workshop-python_pandas_gnmk.ipynb

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    "<a href=\"https://colab.research.google.com/github/genomika/pandas-workshop/blob/master/workshop-python_pandas_gnmk.ipynb\" target=\"_parent\"><img src=\"https://colab.research.google.com/assets/colab-badge.svg\" alt=\"Open In Colab\"/></a>"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {
    "colab_type": "text",
    "id": "73K30GnTmIsV"
   },
   "source": [
    "# I Workshop de introdução a lib Pandas\n",
    "\n",
    "![pandas](https://files.realpython.com/media/Python-Pandas-10-Tricks--Features-You-May-Not-Know-Watermark.e58bb5ce9835.jpg)\n",
    "Figura 1: [\"Python Pandas: Tricks & Features You May Not Know\"](https://realpython.com/python-pandas-tricks/)\n",
    "\n",
    "0. Instalando conda, jupyter notebook, pandas, etc;\n",
    "\n",
    "  * [Instalando Conda no Ubuntu 18.04](https://www.digitalocean.com/community/tutorials/how-to-install-anaconda-on-ubuntu-18-04-quickstart-pt)\n",
    "  * [Instalando Conda no Windows](https://docs.conda.io/projects/conda/en/latest/user-guide/install/windows.html)\n",
    "  * [Instalando Conda no MacOS](https://docs.conda.io/projects/conda/en/latest/user-guide/install/macos.html)\n",
    "\n",
    "1. Abrindo arquivos com Pandas\n",
    "\n",
    "  * Series e DataFrames;\n",
    "  * CSV;\n",
    "  * Excel;\n",
    "  * Python dicionários;\n",
    "  * Python lista de tuplas;\n",
    "  * Python lista de dicionários;\n",
    "  * Manipulação básica de `DataFrame`s;\n",
    "  * Escrevendo CSV, Excel.\n",
    "\n",
    "2. Manipulando `DataFrame`s\n",
    "  \n",
    "  * Manipulando índices;\n",
    "  * Filtrando `DataFrame`s;\n",
    "  * Trabalhando com dados faltantes;\n",
    "  * Agrupando um `DataFrame`;\n",
    "  * Como juntar diferentes `DataFrame`s.\n",
    "\n",
    "3. Manipulando big data com Vaex\n",
    "\n",
    "4. Hands on com amostra de exemplo"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {
    "colab_type": "text",
    "id": "3LdWowTJNdYz"
   },
   "source": [
    "# 1. Abrindo arquivos com Pandas"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {
    "colab_type": "text",
    "id": "HydL_GNymqk7"
   },
   "source": [
    "### `Series` e `DataFrame`\n",
    "\n",
    "Para trabalhar manipulando dados com pandas é necessário importar esses dados de alguma forma para dentro da biblioteca. A forma mais comum de atingir esse objetivo é criar, usando o material de análise, estruturas de dados nativas do framework. Nesse curso, trabalharemos essencialmente com [*`DataFrame`*](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.DataFrame.html) e [*`Series`*](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.Series.html).\n",
    "\n",
    "`Series` são essencialmente uma estrutura similar a arrays unidimensionais cujo objetivo é guardar uma sequência de valores de forma indexada."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 2,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": "não        42\naqui       13\né o         7\npatrick   -99\ndtype: int64"
     },
     "execution_count": 2,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "import pandas as pd\n",
    "series = pd.Series([42, 13, 7, -99], index=[\"não\", \"aqui\", \"é o\", \"patrick\"])\n",
    "series"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {
    "colab_type": "text",
    "id": "YYgh-O3hdbJh"
   },
   "source": [
    "De maneira mais simples, podemos interpretar `Series` como dicionários ordenados e de tamanho fixo. Inclusive, conseguimos criar essas estruturas a partir de dicionários, e usá-las em contextos onde usaríamos dicionários."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 2,
   "metadata": {
    "colab": {},
    "colab_type": "code",
    "id": "KM_UV4krlUth"
   },
   "outputs": [
    {
     "data": {
      "text/plain": [
       "Shape of You         2433000\n",
       "Rockstar             1831000\n",
       "One Dance            1817000\n",
       "Closer               1724000\n",
       "Thinking Out Loud    1493000\n",
       "dtype: int64"
      ]
     },
     "execution_count": 2,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# https://en.wikipedia.org/wiki/List_of_most-streamed_songs_on_Spotify\n",
    "spotify_mais_escutadas = {\"Shape of You\": 2433000, \"Rockstar\": 1831000, \"One Dance\": 1817000, \"Closer\": 1724000, \"Thinking Out Loud\": 1493000}\n",
    "mais_escutadas_series = pd.Series(spotify_mais_escutadas)\n",
    "mais_escutadas_series"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 3,
   "metadata": {
    "colab": {},
    "colab_type": "code",
    "id": "pyxLG_f8sGyI"
   },
   "outputs": [
    {
     "data": {
      "text/plain": [
       "True"
      ]
     },
     "execution_count": 3,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "\"Rockstar\" in mais_escutadas_series"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 4,
   "metadata": {
    "colab": {},
    "colab_type": "code",
    "id": "1sappD52sR2i"
   },
   "outputs": [
    {
     "data": {
      "text/plain": [
       "False"
      ]
     },
     "execution_count": 4,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "\"Bota Bota\" in mais_escutadas_series # :("
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {
    "colab_type": "text",
    "id": "qjRWIVCNv95y"
   },
   "source": [
    "`DataFrame`s são representações tabulares de dados. Essa estrutura contém um conjunto ordenado de colunas, podendo cada uma ter um tipo especíco de valor (numérico, texto, boleano e etc.). No `DataFrame` ambas colunas e linhas possuem índice."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 5,
   "metadata": {
    "colab": {},
    "colab_type": "code",
    "id": "S9mMB9FR0vOI"
   },
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>música</th>\n",
       "      <th>número_streams</th>\n",
       "      <th>data_publicação</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>Shape of You</td>\n",
       "      <td>2433000</td>\n",
       "      <td>2017-01-06</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>Rockstar</td>\n",
       "      <td>1831000</td>\n",
       "      <td>2017-09-15</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>One Dance</td>\n",
       "      <td>1817000</td>\n",
       "      <td>2016-04-05</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>Closer</td>\n",
       "      <td>1724000</td>\n",
       "      <td>2016-07-29</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>Thinking Out Loud</td>\n",
       "      <td>1493000</td>\n",
       "      <td>2014-06-20</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "</div>"
      ],
      "text/plain": [
       "              música  número_streams data_publicação\n",
       "0       Shape of You         2433000      2017-01-06\n",
       "1           Rockstar         1831000      2017-09-15\n",
       "2          One Dance         1817000      2016-04-05\n",
       "3             Closer         1724000      2016-07-29\n",
       "4  Thinking Out Loud         1493000      2014-06-20"
      ]
     },
     "execution_count": 5,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "formato_data = \"%d/%m/%Y\"\n",
    "spotify_mais_escutadas = {\"música\": [\"Shape of You\", \"Rockstar\", \"One Dance\", \"Closer\", \"Thinking Out Loud\"],\n",
    "                          \"número_streams\": [2433000, 1831000, 1817000, 1724000, 1493000],\n",
    "                          \"data_publicação\": [pd.to_datetime(\"6/1/2017\", format=formato_data),\n",
    "                                              pd.to_datetime(\"15/9/2017\", format=formato_data),\n",
    "                                              pd.to_datetime(\"5/4/2016\", format=formato_data),\n",
    "                                              pd.to_datetime(\"29/7/2016\", format=formato_data),\n",
    "                                              pd.to_datetime(\"20/6/2014\", format=formato_data)]}\n",
    "spotify_mais_escutadas_df = pd.DataFrame(spotify_mais_escutadas)\n",
    "spotify_mais_escutadas_df"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {
    "colab_type": "text",
    "id": "4W94rSHu5qkK"
   },
   "source": [
    "Nesse workshop trabalharemos majoritariamente com `DataFrame`s. Apesar de não ser uma solução universal para todos os problemas, o conjunto de métodos e ferramentas que acompanham o tipo `DataFrame` provêm uma base sólida e de uso simples para a maioria dos casos de uso."
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {
    "colab_type": "text",
    "id": "MwdLQkiu9exO"
   },
   "source": [
    "### CSV\n",
    "\n",
    "Para criar um `DataFrame` a partir de um arquivo `.csv` usamos a função [`read_csv`](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.read_csv.html). Arquivos `.csv` têm por definição vírgulas como separadores de campos. Contudo, podemos mudar o argumento do parâmetro `sep` da função para criar um `DataFrame` a partir de arquivos delimitados por outros caracteres, como por exemplo o *tab*."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 6,
   "metadata": {
    "colab": {},
    "colab_type": "code",
    "id": "fgUvRKPg2vuC"
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   "outputs": [
    {
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       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>Func.refGene</th>\n",
       "      <th>Gene.refGene</th>\n",
       "      <th>GeneDetail.refGene</th>\n",
       "      <th>ExonicFunc.refGene</th>\n",
       "      <th>AAChange.refGene</th>\n",
       "      <th>...</th>\n",
       "      <th>VCF_CHR</th>\n",
       "      <th>VCF_POS</th>\n",
       "      <th>VCF_ID</th>\n",
       "      <th>VCF_REF</th>\n",
       "      <th>VCF_ALT</th>\n",
       "      <th>VCF_QUAL_2</th>\n",
       "      <th>VCF_FILTER</th>\n",
       "      <th>VCF_INFO</th>\n",
       "      <th>VCF_FORMAT</th>\n",
       "      <th>VCF_SAMPLE_NAME</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>89622938</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>NM_001126049:c.-694T&gt;G</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>290.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1266,552:1820:99:319,0,12285</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>89623142</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>NM_001126049:c.-898G&gt;A</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>7963.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:306,267:574:99:7992,0,9864</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623861</td>\n",
       "      <td>89623861</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>splicing</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304717:exon1:c.154+1T&gt;-;NM_001304717:exo...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623860</td>\n",
       "      <td>rs71022512</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>234.80</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,6:7:18:272,18,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>89623901</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>exonic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>nonsynonymous SNV</td>\n",
       "      <td>PTEN:NM_001304717:exon2:c.194G&gt;C:p.C65S</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>rs2943772</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>440.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,14:14:36:469,36,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>89654121</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>rs139651072</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>216.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:10,8:18:99:245,0,289</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>5 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "     Chr     Start       End Ref Alt Func.refGene Gene.refGene  \\\n",
       "0  chr10  89622938  89622938   A   C         UTR5         KLLN   \n",
       "1  chr10  89623142  89623142   C   T         UTR5         KLLN   \n",
       "2  chr10  89623861  89623861   T   -     splicing         PTEN   \n",
       "3  chr10  89623901  89623901   G   C       exonic         PTEN   \n",
       "4  chr10  89654121  89654121   T   C     intronic         PTEN   \n",
       "\n",
       "                                  GeneDetail.refGene ExonicFunc.refGene  \\\n",
       "0                             NM_001126049:c.-694T>G                  .   \n",
       "1                             NM_001126049:c.-898G>A                  .   \n",
       "2  NM_001304717:exon1:c.154+1T>-;NM_001304717:exo...                  .   \n",
       "3                                                  .  nonsynonymous SNV   \n",
       "4                                                  .                  .   \n",
       "\n",
       "                          AAChange.refGene  ... VCF_CHR   VCF_POS  \\\n",
       "0                                        .  ...   chr10  89622938   \n",
       "1                                        .  ...   chr10  89623142   \n",
       "2                                        .  ...   chr10  89623860   \n",
       "3  PTEN:NM_001304717:exon2:c.194G>C:p.C65S  ...   chr10  89623901   \n",
       "4                                        .  ...   chr10  89654121   \n",
       "\n",
       "        VCF_ID VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "0            .       A       C     290.77          .   \n",
       "1            .       C       T    7963.77          .   \n",
       "2   rs71022512      CT       C     234.80          .   \n",
       "3    rs2943772       G       C     440.77          .   \n",
       "4  rs139651072       T       C     216.77          .   \n",
       "\n",
       "                                            VCF_INFO      VCF_FORMAT  \\\n",
       "0  ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....  GT:AD:DP:GQ:PL   \n",
       "1  ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...  GT:AD:DP:GQ:PL   \n",
       "2  AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...  GT:AD:DP:GQ:PL   \n",
       "3  ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...  GT:AD:DP:GQ:PL   \n",
       "4  ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...  GT:AD:DP:GQ:PL   \n",
       "\n",
       "                    VCF_SAMPLE_NAME  \n",
       "0  0/1:1266,552:1820:99:319,0,12285  \n",
       "1    0/1:306,267:574:99:7992,0,9864  \n",
       "2             1/1:0,6:7:18:272,18,0  \n",
       "3           1/1:0,14:14:36:469,36,0  \n",
       "4          0/1:10,8:18:99:245,0,289  \n",
       "\n",
       "[5 rows x 183 columns]"
      ]
     },
     "execution_count": 6,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df = pd.read_csv(\"https://www.dropbox.com/s/xsxbyel2t9fjidl/GNMK_WORKSHOP-001.avinput.hg19_multianno.ann.txt?dl=1\", sep=\"\\t\")\n",
    "multianno_df.head()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {
    "colab_type": "text",
    "id": "vQRqH4UOCq1c"
   },
   "source": [
    "O cabeçalho do `DataFrame` é por padrão inferido pela função `read_csv` com base na primeira linha do arquivo. Esse comportamento pode ser modificado usando uma combinação de valores para os parâmetros `header` e `names`."
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {
    "colab_type": "text",
    "id": "3mt6qXVyFa0-"
   },
   "source": [
    "### Excel\n",
    "\n",
    "Para criar um `DataFrame` a partir de uma planilha do Excel usamos a função [`read_excel`](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.read_excel.html). Essa função suporta arquivos com extensão *xls*, *xlsx*, *xlsm*, *xlsb*, e *odf*. Olhando por uma perspectiva de caixa preta, o comportamento da função `read_excel` é bem similar ao comportamento da função `read_csv`: Ambas recebem um arquivo e criam um `DataFrame` a partir do conteúdo desses arquivos."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 7,
   "metadata": {
    "colab": {},
    "colab_type": "code",
    "id": "wiRk5VxzM26N"
   },
   "outputs": [
    {
     "data": {
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       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
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       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>RANK</th>\n",
       "      <th>CLINVAR</th>\n",
       "      <th>CLINVAR_COMMENTS</th>\n",
       "      <th>OMIM</th>\n",
       "      <th>OMIM_COMMENTS</th>\n",
       "      <th>UNIPROT</th>\n",
       "      <th>UNIPROT_COMMENTS</th>\n",
       "      <th>Func</th>\n",
       "      <th>Gene</th>\n",
       "      <th>ExonicFunc</th>\n",
       "      <th>...</th>\n",
       "      <th>MutationTaster_pred</th>\n",
       "      <th>GERP++_RS</th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>REPEATMASK</th>\n",
       "      <th>InterVar(automated)</th>\n",
       "      <th>InterVar_Rules</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>29</td>\n",
       "      <td>OK</td>\n",
       "      <td>Pathogenic</td>\n",
       "      <td>OK</td>\n",
       "      <td>Name={Breast cancer, male, susceptibility to},...</td>\n",
       "      <td>OK</td>\n",
       "      <td>SwissProt: P51587 # Breast cancer (BC) [MIM:11...</td>\n",
       "      <td>exonic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>frameshift deletion</td>\n",
       "      <td>...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32929058</td>\n",
       "      <td>32929059</td>\n",
       "      <td>TC</td>\n",
       "      <td>-</td>\n",
       "      <td>NaN</td>\n",
       "      <td>.</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>11</td>\n",
       "      <td>OK</td>\n",
       "      <td>Benign</td>\n",
       "      <td>OK</td>\n",
       "      <td>Name=Bannayan-Riley-Ruvalcaba syndrome, 153480...</td>\n",
       "      <td>OK</td>\n",
       "      <td>SwissProt: P60484 # Cowden syndrome 1 (CWS1) [...</td>\n",
       "      <td>splicing</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623861</td>\n",
       "      <td>89623861</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>NaN</td>\n",
       "      <td>.</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>11</td>\n",
       "      <td>OK</td>\n",
       "      <td>Benign</td>\n",
       "      <td>OK</td>\n",
       "      <td>Name=Blepharocheilodontic syndrome 1, 119580 (...</td>\n",
       "      <td>OK</td>\n",
       "      <td>SwissProt: P12830 # Hereditary diffuse gastric...</td>\n",
       "      <td>splicing</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>chr16</td>\n",
       "      <td>68771372</td>\n",
       "      <td>68771372</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>NaN</td>\n",
       "      <td>.</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>10</td>\n",
       "      <td>OK</td>\n",
       "      <td>Benign</td>\n",
       "      <td>OK</td>\n",
       "      <td>Name=Bannayan-Riley-Ruvalcaba syndrome, 153480...</td>\n",
       "      <td>OK</td>\n",
       "      <td>SwissProt: P60484 # Cowden syndrome 1 (CWS1) [...</td>\n",
       "      <td>exonic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>nonsynonymous SNV</td>\n",
       "      <td>...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>89623901</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>Low_complexity_Low_complexity_GC_rich</td>\n",
       "      <td>Benign</td>\n",
       "      <td>BA1, BS1</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>10</td>\n",
       "      <td>OK</td>\n",
       "      <td>Benign</td>\n",
       "      <td>OK</td>\n",
       "      <td>Name={Breast cancer, male, susceptibility to},...</td>\n",
       "      <td>OK</td>\n",
       "      <td>SwissProt: P51587 # Breast cancer (BC) [MIM:11...</td>\n",
       "      <td>exonic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>nonsynonymous SNV</td>\n",
       "      <td>...</td>\n",
       "      <td>P</td>\n",
       "      <td>5.07</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32929387</td>\n",
       "      <td>32929387</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>NaN</td>\n",
       "      <td>Benign</td>\n",
       "      <td>BA1, BS1, BS2, BP1, BP6</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>5 rows × 45 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "   RANK CLINVAR CLINVAR_COMMENTS OMIM  \\\n",
       "0    29      OK       Pathogenic   OK   \n",
       "1    11      OK           Benign   OK   \n",
       "2    11      OK           Benign   OK   \n",
       "3    10      OK           Benign   OK   \n",
       "4    10      OK           Benign   OK   \n",
       "\n",
       "                                       OMIM_COMMENTS UNIPROT  \\\n",
       "0  Name={Breast cancer, male, susceptibility to},...      OK   \n",
       "1  Name=Bannayan-Riley-Ruvalcaba syndrome, 153480...      OK   \n",
       "2  Name=Blepharocheilodontic syndrome 1, 119580 (...      OK   \n",
       "3  Name=Bannayan-Riley-Ruvalcaba syndrome, 153480...      OK   \n",
       "4  Name={Breast cancer, male, susceptibility to},...      OK   \n",
       "\n",
       "                                    UNIPROT_COMMENTS      Func   Gene  \\\n",
       "0  SwissProt: P51587 # Breast cancer (BC) [MIM:11...    exonic  BRCA2   \n",
       "1  SwissProt: P60484 # Cowden syndrome 1 (CWS1) [...  splicing   PTEN   \n",
       "2  SwissProt: P12830 # Hereditary diffuse gastric...  splicing   CDH1   \n",
       "3  SwissProt: P60484 # Cowden syndrome 1 (CWS1) [...    exonic   PTEN   \n",
       "4  SwissProt: P51587 # Breast cancer (BC) [MIM:11...    exonic  BRCA2   \n",
       "\n",
       "            ExonicFunc  ... MutationTaster_pred GERP++_RS    Chr     Start  \\\n",
       "0  frameshift deletion  ...                   .         .  chr13  32929058   \n",
       "1                    .  ...                   .         .  chr10  89623861   \n",
       "2                    .  ...                   .         .  chr16  68771372   \n",
       "3    nonsynonymous SNV  ...                   .         .  chr10  89623901   \n",
       "4    nonsynonymous SNV  ...                   P      5.07  chr13  32929387   \n",
       "\n",
       "        End Ref Alt                             REPEATMASK  \\\n",
       "0  32929059  TC   -                                    NaN   \n",
       "1  89623861   T   -                                    NaN   \n",
       "2  68771372   C   T                                    NaN   \n",
       "3  89623901   G   C  Low_complexity_Low_complexity_GC_rich   \n",
       "4  32929387   T   C                                    NaN   \n",
       "\n",
       "   InterVar(automated)           InterVar_Rules  \n",
       "0                    .                      NaN  \n",
       "1                    .                      NaN  \n",
       "2                    .                      NaN  \n",
       "3               Benign                 BA1, BS1  \n",
       "4               Benign  BA1, BS1, BS2, BP1, BP6  \n",
       "\n",
       "[5 rows x 45 columns]"
      ]
     },
     "execution_count": 7,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "aba_exonic_df = pd.read_excel(\"https://www.dropbox.com/s/gyx0hqco6dhtwg2/GNMK_WORKSHOP-001.xlsx?dl=1\")\n",
    "aba_exonic_df.head()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {
    "colab_type": "text",
    "id": "VNyUozTfNaZH"
   },
   "source": [
    "Caso a planilha usada como parâmetro contenha mais de uma aba, a função `read_excel` irá por padrão criar o `DataFrame` a partir dos dados contidos na primeira aba da planilha. Para usar dados de outras abas podemos mudar o argumento do parâmetro `sheet_name`."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 8,
   "metadata": {
    "colab": {},
    "colab_type": "code",
    "id": "UswN7sqVn3Xs"
   },
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
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       "\n",
       "    .dataframe tbody tr th {\n",
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       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>RANK</th>\n",
       "      <th>CLINVAR</th>\n",
       "      <th>CLINVAR_COMMENTS</th>\n",
       "      <th>OMIM</th>\n",
       "      <th>OMIM_COMMENTS</th>\n",
       "      <th>UNIPROT</th>\n",
       "      <th>UNIPROT_COMMENTS</th>\n",
       "      <th>Func</th>\n",
       "      <th>Gene</th>\n",
       "      <th>ExonicFunc</th>\n",
       "      <th>...</th>\n",
       "      <th>MutationTaster_pred</th>\n",
       "      <th>GERP++_RS</th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>REPEATMASK</th>\n",
       "      <th>InterVar(automated)</th>\n",
       "      <th>InterVar_Rules</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>17</td>\n",
       "      <td>NOK</td>\n",
       "      <td>.</td>\n",
       "      <td>OK</td>\n",
       "      <td>Name=Cowden syndrome 4, 615107 (3)</td>\n",
       "      <td>OK</td>\n",
       "      <td>SwissProt: B2CW77 # Cowden syndrome 4 (CWS4) [...</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>89622938</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>NaN</td>\n",
       "      <td>.</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>17</td>\n",
       "      <td>OK</td>\n",
       "      <td>Conflicting_interpretations_of_pathogenicity</td>\n",
       "      <td>OK</td>\n",
       "      <td>Name=Cowden syndrome 4, 615107 (3)</td>\n",
       "      <td>OK</td>\n",
       "      <td>SwissProt: B2CW77 # Cowden syndrome 4 (CWS4) [...</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>89623142</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>NaN</td>\n",
       "      <td>.</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>17</td>\n",
       "      <td>NOK</td>\n",
       "      <td>.</td>\n",
       "      <td>OK</td>\n",
       "      <td>Name={Breast cancer, susceptibility to}, 11448...</td>\n",
       "      <td>OK</td>\n",
       "      <td>SwissProt: Q86YC2 # Breast cancer (BC) [MIM:11...</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PALB2</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>chr16</td>\n",
       "      <td>23640160</td>\n",
       "      <td>23640160</td>\n",
       "      <td>G</td>\n",
       "      <td>T</td>\n",
       "      <td>SINE_Alu_AluSx1</td>\n",
       "      <td>.</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>17</td>\n",
       "      <td>NOK</td>\n",
       "      <td>.</td>\n",
       "      <td>OK</td>\n",
       "      <td>Name=Blepharocheilodontic syndrome 1, 119580 (...</td>\n",
       "      <td>OK</td>\n",
       "      <td>SwissProt: P12830 # Hereditary diffuse gastric...</td>\n",
       "      <td>intronic</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>chr16</td>\n",
       "      <td>68820717</td>\n",
       "      <td>68820717</td>\n",
       "      <td>C</td>\n",
       "      <td>A</td>\n",
       "      <td>NaN</td>\n",
       "      <td>.</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>17</td>\n",
       "      <td>NOK</td>\n",
       "      <td>.</td>\n",
       "      <td>OK</td>\n",
       "      <td>Name=Blepharocheilodontic syndrome 1, 119580 (...</td>\n",
       "      <td>OK</td>\n",
       "      <td>SwissProt: P12830 # Hereditary diffuse gastric...</td>\n",
       "      <td>intronic</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>chr16</td>\n",
       "      <td>68856474</td>\n",
       "      <td>68856474</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>NaN</td>\n",
       "      <td>.</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>5 rows × 45 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "   RANK CLINVAR                              CLINVAR_COMMENTS OMIM  \\\n",
       "0    17     NOK                                             .   OK   \n",
       "1    17      OK  Conflicting_interpretations_of_pathogenicity   OK   \n",
       "2    17     NOK                                             .   OK   \n",
       "3    17     NOK                                             .   OK   \n",
       "4    17     NOK                                             .   OK   \n",
       "\n",
       "                                       OMIM_COMMENTS UNIPROT  \\\n",
       "0                 Name=Cowden syndrome 4, 615107 (3)      OK   \n",
       "1                 Name=Cowden syndrome 4, 615107 (3)      OK   \n",
       "2  Name={Breast cancer, susceptibility to}, 11448...      OK   \n",
       "3  Name=Blepharocheilodontic syndrome 1, 119580 (...      OK   \n",
       "4  Name=Blepharocheilodontic syndrome 1, 119580 (...      OK   \n",
       "\n",
       "                                    UNIPROT_COMMENTS      Func   Gene  \\\n",
       "0  SwissProt: B2CW77 # Cowden syndrome 4 (CWS4) [...      UTR5   KLLN   \n",
       "1  SwissProt: B2CW77 # Cowden syndrome 4 (CWS4) [...      UTR5   KLLN   \n",
       "2  SwissProt: Q86YC2 # Breast cancer (BC) [MIM:11...  intronic  PALB2   \n",
       "3  SwissProt: P12830 # Hereditary diffuse gastric...  intronic   CDH1   \n",
       "4  SwissProt: P12830 # Hereditary diffuse gastric...  intronic   CDH1   \n",
       "\n",
       "  ExonicFunc  ... MutationTaster_pred GERP++_RS    Chr     Start       End  \\\n",
       "0          .  ...                   .         .  chr10  89622938  89622938   \n",
       "1          .  ...                   .         .  chr10  89623142  89623142   \n",
       "2          .  ...                   .         .  chr16  23640160  23640160   \n",
       "3          .  ...                   .         .  chr16  68820717  68820717   \n",
       "4          .  ...                   .         .  chr16  68856474  68856474   \n",
       "\n",
       "  Ref Alt       REPEATMASK  InterVar(automated)  InterVar_Rules  \n",
       "0   A   C              NaN                    .             NaN  \n",
       "1   C   T              NaN                    .             NaN  \n",
       "2   G   T  SINE_Alu_AluSx1                    .             NaN  \n",
       "3   C   A              NaN                    .             NaN  \n",
       "4   T   C              NaN                    .             NaN  \n",
       "\n",
       "[5 rows x 45 columns]"
      ]
     },
     "execution_count": 8,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# O parâmetro sheet_name pode receber uma string com o nome da aba cujo dados serão carregados\n",
    "aba_intronic_df = pd.read_excel(\"https://www.dropbox.com/s/gyx0hqco6dhtwg2/GNMK_WORKSHOP-001.xlsx?dl=1\", sheet_name=\"INTRONIC\")\n",
    "aba_intronic_df.head()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 9,
   "metadata": {
    "colab": {},
    "colab_type": "code",
    "id": "-hEXXQxgo6tf"
   },
   "outputs": [
    {
     "data": {
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       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>RANK</th>\n",
       "      <th>CLINVAR</th>\n",
       "      <th>CLINVAR_COMMENTS</th>\n",
       "      <th>OMIM</th>\n",
       "      <th>OMIM_COMMENTS</th>\n",
       "      <th>UNIPROT</th>\n",
       "      <th>UNIPROT_COMMENTS</th>\n",
       "      <th>Func</th>\n",
       "      <th>Gene</th>\n",
       "      <th>ExonicFunc</th>\n",
       "      <th>...</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>REPEATMASK</th>\n",
       "      <th>InterVar(automated)</th>\n",
       "      <th>InterVar_Rules</th>\n",
       "      <th>QUAL.1</th>\n",
       "      <th>FILTER.1</th>\n",
       "      <th>INFO</th>\n",
       "      <th>FORMAT</th>\n",
       "      <th>FORMAT_INFO</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>29</td>\n",
       "      <td>OK</td>\n",
       "      <td>Pathogenic</td>\n",
       "      <td>OK</td>\n",
       "      <td>Name={Breast cancer, male, susceptibility to},...</td>\n",
       "      <td>OK</td>\n",
       "      <td>SwissProt: P51587 # Breast cancer (BC) [MIM:11...</td>\n",
       "      <td>exonic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>frameshift deletion</td>\n",
       "      <td>...</td>\n",
       "      <td>TC</td>\n",
       "      <td>-</td>\n",
       "      <td>NaN</td>\n",
       "      <td>.</td>\n",
       "      <td>NaN</td>\n",
       "      <td>33599.70</td>\n",
       "      <td>PASS</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=2.352;DB;DP=1613...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:853,734:1613:99:33637,0,39748</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>17</td>\n",
       "      <td>NOK</td>\n",
       "      <td>.</td>\n",
       "      <td>OK</td>\n",
       "      <td>Name=Cowden syndrome 4, 615107 (3)</td>\n",
       "      <td>OK</td>\n",
       "      <td>SwissProt: B2CW77 # Cowden syndrome 4 (CWS4) [...</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>NaN</td>\n",
       "      <td>.</td>\n",
       "      <td>NaN</td>\n",
       "      <td>290.77</td>\n",
       "      <td>PASS</td>\n",
       "      <td>ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1266,552:1820:99:319,0,12285</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>17</td>\n",
       "      <td>OK</td>\n",
       "      <td>Conflicting_interpretations_of_pathogenicity</td>\n",
       "      <td>OK</td>\n",
       "      <td>Name=Cowden syndrome 4, 615107 (3)</td>\n",
       "      <td>OK</td>\n",
       "      <td>SwissProt: B2CW77 # Cowden syndrome 4 (CWS4) [...</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>NaN</td>\n",
       "      <td>.</td>\n",
       "      <td>NaN</td>\n",
       "      <td>7963.77</td>\n",
       "      <td>PASS</td>\n",
       "      <td>ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:306,267:574:99:7992,0,9864</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>17</td>\n",
       "      <td>NOK</td>\n",
       "      <td>.</td>\n",
       "      <td>OK</td>\n",
       "      <td>Name={Breast cancer, susceptibility to}, 11448...</td>\n",
       "      <td>OK</td>\n",
       "      <td>SwissProt: Q86YC2 # Breast cancer (BC) [MIM:11...</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PALB2</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>G</td>\n",
       "      <td>T</td>\n",
       "      <td>SINE_Alu_AluSx1</td>\n",
       "      <td>.</td>\n",
       "      <td>NaN</td>\n",
       "      <td>35.77</td>\n",
       "      <td>PASS</td>\n",
       "      <td>ABHet=0.6;AC=1;AF=0.5;AN=2;BaseQRankSum=0;DP=5...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:3,2:5:64:64,0,103</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>17</td>\n",
       "      <td>NOK</td>\n",
       "      <td>.</td>\n",
       "      <td>OK</td>\n",
       "      <td>Name=Blepharocheilodontic syndrome 1, 119580 (...</td>\n",
       "      <td>OK</td>\n",
       "      <td>SwissProt: P12830 # Hereditary diffuse gastric...</td>\n",
       "      <td>intronic</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>C</td>\n",
       "      <td>A</td>\n",
       "      <td>NaN</td>\n",
       "      <td>.</td>\n",
       "      <td>NaN</td>\n",
       "      <td>680.77</td>\n",
       "      <td>PASS</td>\n",
       "      <td>ABHet=0.587;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.4...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:37,26:63:99:709,0,1225</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>5 rows × 50 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "   RANK CLINVAR                              CLINVAR_COMMENTS OMIM  \\\n",
       "0    29      OK                                    Pathogenic   OK   \n",
       "1    17     NOK                                             .   OK   \n",
       "2    17      OK  Conflicting_interpretations_of_pathogenicity   OK   \n",
       "3    17     NOK                                             .   OK   \n",
       "4    17     NOK                                             .   OK   \n",
       "\n",
       "                                       OMIM_COMMENTS UNIPROT  \\\n",
       "0  Name={Breast cancer, male, susceptibility to},...      OK   \n",
       "1                 Name=Cowden syndrome 4, 615107 (3)      OK   \n",
       "2                 Name=Cowden syndrome 4, 615107 (3)      OK   \n",
       "3  Name={Breast cancer, susceptibility to}, 11448...      OK   \n",
       "4  Name=Blepharocheilodontic syndrome 1, 119580 (...      OK   \n",
       "\n",
       "                                    UNIPROT_COMMENTS      Func   Gene  \\\n",
       "0  SwissProt: P51587 # Breast cancer (BC) [MIM:11...    exonic  BRCA2   \n",
       "1  SwissProt: B2CW77 # Cowden syndrome 4 (CWS4) [...      UTR5   KLLN   \n",
       "2  SwissProt: B2CW77 # Cowden syndrome 4 (CWS4) [...      UTR5   KLLN   \n",
       "3  SwissProt: Q86YC2 # Breast cancer (BC) [MIM:11...  intronic  PALB2   \n",
       "4  SwissProt: P12830 # Hereditary diffuse gastric...  intronic   CDH1   \n",
       "\n",
       "            ExonicFunc  ... Ref Alt       REPEATMASK  InterVar(automated)  \\\n",
       "0  frameshift deletion  ...  TC   -              NaN                    .   \n",
       "1                    .  ...   A   C              NaN                    .   \n",
       "2                    .  ...   C   T              NaN                    .   \n",
       "3                    .  ...   G   T  SINE_Alu_AluSx1                    .   \n",
       "4                    .  ...   C   A              NaN                    .   \n",
       "\n",
       "   InterVar_Rules    QUAL.1 FILTER.1  \\\n",
       "0             NaN  33599.70     PASS   \n",
       "1             NaN    290.77     PASS   \n",
       "2             NaN   7963.77     PASS   \n",
       "3             NaN     35.77     PASS   \n",
       "4             NaN    680.77     PASS   \n",
       "\n",
       "                                                INFO          FORMAT  \\\n",
       "0  AC=1;AF=0.5;AN=2;BaseQRankSum=2.352;DB;DP=1613...  GT:AD:DP:GQ:PL   \n",
       "1  ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....  GT:AD:DP:GQ:PL   \n",
       "2  ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...  GT:AD:DP:GQ:PL   \n",
       "3  ABHet=0.6;AC=1;AF=0.5;AN=2;BaseQRankSum=0;DP=5...  GT:AD:DP:GQ:PL   \n",
       "4  ABHet=0.587;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.4...  GT:AD:DP:GQ:PL   \n",
       "\n",
       "                         FORMAT_INFO  \n",
       "0  0/1:853,734:1613:99:33637,0,39748  \n",
       "1   0/1:1266,552:1820:99:319,0,12285  \n",
       "2     0/1:306,267:574:99:7992,0,9864  \n",
       "3              0/1:3,2:5:64:64,0,103  \n",
       "4         0/1:37,26:63:99:709,0,1225  \n",
       "\n",
       "[5 rows x 50 columns]"
      ]
     },
     "execution_count": 9,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Também podemos usar inteiros como parâmetro, fazendo referência a ordem das abas (index zero)\n",
    "aba_raw_df = pd.read_excel(\"https://www.dropbox.com/s/gyx0hqco6dhtwg2/GNMK_WORKSHOP-001.xlsx?dl=1\", sheet_name=3)\n",
    "aba_raw_df.head()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 10,
   "metadata": {
    "colab": {},
    "colab_type": "code",
    "id": "qRkFU3mMppBm"
   },
   "outputs": [
    {
     "data": {
      "text/plain": [
       "dict"
      ]
     },
     "execution_count": 10,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Se quisermos usar dados de todas as abas da planilha passamos None para o sheet_name\n",
    "planilha_completa = pd.read_excel(\"https://www.dropbox.com/s/gyx0hqco6dhtwg2/GNMK_WORKSHOP-001.xlsx?dl=1\", sheet_name=None)\n",
    "type(planilha_completa)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 11,
   "metadata": {
    "colab": {},
    "colab_type": "code",
    "id": "EJAv9JTFtNUX"
   },
   "outputs": [],
   "source": [
    "# E então podemos acessar os dados de todas as abas a partir de um único objeto\n",
    "aba_exonic_df = planilha_completa[\"EXONIC\"]\n",
    "aba_exonic_mosaic_df = planilha_completa[\"EXONIC MOSAIC\"]\n",
    "aba_intronic_df = planilha_completa[\"INTRONIC\"]\n",
    "aba_raw_df = planilha_completa[\"RAW\"]"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### Python dicionários\n",
    "\n",
    "Quando introduzimos `DataFrame`s usamos como exemplo um dicionário python. Tendo um dicionário basta usar a função `DataFrame()` para que ele seja transformado."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 12,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>dia</th>\n",
       "      <th>temperatura</th>\n",
       "      <th>velocidade_vento</th>\n",
       "      <th>evento</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>1/1/2017</td>\n",
       "      <td>32</td>\n",
       "      <td>6</td>\n",
       "      <td>Chuva</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>1/2/2017</td>\n",
       "      <td>35</td>\n",
       "      <td>7</td>\n",
       "      <td>Sol</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>1/3/2017</td>\n",
       "      <td>28</td>\n",
       "      <td>2</td>\n",
       "      <td>Neve</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>1/4/2017</td>\n",
       "      <td>24</td>\n",
       "      <td>7</td>\n",
       "      <td>Neve</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>1/5/2017</td>\n",
       "      <td>32</td>\n",
       "      <td>4</td>\n",
       "      <td>Chuva</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>5</th>\n",
       "      <td>1/6/2017</td>\n",
       "      <td>31</td>\n",
       "      <td>2</td>\n",
       "      <td>Sol</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "</div>"
      ],
      "text/plain": [
       "        dia  temperatura  velocidade_vento evento\n",
       "0  1/1/2017           32                 6  Chuva\n",
       "1  1/2/2017           35                 7    Sol\n",
       "2  1/3/2017           28                 2   Neve\n",
       "3  1/4/2017           24                 7   Neve\n",
       "4  1/5/2017           32                 4  Chuva\n",
       "5  1/6/2017           31                 2    Sol"
      ]
     },
     "execution_count": 12,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "climas_datas = {\n",
    "    'dia': ['1/1/2017', '1/2/2017', '1/3/2017', '1/4/2017', '1/5/2017', '1/6/2017'],\n",
    "    'temperatura': [32, 35, 28, 24, 32, 31],\n",
    "    'velocidade_vento': [6, 7, 2, 7, 4, 2],\n",
    "    'evento': ['Chuva', 'Sol', 'Neve', 'Neve', 'Chuva', 'Sol']\n",
    "}\n",
    "df = pd.DataFrame(climas_datas)\n",
    "df"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### Python lista de tuplas\n",
    "\n",
    "É possível criar um `DataFrame` a partir de uma lista de tuplas, sendo necessário especificar o nome das colunas pelo argumento `columns`, por exemplo:"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 13,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>dia</th>\n",
       "      <th>temperatura</th>\n",
       "      <th>velocidade_vento</th>\n",
       "      <th>evento</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>1/1/2017</td>\n",
       "      <td>32</td>\n",
       "      <td>6</td>\n",
       "      <td>Chuva</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>1/2/2017</td>\n",
       "      <td>35</td>\n",
       "      <td>7</td>\n",
       "      <td>Sol</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>1/3/2017</td>\n",
       "      <td>28</td>\n",
       "      <td>2</td>\n",
       "      <td>Neve</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "</div>"
      ],
      "text/plain": [
       "        dia  temperatura  velocidade_vento evento\n",
       "0  1/1/2017           32                 6  Chuva\n",
       "1  1/2/2017           35                 7    Sol\n",
       "2  1/3/2017           28                 2   Neve"
      ]
     },
     "execution_count": 13,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "climas_datas = [\n",
    "    ('1/1/2017', 32, 6, 'Chuva'),\n",
    "    ('1/2/2017', 35, 7, 'Sol'),\n",
    "    ('1/3/2017', 28, 2, 'Neve')\n",
    "]\n",
    "\n",
    "df = pd.DataFrame(climas_datas, columns=[\n",
    "    'dia', 'temperatura', 'velocidade_vento', 'evento'\n",
    "])\n",
    "df"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### Python lista de dicionários\n",
    "\n",
    "É possível criar um DataFrame a partir de uma lista de dicionários, como:"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 14,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>dia</th>\n",
       "      <th>temperatura</th>\n",
       "      <th>velocidade_vento</th>\n",
       "      <th>evento</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>1/1/2017</td>\n",
       "      <td>32</td>\n",
       "      <td>6</td>\n",
       "      <td>Chuva</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>1/2/2017</td>\n",
       "      <td>35</td>\n",
       "      <td>7</td>\n",
       "      <td>Sunny</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>1/3/2017</td>\n",
       "      <td>28</td>\n",
       "      <td>2</td>\n",
       "      <td>Neve</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "</div>"
      ],
      "text/plain": [
       "        dia  temperatura  velocidade_vento evento\n",
       "0  1/1/2017           32                 6  Chuva\n",
       "1  1/2/2017           35                 7  Sunny\n",
       "2  1/3/2017           28                 2   Neve"
      ]
     },
     "execution_count": 14,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "climas_datas = [\n",
    "    {'dia': '1/1/2017', 'temperatura': 32, 'velocidade_vento': 6,'evento': 'Chuva'},\n",
    "    {'dia': '1/2/2017', 'temperatura': 35, 'velocidade_vento': 7,'evento': 'Sunny'},\n",
    "    {'dia': '1/3/2017', 'temperatura': 28, 'velocidade_vento': 2,'evento': 'Neve'} \n",
    "]\n",
    "df = pd.DataFrame(climas_datas)\n",
    "df"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Existem vários outros tipos de arquivos que podem ser abertos com Pandas, criando `DataFrame`s, alguns exemplos são `JSON`, `HTML`, `HDF5`, `SQL`, `Parquet` e outros, para mais formatos segue a lista de [*IO tools*](https://pandas.pydata.org/pandas-docs/stable/user_guide/io.html) possíveis."
