath) { for (int i = 0; i < elements.getLength(); i++) { Node childNode = elements.item(i); - process(session, childNode, additionalInformation, rootPath); + process(session, childNode, sessionPath); } } @@ -1227,10 +1227,10 @@ private void removeAllTracksFromPanels() { } } - private String getAbsolutePath(String path, String rootPath) { - String absolute = FileUtils.getAbsolutePath(path, rootPath); + private String getAbsolutePath(String path, String sessionPath) { + String absolute = FileUtils.getAbsolutePath(path, sessionPath); if (!(new File(absolute)).exists() && path.startsWith("~")) { - String tmp = FileUtils.getAbsolutePath(path.replaceFirst("~", System.getProperty("user.home")), rootPath); + String tmp = FileUtils.getAbsolutePath(path.replaceFirst("~", System.getProperty("user.home")), sessionPath); if ((new File(tmp)).exists()) { absolute = tmp; } diff --git a/src/main/java/org/broad/igv/track/FeatureTrack.java b/src/main/java/org/broad/igv/track/FeatureTrack.java index 35865fbfa8..09024285ac 100644 --- a/src/main/java/org/broad/igv/track/FeatureTrack.java +++ b/src/main/java/org/broad/igv/track/FeatureTrack.java @@ -73,7 +73,7 @@ public class FeatureTrack extends AbstractTrack implements IGVEventObserver { private static Logger log = LogManager.getLogger(FeatureTrack.class); - public static final int MINIMUM_FEATURE_SPACING = 5; + public static final int MINIMUM_FEATURE_SPACING = 0; public static final int DEFAULT_MARGIN = 5; public static final int NO_FEATURE_ROW_SELECTED = -1; protected static final Color SELECTED_FEATURE_ROW_COLOR = new Color(100, 100, 100, 30); diff --git a/src/main/java/org/broad/igv/variant/Variant.java b/src/main/java/org/broad/igv/variant/Variant.java index c16e803b18..a176782e94 100644 --- a/src/main/java/org/broad/igv/variant/Variant.java +++ b/src/main/java/org/broad/igv/variant/Variant.java @@ -53,6 +53,12 @@ public interface Variant extends Feature { */ String getType(); + /** + * @return true if all alleles are NON_REF. This is common with gvcf files + * @return + */ + boolean isNonRef(); + /** * @return return true if the variant has been marked as filtered diff --git a/src/main/java/org/broad/igv/variant/VariantRenderer.java b/src/main/java/org/broad/igv/variant/VariantRenderer.java index c5192715c9..d52d50bb7d 100644 --- a/src/main/java/org/broad/igv/variant/VariantRenderer.java +++ b/src/main/java/org/broad/igv/variant/VariantRenderer.java @@ -85,6 +85,8 @@ public class VariantRenderer { //extends FeatureRenderer { private Color colorNoCall; private Color colorNoCallAlpha; + private Color nonRefColor = new Color(200, 200, 215); + public VariantRenderer(VariantTrack track) { this.track = track; updateColors(); @@ -148,7 +150,9 @@ public void renderSiteBand(Variant variant, final Color refColor; double percent; - Color colorAlleleRef = track.getColor(); + Color colorAlleleRef = variant.isNonRef() ? nonRefColor : track.getColor(); + //colorAlleleRef = new Color(((int)(Math.random()*255)),((int)(Math.random()*255)),((int)(Math.random()*255))); + Color colorAlleleRefAlpha = useAlpha ? ColorUtilities.getCompositeColor(colorAlleleRef, alphaValue) : colorAlleleRef; if(track.getSiteColorMode() == VariantTrack.ColorMode.NONE) { diff --git a/src/main/java/org/broad/igv/variant/VariantTrack.java b/src/main/java/org/broad/igv/variant/VariantTrack.java index b3e12f643b..97910f08d7 100644 --- a/src/main/java/org/broad/igv/variant/VariantTrack.java +++ b/src/main/java/org/broad/igv/variant/VariantTrack.java @@ -96,7 +96,8 @@ public static boolean isVCF(String format) { return (format.equals("vcf3") || format.equals("vcf4") || format.equals("vcf") || - format.equals("bcf")); + format.equals("bcf") || + format.equals("gvcf")); } @@ -236,12 +237,11 @@ public VariantTrack(ResourceLocator locator, FeatureSource source, List boolean bypassFileAutoDiscovery = prefMgr.getAsBoolean(BYPASS_FILE_AUTO_DISCOVERY); if (vcfToBamMapping == null && path != null && !bypassFileAutoDiscovery) { - String mappingFile = ""; + String mappingFile = ""; int queryStart = path.indexOf("?"); - if (queryStart > -1) - { - String query = path.substring(queryStart); - path = path.substring(0,queryStart); + if (queryStart > -1) { + String query = path.substring(queryStart); + path = path.substring(0, queryStart); mappingFile = path + ".mapping" + query; } @@ -349,7 +349,7 @@ private void setupGroupsFromAttributes() { return; } - if(allSamples != null) { + if (allSamples != null) { for (String sample : allSamples) { String sampleGroup = manager.getAttribute(sample, newGroupByAttribute); @@ -373,7 +373,7 @@ private void setupGroupsFromAttributes() { * @param comparator the comparator to sort by */ public void sortSamples(Comparator comparator) { - if(allSamples != null) { + if (allSamples != null) { Collections.sort(allSamples, comparator); for (List samples : samplesByGroups.values()) { Collections.sort(samples, comparator); @@ -544,12 +544,10 @@ protected void renderFeatureImpl(RenderContext context, Rectangle trackRectangle x--; } - //Make sure we have some whitespace between this - //feature and the previous one, but only if they don't - //actually overlap and the current size is reasonably large - int spacing = x - lastEndX; - if (spacing > 0 && spacing < minSpacing && w > 2 * minSpacing) { - x += minSpacing - spacing; + // if pixel width > 5 pixels create gap between variants + if (w > 5) { + x++; + w -= 2; } tmpRect.y = curRowTop; @@ -1045,13 +1043,16 @@ private String getVariantToolTip(Variant variant) { String id = variant.getID(); StringBuffer toolTip = new StringBuffer(); + if(id.length() > 0) { + toolTip.append("ID: " + id + "
        "); + } toolTip.append("Chr: " + variant.getChr()); toolTip.append("
        Position: " + variant.getPositionString()); - toolTip.append("
        ID: " + id); toolTip.append("
        Reference: " + variant.getReference()); List alternates = variant.getAlternateAlleles(); String alternateString = null; if (alternates.size() > 0) { + String tmp = alternates.get(0).toString(); alternateString = StringUtils.join(alternates, ","); toolTip.append("
        Alternate: " + alternateString); } @@ -1088,12 +1089,21 @@ private String getVariantToolTip(Variant variant) { } double[] af = variant.getAlleleFreqs(); - String afString = af.length > 1 ? "
        Allele Fequencies: " : "
        Allele Frequency: "; - for (int i = 0; i < af.length; i++) { - afString += Double.toString(af[i]); - if (i < af.length - 1) afString += ", "; + + int nonNegativeCounts=0; + for(int i=0; i= 0) nonNegativeCounts++; + } + if(nonNegativeCounts > 0) { + String afString = nonNegativeCounts > 1 ? "
        Allele Fequencies: " : "
        Allele Frequency: "; + for (int i = 0; i < af.length; i++) { + if(af[i] >= 0) { + afString += Double.toString(af[i]); + if (i < af.length - 1) afString += ", "; + } + } + toolTip.append(afString); } - toolTip.append(afString); } if (variant.getAttributes().size() > 0) { toolTip.append(getVariantInfo(variant)); diff --git a/src/main/java/org/broad/igv/variant/vcf/MateVariant.java b/src/main/java/org/broad/igv/variant/vcf/MateVariant.java index a9106e7f90..774ba9c765 100644 --- a/src/main/java/org/broad/igv/variant/vcf/MateVariant.java +++ b/src/main/java/org/broad/igv/variant/vcf/MateVariant.java @@ -54,6 +54,11 @@ public String getType() { return mate.getType(); } + @Override + public boolean isNonRef() { + return mate.isNonRef(); + } + @Override public boolean isFiltered() { return mate.isFiltered(); diff --git a/src/main/java/org/broad/igv/variant/vcf/VCFAllele.java b/src/main/java/org/broad/igv/variant/vcf/VCFAllele.java index 5187bdd232..d5126ba3a1 100644 --- a/src/main/java/org/broad/igv/variant/vcf/VCFAllele.java +++ b/src/main/java/org/broad/igv/variant/vcf/VCFAllele.java @@ -33,22 +33,31 @@ */ public class VCFAllele implements Allele { - byte[] bases; + htsjdk.variant.variantcontext.Allele htsjdkAllele; - public VCFAllele(byte[] bases) { - this.bases = bases; + + public VCFAllele(htsjdk.variant.variantcontext.Allele htsjdkAllele) { + this.htsjdkAllele = htsjdkAllele; } public byte[] getBases() { - return bases; + return htsjdkAllele.getBases(); + } + + public boolean isNonRefAllele() { + return htsjdkAllele.isNonRefAllele(); } @Override public String getDisplayString() { - return new String(bases); + return toString(); } public String toString() { - return new String(bases); + if (htsjdkAllele.isNonRefAllele()) { + return "NON_REF"; + } else + return htsjdkAllele.getDisplayString(); } + } diff --git a/src/main/java/org/broad/igv/variant/vcf/VCFGenotype.java b/src/main/java/org/broad/igv/variant/vcf/VCFGenotype.java index d5276e7ace..6fee243a00 100644 --- a/src/main/java/org/broad/igv/variant/vcf/VCFGenotype.java +++ b/src/main/java/org/broad/igv/variant/vcf/VCFGenotype.java @@ -125,7 +125,7 @@ public List getAlleles() { List tmp = vcfGenotype.getAlleles(); if (tmp != null) { for (Allele a : tmp) { - alleles.add(new VCFAllele(a.getBases())); + alleles.add(new VCFAllele(a)); } } } diff --git a/src/main/java/org/broad/igv/variant/vcf/VCFVariant.java b/src/main/java/org/broad/igv/variant/vcf/VCFVariant.java index 7971ff8db4..182c033067 100644 --- a/src/main/java/org/broad/igv/variant/vcf/VCFVariant.java +++ b/src/main/java/org/broad/igv/variant/vcf/VCFVariant.java @@ -35,6 +35,7 @@ import htsjdk.variant.variantcontext.VariantContext; import htsjdk.variant.variantcontext.VariantContextBuilder; +import java.text.DecimalFormat; import java.util.*; /** @@ -45,6 +46,8 @@ public class VCFVariant implements Variant { private static Logger log = LogManager.getLogger(Variant.class); + static final DecimalFormat numFormat = new DecimalFormat("###,###,###"); + VariantContext variantContext; List alternateAlleles; // private ZygosityCount zygosityCount; @@ -60,6 +63,8 @@ public class VCFVariant implements Variant { private int start = -1; private int totalAlleleCount = 0; + private boolean nonRef; + public VCFVariant(VariantContext variantContext, String chr) { this.variantContext = variantContext; this.chr = chr; @@ -115,9 +120,19 @@ private void init() { } } + // The variant is considered "NON_REF" if all of the alternate alleles are non-ref + for(htsjdk.variant.variantcontext.Allele a : variantContext.getAlternateAlleles()){ + if(a.isNonRefAllele()) { + nonRef = true; + } else { + nonRef = false; + break; + } + } } + /** * Allele frequency is a comma separated list of doubles * We strip away brackets and parentheses @@ -190,7 +205,7 @@ public List getAlternateAlleles() { List tmp = variantContext.getAlternateAlleles(); alternateAlleles = new ArrayList(tmp.size()); for (htsjdk.variant.variantcontext.Allele a : tmp) { - alternateAlleles.add(new VCFAllele(a.getBases())); + alternateAlleles.add(new VCFAllele(a)); } } return alternateAlleles; @@ -210,6 +225,10 @@ public String getType() { } + public boolean isNonRef() { + return nonRef; + } + /** * Return the allele frequency as annotated with an AF or GMAF attribute. A value of -1 indicates * no annotation (unknown allele frequency). @@ -331,14 +350,16 @@ public String toString() { @Override public String getPositionString() { if (variantContext.getStart() == variantContext.getEnd()) { - return String.valueOf(variantContext.getStart()); + return numFormat.format(variantContext.getStart()); } else { String chr2 = variantContext.getAttributeAsString("CHR2", null); if(chr2 == null || chr2.equals(getChr())) { - return String.format("%d-%d", variantContext.getStart(), variantContext.getEnd()); + return numFormat.format( variantContext.getStart()) + "-" + numFormat.format(variantContext.getEnd()); } else { - return String.format("%s:%d-%s:%d", getChr(), variantContext.getStart(), chr2, variantContext.getEnd()); + // TODO -- I'm not sure this is correct + return getChr() + ":" + numFormat.format(variantContext.getStart()) + " " + + chr2 + ":" + numFormat.format(variantContext.getEnd()); } } @@ -353,21 +374,6 @@ public VariantContext getVariantContext() { return variantContext; } - public static VariantContext getVariantContext(Variant variant) { - if (variant instanceof VCFVariant) { - return ((VCFVariant) variant).getVariantContext(); - } else { - List alleleList = new ArrayList(variant.getAlternateAlleles().size() + 1); - alleleList.add(htsjdk.variant.variantcontext.Allele.create(variant.getReference(), true)); - for (Allele all : variant.getAlternateAlleles()) { - alleleList.add(htsjdk.variant.variantcontext.Allele.create(all.getBases(), false)); - } - VariantContextBuilder vcb = new VariantContextBuilder(variant.getID(), variant.getChr(), variant.getStart(), variant.getEnd(), alleleList); - return vcb.make(); - } - } - - /** * VCFs specify padding bases at the beginning of indels so they can be positioned properly. * We display the variant only where it actually differs from the reference. So we find the longest diff --git a/src/main/resources/org/broad/igv/prefs/preferences.tab b/src/main/resources/org/broad/igv/prefs/preferences.tab index 68f316b430..d861cbab75 100644 --- a/src/main/resources/org/broad/igv/prefs/preferences.tab +++ b/src/main/resources/org/broad/igv/prefs/preferences.tab @@ -1,5 +1,5 @@ #General -SESSION.RELATIVE_PATH Use relative paths in session files boolean TRUE +SESSION.RELATIVE_PATH Use relative paths in session files boolean FALSE IGV.single.track.pane Display all tracks in a single panel boolean FALSE IGV.track.show.attribute.views Show attribute panel boolean FALSE SHOW_DEFAULT_TRACK_ATTRIBUTES Show default track attributes boolean FALSE diff --git a/test/sessions/filtered_tracks.xml b/test/sessions/filtered_tracks.xml new file mode 100644 index 0000000000..92753365c8 --- /dev/null +++ b/test/sessions/filtered_tracks.xml @@ -0,0 +1,590 @@ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + From eb1a0c3a8d49e3e3fc4b094272a2bb1538ab5a89 Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Tue, 12 Sep 2023 16:09:28 -0700 Subject: [PATCH 110/116] OAuth refactoring (#1390) * Defer initializing default Google oAuth until needed. * Support multiple oAuth configurations as an array in oauth-config.json. e.g. an Azure and Amazon config --- .gitignore | 1 + examples/oauth/oauth-config-amazon.json | 11 +-- examples/oauth/oauth-config-google.json | 10 +- examples/oauth/oauth-config-microsoft.json | 12 +-- .../org/broad/igv/oauth/OAuthProvider.java | 42 ++------- .../org/broad/igv/oauth/OAuthURLForm.java | 60 ++++++++++++ .../java/org/broad/igv/oauth/OAuthUtils.java | 92 ++++++++++++------- .../org/broad/igv/prefs/IGVPreferences.java | 15 ++- src/main/java/org/broad/igv/ui/IGV.java | 8 -- .../java/org/broad/igv/ui/IGVMenuBar.java | 66 +++++++------ .../java/org/broad/igv/ui/UIConstants.java | 2 +- .../igv/ui/action/LoadFromURLMenuAction.java | 12 --- .../java/org/broad/igv/util/HttpUtils.java | 4 +- 13 files changed, 199 insertions(+), 136 deletions(-) create mode 100644 src/main/java/org/broad/igv/oauth/OAuthURLForm.java diff --git a/.gitignore b/.gitignore index 4c11921a08..fc6433d38e 100644 --- a/.gitignore +++ b/.gitignore @@ -22,3 +22,4 @@ out/ /test/batch/snapshots/ /src/main/java/org/broad/igv/sam/mods/BMScale.java /src/main/java/org/broad/igv/sam/mods/BMScale.form +/ignore/ diff --git a/examples/oauth/oauth-config-amazon.json b/examples/oauth/oauth-config-amazon.json index da133eb5d6..410cd9022c 100644 --- a/examples/oauth/oauth-config-amazon.json +++ b/examples/oauth/oauth-config-amazon.json @@ -1,16 +1,15 @@ { - "client_id": "3f4ujenfmr77tg12idlkebpkoh", - "client_secret": "en1q6638m4dogrr6erhrmitim67sjilc6htjnfmf6ljk2q3j9og", + "apiKey": "", + "project_id": "igv", "auth_provider": "Amazon", "auth_provider_x509_cert_url": "https://www.googleapis.com/oauth2/v1/certs", "aws_region": "ap-southeast-2", "scope": "email%20openid%20profile", - "redirect_uris": [ - "http://localhost:60151/oauthCallback" - ], + "client_id": "3f4ujenfmr77tg12iofbebpkoh", + "client_secret": "en1q6638m4dogrr6erhosetim67sjilc6htjnfmf6ljk2q3j9og", "authorization_endpoint": "https://igv-demo.auth.ap-southeast-2.amazoncognito.com/login", "token_endpoint": "https://igv-demo.auth.ap-southeast-2.amazoncognito.com/token", "aws_cognito_fed_pool_id": "ap-southeast-2:15b7bf93-18ca-40d5-99e9-38b4eb69363e", "aws_cognito_pool_id": "ap-southeast-2_IYMvlZzmv", "aws_cognito_role_arn": "arn:aws:iam::YOUR_AWS_ACCOUNT:role/YOUR_Cognito_igvAuth_Role" -} +} \ No newline at end of file diff --git a/examples/oauth/oauth-config-google.json b/examples/oauth/oauth-config-google.json index b7974dc5f3..565db6e9e8 100644 --- a/examples/oauth/oauth-config-google.json +++ b/examples/oauth/oauth-config-google.json @@ -1,11 +1,7 @@ { - "client_id": "661332306814-gb4r480jnb6rres3iv8a5f48dms5ququu.apps.googleusercontent.com", - "client_secret": "MFqdc7apizmtb8n08Ze810V6", + "client_id": "YOUR_CLIENT_ID", + "client_secret": "YOUR_CLIENT_SECRET", "auth_uri": "https://accounts.google.com/o/oauth2/auth", "token_uri": "https://accounts.google.com/o/oauth2/token", - "auth_provider_x509_cert_url": "https://www.googleapis.com/oauth2/v1/certs", - "redirect_uris": [ - "http://localhost:60151/oauthCallback" - ], - "hosts": ["www.googleapis.com", "storage.cloud.google.com", "storage.googleapis.com"] + "auth_provider_x509_cert_url": "https://www.googleapis.com/oauth2/v1/certs" } \ No newline at end of file diff --git a/examples/oauth/oauth-config-microsoft.json b/examples/oauth/oauth-config-microsoft.json index 6b2189b259..0e2d5e121a 100644 --- a/examples/oauth/oauth-config-microsoft.json +++ b/examples/oauth/oauth-config-microsoft.json @@ -1,11 +1,11 @@ { - "client_id": "hbq82djj-qwer-7iub-j7zq-7i8nv72n48nq", - "client_secret": "cVAX64fXRikCLmtAow/cktrAtaHz/tmB3WHKhnbXN2Gq", - "authorization_endpoint": "https://login.microsoftonline.com/77nwe2q2-e53r-uq2p-7vdh-9z7px83zmtiv/oauth2/authorize", - "token_endpoint": "https://login.microsoftonline.com/77nwe2q2-e53r-wk32-7vdh-9z7px83zmtiv/oauth2/token", + "client_id": "YOUR_CLIENT_ID", + "client_secret": "YOUR_CLIENT_SECRET", + "authorization_endpoint": "https://login.microsoftonline.com/.../oauth2/authorize", + "token_endpoint": "https://login.microsoftonline.com/.../oauth2/token", "hosts": ["prod_host.mayo.edu", "dev_host.mayo.edu", "int_host.mayo.edu"], - "auth_provider": "Mayo Clinic", - "app_id_uri": "https://orgtools.onmicrosoft.com/6q9qk3mr-pu78-eu73-vx5a-nqw2aqidutm9", + "auth_provider": "YOUR PROVIDER", + "app_id_uri": "https://orgtools.onmicrosoft.com/...", "scope": "openid" } diff --git a/src/main/java/org/broad/igv/oauth/OAuthProvider.java b/src/main/java/org/broad/igv/oauth/OAuthProvider.java index be12784ed9..50b36d43f9 100644 --- a/src/main/java/org/broad/igv/oauth/OAuthProvider.java +++ b/src/main/java/org/broad/igv/oauth/OAuthProvider.java @@ -10,6 +10,7 @@ import org.broad.igv.batch.CommandListener; import org.broad.igv.event.IGVEventBus; import org.broad.igv.prefs.PreferencesManager; +import org.broad.igv.ui.IGV; import org.broad.igv.ui.util.MessageUtils; import org.broad.igv.util.AmazonUtils; import org.broad.igv.util.GoogleUtils; @@ -19,10 +20,7 @@ import java.awt.