These lessons are adapted from a tutorial presented by the HBC training team at the 2018 GCC/BOSC conference. The adaptation is for a 3 hour presentation about RNA-seq (bulk & single cell) experimental design for CELLBIO 321qc.
Instructor 1: Dr. Sarah Boswell, Laboratory of Systems Pharmacology, Single Cell Core
Instructor 2: Dr. Radhika Khetani, Training Director at the Harvard Chan Bioinformatics Core
Contributors: Dr. Mary Piper and Dr. Meeta Mistry, Harvard Chan Bioinformatics Core
| Time | Topic | Instructor |
|---|---|---|
| 9:00 - 9:10 | Introduction to module | Radhika & Sarah |
| 9:10 - 9:25 | Sequencing by synthesis | Sarah |
| 9:25 - 9:50 | Experimental design for RNA-seq | Radhika |
| 9:50 - 10:35 | RNA-seq library preparation | Sarah |
| 10:35 - 10:45 | Break | |
| 10:45 - 11:05 | Practical strategies for sequencing | Radhika & Sarah |
| 11:05 - 11:40 | Analysis workflow and tools (bulk RNA-seq) | Radhika |
| 11:40 - 11:50 | Analysis workflow and tools (single-cell RNA-seq) | Radhika |
| 11:50 - 12:00 | Wrap-up | Sarah & Radhika |