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### Manipulação básica de `DataFrame`s"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "Para saber o tamanho do `DataFrame` podemos usar o método `shape` que retorna uma tupla com os valores `(linhas, colunas)` "
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 15,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "(92, 183)"
      ]
     },
     "execution_count": 15,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df.shape"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "Visualizando as primeiras e as últimas linhas dos dados."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 16,
   "metadata": {},
   "outputs": [
    {
     "data": {
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       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
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       "\n",
       "    .dataframe tbody tr th {\n",
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       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>Func.refGene</th>\n",
       "      <th>Gene.refGene</th>\n",
       "      <th>GeneDetail.refGene</th>\n",
       "      <th>ExonicFunc.refGene</th>\n",
       "      <th>AAChange.refGene</th>\n",
       "      <th>...</th>\n",
       "      <th>VCF_CHR</th>\n",
       "      <th>VCF_POS</th>\n",
       "      <th>VCF_ID</th>\n",
       "      <th>VCF_REF</th>\n",
       "      <th>VCF_ALT</th>\n",
       "      <th>VCF_QUAL_2</th>\n",
       "      <th>VCF_FILTER</th>\n",
       "      <th>VCF_INFO</th>\n",
       "      <th>VCF_FORMAT</th>\n",
       "      <th>VCF_SAMPLE_NAME</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>89622938</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>NM_001126049:c.-694T&gt;G</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>290.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1266,552:1820:99:319,0,12285</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>89623142</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>NM_001126049:c.-898G&gt;A</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>7963.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:306,267:574:99:7992,0,9864</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623861</td>\n",
       "      <td>89623861</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>splicing</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304717:exon1:c.154+1T&gt;-;NM_001304717:exo...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623860</td>\n",
       "      <td>rs71022512</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>234.80</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,6:7:18:272,18,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>89623901</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>exonic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>nonsynonymous SNV</td>\n",
       "      <td>PTEN:NM_001304717:exon2:c.194G&gt;C:p.C65S</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>rs2943772</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>440.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,14:14:36:469,36,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>89654121</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>rs139651072</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>216.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:10,8:18:99:245,0,289</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>5 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "     Chr     Start       End Ref Alt Func.refGene Gene.refGene  \\\n",
       "0  chr10  89622938  89622938   A   C         UTR5         KLLN   \n",
       "1  chr10  89623142  89623142   C   T         UTR5         KLLN   \n",
       "2  chr10  89623861  89623861   T   -     splicing         PTEN   \n",
       "3  chr10  89623901  89623901   G   C       exonic         PTEN   \n",
       "4  chr10  89654121  89654121   T   C     intronic         PTEN   \n",
       "\n",
       "                                  GeneDetail.refGene ExonicFunc.refGene  \\\n",
       "0                             NM_001126049:c.-694T>G                  .   \n",
       "1                             NM_001126049:c.-898G>A                  .   \n",
       "2  NM_001304717:exon1:c.154+1T>-;NM_001304717:exo...                  .   \n",
       "3                                                  .  nonsynonymous SNV   \n",
       "4                                                  .                  .   \n",
       "\n",
       "                          AAChange.refGene  ... VCF_CHR   VCF_POS  \\\n",
       "0                                        .  ...   chr10  89622938   \n",
       "1                                        .  ...   chr10  89623142   \n",
       "2                                        .  ...   chr10  89623860   \n",
       "3  PTEN:NM_001304717:exon2:c.194G>C:p.C65S  ...   chr10  89623901   \n",
       "4                                        .  ...   chr10  89654121   \n",
       "\n",
       "        VCF_ID VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "0            .       A       C     290.77          .   \n",
       "1            .       C       T    7963.77          .   \n",
       "2   rs71022512      CT       C     234.80          .   \n",
       "3    rs2943772       G       C     440.77          .   \n",
       "4  rs139651072       T       C     216.77          .   \n",
       "\n",
       "                                            VCF_INFO      VCF_FORMAT  \\\n",
       "0  ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....  GT:AD:DP:GQ:PL   \n",
       "1  ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...  GT:AD:DP:GQ:PL   \n",
       "2  AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...  GT:AD:DP:GQ:PL   \n",
       "3  ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...  GT:AD:DP:GQ:PL   \n",
       "4  ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...  GT:AD:DP:GQ:PL   \n",
       "\n",
       "                    VCF_SAMPLE_NAME  \n",
       "0  0/1:1266,552:1820:99:319,0,12285  \n",
       "1    0/1:306,267:574:99:7992,0,9864  \n",
       "2             1/1:0,6:7:18:272,18,0  \n",
       "3           1/1:0,14:14:36:469,36,0  \n",
       "4          0/1:10,8:18:99:245,0,289  \n",
       "\n",
       "[5 rows x 183 columns]"
      ]
     },
     "execution_count": 16,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df.head()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* A função `head()` mostra as primeiras 5 linhas do `DataFrame`, mas é possível especificar quantas você quer ver pondo um valor inteiro dentro dos `()`, como:"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 17,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
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       "\n",
       "    .dataframe tbody tr th {\n",
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       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>Func.refGene</th>\n",
       "      <th>Gene.refGene</th>\n",
       "      <th>GeneDetail.refGene</th>\n",
       "      <th>ExonicFunc.refGene</th>\n",
       "      <th>AAChange.refGene</th>\n",
       "      <th>...</th>\n",
       "      <th>VCF_CHR</th>\n",
       "      <th>VCF_POS</th>\n",
       "      <th>VCF_ID</th>\n",
       "      <th>VCF_REF</th>\n",
       "      <th>VCF_ALT</th>\n",
       "      <th>VCF_QUAL_2</th>\n",
       "      <th>VCF_FILTER</th>\n",
       "      <th>VCF_INFO</th>\n",
       "      <th>VCF_FORMAT</th>\n",
       "      <th>VCF_SAMPLE_NAME</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>89622938</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>NM_001126049:c.-694T&gt;G</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>290.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1266,552:1820:99:319,0,12285</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>89623142</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>NM_001126049:c.-898G&gt;A</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>7963.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:306,267:574:99:7992,0,9864</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623861</td>\n",
       "      <td>89623861</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>splicing</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304717:exon1:c.154+1T&gt;-;NM_001304717:exo...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623860</td>\n",
       "      <td>rs71022512</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>234.80</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,6:7:18:272,18,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>89623901</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>exonic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>nonsynonymous SNV</td>\n",
       "      <td>PTEN:NM_001304717:exon2:c.194G&gt;C:p.C65S</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>rs2943772</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>440.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,14:14:36:469,36,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>89654121</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>rs139651072</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>216.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:10,8:18:99:245,0,289</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>5</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190</td>\n",
       "      <td>89654190</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190</td>\n",
       "      <td>rs181780682</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>29.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.714;AC=1;AF=0.5;AN=2;BaseQRankSum=0.36...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:5,2:7:58:58,0,172</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>6</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89711576</td>\n",
       "      <td>89711576</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89711576</td>\n",
       "      <td>rs141005791</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>96.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.21...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:5,4:9:99:125,0,133</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>7</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89718057</td>\n",
       "      <td>89718057</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89718057</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>759.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.324;AC=1;AF=0.5;AN=2;BaseQRankSum=0.83...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:11,23:34:99:788,0,283</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>8</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720548</td>\n",
       "      <td>89720548</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720548</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>1491.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.467;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.5...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:42,48:90:99:1520,0,1330</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>9</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720634</td>\n",
       "      <td>89720634</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720633</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>1805.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.601;DP=689;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:443,192:689:99:1843,0,6330</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>10</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720907</td>\n",
       "      <td>89720907</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720907</td>\n",
       "      <td>rs555895</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>10070.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.555;AC=1;AF=0.5;AN=2;BaseQRankSum=6.08...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:397,318:717:99:10099,0,11475</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>11</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89721094</td>\n",
       "      <td>89721094</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89721094</td>\n",
       "      <td>rs2736627</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>3289.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=92;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,92:92:99:3318,259,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>12</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725294</td>\n",
       "      <td>89725294</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304718:c.*65delT;NM_001304717:c.*65delT;...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89725293</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>9448.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.417;DP=2406;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1610,625:2406:99:9486,0,34365</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>13</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582</td>\n",
       "      <td>89725582</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304718:c.*353C&gt;T;NM_001304717:c.*353C&gt;T;...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582</td>\n",
       "      <td>rs181234898</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>201.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.526;AC=1;AF=0.5;AN=2;BaseQRankSum=0.31...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:10,9:19:99:230,0,266</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>14</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32889968</td>\n",
       "      <td>32889968</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32889968</td>\n",
       "      <td>rs206119</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>3707.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=98;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,98:98:99:3736,289,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>15</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32899567</td>\n",
       "      <td>32899567</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32899567</td>\n",
       "      <td>rs11571613</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>634.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.286;AC=1;AF=0.5;AN=2;BaseQRankSum=0.96...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:8,20:28:99:663,0,227</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>16</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32899878</td>\n",
       "      <td>32899878</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32899878</td>\n",
       "      <td>rs206071</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>633.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=19;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,19:19:51:662,51,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>17</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32905220</td>\n",
       "      <td>32905220</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32905219</td>\n",
       "      <td>.</td>\n",
       "      <td>AT</td>\n",
       "      <td>A</td>\n",
       "      <td>2225.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=4.537;DP=630;Exc...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:413,156:630:99:2263,0,8472</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>18</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32905265</td>\n",
       "      <td>32905265</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32905265</td>\n",
       "      <td>rs206073</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>14280.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=0.995;AC=2;AF=1;AN=2;BaseQRankSum=-0.511...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:2,388:392:99:14309,1038,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>19</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32907536</td>\n",
       "      <td>32907536</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32907535</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>4200.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=3.476;DP=1016;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:669,272:1016:99:4238,0,14585</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>20</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32911888</td>\n",
       "      <td>32911888</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>exonic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>synonymous SNV</td>\n",
       "      <td>BRCA2:NM_000059:exon11:c.3396A&gt;G:p.K1132K</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32911888</td>\n",
       "      <td>rs1801406</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>30677.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.511;AC=1;AF=0.5;AN=2;BaseQRankSum=1.67...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1393,1332:2730:99:30706,0,32616</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>21</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32912299</td>\n",
       "      <td>32912299</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>exonic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>synonymous SNV</td>\n",
       "      <td>BRCA2:NM_000059:exon11:c.3807T&gt;C:p.V1269V</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32912299</td>\n",
       "      <td>rs543304</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>35335.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.503;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1388,1370:2759:99:35364,0,36070</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>22</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32913055</td>\n",
       "      <td>32913055</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>exonic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>synonymous SNV</td>\n",
       "      <td>BRCA2:NM_000059:exon11:c.4563A&gt;G:p.L1521L</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32913055</td>\n",
       "      <td>rs206075</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>53264.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=2013;Dels=0;Exces...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,2010:2013:99:53293,3847,0</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>23 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr     Start       End Ref Alt Func.refGene Gene.refGene  \\\n",
       "0   chr10  89622938  89622938   A   C         UTR5         KLLN   \n",
       "1   chr10  89623142  89623142   C   T         UTR5         KLLN   \n",
       "2   chr10  89623861  89623861   T   -     splicing         PTEN   \n",
       "3   chr10  89623901  89623901   G   C       exonic         PTEN   \n",
       "4   chr10  89654121  89654121   T   C     intronic         PTEN   \n",
       "5   chr10  89654190  89654190   G   A     intronic         PTEN   \n",
       "6   chr10  89711576  89711576   C   T     intronic         PTEN   \n",
       "7   chr10  89718057  89718057   A   G     intronic         PTEN   \n",
       "8   chr10  89720548  89720548   A   G     intronic         PTEN   \n",
       "9   chr10  89720634  89720634   T   -     intronic         PTEN   \n",
       "10  chr10  89720907  89720907   T   G     intronic         PTEN   \n",
       "11  chr10  89721094  89721094   C   T     intronic         PTEN   \n",
       "12  chr10  89725294  89725294   T   -         UTR3         PTEN   \n",
       "13  chr10  89725582  89725582   C   T         UTR3         PTEN   \n",
       "14  chr13  32889968  32889968   G   A     intronic        BRCA2   \n",
       "15  chr13  32899567  32899567   G   C     intronic        BRCA2   \n",
       "16  chr13  32899878  32899878   C   T     intronic        BRCA2   \n",
       "17  chr13  32905220  32905220   T   -     intronic        BRCA2   \n",
       "18  chr13  32905265  32905265   G   A     intronic        BRCA2   \n",
       "19  chr13  32907536  32907536   T   -     intronic        BRCA2   \n",
       "20  chr13  32911888  32911888   A   G       exonic        BRCA2   \n",
       "21  chr13  32912299  32912299   T   C       exonic        BRCA2   \n",
       "22  chr13  32913055  32913055   A   G       exonic        BRCA2   \n",
       "\n",
       "                                   GeneDetail.refGene ExonicFunc.refGene  \\\n",
       "0                              NM_001126049:c.-694T>G                  .   \n",
       "1                              NM_001126049:c.-898G>A                  .   \n",
       "2   NM_001304717:exon1:c.154+1T>-;NM_001304717:exo...                  .   \n",
       "3                                                   .  nonsynonymous SNV   \n",
       "4                                                   .                  .   \n",
       "5                                                   .                  .   \n",
       "6                                                   .                  .   \n",
       "7                                                   .                  .   \n",
       "8                                                   .                  .   \n",
       "9                                                   .                  .   \n",
       "10                                                  .                  .   \n",
       "11                                                  .                  .   \n",
       "12  NM_001304718:c.*65delT;NM_001304717:c.*65delT;...                  .   \n",
       "13  NM_001304718:c.*353C>T;NM_001304717:c.*353C>T;...                  .   \n",
       "14                                                  .                  .   \n",
       "15                                                  .                  .   \n",
       "16                                                  .                  .   \n",
       "17                                                  .                  .   \n",
       "18                                                  .                  .   \n",
       "19                                                  .                  .   \n",
       "20                                                  .     synonymous SNV   \n",
       "21                                                  .     synonymous SNV   \n",
       "22                                                  .     synonymous SNV   \n",
       "\n",
       "                             AAChange.refGene  ... VCF_CHR   VCF_POS  \\\n",
       "0                                           .  ...   chr10  89622938   \n",
       "1                                           .  ...   chr10  89623142   \n",
       "2                                           .  ...   chr10  89623860   \n",
       "3     PTEN:NM_001304717:exon2:c.194G>C:p.C65S  ...   chr10  89623901   \n",
       "4                                           .  ...   chr10  89654121   \n",
       "5                                           .  ...   chr10  89654190   \n",
       "6                                           .  ...   chr10  89711576   \n",
       "7                                           .  ...   chr10  89718057   \n",
       "8                                           .  ...   chr10  89720548   \n",
       "9                                           .  ...   chr10  89720633   \n",
       "10                                          .  ...   chr10  89720907   \n",
       "11                                          .  ...   chr10  89721094   \n",
       "12                                          .  ...   chr10  89725293   \n",
       "13                                          .  ...   chr10  89725582   \n",
       "14                                          .  ...   chr13  32889968   \n",
       "15                                          .  ...   chr13  32899567   \n",
       "16                                          .  ...   chr13  32899878   \n",
       "17                                          .  ...   chr13  32905219   \n",
       "18                                          .  ...   chr13  32905265   \n",
       "19                                          .  ...   chr13  32907535   \n",
       "20  BRCA2:NM_000059:exon11:c.3396A>G:p.K1132K  ...   chr13  32911888   \n",
       "21  BRCA2:NM_000059:exon11:c.3807T>C:p.V1269V  ...   chr13  32912299   \n",
       "22  BRCA2:NM_000059:exon11:c.4563A>G:p.L1521L  ...   chr13  32913055   \n",
       "\n",
       "         VCF_ID VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "0             .       A       C     290.77          .   \n",
       "1             .       C       T    7963.77          .   \n",
       "2    rs71022512      CT       C     234.80          .   \n",
       "3     rs2943772       G       C     440.77          .   \n",
       "4   rs139651072       T       C     216.77          .   \n",
       "5   rs181780682       G       A      29.77          .   \n",
       "6   rs141005791       C       T      96.77          .   \n",
       "7             .       A       G     759.77          .   \n",
       "8             .       A       G    1491.77          .   \n",
       "9             .      CT       C    1805.73          .   \n",
       "10     rs555895       T       G   10070.80          .   \n",
       "11    rs2736627       C       T    3289.77          .   \n",
       "12            .      CT       C    9448.73          .   \n",
       "13  rs181234898       C       T     201.77          .   \n",
       "14     rs206119       G       A    3707.77          .   \n",
       "15   rs11571613       G       C     634.77          .   \n",
       "16     rs206071       C       T     633.77          .   \n",
       "17            .      AT       A    2225.73          .   \n",
       "18     rs206073       G       A   14280.80          .   \n",
       "19            .      CT       C    4200.73          .   \n",
       "20    rs1801406       A       G   30677.80          .   \n",
       "21     rs543304       T       C   35335.80          .   \n",
       "22     rs206075       A       G   53264.80          .   \n",
       "\n",
       "                                             VCF_INFO      VCF_FORMAT  \\\n",
       "0   ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....  GT:AD:DP:GQ:PL   \n",
       "1   ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...  GT:AD:DP:GQ:PL   \n",
       "2   AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...  GT:AD:DP:GQ:PL   \n",
       "3   ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...  GT:AD:DP:GQ:PL   \n",
       "4   ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...  GT:AD:DP:GQ:PL   \n",
       "5   ABHet=0.714;AC=1;AF=0.5;AN=2;BaseQRankSum=0.36...  GT:AD:DP:GQ:PL   \n",
       "6   ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.21...  GT:AD:DP:GQ:PL   \n",
       "7   ABHet=0.324;AC=1;AF=0.5;AN=2;BaseQRankSum=0.83...  GT:AD:DP:GQ:PL   \n",
       "8   ABHet=0.467;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.5...  GT:AD:DP:GQ:PL   \n",
       "9   AC=1;AF=0.5;AN=2;BaseQRankSum=-0.601;DP=689;Ex...  GT:AD:DP:GQ:PL   \n",
       "10  ABHet=0.555;AC=1;AF=0.5;AN=2;BaseQRankSum=6.08...  GT:AD:DP:GQ:PL   \n",
       "11  ABHom=1;AC=2;AF=1;AN=2;DB;DP=92;Dels=0;ExcessH...  GT:AD:DP:GQ:PL   \n",
       "12  AC=1;AF=0.5;AN=2;BaseQRankSum=0.417;DP=2406;Ex...  GT:AD:DP:GQ:PL   \n",
       "13  ABHet=0.526;AC=1;AF=0.5;AN=2;BaseQRankSum=0.31...  GT:AD:DP:GQ:PL   \n",
       "14  ABHom=1;AC=2;AF=1;AN=2;DB;DP=98;Dels=0;ExcessH...  GT:AD:DP:GQ:PL   \n",
       "15  ABHet=0.286;AC=1;AF=0.5;AN=2;BaseQRankSum=0.96...  GT:AD:DP:GQ:PL   \n",
       "16  ABHom=1;AC=2;AF=1;AN=2;DB;DP=19;Dels=0;ExcessH...  GT:AD:DP:GQ:PL   \n",
       "17  AC=1;AF=0.5;AN=2;BaseQRankSum=4.537;DP=630;Exc...  GT:AD:DP:GQ:PL   \n",
       "18  ABHom=0.995;AC=2;AF=1;AN=2;BaseQRankSum=-0.511...  GT:AD:DP:GQ:PL   \n",
       "19  AC=1;AF=0.5;AN=2;BaseQRankSum=3.476;DP=1016;Ex...  GT:AD:DP:GQ:PL   \n",
       "20  ABHet=0.511;AC=1;AF=0.5;AN=2;BaseQRankSum=1.67...  GT:AD:DP:GQ:PL   \n",
       "21  ABHet=0.503;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....  GT:AD:DP:GQ:PL   \n",
       "22  ABHom=1;AC=2;AF=1;AN=2;DB;DP=2013;Dels=0;Exces...  GT:AD:DP:GQ:PL   \n",
       "\n",
       "                        VCF_SAMPLE_NAME  \n",
       "0      0/1:1266,552:1820:99:319,0,12285  \n",
       "1        0/1:306,267:574:99:7992,0,9864  \n",
       "2                 1/1:0,6:7:18:272,18,0  \n",
       "3               1/1:0,14:14:36:469,36,0  \n",
       "4              0/1:10,8:18:99:245,0,289  \n",
       "5                 0/1:5,2:7:58:58,0,172  \n",
       "6                0/1:5,4:9:99:125,0,133  \n",
       "7             0/1:11,23:34:99:788,0,283  \n",
       "8           0/1:42,48:90:99:1520,0,1330  \n",
       "9        0/1:443,192:689:99:1843,0,6330  \n",
       "10     0/1:397,318:717:99:10099,0,11475  \n",
       "11            1/1:0,92:92:99:3318,259,0  \n",
       "12    0/1:1610,625:2406:99:9486,0,34365  \n",
       "13             0/1:10,9:19:99:230,0,266  \n",
       "14            1/1:0,98:98:99:3736,289,0  \n",
       "15             0/1:8,20:28:99:663,0,227  \n",
       "16              1/1:0,19:19:51:662,51,0  \n",
       "17       0/1:413,156:630:99:2263,0,8472  \n",
       "18        1/1:2,388:392:99:14309,1038,0  \n",
       "19     0/1:669,272:1016:99:4238,0,14585  \n",
       "20  0/1:1393,1332:2730:99:30706,0,32616  \n",
       "21  0/1:1388,1370:2759:99:35364,0,36070  \n",
       "22      1/1:0,2010:2013:99:53293,3847,0  \n",
       "\n",
       "[23 rows x 183 columns]"
      ]
     },
     "execution_count": 17,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df.head(23)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Do mesmo jeito pode se usar a função `tail()`"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 18,
   "metadata": {},
   "outputs": [
    {
     "data": {
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       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>Func.refGene</th>\n",
       "      <th>Gene.refGene</th>\n",
       "      <th>GeneDetail.refGene</th>\n",
       "      <th>ExonicFunc.refGene</th>\n",
       "      <th>AAChange.refGene</th>\n",
       "      <th>...</th>\n",
       "      <th>VCF_CHR</th>\n",
       "      <th>VCF_POS</th>\n",
       "      <th>VCF_ID</th>\n",
       "      <th>VCF_REF</th>\n",
       "      <th>VCF_ALT</th>\n",
       "      <th>VCF_QUAL_2</th>\n",
       "      <th>VCF_FILTER</th>\n",
       "      <th>VCF_INFO</th>\n",
       "      <th>VCF_FORMAT</th>\n",
       "      <th>VCF_SAMPLE_NAME</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>87</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41258136</td>\n",
       "      <td>41258136</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41258135</td>\n",
       "      <td>.</td>\n",
       "      <td>TA</td>\n",
       "      <td>T</td>\n",
       "      <td>203.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-1.019;DP=69;Exc...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:36,24:69:99:241,0,424</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>88</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1206797</td>\n",
       "      <td>1206797</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>STK11</td>\n",
       "      <td>NM_000455:c.-116del-</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1206796</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>2222.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=1.767;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:604,172:776:99:1:0.5:2260,0,15656:245,359,...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>89</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1219444</td>\n",
       "      <td>1219444</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>STK11</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1219444</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>672.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.307;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.8...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:39,88:127:99:701,0,458</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>90</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1219451</td>\n",
       "      <td>1219451</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>STK11</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1219451</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>213.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.654;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.6...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:117,62:179:99:242,0,1041</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>91</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418</td>\n",
       "      <td>1221418</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>STK11</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418</td>\n",
       "      <td>.</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>25.78</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.417;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.9...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:108,151:263:54:54,0,1020</td>\n",
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       "  </tbody>\n",
       "</table>\n",
       "<p>5 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr     Start       End Ref Alt Func.refGene Gene.refGene  \\\n",
       "87  chr17  41258136  41258136   A   -     intronic        BRCA1   \n",
       "88  chr19   1206797   1206797   T   -         UTR5        STK11   \n",
       "89  chr19   1219444   1219444   C   G     intronic        STK11   \n",
       "90  chr19   1219451   1219451   C   G     intronic        STK11   \n",
       "91  chr19   1221418   1221418   T   G     intronic        STK11   \n",
       "\n",
       "      GeneDetail.refGene ExonicFunc.refGene AAChange.refGene  ... VCF_CHR  \\\n",
       "87                     .                  .                .  ...   chr17   \n",
       "88  NM_000455:c.-116del-                  .                .  ...   chr19   \n",
       "89                     .                  .                .  ...   chr19   \n",
       "90                     .                  .                .  ...   chr19   \n",
       "91                     .                  .                .  ...   chr19   \n",
       "\n",
       "     VCF_POS VCF_ID VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "87  41258135      .      TA       T     203.73          .   \n",
       "88   1206796      .      CT       C    2222.73          .   \n",
       "89   1219444      .       C       G     672.77          .   \n",
       "90   1219451      .       C       G     213.77          .   \n",
       "91   1221418      .       T       G      25.78          .   \n",
       "\n",
       "                                             VCF_INFO  \\\n",
       "87  AC=1;AF=0.5;AN=2;BaseQRankSum=-1.019;DP=69;Exc...   \n",
       "88  AC=1;AF=0.5;AN=2;BaseQRankSum=1.767;ClippingRa...   \n",
       "89  ABHet=0.307;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.8...   \n",
       "90  ABHet=0.654;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.6...   \n",
       "91  ABHet=0.417;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.9...   \n",
       "\n",
       "                             VCF_FORMAT  \\\n",
       "87                       GT:AD:DP:GQ:PL   \n",
       "88  GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "89                       GT:AD:DP:GQ:PL   \n",
       "90                       GT:AD:DP:GQ:PL   \n",
       "91                       GT:AD:DP:GQ:PL   \n",
       "\n",
       "                                      VCF_SAMPLE_NAME  \n",
       "87                          0/1:36,24:69:99:241,0,424  \n",
       "88  0/1:604,172:776:99:1:0.5:2260,0,15656:245,359,...  \n",
       "89                         0/1:39,88:127:99:701,0,458  \n",
       "90                       0/1:117,62:179:99:242,0,1041  \n",
       "91                       0/1:108,151:263:54:54,0,1020  \n",
       "\n",
       "[5 rows x 183 columns]"
      ]
     },
     "execution_count": 18,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df.tail()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 19,
   "metadata": {},
   "outputs": [
    {
     "data": {
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       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
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       "      <th>VCF_QUAL_2</th>\n",
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       "      <th>VCF_INFO</th>\n",
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       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>90</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1219451</td>\n",
       "      <td>1219451</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>STK11</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1219451</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>213.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.654;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.6...</td>\n",
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       "      <td>0/1:117,62:179:99:242,0,1041</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>91</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418</td>\n",
       "      <td>1221418</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>STK11</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418</td>\n",
       "      <td>.</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>25.78</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.417;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.9...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:108,151:263:54:54,0,1020</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>2 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr    Start      End Ref Alt Func.refGene Gene.refGene  \\\n",
       "90  chr19  1219451  1219451   C   G     intronic        STK11   \n",
       "91  chr19  1221418  1221418   T   G     intronic        STK11   \n",
       "\n",
       "   GeneDetail.refGene ExonicFunc.refGene AAChange.refGene  ... VCF_CHR  \\\n",
       "90                  .                  .                .  ...   chr19   \n",
       "91                  .                  .                .  ...   chr19   \n",
       "\n",
       "    VCF_POS VCF_ID VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "90  1219451      .       C       G     213.77          .   \n",
       "91  1221418      .       T       G      25.78          .   \n",
       "\n",
       "                                             VCF_INFO      VCF_FORMAT  \\\n",
       "90  ABHet=0.654;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.6...  GT:AD:DP:GQ:PL   \n",
       "91  ABHet=0.417;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.9...  GT:AD:DP:GQ:PL   \n",
       "\n",
       "                 VCF_SAMPLE_NAME  \n",
       "90  0/1:117,62:179:99:242,0,1041  \n",
       "91  0/1:108,151:263:54:54,0,1020  \n",
       "\n",
       "[2 rows x 183 columns]"
      ]
     },
     "execution_count": 19,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df.tail(2)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* É possível fazer um *slicing* de linhas a serem visualização usando os valores dos índices (sendo o último valor não incluso)."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 20,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>Func.refGene</th>\n",
       "      <th>Gene.refGene</th>\n",
       "      <th>GeneDetail.refGene</th>\n",
       "      <th>ExonicFunc.refGene</th>\n",
       "      <th>AAChange.refGene</th>\n",
       "      <th>...</th>\n",
       "      <th>VCF_CHR</th>\n",
       "      <th>VCF_POS</th>\n",
       "      <th>VCF_ID</th>\n",
       "      <th>VCF_REF</th>\n",
       "      <th>VCF_ALT</th>\n",
       "      <th>VCF_QUAL_2</th>\n",
       "      <th>VCF_FILTER</th>\n",
       "      <th>VCF_INFO</th>\n",
       "      <th>VCF_FORMAT</th>\n",
       "      <th>VCF_SAMPLE_NAME</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>89623901</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>exonic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>nonsynonymous SNV</td>\n",
       "      <td>PTEN:NM_001304717:exon2:c.194G&gt;C:p.C65S</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>rs2943772</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>440.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,14:14:36:469,36,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>89654121</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>rs139651072</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>216.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:10,8:18:99:245,0,289</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>5</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190</td>\n",
       "      <td>89654190</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190</td>\n",
       "      <td>rs181780682</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>29.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.714;AC=1;AF=0.5;AN=2;BaseQRankSum=0.36...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:5,2:7:58:58,0,172</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>6</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89711576</td>\n",
       "      <td>89711576</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89711576</td>\n",
       "      <td>rs141005791</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>96.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.21...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:5,4:9:99:125,0,133</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>4 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "     Chr     Start       End Ref Alt Func.refGene Gene.refGene  \\\n",
       "3  chr10  89623901  89623901   G   C       exonic         PTEN   \n",
       "4  chr10  89654121  89654121   T   C     intronic         PTEN   \n",
       "5  chr10  89654190  89654190   G   A     intronic         PTEN   \n",
       "6  chr10  89711576  89711576   C   T     intronic         PTEN   \n",
       "\n",
       "  GeneDetail.refGene ExonicFunc.refGene  \\\n",
       "3                  .  nonsynonymous SNV   \n",
       "4                  .                  .   \n",
       "5                  .                  .   \n",
       "6                  .                  .   \n",
       "\n",
       "                          AAChange.refGene  ... VCF_CHR   VCF_POS  \\\n",
       "3  PTEN:NM_001304717:exon2:c.194G>C:p.C65S  ...   chr10  89623901   \n",
       "4                                        .  ...   chr10  89654121   \n",
       "5                                        .  ...   chr10  89654190   \n",
       "6                                        .  ...   chr10  89711576   \n",
       "\n",
       "        VCF_ID VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "3    rs2943772       G       C     440.77          .   \n",
       "4  rs139651072       T       C     216.77          .   \n",
       "5  rs181780682       G       A      29.77          .   \n",
       "6  rs141005791       C       T      96.77          .   \n",
       "\n",
       "                                            VCF_INFO      VCF_FORMAT  \\\n",
       "3  ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...  GT:AD:DP:GQ:PL   \n",
       "4  ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...  GT:AD:DP:GQ:PL   \n",
       "5  ABHet=0.714;AC=1;AF=0.5;AN=2;BaseQRankSum=0.36...  GT:AD:DP:GQ:PL   \n",
       "6  ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.21...  GT:AD:DP:GQ:PL   \n",
       "\n",
       "            VCF_SAMPLE_NAME  \n",
       "3   1/1:0,14:14:36:469,36,0  \n",
       "4  0/1:10,8:18:99:245,0,289  \n",
       "5     0/1:5,2:7:58:58,0,172  \n",
       "6    0/1:5,4:9:99:125,0,133  \n",
       "\n",
       "[4 rows x 183 columns]"
      ]
     },