*; import java.io.IOException; -import java.net.URI; -import java.net.URISyntaxException; -import java.net.URL; -import java.net.URLDecoder; +import java.net.*; import java.time.Duration; import java.util.*; import java.util.prefs.Preferences; @@ -32,15 +30,12 @@ public class OAuthProvider { private static Logger log = LogManager.getLogger(OAuthProvider.class); private String authProvider = ""; - private String appIdURI = null; + private String appIdURI; public static String findString = null; public static String replaceString = null; - private static final String REFRESH_TOKEN_KEY = "oauth_refresh_token"; - private String state; // "RFC6749: An opaque value used by the client to maintain state" - private String portNumber = PreferencesManager.getPreferences().getPortNumber(); - private String redirectURI = "http%3A%2F%2Flocalhost%3A" + portNumber + "%2FoauthCallback"; + private String redirectURI; private String clientId; private String clientSecret; private String authEndpoint; @@ -54,13 +49,10 @@ public class OAuthProvider { private String currentUserID; private String currentUserEmail; private JsonObject response; - private JsonObject config; public OAuthProvider(JsonObject obj) throws IOException { - config = obj; - state = UUID.randomUUID().toString(); // "RFC6749: An opaque value used by the client to maintain state" // For backward compatibility @@ -84,6 +76,9 @@ public OAuthProvider(JsonObject obj) throws IOException { "authorization_endpoint/auth_uri or token_endpoint/token_uri"); } + String portNumber = PreferencesManager.getPreferences().getPortNumber(); + redirectURI = "http://localhost:" + portNumber + "/oauthCallback";; + // Optional or custom attributes, fail on runtime, depending on identity provider configuration clientSecret = obj.has("client_secret") ? obj.get("client_secret").getAsString() : null; setAuthProvider(obj.has("auth_provider") ? obj.get("auth_provider").getAsString() : authProvider); @@ -119,10 +114,6 @@ public OAuthProvider(JsonObject obj) throws IOException { String gsScope = "https://www.googleapis.com/auth/devstorage.read_only"; String emailScope = "https://www.googleapis.com/auth/userinfo.email"; scope = gsScope + "%20" + emailScope; - - } - if (authProvider == null) { - authProvider = "Google"; } } } @@ -131,14 +122,6 @@ public String getState() { return state; } - public String[] getHosts() { - return hosts; - } - - public void setHosts(String[] hosts) { - this.hosts = hosts; - } - /** * Send request to authorization provider to start the oauth 2.0 * authorization process. If the listener is up, wait for a callback @@ -168,7 +151,7 @@ public void openAuthorizationPage() throws IOException, URISyntaxException { } else { url = authEndpoint + "?state=" + state + - "&redirect_uri=" + redirectURI + + "&redirect_uri=" + URLEncoder.encode(redirectURI, "utf-8") + "&client_id=" + clientId + "&response_type=code"; @@ -185,7 +168,7 @@ public void openAuthorizationPage() throws IOException, URISyntaxException { if (desktop.isSupported(Desktop.Action.BROWSE)) { desktop.browse(new URI(url)); } else { - MessageUtils.showMessage("Copy this authorization URL into your web browser: " + url); + OAuthURLForm.open(IGV.getInstance().getMainFrame(), url); } } } @@ -201,7 +184,7 @@ public void setAuthorizationCode(String authorizationCode) throws IOException { if (clientSecret != null) { params.put("client_secret", clientSecret); } - params.put("redirect_uri", URLDecoder.decode(redirectURI, "utf-8")); + params.put("redirect_uri", redirectURI); params.put("grant_type", "authorization_code"); // set the resource if it necessary for the auth provider dwm08 @@ -446,7 +429,6 @@ public boolean appliesToUrl(URL url) { } } } - if (this.isGoogle()) { return GoogleUtils.isGoogleURL(url.toExternalForm()); } @@ -465,10 +447,6 @@ public void setResponse(JsonObject res) { response = res; } - public String getAuthProvider() { - return authProvider; - } - public void setAuthProvider(String authProvider) { this.authProvider = authProvider; } diff --git a/src/main/java/org/broad/igv/oauth/OAuthURLForm.java b/src/main/java/org/broad/igv/oauth/OAuthURLForm.java new file mode 100644 index 0000000000..494e85b99e --- /dev/null +++ b/src/main/java/org/broad/igv/oauth/OAuthURLForm.java @@ -0,0 +1,60 @@ +package org.broad.igv.oauth; + +import javax.swing.*; +import java.awt.*; +import java.awt.datatransfer.Clipboard; +import java.awt.datatransfer.StringSelection; +import java.awt.event.ActionEvent; +import java.awt.event.ActionListener; + +public class OAuthURLForm extends JDialog { + + private static JPanel getPanel(String url) { + JPanel panel = new JPanel(); + panel.setLayout(new BoxLayout(panel, BoxLayout.Y_AXIS)); + panel.setMinimumSize(new Dimension(400, 300)); + panel.setPreferredSize(new Dimension(400, 300)); + //this.add(panel); + + JPanel headerPanel = new JPanel(); + headerPanel.setLayout(new FlowLayout()); + JLabel label = new JLabel("Copy this authorization URL to your web browser"); + headerPanel.add(label); + + Button copyButton = new Button("Copy"); + copyButton.addActionListener(e -> { + StringSelection selection = new StringSelection(url); + Clipboard clipboard = Toolkit.getDefaultToolkit().getSystemClipboard(); + clipboard.setContents(selection, selection); + }); + headerPanel.add(copyButton); + panel.add(headerPanel); + + JTextArea textArea = new JTextArea(url); + textArea.setMargin(new Insets(10, 10, 10, 10)); + textArea.setLineWrap(true); + panel.add(textArea); + + panel.setAlignmentX(Component.LEFT_ALIGNMENT); + + return panel; + } + + public static void open(Frame owner, String url) { + + JPanel panel = getPanel(url); + JOptionPane.showMessageDialog(owner, + panel, + "OAuth Authorization URL", + JOptionPane.PLAIN_MESSAGE); + + } + + + public static void main(String[] args) { + + open(null, "https://docs.oracle.com/javase/tutorial/displayCode.html?code=https://docs.oracle.com/javase/tutorial/uiswing/examples/layout/BoxLayoutDemoProject/src/layout/BoxLayoutDemo.java"); + } +} + + diff --git a/src/main/java/org/broad/igv/oauth/OAuthUtils.java b/src/main/java/org/broad/igv/oauth/OAuthUtils.java index 12a1f03e5f..4ee4439cef 100644 --- a/src/main/java/org/broad/igv/oauth/OAuthUtils.java +++ b/src/main/java/org/broad/igv/oauth/OAuthUtils.java @@ -25,12 +25,13 @@ package org.broad.igv.oauth; -import com.google.gson.JsonArray; +import com.google.gson.JsonElement; import com.google.gson.JsonObject; import com.google.gson.JsonParser; import org.broad.igv.logging.*; import org.broad.igv.DirectoryManager; import org.broad.igv.prefs.PreferencesManager; +import org.broad.igv.ui.IGVMenuBar; import org.broad.igv.util.AmazonUtils; import org.broad.igv.util.GoogleUtils; import org.broad.igv.util.HttpUtils; @@ -39,11 +40,9 @@ import java.io.*; import java.net.URL; import java.net.URLConnection; -import java.util.HashMap; +import java.util.Iterator; import java.util.LinkedHashMap; import java.util.Map; -import java.util.regex.Matcher; -import java.util.regex.Pattern; import java.util.zip.GZIPInputStream; /** @@ -59,7 +58,7 @@ public class OAuthUtils { static OAuthProvider awsProvider; - static OAuthProvider defaultProvider; + static OAuthProvider googleProvider; static Map providerCache; @@ -89,8 +88,14 @@ public OAuthProvider getAWSProvider() { return awsProvider; } - public OAuthProvider getDefaultProvider() { - return defaultProvider; + public OAuthProvider getGooleProvider() throws IOException { + if (googleProvider == null) { + loadDefaultOauthProperties(); + if (IGVMenuBar.getInstance() != null) { + IGVMenuBar.getInstance().enableGoogleMenu(true); + } + } + return googleProvider; } private void fetchOauthProperties() throws IOException { @@ -117,14 +122,19 @@ private void fetchOauthProperties() throws IOException { log.error(e); } } + } - // Default (Google) provider - if (defaultProvider == null) { - String propString = loadAsString(PROPERTIES_URL); - defaultProvider = parseProviderJson(propString); - if (defaultProvider.getAuthProvider() == null || defaultProvider.getAuthProvider().isEmpty()) { - defaultProvider.setAuthProvider("Google"); - } + /** + * Load the default (Google) oAuth properties + * + * @throws IOException + */ + public void loadDefaultOauthProperties() throws IOException { + if (googleProvider == null) { + String json = loadAsString(PROPERTIES_URL); + JsonParser parser = new JsonParser(); + JsonObject obj = parser.parse(json).getAsJsonObject(); + googleProvider = parseProviderObject(obj); } } @@ -135,7 +145,7 @@ private void fetchOauthProperties() throws IOException { * @throws IOException */ public void updateOauthProvider(String provisioningURL) throws IOException { - if(provisioningURL != null && provisioningURL.trim().length() > 0) { + if (provisioningURL != null && provisioningURL.trim().length() > 0) { String json = loadAsString(provisioningURL); parseProviderJson(json); } @@ -143,7 +153,7 @@ public void updateOauthProvider(String provisioningURL) throws IOException { private String loadAsString(String urlOrPath) throws IOException { - if(HttpUtils.isRemoteURL(urlOrPath)) { + if (HttpUtils.isRemoteURL(urlOrPath)) { urlOrPath = HttpUtils.mapURL(urlOrPath); } InputStream is = null; @@ -163,54 +173,70 @@ private String loadAsString(String urlOrPath) throws IOException { /** * Parse Oauth provider configuration and update state *

        - * TODO -- refactor to remove side effects. * * @param json * @throws IOException */ - private OAuthProvider parseProviderJson(String json) throws IOException { + private void parseProviderJson(String json) throws IOException { JsonParser parser = new JsonParser(); - JsonObject obj = parser.parse(json).getAsJsonObject(); + JsonElement element = parser.parse(json); + if (element.isJsonArray()) { + Iterator iter = element.getAsJsonArray().iterator(); + while (iter.hasNext()) { + parseProviderObject(iter.next().getAsJsonObject()); + } + } else { + parseProviderObject(element.getAsJsonObject()); + } + + } + + private OAuthProvider parseProviderObject(JsonObject obj) throws IOException { OAuthProvider p = new OAuthProvider(obj); providerCache.put(p.getState(), p); - if (obj.has("auth_provider") && obj.get("auth_provider").getAsString().equals("Amazon")) { + if ((obj.has("auth_provider") + && obj.get("auth_provider").getAsString().equals("Amazon")) || + obj.has("aws_region")) { awsProvider = p; AmazonUtils.setCognitoConfig(obj); - } - if (p.isGoogle()) { - defaultProvider = p; + } else if (p.isGoogle()) { + googleProvider = p; + googleProvider.setAuthProvider("Google"); } return p; } + /** + * Called during authorization flow from CommandListener + * @param state + * @return + * @throws IOException + */ public OAuthProvider getProviderForState(String state) throws IOException { if (providerCache.containsKey(state)) { return providerCache.get(state); } else { // This should never happen, perhaps an error should be thrown. log.warn("No oAuth provider found for callback"); - return defaultProvider; + return null; } } - public OAuthProvider getProviderForURL(URL url) { + public OAuthProvider getProviderForURL(URL url) throws IOException { for (OAuthProvider provider : providerCache.values()) { if (provider.appliesToUrl(url)) { + if(provider.isGoogle()) { + IGVMenuBar.getInstance().enableGoogleMenu(true); + } return provider; } } - - // With a properly configured IGV we should never get here - if (GoogleUtils.isGoogleURL(url.toExternalForm()) && defaultProvider.isGoogle()) { - return defaultProvider; - } else { - return null; - } + return null; } /** * Open an input stream for reading a local or remote file. - + * * @param urlOrPath -- either an http URL or path to a local file. Can be gzipped * @return * @throws IOException diff --git a/src/main/java/org/broad/igv/prefs/IGVPreferences.java b/src/main/java/org/broad/igv/prefs/IGVPreferences.java index 82733cbf17..61bf5db497 100644 --- a/src/main/java/org/broad/igv/prefs/IGVPreferences.java +++ b/src/main/java/org/broad/igv/prefs/IGVPreferences.java @@ -44,6 +44,7 @@ import org.broad.igv.sam.mods.BaseModificationColors; import org.broad.igv.track.TrackType; import org.broad.igv.ui.IGV; +import org.broad.igv.ui.IGVMenuBar; import org.broad.igv.ui.UIConstants; import org.broad.igv.ui.color.ColorUtilities; import org.broad.igv.ui.color.PaletteColorTable; @@ -51,6 +52,7 @@ import java.awt.*; import java.io.File; +import java.io.IOException; import java.io.PrintWriter; import java.util.*; @@ -306,16 +308,25 @@ public void putAll(Map updatedPrefs) { checkForCommandListenerChanges(updatedPrefs); checkForAttributePanelChanges(updatedPrefs); checkForCircViewChanges(updatedPrefs); + checkForGoogleMenuChange(updatedPrefs); IGVEventBus.getInstance().post(new PreferencesChangeEvent()); + } + + private void checkForGoogleMenuChange(Map updatedPreferenceMap) { + if(updatedPreferenceMap.containsKey(ENABLE_GOOGLE_MENU) && IGV.hasInstance()) { + try { + IGVMenuBar.getInstance().enableGoogleMenu(getAsBoolean(ENABLE_GOOGLE_MENU)); + } catch (IOException e) { + log.error("Error enabling/disabling Google menu", e); + } + } } private void checkForAlignmentChanges(Map updatedPreferenceMap) { if (IGV.hasInstance()) { - final IGV igv = IGV.getInstance(); - boolean reloadSAM = false; for (String key : SAM_RELOAD_KEYS) { if (updatedPreferenceMap.containsKey(key)) { diff --git a/src/main/java/org/broad/igv/ui/IGV.java b/src/main/java/org/broad/igv/ui/IGV.java index 0517ef27b8..f9a9ac31f6 100644 --- a/src/main/java/org/broad/igv/ui/IGV.java +++ b/src/main/java/org/broad/igv/ui/IGV.java @@ -1161,10 +1161,6 @@ public void removeDataPanel(String name) { contentPane.getMainPanel().removeDataPanel(name); } - public void layoutMainPanel() { - contentPane.getMainPanel().doLayout(); - } - public MainPanel getMainPanel() { return contentPane.getMainPanel(); } @@ -1173,10 +1169,6 @@ public LinkedList getRecentSessionList() { return recentSessionList; } - public void setRecentSessionList(LinkedList recentSessionList) { - this.recentSessionList = recentSessionList; - } - public IGVContentPane getContentPane() { return contentPane; } diff --git a/src/main/java/org/broad/igv/ui/IGVMenuBar.java b/src/main/java/org/broad/igv/ui/IGVMenuBar.java index e380d833a0..1eaeef9c75 100644 --- a/src/main/java/org/broad/igv/ui/IGVMenuBar.java +++ b/src/main/java/org/broad/igv/ui/IGVMenuBar.java @@ -180,14 +180,19 @@ private List createMenus() { //extrasMenu.setVisible(false); menus.add(extrasMenu); - try { - googleMenu = createGoogleMenu(); - if (googleMenu != null) { - googleMenu.setVisible(PreferencesManager.getPreferences().getAsBoolean(ENABLE_GOOGLE_MENU)); - menus.add(googleMenu); + // Create a placehold Google menu. If not explicitly enabled it will remain invisible until triggered + // by loading a protected Google resource + googleMenu = new JMenu("Google"); + menus.add(googleMenu); + boolean enabled = PreferencesManager.getPreferences().getAsBoolean(ENABLE_GOOGLE_MENU); + googleMenu.setVisible(enabled); + + if (enabled) { + try { + enableGoogleMenu(enabled); + } catch (IOException e) { + log.error("Error creating google menu: " + e.getMessage()); } - } catch (IOException e) { - log.error("Error creating google menu: " + e.getMessage()); } try { @@ -1020,14 +1025,28 @@ public void menuCanceled(MenuEvent e) { return menu; } - private JMenu createGoogleMenu() throws IOException { + /** + * The Google menu is enabled dynamically to defer loading of oAuth properties until needed. + * * + * @return + * @throws IOException + */ + public void enableGoogleMenu(boolean enable) throws IOException { + + if(!enable) { + googleMenu.setVisible(false); + return; + } else if (googleMenu.isVisible()) { + // Already visible. + return; + } - // Dynamically name menu - dwm08 - final OAuthProvider oauth = OAuthUtils.getInstance().getDefaultProvider(); - if (oauth != null) { - JMenu menu = new JMenu("Google"); + googleMenu.setVisible(true); + final OAuthProvider oauth = OAuthUtils.getInstance().getGooleProvider(); + + if (oauth != null) { boolean isLoggedIn = oauth.isLoggedIn(); @@ -1042,7 +1061,7 @@ private JMenu createGoogleMenu() throws IOException { }); login.setEnabled(!isLoggedIn); - menu.add(login); + googleMenu.add(login); final JMenuItem logout = new JMenuItem("Logout "); @@ -1051,19 +1070,18 @@ private JMenu createGoogleMenu() throws IOException { GoogleUtils.setProjectID(null); }); logout.setEnabled(isLoggedIn); - menu.add(logout); + googleMenu.add(logout); final JMenuItem projectID = new JMenuItem("Enter Project ID ..."); projectID.addActionListener(e -> GoogleUtils.enterGoogleProjectID()); - menu.add(projectID); + googleMenu.add(projectID); - menu.addMenuListener(new MenuListener() { + googleMenu.addMenuListener(new MenuListener() { @Override public void menuSelected(MenuEvent e) { - OAuthProvider oAuthProvider = OAuthUtils.getInstance().getDefaultProvider(); - boolean loggedIn = oAuthProvider.isLoggedIn(); - if (loggedIn && oAuthProvider.getCurrentUserName() != null) { - login.setText(oAuthProvider.getCurrentUserName()); + boolean loggedIn = oauth.isLoggedIn(); + if (loggedIn && oauth.getCurrentUserName() != null) { + login.setText(oauth.getCurrentUserName()); } else { login.setText("Login ..."); } @@ -1082,9 +1100,6 @@ public void menuCanceled(MenuEvent e) { } }); - return menu; - } else { - return null; } } @@ -1156,11 +1171,6 @@ static void destroyInstance() { instance = null; } - public void enableGoogleMenu(boolean aBoolean) { - if (googleMenu != null) { - googleMenu.setVisible(aBoolean); - } - } @Override public void receiveEvent(final Object event) { diff --git a/src/main/java/org/broad/igv/ui/UIConstants.java b/src/main/java/org/broad/igv/ui/UIConstants.java index 24259a9622..fbefdddd00 100644 --- a/src/main/java/org/broad/igv/ui/UIConstants.java +++ b/src/main/java/org/broad/igv/ui/UIConstants.java @@ -91,7 +91,7 @@ public class UIConstants { final public static String OVERWRITE_SESSION_MESSAGE = "Opening a session will unload all current data. " + "
