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An error in L-GIREMI #4

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bxZhang996 opened this issue Jul 19, 2024 · 0 comments
Open

An error in L-GIREMI #4

bxZhang996 opened this issue Jul 19, 2024 · 0 comments

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@bxZhang996
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Dear Zhiheng,
I hope to discover RNA edit events in the long-read RNA-seq of chickens. But I encountered the following error while running L-GIREMI:

##############################################################
multiprocessing.pool.RemoteTraceback:
"""
Traceback (most recent call last):
File "/BIGDATA2/cau_clwen_1/.conda/envs/xuan310/lib/python3.10/multiprocessing/pool.py", line 125, in worker
result = (True, func(*args, **kwds))
File "/BIGDATA2/cau_clwen_1/.conda/envs/xuan310/lib/python3.10/multiprocessing/pool.py", line 48, in mapstar
return list(map(*args))
File "/BIGDATA2/cau_clwen_1/.conda/envs/xuan310/lib/python3.10/site-packages/l_giremi-0.2.3-py3.10.egg/EGG-INFO/scripts/l-giremi", line 35, in footprint_bulk_calculation
File "/BIGDATA2/cau_clwen_1/.conda/envs/xuan310/lib/python3.10/site-packages/l_giremi-0.2.3-py3.10.egg/giremi/strand.py", line 191, in correct_read_strand_in_region
gtf_entry.gene_name,
File "pysam/libctabixproxies.pyx", line 638, in pysam.libctabixproxies.GTFProxy.getattr
KeyError: 'gene_name'
"""
The above exception was the direct cause of the following exception:
Traceback (most recent call last):
File "/BIGDATA2/cau_clwen_1/.conda/envs/xuan310/bin/l-giremi", line 4, in
import('pkg_resources').run_script('l-giremi==0.2.3', 'l-giremi')
File "/BIGDATA2/cau_clwen_1/.conda/envs/xuan310/lib/python3.10/site-packages/pkg_resources/init.py", line 672, in run_script
self.require(requires)[0].run_script(script_name, ns)
File "/BIGDATA2/cau_clwen_1/.conda/envs/xuan310/lib/python3.10/site-packages/pkg_resources/init.py", line 1479, in run_script
exec(script_code, namespace, namespace)
File "/BIGDATA2/cau_clwen_1/.conda/envs/xuan310/lib/python3.10/site-packages/l_giremi-0.2.3-py3.10.egg/EGG-INFO/scripts/l-giremi", line 371, in
File "/BIGDATA2/cau_clwen_1/.conda/envs/xuan310/lib/python3.10/multiprocessing/pool.py", line 364, in map
return self._map_async(func, iterable, mapstar, chunksize).get()
File "/BIGDATA2/cau_clwen_1/.conda/envs/xuan310/lib/python3.10/multiprocessing/pool.py", line 771, in get
raise self._value
KeyError: 'gene_name'
##############################################################

It seems that there is an issue with my reference GTF. The GTF annotation file (Gallus_gallus.GRCg6a.106.gtf) which downloaded from ensembl , I found that there are indeed some transcripts or genes that do not have annotation information for 'gene_name'. Do you have any suggestions for this KeyError?

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