     "execution_count": 20,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df[3:7]"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Para saber quais as colunas presentes no `DataFrame` basta usar o método `columns`, que ele retorna algo parecido com uma lista contendo todos os nomes de colunas."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 21,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "Index(['Chr', 'Start', 'End', 'Ref', 'Alt', 'Func.refGene', 'Gene.refGene',\n",
       "       'GeneDetail.refGene', 'ExonicFunc.refGene', 'AAChange.refGene',\n",
       "       ...\n",
       "       'VCF_CHR', 'VCF_POS', 'VCF_ID', 'VCF_REF', 'VCF_ALT', 'VCF_QUAL_2',\n",
       "       'VCF_FILTER', 'VCF_INFO', 'VCF_FORMAT', 'VCF_SAMPLE_NAME'],\n",
       "      dtype='object', length=183)"
      ]
     },
     "execution_count": 21,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df.columns"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Dá para percerber que ele não te mostra tudo, se quiser mesmo ver tudo use o método `values` em cima do método `columns`."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 22,
   "metadata": {
    "scrolled": false
   },
   "outputs": [
    {
     "data": {
      "text/plain": [
       "array(['Chr', 'Start', 'End', 'Ref', 'Alt', 'Func.refGene',\n",
       "       'Gene.refGene', 'GeneDetail.refGene', 'ExonicFunc.refGene',\n",
       "       'AAChange.refGene', 'ABRaOM_HomozygousALT', 'ABRaOM_Hemizygous',\n",
       "       'ABRaOM_Allele_number', 'ABRaOM_Allele_ALT_count',\n",
       "       'ABRaOM_Frequencies', 'esp6500siv2_all', '1000g2015aug_all',\n",
       "       'ExAC_ALL', 'ExAC_AFR', 'ExAC_AMR', 'ExAC_EAS', 'ExAC_FIN',\n",
       "       'ExAC_NFE', 'ExAC_OTH', 'ExAC_SAS', 'CLNALLELEID', 'CLNDN',\n",
       "       'CLNDISDB', 'CLNREVSTAT', 'CLNSIG', 'avsift', 'SIFT_score',\n",
       "       'SIFT_converted_rankscore', 'SIFT_pred', 'Polyphen2_HDIV_score',\n",
       "       'Polyphen2_HDIV_rankscore', 'Polyphen2_HDIV_pred',\n",
       "       'Polyphen2_HVAR_score', 'Polyphen2_HVAR_rankscore',\n",
       "       'Polyphen2_HVAR_pred', 'LRT_score', 'LRT_converted_rankscore',\n",
       "       'LRT_pred', 'MutationTaster_score',\n",
       "       'MutationTaster_converted_rankscore', 'MutationTaster_pred',\n",
       "       'MutationAssessor_score', 'MutationAssessor_score_rankscore',\n",
       "       'MutationAssessor_pred', 'FATHMM_score',\n",
       "       'FATHMM_converted_rankscore', 'FATHMM_pred', 'PROVEAN_score',\n",
       "       'PROVEAN_converted_rankscore', 'PROVEAN_pred', 'VEST3_score',\n",
       "       'VEST3_rankscore', 'MetaSVM_score', 'MetaSVM_rankscore',\n",
       "       'MetaSVM_pred', 'MetaLR_score', 'MetaLR_rankscore', 'MetaLR_pred',\n",
       "       'M-CAP_score', 'M-CAP_rankscore', 'M-CAP_pred', 'CADD_raw',\n",
       "       'CADD_raw_rankscore', 'CADD_phred', 'DANN_score', 'DANN_rankscore',\n",
       "       'fathmm-MKL_coding_score', 'fathmm-MKL_coding_rankscore',\n",
       "       'fathmm-MKL_coding_pred', 'Eigen_coding_or_noncoding', 'Eigen-raw',\n",
       "       'Eigen-PC-raw', 'GenoCanyon_score', 'GenoCanyon_score_rankscore',\n",
       "       'integrated_fitCons_score', 'integrated_fitCons_score_rankscore',\n",
       "       'integrated_confidence_value', 'GERP++_RS', 'GERP++_RS_rankscore',\n",
       "       'phyloP100way_vertebrate', 'phyloP100way_vertebrate_rankscore',\n",
       "       'phyloP20way_mammalian', 'phyloP20way_mammalian_rankscore',\n",
       "       'phastCons100way_vertebrate',\n",
       "       'phastCons100way_vertebrate_rankscore', 'phastCons20way_mammalian',\n",
       "       'phastCons20way_mammalian_rankscore', 'SiPhy_29way_logOdds',\n",
       "       'SiPhy_29way_logOdds_rankscore', 'Interpro_domain', 'GTEx_V6_gene',\n",
       "       'GTEx_V6_tissue', 'snp138', 'segdup', 'mce46way', 'cosmic70',\n",
       "       'nci60', 'avsnp150', 'AF', 'AF_popmax', 'AF_male', 'AF_female',\n",
       "       'AF_raw', 'AF_afr', 'AF_sas', 'AF_amr', 'AF_eas', 'AF_nfe',\n",
       "       'AF_fin', 'AF_asj', 'AF_oth', 'non_topmed_AF_popmax',\n",
       "       'non_neuro_AF_popmax', 'non_cancer_AF_popmax',\n",
       "       'controls_AF_popmax', 'AF.1', 'AF_popmax.1', 'AF_male.1',\n",
       "       'AF_female.1', 'AF_raw.1', 'AF_afr.1', 'AF_sas.1', 'AF_amr.1',\n",
       "       'AF_eas.1', 'AF_nfe.1', 'AF_fin.1', 'AF_asj.1', 'AF_oth.1',\n",
       "       'non_topmed_AF_popmax.1', 'non_neuro_AF_popmax.1',\n",
       "       'non_cancer_AF_popmax.1', 'controls_AF_popmax.1',\n",
       "       'InterVar(automated)', 'PVS1', 'PS1', 'PS2', 'PS3', 'PS4', 'PM1',\n",
       "       'PM2', 'PM3', 'PM4', 'PM5', 'PM6', 'PP1', 'PP2', 'PP3', 'PP4',\n",
       "       'PP5', 'BA1', 'BS1', 'BS2', 'BS3', 'BS4', 'BP1', 'BP2', 'BP3',\n",
       "       'BP4', 'BP5', 'BP6', 'BP7', 'bed', 'bed2',\n",
       "       'HIAE_EXOME_FREQUENCY(722)', 'HIAE_EXOME_OCCURRENCES(722)',\n",
       "       'Otherinfo', 'VCF_QUAL_1', 'VCF_DP', 'VCF_CHR', 'VCF_POS',\n",
       "       'VCF_ID', 'VCF_REF', 'VCF_ALT', 'VCF_QUAL_2', 'VCF_FILTER',\n",
       "       'VCF_INFO', 'VCF_FORMAT', 'VCF_SAMPLE_NAME'], dtype=object)"
      ]
     },
     "execution_count": 22,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df.columns.values"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Se você quer visualizar dados de uma determinada coluna, existem duas formas de fazer isso, onde é retorna uma `Series`:"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 23,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "0     chr10\n",
       "1     chr10\n",
       "2     chr10\n",
       "3     chr10\n",
       "4     chr10\n",
       "      ...  \n",
       "87    chr17\n",
       "88    chr19\n",
       "89    chr19\n",
       "90    chr19\n",
       "91    chr19\n",
       "Name: Chr, Length: 92, dtype: object"
      ]
     },
     "execution_count": 23,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df.Chr"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 24,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "0     chr10\n",
       "1     chr10\n",
       "2     chr10\n",
       "3     chr10\n",
       "4     chr10\n",
       "      ...  \n",
       "87    chr17\n",
       "88    chr19\n",
       "89    chr19\n",
       "90    chr19\n",
       "91    chr19\n",
       "Name: Chr, Length: 92, dtype: object"
      ]
     },
     "execution_count": 24,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df[\"Chr\"]"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 25,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "pandas.core.series.Series"
      ]
     },
     "execution_count": 25,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "type(multianno_df[\"Chr\"])"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* É mais indicado selecionar uma coluna usando aspas já que houver algum caracter estranho no nome da coluna ele dará erro na seleção;\n",
    "* Para selecionar múltiplas colunas basta criar uma lista contendo as colunas que você quer visualizar."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 26,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>89622938</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>89623142</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623861</td>\n",
       "      <td>89623861</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>89623901</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>89654121</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>...</th>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>87</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41258136</td>\n",
       "      <td>41258136</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>88</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1206797</td>\n",
       "      <td>1206797</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>89</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1219444</td>\n",
       "      <td>1219444</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>90</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1219451</td>\n",
       "      <td>1219451</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>91</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418</td>\n",
       "      <td>1221418</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>92 rows × 3 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr     Start       End\n",
       "0   chr10  89622938  89622938\n",
       "1   chr10  89623142  89623142\n",
       "2   chr10  89623861  89623861\n",
       "3   chr10  89623901  89623901\n",
       "4   chr10  89654121  89654121\n",
       "..    ...       ...       ...\n",
       "87  chr17  41258136  41258136\n",
       "88  chr19   1206797   1206797\n",
       "89  chr19   1219444   1219444\n",
       "90  chr19   1219451   1219451\n",
       "91  chr19   1221418   1221418\n",
       "\n",
       "[92 rows x 3 columns]"
      ]
     },
     "execution_count": 26,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "col_list = ['Chr', 'Start', 'End']\n",
    "multianno_df[col_list]"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 27,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>89622938</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>89623142</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623861</td>\n",
       "      <td>89623861</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>89623901</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>89654121</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>...</th>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>87</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41258136</td>\n",
       "      <td>41258136</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>88</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1206797</td>\n",
       "      <td>1206797</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>89</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1219444</td>\n",
       "      <td>1219444</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>90</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1219451</td>\n",
       "      <td>1219451</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>91</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418</td>\n",
       "      <td>1221418</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>92 rows × 3 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr     Start       End\n",
       "0   chr10  89622938  89622938\n",
       "1   chr10  89623142  89623142\n",
       "2   chr10  89623861  89623861\n",
       "3   chr10  89623901  89623901\n",
       "4   chr10  89654121  89654121\n",
       "..    ...       ...       ...\n",
       "87  chr17  41258136  41258136\n",
       "88  chr19   1206797   1206797\n",
       "89  chr19   1219444   1219444\n",
       "90  chr19   1219451   1219451\n",
       "91  chr19   1221418   1221418\n",
       "\n",
       "[92 rows x 3 columns]"
      ]
     },
     "execution_count": 27,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df[['Chr', 'Start', 'End']]"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Algumas funções de estatísticas básicas podem ser calculadas nas colunas como `max()`, `min()`, `mean()`, `median()`"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 28,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "0    0.000\n",
       "1    0.003\n",
       "2    0.339\n",
       "3    0.350\n",
       "4    0.001\n",
       "Name: HIAE_EXOME_FREQUENCY(722), dtype: float64"
      ]
     },
     "execution_count": 28,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Substituir \".\" por \"0\"\n",
    "multianno_df[\"HIAE_EXOME_FREQUENCY(722)\"] = multianno_df[\"HIAE_EXOME_FREQUENCY(722)\"].apply(\n",
    "    lambda x: \"0\" if x == \".\" else x)\n",
    "# Deixar 3 casas decimais\n",
    "multianno_df[\"HIAE_EXOME_FREQUENCY(722)\"] = multianno_df[\"HIAE_EXOME_FREQUENCY(722)\"].astype(float).round(3)\n",
    "multianno_df[\"HIAE_EXOME_FREQUENCY(722)\"].head()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 29,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "0.0"
      ]
     },
     "execution_count": 29,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df[\"HIAE_EXOME_FREQUENCY(722)\"].min()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 30,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "0.938"
      ]
     },
     "execution_count": 30,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df[\"HIAE_EXOME_FREQUENCY(722)\"].max()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 31,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "0.2149130434782609"
      ]
     },
     "execution_count": 31,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df[\"HIAE_EXOME_FREQUENCY(722)\"].mean()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 32,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "0.074"
      ]
     },
     "execution_count": 32,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df[\"HIAE_EXOME_FREQUENCY(722)\"].median()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 33,
   "metadata": {
    "scrolled": false
   },
   "outputs": [
    {
     "data": {
      "text/plain": [
       "count    92.000000\n",
       "mean      0.214913\n",
       "std       0.294639\n",
       "min       0.000000\n",
       "25%       0.001000\n",
       "50%       0.074000\n",
       "75%       0.350500\n",
       "max       0.938000\n",
       "Name: HIAE_EXOME_FREQUENCY(722), dtype: float64"
      ]
     },
     "execution_count": 33,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df[\"HIAE_EXOME_FREQUENCY(722)\"].describe()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Para a contagem de valores em uma coluna, podemos usar a função `value_counts()`"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 34,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       ".                                               47\n",
       "Benign                                          37\n",
       "Likely_benign                                    3\n",
       "Conflicting_interpretations_of_pathogenicity     2\n",
       "Pathogenic                                       1\n",
       "drug_response                                    1\n",
       "Uncertain_significance                           1\n",
       "Name: CLNSIG, dtype: int64"
      ]
     },
     "execution_count": 34,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df[\"CLNSIG\"].value_counts()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Mais operações em colunas únicas (`Series`) podem ser vistas [aqui](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.Series.html)\n",
    "\n",
    "### Escrevendo CSV, Excel\n",
    "\n",
    "* Para escrever o `CSV` basta usar a função `to_csv()`, manipulando os parâmetros necessários."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 35,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
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       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>89622938</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>89623142</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623861</td>\n",
       "      <td>89623861</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>89623901</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>89654121</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "</div>"
      ],
      "text/plain": [
       "     Chr     Start       End\n",
       "0  chr10  89622938  89622938\n",
       "1  chr10  89623142  89623142\n",
       "2  chr10  89623861  89623861\n",
       "3  chr10  89623901  89623901\n",
       "4  chr10  89654121  89654121"
      ]
     },
     "execution_count": 35,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "output = multianno_df[[\"Chr\", \"Start\", \"End\"]]\n",
    "output.head()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 36,
   "metadata": {},
   "outputs": [],
   "source": [
    "output.to_csv('multianno_filtrado.csv', index=False)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Para criar um excel simples é da mesma forma, mas dessa vez usando a função `to_excel()` e seus parâmetros.\n",
    "\n",
    "> Aqui é preciso ter a biblioteca `Openpyxl` instalada."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 37,
   "metadata": {
    "scrolled": true
   },
   "outputs": [],
   "source": [
    "output.to_excel('multianno_filtrado.xlsx', sheet_name=\"bed\", index=False)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Mas se você quer ir além disso e criar um arquivo excel com diferentes abas, por exemplo usando 2 `DataFrames` cada um com sua aba:"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 38,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
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       "        text-align: right;\n",
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       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>Func.refGene</th>\n",
       "      <th>Gene.refGene</th>\n",
       "      <th>GeneDetail.refGene</th>\n",
       "      <th>ExonicFunc.refGene</th>\n",
       "      <th>AAChange.refGene</th>\n",
       "      <th>...</th>\n",
       "      <th>VCF_CHR</th>\n",
       "      <th>VCF_POS</th>\n",
       "      <th>VCF_ID</th>\n",
       "      <th>VCF_REF</th>\n",
       "      <th>VCF_ALT</th>\n",
       "      <th>VCF_QUAL_2</th>\n",
       "      <th>VCF_FILTER</th>\n",
       "      <th>VCF_INFO</th>\n",
       "      <th>VCF_FORMAT</th>\n",
       "      <th>VCF_SAMPLE_NAME</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>89622938</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>NM_001126049:c.-694T&gt;G</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>290.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1266,552:1820:99:319,0,12285</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>89623142</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>NM_001126049:c.-898G&gt;A</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>7963.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:306,267:574:99:7992,0,9864</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623861</td>\n",
       "      <td>89623861</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>splicing</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304717:exon1:c.154+1T&gt;-;NM_001304717:exo...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623860</td>\n",
       "      <td>rs71022512</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>234.80</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,6:7:18:272,18,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>89623901</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>exonic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>nonsynonymous SNV</td>\n",
       "      <td>PTEN:NM_001304717:exon2:c.194G&gt;C:p.C65S</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>rs2943772</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>440.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,14:14:36:469,36,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>89654121</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>rs139651072</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>216.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:10,8:18:99:245,0,289</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>5</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190</td>\n",
       "      <td>89654190</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190</td>\n",
       "      <td>rs181780682</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>29.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.714;AC=1;AF=0.5;AN=2;BaseQRankSum=0.36...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:5,2:7:58:58,0,172</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>6</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89711576</td>\n",
       "      <td>89711576</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89711576</td>\n",
       "      <td>rs141005791</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>96.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.21...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:5,4:9:99:125,0,133</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>7</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89718057</td>\n",
       "      <td>89718057</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89718057</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>759.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.324;AC=1;AF=0.5;AN=2;BaseQRankSum=0.83...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:11,23:34:99:788,0,283</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>8</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720548</td>\n",
       "      <td>89720548</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720548</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>1491.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.467;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.5...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:42,48:90:99:1520,0,1330</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>9</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720634</td>\n",
       "      <td>89720634</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720633</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>1805.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.601;DP=689;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:443,192:689:99:1843,0,6330</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>10</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720907</td>\n",
       "      <td>89720907</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720907</td>\n",
       "      <td>rs555895</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>10070.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.555;AC=1;AF=0.5;AN=2;BaseQRankSum=6.08...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:397,318:717:99:10099,0,11475</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>11</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89721094</td>\n",
       "      <td>89721094</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89721094</td>\n",
       "      <td>rs2736627</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>3289.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=92;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,92:92:99:3318,259,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>12</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725294</td>\n",
       "      <td>89725294</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304718:c.*65delT;NM_001304717:c.*65delT;...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89725293</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>9448.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.417;DP=2406;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1610,625:2406:99:9486,0,34365</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>13</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582</td>\n",
       "      <td>89725582</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304718:c.*353C&gt;T;NM_001304717:c.*353C&gt;T;...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582</td>\n",
       "      <td>rs181234898</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>201.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.526;AC=1;AF=0.5;AN=2;BaseQRankSum=0.31...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:10,9:19:99:230,0,266</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>14</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32889968</td>\n",
       "      <td>32889968</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32889968</td>\n",
       "      <td>rs206119</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>3707.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=98;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,98:98:99:3736,289,0</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>15 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr     Start       End Ref Alt Func.refGene Gene.refGene  \\\n",
       "0   chr10  89622938  89622938   A   C         UTR5         KLLN   \n",
       "1   chr10  89623142  89623142   C   T         UTR5         KLLN   \n",
       "2   chr10  89623861  89623861   T   -     splicing         PTEN   \n",
       "3   chr10  89623901  89623901   G   C       exonic         PTEN   \n",
       "4   chr10  89654121  89654121   T   C     intronic         PTEN   \n",
       "5   chr10  89654190  89654190   G   A     intronic         PTEN   \n",
       "6   chr10  89711576  89711576   C   T     intronic         PTEN   \n",
       "7   chr10  89718057  89718057   A   G     intronic         PTEN   \n",
       "8   chr10  89720548  89720548   A   G     intronic         PTEN   \n",
       "9   chr10  89720634  89720634   T   -     intronic         PTEN   \n",
       "10  chr10  89720907  89720907   T   G     intronic         PTEN   \n",
       "11  chr10  89721094  89721094   C   T     intronic         PTEN   \n",
       "12  chr10  89725294  89725294   T   -         UTR3         PTEN   \n",
       "13  chr10  89725582  89725582   C   T         UTR3         PTEN   \n",
       "14  chr13  32889968  32889968   G   A     intronic        BRCA2   \n",
       "\n",
       "                                   GeneDetail.refGene ExonicFunc.refGene  \\\n",
       "0                              NM_001126049:c.-694T>G                  .   \n",
       "1                              NM_001126049:c.-898G>A                  .   \n",
       "2   NM_001304717:exon1:c.154+1T>-;NM_001304717:exo...                  .   \n",
       "3                                                   .  nonsynonymous SNV   \n",
       "4                                                   .                  .   \n",
       "5                                                   .                  .   \n",
       "6                                                   .                  .   \n",
       "7                                                   .                  .   \n",
       "8                                                   .                  .   \n",
       "9                                                   .                  .   \n",
       "10                                                  .                  .   \n",
       "11                                                  .                  .   \n",
       "12  NM_001304718:c.*65delT;NM_001304717:c.*65delT;...                  .   \n",
       "13  NM_001304718:c.*353C>T;NM_001304717:c.*353C>T;...                  .   \n",
       "14                                                  .                  .   \n",
       "\n",
       "                           AAChange.refGene  ... VCF_CHR   VCF_POS  \\\n",
       "0                                         .  ...   chr10  89622938   \n",
       "1                                         .  ...   chr10  89623142   \n",
       "2                                         .  ...   chr10  89623860   \n",
       "3   PTEN:NM_001304717:exon2:c.194G>C:p.C65S  ...   chr10  89623901   \n",
       "4                                         .  ...   chr10  89654121   \n",
       "5                                         .  ...   chr10  89654190   \n",
       "6                                         .  ...   chr10  89711576   \n",
       "7                                         .  ...   chr10  89718057   \n",
       "8                                         .  ...   chr10  89720548   \n",
       "9                                         .  ...   chr10  89720633   \n",
       "10                                        .  ...   chr10  89720907   \n",
       "11                                        .  ...   chr10  89721094   \n",
       "12                                        .  ...   chr10  89725293   \n",
       "13                                        .  ...   chr10  89725582   \n",
       "14                                        .  ...   chr13  32889968   \n",
       "\n",
       "         VCF_ID VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "0             .       A       C     290.77          .   \n",
       "1             .       C       T    7963.77          .   \n",
       "2    rs71022512      CT       C     234.80          .   \n",
       "3     rs2943772       G       C     440.77          .   \n",
       "4   rs139651072       T       C     216.77          .   \n",
       "5   rs181780682       G       A      29.77          .   \n",
       "6   rs141005791       C       T      96.77          .   \n",
       "7             .       A       G     759.77          .   \n",
       "8             .       A       G    1491.77          .   \n",
       "9             .      CT       C    1805.73          .   \n",
       "10     rs555895       T       G   10070.80          .   \n",
       "11    rs2736627       C       T    3289.77          .   \n",
       "12            .      CT       C    9448.73          .   \n",
       "13  rs181234898       C       T     201.77          .   \n",
       "14     rs206119       G       A    3707.77          .   \n",
       "\n",
       "                                             VCF_INFO      VCF_FORMAT  \\\n",
       "0   ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....  GT:AD:DP:GQ:PL   \n",
       "1   ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...  GT:AD:DP:GQ:PL   \n",
       "2   AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...  GT:AD:DP:GQ:PL   \n",
       "3   ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...  GT:AD:DP:GQ:PL   \n",
       "4   ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...  GT:AD:DP:GQ:PL   \n",
       "5   ABHet=0.714;AC=1;AF=0.5;AN=2;BaseQRankSum=0.36...  GT:AD:DP:GQ:PL   \n",
       "6   ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.21...  GT:AD:DP:GQ:PL   \n",
       "7   ABHet=0.324;AC=1;AF=0.5;AN=2;BaseQRankSum=0.83...  GT:AD:DP:GQ:PL   \n",
       "8   ABHet=0.467;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.5...  GT:AD:DP:GQ:PL   \n",
       "9   AC=1;AF=0.5;AN=2;BaseQRankSum=-0.601;DP=689;Ex...  GT:AD:DP:GQ:PL   \n",
       "10  ABHet=0.555;AC=1;AF=0.5;AN=2;BaseQRankSum=6.08...  GT:AD:DP:GQ:PL   \n",
       "11  ABHom=1;AC=2;AF=1;AN=2;DB;DP=92;Dels=0;ExcessH...  GT:AD:DP:GQ:PL   \n",
       "12  AC=1;AF=0.5;AN=2;BaseQRankSum=0.417;DP=2406;Ex...  GT:AD:DP:GQ:PL   \n",
       "13  ABHet=0.526;AC=1;AF=0.5;AN=2;BaseQRankSum=0.31...  GT:AD:DP:GQ:PL   \n",
       "14  ABHom=1;AC=2;AF=1;AN=2;DB;DP=98;Dels=0;ExcessH...  GT:AD:DP:GQ:PL   \n",
       "\n",
       "                      VCF_SAMPLE_NAME  \n",
       "0    0/1:1266,552:1820:99:319,0,12285  \n",
       "1      0/1:306,267:574:99:7992,0,9864  \n",
       "2               1/1:0,6:7:18:272,18,0  \n",
       "3             1/1:0,14:14:36:469,36,0  \n",
       "4            0/1:10,8:18:99:245,0,289  \n",
       "5               0/1:5,2:7:58:58,0,172  \n",
       "6              0/1:5,4:9:99:125,0,133  \n",
       "7           0/1:11,23:34:99:788,0,283  \n",
       "8         0/1:42,48:90:99:1520,0,1330  \n",
       "9      0/1:443,192:689:99:1843,0,6330  \n",
       "10   0/1:397,318:717:99:10099,0,11475  \n",
       "11          1/1:0,92:92:99:3318,259,0  \n",
       "12  0/1:1610,625:2406:99:9486,0,34365  \n",
       "13           0/1:10,9:19:99:230,0,266  \n",
       "14          1/1:0,98:98:99:3736,289,0  \n",
       "\n",
       "[15 rows x 183 columns]"
      ]
     },
     "execution_count": 38,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "df1 = multianno_df.head(15)\n",
    "df1"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 39,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
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       "<style scoped>\n",
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       "\n",
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       "        vertical-align: top;\n",
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       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>Func.refGene</th>\n",
       "      <th>Gene.refGene</th>\n",
       "      <th>GeneDetail.refGene</th>\n",
       "      <th>ExonicFunc.refGene</th>\n",
       "      <th>AAChange.refGene</th>\n",
       "      <th>...</th>\n",
       "      <th>VCF_CHR</th>\n",
       "      <th>VCF_POS</th>\n",
       "      <th>VCF_ID</th>\n",
       "      <th>VCF_REF</th>\n",
       "      <th>VCF_ALT</th>\n",
       "      <th>VCF_QUAL_2</th>\n",
       "      <th>VCF_FILTER</th>\n",
       "      <th>VCF_INFO</th>\n",
       "      <th>VCF_FORMAT</th>\n",
       "      <th>VCF_SAMPLE_NAME</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>77</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7579644</td>\n",
       "      <td>7579659</td>\n",
       "      <td>CCCCAGCCCTCCAGGT</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7579643</td>\n",
       "      <td>rs146534833</td>\n",
       "      <td>CCCCCAGCCCTCCAGGT</td>\n",
       "      <td>C</td>\n",
       "      <td>122210.00</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=2;AF=1;AN=2;DB;DP=1405;ExcessHet=3.0103;FS=...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,924:1405:99:122247,2780,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>78</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7579801</td>\n",
       "      <td>7579801</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>TP53</td>\n",
       "      <td>NM_001126118:c.-232C&gt;G</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7579801</td>\n",
       "      <td>rs1642785</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>9509.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=0.651;AC=2;AF=1;AN=2;BaseQRankSum=16.02;...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:203,378:582:99:9538,522,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>79</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7590593</td>\n",
       "      <td>7590593</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53;WRAP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7590593</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>87.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.749;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.3...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:188,63:251:99:116,0,652</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>80</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7590601</td>\n",
       "      <td>7590601</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53;WRAP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7590601</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>339.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.585;AC=1;AF=0.5;AN=2;BaseQRankSum=-2.0...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:172,122:294:99:368,0,1128</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>81</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7590607</td>\n",
       "      <td>7590607</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53;WRAP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7590607</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>41.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.704;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.0...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:226,95:321:70:70,0,2914</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>82</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41196822</td>\n",
       "      <td>41196824</td>\n",
       "      <td>TTT</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>NM_007300:c.*873_*871delAAA;NM_007297:c.*873_*...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41196821</td>\n",
       "      <td>.</td>\n",
       "      <td>CTTT</td>\n",
       "      <td>C</td>\n",
       "      <td>84.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.689;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:109,19:128:99:1:0.5:122,0,3145:69,40,12,7:...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>83</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41197940</td>\n",
       "      <td>41197940</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41197939</td>\n",
       "      <td>.</td>\n",
       "      <td>AT</td>\n",
       "      <td>A</td>\n",
       "      <td>1932.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=3.135;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:60,107:167:99:1:0.5:1970,0,654:2,58,33,74:...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>84</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41247621</td>\n",
       "      <td>41247621</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41247620</td>\n",
       "      <td>.</td>\n",
       "      <td>CA</td>\n",
       "      <td>C</td>\n",
       "      <td>197.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.097;DP=38;Exc...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:24,13:38:99:235,0,434</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>85</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41256075</td>\n",
       "      <td>41256075</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41256074</td>\n",
       "      <td>.</td>\n",
       "      <td>CA</td>\n",
       "      <td>C</td>\n",
       "      <td>932.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.364;DP=201;Exc...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:128,67:201:99:970,0,2530</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>86</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41256088</td>\n",
       "      <td>41256088</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41256087</td>\n",
       "      <td>.</td>\n",
       "      <td>GA</td>\n",
       "      <td>G</td>\n",
       "      <td>357.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.38;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:83,28:111:99:1:0.5:395,0,2036:83,0,28,0:70...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>87</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41258136</td>\n",
       "      <td>41258136</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41258135</td>\n",
       "      <td>.</td>\n",
       "      <td>TA</td>\n",
       "      <td>T</td>\n",
       "      <td>203.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-1.019;DP=69;Exc...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:36,24:69:99:241,0,424</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>88</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1206797</td>\n",
       "      <td>1206797</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>STK11</td>\n",
       "      <td>NM_000455:c.-116del-</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1206796</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>2222.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=1.767;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:604,172:776:99:1:0.5:2260,0,15656:245,359,...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>89</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1219444</td>\n",
       "      <td>1219444</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>STK11</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1219444</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>672.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.307;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.8...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:39,88:127:99:701,0,458</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>90</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1219451</td>\n",
       "      <td>1219451</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>STK11</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1219451</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>213.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.654;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.6...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:117,62:179:99:242,0,1041</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>91</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418</td>\n",
       "      <td>1221418</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>STK11</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418</td>\n",
       "      <td>.</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>25.78</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.417;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.9...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:108,151:263:54:54,0,1020</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>15 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr     Start       End               Ref Alt Func.refGene Gene.refGene  \\\n",
       "77  chr17   7579644   7579659  CCCCAGCCCTCCAGGT   -     intronic         TP53   \n",
       "78  chr17   7579801   7579801                 G   C         UTR5         TP53   \n",
       "79  chr17   7590593   7590593                 A   C     intronic  TP53;WRAP53   \n",
       "80  chr17   7590601   7590601                 A   C     intronic  TP53;WRAP53   \n",
       "81  chr17   7590607   7590607                 A   C     intronic  TP53;WRAP53   \n",
       "82  chr17  41196822  41196824               TTT   -         UTR3        BRCA1   \n",
       "83  chr17  41197940  41197940                 T   -     intronic        BRCA1   \n",
       "84  chr17  41247621  41247621                 A   -     intronic        BRCA1   \n",
       "85  chr17  41256075  41256075                 A   -     intronic        BRCA1   \n",
       "86  chr17  41256088  41256088                 A   -     intronic        BRCA1   \n",
       "87  chr17  41258136  41258136                 A   -     intronic        BRCA1   \n",
       "88  chr19   1206797   1206797                 T   -         UTR5        STK11   \n",
       "89  chr19   1219444   1219444                 C   G     intronic        STK11   \n",
       "90  chr19   1219451   1219451                 C   G     intronic        STK11   \n",
       "91  chr19   1221418   1221418                 T   G     intronic        STK11   \n",
       "\n",
       "                                   GeneDetail.refGene ExonicFunc.refGene  \\\n",
       "77                                                  .                  .   \n",
       "78                             NM_001126118:c.-232C>G                  .   \n",
       "79                                                  .                  .   \n",
       "80                                                  .                  .   \n",
       "81                                                  .                  .   \n",
       "82  NM_007300:c.*873_*871delAAA;NM_007297:c.*873_*...                  .   \n",
       "83                                                  .                  .   \n",
       "84                                                  .                  .   \n",
       "85                                                  .                  .   \n",
       "86                                                  .                  .   \n",
       "87                                                  .                  .   \n",
       "88                               NM_000455:c.-116del-                  .   \n",
       "89                                                  .                  .   \n",
       "90                                                  .                  .   \n",
       "91                                                  .                  .   \n",
       "\n",
       "   AAChange.refGene  ... VCF_CHR   VCF_POS       VCF_ID            VCF_REF  \\\n",
       "77                .  ...   chr17   7579643  rs146534833  CCCCCAGCCCTCCAGGT   \n",
       "78                .  ...   chr17   7579801    rs1642785                  G   \n",
       "79                .  ...   chr17   7590593            .                  A   \n",
       "80                .  ...   chr17   7590601            .                  A   \n",
       "81                .  ...   chr17   7590607            .                  A   \n",
