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Request optimized default alignment parameters for ONT data #21

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seboyden opened this issue Dec 18, 2024 · 1 comment
Closed

Request optimized default alignment parameters for ONT data #21

seboyden opened this issue Dec 18, 2024 · 1 comment

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@seboyden
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The default settings for --alignment-params are optimized for PacBio HiFi reads. You suggest increasing the gap-open values f and g for ONT reads with a higher indel error rate, but it's not clear what appropriate values would be. Would be great to have a set of optimized ONT default values.

@heliziii
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Hello. Thank you for using LongTR. I tested with values -1 for f and g instead of -10 and alignments seemed more accurate, but we did not systematically test different values. Please let me know if you have any other questions.

Best,
Helia

@heliziii heliziii closed this as completed Jan 5, 2025
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