diff --git a/docs/migration/v8-to-v9.md b/docs/migration/v8-to-v9.md
index 6206c60cbb1f..ae2f68bb0e38 100644
--- a/docs/migration/v8-to-v9.md
+++ b/docs/migration/v8-to-v9.md
@@ -98,6 +98,19 @@ It will be removed in a future major version.
 
 - The `debugIntegration` has been removed. To log outgoing events, use [Hook Options](https://docs.sentry.io/platforms/javascript/configuration/options/#hooks) (`beforeSend`, `beforeSendTransaction`, ...).
 - The `sessionTimingIntegration` has been removed. To capture session durations alongside events, use [Context](https://docs.sentry.io/platforms/javascript/enriching-events/context/) (`Sentry.setContext()`).
+- The `addOpenTelemetryInstrumentation` method has been removed. Use the `openTelemetryInstrumentations` option in `Sentry.init()` or your custom Sentry Client instead.
+
+```js
+import * as Sentry from '@sentry/node';
+
+// before
+Sentry.addOpenTelemetryInstrumentation(new GenericPoolInstrumentation());
+
+// after
+Sentry.init({
+  openTelemetryInstrumentations: [new GenericPoolInstrumentation()],
+});
+```
 
 ### `@sentry/react`
 
diff --git a/packages/astro/src/index.server.ts b/packages/astro/src/index.server.ts
index c5f3f74699d6..9152f67abf62 100644
--- a/packages/astro/src/index.server.ts
+++ b/packages/astro/src/index.server.ts
@@ -12,8 +12,6 @@ export {
   addEventProcessor,
   addIntegration,
   // eslint-disable-next-line deprecation/deprecation
-  addOpenTelemetryInstrumentation,
-  // eslint-disable-next-line deprecation/deprecation
   addRequestDataToEvent,
   amqplibIntegration,
   anrIntegration,
diff --git a/packages/aws-serverless/src/index.ts b/packages/aws-serverless/src/index.ts
index c50d796415e9..c40266ab59a9 100644
--- a/packages/aws-serverless/src/index.ts
+++ b/packages/aws-serverless/src/index.ts
@@ -114,8 +114,6 @@ export {
   spanToBaggageHeader,
   trpcMiddleware,
   updateSpanName,
-  // eslint-disable-next-line deprecation/deprecation
-  addOpenTelemetryInstrumentation,
   zodErrorsIntegration,
   profiler,
   amqplibIntegration,
diff --git a/packages/bun/src/index.ts b/packages/bun/src/index.ts
index 6b172c998d64..0d476efd910b 100644
--- a/packages/bun/src/index.ts
+++ b/packages/bun/src/index.ts
@@ -136,8 +136,6 @@ export {
   spanToBaggageHeader,
   trpcMiddleware,
   updateSpanName,
-  // eslint-disable-next-line deprecation/deprecation
-  addOpenTelemetryInstrumentation,
   zodErrorsIntegration,
   profiler,
   amqplibIntegration,
diff --git a/packages/google-cloud-serverless/src/index.ts b/packages/google-cloud-serverless/src/index.ts
index 2c04193da8f3..ace6ff127c3d 100644
--- a/packages/google-cloud-serverless/src/index.ts
+++ b/packages/google-cloud-serverless/src/index.ts
@@ -113,8 +113,6 @@ export {
   spanToBaggageHeader,
   trpcMiddleware,
   updateSpanName,
-  // eslint-disable-next-line deprecation/deprecation
-  addOpenTelemetryInstrumentation,
   zodErrorsIntegration,
   profiler,
   amqplibIntegration,
diff --git a/packages/node/src/index.ts b/packages/node/src/index.ts
index b4c556c39f81..bd2ae0722f87 100644
--- a/packages/node/src/index.ts
+++ b/packages/node/src/index.ts
@@ -58,8 +58,6 @@ export type { NodeOptions } from './types';
 export { addRequestDataToEvent, DEFAULT_USER_INCLUDES, extractRequestData } from '@sentry/core';
 
 export {
-  // eslint-disable-next-line deprecation/deprecation
-  addOpenTelemetryInstrumentation,
   // These are custom variants that need to be used instead of the core one
   // As they have slightly different implementations
   continueTrace,
diff --git a/packages/opentelemetry/src/index.ts b/packages/opentelemetry/src/index.ts
index 30228d0dd763..ff8ec1655edd 100644
--- a/packages/opentelemetry/src/index.ts
+++ b/packages/opentelemetry/src/index.ts
@@ -58,8 +58,5 @@ export {
 
 export { openTelemetrySetupCheck } from './utils/setupCheck';
 
-// eslint-disable-next-line deprecation/deprecation
-export { addOpenTelemetryInstrumentation } from './instrumentation';
-
 // Legacy
 export { getClient } from '@sentry/core';
diff --git a/packages/opentelemetry/src/instrumentation.ts b/packages/opentelemetry/src/instrumentation.ts
deleted file mode 100644
index 979282d0e467..000000000000
--- a/packages/opentelemetry/src/instrumentation.ts
+++ /dev/null
@@ -1,15 +0,0 @@
-import type { Instrumentation } from '@opentelemetry/instrumentation';
-import { registerInstrumentations } from '@opentelemetry/instrumentation';
-
-/**
- * This method takes an OpenTelemetry instrumentation or
- * array of instrumentations and registers them with OpenTelemetry.
- *
- * @deprecated This method will be removed in the next major version of the SDK.
- * Use the `openTelemetryInstrumentations` option in `Sentry.init()` or your custom Sentry Client instead.
- */
-export function addOpenTelemetryInstrumentation(...instrumentations: Instrumentation[]): void {
-  registerInstrumentations({
-    instrumentations,
-  });
-}
diff --git a/packages/remix/src/index.server.ts b/packages/remix/src/index.server.ts
index 109e76d7053f..193cbd072b39 100644
--- a/packages/remix/src/index.server.ts
+++ b/packages/remix/src/index.server.ts
@@ -16,8 +16,6 @@ export {
   addEventProcessor,
   addIntegration,
   // eslint-disable-next-line deprecation/deprecation
-  addOpenTelemetryInstrumentation,
-  // eslint-disable-next-line deprecation/deprecation
   addRequestDataToEvent,
   amqplibIntegration,
   anrIntegration,
diff --git a/packages/solidstart/src/server/index.ts b/packages/solidstart/src/server/index.ts
index b0554463f427..4963104f7d4e 100644
--- a/packages/solidstart/src/server/index.ts
+++ b/packages/solidstart/src/server/index.ts
@@ -8,8 +8,6 @@ export {
   addEventProcessor,
   addIntegration,
   // eslint-disable-next-line deprecation/deprecation
-  addOpenTelemetryInstrumentation,
-  // eslint-disable-next-line deprecation/deprecation
   addRequestDataToEvent,
   amqplibIntegration,
   anrIntegration,
diff --git a/packages/sveltekit/src/server/index.ts b/packages/sveltekit/src/server/index.ts
index ff31b715768f..cbd4934744bf 100644
--- a/packages/sveltekit/src/server/index.ts
+++ b/packages/sveltekit/src/server/index.ts
@@ -8,8 +8,6 @@ export {
   addEventProcessor,
   addIntegration,
   // eslint-disable-next-line deprecation/deprecation
-  addOpenTelemetryInstrumentation,
-  // eslint-disable-next-line deprecation/deprecation
   addRequestDataToEvent,
   amqplibIntegration,
   anrIntegration,