       "82                .  ...   chr17  41196821            .               CTTT   \n",
       "83                .  ...   chr17  41197939            .                 AT   \n",
       "84                .  ...   chr17  41247620            .                 CA   \n",
       "85                .  ...   chr17  41256074            .                 CA   \n",
       "86                .  ...   chr17  41256087            .                 GA   \n",
       "87                .  ...   chr17  41258135            .                 TA   \n",
       "88                .  ...   chr19   1206796            .                 CT   \n",
       "89                .  ...   chr19   1219444            .                  C   \n",
       "90                .  ...   chr19   1219451            .                  C   \n",
       "91                .  ...   chr19   1221418            .                  T   \n",
       "\n",
       "   VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "77       C  122210.00          .   \n",
       "78       C    9509.77          .   \n",
       "79       C      87.77          .   \n",
       "80       C     339.77          .   \n",
       "81       C      41.77          .   \n",
       "82       C      84.73          .   \n",
       "83       A    1932.73          .   \n",
       "84       C     197.73          .   \n",
       "85       C     932.73          .   \n",
       "86       G     357.73          .   \n",
       "87       T     203.73          .   \n",
       "88       C    2222.73          .   \n",
       "89       G     672.77          .   \n",
       "90       G     213.77          .   \n",
       "91       G      25.78          .   \n",
       "\n",
       "                                             VCF_INFO  \\\n",
       "77  AC=2;AF=1;AN=2;DB;DP=1405;ExcessHet=3.0103;FS=...   \n",
       "78  ABHom=0.651;AC=2;AF=1;AN=2;BaseQRankSum=16.02;...   \n",
       "79  ABHet=0.749;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.3...   \n",
       "80  ABHet=0.585;AC=1;AF=0.5;AN=2;BaseQRankSum=-2.0...   \n",
       "81  ABHet=0.704;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.0...   \n",
       "82  AC=1;AF=0.5;AN=2;BaseQRankSum=0.689;ClippingRa...   \n",
       "83  AC=1;AF=0.5;AN=2;BaseQRankSum=3.135;ClippingRa...   \n",
       "84  AC=1;AF=0.5;AN=2;BaseQRankSum=-0.097;DP=38;Exc...   \n",
       "85  AC=1;AF=0.5;AN=2;BaseQRankSum=0.364;DP=201;Exc...   \n",
       "86  AC=1;AF=0.5;AN=2;BaseQRankSum=-0.38;ClippingRa...   \n",
       "87  AC=1;AF=0.5;AN=2;BaseQRankSum=-1.019;DP=69;Exc...   \n",
       "88  AC=1;AF=0.5;AN=2;BaseQRankSum=1.767;ClippingRa...   \n",
       "89  ABHet=0.307;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.8...   \n",
       "90  ABHet=0.654;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.6...   \n",
       "91  ABHet=0.417;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.9...   \n",
       "\n",
       "                             VCF_FORMAT  \\\n",
       "77                       GT:AD:DP:GQ:PL   \n",
       "78                       GT:AD:DP:GQ:PL   \n",
       "79                       GT:AD:DP:GQ:PL   \n",
       "80                       GT:AD:DP:GQ:PL   \n",
       "81                       GT:AD:DP:GQ:PL   \n",
       "82  GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "83  GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "84                       GT:AD:DP:GQ:PL   \n",
       "85                       GT:AD:DP:GQ:PL   \n",
       "86  GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "87                       GT:AD:DP:GQ:PL   \n",
       "88  GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "89                       GT:AD:DP:GQ:PL   \n",
       "90                       GT:AD:DP:GQ:PL   \n",
       "91                       GT:AD:DP:GQ:PL   \n",
       "\n",
       "                                      VCF_SAMPLE_NAME  \n",
       "77                    1/1:0,924:1405:99:122247,2780,0  \n",
       "78                      1/1:203,378:582:99:9538,522,0  \n",
       "79                        0/1:188,63:251:99:116,0,652  \n",
       "80                      0/1:172,122:294:99:368,0,1128  \n",
       "81                        0/1:226,95:321:70:70,0,2914  \n",
       "82  0/1:109,19:128:99:1:0.5:122,0,3145:69,40,12,7:...  \n",
       "83  0/1:60,107:167:99:1:0.5:1970,0,654:2,58,33,74:...  \n",
       "84                          0/1:24,13:38:99:235,0,434  \n",
       "85                       0/1:128,67:201:99:970,0,2530  \n",
       "86  0/1:83,28:111:99:1:0.5:395,0,2036:83,0,28,0:70...  \n",
       "87                          0/1:36,24:69:99:241,0,424  \n",
       "88  0/1:604,172:776:99:1:0.5:2260,0,15656:245,359,...  \n",
       "89                         0/1:39,88:127:99:701,0,458  \n",
       "90                       0/1:117,62:179:99:242,0,1041  \n",
       "91                       0/1:108,151:263:54:54,0,1020  \n",
       "\n",
       "[15 rows x 183 columns]"
      ]
     },
     "execution_count": 39,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "df2 = multianno_df.tail(15)\n",
    "df2"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 40,
   "metadata": {},
   "outputs": [],
   "source": [
    "with pd.ExcelWriter('multianno_filtrado_2abas.xlsx') as writer:\n",
    "    df1.to_excel(writer, sheet_name=\"DataFrame1\", index=False)\n",
    "    df2.to_excel(writer, sheet_name=\"DataFrame2\", index=False)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "\n",
    "\n",
    "---\n",
    "\n",
    "# 2. Manipulando `DataFrame`s\n",
    "\n",
    "### Manipulando índices\n",
    "\n",
    "* Existem métodos para selecionar linhas e colunas, sendo as mais indicadas `iloc` e `loc`.\n",
    "* A primeira forma (`iloc`) é baseada na indexação para seleção da posição:"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 41,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "Chr                                                            chr10\n",
       "Start                                                       89622938\n",
       "End                                                         89622938\n",
       "Ref                                                                A\n",
       "Alt                                                                C\n",
       "                                         ...                        \n",
       "VCF_QUAL_2                                                    290.77\n",
       "VCF_FILTER                                                         .\n",
       "VCF_INFO           ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....\n",
       "VCF_FORMAT                                            GT:AD:DP:GQ:PL\n",
       "VCF_SAMPLE_NAME                     0/1:1266,552:1820:99:319,0,12285\n",
       "Name: 0, Length: 183, dtype: object"
      ]
     },
     "execution_count": 41,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Selecionando a primeira linha, transformando as colunas em índices de uma Series\n",
    "multianno_df.iloc[0]"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 42,
   "metadata": {},
   "outputs": [
    {
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      ],
      "text/plain": [
       "     Chr     Start       End Ref Alt Func.refGene Gene.refGene  \\\n",
       "0  chr10  89622938  89622938   A   C         UTR5         KLLN   \n",
       "\n",
       "       GeneDetail.refGene ExonicFunc.refGene AAChange.refGene  ... VCF_CHR  \\\n",
       "0  NM_001126049:c.-694T>G                  .                .  ...   chr10   \n",
       "\n",
       "    VCF_POS VCF_ID VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "0  89622938      .       A       C     290.77          .   \n",
       "\n",
       "                                            VCF_INFO      VCF_FORMAT  \\\n",
       "0  ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....  GT:AD:DP:GQ:PL   \n",
       "\n",
       "                    VCF_SAMPLE_NAME  \n",
       "0  0/1:1266,552:1820:99:319,0,12285  \n",
       "\n",
       "[1 rows x 183 columns]"
      ]
     },
     "execution_count": 42,
     "metadata": {},
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    }
   ],
   "source": [
    "# Selecionando a primeira linha, mas na forma de DataFrame\n",
    "multianno_df.iloc[[0]]"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 43,
   "metadata": {},
   "outputs": [
    {
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       "     Chr     Start       End Ref Alt Func.refGene Gene.refGene  \\\n",
       "0  chr10  89622938  89622938   A   C         UTR5         KLLN   \n",
       "1  chr10  89623142  89623142   C   T         UTR5         KLLN   \n",
       "\n",
       "       GeneDetail.refGene ExonicFunc.refGene AAChange.refGene  ... VCF_CHR  \\\n",
       "0  NM_001126049:c.-694T>G                  .                .  ...   chr10   \n",
       "1  NM_001126049:c.-898G>A                  .                .  ...   chr10   \n",
       "\n",
       "    VCF_POS VCF_ID VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "0  89622938      .       A       C     290.77          .   \n",
       "1  89623142      .       C       T    7963.77          .   \n",
       "\n",
       "                                            VCF_INFO      VCF_FORMAT  \\\n",
       "0  ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....  GT:AD:DP:GQ:PL   \n",
       "1  ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...  GT:AD:DP:GQ:PL   \n",
       "\n",
       "                    VCF_SAMPLE_NAME  \n",
       "0  0/1:1266,552:1820:99:319,0,12285  \n",
       "1    0/1:306,267:574:99:7992,0,9864  \n",
       "\n",
       "[2 rows x 183 columns]"
      ]
     },
     "execution_count": 43,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Selecionando as duas primeiras linhas\n",
    "multianno_df.iloc[[0, 1]]"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 44,
   "metadata": {},
   "outputs": [
    {
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      "text/plain": [
       "     Chr     Start       End Ref Alt Func.refGene Gene.refGene  \\\n",
       "0  chr10  89622938  89622938   A   C         UTR5         KLLN   \n",
       "1  chr10  89623142  89623142   C   T         UTR5         KLLN   \n",
       "\n",
       "       GeneDetail.refGene ExonicFunc.refGene AAChange.refGene  ... VCF_CHR  \\\n",
       "0  NM_001126049:c.-694T>G                  .                .  ...   chr10   \n",
       "1  NM_001126049:c.-898G>A                  .                .  ...   chr10   \n",
       "\n",
       "    VCF_POS VCF_ID VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "0  89622938      .       A       C     290.77          .   \n",
       "1  89623142      .       C       T    7963.77          .   \n",
       "\n",
       "                                            VCF_INFO      VCF_FORMAT  \\\n",
       "0  ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....  GT:AD:DP:GQ:PL   \n",
       "1  ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...  GT:AD:DP:GQ:PL   \n",
       "\n",
       "                    VCF_SAMPLE_NAME  \n",
       "0  0/1:1266,552:1820:99:319,0,12285  \n",
       "1    0/1:306,267:574:99:7992,0,9864  \n",
       "\n",
       "[2 rows x 183 columns]"
      ]
     },
     "execution_count": 44,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Selecionando as duas primeiras linhas usando slicing\n",
    "multianno_df.iloc[:2]"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 45,
   "metadata": {},
   "outputs": [
    {
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       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623861</td>\n",
       "      <td>89623861</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>splicing</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304717:exon1:c.154+1T&gt;-;NM_001304717:exo...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623860</td>\n",
       "      <td>rs71022512</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>234.80</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,6:7:18:272,18,0</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>2 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "     Chr     Start       End Ref Alt Func.refGene Gene.refGene  \\\n",
       "0  chr10  89622938  89622938   A   C         UTR5         KLLN   \n",
       "2  chr10  89623861  89623861   T   -     splicing         PTEN   \n",
       "\n",
       "                                  GeneDetail.refGene ExonicFunc.refGene  \\\n",
       "0                             NM_001126049:c.-694T>G                  .   \n",
       "2  NM_001304717:exon1:c.154+1T>-;NM_001304717:exo...                  .   \n",
       "\n",
       "  AAChange.refGene  ... VCF_CHR   VCF_POS      VCF_ID VCF_REF VCF_ALT  \\\n",
       "0                .  ...   chr10  89622938           .       A       C   \n",
       "2                .  ...   chr10  89623860  rs71022512      CT       C   \n",
       "\n",
       "  VCF_QUAL_2 VCF_FILTER                                           VCF_INFO  \\\n",
       "0     290.77          .  ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....   \n",
       "2     234.80          .  AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...   \n",
       "\n",
       "       VCF_FORMAT                   VCF_SAMPLE_NAME  \n",
       "0  GT:AD:DP:GQ:PL  0/1:1266,552:1820:99:319,0,12285  \n",
       "2  GT:AD:DP:GQ:PL             1/1:0,6:7:18:272,18,0  \n",
       "\n",
       "[2 rows x 183 columns]"
      ]
     },
     "execution_count": 45,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Selecionando linhas especificas a partir de uma lista de booleanos\n",
    "ex = multianno_df.iloc[:3] # filtro, senao ele pedirá 92 booleanos\n",
    "ex.iloc[[True, False, True]]"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 46,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "89622938"
      ]
     },
     "execution_count": 46,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Selecionando o valor presente na primeira linha da segunda coluna\n",
    "multianno_df.iloc[0, 1]"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 47,
   "metadata": {},
   "outputs": [
    {
     "data": {
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       "      <th>2</th>\n",
       "      <td>89623861</td>\n",
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      "text/plain": [
       "      Start Ref Alt\n",
       "0  89622938   A   C\n",
       "2  89623861   T   -"
      ]
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     "execution_count": 47,
     "metadata": {},
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   ],
   "source": [
    "# Selecionando linhas e colunas por lista de listas\n",
    "multianno_df.iloc[[0, 2], [1, 3, 4]]"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 48,
   "metadata": {},
   "outputs": [
    {
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       "      <td>89623142</td>\n",
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       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623861</td>\n",
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      "text/plain": [
       "     Chr     Start       End\n",
       "1  chr10  89623142  89623142\n",
       "2  chr10  89623861  89623861"
      ]
     },
     "execution_count": 48,
     "metadata": {},
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   ],
   "source": [
    "# Selecionando linhas e colunas usando slicing\n",
    "multianno_df.iloc[1:3, 0:3] # linha 3 não é incluida"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Enquanto a segunda (`loc`) é baseada no nome das colunas ou booleanos:"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 49,
   "metadata": {},
   "outputs": [
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       "     Chr     Start       End Ref Alt Func.refGene Gene.refGene  \\\n",
       "0  chr10  89622938  89622938   A   C         UTR5         KLLN   \n",
       "\n",
       "       GeneDetail.refGene ExonicFunc.refGene AAChange.refGene  ... VCF_CHR  \\\n",
       "0  NM_001126049:c.-694T>G                  .                .  ...   chr10   \n",
       "\n",
       "    VCF_POS VCF_ID VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "0  89622938      .       A       C     290.77          .   \n",
       "\n",
       "                                            VCF_INFO      VCF_FORMAT  \\\n",
       "0  ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....  GT:AD:DP:GQ:PL   \n",
       "\n",
       "                    VCF_SAMPLE_NAME  \n",
       "0  0/1:1266,552:1820:99:319,0,12285  \n",
       "\n",
       "[1 rows x 183 columns]"
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     },
     "execution_count": 49,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Como os índices são numericos, a seleção de uma linha fica igual ao iloc\n",
    "multianno_df.loc[[0]] # nao precisa de dois [[]], foi apenas para resultar em dum DataFrame"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 50,
   "metadata": {
    "scrolled": true
   },
   "outputs": [
    {
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       "            Chr       End Ref Alt Func.refGene Gene.refGene  \\\n",
       "Start                                                         \n",
       "89623142  chr10  89623142   C   T         UTR5         KLLN   \n",
       "\n",
       "              GeneDetail.refGene ExonicFunc.refGene AAChange.refGene  \\\n",
       "Start                                                                  \n",
       "89623142  NM_001126049:c.-898G>A                  .                .   \n",
       "\n",
       "         ABRaOM_HomozygousALT  ... VCF_CHR   VCF_POS VCF_ID VCF_REF VCF_ALT  \\\n",
       "Start                          ...                                            \n",
       "89623142                    2  ...   chr10  89623142      .       C       T   \n",
       "\n",
       "         VCF_QUAL_2 VCF_FILTER  \\\n",
       "Start                            \n",
       "89623142    7963.77          .   \n",
       "\n",
       "                                                   VCF_INFO      VCF_FORMAT  \\\n",
       "Start                                                                         \n",
       "89623142  ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...  GT:AD:DP:GQ:PL   \n",
       "\n",
       "                         VCF_SAMPLE_NAME  \n",
       "Start                                     \n",
       "89623142  0/1:306,267:574:99:7992,0,9864  \n",
       "\n",
       "[1 rows x 182 columns]"
      ]
     },
     "execution_count": 50,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# No caso de um indice personalizado é possível selecionar linhas via loc\n",
    "ex = multianno_df.set_index('Start')\n",
    "ex.loc[[89623142]]"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 51,
   "metadata": {},
   "outputs": [
    {
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       "      <th>89623901</th>\n",
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       "      <td>561</td>\n",
       "      <td>...</td>\n",
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       "      <td>.</td>\n",
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       "      <td>1/1:0,14:14:36:469,36,0</td>\n",
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       "<p>2 rows × 182 columns</p>\n",
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      ],
      "text/plain": [
       "            Chr       End Ref Alt Func.refGene Gene.refGene  \\\n",
       "Start                                                         \n",
       "89623142  chr10  89623142   C   T         UTR5         KLLN   \n",
       "89623901  chr10  89623901   G   C       exonic         PTEN   \n",
       "\n",
       "              GeneDetail.refGene ExonicFunc.refGene  \\\n",
       "Start                                                 \n",
       "89623142  NM_001126049:c.-898G>A                  .   \n",
       "89623901                       .  nonsynonymous SNV   \n",
       "\n",
       "                                 AAChange.refGene ABRaOM_HomozygousALT  ...  \\\n",
       "Start                                                                   ...   \n",
       "89623142                                        .                    2  ...   \n",
       "89623901  PTEN:NM_001304717:exon2:c.194G>C:p.C65S                  561  ...   \n",
       "\n",
       "         VCF_CHR   VCF_POS     VCF_ID VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "Start                                                                         \n",
       "89623142   chr10  89623142          .       C       T    7963.77          .   \n",
       "89623901   chr10  89623901  rs2943772       G       C     440.77          .   \n",
       "\n",
       "                                                   VCF_INFO      VCF_FORMAT  \\\n",
       "Start                                                                         \n",
       "89623142  ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...  GT:AD:DP:GQ:PL   \n",
       "89623901  ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...  GT:AD:DP:GQ:PL   \n",
       "\n",
       "                         VCF_SAMPLE_NAME  \n",
       "Start                                     \n",
       "89623142  0/1:306,267:574:99:7992,0,9864  \n",
       "89623901         1/1:0,14:14:36:469,36,0  \n",
       "\n",
       "[2 rows x 182 columns]"
      ]
     },
     "execution_count": 51,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Selecionando duas linhas por listas\n",
    "ex.loc[[89623142, 89623901]]"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 52,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "'exonic'"
      ]
     },
     "execution_count": 52,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Selecionando o valor de uma linha e coluna especificas\n",
    "ex.loc[89623901, \"Func.refGene\"]"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 53,
   "metadata": {
    "scrolled": true
   },
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>End</th>\n",
       "      <th>Func.refGene</th>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>Start</th>\n",
       "      <th></th>\n",
       "      <th></th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>89623142</th>\n",
       "      <td>89623142</td>\n",
       "      <td>UTR5</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>89623861</th>\n",
       "      <td>89623861</td>\n",
       "      <td>splicing</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>89623901</th>\n",
       "      <td>89623901</td>\n",
       "      <td>exonic</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "</div>"
      ],
      "text/plain": [
       "               End Func.refGene\n",
       "Start                          \n",
       "89623142  89623142         UTR5\n",
       "89623861  89623861     splicing\n",
       "89623901  89623901       exonic"
      ]
     },
     "execution_count": 53,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Selecionando linhas de uma coluna via slicing\n",
    "ex.loc[89623142:89623901, [\"End\",\"Func.refGene\"]]"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Para saber quais os valores estao no seu índice use o método `index`"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 54,
   "metadata": {
    "scrolled": true
   },
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "RangeIndex(start=0, stop=92, step=1)\n",
      "Int64Index([89622938, 89623142, 89623861, 89623901, 89654121, 89654190,\n",
      "            89711576, 89718057, 89720548, 89720634, 89720907, 89721094,\n",
      "            89725294, 89725582, 32889968, 32899567, 32899878, 32905220,\n",
      "            32905265, 32907536, 32911888, 32912299, 32913055, 32915005,\n",
      "            32915411, 32915524, 32919637, 32920016, 32920618, 32920844,\n",
      "            32929058, 32929232, 32929387, 32930373, 32936646, 32950658,\n",
      "            32951111, 32951115, 32953388, 32954561, 32954692, 32954741,\n",
      "            32968591, 32968607, 32970736, 32971425, 32973012, 23615043,\n",
      "            23640160, 68770847, 68771122, 68771372, 68771418, 68771547,\n",
      "            68772681, 68820717, 68847691, 68847692, 68847694, 68847718,\n",
      "            68847719, 68847726, 68847727, 68855852, 68856474, 68857141,\n",
      "            68857792, 68867612,  7576265,  7576443,  7577679,  7578115,\n",
      "             7578645,  7578712,  7578712,  7578837,  7579472,  7579644,\n",
      "             7579801,  7590593,  7590601,  7590607, 41196822, 41197940,\n",
      "            41247621, 41256075, 41256088, 41258136,  1206797,  1219444,\n",
      "             1219451,  1221418],\n",
      "           dtype='int64', name='Start')\n"
     ]
    }
   ],
   "source": [
    "print(multianno_df.index)\n",
    "print(ex.index)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 55,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Start</th>\n",
       "      <th>Chr</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>Func.refGene</th>\n",
       "      <th>Gene.refGene</th>\n",
       "      <th>GeneDetail.refGene</th>\n",
       "      <th>ExonicFunc.refGene</th>\n",
       "      <th>AAChange.refGene</th>\n",
       "      <th>...</th>\n",
       "      <th>VCF_CHR</th>\n",
       "      <th>VCF_POS</th>\n",
       "      <th>VCF_ID</th>\n",
       "      <th>VCF_REF</th>\n",
       "      <th>VCF_ALT</th>\n",
       "      <th>VCF_QUAL_2</th>\n",
       "      <th>VCF_FILTER</th>\n",
       "      <th>VCF_INFO</th>\n",
       "      <th>VCF_FORMAT</th>\n",
       "      <th>VCF_SAMPLE_NAME</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>89622938</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>NM_001126049:c.-694T&gt;G</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>290.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1266,552:1820:99:319,0,12285</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>89623142</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>NM_001126049:c.-898G&gt;A</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>7963.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:306,267:574:99:7992,0,9864</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>89623861</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623861</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>splicing</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304717:exon1:c.154+1T&gt;-;NM_001304717:exo...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623860</td>\n",
       "      <td>rs71022512</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>234.80</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,6:7:18:272,18,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>89623901</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>exonic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>nonsynonymous SNV</td>\n",
       "      <td>PTEN:NM_001304717:exon2:c.194G&gt;C:p.C65S</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>rs2943772</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>440.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,14:14:36:469,36,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>89654121</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>rs139651072</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>216.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:10,8:18:99:245,0,289</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>...</th>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>87</th>\n",
       "      <td>41258136</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41258136</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41258135</td>\n",
       "      <td>.</td>\n",
       "      <td>TA</td>\n",
       "      <td>T</td>\n",
       "      <td>203.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-1.019;DP=69;Exc...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:36,24:69:99:241,0,424</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>88</th>\n",
       "      <td>1206797</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1206797</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>STK11</td>\n",
       "      <td>NM_000455:c.-116del-</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1206796</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>2222.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=1.767;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:604,172:776:99:1:0.5:2260,0,15656:245,359,...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>89</th>\n",
       "      <td>1219444</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1219444</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>STK11</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1219444</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>672.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.307;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.8...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:39,88:127:99:701,0,458</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>90</th>\n",
       "      <td>1219451</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1219451</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>STK11</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1219451</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>213.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.654;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.6...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:117,62:179:99:242,0,1041</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>91</th>\n",
       "      <td>1221418</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>STK11</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418</td>\n",
       "      <td>.</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>25.78</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.417;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.9...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:108,151:263:54:54,0,1020</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>92 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "       Start    Chr       End Ref Alt Func.refGene Gene.refGene  \\\n",
       "0   89622938  chr10  89622938   A   C         UTR5         KLLN   \n",
       "1   89623142  chr10  89623142   C   T         UTR5         KLLN   \n",
       "2   89623861  chr10  89623861   T   -     splicing         PTEN   \n",
       "3   89623901  chr10  89623901   G   C       exonic         PTEN   \n",
       "4   89654121  chr10  89654121   T   C     intronic         PTEN   \n",
       "..       ...    ...       ...  ..  ..          ...          ...   \n",
       "87  41258136  chr17  41258136   A   -     intronic        BRCA1   \n",
       "88   1206797  chr19   1206797   T   -         UTR5        STK11   \n",
       "89   1219444  chr19   1219444   C   G     intronic        STK11   \n",
       "90   1219451  chr19   1219451   C   G     intronic        STK11   \n",
       "91   1221418  chr19   1221418   T   G     intronic        STK11   \n",
       "\n",
       "                                   GeneDetail.refGene ExonicFunc.refGene  \\\n",
       "0                              NM_001126049:c.-694T>G                  .   \n",
       "1                              NM_001126049:c.-898G>A                  .   \n",
       "2   NM_001304717:exon1:c.154+1T>-;NM_001304717:exo...                  .   \n",
       "3                                                   .  nonsynonymous SNV   \n",
       "4                                                   .                  .   \n",
       "..                                                ...                ...   \n",
       "87                                                  .                  .   \n",
       "88                               NM_000455:c.-116del-                  .   \n",
       "89                                                  .                  .   \n",
       "90                                                  .                  .   \n",
       "91                                                  .                  .   \n",
       "\n",
       "                           AAChange.refGene  ... VCF_CHR   VCF_POS  \\\n",
       "0                                         .  ...   chr10  89622938   \n",
       "1                                         .  ...   chr10  89623142   \n",
       "2                                         .  ...   chr10  89623860   \n",
       "3   PTEN:NM_001304717:exon2:c.194G>C:p.C65S  ...   chr10  89623901   \n",
       "4                                         .  ...   chr10  89654121   \n",
       "..                                      ...  ...     ...       ...   \n",
       "87                                        .  ...   chr17  41258135   \n",
       "88                                        .  ...   chr19   1206796   \n",
       "89                                        .  ...   chr19   1219444   \n",
       "90                                        .  ...   chr19   1219451   \n",
       "91                                        .  ...   chr19   1221418   \n",
       "\n",
       "         VCF_ID VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "0             .       A       C     290.77          .   \n",
       "1             .       C       T    7963.77          .   \n",
       "2    rs71022512      CT       C     234.80          .   \n",
       "3     rs2943772       G       C     440.77          .   \n",
       "4   rs139651072       T       C     216.77          .   \n",
       "..          ...     ...     ...        ...        ...   \n",
       "87            .      TA       T     203.73          .   \n",
       "88            .      CT       C    2222.73          .   \n",
       "89            .       C       G     672.77          .   \n",
       "90            .       C       G     213.77          .   \n",
       "91            .       T       G      25.78          .   \n",
       "\n",
       "                                             VCF_INFO  \\\n",
       "0   ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....   \n",
       "1   ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...   \n",
       "2   AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...   \n",
       "3   ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...   \n",
       "4   ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...   \n",
       "..                                                ...   \n",
       "87  AC=1;AF=0.5;AN=2;BaseQRankSum=-1.019;DP=69;Exc...   \n",
       "88  AC=1;AF=0.5;AN=2;BaseQRankSum=1.767;ClippingRa...   \n",
       "89  ABHet=0.307;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.8...   \n",
       "90  ABHet=0.654;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.6...   \n",
       "91  ABHet=0.417;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.9...   \n",
       "\n",
       "                             VCF_FORMAT  \\\n",
       "0                        GT:AD:DP:GQ:PL   \n",
       "1                        GT:AD:DP:GQ:PL   \n",
       "2                        GT:AD:DP:GQ:PL   \n",
       "3                        GT:AD:DP:GQ:PL   \n",
       "4                        GT:AD:DP:GQ:PL   \n",
       "..                                  ...   \n",
       "87                       GT:AD:DP:GQ:PL   \n",
       "88  GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "89                       GT:AD:DP:GQ:PL   \n",
       "90                       GT:AD:DP:GQ:PL   \n",
       "91                       GT:AD:DP:GQ:PL   \n",
       "\n",
       "                                      VCF_SAMPLE_NAME  \n",
       "0                    0/1:1266,552:1820:99:319,0,12285  \n",
       "1                      0/1:306,267:574:99:7992,0,9864  \n",
       "2                               1/1:0,6:7:18:272,18,0  \n",
       "3                             1/1:0,14:14:36:469,36,0  \n",
       "4                            0/1:10,8:18:99:245,0,289  \n",
       "..                                                ...  \n",
       "87                          0/1:36,24:69:99:241,0,424  \n",
       "88  0/1:604,172:776:99:1:0.5:2260,0,15656:245,359,...  \n",
       "89                         0/1:39,88:127:99:701,0,458  \n",
       "90                       0/1:117,62:179:99:242,0,1041  \n",
       "91                       0/1:108,151:263:54:54,0,1020  \n",
       "\n",
       "[92 rows x 183 columns]"
      ]
     },
     "execution_count": 55,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Resetando o index\n",
    "ex.reset_index()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 56,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>Func.refGene</th>\n",
       "      <th>Gene.refGene</th>\n",
       "      <th>GeneDetail.refGene</th>\n",
       "      <th>ExonicFunc.refGene</th>\n",
       "      <th>AAChange.refGene</th>\n",
       "      <th>...</th>\n",
       "      <th>VCF_CHR</th>\n",
       "      <th>VCF_POS</th>\n",
       "      <th>VCF_ID</th>\n",
       "      <th>VCF_REF</th>\n",
       "      <th>VCF_ALT</th>\n",
       "      <th>VCF_QUAL_2</th>\n",
       "      <th>VCF_FILTER</th>\n",
       "      <th>VCF_INFO</th>\n",
       "      <th>VCF_FORMAT</th>\n",
       "      <th>VCF_SAMPLE_NAME</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7579644</td>\n",
       "      <td>7579659</td>\n",
       "      <td>CCCCAGCCCTCCAGGT</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7579643</td>\n",
       "      <td>rs146534833</td>\n",
       "      <td>CCCCCAGCCCTCCAGGT</td>\n",
       "      <td>C</td>\n",
       "      <td>122210.00</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=2;AF=1;AN=2;DB;DP=1405;ExcessHet=3.0103;FS=...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,924:1405:99:122247,2780,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7579801</td>\n",
       "      <td>7579801</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>TP53</td>\n",
       "      <td>NM_001126118:c.-232C&gt;G</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7579801</td>\n",
       "      <td>rs1642785</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>9509.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=0.651;AC=2;AF=1;AN=2;BaseQRankSum=16.02;...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:203,378:582:99:9538,522,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7590593</td>\n",
       "      <td>7590593</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53;WRAP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7590593</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>87.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.749;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.3...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:188,63:251:99:116,0,652</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7590601</td>\n",
       "      <td>7590601</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53;WRAP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7590601</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>339.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.585;AC=1;AF=0.5;AN=2;BaseQRankSum=-2.0...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:172,122:294:99:368,0,1128</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7590607</td>\n",
       "      <td>7590607</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53;WRAP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7590607</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>41.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.704;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.0...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:226,95:321:70:70,0,2914</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>5</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41196822</td>\n",
       "      <td>41196824</td>\n",
       "      <td>TTT</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>NM_007300:c.*873_*871delAAA;NM_007297:c.*873_*...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41196821</td>\n",
       "      <td>.</td>\n",
       "      <td>CTTT</td>\n",
       "      <td>C</td>\n",
       "      <td>84.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.689;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:109,19:128:99:1:0.5:122,0,3145:69,40,12,7:...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>6</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41197940</td>\n",
       "      <td>41197940</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41197939</td>\n",
       "      <td>.</td>\n",
       "      <td>AT</td>\n",
       "      <td>A</td>\n",
       "      <td>1932.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=3.135;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:60,107:167:99:1:0.5:1970,0,654:2,58,33,74:...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>7</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41247621</td>\n",
       "      <td>41247621</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41247620</td>\n",
       "      <td>.</td>\n",
       "      <td>CA</td>\n",
       "      <td>C</td>\n",
       "      <td>197.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.097;DP=38;Exc...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:24,13:38:99:235,0,434</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>8</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41256075</td>\n",
       "      <td>41256075</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41256074</td>\n",
       "      <td>.</td>\n",
       "      <td>CA</td>\n",
       "      <td>C</td>\n",
       "      <td>932.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.364;DP=201;Exc...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:128,67:201:99:970,0,2530</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>9</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41256088</td>\n",
       "      <td>41256088</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41256087</td>\n",
       "      <td>.</td>\n",
       "      <td>GA</td>\n",
       "      <td>G</td>\n",
       "      <td>357.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.38;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:83,28:111:99:1:0.5:395,0,2036:83,0,28,0:70...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>10</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41258136</td>\n",
       "      <td>41258136</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41258135</td>\n",
       "      <td>.</td>\n",
       "      <td>TA</td>\n",
       "      <td>T</td>\n",
       "      <td>203.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-1.019;DP=69;Exc...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:36,24:69:99:241,0,424</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>11</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1206797</td>\n",
       "      <td>1206797</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>STK11</td>\n",
       "      <td>NM_000455:c.-116del-</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1206796</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>2222.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=1.767;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:604,172:776:99:1:0.5:2260,0,15656:245,359,...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>12</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1219444</td>\n",
       "      <td>1219444</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>STK11</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1219444</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>672.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.307;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.8...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:39,88:127:99:701,0,458</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>13</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1219451</td>\n",