        Are you sure you wish to continue?"; final public static String CANNOT_ACCESS_SERVER_GENOME_LIST = "The Genome server is currently inaccessible."; - final public static int NUMBER_OF_RECENT_SESSIONS_TO_LIST = 3; + final public static int NUMBER_OF_RECENT_SESSIONS_TO_LIST = 10; final public static String DEFAULT_SESSION_FILE = "igv_session" + Globals.SESSION_FILE_EXTENSION; static final public String SERVER_BASE_URL = "http://www.broadinstitute.org/"; static final public Color LIGHT_YELLOW = new Color(255, 244, 201); diff --git a/src/main/java/org/broad/igv/ui/action/LoadFromURLMenuAction.java b/src/main/java/org/broad/igv/ui/action/LoadFromURLMenuAction.java index 6dac928b86..9c8473c975 100644 --- a/src/main/java/org/broad/igv/ui/action/LoadFromURLMenuAction.java +++ b/src/main/java/org/broad/igv/ui/action/LoadFromURLMenuAction.java @@ -142,24 +142,12 @@ private void checkURLs(String[] urls) { for (String url : urls) { if (url.startsWith("s3://")) { checkAWSAccessbility(url); - } else if (GoogleUtils.isGoogleURL(url)) { - enableGoogleMenu(); } else if (url.startsWith("ftp://")) { MessageUtils.showMessage("FTP protocol is not supported"); } } } - - private void enableGoogleMenu() { - - if (!PreferencesManager.getPreferences().getAsBoolean(Constants.ENABLE_GOOGLE_MENU)) { - PreferencesManager.getPreferences().put(Constants.ENABLE_GOOGLE_MENU, true); - IGVMenuBar.getInstance().enableGoogleMenu(true); - } - } - - private void checkAWSAccessbility(String url) { try { // If AWS support is active, check if objects are in accessible tiers via Load URL menu... diff --git a/src/main/java/org/broad/igv/util/HttpUtils.java b/src/main/java/org/broad/igv/util/HttpUtils.java index aebc9d8e65..78410909f1 100644 --- a/src/main/java/org/broad/igv/util/HttpUtils.java +++ b/src/main/java/org/broad/igv/util/HttpUtils.java @@ -57,7 +57,6 @@ import java.util.*; import java.util.regex.Matcher; import java.util.regex.Pattern; -import java.util.regex.PatternSyntaxException; import static org.broad.igv.prefs.Constants.*; import static org.broad.igv.util.stream.SeekableServiceStream.WEBSERVICE_URL; @@ -854,6 +853,9 @@ else if (code >= 400) { throw new FileNotFoundException(message); } else if (code == 401) { OAuthProvider provider = OAuthUtils.getInstance().getProviderForURL(url); + if(provider == null && GoogleUtils.isGoogleURL(url.toExternalForm())) { + provider = OAuthUtils.getInstance().getGooleProvider(); + } if (provider != null && retries == 0) { if (!provider.isLoggedIn()) { provider.checkLogin(); From 4e6ca00435cca85c407e492663bcce044240804f Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Wed, 13 Sep 2023 20:27:55 -0700 Subject: [PATCH 111/116] Fetch cognito credentials only as needed. Fixes #1311 (#1391) --- .../java/org/broad/igv/util/AmazonUtils.java | 111 ++++++++++-------- 1 file changed, 62 insertions(+), 49 deletions(-) diff --git a/src/main/java/org/broad/igv/util/AmazonUtils.java b/src/main/java/org/broad/igv/util/AmazonUtils.java index af6b6f08c7..7378596c02 100644 --- a/src/main/java/org/broad/igv/util/AmazonUtils.java +++ b/src/main/java/org/broad/igv/util/AmazonUtils.java @@ -36,6 +36,7 @@ import java.net.URL; import java.text.ParseException; import java.text.SimpleDateFormat; +import java.time.Instant; import java.util.*; import java.util.concurrent.CompletableFuture; import java.util.stream.Collectors; @@ -50,6 +51,8 @@ public class AmazonUtils { private static Region AWSREGION; private static Boolean awsCredentialsPresent = null; + private static Credentials cognitoAWSCredentials = null; + /** * Maps s3:// URLs to presigned URLs @@ -135,65 +138,67 @@ private static Region getAWSREGION() { */ public static Credentials GetCognitoAWSCredentials() { - OAuthProvider provider = OAuthUtils.getInstance().getAWSProvider(); + if (cognitoAWSCredentials == null || isExpired(cognitoAWSCredentials)) { + log.debug("fetch credentials"); + OAuthProvider provider = OAuthUtils.getInstance().getAWSProvider(); - JsonObject igv_oauth_conf = GetCognitoConfig(); - JsonObject response = provider.getResponse(); + JsonObject igv_oauth_conf = GetCognitoConfig(); + JsonObject response = provider.getResponse(); - // Handle non-user initiated S3 auth (IGV early startup), i.e user-specified GenomesLoader - if (response == null) { - // Go back to auth flow, not auth'd yet - checkLogin(); - response = provider.getResponse(); - } + // Handle non-user initiated S3 auth (IGV early startup), i.e user-specified GenomesLoader + if (response == null) { + // Go back to auth flow, not auth'd yet + checkLogin(); + response = provider.getResponse(); + } - JsonObject payload = JWTParser.getPayload(response.get("id_token").getAsString()); + JsonObject payload = JWTParser.getPayload(response.get("id_token").getAsString()); - log.debug("JWT payload id token: " + payload); + log.debug("JWT payload id token: " + payload); - // Collect necessary information from federated IdP for Authentication purposes - String idTokenStr = response.get("id_token").getAsString(); - String idProvider = payload.get("iss").toString().replace("https://", "") - .replace("\"", ""); - String email = payload.get("email").getAsString(); - String federatedPoolId = igv_oauth_conf.get("aws_cognito_fed_pool_id").getAsString(); - String cognitoRoleARN = igv_oauth_conf.get("aws_cognito_role_arn").getAsString(); + // Collect necessary information from federated IdP for Authentication purposes + String idTokenStr = response.get("id_token").getAsString(); + String idProvider = payload.get("iss").toString().replace("https://", "") + .replace("\"", ""); + String email = payload.get("email").getAsString(); + String federatedPoolId = igv_oauth_conf.get("aws_cognito_fed_pool_id").getAsString(); + String cognitoRoleARN = igv_oauth_conf.get("aws_cognito_role_arn").getAsString(); - HashMap logins = new HashMap<>(); - logins.put(idProvider, idTokenStr); + HashMap logins = new HashMap<>(); + logins.put(idProvider, idTokenStr); - // Avoid "software.amazon.awssdk.core.exception.SdkClientException: Unable to load credentials from any of the providers in the chain AwsCredentialsProviderChain(" - // The use of the AnonymousCredentialsProvider essentially bypasses the provider chain's requirement to access ~/.aws/credentials. - // https://stackoverflow.com/questions/36604024/sts-saml-and-java-sdk-unable-to-load-aws-credentials-from-any-provider-in-the-c - AnonymousCredentialsProvider anoCredProv = AnonymousCredentialsProvider.create(); + // Avoid "software.amazon.awssdk.core.exception.SdkClientException: Unable to load credentials from any of the providers in the chain AwsCredentialsProviderChain(" + // The use of the AnonymousCredentialsProvider essentially bypasses the provider chain's requirement to access ~/.aws/credentials. + // https://stackoverflow.com/questions/36604024/sts-saml-and-java-sdk-unable-to-load-aws-credentials-from-any-provider-in-the-c + AnonymousCredentialsProvider anoCredProv = AnonymousCredentialsProvider.create(); - // https://sdk.amazonaws.com/java/api/latest/software/amazon/awssdk/services/cognitoidentity/CognitoIdentityClient.html - // Build the Cognito client - CognitoIdentityClientBuilder cognitoIdentityBuilder = CognitoIdentityClient.builder(); + // https://sdk.amazonaws.com/java/api/latest/software/amazon/awssdk/services/cognitoidentity/CognitoIdentityClient.html + // Build the Cognito client + CognitoIdentityClientBuilder cognitoIdentityBuilder = CognitoIdentityClient.builder(); - cognitoIdentityBuilder.region(getAWSREGION()).credentialsProvider(anoCredProv); - cognitoIdentityClient = cognitoIdentityBuilder.build(); + cognitoIdentityBuilder.region(getAWSREGION()).credentialsProvider(anoCredProv); + cognitoIdentityClient = cognitoIdentityBuilder.build(); - // https://docs.aws.amazon.com/cognito/latest/developerguide/authentication-flow.html - // Basic (Classic) Authflow - // Uses AssumeRoleWithWebIdentity and facilitates CloudTrail org.broad.igv.logging. Uses one more request but provides user traceability. - GetIdRequest.Builder idrequest = GetIdRequest.builder().identityPoolId(federatedPoolId) - .logins(logins); - GetIdResponse idResult = cognitoIdentityClient.getId(idrequest.build()); + // https://docs.aws.amazon.com/cognito/latest/developerguide/authentication-flow.html + // Basic (Classic) Authflow + // Uses AssumeRoleWithWebIdentity and facilitates CloudTrail org.broad.igv.logging. Uses one more request but provides user traceability. + GetIdRequest.Builder idrequest = GetIdRequest.builder().identityPoolId(federatedPoolId) + .logins(logins); + GetIdResponse idResult = cognitoIdentityClient.getId(idrequest.build()); - GetOpenIdTokenRequest.Builder openidrequest = GetOpenIdTokenRequest.builder().logins(logins).identityId(idResult.identityId()); - GetOpenIdTokenResponse openId = cognitoIdentityClient.getOpenIdToken(openidrequest.build()); + GetOpenIdTokenRequest.Builder openidrequest = GetOpenIdTokenRequest.builder().logins(logins).identityId(idResult.identityId()); + GetOpenIdTokenResponse openId = cognitoIdentityClient.getOpenIdToken(openidrequest.build()); - AssumeRoleWithWebIdentityRequest.Builder webidrequest = AssumeRoleWithWebIdentityRequest.builder().webIdentityToken(openId.token()) - .roleSessionName(email) - .roleArn(cognitoRoleARN); + AssumeRoleWithWebIdentityRequest.Builder webidrequest = AssumeRoleWithWebIdentityRequest.builder().webIdentityToken(openId.token()) + .roleSessionName(email) + .roleArn(cognitoRoleARN); - AssumeRoleWithWebIdentityResponse stsClientResponse = StsClient.builder().credentialsProvider(anoCredProv) - .region(getAWSREGION()) - .build() - .assumeRoleWithWebIdentity(webidrequest.build()); + AssumeRoleWithWebIdentityResponse stsClientResponse = StsClient.builder().credentialsProvider(anoCredProv) + .region(getAWSREGION()) + .build() + .assumeRoleWithWebIdentity(webidrequest.build()); // // Enhanced (Simplified) Authflow // // Major drawback: Does not store federated user information on CloudTrail only authenticated role name appears in logs. @@ -211,7 +216,17 @@ public static Credentials GetCognitoAWSCredentials() { // // return authedRes.credentials() - return stsClientResponse.credentials(); + cognitoAWSCredentials = stsClientResponse.credentials(); + } + return cognitoAWSCredentials; + } + + /** + * @param cognitoAWSCredentials + * @return true if credentials are due to expire within next 10 seconds* + */ + private static boolean isExpired(Credentials cognitoAWSCredentials) { + return cognitoAWSCredentials.expiration().minusSeconds(10).isBefore(Instant.now()); } @@ -269,7 +284,7 @@ public static HeadObjectResponse getObjectMetadata(String bucket, String key) { .bucket(bucket) .key(key).build(); HeadObjectResponse HeadObjRes = s3Client.headObject(HeadObjReq); - log.debug("getObjectMetadata(): "+HeadObjRes.toString()); + log.debug("getObjectMetadata(): " + HeadObjRes.toString()); return HeadObjRes; } @@ -404,8 +419,6 @@ private static List getReadableBuckets(List buckets) { public static ArrayList ListBucketObjects(String bucketName, String prefix) { ArrayList objects = new ArrayList<>(); - log.debug("Listing objects for bucketName: " + bucketName); - if (GetCognitoConfig() != null) { OAuthUtils.getInstance().getAWSProvider().getAccessToken(); updateS3Client(GetCognitoAWSCredentials()); @@ -530,7 +543,7 @@ public static boolean isPresignedURL(String urlString) { public static String updatePresignedURL(String urlString) throws IOException { String s3UrlString = presignedToS3Map.get(urlString); - if(s3UrlString == null) { + if (s3UrlString == null) { throw new RuntimeException("Unrecognized presigned url: " + urlString); } else { return translateAmazonCloudURL(s3UrlString); From b5b9fd134d3bafe2fc438a74208b1e03de32fda6 Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Thu, 14 Sep 2023 13:39:29 -0700 Subject: [PATCH 112/116] oAuth (#1392) * Support PKCE (https://oauth.net/2/pkce/) * minor refactorings --- .../org/broad/igv/batch/CommandListener.java | 3 +- .../org/broad/igv/oauth/OAuthProvider.java | 209 +++++++++--------- .../java/org/broad/igv/oauth/OAuthUtils.java | 36 +-- .../java/org/broad/igv/oauth/PKCEUtils.java | 27 +++ .../java/org/broad/igv/ui/IGVMenuBar.java | 132 +++++------ .../igv/ui/util/ApplicationStatusBar.java | 28 ++- .../java/org/broad/igv/util/HttpUtils.java | 5 +- 7 files changed, 241 insertions(+), 199 deletions(-) create mode 100644 src/main/java/org/broad/igv/oauth/PKCEUtils.java diff --git a/src/main/java/org/broad/igv/batch/CommandListener.java b/src/main/java/org/broad/igv/batch/CommandListener.java index 5b3f6fffca..6cad61678a 100755 --- a/src/main/java/org/broad/igv/batch/CommandListener.java +++ b/src/main/java/org/broad/igv/batch/CommandListener.java @@ -37,7 +37,6 @@ import org.broad.igv.ui.IGV; import org.broad.igv.ui.util.MessageUtils; import org.broad.igv.ui.util.UIUtilities; -import org.broad.igv.util.HttpUtils; import org.broad.igv.util.StringUtils; import java.awt.*; @@ -228,7 +227,7 @@ private void processClientSession(CommandExecutor cmdExe) throws IOException { if (params.containsKey("error")) { sendTextResponse(out, "Error authorizing IGV: " + params.get("error")); } else if (params.containsKey("code")) { - provider.setAuthorizationCode(params.get("code")); + provider.fetchAccessToken(params.get("code")); sendTextResponse(out, "Authorization successful. You may close this tab."); } else if (params.containsKey("token")) { // Very doubtful this is ever called -- its not a normal OAuth flow diff --git a/src/main/java/org/broad/igv/oauth/OAuthProvider.java b/src/main/java/org/broad/igv/oauth/OAuthProvider.java index 50b36d43f9..92105ad1c0 100644 --- a/src/main/java/org/broad/igv/oauth/OAuthProvider.java +++ b/src/main/java/org/broad/igv/oauth/OAuthProvider.java @@ -21,9 +21,9 @@ import java.awt.*; import java.io.IOException; import java.net.*; +import java.security.NoSuchAlgorithmException; import java.time.Duration; import java.util.*; -import java.util.prefs.Preferences; public class OAuthProvider { @@ -33,7 +33,6 @@ public class OAuthProvider { private String appIdURI; public static String findString = null; public static String replaceString = null; - private static final String REFRESH_TOKEN_KEY = "oauth_refresh_token"; private String state; // "RFC6749: An opaque value used by the client to maintain state" private String redirectURI; private String clientId; @@ -42,6 +41,9 @@ public class OAuthProvider { private String tokenEndpoint; private String accessToken; private String refreshToken; + private String codeChallenge; + private String codeVerifier; + private String codeChallengeMethod; private long expirationTime; // in milliseconds private String scope; private String[] hosts; @@ -61,39 +63,39 @@ public OAuthProvider(JsonObject obj) throws IOException { } // Mandatory attributes, fail hard if not present - try { - clientId = obj.get("client_id").getAsString(); - authEndpoint = obj.has("auth_uri") ? - obj.get("auth_uri").getAsString() : - obj.get("authorization_endpoint").getAsString(); - tokenEndpoint = obj.has("token_uri") ? - obj.get("token_uri").getAsString() : - obj.get("token_endpoint").getAsString(); - - } catch (Exception e) { - log.error(e); - throw new IOException("oauthConfig is missing crucial attributes such as: client_id, " + - "authorization_endpoint/auth_uri or token_endpoint/token_uri"); + if (!(obj.has("client_id") && + (obj.has("auth_uri") || obj.has("authorization_endpoint")) && + (obj.has("token_uri") || obj.has("token_endpoint")) && + obj.has("client_secret"))) { + throw new RuntimeException("oauthConfig is missing crucial attributes such as: client_id, client_secret, " + + "authorization_endpoint/auth_uri, or token_endpoint/token_uri."); } - String portNumber = PreferencesManager.getPreferences().getPortNumber(); - redirectURI = "http://localhost:" + portNumber + "/oauthCallback";; - - // Optional or custom attributes, fail on runtime, depending on identity provider configuration + clientId = obj.get("client_id").getAsString(); + authEndpoint = obj.has("auth_uri") ? + obj.get("auth_uri").getAsString() : + obj.get("authorization_endpoint").getAsString(); + tokenEndpoint = obj.has("token_uri") ? + obj.get("token_uri").getAsString() : + obj.get("token_endpoint").getAsString(); clientSecret = obj.has("client_secret") ? obj.get("client_secret").getAsString() : null; - setAuthProvider(obj.has("auth_provider") ? obj.get("auth_provider").getAsString() : authProvider); - // app ID URI is a Microsoft - appIdURI = obj.has("app_id_uri") ? - obj.get("app_id_uri").getAsString() : - obj.has("resource") ? obj.get("resource").getAsString() : null; - - - findString = obj.has("find_string") ? obj.get("find_string").getAsString() : null; - replaceString = obj.has("replace_string") ? obj.get("replace_string").getAsString() : null; + // Optional attributes + if (obj.has("auth_provider")) { + authProvider = obj.get("auth_provider").getAsString(); + } if (obj.has("scope")) { scope = obj.get("scope").getAsString(); + } else if (isGoogle()) { + String gsScope = "https://www.googleapis.com/auth/devstorage.read_only"; + String emailScope = "https://www.googleapis.com/auth/userinfo.email"; + scope = gsScope + "%20" + emailScope; + } + appIdURI = obj.has("app_id_uri") ? // Microsoft Azure. + obj.get("app_id_uri").getAsString() : + obj.has("resource") ? obj.get("resource").getAsString() : null; + if (obj.has("hosts")) { // hosts element may be an array or a single string - put in hosts array either way JsonElement hostsElement = obj.get("hosts"); @@ -108,14 +110,12 @@ public OAuthProvider(JsonObject obj) throws IOException { } } - // Special Google properties - if (isGoogle()) { - if (scope == null) { - String gsScope = "https://www.googleapis.com/auth/devstorage.read_only"; - String emailScope = "https://www.googleapis.com/auth/userinfo.email"; - scope = gsScope + "%20" + emailScope; - } - } + String portNumber = PreferencesManager.getPreferences().getPortNumber(); + redirectURI = "http://localhost:" + portNumber + "/oauthCallback"; + + // Deprecated properties -- for backward compatibility + findString = obj.has("find_string") ? obj.get("find_string").getAsString() : null; + replaceString = obj.has("replace_string") ? obj.get("replace_string").getAsString() : null; } public String getState() { @@ -134,26 +134,38 @@ public String getState() { */ public void openAuthorizationPage() throws IOException, URISyntaxException { - String url; - // If the port listener is not on, try starting it if (!CommandListener.isListening()) { CommandListener.start(); } - // if the listener is not active, prompt the user - // for the access token + // if the listener is not active, prompt the user for the access token if (!CommandListener.isListening()) { - String ac = MessageUtils.showInputDialog("The IGV port listener is required for OAuth authentication. If you have an access token enter it here."); + String ac = MessageUtils.showInputDialog( + "The IGV port listener is off and is required for OAuth authentication through IGV