       "      <td>1219451</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>STK11</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1219451</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>213.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.654;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.6...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:117,62:179:99:242,0,1041</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>14</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418</td>\n",
       "      <td>1221418</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>STK11</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418</td>\n",
       "      <td>.</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>25.78</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.417;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.9...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:108,151:263:54:54,0,1020</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>15 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr     Start       End               Ref Alt Func.refGene Gene.refGene  \\\n",
       "0   chr17   7579644   7579659  CCCCAGCCCTCCAGGT   -     intronic         TP53   \n",
       "1   chr17   7579801   7579801                 G   C         UTR5         TP53   \n",
       "2   chr17   7590593   7590593                 A   C     intronic  TP53;WRAP53   \n",
       "3   chr17   7590601   7590601                 A   C     intronic  TP53;WRAP53   \n",
       "4   chr17   7590607   7590607                 A   C     intronic  TP53;WRAP53   \n",
       "5   chr17  41196822  41196824               TTT   -         UTR3        BRCA1   \n",
       "6   chr17  41197940  41197940                 T   -     intronic        BRCA1   \n",
       "7   chr17  41247621  41247621                 A   -     intronic        BRCA1   \n",
       "8   chr17  41256075  41256075                 A   -     intronic        BRCA1   \n",
       "9   chr17  41256088  41256088                 A   -     intronic        BRCA1   \n",
       "10  chr17  41258136  41258136                 A   -     intronic        BRCA1   \n",
       "11  chr19   1206797   1206797                 T   -         UTR5        STK11   \n",
       "12  chr19   1219444   1219444                 C   G     intronic        STK11   \n",
       "13  chr19   1219451   1219451                 C   G     intronic        STK11   \n",
       "14  chr19   1221418   1221418                 T   G     intronic        STK11   \n",
       "\n",
       "                                   GeneDetail.refGene ExonicFunc.refGene  \\\n",
       "0                                                   .                  .   \n",
       "1                              NM_001126118:c.-232C>G                  .   \n",
       "2                                                   .                  .   \n",
       "3                                                   .                  .   \n",
       "4                                                   .                  .   \n",
       "5   NM_007300:c.*873_*871delAAA;NM_007297:c.*873_*...                  .   \n",
       "6                                                   .                  .   \n",
       "7                                                   .                  .   \n",
       "8                                                   .                  .   \n",
       "9                                                   .                  .   \n",
       "10                                                  .                  .   \n",
       "11                               NM_000455:c.-116del-                  .   \n",
       "12                                                  .                  .   \n",
       "13                                                  .                  .   \n",
       "14                                                  .                  .   \n",
       "\n",
       "   AAChange.refGene  ... VCF_CHR   VCF_POS       VCF_ID            VCF_REF  \\\n",
       "0                 .  ...   chr17   7579643  rs146534833  CCCCCAGCCCTCCAGGT   \n",
       "1                 .  ...   chr17   7579801    rs1642785                  G   \n",
       "2                 .  ...   chr17   7590593            .                  A   \n",
       "3                 .  ...   chr17   7590601            .                  A   \n",
       "4                 .  ...   chr17   7590607            .                  A   \n",
       "5                 .  ...   chr17  41196821            .               CTTT   \n",
       "6                 .  ...   chr17  41197939            .                 AT   \n",
       "7                 .  ...   chr17  41247620            .                 CA   \n",
       "8                 .  ...   chr17  41256074            .                 CA   \n",
       "9                 .  ...   chr17  41256087            .                 GA   \n",
       "10                .  ...   chr17  41258135            .                 TA   \n",
       "11                .  ...   chr19   1206796            .                 CT   \n",
       "12                .  ...   chr19   1219444            .                  C   \n",
       "13                .  ...   chr19   1219451            .                  C   \n",
       "14                .  ...   chr19   1221418            .                  T   \n",
       "\n",
       "   VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "0        C  122210.00          .   \n",
       "1        C    9509.77          .   \n",
       "2        C      87.77          .   \n",
       "3        C     339.77          .   \n",
       "4        C      41.77          .   \n",
       "5        C      84.73          .   \n",
       "6        A    1932.73          .   \n",
       "7        C     197.73          .   \n",
       "8        C     932.73          .   \n",
       "9        G     357.73          .   \n",
       "10       T     203.73          .   \n",
       "11       C    2222.73          .   \n",
       "12       G     672.77          .   \n",
       "13       G     213.77          .   \n",
       "14       G      25.78          .   \n",
       "\n",
       "                                             VCF_INFO  \\\n",
       "0   AC=2;AF=1;AN=2;DB;DP=1405;ExcessHet=3.0103;FS=...   \n",
       "1   ABHom=0.651;AC=2;AF=1;AN=2;BaseQRankSum=16.02;...   \n",
       "2   ABHet=0.749;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.3...   \n",
       "3   ABHet=0.585;AC=1;AF=0.5;AN=2;BaseQRankSum=-2.0...   \n",
       "4   ABHet=0.704;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.0...   \n",
       "5   AC=1;AF=0.5;AN=2;BaseQRankSum=0.689;ClippingRa...   \n",
       "6   AC=1;AF=0.5;AN=2;BaseQRankSum=3.135;ClippingRa...   \n",
       "7   AC=1;AF=0.5;AN=2;BaseQRankSum=-0.097;DP=38;Exc...   \n",
       "8   AC=1;AF=0.5;AN=2;BaseQRankSum=0.364;DP=201;Exc...   \n",
       "9   AC=1;AF=0.5;AN=2;BaseQRankSum=-0.38;ClippingRa...   \n",
       "10  AC=1;AF=0.5;AN=2;BaseQRankSum=-1.019;DP=69;Exc...   \n",
       "11  AC=1;AF=0.5;AN=2;BaseQRankSum=1.767;ClippingRa...   \n",
       "12  ABHet=0.307;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.8...   \n",
       "13  ABHet=0.654;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.6...   \n",
       "14  ABHet=0.417;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.9...   \n",
       "\n",
       "                             VCF_FORMAT  \\\n",
       "0                        GT:AD:DP:GQ:PL   \n",
       "1                        GT:AD:DP:GQ:PL   \n",
       "2                        GT:AD:DP:GQ:PL   \n",
       "3                        GT:AD:DP:GQ:PL   \n",
       "4                        GT:AD:DP:GQ:PL   \n",
       "5   GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "6   GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "7                        GT:AD:DP:GQ:PL   \n",
       "8                        GT:AD:DP:GQ:PL   \n",
       "9   GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "10                       GT:AD:DP:GQ:PL   \n",
       "11  GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "12                       GT:AD:DP:GQ:PL   \n",
       "13                       GT:AD:DP:GQ:PL   \n",
       "14                       GT:AD:DP:GQ:PL   \n",
       "\n",
       "                                      VCF_SAMPLE_NAME  \n",
       "0                     1/1:0,924:1405:99:122247,2780,0  \n",
       "1                       1/1:203,378:582:99:9538,522,0  \n",
       "2                         0/1:188,63:251:99:116,0,652  \n",
       "3                       0/1:172,122:294:99:368,0,1128  \n",
       "4                         0/1:226,95:321:70:70,0,2914  \n",
       "5   0/1:109,19:128:99:1:0.5:122,0,3145:69,40,12,7:...  \n",
       "6   0/1:60,107:167:99:1:0.5:1970,0,654:2,58,33,74:...  \n",
       "7                           0/1:24,13:38:99:235,0,434  \n",
       "8                        0/1:128,67:201:99:970,0,2530  \n",
       "9   0/1:83,28:111:99:1:0.5:395,0,2036:83,0,28,0:70...  \n",
       "10                          0/1:36,24:69:99:241,0,424  \n",
       "11  0/1:604,172:776:99:1:0.5:2260,0,15656:245,359,...  \n",
       "12                         0/1:39,88:127:99:701,0,458  \n",
       "13                       0/1:117,62:179:99:242,0,1041  \n",
       "14                       0/1:108,151:263:54:54,0,1020  \n",
       "\n",
       "[15 rows x 183 columns]"
      ]
     },
     "execution_count": 56,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "df2.reset_index(drop=True)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# Filtrando um `DataFrame`\n",
    "\n",
    "* É possível filtrar um `DataFrame` maior em um `DataFrame` menor. Por exemplo, se eu quisesse visualizar apenas as variantes no cromossomo 10:"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 57,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "0      True\n",
       "1      True\n",
       "2      True\n",
       "3      True\n",
       "4      True\n",
       "      ...  \n",
       "87    False\n",
       "88    False\n",
       "89    False\n",
       "90    False\n",
       "91    False\n",
       "Name: Chr, Length: 92, dtype: bool"
      ]
     },
     "execution_count": 57,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "str_filter = multianno_df['Chr'] == \"chr10\"\n",
    "str_filter"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 58,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>Func.refGene</th>\n",
       "      <th>Gene.refGene</th>\n",
       "      <th>GeneDetail.refGene</th>\n",
       "      <th>ExonicFunc.refGene</th>\n",
       "      <th>AAChange.refGene</th>\n",
       "      <th>...</th>\n",
       "      <th>VCF_CHR</th>\n",
       "      <th>VCF_POS</th>\n",
       "      <th>VCF_ID</th>\n",
       "      <th>VCF_REF</th>\n",
       "      <th>VCF_ALT</th>\n",
       "      <th>VCF_QUAL_2</th>\n",
       "      <th>VCF_FILTER</th>\n",
       "      <th>VCF_INFO</th>\n",
       "      <th>VCF_FORMAT</th>\n",
       "      <th>VCF_SAMPLE_NAME</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>89622938</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>NM_001126049:c.-694T&gt;G</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>290.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1266,552:1820:99:319,0,12285</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>89623142</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>NM_001126049:c.-898G&gt;A</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>7963.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:306,267:574:99:7992,0,9864</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623861</td>\n",
       "      <td>89623861</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>splicing</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304717:exon1:c.154+1T&gt;-;NM_001304717:exo...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623860</td>\n",
       "      <td>rs71022512</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>234.80</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,6:7:18:272,18,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>89623901</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>exonic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>nonsynonymous SNV</td>\n",
       "      <td>PTEN:NM_001304717:exon2:c.194G&gt;C:p.C65S</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>rs2943772</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>440.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,14:14:36:469,36,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>89654121</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>rs139651072</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>216.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:10,8:18:99:245,0,289</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>5</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190</td>\n",
       "      <td>89654190</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190</td>\n",
       "      <td>rs181780682</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>29.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.714;AC=1;AF=0.5;AN=2;BaseQRankSum=0.36...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:5,2:7:58:58,0,172</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>6</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89711576</td>\n",
       "      <td>89711576</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89711576</td>\n",
       "      <td>rs141005791</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>96.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.21...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:5,4:9:99:125,0,133</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>7</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89718057</td>\n",
       "      <td>89718057</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89718057</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>759.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.324;AC=1;AF=0.5;AN=2;BaseQRankSum=0.83...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:11,23:34:99:788,0,283</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>8</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720548</td>\n",
       "      <td>89720548</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720548</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>1491.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.467;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.5...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:42,48:90:99:1520,0,1330</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>9</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720634</td>\n",
       "      <td>89720634</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720633</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>1805.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.601;DP=689;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:443,192:689:99:1843,0,6330</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>10</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720907</td>\n",
       "      <td>89720907</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720907</td>\n",
       "      <td>rs555895</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>10070.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.555;AC=1;AF=0.5;AN=2;BaseQRankSum=6.08...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:397,318:717:99:10099,0,11475</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>11</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89721094</td>\n",
       "      <td>89721094</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89721094</td>\n",
       "      <td>rs2736627</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>3289.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=92;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,92:92:99:3318,259,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>12</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725294</td>\n",
       "      <td>89725294</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304718:c.*65delT;NM_001304717:c.*65delT;...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89725293</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>9448.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.417;DP=2406;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1610,625:2406:99:9486,0,34365</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>13</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582</td>\n",
       "      <td>89725582</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304718:c.*353C&gt;T;NM_001304717:c.*353C&gt;T;...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582</td>\n",
       "      <td>rs181234898</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>201.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.526;AC=1;AF=0.5;AN=2;BaseQRankSum=0.31...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:10,9:19:99:230,0,266</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>14 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr     Start       End Ref Alt Func.refGene Gene.refGene  \\\n",
       "0   chr10  89622938  89622938   A   C         UTR5         KLLN   \n",
       "1   chr10  89623142  89623142   C   T         UTR5         KLLN   \n",
       "2   chr10  89623861  89623861   T   -     splicing         PTEN   \n",
       "3   chr10  89623901  89623901   G   C       exonic         PTEN   \n",
       "4   chr10  89654121  89654121   T   C     intronic         PTEN   \n",
       "5   chr10  89654190  89654190   G   A     intronic         PTEN   \n",
       "6   chr10  89711576  89711576   C   T     intronic         PTEN   \n",
       "7   chr10  89718057  89718057   A   G     intronic         PTEN   \n",
       "8   chr10  89720548  89720548   A   G     intronic         PTEN   \n",
       "9   chr10  89720634  89720634   T   -     intronic         PTEN   \n",
       "10  chr10  89720907  89720907   T   G     intronic         PTEN   \n",
       "11  chr10  89721094  89721094   C   T     intronic         PTEN   \n",
       "12  chr10  89725294  89725294   T   -         UTR3         PTEN   \n",
       "13  chr10  89725582  89725582   C   T         UTR3         PTEN   \n",
       "\n",
       "                                   GeneDetail.refGene ExonicFunc.refGene  \\\n",
       "0                              NM_001126049:c.-694T>G                  .   \n",
       "1                              NM_001126049:c.-898G>A                  .   \n",
       "2   NM_001304717:exon1:c.154+1T>-;NM_001304717:exo...                  .   \n",
       "3                                                   .  nonsynonymous SNV   \n",
       "4                                                   .                  .   \n",
       "5                                                   .                  .   \n",
       "6                                                   .                  .   \n",
       "7                                                   .                  .   \n",
       "8                                                   .                  .   \n",
       "9                                                   .                  .   \n",
       "10                                                  .                  .   \n",
       "11                                                  .                  .   \n",
       "12  NM_001304718:c.*65delT;NM_001304717:c.*65delT;...                  .   \n",
       "13  NM_001304718:c.*353C>T;NM_001304717:c.*353C>T;...                  .   \n",
       "\n",
       "                           AAChange.refGene  ... VCF_CHR   VCF_POS  \\\n",
       "0                                         .  ...   chr10  89622938   \n",
       "1                                         .  ...   chr10  89623142   \n",
       "2                                         .  ...   chr10  89623860   \n",
       "3   PTEN:NM_001304717:exon2:c.194G>C:p.C65S  ...   chr10  89623901   \n",
       "4                                         .  ...   chr10  89654121   \n",
       "5                                         .  ...   chr10  89654190   \n",
       "6                                         .  ...   chr10  89711576   \n",
       "7                                         .  ...   chr10  89718057   \n",
       "8                                         .  ...   chr10  89720548   \n",
       "9                                         .  ...   chr10  89720633   \n",
       "10                                        .  ...   chr10  89720907   \n",
       "11                                        .  ...   chr10  89721094   \n",
       "12                                        .  ...   chr10  89725293   \n",
       "13                                        .  ...   chr10  89725582   \n",
       "\n",
       "         VCF_ID VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "0             .       A       C     290.77          .   \n",
       "1             .       C       T    7963.77          .   \n",
       "2    rs71022512      CT       C     234.80          .   \n",
       "3     rs2943772       G       C     440.77          .   \n",
       "4   rs139651072       T       C     216.77          .   \n",
       "5   rs181780682       G       A      29.77          .   \n",
       "6   rs141005791       C       T      96.77          .   \n",
       "7             .       A       G     759.77          .   \n",
       "8             .       A       G    1491.77          .   \n",
       "9             .      CT       C    1805.73          .   \n",
       "10     rs555895       T       G   10070.80          .   \n",
       "11    rs2736627       C       T    3289.77          .   \n",
       "12            .      CT       C    9448.73          .   \n",
       "13  rs181234898       C       T     201.77          .   \n",
       "\n",
       "                                             VCF_INFO      VCF_FORMAT  \\\n",
       "0   ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....  GT:AD:DP:GQ:PL   \n",
       "1   ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...  GT:AD:DP:GQ:PL   \n",
       "2   AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...  GT:AD:DP:GQ:PL   \n",
       "3   ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...  GT:AD:DP:GQ:PL   \n",
       "4   ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...  GT:AD:DP:GQ:PL   \n",
       "5   ABHet=0.714;AC=1;AF=0.5;AN=2;BaseQRankSum=0.36...  GT:AD:DP:GQ:PL   \n",
       "6   ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.21...  GT:AD:DP:GQ:PL   \n",
       "7   ABHet=0.324;AC=1;AF=0.5;AN=2;BaseQRankSum=0.83...  GT:AD:DP:GQ:PL   \n",
       "8   ABHet=0.467;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.5...  GT:AD:DP:GQ:PL   \n",
       "9   AC=1;AF=0.5;AN=2;BaseQRankSum=-0.601;DP=689;Ex...  GT:AD:DP:GQ:PL   \n",
       "10  ABHet=0.555;AC=1;AF=0.5;AN=2;BaseQRankSum=6.08...  GT:AD:DP:GQ:PL   \n",
       "11  ABHom=1;AC=2;AF=1;AN=2;DB;DP=92;Dels=0;ExcessH...  GT:AD:DP:GQ:PL   \n",
       "12  AC=1;AF=0.5;AN=2;BaseQRankSum=0.417;DP=2406;Ex...  GT:AD:DP:GQ:PL   \n",
       "13  ABHet=0.526;AC=1;AF=0.5;AN=2;BaseQRankSum=0.31...  GT:AD:DP:GQ:PL   \n",
       "\n",
       "                      VCF_SAMPLE_NAME  \n",
       "0    0/1:1266,552:1820:99:319,0,12285  \n",
       "1      0/1:306,267:574:99:7992,0,9864  \n",
       "2               1/1:0,6:7:18:272,18,0  \n",
       "3             1/1:0,14:14:36:469,36,0  \n",
       "4            0/1:10,8:18:99:245,0,289  \n",
       "5               0/1:5,2:7:58:58,0,172  \n",
       "6              0/1:5,4:9:99:125,0,133  \n",
       "7           0/1:11,23:34:99:788,0,283  \n",
       "8         0/1:42,48:90:99:1520,0,1330  \n",
       "9      0/1:443,192:689:99:1843,0,6330  \n",
       "10   0/1:397,318:717:99:10099,0,11475  \n",
       "11          1/1:0,92:92:99:3318,259,0  \n",
       "12  0/1:1610,625:2406:99:9486,0,34365  \n",
       "13           0/1:10,9:19:99:230,0,266  \n",
       "\n",
       "[14 rows x 183 columns]"
      ]
     },
     "execution_count": 58,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df[str_filter]"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Se por acaso eu quisesse criar um `DataFrame` com variantes extremamente raras nesse paciente?"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 59,
   "metadata": {
    "scrolled": true
   },
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
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       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>Func.refGene</th>\n",
       "      <th>Gene.refGene</th>\n",
       "      <th>GeneDetail.refGene</th>\n",
       "      <th>ExonicFunc.refGene</th>\n",
       "      <th>AAChange.refGene</th>\n",
       "      <th>...</th>\n",
       "      <th>VCF_CHR</th>\n",
       "      <th>VCF_POS</th>\n",
       "      <th>VCF_ID</th>\n",
       "      <th>VCF_REF</th>\n",
       "      <th>VCF_ALT</th>\n",
       "      <th>VCF_QUAL_2</th>\n",
       "      <th>VCF_FILTER</th>\n",
       "      <th>VCF_INFO</th>\n",
       "      <th>VCF_FORMAT</th>\n",
       "      <th>VCF_SAMPLE_NAME</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>89622938</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>NM_001126049:c.-694T&gt;G</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>290.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1266,552:1820:99:319,0,12285</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>89623142</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>KLLN</td>\n",
       "      <td>NM_001126049:c.-898G&gt;A</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>7963.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:306,267:574:99:7992,0,9864</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>89654121</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>rs139651072</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>216.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:10,8:18:99:245,0,289</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>5</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190</td>\n",
       "      <td>89654190</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190</td>\n",
       "      <td>rs181780682</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>29.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.714;AC=1;AF=0.5;AN=2;BaseQRankSum=0.36...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:5,2:7:58:58,0,172</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>6</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89711576</td>\n",
       "      <td>89711576</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89711576</td>\n",
       "      <td>rs141005791</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>96.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.21...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:5,4:9:99:125,0,133</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>7</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89718057</td>\n",
       "      <td>89718057</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89718057</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>759.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.324;AC=1;AF=0.5;AN=2;BaseQRankSum=0.83...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:11,23:34:99:788,0,283</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>8</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720548</td>\n",
       "      <td>89720548</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720548</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>1491.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.467;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.5...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:42,48:90:99:1520,0,1330</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>9</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720634</td>\n",
       "      <td>89720634</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720633</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>1805.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.601;DP=689;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:443,192:689:99:1843,0,6330</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>12</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725294</td>\n",
       "      <td>89725294</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304718:c.*65delT;NM_001304717:c.*65delT;...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89725293</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>9448.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.417;DP=2406;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1610,625:2406:99:9486,0,34365</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>13</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582</td>\n",
       "      <td>89725582</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304718:c.*353C&gt;T;NM_001304717:c.*353C&gt;T;...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582</td>\n",
       "      <td>rs181234898</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>201.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.526;AC=1;AF=0.5;AN=2;BaseQRankSum=0.31...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:10,9:19:99:230,0,266</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>17</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32905220</td>\n",
       "      <td>32905220</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32905219</td>\n",
       "      <td>.</td>\n",
       "      <td>AT</td>\n",
       "      <td>A</td>\n",
       "      <td>2225.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=4.537;DP=630;Exc...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:413,156:630:99:2263,0,8472</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>19</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32907536</td>\n",
       "      <td>32907536</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32907535</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>4200.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=3.476;DP=1016;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:669,272:1016:99:4238,0,14585</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>30</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32929058</td>\n",
       "      <td>32929059</td>\n",
       "      <td>TC</td>\n",
       "      <td>-</td>\n",
       "      <td>exonic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>frameshift deletion</td>\n",
       "      <td>BRCA2:NM_000059:exon14:c.7068_7069del:p.F2356fs</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32929057</td>\n",
       "      <td>rs80359636</td>\n",
       "      <td>TTC</td>\n",
       "      <td>T</td>\n",
       "      <td>33599.70</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=2.352;DB;DP=1613...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:853,734:1613:99:33637,0,39748</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>33</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32930373</td>\n",
       "      <td>32930373</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32930372</td>\n",
       "      <td>.</td>\n",
       "      <td>AT</td>\n",
       "      <td>A</td>\n",
       "      <td>119.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.879;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:27,10:37:99:1:0.5:157,0,642:27,0,10,0:27,0...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>40</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32954692</td>\n",
       "      <td>32954692</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32954692</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>21.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.625;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.2...</td>\n",
       "      <td>GT:AB:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:0.63:5,3:8:50:1:0.5:50,0,94:0,5,0,3:0,5,0,3</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>48</th>\n",
       "      <td>chr16</td>\n",
       "      <td>23640160</td>\n",
       "      <td>23640160</td>\n",
       "      <td>G</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PALB2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr16</td>\n",
       "      <td>23640160</td>\n",
       "      <td>.</td>\n",
       "      <td>G</td>\n",
       "      <td>T</td>\n",
       "      <td>35.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.6;AC=1;AF=0.5;AN=2;BaseQRankSum=0;DP=5...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:3,2:5:64:64,0,103</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>50</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68771122</td>\n",
       "      <td>68771122</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>upstream</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>dist=71</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr16</td>\n",
       "      <td>68771122</td>\n",
       "      <td>rs28372783</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>18862.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.507;AC=1;AF=0.5;AN=2;BaseQRankSum=11.1...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:685,666:1355:99:18891,0,17969</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>55</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68820717</td>\n",
       "      <td>68820717</td>\n",
       "      <td>C</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr16</td>\n",
       "      <td>68820717</td>\n",
       "      <td>rs146972234</td>\n",
       "      <td>C</td>\n",
       "      <td>A</td>\n",
       "      <td>680.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.587;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.4...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:37,26:63:99:709,0,1225</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>56</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68847691</td>\n",
       "      <td>68847691</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr16</td>\n",
       "      <td>68847691</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>28.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.818;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.8...</td>\n",
       "      <td>GT:AB:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:0.82:9,2:11:57:1:0.5:57,0,1646:0,9,0,2:0,9...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>57</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68847692</td>\n",
       "      <td>68847692</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr16</td>\n",
       "      <td>68847692</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>28.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.818;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...</td>\n",
       "      <td>GT:AB:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:0.82:9,2:11:57:1:0.5:57,0,1646:0,9,0,2:0,9...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>58</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68847694</td>\n",
       "      <td>68847694</td>\n",
       "      <td>G</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr16</td>\n",
       "      <td>68847694</td>\n",
       "      <td>.</td>\n",
       "      <td>G</td>\n",
       "      <td>T</td>\n",
       "      <td>28.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.818;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.8...</td>\n",
       "      <td>GT:AB:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:0.82:9,2:11:57:1:0.5:57,0,1646:0,9,0,2:0,9...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>59</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68847718</td>\n",
       "      <td>68847718</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr16</td>\n",
       "      <td>68847718</td>\n",
       "      <td>.</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>34.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.778;AC=1;AF=0.5;AN=2;BaseQRankSum=0.03...</td>\n",
       "      <td>GT:AB:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:0.78:7,2:9:63:1:0.5:63,0,1502:0,7,0,2:0,7,0,2</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>60</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68847719</td>\n",
       "      <td>68847719</td>\n",
       "      <td>C</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr16</td>\n",
       "      <td>68847719</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>A</td>\n",
       "      <td>23.79</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.8;AC=1;AF=0.5;AN=2;BaseQRankSum=0.728;...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:8,2:10:52:52,0,233</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>61</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68847726</td>\n",
       "      <td>68847726</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr16</td>\n",
       "      <td>68847726</td>\n",
       "      <td>.</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>34.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.778;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.4...</td>\n",
       "      <td>GT:AB:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:0.78:7,2:9:63:1:0.5:63,0,1502:0,7,0,2:0,7,0,2</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>62</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68847727</td>\n",
       "      <td>68847727</td>\n",
       "      <td>A</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr16</td>\n",
       "      <td>68847727</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>T</td>\n",
       "      <td>23.79</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.8;AC=1;AF=0.5;AN=2;BaseQRankSum=0.493;...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:8,2:10:52:52,0,245</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>63</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68855852</td>\n",
       "      <td>68855852</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr16</td>\n",
       "      <td>68855852</td>\n",
       "      <td>rs34582129</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>9012.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.517;AC=1;AF=0.5;AN=2;BaseQRankSum=0.26...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:312,292:606:99:9041,0,9787</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>64</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68856474</td>\n",
       "      <td>68856474</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr16</td>\n",
       "      <td>68856474</td>\n",
       "      <td>.</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>38.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.6;AC=1;AF=0.5;AN=2;BaseQRankSum=0.524;...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:3,2:5:67:67,0,67</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>66</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68857792</td>\n",
       "      <td>68857792</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr16</td>\n",
       "      <td>68857792</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>22.79</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.75;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.67...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:6,2:8:51:51,0,209</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>67</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68867612</td>\n",
       "      <td>68867612</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>CDH1</td>\n",
       "      <td>NM_004360:c.*210delT;NM_001317186:c.*210delT;N...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr16</td>\n",
       "      <td>68867611</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>986.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=1.16;DP=270;Exce...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:161,71:270:99:1024,0,3177</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>68</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7576265</td>\n",
       "      <td>7576265</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7576264</td>\n",
       "      <td>.</td>\n",
       "      <td>TA</td>\n",
       "      <td>T</td>\n",
       "      <td>105.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.354;DP=29;Exce...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:20,9:29:99:143,0,380</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>69</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7576443</td>\n",
       "      <td>7576443</td>\n",
       "      <td>-</td>\n",
       "      <td>AA</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7576443</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>CAA</td>\n",
       "      <td>605.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-1.367;ClippingR...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:11,24:35:99:1:0.5:566,0,111:11,0,24,0:10,0...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>73</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7578712</td>\n",
       "      <td>7578715</td>\n",
       "      <td>TTTT</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>TP53</td>\n",
       "      <td>NM_001276697:c.-260_-263delAAAA;NM_001276698:c...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7578711</td>\n",
       "      <td>.</td>\n",
       "      <td>CTTTT</td>\n",
       "      <td>C</td>\n",
       "      <td>78271.70</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.215;DP=2426;E...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/.:152,1138:2426:99:78309,15975,16093</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>79</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7590593</td>\n",
       "      <td>7590593</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53;WRAP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7590593</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>87.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.749;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.3...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:188,63:251:99:116,0,652</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>80</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7590601</td>\n",
       "      <td>7590601</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53;WRAP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7590601</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>339.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.585;AC=1;AF=0.5;AN=2;BaseQRankSum=-2.0...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:172,122:294:99:368,0,1128</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>81</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7590607</td>\n",
       "      <td>7590607</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53;WRAP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7590607</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>41.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.704;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.0...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:226,95:321:70:70,0,2914</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>82</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41196822</td>\n",
       "      <td>41196824</td>\n",
       "      <td>TTT</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>NM_007300:c.*873_*871delAAA;NM_007297:c.*873_*...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41196821</td>\n",
       "      <td>.</td>\n",
       "      <td>CTTT</td>\n",
       "      <td>C</td>\n",
       "      <td>84.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.689;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:109,19:128:99:1:0.5:122,0,3145:69,40,12,7:...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>84</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41247621</td>\n",
       "      <td>41247621</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41247620</td>\n",
       "      <td>.</td>\n",
       "      <td>CA</td>\n",
       "      <td>C</td>\n",
       "      <td>197.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.097;DP=38;Exc...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:24,13:38:99:235,0,434</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>86</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41256088</td>\n",
       "      <td>41256088</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41256087</td>\n",
       "      <td>.</td>\n",
       "      <td>GA</td>\n",
       "      <td>G</td>\n",
       "      <td>357.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.38;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:83,28:111:99:1:0.5:395,0,2036:83,0,28,0:70...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>87</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41258136</td>\n",
       "      <td>41258136</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41258135</td>\n",
       "      <td>.</td>\n",
       "      <td>TA</td>\n",
       "      <td>T</td>\n",
       "      <td>203.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-1.019;DP=69;Exc...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:36,24:69:99:241,0,424</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>88</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1206797</td>\n",
       "      <td>1206797</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>STK11</td>\n",
       "      <td>NM_000455:c.-116del-</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1206796</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>2222.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=1.767;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:604,172:776:99:1:0.5:2260,0,15656:245,359,...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>89</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1219444</td>\n",
       "      <td>1219444</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>STK11</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1219444</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>672.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.307;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.8...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:39,88:127:99:701,0,458</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>90</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1219451</td>\n",
       "      <td>1219451</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>STK11</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr19</td>\n",
       "      <td>1219451</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>G</td>\n",
       "      <td>213.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.654;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.6...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:117,62:179:99:242,0,1041</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>42 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr     Start       End   Ref Alt Func.refGene Gene.refGene  \\\n",
       "0   chr10  89622938  89622938     A   C         UTR5         KLLN   \n",
       "1   chr10  89623142  89623142     C   T         UTR5         KLLN   \n",
       "4   chr10  89654121  89654121     T   C     intronic         PTEN   \n",
       "5   chr10  89654190  89654190     G   A     intronic         PTEN   \n",
       "6   chr10  89711576  89711576     C   T     intronic         PTEN   \n",
       "7   chr10  89718057  89718057     A   G     intronic         PTEN   \n",
       "8   chr10  89720548  89720548     A   G     intronic         PTEN   \n",
       "9   chr10  89720634  89720634     T   -     intronic         PTEN   \n",
       "12  chr10  89725294  89725294     T   -         UTR3         PTEN   \n",
       "13  chr10  89725582  89725582     C   T         UTR3         PTEN   \n",
       "17  chr13  32905220  32905220     T   -     intronic        BRCA2   \n",
       "19  chr13  32907536  32907536     T   -     intronic        BRCA2   \n",
       "30  chr13  32929058  32929059    TC   -       exonic        BRCA2   \n",
       "33  chr13  32930373  32930373     T   -     intronic        BRCA2   \n",
       "40  chr13  32954692  32954692     C   T     intronic        BRCA2   \n",
       "48  chr16  23640160  23640160     G   T     intronic        PALB2   \n",
       "50  chr16  68771122  68771122     A   C     upstream         CDH1   \n",
       "55  chr16  68820717  68820717     C   A     intronic         CDH1   \n",
       "56  chr16  68847691  68847691     C   T     intronic         CDH1   \n",
       "57  chr16  68847692  68847692     A   G     intronic         CDH1   \n",
       "58  chr16  68847694  68847694     G   T     intronic         CDH1   \n",
       "59  chr16  68847718  68847718     T   C     intronic         CDH1   \n",
       "60  chr16  68847719  68847719     C   A     intronic         CDH1   \n",
       "61  chr16  68847726  68847726     T   C     intronic         CDH1   \n",
       "62  chr16  68847727  68847727     A   T     intronic         CDH1   \n",
       "63  chr16  68855852  68855852     G   C     intronic         CDH1   \n",
       "64  chr16  68856474  68856474     T   C     intronic         CDH1   \n",
       "66  chr16  68857792  68857792     A   G     intronic         CDH1   \n",
       "67  chr16  68867612  68867612     T   -         UTR3         CDH1   \n",
       "68  chr17   7576265   7576265     A   -     intronic         TP53   \n",
       "69  chr17   7576443   7576443     -  AA     intronic         TP53   \n",
       "73  chr17   7578712   7578715  TTTT   -         UTR5         TP53   \n",
       "79  chr17   7590593   7590593     A   C     intronic  TP53;WRAP53   \n",
       "80  chr17   7590601   7590601     A   C     intronic  TP53;WRAP53   \n",
       "81  chr17   7590607   7590607     A   C     intronic  TP53;WRAP53   \n",
       "82  chr17  41196822  41196824   TTT   -         UTR3        BRCA1   \n",
       "84  chr17  41247621  41247621     A   -     intronic        BRCA1   \n",
       "86  chr17  41256088  41256088     A   -     intronic        BRCA1   \n",
       "87  chr17  41258136  41258136     A   -     intronic        BRCA1   \n",
       "88  chr19   1206797   1206797     T   -         UTR5        STK11   \n",
       "89  chr19   1219444   1219444     C   G     intronic        STK11   \n",
       "90  chr19   1219451   1219451     C   G     intronic        STK11   \n",
       "\n",
       "                                   GeneDetail.refGene   ExonicFunc.refGene  \\\n",
       "0                              NM_001126049:c.-694T>G                    .   \n",
       "1                              NM_001126049:c.-898G>A                    .   \n",
       "4                                                   .                    .   \n",
       "5                                                   .                    .   \n",
       "6                                                   .                    .   \n",
       "7                                                   .                    .   \n",
       "8                                                   .                    .   \n",
       "9                                                   .                    .   \n",
       "12  NM_001304718:c.*65delT;NM_001304717:c.*65delT;...                    .   \n",
       "13  NM_001304718:c.*353C>T;NM_001304717:c.*353C>T;...                    .   \n",
       "17                                                  .                    .   \n",
       "19                                                  .                    .   \n",
       "30                                                  .  frameshift deletion   \n",
       "33                                                  .                    .   \n",
       "40                                                  .                    .   \n",
       "48                                                  .                    .   \n",
       "50                                            dist=71                    .   \n",
       "55                                                  .                    .   \n",
       "56                                                  .                    .   \n",
       "57                                                  .                    .   \n",
       "58                                                  .                    .   \n",
       "59                                                  .                    .   \n",
       "60                                                  .                    .   \n",
       "61                                                  .                    .   \n",
       "62                                                  .                    .   \n",
       "63                                                  .                    .   \n",
       "64                                                  .                    .   \n",
       "66                                                  .                    .   \n",
       "67  NM_004360:c.*210delT;NM_001317186:c.*210delT;N...                    .   \n",
       "68                                                  .                    .   \n",
       "69                                                  .                    .   \n",
       "73  NM_001276697:c.-260_-263delAAAA;NM_001276698:c...                    .   \n",
       "79                                                  .                    .   \n",
       "80                                                  .                    .   \n",
       "81                                                  .                    .   \n",
       "82  NM_007300:c.*873_*871delAAA;NM_007297:c.*873_*...                    .   \n",
       "84                                                  .                    .   \n",
       "86                                                  .                    .   \n",
       "87                                                  .                    .   \n",
       "88                               NM_000455:c.-116del-                    .   \n",
       "89                                                  .                    .   \n",
       "90                                                  .                    .   \n",
       "\n",
       "                                   AAChange.refGene  ... VCF_CHR   VCF_POS  \\\n",
       "0                                                 .  ...   chr10  89622938   \n",
       "1                                                 .  ...   chr10  89623142   \n",
       "4                                                 .  ...   chr10  89654121   \n",
       "5                                                 .  ...   chr10  89654190   \n",
       "6                                                 .  ...   chr10  89711576   \n",
       "7                                                 .  ...   chr10  89718057   \n",
       "8                                                 .  ...   chr10  89720548   \n",
       "9                                                 .  ...   chr10  89720633   \n",
       "12                                                .  ...   chr10  89725293   \n",
       "13                                                .  ...   chr10  89725582   \n",
       "17                                                .  ...   chr13  32905219   \n",
       "19                                                .  ...   chr13  32907535   \n",
       "30  BRCA2:NM_000059:exon14:c.7068_7069del:p.F2356fs  ...   chr13  32929057   \n",
       "33                                                .  ...   chr13  32930372   \n",
       "40                                                .  ...   chr13  32954692   \n",
       "48                                                .  ...   chr16  23640160   \n",
       "50                                                .  ...   chr16  68771122   \n",
       "55                                                .  ...   chr16  68820717   \n",
       "56                                                .  ...   chr16  68847691   \n",
       "57                                                .  ...   chr16  68847692   \n",
       "58                                                .  ...   chr16  68847694   \n",
       "59                                                .  ...   chr16  68847718   \n",
       "60                                                .  ...   chr16  68847719   \n",
       "61                                                .  ...   chr16  68847726   \n",
       "62                                                .  ...   chr16  68847727   \n",
       "63                                                .  ...   chr16  68855852   \n",
       "64                                                .  ...   chr16  68856474   \n",
       "66                                                .  ...   chr16  68857792   \n",
       "67                                                .  ...   chr16  68867611   \n",
       "68                                                .  ...   chr17   7576264   \n",
       "69                                                .  ...   chr17   7576443   \n",
       "73                                                .  ...   chr17   7578711   \n",
       "79                                                .  ...   chr17   7590593   \n",
       "80                                                .  ...   chr17   7590601   \n",
       "81                                                .  ...   chr17   7590607   \n",
       "82                                                .  ...   chr17  41196821   \n",
       "84                                                .  ...   chr17  41247620   \n",
       "86                                                .  ...   chr17  41256087   \n",
       "87                                                .  ...   chr17  41258135   \n",
       "88                                                .  ...   chr19   1206796   \n",
       "89                                                .  ...   chr19   1219444   \n",
       "90                                                .  ...   chr19   1219451   \n",
       "\n",
       "         VCF_ID VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "0             .       A       C     290.77          .   \n",
       "1             .       C       T    7963.77          .   \n",
       "4   rs139651072       T       C     216.77          .   \n",
       "5   rs181780682       G       A      29.77          .   \n",
       "6   rs141005791       C       T      96.77          .   \n",
       "7             .       A       G     759.77          .   \n",
       "8             .       A       G    1491.77          .   \n",
       "9             .      CT       C    1805.73          .   \n",
       "12            .      CT       C    9448.73          .   \n",
       "13  rs181234898       C       T     201.77          .   \n",
       "17            .      AT       A    2225.73          .   \n",
       "19            .      CT       C    4200.73          .   \n",
       "30   rs80359636     TTC       T   33599.70          .   \n",
       "33            .      AT       A     119.73          .   \n",
       "40            .       C       T      21.80          .   \n",
       "48            .       G       T      35.77          .   \n",
       "50   rs28372783       A       C   18862.80          .   \n",
       "55  rs146972234       C       A     680.77          .   \n",
       "56            .       C       T      28.77          .   \n",
       "57            .       A       G      28.77          .   \n",
       "58            .       G       T      28.77          .   \n",
       "59            .       T       C      34.77          .   \n",
       "60            .       C       A      23.79          .   \n",
       "61            .       T       C      34.77          .   \n",
       "62            .       A       T      23.79          .   \n",
       "63   rs34582129       G       C    9012.77          .   \n",
       "64            .       T       C      38.77          .   \n",
       "66            .       A       G      22.79          .   \n",
       "67            .      CT       C     986.73          .   \n",
       "68            .      TA       T     105.73          .   \n",
       "69            .       C     CAA     605.73          .   \n",
       "73            .   CTTTT       C   78271.70          .   \n",
       "79            .       A       C      87.77          .   \n",
       "80            .       A       C     339.77          .   \n",
       "81            .       A       C      41.77          .   \n",
       "82            .    CTTT       C      84.73          .   \n",
       "84            .      CA       C     197.73          .   \n",
       "86            .      GA       G     357.73          .   \n",
       "87            .      TA       T     203.73          .   \n",
       "88            .      CT       C    2222.73          .   \n",
       "89            .       C       G     672.77          .   \n",
       "90            .       C       G     213.77          .   \n",
       "\n",
       "                                             VCF_INFO  \\\n",
       "0   ABHet=0.696;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....   \n",
       "1   ABHet=0.534;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...   \n",
       "4   ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...   \n",
       "5   ABHet=0.714;AC=1;AF=0.5;AN=2;BaseQRankSum=0.36...   \n",
       "6   ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.21...   \n",
       "7   ABHet=0.324;AC=1;AF=0.5;AN=2;BaseQRankSum=0.83...   \n",
       "8   ABHet=0.467;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.5...   \n",
       "9   AC=1;AF=0.5;AN=2;BaseQRankSum=-0.601;DP=689;Ex...   \n",
       "12  AC=1;AF=0.5;AN=2;BaseQRankSum=0.417;DP=2406;Ex...   \n",
       "13  ABHet=0.526;AC=1;AF=0.5;AN=2;BaseQRankSum=0.31...   \n",
       "17  AC=1;AF=0.5;AN=2;BaseQRankSum=4.537;DP=630;Exc...   \n",
       "19  AC=1;AF=0.5;AN=2;BaseQRankSum=3.476;DP=1016;Ex...   \n",
       "30  AC=1;AF=0.5;AN=2;BaseQRankSum=2.352;DB;DP=1613...   \n",
       "33  AC=1;AF=0.5;AN=2;BaseQRankSum=0.879;ClippingRa...   \n",
       "40  ABHet=0.625;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.2...   \n",
       "48  ABHet=0.6;AC=1;AF=0.5;AN=2;BaseQRankSum=0;DP=5...   \n",
       "50  ABHet=0.507;AC=1;AF=0.5;AN=2;BaseQRankSum=11.1...   \n",
       "55  ABHet=0.587;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.4...   \n",
       "56  ABHet=0.818;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.8...   \n",
       "57  ABHet=0.818;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.7...   \n",
       "58  ABHet=0.818;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.8...   \n",
       "59  ABHet=0.778;AC=1;AF=0.5;AN=2;BaseQRankSum=0.03...   \n",
       "60  ABHet=0.8;AC=1;AF=0.5;AN=2;BaseQRankSum=0.728;...   \n",
       "61  ABHet=0.778;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.4...   \n",
       "62  ABHet=0.8;AC=1;AF=0.5;AN=2;BaseQRankSum=0.493;...   \n",
       "63  ABHet=0.517;AC=1;AF=0.5;AN=2;BaseQRankSum=0.26...   \n",
       "64  ABHet=0.6;AC=1;AF=0.5;AN=2;BaseQRankSum=0.524;...   \n",
       "66  ABHet=0.75;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.67...   \n",
       "67  AC=1;AF=0.5;AN=2;BaseQRankSum=1.16;DP=270;Exce...   \n",
       "68  AC=1;AF=0.5;AN=2;BaseQRankSum=0.354;DP=29;Exce...   \n",
       "69  AC=1;AF=0.5;AN=2;BaseQRankSum=-1.367;ClippingR...   \n",
       "73  AC=1;AF=0.5;AN=2;BaseQRankSum=-0.215;DP=2426;E...   \n",
       "79  ABHet=0.749;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.3...   \n",
       "80  ABHet=0.585;AC=1;AF=0.5;AN=2;BaseQRankSum=-2.0...   \n",
       "81  ABHet=0.704;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.0...   \n",
       "82  AC=1;AF=0.5;AN=2;BaseQRankSum=0.689;ClippingRa...   \n",
       "84  AC=1;AF=0.5;AN=2;BaseQRankSum=-0.097;DP=38;Exc...   \n",
       "86  AC=1;AF=0.5;AN=2;BaseQRankSum=-0.38;ClippingRa...   \n",
       "87  AC=1;AF=0.5;AN=2;BaseQRankSum=-1.019;DP=69;Exc...   \n",
       "88  AC=1;AF=0.5;AN=2;BaseQRankSum=1.767;ClippingRa...   \n",
       "89  ABHet=0.307;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.8...   \n",
       "90  ABHet=0.654;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.6...   \n",
       "\n",
       "                                VCF_FORMAT  \\\n",
       "0                           GT:AD:DP:GQ:PL   \n",
       "1                           GT:AD:DP:GQ:PL   \n",
       "4                           GT:AD:DP:GQ:PL   \n",
       "5                           GT:AD:DP:GQ:PL   \n",
       "6                           GT:AD:DP:GQ:PL   \n",
       "7                           GT:AD:DP:GQ:PL   \n",
       "8                           GT:AD:DP:GQ:PL   \n",
       "9                           GT:AD:DP:GQ:PL   \n",
       "12                          GT:AD:DP:GQ:PL   \n",
       "13                          GT:AD:DP:GQ:PL   \n",
       "17                          GT:AD:DP:GQ:PL   \n",
       "19                          GT:AD:DP:GQ:PL   \n",
       "30                          GT:AD:DP:GQ:PL   \n",
       "33     GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "40  GT:AB:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "48                          GT:AD:DP:GQ:PL   \n",
       "50                          GT:AD:DP:GQ:PL   \n",
       "55                          GT:AD:DP:GQ:PL   \n",
       "56  GT:AB:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "57  GT:AB:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "58  GT:AB:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "59  GT:AB:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "60                          GT:AD:DP:GQ:PL   \n",
       "61  GT:AB:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "62                          GT:AD:DP:GQ:PL   \n",
       "63                          GT:AD:DP:GQ:PL   \n",
       "64                          GT:AD:DP:GQ:PL   \n",
       "66                          GT:AD:DP:GQ:PL   \n",
       "67                          GT:AD:DP:GQ:PL   \n",
       "68                          GT:AD:DP:GQ:PL   \n",
       "69     GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "73                          GT:AD:DP:GQ:PL   \n",
       "79                          GT:AD:DP:GQ:PL   \n",
       "80                          GT:AD:DP:GQ:PL   \n",
       "81                          GT:AD:DP:GQ:PL   \n",
       "82     GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "84                          GT:AD:DP:GQ:PL   \n",
       "86     GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "87                          GT:AD:DP:GQ:PL   \n",
       "88     GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "89                          GT:AD:DP:GQ:PL   \n",
       "90                          GT:AD:DP:GQ:PL   \n",
       "\n",
       "                                      VCF_SAMPLE_NAME  \n",
       "0                    0/1:1266,552:1820:99:319,0,12285  \n",
       "1                      0/1:306,267:574:99:7992,0,9864  \n",
       "4                            0/1:10,8:18:99:245,0,289  \n",
       "5                               0/1:5,2:7:58:58,0,172  \n",
       "6                              0/1:5,4:9:99:125,0,133  \n",
       "7                           0/1:11,23:34:99:788,0,283  \n",
       "8                         0/1:42,48:90:99:1520,0,1330  \n",
       "9                      0/1:443,192:689:99:1843,0,6330  \n",
       "12                  0/1:1610,625:2406:99:9486,0,34365  \n",
       "13                           0/1:10,9:19:99:230,0,266  \n",
       "17                     0/1:413,156:630:99:2263,0,8472  \n",
       "19                   0/1:669,272:1016:99:4238,0,14585  \n",
       "30                  0/1:853,734:1613:99:33637,0,39748  \n",
       "33  0/1:27,10:37:99:1:0.5:157,0,642:27,0,10,0:27,0...  \n",
       "40    0/1:0.63:5,3:8:50:1:0.5:50,0,94:0,5,0,3:0,5,0,3  \n",
       "48                              0/1:3,2:5:64:64,0,103  \n",
       "50                  0/1:685,666:1355:99:18891,0,17969  \n",
       "55                         0/1:37,26:63:99:709,0,1225  \n",
       "56  0/1:0.82:9,2:11:57:1:0.5:57,0,1646:0,9,0,2:0,9...  \n",
       "57  0/1:0.82:9,2:11:57:1:0.5:57,0,1646:0,9,0,2:0,9...  \n",
       "58  0/1:0.82:9,2:11:57:1:0.5:57,0,1646:0,9,0,2:0,9...  \n",
       "59  0/1:0.78:7,2:9:63:1:0.5:63,0,1502:0,7,0,2:0,7,0,2  \n",
       "60                             0/1:8,2:10:52:52,0,233  \n",
       "61  0/1:0.78:7,2:9:63:1:0.5:63,0,1502:0,7,0,2:0,7,0,2  \n",
       "62                             0/1:8,2:10:52:52,0,245  \n",
       "63                     0/1:312,292:606:99:9041,0,9787  \n",
       "64                               0/1:3,2:5:67:67,0,67  \n",
       "66                              0/1:6,2:8:51:51,0,209  \n",
       "67                      0/1:161,71:270:99:1024,0,3177  \n",
       "68                           0/1:20,9:29:99:143,0,380  \n",
       "69  0/1:11,24:35:99:1:0.5:566,0,111:11,0,24,0:10,0...  \n",
       "73             1/.:152,1138:2426:99:78309,15975,16093  \n",
       "79                        0/1:188,63:251:99:116,0,652  \n",
       "80                      0/1:172,122:294:99:368,0,1128  \n",
       "81                        0/1:226,95:321:70:70,0,2914  \n",
       "82  0/1:109,19:128:99:1:0.5:122,0,3145:69,40,12,7:...  \n",
       "84                          0/1:24,13:38:99:235,0,434  \n",
       "86  0/1:83,28:111:99:1:0.5:395,0,2036:83,0,28,0:70...  \n",
       "87                          0/1:36,24:69:99:241,0,424  \n",
       "88  0/1:604,172:776:99:1:0.5:2260,0,15656:245,359,...  \n",
       "89                         0/1:39,88:127:99:701,0,458  \n",
       "90                       0/1:117,62:179:99:242,0,1041  \n",
       "\n",
       "[42 rows x 183 columns]"
      ]
     },
     "execution_count": 59,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "str_filter = multianno_df[\"HIAE_EXOME_FREQUENCY(722)\"] < 0.05\n",
    "multianno_df[str_filter]"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Vamos olhar pros genes agora"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 60,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "array(['KLLN', 'PTEN', 'BRCA2', 'PALB2', 'CDH1', 'TP53', 'TP53;WRAP53',\n",
       "       'BRCA1', 'STK11'], dtype=object)"
      ]
     },
     "execution_count": 60,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Quais os possíveis genes com variantes nesse paciente?\n",
    "multianno_df['Gene.refGene'].unique()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 61,
   "metadata": {
    "scrolled": true
   },
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>Func.refGene</th>\n",
       "      <th>Gene.refGene</th>\n",
       "      <th>GeneDetail.refGene</th>\n",
       "      <th>ExonicFunc.refGene</th>\n",
       "      <th>AAChange.refGene</th>\n",
       "      <th>...</th>\n",
       "      <th>VCF_CHR</th>\n",
       "      <th>VCF_POS</th>\n",
       "      <th>VCF_ID</th>\n",
       "      <th>VCF_REF</th>\n",
       "      <th>VCF_ALT</th>\n",
       "      <th>VCF_QUAL_2</th>\n",
       "      <th>VCF_FILTER</th>\n",
       "      <th>VCF_INFO</th>\n",
       "      <th>VCF_FORMAT</th>\n",
       "      <th>VCF_SAMPLE_NAME</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>14</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32889968</td>\n",
       "      <td>32889968</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32889968</td>\n",
       "      <td>rs206119</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>3707.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=98;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,98:98:99:3736,289,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>15</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32899567</td>\n",
       "      <td>32899567</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32899567</td>\n",
       "      <td>rs11571613</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>634.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.286;AC=1;AF=0.5;AN=2;BaseQRankSum=0.96...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:8,20:28:99:663,0,227</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>16</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32899878</td>\n",
       "      <td>32899878</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32899878</td>\n",
       "      <td>rs206071</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>633.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=19;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,19:19:51:662,51,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>17</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32905220</td>\n",
       "      <td>32905220</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32905219</td>\n",
       "      <td>.</td>\n",
       "      <td>AT</td>\n",
       "      <td>A</td>\n",
       "      <td>2225.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=4.537;DP=630;Exc...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:413,156:630:99:2263,0,8472</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>18</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32905265</td>\n",
       "      <td>32905265</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32905265</td>\n",
       "      <td>rs206073</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>14280.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=0.995;AC=2;AF=1;AN=2;BaseQRankSum=-0.511...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:2,388:392:99:14309,1038,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>19</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32907536</td>\n",
       "      <td>32907536</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32907535</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>4200.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=3.476;DP=1016;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:669,272:1016:99:4238,0,14585</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>20</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32911888</td>\n",
       "      <td>32911888</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>exonic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>synonymous SNV</td>\n",
       "      <td>BRCA2:NM_000059:exon11:c.3396A&gt;G:p.K1132K</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32911888</td>\n",
       "      <td>rs1801406</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>30677.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.511;AC=1;AF=0.5;AN=2;BaseQRankSum=1.67...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1393,1332:2730:99:30706,0,32616</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>21</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32912299</td>\n",
       "      <td>32912299</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>exonic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>synonymous SNV</td>\n",
       "      <td>BRCA2:NM_000059:exon11:c.3807T&gt;C:p.V1269V</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32912299</td>\n",
       "      <td>rs543304</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>35335.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.503;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1388,1370:2759:99:35364,0,36070</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>22</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32913055</td>\n",
       "      <td>32913055</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>exonic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>synonymous SNV</td>\n",
       "      <td>BRCA2:NM_000059:exon11:c.4563A&gt;G:p.L1521L</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32913055</td>\n",
       "      <td>rs206075</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>53264.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=2013;Dels=0;Exces...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,2010:2013:99:53293,3847,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>23</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32915005</td>\n",
       "      <td>32915005</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>exonic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>synonymous SNV</td>\n",
       "      <td>BRCA2:NM_000059:exon11:c.6513G&gt;C:p.V2171V</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32915005</td>\n",
       "      <td>rs206076</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>103249.00</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=3126;Dels=0;Exces...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,3123:3126:99:103277,7777,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>24</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32915411</td>\n",
       "      <td>32915414</td>\n",
       "      <td>AATT</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32915410</td>\n",
       "      <td>rs11571661</td>\n",
       "      <td>CAATT</td>\n",
       "      <td>C</td>\n",
       "      <td>18957.70</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.224;DB;DP=651;...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:281,299:651:99:18995,0,18017</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>25</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32915524</td>\n",
       "      <td>32915524</td>\n",
       "      <td>C</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32915524</td>\n",
       "      <td>rs11571662</td>\n",
       "      <td>C</td>\n",
       "      <td>A</td>\n",
       "      <td>1868.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.433;AC=1;AF=0.5;AN=2;BaseQRankSum=1.44...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:45,59:105:99:1897,0,1265</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>26</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32919637</td>\n",
       "      <td>32919637</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32919637</td>\n",
       "      <td>rs9567576</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>21775.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.497;AC=1;AF=0.5;AN=2;BaseQRankSum=2.43...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1006,1020:2031:99:21804,0,22434</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>27</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32920016</td>\n",
       "      <td>32920016</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32920016</td>\n",
       "      <td>rs206078</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>207.84</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=6;Dels=0;ExcessHe...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,6:6:18:236,18,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>28</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32920618</td>\n",
       "      <td>32920618</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32920618</td>\n",
       "      <td>rs206079</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>573.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.429;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.7...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:15,20:35:99:602,0,490</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>29</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32920844</td>\n",
       "      <td>32920844</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32920844</td>\n",
       "      <td>rs206080</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>23511.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=623;Dels=0;Excess...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,622:623:99:23540,1833,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>30</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32929058</td>\n",
       "      <td>32929059</td>\n",
       "      <td>TC</td>\n",
       "      <td>-</td>\n",
       "      <td>exonic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>frameshift deletion</td>\n",
       "      <td>BRCA2:NM_000059:exon14:c.7068_7069del:p.F2356fs</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32929057</td>\n",
       "      <td>rs80359636</td>\n",
       "      <td>TTC</td>\n",
       "      <td>T</td>\n",
       "      <td>33599.70</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=2.352;DB;DP=1613...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:853,734:1613:99:33637,0,39748</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>31</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32929232</td>\n",
       "      <td>32929232</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>exonic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>synonymous SNV</td>\n",
       "      <td>BRCA2:NM_000059:exon14:c.7242A&gt;G:p.S2414S</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32929232</td>\n",
       "      <td>rs1799955</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>34495.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.443;AC=1;AF=0.5;AN=2;BaseQRankSum=-8.5...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1114,1403:2524:99:34524,0,24892</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>32</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32929387</td>\n",
       "      <td>32929387</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>exonic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>nonsynonymous SNV</td>\n",
       "      <td>BRCA2:NM_000059:exon14:c.7397T&gt;C:p.V2466A</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32929387</td>\n",
       "      <td>rs169547</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>61976.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=0.999;AC=2;AF=1;AN=2;BaseQRankSum=-1.091...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:3,2384:2392:99:62005,4418,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>33</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32930373</td>\n",
       "      <td>32930373</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32930372</td>\n",
       "      <td>.</td>\n",
       "      <td>AT</td>\n",
       "      <td>A</td>\n",
       "      <td>119.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.879;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:27,10:37:99:1:0.5:157,0,642:27,0,10,0:27,0...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>34</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32936646</td>\n",
       "      <td>32936646</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32936646</td>\n",
       "      <td>rs9534262</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>25543.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.527;AC=1;AF=0.5;AN=2;BaseQRankSum=-9.4...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1179,1058:2247:99:25572,0,30515</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>35</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32950658</td>\n",
       "      <td>32950658</td>\n",
       "      <td>-</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32950658</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>CA</td>\n",
       "      <td>309.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=1.494;DP=85;Exce...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:50,29:85:99:347,0,738</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>36</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32951111</td>\n",
       "      <td>32951111</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32951111</td>\n",
       "      <td>rs3764791</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>174.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.417;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.1...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:5,7:12:99:203,0,160</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>37</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32951115</td>\n",
       "      <td>32951115</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32951115</td>\n",
       "      <td>rs3764792</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>65.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.0...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:5,4:9:94:94,0,162</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>38</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32953388</td>\n",
       "      <td>32953388</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32953388</td>\n",
       "      <td>rs4942486</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>6767.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.51;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.58...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:224,215:440:99:6796,0,7155</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>39</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32954561</td>\n",
       "      <td>32954561</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32954561</td>\n",
       "      <td>rs7328654</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>2069.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.369;AC=1;AF=0.5;AN=2;BaseQRankSum=1.03...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:38,65:103:99:2098,0,1050</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>40</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32954692</td>\n",
       "      <td>32954692</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32954692</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>21.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.625;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.2...</td>\n",
       "      <td>GT:AB:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:0.63:5,3:8:50:1:0.5:50,0,94:0,5,0,3:0,5,0,3</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>41</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32954741</td>\n",
       "      <td>32954741</td>\n",
       "      <td>T</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32954741</td>\n",
       "      <td>rs206145</td>\n",
       "      <td>T</td>\n",
       "      <td>A</td>\n",
       "      <td>161.90</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=5;Dels=0;ExcessHe...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,5:5:15:190,15,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>42</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32968591</td>\n",
       "      <td>32968591</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32968591</td>\n",
       "      <td>rs7327867</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>435.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.36;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.43...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:9,16:25:99:464,0,245</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>43</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32968607</td>\n",
       "      <td>32968607</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32968607</td>\n",
       "      <td>rs7330025</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>677.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.4;AC=1;AF=0.5;AN=2;BaseQRankSum=0.242;...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:14,21:35:99:706,0,402</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>44</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32970736</td>\n",
       "      <td>32970736</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32970736</td>\n",
       "      <td>rs1012129</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>563.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.528;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.4...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:28,25:53:99:592,0,910</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>45</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32971425</td>\n",
       "      <td>32971425</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32971425</td>\n",