        . " + + "If you have an access token obtained by other means enter it here."); if (ac != null) { setAccessToken(ac); } } else { + // Generate PKCE challenge and verifier + codeVerifier = PKCEUtils.generateCodeVerifier(); + try { + codeChallenge = PKCEUtils.generateCodeChallange(codeVerifier); + codeChallengeMethod = "S256"; + } catch (NoSuchAlgorithmException e) { + codeChallenge = codeVerifier; + codeChallengeMethod = "plain"; + log.error("Error encoding PKCE challenge", e); + } - url = authEndpoint + "?state=" + state + + String url = authEndpoint + + "?state=" + state + "&redirect_uri=" + URLEncoder.encode(redirectURI, "utf-8") + "&client_id=" + clientId + - "&response_type=code"; + "&response_type=code" + + "&code_challenge=" + codeChallenge + + "&code_challenge_method=" + codeChallengeMethod; if (scope != null) { url += "&scope=" + scope; @@ -174,7 +186,7 @@ public void openAuthorizationPage() throws IOException, URISyntaxException { } // Called from port listener (org.broad.igv.batch.CommandListener) upon receiving the oauth request with a "code" parameter - public void setAuthorizationCode(String authorizationCode) throws IOException { + public void fetchAccessToken(String authorizationCode) throws IOException { URL url = HttpUtils.createURL(tokenEndpoint); @@ -184,10 +196,13 @@ public void setAuthorizationCode(String authorizationCode) throws IOException { if (clientSecret != null) { params.put("client_secret", clientSecret); } + params.put("state", state); params.put("redirect_uri", redirectURI); params.put("grant_type", "authorization_code"); + params.put("code_verifier", codeVerifier); + - // set the resource if it necessary for the auth provider dwm08 + // set the resource if necessary for the auth provider if (appIdURI != null) { params.put("resource", appIdURI); } @@ -197,11 +212,10 @@ public void setAuthorizationCode(String authorizationCode) throws IOException { String res = HttpUtils.getInstance().doPost(url, params); JsonParser parser = new JsonParser(); - setResponse(parser.parse(res).getAsJsonObject()); - + response = parser.parse(res).getAsJsonObject(); accessToken = response.get("access_token").getAsString(); refreshToken = response.get("refresh_token").getAsString(); - expirationTime = System.currentTimeMillis() + (response.get("expires_in").getAsInt() * 1000); + expirationTime = System.currentTimeMillis() + response.get("expires_in").getAsInt() * 1000; // Populate this class with user profile attributes if (response.has("id_token")) { @@ -241,13 +255,14 @@ public void setAccessToken(String accessToken) { */ private void refreshAccessToken() throws IOException { - // properties moved to early init dwm08 - //if (clientId == null) fetchOauthProperties(); + log.debug("Refresh access token"); Map params = new HashMap(); params.put("refresh_token", refreshToken); params.put("client_id", clientId); - params.put("client_secret", clientSecret); + if (clientSecret != null) { + params.put("client_secret", clientSecret); + } params.put("grant_type", "refresh_token"); // set the resource if it necessary for the auth provider dwm08 @@ -258,16 +273,17 @@ private void refreshAccessToken() throws IOException { // Poke the token refresh endpoint to get new access key URL url = HttpUtils.createURL(tokenEndpoint); - String response = HttpUtils.getInstance().doPost(url, params); + String responseString = HttpUtils.getInstance().doPost(url, params); JsonParser parser = new JsonParser(); + response = parser.parse(responseString).getAsJsonObject(); - setResponse(parser.parse(response).getAsJsonObject()); - JsonObject obj = getResponse(); - - JsonPrimitive atprim = obj.getAsJsonPrimitive("access_token"); + JsonPrimitive atprim = response.getAsJsonPrimitive("access_token"); if (atprim != null) { - accessToken = obj.getAsJsonPrimitive("access_token").getAsString(); - expirationTime = System.currentTimeMillis() + (obj.getAsJsonPrimitive("expires_in").getAsInt() * 1000); + accessToken = response.getAsJsonPrimitive("access_token").getAsString(); + if (response.has("refresh_token")) { + refreshToken = response.getAsJsonPrimitive("refresh_token").getAsString(); + } + expirationTime = System.currentTimeMillis() + response.getAsJsonPrimitive("expires_in").getAsInt() * 1000; } else { // Refresh token has failed, reauthorize from scratch reauthorize(); @@ -331,36 +347,6 @@ public Duration getExpirationTime() { return expiration; } - public class AuthStateEvent { - boolean authenticated; - String authProvider; - String userName; - String email; - - // Assuming that if this event is called, we are indeed autz/authn'd - public AuthStateEvent(boolean authenticated, String authProvider, String userName) { - this.authenticated = authenticated; - this.authProvider = authProvider; - this.userName = userName; - } - - public boolean isAuthenticated() { - return authenticated; - } - - public String getAuthProvider() { - return authProvider; - } - - public String getUserName() { - return userName; - } - - public String getEmail() { - return currentUserEmail; - } - } - public boolean isLoggedIn() { return accessToken != null; } @@ -369,22 +355,18 @@ public String getCurrentUserName() { return currentUserName != null ? currentUserName : (currentUserEmail != null ? currentUserEmail : currentUserID); } + public String getCurrentUserEmail() { + return currentUserEmail; + } public void logout() { accessToken = null; refreshToken = null; expirationTime = -1; currentUserName = null; - removeRefreshToken(); + IGVEventBus.getInstance().post(new AuthStateEvent(false, this.authProvider, null)); } - private void removeRefreshToken() { - try { - Preferences.userRoot().remove(REFRESH_TOKEN_KEY); - } catch (Exception e) { - log.error("Error removing oauth refresh token", e); - } - } /** * If not logged in, attempt to login @@ -443,12 +425,39 @@ public JsonObject getResponse() { return response; } - public void setResponse(JsonObject res) { - response = res; - } - public void setAuthProvider(String authProvider) { this.authProvider = authProvider; } + public String getAuthProvider() { + return authProvider; + } + + public static class AuthStateEvent { + boolean authenticated; + String authProvider; + String userName; + + // Assuming that if this event is called, we are indeed autz/authn'd + public AuthStateEvent(boolean authenticated, String authProvider, String userName) { + this.authenticated = authenticated; + this.authProvider = authProvider; + this.userName = userName; + } + + public boolean isAuthenticated() { + return authenticated; + } + + public String getAuthProvider() { + return authProvider; + } + + public String getUserName() { + return userName; + } + } } + + + diff --git a/src/main/java/org/broad/igv/oauth/OAuthUtils.java b/src/main/java/org/broad/igv/oauth/OAuthUtils.java index 4ee4439cef..3463a058b9 100644 --- a/src/main/java/org/broad/igv/oauth/OAuthUtils.java +++ b/src/main/java/org/broad/igv/oauth/OAuthUtils.java @@ -32,14 +32,15 @@ import org.broad.igv.DirectoryManager; import org.broad.igv.prefs.PreferencesManager; import org.broad.igv.ui.IGVMenuBar; +import org.broad.igv.ui.util.MessageUtils; import org.broad.igv.util.AmazonUtils; -import org.broad.igv.util.GoogleUtils; import org.broad.igv.util.HttpUtils; import java.io.*; import java.net.URL; import java.net.URLConnection; +import java.util.Collection; import java.util.Iterator; import java.util.LinkedHashMap; import java.util.Map; @@ -88,11 +89,17 @@ public OAuthProvider getAWSProvider() { return awsProvider; } - public OAuthProvider getGooleProvider() throws IOException { + public OAuthProvider getGoogleProvider() { if (googleProvider == null) { - loadDefaultOauthProperties(); - if (IGVMenuBar.getInstance() != null) { - IGVMenuBar.getInstance().enableGoogleMenu(true); + try { + log.info("Loading Google oAuth properties"); + googleProvider = loadDefaultOauthProperties(); + if (IGVMenuBar.getInstance() != null) { + IGVMenuBar.getInstance().enableGoogleMenu(true); + } + } catch (IOException e) { + log.error("Error loading Google oAuth properties", e); + MessageUtils.showErrorMessage("Error loading Google oAuth properties", e); } } return googleProvider; @@ -129,13 +136,11 @@ private void fetchOauthProperties() throws IOException { * * @throws IOException */ - public void loadDefaultOauthProperties() throws IOException { - if (googleProvider == null) { - String json = loadAsString(PROPERTIES_URL); - JsonParser parser = new JsonParser(); - JsonObject obj = parser.parse(json).getAsJsonObject(); - googleProvider = parseProviderObject(obj); - } + public OAuthProvider loadDefaultOauthProperties() throws IOException { + String json = loadAsString(PROPERTIES_URL); + JsonParser parser = new JsonParser(); + JsonObject obj = parser.parse(json).getAsJsonObject(); + return parseProviderObject(obj); } /** @@ -208,6 +213,7 @@ private OAuthProvider parseProviderObject(JsonObject obj) throws IOException { /** * Called during authorization flow from CommandListener + * * @param state * @return * @throws IOException @@ -225,7 +231,7 @@ public OAuthProvider getProviderForState(String state) throws IOException { public OAuthProvider getProviderForURL(URL url) throws IOException { for (OAuthProvider provider : providerCache.values()) { if (provider.appliesToUrl(url)) { - if(provider.isGoogle()) { + if (provider.isGoogle()) { IGVMenuBar.getInstance().enableGoogleMenu(true); } return provider; @@ -234,6 +240,10 @@ public OAuthProvider getProviderForURL(URL url) throws IOException { return null; } + public Collection getAllProviders() { + return providerCache.values(); + } + /** * Open an input stream for reading a local or remote file. * diff --git a/src/main/java/org/broad/igv/oauth/PKCEUtils.java b/src/main/java/org/broad/igv/oauth/PKCEUtils.java new file mode 100644 index 0000000000..8353ecf284 --- /dev/null +++ b/src/main/java/org/broad/igv/oauth/PKCEUtils.java @@ -0,0 +1,27 @@ +package org.broad.igv.oauth; + +import java.io.UnsupportedEncodingException; +import java.security.MessageDigest; +import java.security.NoSuchAlgorithmException; +import java.security.SecureRandom; +import java.util.Base64; + + +// Credit: https://www.appsdeveloperblog.com/pkce-code-verifier-and-code-challenge-in-java/ +public class PKCEUtils { + + public static String generateCodeVerifier() throws UnsupportedEncodingException { + SecureRandom secureRandom = new SecureRandom(); + byte[] codeVerifier = new byte[32]; + secureRandom.nextBytes(codeVerifier); + return Base64.getUrlEncoder().withoutPadding().encodeToString(codeVerifier); + } + + public static String generateCodeChallange(String codeVerifier) throws UnsupportedEncodingException, NoSuchAlgorithmException { + byte[] bytes = codeVerifier.getBytes("US-ASCII"); + MessageDigest messageDigest = MessageDigest.getInstance("SHA-256"); + messageDigest.update(bytes, 0, bytes.length); + byte[] digest = messageDigest.digest(); + return Base64.getUrlEncoder().withoutPadding().encodeToString(digest); + } +} \ No newline at end of file diff --git a/src/main/java/org/broad/igv/ui/IGVMenuBar.java b/src/main/java/org/broad/igv/ui/IGVMenuBar.java index 1eaeef9c75..1537a7fcd6 100644 --- a/src/main/java/org/broad/igv/ui/IGVMenuBar.java +++ b/src/main/java/org/broad/igv/ui/IGVMenuBar.java @@ -36,6 +36,7 @@ import org.broad.igv.event.IGVEventObserver; import org.broad.igv.feature.genome.GenomeManager; import org.broad.igv.feature.genome.GenomeUtils; +import org.broad.igv.prefs.IGVPreferences; import org.broad.igv.track.AttributeManager; import org.broad.igv.track.Track; import org.broad.igv.util.GoogleUtils; @@ -182,24 +183,19 @@ private List createMenus() { // Create a placehold Google menu. If not explicitly enabled it will remain invisible until triggered // by loading a protected Google resource - googleMenu = new JMenu("Google"); - menus.add(googleMenu); - boolean enabled = PreferencesManager.getPreferences().getAsBoolean(ENABLE_GOOGLE_MENU); - googleMenu.setVisible(enabled); - - if (enabled) { - try { - enableGoogleMenu(enabled); - } catch (IOException e) { - log.error("Error creating google menu: " + e.getMessage()); - } + try { + googleMenu = createGoogleMenu(); + boolean enabled = PreferencesManager.getPreferences().getAsBoolean(ENABLE_GOOGLE_MENU); + enableGoogleMenu(enabled); + menus.add(googleMenu); + } catch (IOException e) { + log.error("Error creating google menu: " + e.getMessage()); } try { AWSMenu = createAWSMenu(); AWSMenu.setVisible(AmazonUtils.isAwsProviderPresent()); menus.add(AWSMenu); - } catch (IOException e) { log.error("Error creating the Amazon AWS menu: " + e.getMessage()); AWSMenu.setVisible(false); @@ -1025,82 +1021,72 @@ public void menuCanceled(MenuEvent e) { return menu; } - /** - * The Google menu is enabled dynamically to defer loading of oAuth properties until needed. - * * - * @return - * @throws IOException - */ - public void enableGoogleMenu(boolean enable) throws IOException { - - if(!enable) { - googleMenu.setVisible(false); - return; - } else if (googleMenu.isVisible()) { - // Already visible. - return; - } + private JMenu createGoogleMenu() { + googleMenu = new JMenu("Google"); + final JMenuItem login = new JMenuItem("Login ... "); + login.addActionListener(e -> { + try { + OAuthUtils.getInstance().getGoogleProvider().openAuthorizationPage(); + } catch (Exception ex) { + MessageUtils.showErrorMessage("Error fetching oAuth tokens. See log for details", ex); + log.error("Error fetching oAuth tokens", ex); + } - googleMenu.setVisible(true); - final OAuthProvider oauth = OAuthUtils.getInstance().getGooleProvider(); + }); + googleMenu.add(login); - if (oauth != null) { + final JMenuItem logout = new JMenuItem("Logout "); + logout.addActionListener(e -> { + OAuthUtils.getInstance().getGoogleProvider().logout(); + GoogleUtils.setProjectID(null); + }); + googleMenu.add(logout); - boolean isLoggedIn = oauth.isLoggedIn(); + final JMenuItem projectID = new JMenuItem("Enter Project ID ..."); + projectID.addActionListener(e -> GoogleUtils.enterGoogleProjectID()); + googleMenu.add(projectID); - final JMenuItem login = new JMenuItem("Login ... "); - login.addActionListener(e -> { - try { - oauth.openAuthorizationPage(); - } catch (Exception ex) { - MessageUtils.showErrorMessage("Error fetching oAuth tokens. See log for details", ex); - log.error("Error fetching oAuth tokens", ex); + googleMenu.addMenuListener(new MenuListener() { + @Override + public void menuSelected(MenuEvent e) { + OAuthProvider oauth = OAuthUtils.getInstance().getGoogleProvider(); + boolean loggedIn = oauth.isLoggedIn(); + if (loggedIn && oauth.getCurrentUserName() != null) { + login.setText(oauth.getCurrentUserName()); + } else { + login.setText("Login ..."); } + login.setEnabled(!loggedIn); + logout.setEnabled(loggedIn); + } - }); - login.setEnabled(!isLoggedIn); - googleMenu.add(login); + @Override + public void menuDeselected(MenuEvent e) { + } - final JMenuItem logout = new JMenuItem("Logout "); - logout.addActionListener(e -> { - oauth.logout(); - GoogleUtils.setProjectID(null); - }); - logout.setEnabled(isLoggedIn); - googleMenu.add(logout); - - final JMenuItem projectID = new JMenuItem("Enter Project ID ..."); - projectID.addActionListener(e -> GoogleUtils.enterGoogleProjectID()); - googleMenu.add(projectID); - - googleMenu.addMenuListener(new MenuListener() { - @Override - public void menuSelected(MenuEvent e) { - boolean loggedIn = oauth.isLoggedIn(); - if (loggedIn && oauth.getCurrentUserName() != null) { - login.setText(oauth.getCurrentUserName()); - } else { - login.setText("Login ..."); - } - login.setEnabled(!loggedIn); - logout.setEnabled(loggedIn); - } + @Override + public void menuCanceled(MenuEvent e) { - @Override - public void menuDeselected(MenuEvent e) { + } - } + }); - @Override - public void menuCanceled(MenuEvent e) { + return googleMenu; + } - } - }); - } + /** + * The Google menu is enabled dynamically to defer loading of oAuth properties until needed. + * * + * + * @return + * @throws IOException + */ + public void enableGoogleMenu(boolean enable) throws IOException { + googleMenu.setVisible(enable); } // public void enableRemoveGenomes() { diff --git a/src/main/java/org/broad/igv/ui/util/ApplicationStatusBar.java b/src/main/java/org/broad/igv/ui/util/ApplicationStatusBar.java index 952411d4ed..4eb3b4f8f5 100644 --- a/src/main/java/org/broad/igv/ui/util/ApplicationStatusBar.java +++ b/src/main/java/org/broad/igv/ui/util/ApplicationStatusBar.java @@ -30,6 +30,7 @@ import org.broad.igv.event.StopEvent; import org.broad.igv.logging.