       "      <td>rs206344</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>841.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=24;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,24:24:69:870,69,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>46</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32973012</td>\n",
       "      <td>32973012</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>BRCA2</td>\n",
       "      <td>NM_000059:c.*105A&gt;C</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr13</td>\n",
       "      <td>32973012</td>\n",
       "      <td>rs15869</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>4039.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.511;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.6...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:137,131:268:99:4068,0,4525</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>33 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr     Start       End   Ref Alt Func.refGene Gene.refGene  \\\n",
       "14  chr13  32889968  32889968     G   A     intronic        BRCA2   \n",
       "15  chr13  32899567  32899567     G   C     intronic        BRCA2   \n",
       "16  chr13  32899878  32899878     C   T     intronic        BRCA2   \n",
       "17  chr13  32905220  32905220     T   -     intronic        BRCA2   \n",
       "18  chr13  32905265  32905265     G   A     intronic        BRCA2   \n",
       "19  chr13  32907536  32907536     T   -     intronic        BRCA2   \n",
       "20  chr13  32911888  32911888     A   G       exonic        BRCA2   \n",
       "21  chr13  32912299  32912299     T   C       exonic        BRCA2   \n",
       "22  chr13  32913055  32913055     A   G       exonic        BRCA2   \n",
       "23  chr13  32915005  32915005     G   C       exonic        BRCA2   \n",
       "24  chr13  32915411  32915414  AATT   -     intronic        BRCA2   \n",
       "25  chr13  32915524  32915524     C   A     intronic        BRCA2   \n",
       "26  chr13  32919637  32919637     T   G     intronic        BRCA2   \n",
       "27  chr13  32920016  32920016     G   A     intronic        BRCA2   \n",
       "28  chr13  32920618  32920618     G   A     intronic        BRCA2   \n",
       "29  chr13  32920844  32920844     T   C     intronic        BRCA2   \n",
       "30  chr13  32929058  32929059    TC   -       exonic        BRCA2   \n",
       "31  chr13  32929232  32929232     A   G       exonic        BRCA2   \n",
       "32  chr13  32929387  32929387     T   C       exonic        BRCA2   \n",
       "33  chr13  32930373  32930373     T   -     intronic        BRCA2   \n",
       "34  chr13  32936646  32936646     T   C     intronic        BRCA2   \n",
       "35  chr13  32950658  32950658     -   A     intronic        BRCA2   \n",
       "36  chr13  32951111  32951111     A   C     intronic        BRCA2   \n",
       "37  chr13  32951115  32951115     C   T     intronic        BRCA2   \n",
       "38  chr13  32953388  32953388     T   C     intronic        BRCA2   \n",
       "39  chr13  32954561  32954561     G   A     intronic        BRCA2   \n",
       "40  chr13  32954692  32954692     C   T     intronic        BRCA2   \n",
       "41  chr13  32954741  32954741     T   A     intronic        BRCA2   \n",
       "42  chr13  32968591  32968591     G   A     intronic        BRCA2   \n",
       "43  chr13  32968607  32968607     A   G     intronic        BRCA2   \n",
       "44  chr13  32970736  32970736     G   C     intronic        BRCA2   \n",
       "45  chr13  32971425  32971425     T   C     intronic        BRCA2   \n",
       "46  chr13  32973012  32973012     A   C         UTR3        BRCA2   \n",
       "\n",
       "     GeneDetail.refGene   ExonicFunc.refGene  \\\n",
       "14                    .                    .   \n",
       "15                    .                    .   \n",
       "16                    .                    .   \n",
       "17                    .                    .   \n",
       "18                    .                    .   \n",
       "19                    .                    .   \n",
       "20                    .       synonymous SNV   \n",
       "21                    .       synonymous SNV   \n",
       "22                    .       synonymous SNV   \n",
       "23                    .       synonymous SNV   \n",
       "24                    .                    .   \n",
       "25                    .                    .   \n",
       "26                    .                    .   \n",
       "27                    .                    .   \n",
       "28                    .                    .   \n",
       "29                    .                    .   \n",
       "30                    .  frameshift deletion   \n",
       "31                    .       synonymous SNV   \n",
       "32                    .    nonsynonymous SNV   \n",
       "33                    .                    .   \n",
       "34                    .                    .   \n",
       "35                    .                    .   \n",
       "36                    .                    .   \n",
       "37                    .                    .   \n",
       "38                    .                    .   \n",
       "39                    .                    .   \n",
       "40                    .                    .   \n",
       "41                    .                    .   \n",
       "42                    .                    .   \n",
       "43                    .                    .   \n",
       "44                    .                    .   \n",
       "45                    .                    .   \n",
       "46  NM_000059:c.*105A>C                    .   \n",
       "\n",
       "                                   AAChange.refGene  ... VCF_CHR   VCF_POS  \\\n",
       "14                                                .  ...   chr13  32889968   \n",
       "15                                                .  ...   chr13  32899567   \n",
       "16                                                .  ...   chr13  32899878   \n",
       "17                                                .  ...   chr13  32905219   \n",
       "18                                                .  ...   chr13  32905265   \n",
       "19                                                .  ...   chr13  32907535   \n",
       "20        BRCA2:NM_000059:exon11:c.3396A>G:p.K1132K  ...   chr13  32911888   \n",
       "21        BRCA2:NM_000059:exon11:c.3807T>C:p.V1269V  ...   chr13  32912299   \n",
       "22        BRCA2:NM_000059:exon11:c.4563A>G:p.L1521L  ...   chr13  32913055   \n",
       "23        BRCA2:NM_000059:exon11:c.6513G>C:p.V2171V  ...   chr13  32915005   \n",
       "24                                                .  ...   chr13  32915410   \n",
       "25                                                .  ...   chr13  32915524   \n",
       "26                                                .  ...   chr13  32919637   \n",
       "27                                                .  ...   chr13  32920016   \n",
       "28                                                .  ...   chr13  32920618   \n",
       "29                                                .  ...   chr13  32920844   \n",
       "30  BRCA2:NM_000059:exon14:c.7068_7069del:p.F2356fs  ...   chr13  32929057   \n",
       "31        BRCA2:NM_000059:exon14:c.7242A>G:p.S2414S  ...   chr13  32929232   \n",
       "32        BRCA2:NM_000059:exon14:c.7397T>C:p.V2466A  ...   chr13  32929387   \n",
       "33                                                .  ...   chr13  32930372   \n",
       "34                                                .  ...   chr13  32936646   \n",
       "35                                                .  ...   chr13  32950658   \n",
       "36                                                .  ...   chr13  32951111   \n",
       "37                                                .  ...   chr13  32951115   \n",
       "38                                                .  ...   chr13  32953388   \n",
       "39                                                .  ...   chr13  32954561   \n",
       "40                                                .  ...   chr13  32954692   \n",
       "41                                                .  ...   chr13  32954741   \n",
       "42                                                .  ...   chr13  32968591   \n",
       "43                                                .  ...   chr13  32968607   \n",
       "44                                                .  ...   chr13  32970736   \n",
       "45                                                .  ...   chr13  32971425   \n",
       "46                                                .  ...   chr13  32973012   \n",
       "\n",
       "        VCF_ID VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "14    rs206119       G       A    3707.77          .   \n",
       "15  rs11571613       G       C     634.77          .   \n",
       "16    rs206071       C       T     633.77          .   \n",
       "17           .      AT       A    2225.73          .   \n",
       "18    rs206073       G       A   14280.80          .   \n",
       "19           .      CT       C    4200.73          .   \n",
       "20   rs1801406       A       G   30677.80          .   \n",
       "21    rs543304       T       C   35335.80          .   \n",
       "22    rs206075       A       G   53264.80          .   \n",
       "23    rs206076       G       C  103249.00          .   \n",
       "24  rs11571661   CAATT       C   18957.70          .   \n",
       "25  rs11571662       C       A    1868.77          .   \n",
       "26   rs9567576       T       G   21775.80          .   \n",
       "27    rs206078       G       A     207.84          .   \n",
       "28    rs206079       G       A     573.77          .   \n",
       "29    rs206080       T       C   23511.80          .   \n",
       "30  rs80359636     TTC       T   33599.70          .   \n",
       "31   rs1799955       A       G   34495.80          .   \n",
       "32    rs169547       T       C   61976.80          .   \n",
       "33           .      AT       A     119.73          .   \n",
       "34   rs9534262       T       C   25543.80          .   \n",
       "35           .       C      CA     309.73          .   \n",
       "36   rs3764791       A       C     174.77          .   \n",
       "37   rs3764792       C       T      65.77          .   \n",
       "38   rs4942486       T       C    6767.77          .   \n",
       "39   rs7328654       G       A    2069.77          .   \n",
       "40           .       C       T      21.80          .   \n",
       "41    rs206145       T       A     161.90          .   \n",
       "42   rs7327867       G       A     435.77          .   \n",
       "43   rs7330025       A       G     677.77          .   \n",
       "44   rs1012129       G       C     563.77          .   \n",
       "45    rs206344       T       C     841.77          .   \n",
       "46     rs15869       A       C    4039.77          .   \n",
       "\n",
       "                                             VCF_INFO  \\\n",
       "14  ABHom=1;AC=2;AF=1;AN=2;DB;DP=98;Dels=0;ExcessH...   \n",
       "15  ABHet=0.286;AC=1;AF=0.5;AN=2;BaseQRankSum=0.96...   \n",
       "16  ABHom=1;AC=2;AF=1;AN=2;DB;DP=19;Dels=0;ExcessH...   \n",
       "17  AC=1;AF=0.5;AN=2;BaseQRankSum=4.537;DP=630;Exc...   \n",
       "18  ABHom=0.995;AC=2;AF=1;AN=2;BaseQRankSum=-0.511...   \n",
       "19  AC=1;AF=0.5;AN=2;BaseQRankSum=3.476;DP=1016;Ex...   \n",
       "20  ABHet=0.511;AC=1;AF=0.5;AN=2;BaseQRankSum=1.67...   \n",
       "21  ABHet=0.503;AC=1;AF=0.5;AN=2;BaseQRankSum=-10....   \n",
       "22  ABHom=1;AC=2;AF=1;AN=2;DB;DP=2013;Dels=0;Exces...   \n",
       "23  ABHom=1;AC=2;AF=1;AN=2;DB;DP=3126;Dels=0;Exces...   \n",
       "24  AC=1;AF=0.5;AN=2;BaseQRankSum=0.224;DB;DP=651;...   \n",
       "25  ABHet=0.433;AC=1;AF=0.5;AN=2;BaseQRankSum=1.44...   \n",
       "26  ABHet=0.497;AC=1;AF=0.5;AN=2;BaseQRankSum=2.43...   \n",
       "27  ABHom=1;AC=2;AF=1;AN=2;DB;DP=6;Dels=0;ExcessHe...   \n",
       "28  ABHet=0.429;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.7...   \n",
       "29  ABHom=1;AC=2;AF=1;AN=2;DB;DP=623;Dels=0;Excess...   \n",
       "30  AC=1;AF=0.5;AN=2;BaseQRankSum=2.352;DB;DP=1613...   \n",
       "31  ABHet=0.443;AC=1;AF=0.5;AN=2;BaseQRankSum=-8.5...   \n",
       "32  ABHom=0.999;AC=2;AF=1;AN=2;BaseQRankSum=-1.091...   \n",
       "33  AC=1;AF=0.5;AN=2;BaseQRankSum=0.879;ClippingRa...   \n",
       "34  ABHet=0.527;AC=1;AF=0.5;AN=2;BaseQRankSum=-9.4...   \n",
       "35  AC=1;AF=0.5;AN=2;BaseQRankSum=1.494;DP=85;Exce...   \n",
       "36  ABHet=0.417;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.1...   \n",
       "37  ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.0...   \n",
       "38  ABHet=0.51;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.58...   \n",
       "39  ABHet=0.369;AC=1;AF=0.5;AN=2;BaseQRankSum=1.03...   \n",
       "40  ABHet=0.625;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.2...   \n",
       "41  ABHom=1;AC=2;AF=1;AN=2;DB;DP=5;Dels=0;ExcessHe...   \n",
       "42  ABHet=0.36;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.43...   \n",
       "43  ABHet=0.4;AC=1;AF=0.5;AN=2;BaseQRankSum=0.242;...   \n",
       "44  ABHet=0.528;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.4...   \n",
       "45  ABHom=1;AC=2;AF=1;AN=2;DB;DP=24;Dels=0;ExcessH...   \n",
       "46  ABHet=0.511;AC=1;AF=0.5;AN=2;BaseQRankSum=-1.6...   \n",
       "\n",
       "                                VCF_FORMAT  \\\n",
       "14                          GT:AD:DP:GQ:PL   \n",
       "15                          GT:AD:DP:GQ:PL   \n",
       "16                          GT:AD:DP:GQ:PL   \n",
       "17                          GT:AD:DP:GQ:PL   \n",
       "18                          GT:AD:DP:GQ:PL   \n",
       "19                          GT:AD:DP:GQ:PL   \n",
       "20                          GT:AD:DP:GQ:PL   \n",
       "21                          GT:AD:DP:GQ:PL   \n",
       "22                          GT:AD:DP:GQ:PL   \n",
       "23                          GT:AD:DP:GQ:PL   \n",
       "24                          GT:AD:DP:GQ:PL   \n",
       "25                          GT:AD:DP:GQ:PL   \n",
       "26                          GT:AD:DP:GQ:PL   \n",
       "27                          GT:AD:DP:GQ:PL   \n",
       "28                          GT:AD:DP:GQ:PL   \n",
       "29                          GT:AD:DP:GQ:PL   \n",
       "30                          GT:AD:DP:GQ:PL   \n",
       "31                          GT:AD:DP:GQ:PL   \n",
       "32                          GT:AD:DP:GQ:PL   \n",
       "33     GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "34                          GT:AD:DP:GQ:PL   \n",
       "35                          GT:AD:DP:GQ:PL   \n",
       "36                          GT:AD:DP:GQ:PL   \n",
       "37                          GT:AD:DP:GQ:PL   \n",
       "38                          GT:AD:DP:GQ:PL   \n",
       "39                          GT:AD:DP:GQ:PL   \n",
       "40  GT:AB:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "41                          GT:AD:DP:GQ:PL   \n",
       "42                          GT:AD:DP:GQ:PL   \n",
       "43                          GT:AD:DP:GQ:PL   \n",
       "44                          GT:AD:DP:GQ:PL   \n",
       "45                          GT:AD:DP:GQ:PL   \n",
       "46                          GT:AD:DP:GQ:PL   \n",
       "\n",
       "                                      VCF_SAMPLE_NAME  \n",
       "14                          1/1:0,98:98:99:3736,289,0  \n",
       "15                           0/1:8,20:28:99:663,0,227  \n",
       "16                            1/1:0,19:19:51:662,51,0  \n",
       "17                     0/1:413,156:630:99:2263,0,8472  \n",
       "18                      1/1:2,388:392:99:14309,1038,0  \n",
       "19                   0/1:669,272:1016:99:4238,0,14585  \n",
       "20                0/1:1393,1332:2730:99:30706,0,32616  \n",
       "21                0/1:1388,1370:2759:99:35364,0,36070  \n",
       "22                    1/1:0,2010:2013:99:53293,3847,0  \n",
       "23                   1/1:0,3123:3126:99:103277,7777,0  \n",
       "24                   0/1:281,299:651:99:18995,0,18017  \n",
       "25                       0/1:45,59:105:99:1897,0,1265  \n",
       "26                0/1:1006,1020:2031:99:21804,0,22434  \n",
       "27                              1/1:0,6:6:18:236,18,0  \n",
       "28                          0/1:15,20:35:99:602,0,490  \n",
       "29                      1/1:0,622:623:99:23540,1833,0  \n",
       "30                  0/1:853,734:1613:99:33637,0,39748  \n",
       "31                0/1:1114,1403:2524:99:34524,0,24892  \n",
       "32                    1/1:3,2384:2392:99:62005,4418,0  \n",
       "33  0/1:27,10:37:99:1:0.5:157,0,642:27,0,10,0:27,0...  \n",
       "34                0/1:1179,1058:2247:99:25572,0,30515  \n",
       "35                          0/1:50,29:85:99:347,0,738  \n",
       "36                            0/1:5,7:12:99:203,0,160  \n",
       "37                              0/1:5,4:9:94:94,0,162  \n",
       "38                     0/1:224,215:440:99:6796,0,7155  \n",
       "39                       0/1:38,65:103:99:2098,0,1050  \n",
       "40    0/1:0.63:5,3:8:50:1:0.5:50,0,94:0,5,0,3:0,5,0,3  \n",
       "41                              1/1:0,5:5:15:190,15,0  \n",
       "42                           0/1:9,16:25:99:464,0,245  \n",
       "43                          0/1:14,21:35:99:706,0,402  \n",
       "44                          0/1:28,25:53:99:592,0,910  \n",
       "45                            1/1:0,24:24:69:870,69,0  \n",
       "46                     0/1:137,131:268:99:4068,0,4525  \n",
       "\n",
       "[33 rows x 183 columns]"
      ]
     },
     "execution_count": 61,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Filtrar as variantes em um gene específico\n",
    "str_filter = multianno_df['Gene.refGene'] == \"BRCA2\"\n",
    "multianno_df[str_filter]"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 62,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>Func.refGene</th>\n",
       "      <th>Gene.refGene</th>\n",
       "      <th>GeneDetail.refGene</th>\n",
       "      <th>ExonicFunc.refGene</th>\n",
       "      <th>AAChange.refGene</th>\n",
       "      <th>...</th>\n",
       "      <th>VCF_CHR</th>\n",
       "      <th>VCF_POS</th>\n",
       "      <th>VCF_ID</th>\n",
       "      <th>VCF_REF</th>\n",
       "      <th>VCF_ALT</th>\n",
       "      <th>VCF_QUAL_2</th>\n",
       "      <th>VCF_FILTER</th>\n",
       "      <th>VCF_INFO</th>\n",
       "      <th>VCF_FORMAT</th>\n",
       "      <th>VCF_SAMPLE_NAME</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623861</td>\n",
       "      <td>89623861</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>splicing</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304717:exon1:c.154+1T&gt;-;NM_001304717:exo...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623860</td>\n",
       "      <td>rs71022512</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>234.80</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,6:7:18:272,18,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>89623901</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>exonic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>nonsynonymous SNV</td>\n",
       "      <td>PTEN:NM_001304717:exon2:c.194G&gt;C:p.C65S</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>rs2943772</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>440.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,14:14:36:469,36,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>89654121</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>rs139651072</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>216.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:10,8:18:99:245,0,289</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>5</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190</td>\n",
       "      <td>89654190</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190</td>\n",
       "      <td>rs181780682</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>29.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.714;AC=1;AF=0.5;AN=2;BaseQRankSum=0.36...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:5,2:7:58:58,0,172</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>6</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89711576</td>\n",
       "      <td>89711576</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89711576</td>\n",
       "      <td>rs141005791</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>96.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.21...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:5,4:9:99:125,0,133</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>7</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89718057</td>\n",
       "      <td>89718057</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89718057</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>759.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.324;AC=1;AF=0.5;AN=2;BaseQRankSum=0.83...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:11,23:34:99:788,0,283</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>8</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720548</td>\n",
       "      <td>89720548</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720548</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>1491.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.467;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.5...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:42,48:90:99:1520,0,1330</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>9</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720634</td>\n",
       "      <td>89720634</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720633</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>1805.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.601;DP=689;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:443,192:689:99:1843,0,6330</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>10</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720907</td>\n",
       "      <td>89720907</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720907</td>\n",
       "      <td>rs555895</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>10070.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.555;AC=1;AF=0.5;AN=2;BaseQRankSum=6.08...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:397,318:717:99:10099,0,11475</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>11</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89721094</td>\n",
       "      <td>89721094</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89721094</td>\n",
       "      <td>rs2736627</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>3289.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=92;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,92:92:99:3318,259,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>12</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725294</td>\n",
       "      <td>89725294</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304718:c.*65delT;NM_001304717:c.*65delT;...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89725293</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>9448.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.417;DP=2406;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1610,625:2406:99:9486,0,34365</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>13</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582</td>\n",
       "      <td>89725582</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304718:c.*353C&gt;T;NM_001304717:c.*353C&gt;T;...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582</td>\n",
       "      <td>rs181234898</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>201.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.526;AC=1;AF=0.5;AN=2;BaseQRankSum=0.31...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:10,9:19:99:230,0,266</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>68</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7576265</td>\n",
       "      <td>7576265</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7576264</td>\n",
       "      <td>.</td>\n",
       "      <td>TA</td>\n",
       "      <td>T</td>\n",
       "      <td>105.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.354;DP=29;Exce...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:20,9:29:99:143,0,380</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>69</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7576443</td>\n",
       "      <td>7576443</td>\n",
       "      <td>-</td>\n",
       "      <td>AA</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7576443</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>CAA</td>\n",
       "      <td>605.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-1.367;ClippingR...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:11,24:35:99:1:0.5:566,0,111:11,0,24,0:10,0...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>70</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7577679</td>\n",
       "      <td>7577679</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7577678</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>7065.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=1.437;DP=1700;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:805,734:1700:99:7103,0,11325</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>71</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7578115</td>\n",
       "      <td>7578115</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7578115</td>\n",
       "      <td>rs1625895</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>13711.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=362;Dels=0;Excess...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,362:362:99:13740,1074,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>72</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7578645</td>\n",
       "      <td>7578645</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>TP53</td>\n",
       "      <td>NM_001276697:c.-193G&gt;A;NM_001276698:c.-193G&gt;A;...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7578645</td>\n",
       "      <td>rs2909430</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>2001.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=62;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,61:62:99:2030,157,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>73</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7578712</td>\n",
       "      <td>7578715</td>\n",
       "      <td>TTTT</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>TP53</td>\n",
       "      <td>NM_001276697:c.-260_-263delAAAA;NM_001276698:c...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7578711</td>\n",
       "      <td>.</td>\n",
       "      <td>CTTTT</td>\n",
       "      <td>C</td>\n",
       "      <td>78271.70</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.215;DP=2426;E...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/.:152,1138:2426:99:78309,15975,16093</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>74</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7578712</td>\n",
       "      <td>7578714</td>\n",
       "      <td>TTT</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>TP53</td>\n",
       "      <td>NM_001276697:c.-260_-262delAAA;NM_001276698:c....</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7578711</td>\n",
       "      <td>.</td>\n",
       "      <td>CTTT</td>\n",
       "      <td>C</td>\n",
       "      <td>78271.70</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.215;DP=2426;E...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>./1:152,971:2426:99:78309,16727,13403</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>75</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7578837</td>\n",
       "      <td>7578837</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7578837</td>\n",
       "      <td>rs1794287</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>61525.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=1680;Dels=0;Exces...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,1672:1679:99:61554,4803,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>76</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7579472</td>\n",
       "      <td>7579472</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>exonic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>nonsynonymous SNV</td>\n",
       "      <td>TP53:NM_001126118:exon3:c.98C&gt;G:p.P33R,TP53:NM...</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7579472</td>\n",
       "      <td>rs1042522</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>74058.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=0.982;AC=2;AF=1;AN=2;BaseQRankSum=11.225...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:57,3146:3215:99:74087,5357,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>77</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7579644</td>\n",
       "      <td>7579659</td>\n",
       "      <td>CCCCAGCCCTCCAGGT</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7579643</td>\n",
       "      <td>rs146534833</td>\n",
       "      <td>CCCCCAGCCCTCCAGGT</td>\n",
       "      <td>C</td>\n",
       "      <td>122210.00</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=2;AF=1;AN=2;DB;DP=1405;ExcessHet=3.0103;FS=...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,924:1405:99:122247,2780,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>78</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7579801</td>\n",
       "      <td>7579801</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>TP53</td>\n",
       "      <td>NM_001126118:c.-232C&gt;G</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7579801</td>\n",
       "      <td>rs1642785</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>9509.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=0.651;AC=2;AF=1;AN=2;BaseQRankSum=16.02;...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:203,378:582:99:9538,522,0</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>23 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr     Start       End               Ref Alt Func.refGene Gene.refGene  \\\n",
       "2   chr10  89623861  89623861                 T   -     splicing         PTEN   \n",
       "3   chr10  89623901  89623901                 G   C       exonic         PTEN   \n",
       "4   chr10  89654121  89654121                 T   C     intronic         PTEN   \n",
       "5   chr10  89654190  89654190                 G   A     intronic         PTEN   \n",
       "6   chr10  89711576  89711576                 C   T     intronic         PTEN   \n",
       "7   chr10  89718057  89718057                 A   G     intronic         PTEN   \n",
       "8   chr10  89720548  89720548                 A   G     intronic         PTEN   \n",
       "9   chr10  89720634  89720634                 T   -     intronic         PTEN   \n",
       "10  chr10  89720907  89720907                 T   G     intronic         PTEN   \n",
       "11  chr10  89721094  89721094                 C   T     intronic         PTEN   \n",
       "12  chr10  89725294  89725294                 T   -         UTR3         PTEN   \n",
       "13  chr10  89725582  89725582                 C   T         UTR3         PTEN   \n",
       "68  chr17   7576265   7576265                 A   -     intronic         TP53   \n",
       "69  chr17   7576443   7576443                 -  AA     intronic         TP53   \n",
       "70  chr17   7577679   7577679                 T   -     intronic         TP53   \n",
       "71  chr17   7578115   7578115                 T   C     intronic         TP53   \n",
       "72  chr17   7578645   7578645                 C   T         UTR5         TP53   \n",
       "73  chr17   7578712   7578715              TTTT   -         UTR5         TP53   \n",
       "74  chr17   7578712   7578714               TTT   -         UTR5         TP53   \n",
       "75  chr17   7578837   7578837                 A   G     intronic         TP53   \n",
       "76  chr17   7579472   7579472                 G   C       exonic         TP53   \n",
       "77  chr17   7579644   7579659  CCCCAGCCCTCCAGGT   -     intronic         TP53   \n",
       "78  chr17   7579801   7579801                 G   C         UTR5         TP53   \n",
       "\n",
       "                                   GeneDetail.refGene ExonicFunc.refGene  \\\n",
       "2   NM_001304717:exon1:c.154+1T>-;NM_001304717:exo...                  .   \n",
       "3                                                   .  nonsynonymous SNV   \n",
       "4                                                   .                  .   \n",
       "5                                                   .                  .   \n",
       "6                                                   .                  .   \n",
       "7                                                   .                  .   \n",
       "8                                                   .                  .   \n",
       "9                                                   .                  .   \n",
       "10                                                  .                  .   \n",
       "11                                                  .                  .   \n",
       "12  NM_001304718:c.*65delT;NM_001304717:c.*65delT;...                  .   \n",
       "13  NM_001304718:c.*353C>T;NM_001304717:c.*353C>T;...                  .   \n",
       "68                                                  .                  .   \n",
       "69                                                  .                  .   \n",
       "70                                                  .                  .   \n",
       "71                                                  .                  .   \n",
       "72  NM_001276697:c.-193G>A;NM_001276698:c.-193G>A;...                  .   \n",
       "73  NM_001276697:c.-260_-263delAAAA;NM_001276698:c...                  .   \n",
       "74  NM_001276697:c.-260_-262delAAA;NM_001276698:c....                  .   \n",
       "75                                                  .                  .   \n",
       "76                                                  .  nonsynonymous SNV   \n",
       "77                                                  .                  .   \n",
       "78                             NM_001126118:c.-232C>G                  .   \n",
       "\n",
       "                                     AAChange.refGene  ... VCF_CHR   VCF_POS  \\\n",
       "2                                                   .  ...   chr10  89623860   \n",
       "3             PTEN:NM_001304717:exon2:c.194G>C:p.C65S  ...   chr10  89623901   \n",
       "4                                                   .  ...   chr10  89654121   \n",
       "5                                                   .  ...   chr10  89654190   \n",
       "6                                                   .  ...   chr10  89711576   \n",
       "7                                                   .  ...   chr10  89718057   \n",
       "8                                                   .  ...   chr10  89720548   \n",
       "9                                                   .  ...   chr10  89720633   \n",
       "10                                                  .  ...   chr10  89720907   \n",
       "11                                                  .  ...   chr10  89721094   \n",
       "12                                                  .  ...   chr10  89725293   \n",
       "13                                                  .  ...   chr10  89725582   \n",
       "68                                                  .  ...   chr17   7576264   \n",
       "69                                                  .  ...   chr17   7576443   \n",
       "70                                                  .  ...   chr17   7577678   \n",
       "71                                                  .  ...   chr17   7578115   \n",
       "72                                                  .  ...   chr17   7578645   \n",
       "73                                                  .  ...   chr17   7578711   \n",
       "74                                                  .  ...   chr17   7578711   \n",
       "75                                                  .  ...   chr17   7578837   \n",
       "76  TP53:NM_001126118:exon3:c.98C>G:p.P33R,TP53:NM...  ...   chr17   7579472   \n",
       "77                                                  .  ...   chr17   7579643   \n",
       "78                                                  .  ...   chr17   7579801   \n",
       "\n",
       "         VCF_ID            VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "2    rs71022512                 CT       C     234.80          .   \n",
       "3     rs2943772                  G       C     440.77          .   \n",
       "4   rs139651072                  T       C     216.77          .   \n",
       "5   rs181780682                  G       A      29.77          .   \n",
       "6   rs141005791                  C       T      96.77          .   \n",
       "7             .                  A       G     759.77          .   \n",
       "8             .                  A       G    1491.77          .   \n",
       "9             .                 CT       C    1805.73          .   \n",
       "10     rs555895                  T       G   10070.80          .   \n",
       "11    rs2736627                  C       T    3289.77          .   \n",
       "12            .                 CT       C    9448.73          .   \n",
       "13  rs181234898                  C       T     201.77          .   \n",
       "68            .                 TA       T     105.73          .   \n",
       "69            .                  C     CAA     605.73          .   \n",
       "70            .                 CT       C    7065.73          .   \n",
       "71    rs1625895                  T       C   13711.80          .   \n",
       "72    rs2909430                  C       T    2001.77          .   \n",
       "73            .              CTTTT       C   78271.70          .   \n",
       "74            .               CTTT       C   78271.70          .   \n",
       "75    rs1794287                  A       G   61525.80          .   \n",
       "76    rs1042522                  G       C   74058.80          .   \n",
       "77  rs146534833  CCCCCAGCCCTCCAGGT       C  122210.00          .   \n",
       "78    rs1642785                  G       C    9509.77          .   \n",
       "\n",
       "                                             VCF_INFO  \\\n",
       "2   AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...   \n",
       "3   ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...   \n",
       "4   ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...   \n",
       "5   ABHet=0.714;AC=1;AF=0.5;AN=2;BaseQRankSum=0.36...   \n",
       "6   ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.21...   \n",
       "7   ABHet=0.324;AC=1;AF=0.5;AN=2;BaseQRankSum=0.83...   \n",
       "8   ABHet=0.467;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.5...   \n",
       "9   AC=1;AF=0.5;AN=2;BaseQRankSum=-0.601;DP=689;Ex...   \n",
       "10  ABHet=0.555;AC=1;AF=0.5;AN=2;BaseQRankSum=6.08...   \n",
       "11  ABHom=1;AC=2;AF=1;AN=2;DB;DP=92;Dels=0;ExcessH...   \n",
       "12  AC=1;AF=0.5;AN=2;BaseQRankSum=0.417;DP=2406;Ex...   \n",
       "13  ABHet=0.526;AC=1;AF=0.5;AN=2;BaseQRankSum=0.31...   \n",
       "68  AC=1;AF=0.5;AN=2;BaseQRankSum=0.354;DP=29;Exce...   \n",
       "69  AC=1;AF=0.5;AN=2;BaseQRankSum=-1.367;ClippingR...   \n",
       "70  AC=1;AF=0.5;AN=2;BaseQRankSum=1.437;DP=1700;Ex...   \n",
       "71  ABHom=1;AC=2;AF=1;AN=2;DB;DP=362;Dels=0;Excess...   \n",
       "72  ABHom=1;AC=2;AF=1;AN=2;DB;DP=62;Dels=0;ExcessH...   \n",
       "73  AC=1;AF=0.5;AN=2;BaseQRankSum=-0.215;DP=2426;E...   \n",
       "74  AC=1;AF=0.5;AN=2;BaseQRankSum=-0.215;DP=2426;E...   \n",
       "75  ABHom=1;AC=2;AF=1;AN=2;DB;DP=1680;Dels=0;Exces...   \n",
       "76  ABHom=0.982;AC=2;AF=1;AN=2;BaseQRankSum=11.225...   \n",
       "77  AC=2;AF=1;AN=2;DB;DP=1405;ExcessHet=3.0103;FS=...   \n",
       "78  ABHom=0.651;AC=2;AF=1;AN=2;BaseQRankSum=16.02;...   \n",
       "\n",
       "                             VCF_FORMAT  \\\n",
       "2                        GT:AD:DP:GQ:PL   \n",
       "3                        GT:AD:DP:GQ:PL   \n",
       "4                        GT:AD:DP:GQ:PL   \n",
       "5                        GT:AD:DP:GQ:PL   \n",
       "6                        GT:AD:DP:GQ:PL   \n",
       "7                        GT:AD:DP:GQ:PL   \n",
       "8                        GT:AD:DP:GQ:PL   \n",
       "9                        GT:AD:DP:GQ:PL   \n",
       "10                       GT:AD:DP:GQ:PL   \n",
       "11                       GT:AD:DP:GQ:PL   \n",
       "12                       GT:AD:DP:GQ:PL   \n",
       "13                       GT:AD:DP:GQ:PL   \n",
       "68                       GT:AD:DP:GQ:PL   \n",
       "69  GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "70                       GT:AD:DP:GQ:PL   \n",
       "71                       GT:AD:DP:GQ:PL   \n",
       "72                       GT:AD:DP:GQ:PL   \n",
       "73                       GT:AD:DP:GQ:PL   \n",
       "74                       GT:AD:DP:GQ:PL   \n",
       "75                       GT:AD:DP:GQ:PL   \n",
       "76                       GT:AD:DP:GQ:PL   \n",
       "77                       GT:AD:DP:GQ:PL   \n",
       "78                       GT:AD:DP:GQ:PL   \n",
       "\n",
       "                                      VCF_SAMPLE_NAME  \n",
       "2                               1/1:0,6:7:18:272,18,0  \n",
       "3                             1/1:0,14:14:36:469,36,0  \n",
       "4                            0/1:10,8:18:99:245,0,289  \n",
       "5                               0/1:5,2:7:58:58,0,172  \n",
       "6                              0/1:5,4:9:99:125,0,133  \n",
       "7                           0/1:11,23:34:99:788,0,283  \n",
       "8                         0/1:42,48:90:99:1520,0,1330  \n",
       "9                      0/1:443,192:689:99:1843,0,6330  \n",
       "10                   0/1:397,318:717:99:10099,0,11475  \n",
       "11                          1/1:0,92:92:99:3318,259,0  \n",
       "12                  0/1:1610,625:2406:99:9486,0,34365  \n",
       "13                           0/1:10,9:19:99:230,0,266  \n",
       "68                           0/1:20,9:29:99:143,0,380  \n",
       "69  0/1:11,24:35:99:1:0.5:566,0,111:11,0,24,0:10,0...  \n",
       "70                   0/1:805,734:1700:99:7103,0,11325  \n",
       "71                      1/1:0,362:362:99:13740,1074,0  \n",
       "72                          1/1:0,61:62:99:2030,157,0  \n",
       "73             1/.:152,1138:2426:99:78309,15975,16093  \n",
       "74              ./1:152,971:2426:99:78309,16727,13403  \n",
       "75                    1/1:0,1672:1679:99:61554,4803,0  \n",
       "76                   1/1:57,3146:3215:99:74087,5357,0  \n",
       "77                    1/1:0,924:1405:99:122247,2780,0  \n",
       "78                      1/1:203,378:582:99:9538,522,0  \n",
       "\n",
       "[23 rows x 183 columns]"
      ]
     },
     "execution_count": 62,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Filtrar variantes em uma lista de genes\n",
    "gene_list = [\"PTEN\", \"TP53\"]\n",
    "str_filter = multianno_df[\"Gene.refGene\"].isin(gene_list)\n",
    "multianno_df[str_filter]"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* OUXEN, mas faltou variante que tinha como gene `TP53;WRAP53'` e agora?\n",
    "\n",