*; import org.broad.igv.oauth.OAuthProvider; +import org.broad.igv.oauth.OAuthUtils; import org.broad.igv.ui.FontManager; import org.broad.igv.event.IGVEventBus; import org.broad.igv.event.IGVEventObserver; @@ -157,16 +158,23 @@ public void enableStopButton(boolean enable) { @Override public void receiveEvent(Object event) { - if(event instanceof OAuthProvider.AuthStateEvent) { - boolean isAuthed = ((OAuthProvider.AuthStateEvent) event).isAuthenticated(); - String authProvider = ((OAuthProvider.AuthStateEvent) event).getAuthProvider(); - String userName = ((OAuthProvider.AuthStateEvent) event).getUserName(); - String email = ((OAuthProvider.AuthStateEvent) event).getEmail(); - - if (isAuthed) { - setMessage3("Logged in as: " + email + " via " + authProvider); - } else { - setMessage3(""); + if (event instanceof OAuthProvider.AuthStateEvent) { + + String msg = ""; + for (OAuthProvider provider : OAuthUtils.getInstance().getAllProviders()) { + if (provider.isLoggedIn()) { + if(msg.length() == 0) { + msg += "Logged in as: "; + } else { + msg += "; "; + } + String user = provider.getCurrentUserEmail(); + if(user == null || user.length() == 0) { + user = provider.getCurrentUserName(); + } + msg += user + " via " + provider.getAuthProvider(); + } + setMessage3(msg); } } } diff --git a/src/main/java/org/broad/igv/util/HttpUtils.java b/src/main/java/org/broad/igv/util/HttpUtils.java index 78410909f1..349c9b3701 100644 --- a/src/main/java/org/broad/igv/util/HttpUtils.java +++ b/src/main/java/org/broad/igv/util/HttpUtils.java @@ -320,7 +320,10 @@ public String doPost(URL url, Map params) throws IOException { conn.getOutputStream().write(postDataBytes); StringBuilder response = new StringBuilder(); + System.out.println(conn.getResponseMessage()); Reader in = new BufferedReader(new InputStreamReader(conn.getInputStream(), "UTF-8")); + + for (int c; (c = in.read()) >= 0; ) { response.append((char) c); } @@ -854,7 +857,7 @@ else if (code >= 400) { } else if (code == 401) { OAuthProvider provider = OAuthUtils.getInstance().getProviderForURL(url); if(provider == null && GoogleUtils.isGoogleURL(url.toExternalForm())) { - provider = OAuthUtils.getInstance().getGooleProvider(); + provider = OAuthUtils.getInstance().getGoogleProvider(); } if (provider != null && retries == 0) { if (!provider.isLoggedIn()) { From 9fa24916316257aa178c8ad60e4aa5d64e721152 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Sun, 17 Sep 2023 23:26:56 -0700 Subject: [PATCH 113/116] oauth code cleanup --- examples/oauth/oauth-config-amazon.json | 16 +- src/main/java/org/broad/igv/Globals.java | 2 +- .../org/broad/igv/batch/CommandListener.java | 6 +- .../org/broad/igv/oauth/OAuthProvider.java | 40 ++-- .../org/broad/igv/oauth/OAuthURLForm.java | 5 - .../java/org/broad/igv/oauth/OAuthUtils.java | 59 ++--- .../org/broad/igv/oauth/OauthListener.java | 208 ++++++++++++++++++ .../java/org/broad/igv/util/AmazonUtils.java | 5 +- 8 files changed, 279 insertions(+), 62 deletions(-) create mode 100755 src/main/java/org/broad/igv/oauth/OauthListener.java diff --git a/examples/oauth/oauth-config-amazon.json b/examples/oauth/oauth-config-amazon.json index 410cd9022c..74ee4d2327 100644 --- a/examples/oauth/oauth-config-amazon.json +++ b/examples/oauth/oauth-config-amazon.json @@ -3,13 +3,13 @@ "project_id": "igv", "auth_provider": "Amazon", "auth_provider_x509_cert_url": "https://www.googleapis.com/oauth2/v1/certs", - "aws_region": "ap-southeast-2", + "aws_region": "us-east-1", "scope": "email%20openid%20profile", - "client_id": "3f4ujenfmr77tg12iofbebpkoh", - "client_secret": "en1q6638m4dogrr6erhosetim67sjilc6htjnfmf6ljk2q3j9og", - "authorization_endpoint": "https://igv-demo.auth.ap-southeast-2.amazoncognito.com/login", - "token_endpoint": "https://igv-demo.auth.ap-southeast-2.amazoncognito.com/token", - "aws_cognito_fed_pool_id": "ap-southeast-2:15b7bf93-18ca-40d5-99e9-38b4eb69363e", - "aws_cognito_pool_id": "ap-southeast-2_IYMvlZzmv", - "aws_cognito_role_arn": "arn:aws:iam::YOUR_AWS_ACCOUNT:role/YOUR_Cognito_igvAuth_Role" + "client_id": "eu7vd09a3dsfasdfd7r4kjomqg", + "client_secret": "s2416djkq5liigbrjdhktdsdgerefktsv04f9p810a3nc9", + "authorization_endpoint": "https://igv-demo.auth.us-east-1.amazoncognito.com/login", + "token_endpoint": "https://igv-demo.auth.us-east-1.amazoncognito.com/token", + "aws_cognito_fed_pool_id": "us-east-1:2926325e-0938-4dfc-840a-fa8a3e4f63f7", + "aws_cognito_pool_id": "us-east-1_v98erojsdkfj", + "aws_cognito_role_arn": "arn:aws:iam::3940820579:role/Cognito_igvAuth_Role" } \ No newline at end of file diff --git a/src/main/java/org/broad/igv/Globals.java b/src/main/java/org/broad/igv/Globals.java index 502eebaf88..0cf1b5a089 100644 --- a/src/main/java/org/broad/igv/Globals.java +++ b/src/main/java/org/broad/igv/Globals.java @@ -55,7 +55,7 @@ public class Globals { private static boolean testing = false; public static int CONNECT_TIMEOUT = 20000; // 20 seconds public static int READ_TIMEOUT = 1000 * 3 * 60; // 3 minutes - public static int TOKEN_EXPIRE_GRACE_TIME = 1000 * 1 * 60; // 1 minute + public static int TOKEN_EXPIRE_GRACE_TIME = 1000 * 60; // 1 minute /** * Field description diff --git a/src/main/java/org/broad/igv/batch/CommandListener.java b/src/main/java/org/broad/igv/batch/CommandListener.java index 6cad61678a..95a6ca76de 100755 --- a/src/main/java/org/broad/igv/batch/CommandListener.java +++ b/src/main/java/org/broad/igv/batch/CommandListener.java @@ -222,7 +222,6 @@ private void processClientSession(CommandExecutor cmdExe) throws IOException { // Detect oauth callback if (command.equals("/oauthCallback")) { - OAuthProvider provider = OAuthUtils.getInstance().getProviderForState(params.get("state")); if (params.containsKey("error")) { sendTextResponse(out, "Error authorizing IGV: " + params.get("error")); @@ -237,12 +236,11 @@ private void processClientSession(CommandExecutor cmdExe) throws IOException { } else { sendTextResponse(out, "Unsuccessful authorization response: " + inputLine); } - - if (PreferencesManager.getPreferences().getAsBoolean(Constants.PORT_ENABLED) == false) { // Turn off port halt(); } + } else { // Process the request. result = processGet(command, params, cmdExe); // Send no response if result is "OK". @@ -312,7 +310,7 @@ private void closeSockets() { private static final String NO_CACHE = "Cache-Control: no-cache, no-store"; private static final String ACCESS_CONTROL_ALLOW_ORIGIN = "Access-Control-Allow-Origin: *"; private static final String ACCESS_CONTROL_ALLOW_HEADERS = "Access-Control-Allow-Headers: access-control-allow-origin"; - + private void sendTextResponse(PrintWriter out, String result) { sendHTTPResponse(out, result, "text/html", "GET"); } diff --git a/src/main/java/org/broad/igv/oauth/OAuthProvider.java b/src/main/java/org/broad/igv/oauth/OAuthProvider.java index 92105ad1c0..f352bd6781 100644 --- a/src/main/java/org/broad/igv/oauth/OAuthProvider.java +++ b/src/main/java/org/broad/igv/oauth/OAuthProvider.java @@ -29,6 +29,8 @@ public class OAuthProvider { private static Logger log = LogManager.getLogger(OAuthProvider.class); + private static int TOKEN_EXPIRE_GRACE_TIME = 1000 * 60; // 1 minute + private String authProvider = ""; private String appIdURI; public static String findString = null; @@ -65,8 +67,7 @@ public OAuthProvider(JsonObject obj) throws IOException { // Mandatory attributes, fail hard if not present if (!(obj.has("client_id") && (obj.has("auth_uri") || obj.has("authorization_endpoint")) && - (obj.has("token_uri") || obj.has("token_endpoint")) && - obj.has("client_secret"))) { + (obj.has("token_uri") || obj.has("token_endpoint")))) { throw new RuntimeException("oauthConfig is missing crucial attributes such as: client_id, client_secret, " + "authorization_endpoint/auth_uri, or token_endpoint/token_uri."); } @@ -110,8 +111,26 @@ public OAuthProvider(JsonObject obj) throws IOException { } } - String portNumber = PreferencesManager.getPreferences().getPortNumber(); - redirectURI = "http://localhost:" + portNumber + "/oauthCallback"; + if (obj.has("redirect_uris")) { + JsonArray urisArray = obj.get("redirect_uris").getAsJsonArray(); + redirectURI = urisArray.get(0).getAsString(); + } else if (obj.has("redirect_uri")) { + redirectURI = obj.get("redirect_uri").getAsString(); + } else { + String portNumber = PreferencesManager.getPreferences().getPortNumber(); + redirectURI = "http://localhost:" + portNumber + "/oauthCallback"; + } + + // Generate PKCE challenge and verifier + codeVerifier = PKCEUtils.generateCodeVerifier(); + try { + codeChallenge = PKCEUtils.generateCodeChallange(codeVerifier); + codeChallengeMethod = "S256"; + } catch (Exception e) { + codeChallenge = codeVerifier; + codeChallengeMethod = "plain"; + log.error("Error encoding PKCE challenge", e); + } // Deprecated properties -- for backward compatibility findString = obj.has("find_string") ? obj.get("find_string").getAsString() : null; @@ -148,16 +167,6 @@ public void openAuthorizationPage() throws IOException, URISyntaxException { setAccessToken(ac); } } else { - // Generate PKCE challenge and verifier - codeVerifier = PKCEUtils.generateCodeVerifier(); - try { - codeChallenge = PKCEUtils.generateCodeChallange(codeVerifier); - codeChallengeMethod = "S256"; - } catch (NoSuchAlgorithmException e) { - codeChallenge = codeVerifier; - codeChallengeMethod = "plain"; - log.error("Error encoding PKCE challenge", e); - } String url = authEndpoint + "?state=" + state + @@ -201,7 +210,6 @@ public void fetchAccessToken(String authorizationCode) throws IOException { params.put("grant_type", "authorization_code"); params.put("code_verifier", codeVerifier); - // set the resource if necessary for the auth provider if (appIdURI != null) { params.put("resource", appIdURI); @@ -324,7 +332,7 @@ public JsonObject fetchUserProfile(JsonObject jwt_payload) { public String getAccessToken() { // Check expiration time, with 1 minute cushion - if (accessToken == null || (System.currentTimeMillis() > (expirationTime - Globals.TOKEN_EXPIRE_GRACE_TIME))) { + if (accessToken == null || (System.currentTimeMillis() > (expirationTime - TOKEN_EXPIRE_GRACE_TIME))) { log.debug("Refreshing access token!"); if (refreshToken != null) { try { diff --git a/src/main/java/org/broad/igv/oauth/OAuthURLForm.java b/src/main/java/org/broad/igv/oauth/OAuthURLForm.java index 494e85b99e..4b8e9d8f6e 100644 --- a/src/main/java/org/broad/igv/oauth/OAuthURLForm.java +++ b/src/main/java/org/broad/igv/oauth/OAuthURLForm.java @@ -50,11 +50,6 @@ public static void open(Frame owner, String url) { } - - public static void main(String[] args) { - - open(null, "https://docs.oracle.com/javase/tutorial/displayCode.html?code=https://docs.oracle.com/javase/tutorial/uiswing/examples/layout/BoxLayoutDemoProject/src/layout/BoxLayoutDemo.java"); - } } diff --git a/src/main/java/org/broad/igv/oauth/OAuthUtils.java b/src/main/java/org/broad/igv/oauth/OAuthUtils.java index 3463a058b9..8e67c11f20 100644 --- a/src/main/java/org/broad/igv/oauth/OAuthUtils.java +++ b/src/main/java/org/broad/igv/oauth/OAuthUtils.java @@ -73,39 +73,17 @@ public static synchronized OAuthUtils getInstance() { private OAuthUtils() { try { providerCache = new LinkedHashMap<>(); // Ordered (linked) map is important - fetchOauthProperties(); + fetchOauthConfigs(); } catch (Exception e) { log.error("Error fetching oAuth properties", e); } } /** - * Called by AWS code only + * Fetch user-configured oAuth configurations, if any* + * @throws IOException */ - public OAuthProvider getAWSProvider() { - if (awsProvider == null) { - throw new RuntimeException("AWS Oauth is not configured"); - } - return awsProvider; - } - - public OAuthProvider getGoogleProvider() { - if (googleProvider == null) { - try { - log.info("Loading Google oAuth properties"); - googleProvider = loadDefaultOauthProperties(); - if (IGVMenuBar.getInstance() != null) { - IGVMenuBar.getInstance().enableGoogleMenu(true); - } - } catch (IOException e) { - log.error("Error loading Google oAuth properties", e); - MessageUtils.showErrorMessage("Error loading Google oAuth properties", e); - } - } - return googleProvider; - } - - private void fetchOauthProperties() throws IOException { + private void fetchOauthConfigs() throws IOException { // Load a provider config specified in preferences String provisioningURL = PreferencesManager.getPreferences().getProvisioningURL(); @@ -131,12 +109,39 @@ private void fetchOauthProperties() throws IOException { } } + /** + * Called by AWS code only + */ + public OAuthProvider getAWSProvider() { + if (awsProvider == null) { + throw new RuntimeException("AWS Oauth is not configured"); + } + return awsProvider; + } + + public OAuthProvider getGoogleProvider() { + if (googleProvider == null) { + try { + log.info("Loading Google oAuth properties"); + googleProvider = loadDefaultOauthProperties(); + if (IGVMenuBar.getInstance() != null) { + IGVMenuBar.getInstance().enableGoogleMenu(true); + } + } catch (IOException e) { + log.error("Error loading Google oAuth properties", e); + MessageUtils.showErrorMessage("Error loading Google oAuth properties", e); + } + } + return googleProvider; + } + + /** * Load the default (Google) oAuth properties * * @throws IOException */ - public OAuthProvider loadDefaultOauthProperties() throws IOException { + private OAuthProvider loadDefaultOauthProperties() throws IOException { String json = loadAsString(PROPERTIES_URL); JsonParser parser = new JsonParser(); JsonObject obj = parser.parse(json).getAsJsonObject(); diff --git a/src/main/java/org/broad/igv/oauth/OauthListener.java b/src/main/java/org/broad/igv/oauth/OauthListener.java new file mode 100755 index 0000000000..52d05993ae --- /dev/null +++ b/src/main/java/org/broad/igv/oauth/OauthListener.java @@ -0,0 +1,208 @@ +/* + * The MIT License (MIT) + * + * Copyright (c) 2007-2015 Broad Institute + * + * Permission is hereby granted, free of charge, to any person obtaining a copy + * of this software and associated documentation files (the "Software"), to deal + * in the Software without restriction, including without limitation the rights + * to use, copy, modify, merge, publish, distribute, sublicense, and/or sell + * copies of the Software, and to permit persons to whom the Software is + * furnished to do so, subject to the following conditions: + * + * The above copyright notice and this permission notice shall be included in + * all copies or substantial portions of the Software. + * + * + * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR + * IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, + * FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE + * AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER + * LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, + * OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN + * THE SOFTWARE. + */ + +package org.broad.igv.oauth; + +import biz.source_code.base64Coder.Base64Coder; +import org.broad.igv.Globals; +import org.broad.igv.batch.CommandExecutor; +import org.broad.igv.feature.genome.GenomeManager; +import org.broad.igv.logging.LogManager; +import org.broad.igv.logging.Logger; +import org.broad.igv.prefs.Constants; +import org.broad.igv.prefs.PreferencesManager; +import org.broad.igv.ui.IGV; +import org.broad.igv.ui.util.MessageUtils; +import org.broad.igv.ui.util.UIUtilities; +import org.broad.igv.util.StringUtils; + +import java.awt.*; +import java.io.*; +import java.net.ServerSocket; +import java.net.Socket; +import java.net.URLDecoder; +import java.nio.channels.ClosedByInterruptException; +import java.security.NoSuchAlgorithmException; +import java.util.*; + +/** + * NOT CURRENTLY USED -- developed for dynamic redirectURIs (dynamic random port numers), possible with Google but not Amazaon + */ + +public class OauthListener implements Runnable { + + private static Logger log = LogManager.getLogger(OauthListener.class); + + private static final String CRLF = "\r\n"; + + private int port; + private OAuthProvider provider; + private Thread listenerThread; + + + public static synchronized void start(int port, OAuthProvider provider) { + OauthListener listener = null; + try { + listener = new OauthListener(port, provider); + listener.listenerThread.start(); + } catch (Exception e) { + log.error(e); + } + } + + private OauthListener(int port, OAuthProvider provider) { + this.port = port; + this.provider = provider; + listenerThread = new Thread(this); + } + + public void run() { + + try (ServerSocket serverSocket = new ServerSocket(port); + Socket clientSocket = serverSocket.accept();) { + + PrintWriter out = new PrintWriter(clientSocket.getOutputStream(), true); + BufferedReader in = new BufferedReader(new InputStreamReader(clientSocket.getInputStream())); + + String inputLine = in.readLine(); + System.out.println(inputLine); + + // Consume the remainder of the request, if any (typically request headers). This is important to free the connection. + String nextLine = in.readLine(); + while (nextLine != null && nextLine.length() > 0) { + nextLine = in.readLine(); + System.out.println(nextLine); + } + + String[] tokens = inputLine.split(" "); + if (tokens.length < 2) { + sendTextResponse(out, "ERROR unexpected oauth request: " + inputLine); + } else { + + String[] parts = tokens[1].split("\\?"); + Map params = parts.length < 2 ? new HashMap() : parseParameters(parts[1]); + + if (params.containsKey("error")) { + sendTextResponse(out, "Error authorizing IGV: " + params.get("error")); + } else if (params.containsKey("code")) { + provider.fetchAccessToken(params.get("code")); + sendTextResponse(out, "Authorization successful. You may close this tab."); + } else { + sendTextResponse(out, "Unsuccessful authorization response: " + inputLine); + } + } + } catch (java.net.BindException e) { + MessageUtils.showErrorMessage("Error opening listener for oAuth authorization", e); + } catch (Exception e) { + MessageUtils.showErrorMessage("Error opening listener for oAuth authorization", e); + log.error(e); + } + } + + + private static final String HTTP_RESPONSE = "HTTP/1.1 200 OK"; + private static final String HTTP_NO_RESPONSE = "HTTP/1.1 204 No Response"; + private static final String CONNECTION_CLOSE = "Connection: close"; + private static final String NO_CACHE = "Cache-Control: no-cache, no-store"; + private static final String ACCESS_CONTROL_ALLOW_ORIGIN = "Access-Control-Allow-Origin: *"; + + private void sendTextResponse(PrintWriter out, String result) { + sendHTTPResponse(out, result, "text/html", "GET"); + } + + private void sendHTTPResponse(PrintWriter out, String result, String contentType, String method) { + + out.print(result == null ? HTTP_NO_RESPONSE : HTTP_RESPONSE); + out.print(CRLF); + out.print(ACCESS_CONTROL_ALLOW_ORIGIN); + out.print(CRLF); + if (result != null) { + out.print("Content-Type: " + contentType); + out.print(CRLF); + out.print("Content-Length: " + (result.length())); + out.print(CRLF); + out.print(NO_CACHE); + out.print(CRLF); + out.print(CONNECTION_CLOSE); + out.print(CRLF); + + if (!method.equals("HEAD")) { + out.print(CRLF); + out.print(result); + out.print(CRLF); + } + } else { + out.print(CRLF); + } + out.close(); + } + + + /** + * Parse the html parameter string into a set of key-value pairs. Parameter values are + * url decoded with the exception of the "locus" parameter. + * + * @param parameterString + * @return + */ + private Map parseParameters(String parameterString) { + + // Do a partial decoding now (ampersands only) + parameterString = parameterString.replace("&", "&"); + + HashMap params = new HashMap(); + String[] kvPairs = parameterString.split("&"); + for (String kvString : kvPairs) { + // Split on the first "=", all others are part of the parameter value + String[] kv = kvString.split("=", 2); + if (kv.length == 1) { + params.put(kv[0], null); + } else { + String key = StringUtils.decodeURL(kv[0]); + String value = StringUtils.decodeURL(kv[1]); + params.put(key, value); + } + } + return params; + } + + /** + * Find and available port* + * @return + */ + public static int findFreePort() { + for (int port = 49152; port < 65536; port++) { + try (ServerSocket serverSocket = new ServerSocket(port)) { + if (serverSocket != null && serverSocket.getLocalPort() == port) { + return port; + } + } catch (IOException e) { + //Port is not available, try again + } + } + return -1; + } + +} diff --git a/src/main/java/org/broad/igv/util/AmazonUtils.java b/src/main/java/org/broad/igv/util/AmazonUtils.java index 7378596c02..2df905a817 100644 --- a/src/main/java/org/broad/igv/util/AmazonUtils.java +++ b/src/main/java/org/broad/igv/util/AmazonUtils.java @@ -53,6 +53,9 @@ public class AmazonUtils { private static Credentials cognitoAWSCredentials = null; + private static int TOKEN_EXPIRE_GRACE_TIME = 1000 * 60; // 1 minute + + /** * Maps s3:// URLs to presigned URLs @@ -576,7 +579,7 @@ private static boolean isPresignedURLValid(URL url) { try { long presignedTime = signedURLValidity(url); - isValidSignedUrl = presignedTime - System.currentTimeMillis() - Globals.TOKEN_EXPIRE_GRACE_TIME > 0; // Duration in milliseconds + isValidSignedUrl = presignedTime - System.currentTimeMillis() - TOKEN_EXPIRE_GRACE_TIME > 0; // Duration in milliseconds } catch (ParseException e) { log.error("The AWS signed URL date parameter X-Amz-Date has incorrect formatting"); isValidSignedUrl = false; From b948ec7c8b994b4017b0493afdcf627a51e6f0a5 Mon Sep 17 00:00:00 2001 From: Jim Robinson <933148+jrobinso@users.noreply.github.com> Date: Mon, 25 Sep 2023 16:33:31 -0700 Subject: [PATCH 114/116] Support "bedmethyl" format. See issue #1396 --- .../org/broad/igv/feature/FeatureType.java | 2 +- .../org/broad/igv/feature/FeatureUtils.java | 20 ++-- .../igv/feature/tribble/CodecFactory.java | 3 + .../igv/feature/tribble/IGVBEDCodec.java | 107 +++++++++++------- .../org/broad/igv/track/FeatureTrack.java | 10 +- .../org/broad/igv/util/ResourceLocator.java | 2 +- 6 files changed, 85 insertions(+), 59 deletions(-) diff --git a/src/main/java/org/broad/igv/feature/FeatureType.java b/src/main/java/org/broad/igv/feature/FeatureType.java index be33b08eb3..ade18e3bc0 100644 --- a/src/main/java/org/broad/igv/feature/FeatureType.java +++ b/src/main/java/org/broad/igv/feature/FeatureType.java @@ -30,7 +30,7 @@ * @author jrobinso */ public enum FeatureType { - OTHER, GENE, PROMOTER, MISC_RNA, REPEAT_REGION, LTR, MUTATION, BED, GAPPED_PEAK, SPLICE_JUNCTION + OTHER, GENE, PROMOTER, MISC_RNA, REPEAT_REGION, LTR, MUTATION, BED, GAPPED_PEAK, SPLICE_JUNCTION, BED_METHYL } diff --git a/src/main/java/org/broad/igv/feature/FeatureUtils.java b/src/main/java/org/broad/igv/feature/FeatureUtils.java index 7e755272b9..2964bf396b 100644 --- a/src/main/java/org/broad/igv/feature/FeatureUtils.java +++ b/src/main/java/org/broad/igv/feature/FeatureUtils.java @@ -159,7 +159,7 @@ public static Feature getFeatureCenteredAfter(double position, List features.size()-1) { + if (idx < 0 || idx > features.size() - 1) { return null; } else { return features.get(idx); @@ -214,10 +214,10 @@ public static Feature getFeatureClosest(double position, List private static int getDistance(final int position, final Feature f) { final int end = f.getEnd(); final int start = f.getStart(); - if(position >= start && position < end){ //within the feature + if (position >= start && position < end) { //within the feature return 0; - } else if(position >= end){ - return position - end +1; //after the feature + } else if (position >= end) { + return position - end + 1; //after the feature } else { return start - position; //before the feature } @@ -265,7 +265,7 @@ public static int getIndexBefore(double position, List featur * @param features * @return */ - private static int getIndexCenterAfter(double position, List features) { + private static int getIndexCenterAfter(double position, List features) { Feature first = features.get(0); Feature last = features.get(features.size() - 1); @@ -387,19 +387,23 @@ public static List getAllFeaturesAt(double position, for (int idx = startIdx; idx < features.size(); idx++) { Feature feature = features.get(idx); double start = feature.getStart() - (minWidth / 2); - if (start > position) { break; } - double end = feature.getEnd() + (minWidth / 2); - if (position >= start && position <= end) { if (returnList == null) returnList = new ArrayList(); returnList.add(feature); } } + // Sort features by distance from position (features closest to position listed first) + returnList.sort((o1, o2) -> { + double dist1 = Math.abs((o1.getStart() + (o1.getEnd() - o1.getStart()) / 2) - position); + double dist2 = Math.abs((o2.getStart() + (o2.getEnd() - o2.getStart()) / 2) - position); + return dist1 == dist2 ? 0 : dist1 > dist2 ? 1 : -1; + }); + return returnList; } diff --git a/src/main/java/org/broad/igv/feature/tribble/CodecFactory.java b/src/main/java/org/broad/igv/feature/tribble/CodecFactory.java index 55f7d43b62..9fec4c82a0 100644 --- a/src/main/java/org/broad/igv/feature/tribble/CodecFactory.java +++ b/src/main/java/org/broad/igv/feature/tribble/CodecFactory.java @@ -138,6 +138,9 @@ public static FeatureCodec getCodec(ResourceLocator locator, Genome genome) { return new UCSCGeneTableCodec(UCSCGeneTableCodec.Type.UCSCGENE, genome); case "genepredext": return new UCSCGeneTableCodec(UCSCGeneTableCodec.Type.GENEPRED_EXT, genome); + case "bedmethyl": + return new IGVBEDCodec(genome, FeatureType.BED_METHYL); + default: if (MUTCodec.isMutationAnnotationFile(locator)) { return new MUTCodec(path, genome); diff --git a/src/main/java/org/broad/igv/feature/tribble/IGVBEDCodec.java b/src/main/java/org/broad/igv/feature/tribble/IGVBEDCodec.java index 160bc1dc27..25d9ef6608 100644 --- a/src/main/java/org/broad/igv/feature/tribble/IGVBEDCodec.java +++ b/src/main/java/org/broad/igv/feature/tribble/IGVBEDCodec.java @@ -30,6 +30,7 @@ import org.broad.igv.feature.*; import org.broad.igv.feature.genome.Genome; import org.broad.igv.ui.color.ColorUtilities; +import org.broad.igv.ui.util.MessageUtils; import org.broad.igv.util.StringUtils; import htsjdk.tribble.Feature; @@ -45,7 +46,7 @@ * Date: Dec 20, 2009 * Time: 10:15:49 PM */ -public class IGVBEDCodec extends UCSCCodec { +public class IGVBEDCodec extends UCSCCodec { private static final Logger log = LogManager.getLogger(IGVBEDCodec.class); @@ -56,6 +57,8 @@ public class IGVBEDCodec extends UCSCCodec { private Genome genome; private Pattern delimiter = null; + private int maxColumnCount = Integer.MAX_VALUE; + public IGVBEDCodec() { this(null); } @@ -75,7 +78,7 @@ public BasicFeature decode(String[] tokens) { // The first 3 columns are non optional for BED. We will relax this // and only require 2. - int tokenCount = tokens.length; + int tokenCount = Math.min(tokens.length, maxColumnCount); if (tokenCount < 2) { return null; @@ -180,7 +183,6 @@ public BasicFeature decode(String[] tokens) { } } - // Color if (tokenCount > 8 && featureType != FeatureType.GAPPED_PEAK) { String colorString = tokens[8]; @@ -189,47 +191,70 @@ public BasicFeature decode(String[] tokens) { } } - // Exons - if (tokenCount > 11) { - createExons(start, tokens, feature, chr, feature.getStrand()); - //todo: some refactoring that allows this hack to be removed - if (featureType == FeatureType.SPLICE_JUNCTION) { - SpliceJunctionFeature junctionFeature = (SpliceJunctionFeature) feature; + if (featureType == FeatureType.BED_METHYL && tokenCount > 9) { + // Description from https://www.encodeproject.org/data-standards/wgbs/ + // 10. Coverage, or number of reads + // 11. Percentage of reads that show methylation at this position in the genome + Map attributes = new LinkedHashMap<>(); + if (tokenCount > 9) { + attributes.put("Coverage", tokens[9]); + } + if (tokenCount > 10) { + attributes.put("% of methylated reads", tokens[10]); + } + feature.setAttributes(attributes); + } else { + // Exons + try { + if (tokenCount > 11) { + createExons(start, tokens, feature, chr, feature.getStrand()); + //todo: some refactoring that allows this hack to be removed + if (featureType == FeatureType.SPLICE_JUNCTION) { + SpliceJunctionFeature junctionFeature = (SpliceJunctionFeature) feature; - List exons = feature.getExons(); + List exons = feature.getExons(); - junctionFeature.setJunctionStart(start + exons.get(0).getLength()); - junctionFeature.setJunctionEnd(end - exons.get(1).getLength()); + junctionFeature.setJunctionStart(start + exons.get(0).getLength()); + junctionFeature.setJunctionEnd(end - exons.get(1).getLength()); + } + } + } catch (NumberFormatException e) { + final String message = "Unexpected data in columns 10-12, expected exon count, exon starts, and exon sizes. Found: " + + tokens[9] + " " + tokens[10] + " " + tokens[11] + "
        Columns > 9 will be ignored"; + this.maxColumnCount = 9; + MessageUtils.showMessage(message); + log.warn(message, e); + return feature; } - } - if (tokenCount > 14 && featureType == FeatureType.GAPPED_PEAK) { - Map attributes = new LinkedHashMap<>(); - attributes.put("Signal Value", tokens[12]); - attributes.put("pValue (-log10)", tokens[13]); - attributes.put("qValue (-log10)", tokens[14]); - feature.setAttributes(attributes); - } else if (tokenCount > 13 && featureType == FeatureType.SPLICE_JUNCTION) { - try { - String[] startFlanking = tokens[12].split(","); - int[] startFlankingDeptyArray = new int[startFlanking.length]; - for (int i = 0; i < startFlanking.length; i++) { - startFlankingDeptyArray[i] = Integer.parseInt(startFlanking[i]); - } - String[] endFlanking = tokens[13].split(","); - int[] endFlankingDeptyArray = new int[endFlanking.length]; - for (int i = 0; i < endFlanking.length; i++) { - endFlankingDeptyArray[i] = Integer.parseInt(endFlanking[i]); + if (tokenCount > 14 && featureType == FeatureType.GAPPED_PEAK) { + Map attributes = new LinkedHashMap<>(); + attributes.put("Signal Value", tokens[12]); + attributes.put("pValue (-log10)", tokens[13]); + attributes.put("qValue (-log10)", tokens[14]); + feature.setAttributes(attributes); + } else if (tokenCount > 13 && featureType == FeatureType.SPLICE_JUNCTION) { + try { + String[] startFlanking = tokens[12].split(","); + int[] startFlankingDeptyArray = new int[startFlanking.length]; + for (int i = 0; i < startFlanking.length; i++) { + startFlankingDeptyArray[i] = Integer.parseInt(startFlanking[i]); + } + String[] endFlanking = tokens[13].split(","); + int[] endFlankingDeptyArray = new int[endFlanking.length]; + for (int i = 0; i < endFlanking.length; i++) { + endFlankingDeptyArray[i] = Integer.parseInt(endFlanking[i]); + } + ((SpliceJunctionFeature) feature).setStartFlankingRegionDepthArray(startFlankingDeptyArray); + ((SpliceJunctionFeature) feature).setEndFlankingRegionDepthArray(endFlankingDeptyArray); + } catch (NumberFormatException e) { + log.error("Error parsing flanking array", e); } - ((SpliceJunctionFeature) feature).setStartFlankingRegionDepthArray(startFlankingDeptyArray); - ((SpliceJunctionFeature) feature).setEndFlankingRegionDepthArray(endFlankingDeptyArray); - } catch (NumberFormatException e) { - log.error("Error parsing flanking array", e); } } - if(isCoding(feature)) { + if (isCoding(feature)) { FeatureUtils.computeReadingFrames(feature); } @@ -239,9 +264,9 @@ public BasicFeature decode(String[] tokens) { /** * Approximate test for a coding feature (transcript). BED format does not specify this explicitly. * Rules applied are - * + *

        * (1) feature has exons - * (2) feature has possible UTRs at both ends + * (2) feature has possible UTRs at both ends * (3) feature has strand * * @param feature @@ -267,8 +292,8 @@ public BasicFeature decode(String nextLine) { } // Bed files can be tab or whitespace delimited - if(delimiter == null) { - delimiter = trimLine.contains("\t") ? Globals.multiTabPattern : Globals.whitespacePattern; + if (delimiter == null) { + delimiter = trimLine.contains("\t") ? Globals.multiTabPattern : Globals.whitespacePattern; } tokens = delimiter.split(trimLine); @@ -298,7 +323,7 @@ public boolean canDecode(String path) { public static void createExons(int start, String[] tokens, BasicFeature gene, String chr, - Strand strand) throws NumberFormatException { + Strand strand) throws NumberFormatException { int cdStart = Integer.parseInt(tokens[6]); int cdEnd = Integer.parseInt(tokens[7]); @@ -329,8 +354,6 @@ public static void createExons(int start, String[] tokens, BasicFeature gene, St } - - /** * Encode a feature as a BED string. * diff --git a/src/main/java/org/broad/igv/track/FeatureTrack.java b/src/main/java/org/broad/igv/track/FeatureTrack.java index 09024285ac..9e05998523 100644 --- a/src/main/java/org/broad/igv/track/FeatureTrack.java +++ b/src/main/java/org/broad/igv/track/FeatureTrack.java @@ -378,7 +378,6 @@ public Renderer getRenderer() { */ public String getValueStringAt(String chr, double position, int mouseX, int mouseY, ReferenceFrame frame) { - if (showFeatures) { List allFeatures = getAllFeatureAt(position, mouseY, frame); @@ -388,7 +387,7 @@ public String getValueStringAt(String chr, double position, int mouseX, int mous StringBuffer buf = new StringBuffer(); boolean firstFeature = true; - int maxNumber = 100; + int maxNumber = IGV.getInstance().isShowDetailsOnClick() ? 100 : 10; int n = 1; for (Feature feature : allFeatures) { if (feature != null && feature instanceof IGVFeature) { @@ -406,17 +405,14 @@ public String getValueStringAt(String chr, double position, int mouseX, int mous buf.append("
        " + url + ""); } } - firstFeature = false; - if (n > maxNumber) { - buf.append("..."); + buf.append("


        >+ " + (allFeatures.size() - maxNumber) + " more ..."); break; } } n++; } - return buf.toString(); } else { int zoom = Math.max(0, frame.getZoom()); @@ -562,7 +558,7 @@ public List getFeaturesAtPositionInFeatureRow(double position, int feat if (possFeatures != null) { // give a minum 2 pixel or 1/2 bp window, otherwise very narrow features will be missed. double bpPerPixel = frame.getScale(); - double minWidth = Math.max(0.5, MINIMUM_FEATURE_SPACING * bpPerPixel); + double minWidth = Math.max(2, 3 * bpPerPixel); int maxFeatureLength = packedFeatures.getMaxFeatureLength(); featureList = FeatureUtils.getAllFeaturesAt(position, maxFeatureLength, minWidth, possFeatures); } diff --git a/src/main/java/org/broad/igv/util/ResourceLocator.java b/src/main/java/org/broad/igv/util/ResourceLocator.java index 1d15e59fab..3544116812 100644 --- a/src/main/java/org/broad/igv/util/ResourceLocator.java +++ b/src/main/java/org/broad/igv/util/ResourceLocator.java @@ -649,5 +649,5 @@ public String toString() { } static Set knownFormats = new HashSet<>(Arrays.asList("gff", "bed", "gtf", "gff3", - "seg", "bb", "bigbed", "bigwig", "bam", "cram", "vcf")); + "seg", "bb", "bigbed", "bigwig", "bam", "cram", "vcf", "bedmethyl")); } From 396c7e2eb0c1bcf07de87b40b5554f66c5699aff Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Tue, 26 Sep 2023 15:03:59 -0700 Subject: [PATCH 115/116] Bedmethyl tweak: handle mixed-delimiter files. See #1396 --- .../igv/feature/genome/GenomeListItem.java | 2 +- .../igv/feature/tribble/IGVBEDCodec.java | 46 ++++++++++--------- 2 files changed, 25 insertions(+), 23 deletions(-) diff --git a/src/main/java/org/broad/igv/feature/genome/GenomeListItem.java b/src/main/java/org/broad/igv/feature/genome/GenomeListItem.java index 83d2c26ace..fd3c3d8a3d 100644 --- a/src/main/java/org/broad/igv/feature/genome/GenomeListItem.java +++ b/src/main/java/org/broad/igv/feature/genome/GenomeListItem.java @@ -40,7 +40,7 @@ public class GenomeListItem { public static final GenomeListItem DOWNLOAD_ITEM; static { - DOWNLOAD_ITEM = new GenomeListItem("More...", "", "More..."); + DOWNLOAD_ITEM = new GenomeListItem("Click for more ...", "", "More..."); } diff --git a/src/main/java/org/broad/igv/feature/tribble/IGVBEDCodec.java b/src/main/java/org/broad/igv/feature/tribble/IGVBEDCodec.java index 25d9ef6608..1b70774d12 100644 --- a/src/main/java/org/broad/igv/feature/tribble/IGVBEDCodec.java +++ b/src/main/java/org/broad/igv/feature/tribble/IGVBEDCodec.java @@ -191,17 +191,18 @@ public BasicFeature decode(String[] tokens) { } } - if (featureType == FeatureType.BED_METHYL && tokenCount > 9) { + if (featureType == FeatureType.BED_METHYL && tokenCount > 10) { // Description from https://www.encodeproject.org/data-standards/wgbs/ // 10. Coverage, or number of reads // 11. Percentage of reads that show methylation at this position in the genome Map attributes = new LinkedHashMap<>(); - if (tokenCount > 9) { attributes.put("Coverage", tokens[9]); - } - if (tokenCount > 10) { - attributes.put("% of methylated reads", tokens[10]); - } + + // Columns after 10 are sometimes space delimited + int idx = tokens[10].indexOf(' '); + String value = idx > 0 ? tokens[10].substring(0, idx) : tokens[10]; + attributes.put("Percentage of reads that show methylation", value); + feature.setAttributes(attributes); } else { // Exons @@ -332,22 +333,23 @@ public static void createExons(int start, String[] tokens, BasicFeature gene, St String[] exonSizes = Globals.commaPattern.split(tokens[10]); String[] startsBuffer = Globals.commaPattern.split(tokens[11]); - int exonNumber = (strand == Strand.NEGATIVE ? exonCount : 1); - - if (startsBuffer.length == exonSizes.length) { - for (int i = 0; i < startsBuffer.length; i++) { - int exonStart = start + Integer.parseInt(startsBuffer[i]); - int exonEnd = exonStart + Integer.parseInt(exonSizes[i]); - Exon exon = new Exon(chr, exonStart, exonEnd, strand); - exon.setCodingStart(cdStart); - exon.setCodingEnd(cdEnd); - gene.addExon(exon); - - exon.setNumber(exonNumber); - if (strand == Strand.NEGATIVE) { - exonNumber--; - } else { - exonNumber++; + if(exonCount == exonSizes.length && exonCount == startsBuffer.length) { + int exonNumber = (strand == Strand.NEGATIVE ? exonCount : 1); + if (startsBuffer.length == exonSizes.length) { + for (int i = 0; i < startsBuffer.length; i++) { + int exonStart = start + Integer.parseInt(startsBuffer[i]); + int exonEnd = exonStart + Integer.parseInt(exonSizes[i]); + Exon exon = new Exon(chr, exonStart, exonEnd, strand); + exon.setCodingStart(cdStart); + exon.setCodingEnd(cdEnd); + gene.addExon(exon); + + exon.setNumber(exonNumber); + if (strand == Strand.NEGATIVE) { + exonNumber--; + } else { + exonNumber++; + } } } } From 227302e4be83ba9b57d88fd3834162328a5b4522 Mon Sep 17 00:00:00 2001 From: Louis Bergelson Date: Fri, 29 Sep 2023 19:43:11 -0400 Subject: [PATCH 116/116] Update to use Java 17 (#1315) * Update to use Java 17 * change to gradle 8.0.2 * minor changes to support java 17 * remove test module configuration and use gradle test platform instead * update configuration reference from defalt -> runtimeClasspath, also change some 11's to 17s * remove broken proguard stuff --- .github/workflows/gradle_test.yml | 13 +- build.gradle | 100 ++----- gradle/wrapper/gradle-wrapper.jar | Bin 55616 -> 61574 bytes gradle/wrapper/gradle-wrapper.properties | 3 +- gradlew | 276 +++++++++++------- gradlew.bat | 40 +-- scripts/test_suite.args | 75 ----- .../com/sanityinc/jargs/CmdLineParser.java | 6 +- .../org/broad/igv/feature/genome/Genome.java | 4 +- .../org/broad/igv/prefs/IGVPreferences.java | 21 +- .../broad/igv/sam/BaseAlignmentCounts.java | 16 +- .../broad/igv/sam/SparseAlignmentCounts.java | 7 +- .../org/broad/igv/sam/reader/BAMReader.java | 2 +- .../broad/igv/session/IGVSessionReader.java | 8 +- .../org/broad/igv/ui/action/MenuAction.java | 2 +- .../java/org/broad/igv/util/Utilities.java | 8 +- 16 files changed, 254 insertions(+), 327 deletions(-) delete mode 100644 scripts/test_suite.args diff --git a/.github/workflows/gradle_test.yml b/.github/workflows/gradle_test.yml index 75e06a740d..faae2a500d 100644 --- a/.github/workflows/gradle_test.yml +++ b/.github/workflows/gradle_test.yml @@ -7,13 +7,14 @@ jobs: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v2 - - name: Set up JDK 11 - uses: actions/setup-java@v1 + - uses: actions/checkout@v3 + - name: Set up JDK 17 + uses: actions/setup-java@v3 with: - java-version: 11 + java-version: 17 + distribution: temurin - name: Cache Gradle packages - uses: actions/cache@v2 + uses: actions/cache@v3 with: path: | ~/.gradle/caches @@ -30,4 +31,4 @@ jobs: # Restoring these files from a GitHub Actions cache might cause problems for future builds. run: | rm -f ~/.gradle/caches/modules-2/modules-2.lock - rm -f ~/.gradle/caches/modules-2/gc.properties \ No newline at end of file + rm -f ~/.gradle/caches/modules-2/gc.properties diff --git a/build.gradle b/build.gradle index 0f2a391c25..d1fdd2a04d 100644 --- a/build.gradle +++ b/build.gradle @@ -27,26 +27,25 @@ apply plugin: 'application' import org.apache.tools.ant.filters.ReplaceTokens -//import proguard.gradle.ProGuardTask - mainClassName = 'org.broad.igv.ui.Main' ext.moduleName = 'org.igv' -// Build sourceset for ProGuard buildscript { repositories { mavenCentral() } - dependencies { - [group: 'net.sf.proguard', name: 'proguard-gradle', version: '6.1.1'] - classpath 'net.sf.proguard:proguard-gradle:6.1.1' - } } repositories { mavenCentral() } +java { + toolchain { + languageVersion = JavaLanguageVersion.of(17) + } +} + sourceSets { main { resources { @@ -128,10 +127,13 @@ dependencies { ) testImplementation( - [group: 'junit', name: 'junit', version: '4.12'], + [group: 'junit', name: 'junit', version: '4.13'], [group: 'com.sparkjava', name: 'spark-core', version: '2.2'], [group: 'org.glassfish.jersey.core', name: 'jersey-common', version: '2.22.4'] ) + testRuntimeOnly( + [group: 'org.junit.vintage', name:'junit-vintage-engine', version:'5.8.2'] + ) } @@ -179,35 +181,7 @@ tasks.withType(Test) { systemProperties['org.xerial.snappy.tempdir'] = 'build/tmp' maxHeapSize = '2g' maxParallelForks = Runtime.runtime.availableProcessors().intdiv(2) ?: 1 -} - -compileTestJava { - inputs.property("moduleName", moduleName) - doFirst { - options.compilerArgs = [ - '--module-path', classpath.asPath, - '--add-modules', 'junit', - '--patch-module', "$moduleName=" + files(sourceSets.test.java.srcDirs).asPath, - ] - classpath = files() - } -} - -test { - inputs.property("moduleName", moduleName) - - doFirst { - jvmArgs = [ - '-Dcom.sun.xml.bind.v2.bytecode.ClassTailor.noOptimize', - '--module-path', classpath.asPath, - '--add-modules', 'junit', - '--add-modules', 'ALL-MODULE-PATH', - '--add-reads', "$moduleName=junit", - '@scripts/test_suite.args', - '--patch-module', "$moduleName=" + files(sourceSets.test.java.outputDir, sourceSets.main.output.resourcesDir).asPath, - ] - classpath = files() - } + useJUnitPlatform() } task createDist(type: Copy, dependsOn: jar) { @@ -229,10 +203,11 @@ task createDist(type: Copy, dependsOn: jar) { } // Copies all Maven-fetched dependency jars with copySpec { - from configurations.default { + from configurations.runtimeClasspath { exclude '**/*log4j*.jar' } into "lib" + duplicatesStrategy DuplicatesStrategy.EXCLUDE } with copySpec { from("genomes/sizes") {} into "lib/genomes" } @@ -244,32 +219,32 @@ tasks.distTar.enabled = false tasks.startScripts.enabled = false task createDistZip(type: Zip, dependsOn: createDist) { - archiveName = "IGV_${version}.zip" + archiveFileName = "IGV_${archiveVersion}.zip" from("${buildDir}/IGV-dist") - into "IGV_${version}" + into "IGV_${archiveVersion}" } task createLinuxDistZip(type: Zip, dependsOn: createDist) { - archiveName = "IGV_Linux_${version}.zip" + archiveFileName = "IGV_Linux_${archiveVersion}.zip" from("${buildDir}/IGV-dist") { exclude "*.bat" exclude "*.command" exclude 'igvtools*' exclude 'lib/genomes' } - into "IGV_Linux_${version}" + into "IGV_Linux_${archiveVersion}" } task createLinuxWithJavaDistZip(type: Zip, dependsOn: createDist) { - archiveName = "IGV_Linux_${version}_WithJava.zip" - with copySpec { from jdkBundleLinux into "jdk-11" } + archiveFileName = "IGV_Linux_${archiveVersion}_WithJava.zip" + with copySpec { from jdkBundleLinux into "jdk-17" } from("${buildDir}/IGV-dist") { exclude "*.bat" exclude "*.command" exclude 'igvtools*' exclude 'lib/genomes' } - into "IGV_Linux_${version}" + into "IGV_Linux_${archiveVersion}" doLast { if (jdkBundleLinux == "") { throw new GradleException("Required property not set: jdkBundleLinux"); @@ -278,26 +253,27 @@ task createLinuxWithJavaDistZip(type: Zip, dependsOn: createDist) { } task createMacDistZip(type: Zip, dependsOn: createDist) { - archiveName = "IGV_Mac_${version}.zip" + archiveFileName = "IGV_Mac_${archiveVersion}.zip" from("${buildDir}/IGV-dist") { exclude "*.bat" exclude "*_hidpi*" exclude "igvtools*" exclude 'lib/genomes' } - into "IGV_Mac_${version}" + + into "IGV_Mac_${archiveVersion}" } task createMacWithJavaDistZip(type: Zip, dependsOn: createDist) { - archiveName = "IGV_Mac_${version}_WithJava.zip" - with copySpec { from jdkBundleMac into "jdk-11" } + archiveFileName = "IGV_Mac_${archiveVersion}_WithJava.zip" + with copySpec { from jdkBundleMac into "jdk-17" } from("${buildDir}/IGV-dist") { exclude "*.bat" exclude "*_hidpi*" exclude "igvtools*" exclude 'lib/genomes' } - into "IGV_Mac_${version}" + into "IGV_Mac_${archiveVersion}" doLast { if (jdkBundleMac == "") { throw new GradleException("Required property not set: jdkBundleMac"); @@ -339,7 +315,7 @@ task createMacAppDist(type: Copy, dependsOn: createDist) { } task createMacAppDistZip(type: Zip, dependsOn: createMacAppDist) { - archiveName = "IGV_MacApp_${version}.zip" + archiveFileName = "IGV_MacApp_${archiveVersion}.zip" from("${buildDir}/IGV-MacApp-dist") doLast { @@ -348,8 +324,8 @@ task createMacAppDistZip(type: Zip, dependsOn: createMacAppDist) { } task createMacAppWithJavaDistZip(type: Zip, dependsOn: createMacAppDist) { - archiveName = "IGV_MacApp_${version}_WithJava.zip" - with copySpec { from jdkBundleMac into "IGV_${version}.app/Contents/jdk-11" } + archiveFileName = "IGV_MacApp_${archiveVersion}_WithJava.zip" + with copySpec { from jdkBundleMac into "IGV_${archiveVersion}.app/Contents/jdk-17" } from("${buildDir}/IGV-MacApp-dist") doLast { @@ -383,7 +359,7 @@ task createWinDist(type: Copy, dependsOn: createDist) { } task createWinWithJavaDist(type: Copy, dependsOn: createWinDist) { - with copySpec { from jdkBundleWindows into "IGV_${version}/jdk-11" } + with copySpec { from jdkBundleWindows into "IGV_${version}/jdk-17" } with copySpec { from("${buildDir}/IGV-WinExe-dist/IGV_${version}") { exclude 'installer.nsi' } into "IGV_${version}" @@ -465,7 +441,8 @@ task fullJar(type: Jar, dependsOn: jar) { "Application-Name": "IGV", "Built-By": System.getProperty('user.name'), "Main-Class": mainClassName, - "Class-Path": configurations.default.collect { it.getName() }.join(' ') + + "Class-Path": configurations.runtimeClasspath.collect { it.getName() }.join(' ') ) } } @@ -475,16 +452,3 @@ task fullJar(type: Jar, dependsOn: jar) { //if (!makensisCommand == "" && !signcodeCommand == "") { // dependsOn signWinExeDist //} - -// XXX: META-INF versions support (Java11) seem to clash with proguard at this point in time: -// https://sourceforge.net/p/proguard/bugs/665/ -// https://sourceforge.net/p/proguard/discussion/182456/thread/8c56b22dc2/?limit=25 -//task myproguard(type: ProGuardTask) { -//// configuration 'proguard.txt' -// -// injars "${buildDir}/IGV-dist/lib" -// outjars "${buildDir}/libs/igv-proguard.jar" -// -// //libraryjars "${buildDir}/IGV-dist/lib", jarfilter:"lib/**.jar(!META-INF/**)" -// //libraryjars "${System.getProperty('java.home')}/lib/rt.jar" -//} diff --git a/gradle/wrapper/gradle-wrapper.jar b/gradle/wrapper/gradle-wrapper.jar index 5c2d1cf016b3885f6930543d57b744ea8c220a1a..943f0cbfa754578e88a3dae77fce6e3dea56edbf 100644 GIT binary patch delta 41329 zcmaI7Q*Td!I-v?`;`vDt%y5>m*3j$(*l^jdP2JpjMA^0+&|2TRW5uH`Rl*t)xVuObX z8is+1yIO~&Kuk+s4o%X#jAkG`%UPmPu(FoL%5_`#;kGEuRVc~{{IR+C!``~k7pe0l zFXh?Sv#G{;-2u>dboTrE^TlrtNyz)gAA~dd3D%(Ez-7BcWF-3N-lU^jY(Q3BP09(v z08qAf4D0ZGh!N1O0%}ltu;LX<)c$&>15vN4OoeiB>*M_jiP3(*3DI7ybrif?aUk#2 zZ1#cK(XGztsOk*$n=#$<^-e+Pcj5{+!C$eb zN~?2cjlp%m7T~9W%6~LA1S?1-B*cW&S8#yh*9dDkSPV;;oMD(o5Ay}vOPgL_`O4c{ zc#!>?7VKF4YmW~!y6b(Dmo!%rNrLYq&g13!f`8Mups(Ds?W3 z48Eq$A>}K@X%nJ+7A53jKO5ziu79Dlo2a#`KniJ`T<~DWx3*+QhUVcXjS@OJjaRtL z%jHp$NjJ1+5c_bN<3)n;D0{)AuFf67lPOR$ zHNRwEISZtoGb>zjEKL+DOt}ycwmuCce>^IekE8o38o7Esv(qZOzvTR~n8e|*DXSG8 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b/gradle/wrapper/gradle-wrapper.properties @@ -1,5 +1,6 @@ distributionBase=GRADLE_USER_HOME distributionPath=wrapper/dists -distributionUrl=https\://services.gradle.org/distributions/gradle-5.5.1-all.zip +distributionUrl=https\://services.gradle.org/distributions/gradle-8.0.2-bin.zip +networkTimeout=10000 zipStoreBase=GRADLE_USER_HOME zipStorePath=wrapper/dists diff --git a/gradlew b/gradlew index b0d6d0ab5d..65dcd68d65 100755 --- a/gradlew +++ b/gradlew @@ -1,13 +1,13 @@ -#!/usr/bin/env sh +#!/bin/sh # -# Copyright 2015 the original author or authors. +# Copyright © 2015-2021 the original authors. # # Licensed under the Apache License, Version 2.0 (the "License"); # you may not use this file except in compliance with the License. # You may obtain a copy of the License at # -# http://www.apache.org/licenses/LICENSE-2.0 +# https://www.apache.org/licenses/LICENSE-2.0 # # Unless required by applicable law or agreed to in writing, software # distributed under the License is distributed on an "AS IS" BASIS, @@ -17,78 +17,113 @@ # ############################################################################## -## -## Gradle start up script for UN*X -## +# +# Gradle start up script for POSIX generated by Gradle. +# +# Important for running: +# +# (1) You need a POSIX-compliant shell to run this script. If your /bin/sh is +# noncompliant, but you have some other compliant shell such as ksh or +# bash, then to run this script, type that shell name before the whole +# command line, like: +# +# ksh Gradle +# +# Busybox and similar reduced shells will NOT work, because this script +# requires all of these POSIX shell features: +# * functions; +# * expansions «$var», «${var}», «${var:-default}», «${var+SET}», +# «${var#prefix}», «${var%suffix}», and «$( cmd )»; +# * compound commands having a testable exit status, especially «case»; +# * various built-in commands including «command», «set», and «ulimit». +# +# Important for patching: +# +# (2) This script targets any POSIX shell, so it avoids extensions provided +# by Bash, Ksh, etc; in particular arrays are avoided. +# +# The "traditional" practice of packing multiple parameters into a +# space-separated string is a well documented source of bugs and security +# problems, so this is (mostly) avoided, by progressively accumulating +# options in "$@", and eventually passing that to Java. +# +# Where the inherited environment variables (DEFAULT_JVM_OPTS, JAVA_OPTS, +# and GRADLE_OPTS) rely on word-splitting, this is performed explicitly; +# see the in-line comments for details. +# +# There are tweaks for specific operating systems such as AIX, CygWin, +# Darwin, MinGW, and NonStop. +# +# (3) This script is generated from the Groovy template +# https://github.com/gradle/gradle/blob/HEAD/subprojects/plugins/src/main/resources/org/gradle/api/internal/plugins/unixStartScript.txt +# within the Gradle project. +# +# You can find Gradle at https://github.com/gradle/gradle/. +# ############################################################################## # Attempt to set APP_HOME + # Resolve links: $0 may be a link -PRG="$0" -# Need this for relative symlinks. -while [ -h "$PRG" ] ; do - ls=`ls -ld "$PRG"` - link=`expr "$ls" : '.*-> \(.*\)$'` - if expr "$link" : '/.*' > /dev/null; then - PRG="$link" - else - PRG=`dirname "$PRG"`"/$link" - fi +app_path=$0 + +# Need this for daisy-chained symlinks. +while + APP_HOME=${app_path%"${app_path##*/}"} # leaves a trailing /; empty if no leading path + [ -h "$app_path" ] +do + ls=$( ls -ld "$app_path" ) + link=${ls#*' -> '} + case $link in #( + /*) app_path=$link ;; #( + *) app_path=$APP_HOME$link ;; + esac done -SAVED="`pwd`" -cd "`dirname \"$PRG\"`/" >/dev/null -APP_HOME="`pwd -P`" -cd "$SAVED" >/dev/null -APP_NAME="Gradle" -APP_BASE_NAME=`basename "$0"` +# This is normally unused +# shellcheck disable=SC2034 +APP_BASE_NAME=${0##*/} +APP_HOME=$( cd "${APP_HOME:-./}" && pwd -P ) || exit # Add default JVM options here. You can also use JAVA_OPTS and GRADLE_OPTS to pass JVM options to this script. DEFAULT_JVM_OPTS='"-Xmx64m" "-Xms64m"' # Use the maximum available, or set MAX_FD != -1 to use that value. -MAX_FD="maximum" +MAX_FD=maximum warn () { echo "$*" -} +} >&2 die () { echo echo "$*" echo exit 1 -} +} >&2 # OS specific support (must be 'true' or 'false'). cygwin=false msys=false darwin=false nonstop=false -case "`uname`" in - CYGWIN* ) - cygwin=true - ;; - Darwin* ) - darwin=true - ;; - MINGW* ) - msys=true - ;; - NONSTOP* ) - nonstop=true - ;; +case "$( uname )" in #( + CYGWIN* ) cygwin=true ;; #( + Darwin* ) darwin=true ;; #( + MSYS* | MINGW* ) msys=true ;; #( + NONSTOP* ) nonstop=true ;; esac CLASSPATH=$APP_HOME/gradle/wrapper/gradle-wrapper.jar + # Determine the Java command to use to start the JVM. if [ -n "$JAVA_HOME" ] ; then if [ -x "$JAVA_HOME/jre/sh/java" ] ; then # IBM's JDK on AIX uses strange locations for the executables - JAVACMD="$JAVA_HOME/jre/sh/java" + JAVACMD=$JAVA_HOME/jre/sh/java else - JAVACMD="$JAVA_HOME/bin/java" + JAVACMD=$JAVA_HOME/bin/java fi if [ ! -x "$JAVACMD" ] ; then die "ERROR: JAVA_HOME is set to an invalid directory: $JAVA_HOME @@ -97,7 +132,7 @@ Please set the JAVA_HOME variable in your environment to match the location of your Java installation." fi else - JAVACMD="java" + JAVACMD=java which java >/dev/null 2>&1 || die "ERROR: JAVA_HOME is not set and no 'java' command could be found in your PATH. Please set the JAVA_HOME variable in your environment to match the @@ -105,84 +140,105 @@ location of your Java installation." fi # Increase the maximum file descriptors if we can. -if [ "$cygwin" = "false" -a "$darwin" = "false" -a "$nonstop" = "false" ] ; then - MAX_FD_LIMIT=`ulimit -H -n` - if [ $? -eq 0 ] ; then - if [ "$MAX_FD" = "maximum" -o "$MAX_FD" = "max" ] ; then - MAX_FD="$MAX_FD_LIMIT" - fi - ulimit -n $MAX_FD - if [ $? -ne 0 ] ; then - warn "Could not set maximum file descriptor limit: $MAX_FD" - fi - else - warn "Could not query maximum file descriptor limit: $MAX_FD_LIMIT" - fi +if ! "$cygwin" && ! "$darwin" && ! "$nonstop" ; then + case $MAX_FD in #( + max*) + # In POSIX sh, ulimit -H is undefined. That's why the result is checked to see if it worked. + # shellcheck disable=SC3045 + MAX_FD=$( ulimit -H -n ) || + warn "Could not query maximum file descriptor limit" + esac + case $MAX_FD in #( + '' | soft) :;; #( + *) + # In POSIX sh, ulimit -n is undefined. That's why the result is checked to see if it worked. + # shellcheck disable=SC3045 + ulimit -n "$MAX_FD" || + warn "Could not set maximum file descriptor limit to $MAX_FD" + esac fi -# For Darwin, add options to specify how the application appears in the dock -if $darwin; then - GRADLE_OPTS="$GRADLE_OPTS \"-Xdock:name=$APP_NAME\" \"-Xdock:icon=$APP_HOME/media/gradle.icns\"" -fi +# Collect all arguments for the java command, stacking in reverse order: +# * args from the command line +# * the main class name +# * -classpath +# * -D...appname settings +# * --module-path (only if needed) +# * DEFAULT_JVM_OPTS, JAVA_OPTS, and GRADLE_OPTS environment variables. + +# For Cygwin or MSYS, switch paths to Windows format before running java +if "$cygwin" || "$msys" ; then + APP_HOME=$( cygpath --path --mixed "$APP_HOME" ) + CLASSPATH=$( cygpath --path --mixed "$CLASSPATH" ) + + JAVACMD=$( cygpath --unix "$JAVACMD" ) -# For Cygwin, switch paths to Windows format before running java -if $cygwin ; then - APP_HOME=`cygpath --path --mixed "$APP_HOME"` - CLASSPATH=`cygpath --path --mixed "$CLASSPATH"` - JAVACMD=`cygpath --unix "$JAVACMD"` - - # We build the pattern for arguments to be converted via cygpath - ROOTDIRSRAW=`find -L / -maxdepth 1 -mindepth 1 -type d 2>/dev/null` - SEP="" - for dir in $ROOTDIRSRAW ; do - ROOTDIRS="$ROOTDIRS$SEP$dir" - SEP="|" - done - OURCYGPATTERN="(^($ROOTDIRS))" - # Add a user-defined pattern to the cygpath arguments - if [ "$GRADLE_CYGPATTERN" != "" ] ; then - OURCYGPATTERN="$OURCYGPATTERN|($GRADLE_CYGPATTERN)" - fi # Now convert the arguments - kludge to limit ourselves to /bin/sh - i=0 - for arg in "$@" ; do - CHECK=`echo "$arg"|egrep -c "$OURCYGPATTERN" -` - CHECK2=`echo "$arg"|egrep -c "^-"` ### Determine if an option - - if [ $CHECK -ne 0 ] && [ $CHECK2 -eq 0 ] ; then ### Added a condition - eval `echo args$i`=`cygpath --path --ignore --mixed "$arg"` - else - eval `echo args$i`="\"$arg\"" + for arg do + if + case $arg in #( + -*) false ;; # don't mess with options #( + /?*) t=${arg#/} t=/${t%%/*} # looks like a POSIX filepath + [ -e "$t" ] ;; #( + *) false ;; + esac + then + arg=$( cygpath --path --ignore --mixed "$arg" ) fi - i=$((i+1)) + # Roll the args list around exactly as many times as the number of + # args, so each arg winds up back in the position where it started, but + # possibly modified. + # + # NB: a `for` loop captures its iteration list before it begins, so + # changing the positional parameters here affects neither the number of + # iterations, nor the values presented in `arg`. + shift # remove old arg + set -- "$@" "$arg" # push replacement arg done - case $i in - (0) set -- ;; - (1) set -- "$args0" ;; - (2) set -- "$args0" "$args1" ;; - (3) set -- "$args0" "$args1" "$args2" ;; - (4) set -- "$args0" "$args1" "$args2" "$args3" ;; - (5) set -- "$args0" "$args1" "$args2" "$args3" "$args4" ;; - (6) set -- "$args0" "$args1" "$args2" "$args3" "$args4" "$args5" ;; - (7) set -- "$args0" "$args1" "$args2" "$args3" "$args4" "$args5" "$args6" ;; - (8) set -- "$args0" "$args1" "$args2" "$args3" "$args4" "$args5" "$args6" "$args7" ;; - (9) set -- "$args0" "$args1" "$args2" "$args3" "$args4" "$args5" "$args6" "$args7" "$args8" ;; - esac fi -# Escape application args -save () { - for i do printf %s\\n "$i" | sed "s/'/'\\\\''/g;1s/^/'/;\$s/\$/' \\\\/" ; done - echo " " -} -APP_ARGS=$(save "$@") +# Collect all arguments for the java command; +# * $DEFAULT_JVM_OPTS, $JAVA_OPTS, and $GRADLE_OPTS can contain fragments of +# shell script including quotes and variable substitutions, so put them in +# double quotes to make sure that they get re-expanded; and +# * put everything else in single quotes, so that it's not re-expanded. + +set -- \ + "-Dorg.gradle.appname=$APP_BASE_NAME" \ + -classpath "$CLASSPATH" \ + org.gradle.wrapper.GradleWrapperMain \ + "$@" + +# Stop when "xargs" is not available. +if ! command -v xargs >/dev/null 2>&1 +then + die "xargs is not available" +fi -# Collect all arguments for the java command, following the shell quoting and substitution rules -eval set -- $DEFAULT_JVM_OPTS $JAVA_OPTS $GRADLE_OPTS "\"-Dorg.gradle.appname=$APP_BASE_NAME\"" -classpath "\"$CLASSPATH\"" org.gradle.wrapper.GradleWrapperMain "$APP_ARGS" +# Use "xargs" to parse quoted args. +# +# With -n1 it outputs one arg per line, with the quotes and backslashes removed. +# +# In Bash we could simply go: +# +# readarray ARGS < <( xargs -n1 <<<"$var" ) && +# set -- "${ARGS[@]}" "$@" +# +# but POSIX shell has neither arrays nor command substitution, so instead we +# post-process each arg (as a line of input to sed) to backslash-escape any +# character that might be a shell metacharacter, then use eval to reverse +# that process (while maintaining the separation between arguments), and wrap +# the whole thing up as a single "set" statement. +# +# This will of course break if any of these variables contains a newline or +# an unmatched quote. +# -# by default we should be in the correct project dir, but when run from Finder on Mac, the cwd is wrong -if [ "$(uname)" = "Darwin" ] && [ "$HOME" = "$PWD" ]; then - cd "$(dirname "$0")" -fi +eval "set -- $( + printf '%s\n' "$DEFAULT_JVM_OPTS $JAVA_OPTS $GRADLE_OPTS" | + xargs -n1 | + sed ' s~[^-[:alnum:]+,./:=@_]~\\&~g; ' | + tr '\n' ' ' + )" '"$@"' exec "$JAVACMD" "$@" diff --git a/gradlew.bat b/gradlew.bat index 15e1ee37a7..6689b85bee 100644 --- a/gradlew.bat +++ b/gradlew.bat @@ -5,7 +5,7 @@ @rem you may not use this file except in compliance with the License. @rem You may obtain a copy of the License at @rem -@rem http://www.apache.org/licenses/LICENSE-2.0 +@rem https://www.apache.org/licenses/LICENSE-2.0 @rem @rem Unless required by applicable law or agreed to in writing, software @rem distributed under the License is distributed on an "AS IS" BASIS, @@ -14,7 +14,7 @@ @rem limitations under the License. @rem -@if "%DEBUG%" == "" @echo off +@if "%DEBUG%"=="" @echo off @rem ########################################################################## @rem @rem Gradle startup script for Windows @@ -25,10 +25,14 @@ if "%OS%"=="Windows_NT" setlocal set DIRNAME=%~dp0 -if "%DIRNAME%" == "" set DIRNAME=. +if "%DIRNAME%"=="" set DIRNAME=. +@rem This is normally unused set APP_BASE_NAME=%~n0 set APP_HOME=%DIRNAME% +@rem Resolve any "." and ".." in APP_HOME to make it shorter. +for %%i in ("%APP_HOME%") do set APP_HOME=%%~fi + @rem Add default JVM options here. You can also use JAVA_OPTS and GRADLE_OPTS to pass JVM options to this script. set DEFAULT_JVM_OPTS="-Xmx64m" "-Xms64m" @@ -37,7 +41,7 @@ if defined JAVA_HOME goto findJavaFromJavaHome set JAVA_EXE=java.exe %JAVA_EXE% -version >NUL 2>&1 -if "%ERRORLEVEL%" == "0" goto init +if %ERRORLEVEL% equ 0 goto execute echo. echo ERROR: JAVA_HOME is not set and no 'java' command could be found in your PATH. @@ -51,7 +55,7 @@ goto fail set JAVA_HOME=%JAVA_HOME:"=% set JAVA_EXE=%JAVA_HOME%/bin/java.exe -if exist "%JAVA_EXE%" goto init +if exist "%JAVA_EXE%" goto execute echo. echo ERROR: JAVA_HOME is set to an invalid directory: %JAVA_HOME% @@ -61,38 +65,26 @@ echo location of your Java installation. goto fail -:init -@rem Get command-line arguments, handling Windows variants - -if not "%OS%" == "Windows_NT" goto win9xME_args - -:win9xME_args -@rem Slurp the command line arguments. -set CMD_LINE_ARGS= -set _SKIP=2 - -:win9xME_args_slurp -if "x%~1" == "x" goto execute - -set CMD_LINE_ARGS=%* - :execute @rem Setup the command line set CLASSPATH=%APP_HOME%\gradle\wrapper\gradle-wrapper.jar + @rem Execute Gradle -"%JAVA_EXE%" %DEFAULT_JVM_OPTS% %JAVA_OPTS% %GRADLE_OPTS% "-Dorg.gradle.appname=%APP_BASE_NAME%" -classpath "%CLASSPATH%" org.gradle.wrapper.GradleWrapperMain %CMD_LINE_ARGS% +"%JAVA_EXE%" %DEFAULT_JVM_OPTS% %JAVA_OPTS% %GRADLE_OPTS% "-Dorg.gradle.appname=%APP_BASE_NAME%" -classpath "%CLASSPATH%" org.gradle.wrapper.GradleWrapperMain %* :end @rem End local scope for the variables with windows NT shell -if "%ERRORLEVEL%"=="0" goto mainEnd +if %ERRORLEVEL% equ 0 goto mainEnd :fail rem Set variable GRADLE_EXIT_CONSOLE if you need the _script_ return code instead of rem the _cmd.exe /c_ return code! -if not "" == "%GRADLE_EXIT_CONSOLE%" exit 1 -exit /b 1 +set EXIT_CODE=%ERRORLEVEL% +if %EXIT_CODE% equ 0 set EXIT_CODE=1 +if not ""=="%GRADLE_EXIT_CONSOLE%" exit %EXIT_CODE% +exit /b %EXIT_CODE% :mainEnd if "%OS%"=="Windows_NT" endlocal diff --git a/scripts/test_suite.args b/scripts/test_suite.args deleted file mode 100644 index 6331a3cfcb..0000000000 --- a/scripts/test_suite.args +++ /dev/null @@ -1,75 +0,0 @@ -# Arguments to support running the test suite on Java 9 and 10 -# Need to expose internals to junit for testing. - ---add-exports org.igv/org.broad.igv.annotations=junit ---add-exports org.igv/org.broad.igv.batch=junit ---add-exports org.igv/org.broad.igv.bbfile=junit ---add-exports org.igv/org.broad.igv.bigwig=junit ---add-exports org.igv/org.broad.igv.charts=junit ---add-exports org.igv/org.broad.igv.cli_plugin.ui=junit ---add-exports org.igv/org.broad.igv.data=junit ---add-exports org.igv/org.broad.igv.data.cufflinks=junit ---add-exports org.igv/org.broad.igv.data.expression=junit ---add-exports org.igv/org.broad.igv.data.seg=junit ---add-exports org.igv/org.broad.igv.event=junit ---add-exports org.igv/org.broad.igv.exceptions=junit ---add-exports org.igv/org.broad.igv.feature=junit ---add-exports org.igv/org.broad.igv.feature.aa=junit ---add-exports org.igv/org.broad.igv.feature.basepair=junit ---add-exports org.igv/org.broad.igv.feature.bionano=junit ---add-exports org.igv/org.broad.igv.feature.dranger=junit ---add-exports org.igv/org.broad.igv.feature.dsi=junit ---add-exports org.igv/org.broad.igv.feature.genome=junit ---add-exports org.igv/org.broad.igv.feature.genome.fasta=junit ---add-exports org.igv/org.broad.igv.feature.tribble=junit ---add-exports org.igv/org.broad.igv.feature.tribble.reader=junit ---add-exports org.igv/org.broad.igv.oauth=junit ---add-exports org.igv/org.broad.igv.gwas=junit ---add-exports org.igv/org.broad.igv.lists=junit ---add-exports org.igv/org.broad.igv.maf=junit ---add-exports org.igv/org.broad.igv.methyl=junit ---add-exports org.igv/org.broad.igv.peaks=junit ---add-exports org.igv/org.broad.igv.prefs=junit ---add-exports org.igv/org.broad.igv.repeats=junit ---add-exports org.igv/org.broad.igv.renderer=junit ---add-exports org.igv/org.broad.igv.sam=junit ---add-exports org.igv/org.broad.igv.sam.mods=junit ---add-exports org.igv/org.broad.igv.sam.cram=junit ---add-exports org.igv/org.broad.igv.sam.reader=junit ---add-exports org.igv/org.broad.igv.session=junit ---add-exports org.igv/org.broad.igv.tdf=junit ---add-exports org.igv/org.broad.igv.tools.converters=junit ---add-exports org.igv/org.broad.igv.tools.motiffinder=junit ---add-exports org.igv/org.broad.igv.tools.parsers=junit ---add-exports org.igv/org.broad.igv.tools.sort=junit ---add-exports org.igv/org.broad.igv.tools.ui=junit ---add-exports org.igv/org.broad.igv.track=junit ---add-exports org.igv/org.broad.igv.ui.action=junit ---add-exports org.igv/org.broad.igv.ui.color=junit ---add-exports org.igv/org.broad.igv.ui.commandbar=junit ---add-exports org.igv/org.broad.igv.ui.dnd=junit ---add-exports org.igv/org.broad.igv.ui.filefilters=junit ---add-exports org.igv/org.broad.igv.ui.legend=junit ---add-exports org.igv/org.broad.igv.ui.panel=junit ---add-exports org.igv/org.broad.igv.ui.svg=junit ---add-exports org.igv/org.broad.igv.ui.util=junit ---add-exports org.igv/org.broad.igv.ui.util.download=junit ---add-exports org.igv/org.broad.igv.util=junit ---add-exports org.igv/org.broad.igv.util.blat=junit ---add-exports org.igv/org.broad.igv.util.collections=junit ---add-exports org.igv/org.broad.igv.util.converters=junit ---add-exports org.igv/org.broad.igv.util.extview=junit ---add-exports org.igv/org.broad.igv.util.encode=junit ---add-exports org.igv/org.broad.igv.util.ftp=junit ---add-exports org.igv/org.broad.igv.util.index=junit ---add-exports org.igv/org.broad.igv.util.stream=junit ---add-exports org.igv/org.broad.igv.util.stats=junit ---add-exports org.igv/org.broad.igv.variant=junit ---add-exports org.igv/org.broad.igv.variant.New=junit ---add-exports org.igv/org.broad.igv.variant.util=junit ---add-exports org.igv/org.broad.igv.variant.vcf=junit ---add-exports org.igv/biz.source_code.base64Coder=junit ---add-exports org.igv/org.broad.igv.preprocess=junit ---add-exports org.igv/org.broad.tribble=junit ---add-exports org.igv/util=junit ---add-exports org.igv/org.broad.igv.htsget=junit diff --git a/src/main/java/com/sanityinc/jargs/CmdLineParser.java b/src/main/java/com/sanityinc/jargs/CmdLineParser.java index 970eb00aea..f45c7364c6 100644 --- a/src/main/java/com/sanityinc/jargs/CmdLineParser.java +++ b/src/main/java/com/sanityinc/jargs/CmdLineParser.java @@ -181,7 +181,7 @@ protected Option( String longForm, boolean wantsValue ) { protected Option( char shortForm, String longForm, boolean wantsValue ) { - this(new String(new char[]{shortForm}), longForm, wantsValue); + this(String.valueOf(shortForm), longForm, wantsValue); } private Option( String shortForm, String longForm, boolean wantsValue ) { @@ -281,7 +281,7 @@ public IntegerOption( String longForm ) { protected Integer parseValue( String arg, Locale locale ) throws IllegalOptionValueException { try { - return new Integer(arg); + return Integer.valueOf(arg); } catch (NumberFormatException e) { throw new IllegalOptionValueException(this, arg); } @@ -303,7 +303,7 @@ public LongOption( String longForm ) { protected Long parseValue( String arg, Locale locale ) throws IllegalOptionValueException { try { - return new Long(arg); + return Long.valueOf(arg); } catch (NumberFormatException e) { throw new IllegalOptionValueException(this, arg); } diff --git a/src/main/java/org/broad/igv/feature/genome/Genome.java b/src/main/java/org/broad/igv/feature/genome/Genome.java index 6d96bd4b62..afa928f6b9 100644 --- a/src/main/java/org/broad/igv/feature/genome/Genome.java +++ b/src/main/java/org/broad/igv/feature/genome/Genome.java @@ -334,10 +334,10 @@ public long getCumulativeOffset(String chr) { } offset += getChromosome(c).getLength(); } - cumOffset = new Long(offset); + cumOffset = offset; cumulativeOffsets.put(chr, cumOffset); } - return cumOffset.longValue(); + return cumOffset; } /** diff --git a/src/main/java/org/broad/igv/prefs/IGVPreferences.java b/src/main/java/org/broad/igv/prefs/IGVPreferences.java index 61bf5db497..96d170f034 100644 --- a/src/main/java/org/broad/igv/prefs/IGVPreferences.java +++ b/src/main/java/org/broad/igv/prefs/IGVPreferences.java @@ -1,3 +1,4 @@ + /* * The MIT License (MIT) * @@ -73,9 +74,9 @@ public class IGVPreferences { Set overrideKeys = new HashSet<>(); // Cached non-string preference values - private Map booleanCache = new Hashtable(); - private Map objectCache = new Hashtable(); - private Map colorScaleCache = new Hashtable(); + private Map booleanCache = new Hashtable<>(); + private Map objectCache = new Hashtable<>(); + private Map colorScaleCache = new Hashtable<>(); private PaletteColorTable mutationColorScheme = null; public IGVPreferences() { @@ -141,7 +142,7 @@ public boolean getAsBoolean(String key) { boolValue = Boolean.valueOf(get(key, value)); booleanCache.put(key, boolValue); } - return boolValue.booleanValue(); + return boolValue; } /** @@ -159,7 +160,7 @@ public int getAsInt(String key) { log.warn("No default value for: " + key); return 0; } - value = new Integer(get(key, defValue)); + value = Integer.valueOf(get(key, defValue)); objectCache.put(key, value); } return value.intValue(); @@ -201,7 +202,7 @@ public float getAsFloat(String key) { log.warn("No default value for: " + key); return 0; } - value = new Float(get(key, defValue)); + value = Float.valueOf(get(key, defValue)); objectCache.put(key, value); } return value.floatValue(); @@ -235,7 +236,7 @@ public void addOverrides(Map newPrefs) { public boolean getAntiAliasing() { - if (userPreferences.containsKey(Constants.ENABLE_ANTIALISING) || Globals.IS_LINUX == false) { + if (userPreferences.containsKey(Constants.ENABLE_ANTIALISING) || !Globals.IS_LINUX) { return getAsBoolean(Constants.ENABLE_ANTIALISING); } else { // Linux with no explicit setting @@ -252,9 +253,9 @@ public boolean getAntiAliasing() { private void updateCaches(String key, String value) { key = key.trim(); if (booleanCache.containsKey(key)) { - booleanCache.put(key, new Boolean(value)); + booleanCache.put(key, Boolean.valueOf(value)); } - colorScaleCache.remove(key); + colorScaleCache.remove(key); //TODO color scale cache doesn't use key strings so something here is wrong. objectCache.remove(key); mutationColorScheme = null; } @@ -417,7 +418,7 @@ public void remove(String key) { userPreferences.remove(key); booleanCache.remove(key); objectCache.remove(key); - colorScaleCache.remove(key); + colorScaleCache.remove(key); //TODO same issue of cache not using String keys IGVEventBus.getInstance().post(new PreferencesChangeEvent()); } diff --git a/src/main/java/org/broad/igv/sam/BaseAlignmentCounts.java b/src/main/java/org/broad/igv/sam/BaseAlignmentCounts.java index 936ac7a653..36a82af7f0 100644 --- a/src/main/java/org/broad/igv/sam/BaseAlignmentCounts.java +++ b/src/main/java/org/broad/igv/sam/BaseAlignmentCounts.java @@ -280,27 +280,19 @@ private static void loadKnownSnps(String snpFile) { return; } - knownSnps = new HashMap(); - AsciiLineReader reader = null; - try { - reader = ParsingUtils.openAsciiReader(new ResourceLocator(snpFile)); + knownSnps = new HashMap<>(); + try (AsciiLineReader reader = ParsingUtils.openAsciiReader(new ResourceLocator(snpFile))) { String nextLine = ""; while ((nextLine = reader.readLine()) != null) { String[] tokens = nextLine.split("\t"); String chr = tokens[0]; - Set snps = knownSnps.get(chr); - if (snps == null) { - snps = new HashSet(10000); - knownSnps.put(chr, snps); - } - snps.add(new Integer(tokens[1])); + Set snps = knownSnps.computeIfAbsent(chr, k -> new HashSet<>(10000)); + snps.add(Integer.valueOf(tokens[1])); } } catch (Exception e) { knownSnps = null; log.error("", e); MessageUtils.showMessage("Error loading snps file: " + snpFile + " (" + e.toString() + ")"); - } finally { - reader.close(); } diff --git a/src/main/java/org/broad/igv/sam/SparseAlignmentCounts.java b/src/main/java/org/broad/igv/sam/SparseAlignmentCounts.java index 02de430601..7bfb7f5be6 100644 --- a/src/main/java/org/broad/igv/sam/SparseAlignmentCounts.java +++ b/src/main/java/org/broad/igv/sam/SparseAlignmentCounts.java @@ -439,12 +439,7 @@ protected void incPositionCount(int pos, byte b, byte q, boolean isNegativeStran } private int getIndex(int pos) { - Integer index = indexMap.get(pos); - if (index == null) { - index = new Integer(indexMap.size()); - indexMap.put(pos, index); - } - return index.intValue(); + return indexMap.computeIfAbsent(pos, k -> indexMap.size()); } diff --git a/src/main/java/org/broad/igv/sam/reader/BAMReader.java b/src/main/java/org/broad/igv/sam/reader/BAMReader.java index 1addd6b6a7..9765483e02 100644 --- a/src/main/java/org/broad/igv/sam/reader/BAMReader.java +++ b/src/main/java/org/broad/igv/sam/reader/BAMReader.java @@ -119,7 +119,7 @@ private void loadSequenceDictionary() { if (records.size() > 0) { for (SAMSequenceRecord rec : header.getSequenceDictionary().getSequences()) { String chr = rec.getSequenceName(); - Long size = new Long(rec.getSequenceLength()); + Long size = (long) rec.getSequenceLength(); sequenceNames.add(chr); sequenceDictionary.put(chr, size); diff --git a/src/main/java/org/broad/igv/session/IGVSessionReader.java b/src/main/java/org/broad/igv/session/IGVSessionReader.java index 7a4f750b0a..c4483c780b 100644 --- a/src/main/java/org/broad/igv/session/IGVSessionReader.java +++ b/src/main/java/org/broad/igv/session/IGVSessionReader.java @@ -307,7 +307,7 @@ private void addLeftoverTracks(Collection> tmp) { //For resetting track panels later List> trackPanelAttrs = null; trackPanelAttrs = igv.getTrackPanelAttrs(); - + for (List tracks : tmp) { for (Track track : tracks) { if (track == geneTrack) { @@ -536,10 +536,10 @@ void processResource(Session session, Element element, String sessionPath) { // URL to a database or webservice -- this is not common String serverURL = getAttribute(element, SessionAttribute.SERVER_URL); - + String path = getAttribute(element, SessionAttribute.PATH); - // Older sessions used the "name" attribute for the path. + // Older sessions used the "name" attribute for the path. if (oldSession && name != null) { path = name; int idx = name.lastIndexOf("/"); @@ -630,7 +630,7 @@ private void processRegion(Session session, Element element, String sessionPath) String end = getAttribute(element, SessionAttribute.END_INDEX); String description = getAttribute(element, SessionAttribute.DESCRIPTION); - RegionOfInterest region = new RegionOfInterest(chromosome, new Integer(start), new Integer(end), description); + RegionOfInterest region = new RegionOfInterest(chromosome, Integer.parseInt(start), Integer.parseInt(end), description); igv.addRegionOfInterest(region); NodeList elements = element.getChildNodes(); diff --git a/src/main/java/org/broad/igv/ui/action/MenuAction.java b/src/main/java/org/broad/igv/ui/action/MenuAction.java index 16fd3c51da..dc28269a92 100644 --- a/src/main/java/org/broad/igv/ui/action/MenuAction.java +++ b/src/main/java/org/broad/igv/ui/action/MenuAction.java @@ -50,7 +50,7 @@ public class MenuAction extends AbstractAction { public MenuAction(String name, Icon icon, int mnemonic) { super(name, icon); if(mnemonic >= 0) { - putValue(MNEMONIC_KEY, new Integer(mnemonic)); + putValue(MNEMONIC_KEY, mnemonic); } } diff --git a/src/main/java/org/broad/igv/util/Utilities.java b/src/main/java/org/broad/igv/util/Utilities.java index c5c4a997ad..570900eeaa 100644 --- a/src/main/java/org/broad/igv/util/Utilities.java +++ b/src/main/java/org/broad/igv/util/Utilities.java @@ -164,7 +164,7 @@ public static String getNullSafe(NamedNodeMap attr, String key) { static public Comparator getNumericStringComparator() { - Comparator comparator = new Comparator() { + Comparator comparator = new Comparator() { public int compare(String s1, String s2) { StringTokenizer st1 = new StringTokenizer(s1, " "); @@ -177,10 +177,10 @@ public int compare(String s1, String s2) { int c; try { - Integer i1 = new Integer(t1); - Integer i2 = new Integer(t2); + int i1 = Integer.parseInt(t1); + int i2 = Integer.parseInt(t2); - c = i1.compareTo(i2); + c = Integer.compare(i1, i2); } catch (NumberFormatException e) { c = t1.compareTo(t2); }

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