    "> Pesquise por [`pandas parcial match values in list`](https://stackoverflow.com/questions/38333582/search-for-a-partial-string-match-in-a-data-frame-column-from-a-list-pandas) no google xD"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 63,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "'PTEN|TP53'"
      ]
     },
     "execution_count": 63,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "pat = \"|\".join(gene_list)\n",
    "pat"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 64,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
       "    .dataframe tbody tr th:only-of-type {\n",
       "        vertical-align: middle;\n",
       "    }\n",
       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>Func.refGene</th>\n",
       "      <th>Gene.refGene</th>\n",
       "      <th>GeneDetail.refGene</th>\n",
       "      <th>ExonicFunc.refGene</th>\n",
       "      <th>AAChange.refGene</th>\n",
       "      <th>...</th>\n",
       "      <th>VCF_CHR</th>\n",
       "      <th>VCF_POS</th>\n",
       "      <th>VCF_ID</th>\n",
       "      <th>VCF_REF</th>\n",
       "      <th>VCF_ALT</th>\n",
       "      <th>VCF_QUAL_2</th>\n",
       "      <th>VCF_FILTER</th>\n",
       "      <th>VCF_INFO</th>\n",
       "      <th>VCF_FORMAT</th>\n",
       "      <th>VCF_SAMPLE_NAME</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623861</td>\n",
       "      <td>89623861</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>splicing</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304717:exon1:c.154+1T&gt;-;NM_001304717:exo...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623860</td>\n",
       "      <td>rs71022512</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>234.80</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,6:7:18:272,18,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>89623901</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>exonic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>nonsynonymous SNV</td>\n",
       "      <td>PTEN:NM_001304717:exon2:c.194G&gt;C:p.C65S</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901</td>\n",
       "      <td>rs2943772</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>440.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,14:14:36:469,36,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>89654121</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654121</td>\n",
       "      <td>rs139651072</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>216.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:10,8:18:99:245,0,289</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>5</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190</td>\n",
       "      <td>89654190</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190</td>\n",
       "      <td>rs181780682</td>\n",
       "      <td>G</td>\n",
       "      <td>A</td>\n",
       "      <td>29.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.714;AC=1;AF=0.5;AN=2;BaseQRankSum=0.36...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:5,2:7:58:58,0,172</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>6</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89711576</td>\n",
       "      <td>89711576</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89711576</td>\n",
       "      <td>rs141005791</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>96.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.21...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:5,4:9:99:125,0,133</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>7</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89718057</td>\n",
       "      <td>89718057</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89718057</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>759.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.324;AC=1;AF=0.5;AN=2;BaseQRankSum=0.83...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:11,23:34:99:788,0,283</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>8</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720548</td>\n",
       "      <td>89720548</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720548</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>1491.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.467;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.5...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:42,48:90:99:1520,0,1330</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>9</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720634</td>\n",
       "      <td>89720634</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720633</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>1805.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.601;DP=689;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:443,192:689:99:1843,0,6330</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>10</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720907</td>\n",
       "      <td>89720907</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89720907</td>\n",
       "      <td>rs555895</td>\n",
       "      <td>T</td>\n",
       "      <td>G</td>\n",
       "      <td>10070.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.555;AC=1;AF=0.5;AN=2;BaseQRankSum=6.08...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:397,318:717:99:10099,0,11475</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>11</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89721094</td>\n",
       "      <td>89721094</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>intronic</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89721094</td>\n",
       "      <td>rs2736627</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>3289.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=92;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,92:92:99:3318,259,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>12</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725294</td>\n",
       "      <td>89725294</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304718:c.*65delT;NM_001304717:c.*65delT;...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89725293</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>9448.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.417;DP=2406;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:1610,625:2406:99:9486,0,34365</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>13</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582</td>\n",
       "      <td>89725582</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>PTEN</td>\n",
       "      <td>NM_001304718:c.*353C&gt;T;NM_001304717:c.*353C&gt;T;...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582</td>\n",
       "      <td>rs181234898</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>201.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.526;AC=1;AF=0.5;AN=2;BaseQRankSum=0.31...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:10,9:19:99:230,0,266</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>68</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7576265</td>\n",
       "      <td>7576265</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7576264</td>\n",
       "      <td>.</td>\n",
       "      <td>TA</td>\n",
       "      <td>T</td>\n",
       "      <td>105.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.354;DP=29;Exce...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:20,9:29:99:143,0,380</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>69</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7576443</td>\n",
       "      <td>7576443</td>\n",
       "      <td>-</td>\n",
       "      <td>AA</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7576443</td>\n",
       "      <td>.</td>\n",
       "      <td>C</td>\n",
       "      <td>CAA</td>\n",
       "      <td>605.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-1.367;ClippingR...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:11,24:35:99:1:0.5:566,0,111:11,0,24,0:10,0...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>70</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7577679</td>\n",
       "      <td>7577679</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7577678</td>\n",
       "      <td>.</td>\n",
       "      <td>CT</td>\n",
       "      <td>C</td>\n",
       "      <td>7065.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=1.437;DP=1700;Ex...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:805,734:1700:99:7103,0,11325</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>71</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7578115</td>\n",
       "      <td>7578115</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7578115</td>\n",
       "      <td>rs1625895</td>\n",
       "      <td>T</td>\n",
       "      <td>C</td>\n",
       "      <td>13711.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=362;Dels=0;Excess...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,362:362:99:13740,1074,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>72</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7578645</td>\n",
       "      <td>7578645</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>TP53</td>\n",
       "      <td>NM_001276697:c.-193G&gt;A;NM_001276698:c.-193G&gt;A;...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7578645</td>\n",
       "      <td>rs2909430</td>\n",
       "      <td>C</td>\n",
       "      <td>T</td>\n",
       "      <td>2001.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=62;Dels=0;ExcessH...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,61:62:99:2030,157,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>73</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7578712</td>\n",
       "      <td>7578715</td>\n",
       "      <td>TTTT</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>TP53</td>\n",
       "      <td>NM_001276697:c.-260_-263delAAAA;NM_001276698:c...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7578711</td>\n",
       "      <td>.</td>\n",
       "      <td>CTTTT</td>\n",
       "      <td>C</td>\n",
       "      <td>78271.70</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.215;DP=2426;E...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/.:152,1138:2426:99:78309,15975,16093</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>74</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7578712</td>\n",
       "      <td>7578714</td>\n",
       "      <td>TTT</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>TP53</td>\n",
       "      <td>NM_001276697:c.-260_-262delAAA;NM_001276698:c....</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7578711</td>\n",
       "      <td>.</td>\n",
       "      <td>CTTT</td>\n",
       "      <td>C</td>\n",
       "      <td>78271.70</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.215;DP=2426;E...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>./1:152,971:2426:99:78309,16727,13403</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>75</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7578837</td>\n",
       "      <td>7578837</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7578837</td>\n",
       "      <td>rs1794287</td>\n",
       "      <td>A</td>\n",
       "      <td>G</td>\n",
       "      <td>61525.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=1;AC=2;AF=1;AN=2;DB;DP=1680;Dels=0;Exces...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,1672:1679:99:61554,4803,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>76</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7579472</td>\n",
       "      <td>7579472</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>exonic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>nonsynonymous SNV</td>\n",
       "      <td>TP53:NM_001126118:exon3:c.98C&gt;G:p.P33R,TP53:NM...</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7579472</td>\n",
       "      <td>rs1042522</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>74058.80</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=0.982;AC=2;AF=1;AN=2;BaseQRankSum=11.225...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:57,3146:3215:99:74087,5357,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>77</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7579644</td>\n",
       "      <td>7579659</td>\n",
       "      <td>CCCCAGCCCTCCAGGT</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7579643</td>\n",
       "      <td>rs146534833</td>\n",
       "      <td>CCCCCAGCCCTCCAGGT</td>\n",
       "      <td>C</td>\n",
       "      <td>122210.00</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=2;AF=1;AN=2;DB;DP=1405;ExcessHet=3.0103;FS=...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:0,924:1405:99:122247,2780,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>78</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7579801</td>\n",
       "      <td>7579801</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>UTR5</td>\n",
       "      <td>TP53</td>\n",
       "      <td>NM_001126118:c.-232C&gt;G</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7579801</td>\n",
       "      <td>rs1642785</td>\n",
       "      <td>G</td>\n",
       "      <td>C</td>\n",
       "      <td>9509.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHom=0.651;AC=2;AF=1;AN=2;BaseQRankSum=16.02;...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>1/1:203,378:582:99:9538,522,0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>79</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7590593</td>\n",
       "      <td>7590593</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53;WRAP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7590593</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>87.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.749;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.3...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:188,63:251:99:116,0,652</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>80</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7590601</td>\n",
       "      <td>7590601</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53;WRAP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7590601</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>339.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.585;AC=1;AF=0.5;AN=2;BaseQRankSum=-2.0...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:172,122:294:99:368,0,1128</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>81</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7590607</td>\n",
       "      <td>7590607</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>intronic</td>\n",
       "      <td>TP53;WRAP53</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>7590607</td>\n",
       "      <td>.</td>\n",
       "      <td>A</td>\n",
       "      <td>C</td>\n",
       "      <td>41.77</td>\n",
       "      <td>.</td>\n",
       "      <td>ABHet=0.704;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.0...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:226,95:321:70:70,0,2914</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>26 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr     Start       End               Ref Alt Func.refGene Gene.refGene  \\\n",
       "2   chr10  89623861  89623861                 T   -     splicing         PTEN   \n",
       "3   chr10  89623901  89623901                 G   C       exonic         PTEN   \n",
       "4   chr10  89654121  89654121                 T   C     intronic         PTEN   \n",
       "5   chr10  89654190  89654190                 G   A     intronic         PTEN   \n",
       "6   chr10  89711576  89711576                 C   T     intronic         PTEN   \n",
       "7   chr10  89718057  89718057                 A   G     intronic         PTEN   \n",
       "8   chr10  89720548  89720548                 A   G     intronic         PTEN   \n",
       "9   chr10  89720634  89720634                 T   -     intronic         PTEN   \n",
       "10  chr10  89720907  89720907                 T   G     intronic         PTEN   \n",
       "11  chr10  89721094  89721094                 C   T     intronic         PTEN   \n",
       "12  chr10  89725294  89725294                 T   -         UTR3         PTEN   \n",
       "13  chr10  89725582  89725582                 C   T         UTR3         PTEN   \n",
       "68  chr17   7576265   7576265                 A   -     intronic         TP53   \n",
       "69  chr17   7576443   7576443                 -  AA     intronic         TP53   \n",
       "70  chr17   7577679   7577679                 T   -     intronic         TP53   \n",
       "71  chr17   7578115   7578115                 T   C     intronic         TP53   \n",
       "72  chr17   7578645   7578645                 C   T         UTR5         TP53   \n",
       "73  chr17   7578712   7578715              TTTT   -         UTR5         TP53   \n",
       "74  chr17   7578712   7578714               TTT   -         UTR5         TP53   \n",
       "75  chr17   7578837   7578837                 A   G     intronic         TP53   \n",
       "76  chr17   7579472   7579472                 G   C       exonic         TP53   \n",
       "77  chr17   7579644   7579659  CCCCAGCCCTCCAGGT   -     intronic         TP53   \n",
       "78  chr17   7579801   7579801                 G   C         UTR5         TP53   \n",
       "79  chr17   7590593   7590593                 A   C     intronic  TP53;WRAP53   \n",
       "80  chr17   7590601   7590601                 A   C     intronic  TP53;WRAP53   \n",
       "81  chr17   7590607   7590607                 A   C     intronic  TP53;WRAP53   \n",
       "\n",
       "                                   GeneDetail.refGene ExonicFunc.refGene  \\\n",
       "2   NM_001304717:exon1:c.154+1T>-;NM_001304717:exo...                  .   \n",
       "3                                                   .  nonsynonymous SNV   \n",
       "4                                                   .                  .   \n",
       "5                                                   .                  .   \n",
       "6                                                   .                  .   \n",
       "7                                                   .                  .   \n",
       "8                                                   .                  .   \n",
       "9                                                   .                  .   \n",
       "10                                                  .                  .   \n",
       "11                                                  .                  .   \n",
       "12  NM_001304718:c.*65delT;NM_001304717:c.*65delT;...                  .   \n",
       "13  NM_001304718:c.*353C>T;NM_001304717:c.*353C>T;...                  .   \n",
       "68                                                  .                  .   \n",
       "69                                                  .                  .   \n",
       "70                                                  .                  .   \n",
       "71                                                  .                  .   \n",
       "72  NM_001276697:c.-193G>A;NM_001276698:c.-193G>A;...                  .   \n",
       "73  NM_001276697:c.-260_-263delAAAA;NM_001276698:c...                  .   \n",
       "74  NM_001276697:c.-260_-262delAAA;NM_001276698:c....                  .   \n",
       "75                                                  .                  .   \n",
       "76                                                  .  nonsynonymous SNV   \n",
       "77                                                  .                  .   \n",
       "78                             NM_001126118:c.-232C>G                  .   \n",
       "79                                                  .                  .   \n",
       "80                                                  .                  .   \n",
       "81                                                  .                  .   \n",
       "\n",
       "                                     AAChange.refGene  ... VCF_CHR   VCF_POS  \\\n",
       "2                                                   .  ...   chr10  89623860   \n",
       "3             PTEN:NM_001304717:exon2:c.194G>C:p.C65S  ...   chr10  89623901   \n",
       "4                                                   .  ...   chr10  89654121   \n",
       "5                                                   .  ...   chr10  89654190   \n",
       "6                                                   .  ...   chr10  89711576   \n",
       "7                                                   .  ...   chr10  89718057   \n",
       "8                                                   .  ...   chr10  89720548   \n",
       "9                                                   .  ...   chr10  89720633   \n",
       "10                                                  .  ...   chr10  89720907   \n",
       "11                                                  .  ...   chr10  89721094   \n",
       "12                                                  .  ...   chr10  89725293   \n",
       "13                                                  .  ...   chr10  89725582   \n",
       "68                                                  .  ...   chr17   7576264   \n",
       "69                                                  .  ...   chr17   7576443   \n",
       "70                                                  .  ...   chr17   7577678   \n",
       "71                                                  .  ...   chr17   7578115   \n",
       "72                                                  .  ...   chr17   7578645   \n",
       "73                                                  .  ...   chr17   7578711   \n",
       "74                                                  .  ...   chr17   7578711   \n",
       "75                                                  .  ...   chr17   7578837   \n",
       "76  TP53:NM_001126118:exon3:c.98C>G:p.P33R,TP53:NM...  ...   chr17   7579472   \n",
       "77                                                  .  ...   chr17   7579643   \n",
       "78                                                  .  ...   chr17   7579801   \n",
       "79                                                  .  ...   chr17   7590593   \n",
       "80                                                  .  ...   chr17   7590601   \n",
       "81                                                  .  ...   chr17   7590607   \n",
       "\n",
       "         VCF_ID            VCF_REF VCF_ALT VCF_QUAL_2 VCF_FILTER  \\\n",
       "2    rs71022512                 CT       C     234.80          .   \n",
       "3     rs2943772                  G       C     440.77          .   \n",
       "4   rs139651072                  T       C     216.77          .   \n",
       "5   rs181780682                  G       A      29.77          .   \n",
       "6   rs141005791                  C       T      96.77          .   \n",
       "7             .                  A       G     759.77          .   \n",
       "8             .                  A       G    1491.77          .   \n",
       "9             .                 CT       C    1805.73          .   \n",
       "10     rs555895                  T       G   10070.80          .   \n",
       "11    rs2736627                  C       T    3289.77          .   \n",
       "12            .                 CT       C    9448.73          .   \n",
       "13  rs181234898                  C       T     201.77          .   \n",
       "68            .                 TA       T     105.73          .   \n",
       "69            .                  C     CAA     605.73          .   \n",
       "70            .                 CT       C    7065.73          .   \n",
       "71    rs1625895                  T       C   13711.80          .   \n",
       "72    rs2909430                  C       T    2001.77          .   \n",
       "73            .              CTTTT       C   78271.70          .   \n",
       "74            .               CTTT       C   78271.70          .   \n",
       "75    rs1794287                  A       G   61525.80          .   \n",
       "76    rs1042522                  G       C   74058.80          .   \n",
       "77  rs146534833  CCCCCAGCCCTCCAGGT       C  122210.00          .   \n",
       "78    rs1642785                  G       C    9509.77          .   \n",
       "79            .                  A       C      87.77          .   \n",
       "80            .                  A       C     339.77          .   \n",
       "81            .                  A       C      41.77          .   \n",
       "\n",
       "                                             VCF_INFO  \\\n",
       "2   AC=2;AF=1;AN=2;DB;DP=7;ExcessHet=3.0103;FS=0;M...   \n",
       "3   ABHom=1;AC=2;AF=1;AN=2;DB;DP=14;Dels=0;ExcessH...   \n",
       "4   ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.15...   \n",
       "5   ABHet=0.714;AC=1;AF=0.5;AN=2;BaseQRankSum=0.36...   \n",
       "6   ABHet=0.556;AC=1;AF=0.5;AN=2;BaseQRankSum=0.21...   \n",
       "7   ABHet=0.324;AC=1;AF=0.5;AN=2;BaseQRankSum=0.83...   \n",
       "8   ABHet=0.467;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.5...   \n",
       "9   AC=1;AF=0.5;AN=2;BaseQRankSum=-0.601;DP=689;Ex...   \n",
       "10  ABHet=0.555;AC=1;AF=0.5;AN=2;BaseQRankSum=6.08...   \n",
       "11  ABHom=1;AC=2;AF=1;AN=2;DB;DP=92;Dels=0;ExcessH...   \n",
       "12  AC=1;AF=0.5;AN=2;BaseQRankSum=0.417;DP=2406;Ex...   \n",
       "13  ABHet=0.526;AC=1;AF=0.5;AN=2;BaseQRankSum=0.31...   \n",
       "68  AC=1;AF=0.5;AN=2;BaseQRankSum=0.354;DP=29;Exce...   \n",
       "69  AC=1;AF=0.5;AN=2;BaseQRankSum=-1.367;ClippingR...   \n",
       "70  AC=1;AF=0.5;AN=2;BaseQRankSum=1.437;DP=1700;Ex...   \n",
       "71  ABHom=1;AC=2;AF=1;AN=2;DB;DP=362;Dels=0;Excess...   \n",
       "72  ABHom=1;AC=2;AF=1;AN=2;DB;DP=62;Dels=0;ExcessH...   \n",
       "73  AC=1;AF=0.5;AN=2;BaseQRankSum=-0.215;DP=2426;E...   \n",
       "74  AC=1;AF=0.5;AN=2;BaseQRankSum=-0.215;DP=2426;E...   \n",
       "75  ABHom=1;AC=2;AF=1;AN=2;DB;DP=1680;Dels=0;Exces...   \n",
       "76  ABHom=0.982;AC=2;AF=1;AN=2;BaseQRankSum=11.225...   \n",
       "77  AC=2;AF=1;AN=2;DB;DP=1405;ExcessHet=3.0103;FS=...   \n",
       "78  ABHom=0.651;AC=2;AF=1;AN=2;BaseQRankSum=16.02;...   \n",
       "79  ABHet=0.749;AC=1;AF=0.5;AN=2;BaseQRankSum=-0.3...   \n",
       "80  ABHet=0.585;AC=1;AF=0.5;AN=2;BaseQRankSum=-2.0...   \n",
       "81  ABHet=0.704;AC=1;AF=0.5;AN=2;BaseQRankSum=-5.0...   \n",
       "\n",
       "                             VCF_FORMAT  \\\n",
       "2                        GT:AD:DP:GQ:PL   \n",
       "3                        GT:AD:DP:GQ:PL   \n",
       "4                        GT:AD:DP:GQ:PL   \n",
       "5                        GT:AD:DP:GQ:PL   \n",
       "6                        GT:AD:DP:GQ:PL   \n",
       "7                        GT:AD:DP:GQ:PL   \n",
       "8                        GT:AD:DP:GQ:PL   \n",
       "9                        GT:AD:DP:GQ:PL   \n",
       "10                       GT:AD:DP:GQ:PL   \n",
       "11                       GT:AD:DP:GQ:PL   \n",
       "12                       GT:AD:DP:GQ:PL   \n",
       "13                       GT:AD:DP:GQ:PL   \n",
       "68                       GT:AD:DP:GQ:PL   \n",
       "69  GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "70                       GT:AD:DP:GQ:PL   \n",
       "71                       GT:AD:DP:GQ:PL   \n",
       "72                       GT:AD:DP:GQ:PL   \n",
       "73                       GT:AD:DP:GQ:PL   \n",
       "74                       GT:AD:DP:GQ:PL   \n",
       "75                       GT:AD:DP:GQ:PL   \n",
       "76                       GT:AD:DP:GQ:PL   \n",
       "77                       GT:AD:DP:GQ:PL   \n",
       "78                       GT:AD:DP:GQ:PL   \n",
       "79                       GT:AD:DP:GQ:PL   \n",
       "80                       GT:AD:DP:GQ:PL   \n",
       "81                       GT:AD:DP:GQ:PL   \n",
       "\n",
       "                                      VCF_SAMPLE_NAME  \n",
       "2                               1/1:0,6:7:18:272,18,0  \n",
       "3                             1/1:0,14:14:36:469,36,0  \n",
       "4                            0/1:10,8:18:99:245,0,289  \n",
       "5                               0/1:5,2:7:58:58,0,172  \n",
       "6                              0/1:5,4:9:99:125,0,133  \n",
       "7                           0/1:11,23:34:99:788,0,283  \n",
       "8                         0/1:42,48:90:99:1520,0,1330  \n",
       "9                      0/1:443,192:689:99:1843,0,6330  \n",
       "10                   0/1:397,318:717:99:10099,0,11475  \n",
       "11                          1/1:0,92:92:99:3318,259,0  \n",
       "12                  0/1:1610,625:2406:99:9486,0,34365  \n",
       "13                           0/1:10,9:19:99:230,0,266  \n",
       "68                           0/1:20,9:29:99:143,0,380  \n",
       "69  0/1:11,24:35:99:1:0.5:566,0,111:11,0,24,0:10,0...  \n",
       "70                   0/1:805,734:1700:99:7103,0,11325  \n",
       "71                      1/1:0,362:362:99:13740,1074,0  \n",
       "72                          1/1:0,61:62:99:2030,157,0  \n",
       "73             1/.:152,1138:2426:99:78309,15975,16093  \n",
       "74              ./1:152,971:2426:99:78309,16727,13403  \n",
       "75                    1/1:0,1672:1679:99:61554,4803,0  \n",
       "76                   1/1:57,3146:3215:99:74087,5357,0  \n",
       "77                    1/1:0,924:1405:99:122247,2780,0  \n",
       "78                      1/1:203,378:582:99:9538,522,0  \n",
       "79                        0/1:188,63:251:99:116,0,652  \n",
       "80                      0/1:172,122:294:99:368,0,1128  \n",
       "81                        0/1:226,95:321:70:70,0,2914  \n",
       "\n",
       "[26 rows x 183 columns]"
      ]
     },
     "execution_count": 64,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "str_filter = multianno_df[\"Gene.refGene\"].str.contains(pat)\n",
    "multianno_df[str_filter]"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Esses filtros por coluna podem ser feitos usando a função `query()`"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 65,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
       "<div>\n",
       "<style scoped>\n",
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       "\n",
       "    .dataframe tbody tr th {\n",
       "        vertical-align: top;\n",
       "    }\n",
       "\n",
       "    .dataframe thead th {\n",
       "        text-align: right;\n",
       "    }\n",
       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "      <th>Ref</th>\n",
       "      <th>Alt</th>\n",
       "      <th>Func.refGene</th>\n",
       "      <th>Gene.refGene</th>\n",
       "      <th>GeneDetail.refGene</th>\n",
       "      <th>ExonicFunc.refGene</th>\n",
       "      <th>AAChange.refGene</th>\n",
       "      <th>...</th>\n",
       "      <th>VCF_CHR</th>\n",
       "      <th>VCF_POS</th>\n",
       "      <th>VCF_ID</th>\n",
       "      <th>VCF_REF</th>\n",
       "      <th>VCF_ALT</th>\n",
       "      <th>VCF_QUAL_2</th>\n",
       "      <th>VCF_FILTER</th>\n",
       "      <th>VCF_INFO</th>\n",
       "      <th>VCF_FORMAT</th>\n",
       "      <th>VCF_SAMPLE_NAME</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>82</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41196822</td>\n",
       "      <td>41196824</td>\n",
       "      <td>TTT</td>\n",
       "      <td>-</td>\n",
       "      <td>UTR3</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>NM_007300:c.*873_*871delAAA;NM_007297:c.*873_*...</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41196821</td>\n",
       "      <td>.</td>\n",
       "      <td>CTTT</td>\n",
       "      <td>C</td>\n",
       "      <td>84.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.689;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:109,19:128:99:1:0.5:122,0,3145:69,40,12,7:...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>83</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41197940</td>\n",
       "      <td>41197940</td>\n",
       "      <td>T</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41197939</td>\n",
       "      <td>.</td>\n",
       "      <td>AT</td>\n",
       "      <td>A</td>\n",
       "      <td>1932.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=3.135;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:60,107:167:99:1:0.5:1970,0,654:2,58,33,74:...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>84</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41247621</td>\n",
       "      <td>41247621</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41247620</td>\n",
       "      <td>.</td>\n",
       "      <td>CA</td>\n",
       "      <td>C</td>\n",
       "      <td>197.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.097;DP=38;Exc...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:24,13:38:99:235,0,434</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>85</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41256075</td>\n",
       "      <td>41256075</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41256074</td>\n",
       "      <td>.</td>\n",
       "      <td>CA</td>\n",
       "      <td>C</td>\n",
       "      <td>932.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=0.364;DP=201;Exc...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:128,67:201:99:970,0,2530</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>86</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41256088</td>\n",
       "      <td>41256088</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41256087</td>\n",
       "      <td>.</td>\n",
       "      <td>GA</td>\n",
       "      <td>G</td>\n",
       "      <td>357.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-0.38;ClippingRa...</td>\n",
       "      <td>GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB</td>\n",
       "      <td>0/1:83,28:111:99:1:0.5:395,0,2036:83,0,28,0:70...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>87</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41258136</td>\n",
       "      <td>41258136</td>\n",
       "      <td>A</td>\n",
       "      <td>-</td>\n",
       "      <td>intronic</td>\n",
       "      <td>BRCA1</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>.</td>\n",
       "      <td>...</td>\n",
       "      <td>chr17</td>\n",
       "      <td>41258135</td>\n",
       "      <td>.</td>\n",
       "      <td>TA</td>\n",
       "      <td>T</td>\n",
       "      <td>203.73</td>\n",
       "      <td>.</td>\n",
       "      <td>AC=1;AF=0.5;AN=2;BaseQRankSum=-1.019;DP=69;Exc...</td>\n",
       "      <td>GT:AD:DP:GQ:PL</td>\n",
       "      <td>0/1:36,24:69:99:241,0,424</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>6 rows × 183 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr     Start       End  Ref Alt Func.refGene Gene.refGene  \\\n",
       "82  chr17  41196822  41196824  TTT   -         UTR3        BRCA1   \n",
       "83  chr17  41197940  41197940    T   -     intronic        BRCA1   \n",
       "84  chr17  41247621  41247621    A   -     intronic        BRCA1   \n",
       "85  chr17  41256075  41256075    A   -     intronic        BRCA1   \n",
       "86  chr17  41256088  41256088    A   -     intronic        BRCA1   \n",
       "87  chr17  41258136  41258136    A   -     intronic        BRCA1   \n",
       "\n",
       "                                   GeneDetail.refGene ExonicFunc.refGene  \\\n",
       "82  NM_007300:c.*873_*871delAAA;NM_007297:c.*873_*...                  .   \n",
       "83                                                  .                  .   \n",
       "84                                                  .                  .   \n",
       "85                                                  .                  .   \n",
       "86                                                  .                  .   \n",
       "87                                                  .                  .   \n",
       "\n",
       "   AAChange.refGene  ... VCF_CHR   VCF_POS VCF_ID VCF_REF VCF_ALT VCF_QUAL_2  \\\n",
       "82                .  ...   chr17  41196821      .    CTTT       C      84.73   \n",
       "83                .  ...   chr17  41197939      .      AT       A    1932.73   \n",
       "84                .  ...   chr17  41247620      .      CA       C     197.73   \n",
       "85                .  ...   chr17  41256074      .      CA       C     932.73   \n",
       "86                .  ...   chr17  41256087      .      GA       G     357.73   \n",
       "87                .  ...   chr17  41258135      .      TA       T     203.73   \n",
       "\n",
       "   VCF_FILTER                                           VCF_INFO  \\\n",
       "82          .  AC=1;AF=0.5;AN=2;BaseQRankSum=0.689;ClippingRa...   \n",
       "83          .  AC=1;AF=0.5;AN=2;BaseQRankSum=3.135;ClippingRa...   \n",
       "84          .  AC=1;AF=0.5;AN=2;BaseQRankSum=-0.097;DP=38;Exc...   \n",
       "85          .  AC=1;AF=0.5;AN=2;BaseQRankSum=0.364;DP=201;Exc...   \n",
       "86          .  AC=1;AF=0.5;AN=2;BaseQRankSum=-0.38;ClippingRa...   \n",
       "87          .  AC=1;AF=0.5;AN=2;BaseQRankSum=-1.019;DP=69;Exc...   \n",
       "\n",
       "                             VCF_FORMAT  \\\n",
       "82  GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "83  GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "84                       GT:AD:DP:GQ:PL   \n",
       "85                       GT:AD:DP:GQ:PL   \n",
       "86  GT:AD:DP:GQ:MLPSAC:MLPSAF:PL:SAC:SB   \n",
       "87                       GT:AD:DP:GQ:PL   \n",
       "\n",
       "                                      VCF_SAMPLE_NAME  \n",
       "82  0/1:109,19:128:99:1:0.5:122,0,3145:69,40,12,7:...  \n",
       "83  0/1:60,107:167:99:1:0.5:1970,0,654:2,58,33,74:...  \n",
       "84                          0/1:24,13:38:99:235,0,434  \n",
       "85                       0/1:128,67:201:99:970,0,2530  \n",
       "86  0/1:83,28:111:99:1:0.5:395,0,2036:83,0,28,0:70...  \n",
       "87                          0/1:36,24:69:99:241,0,424  \n",
       "\n",
       "[6 rows x 183 columns]"
      ]
     },
     "execution_count": 65,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "multianno_df.query(\"`Gene.refGene` == 'BRCA1'\")"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### Trabalhando com dados faltantes"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### Agrupando um `DataFrame`"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "Assim como no `SQL` o pandas nos permite computar agregações em nossos `DataFrame`s. Para atingir tal objetivo, usaremos a função [`groupby`](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.DataFrame.groupby.html). O mecanimos por trás dessa função foge do escopo desse curso, contudo é importante notar que a metodologia usada para atingir essa funcionalidade é conhecida como *split-apply-combine*."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 84,
   "metadata": {},
   "outputs": [
    {
     "data": {
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       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
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       "      <td>chr10</td>\n",
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       "      <td>NaN</td>\n",
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       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142.0</td>\n",
       "      <td>89623142.0</td>\n",
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       "      <th>2</th>\n",
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       "      <td>89623861.0</td>\n",
       "      <td>89623861.0</td>\n",
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       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
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       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
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      ],
      "text/plain": [
       "     Chr       Start         End\n",
       "0  chr10  89622938.0         NaN\n",
       "1  chr10  89623142.0  89623142.0\n",
       "2    NaN  89623861.0  89623861.0\n",
       "3  chr10  89623901.0  89623901.0\n",
       "4  chr10         NaN         NaN"
      ]
     },
     "execution_count": 84,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "uri = \"https://www.dropbox.com/s/lsux24ny8tr123i/multianno_filtrado.csv?dl=1\"\n",
    "bed = pd.read_csv(uri)\n",
    "bed.head()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Para substituir dados faltantes por um outro valor, basta usar a função `fillna()`"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 85,
   "metadata": {},
   "outputs": [
    {
     "data": {
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       "      <th></th>\n",
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       "      <td>89623142.0</td>\n",
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       "      <th>4</th>\n",
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       "      <th>87</th>\n",
       "      <td>chr17</td>\n",
       "      <td>0.0</td>\n",
       "      <td>41258136.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>88</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1206797.0</td>\n",
       "      <td>1206797.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>89</th>\n",
       "      <td>0</td>\n",
       "      <td>1219444.0</td>\n",
       "      <td>1219444.0</td>\n",
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       "    <tr>\n",
       "      <th>90</th>\n",
       "      <td>0</td>\n",
       "      <td>0.0</td>\n",
       "      <td>1219451.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>91</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418.0</td>\n",
       "      <td>1221418.0</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>92 rows × 3 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr       Start         End\n",
       "0   chr10  89622938.0         0.0\n",
       "1   chr10  89623142.0  89623142.0\n",
       "2       0  89623861.0  89623861.0\n",
       "3   chr10  89623901.0  89623901.0\n",
       "4   chr10         0.0         0.0\n",
       "..    ...         ...         ...\n",
       "87  chr17         0.0  41258136.0\n",
       "88  chr19   1206797.0   1206797.0\n",
       "89      0   1219444.0   1219444.0\n",
       "90      0         0.0   1219451.0\n",
       "91  chr19   1221418.0   1221418.0\n",
       "\n",
       "[92 rows x 3 columns]"
      ]
     },
     "execution_count": 85,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "bed.fillna(0)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Para espeficiar novos valores para os dados faltantes, pode se específicar quais dados para quais colunas usand dicionários."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 86,
   "metadata": {},
   "outputs": [
    {
     "data": {
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       "      <th>87</th>\n",
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       "      <th>88</th>\n",
       "      <td>chr19</td>\n",
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       "      <td>1206797.0</td>\n",
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       "    <tr>\n",
       "      <th>89</th>\n",
       "      <td>chr0</td>\n",
       "      <td>1219444.0</td>\n",
       "      <td>1219444.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>90</th>\n",
       "      <td>chr0</td>\n",
       "      <td>0.0</td>\n",
       "      <td>1219451.0</td>\n",
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       "    <tr>\n",
       "      <th>91</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418.0</td>\n",
       "      <td>1221418.0</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>92 rows × 3 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr       Start         End\n",
       "0   chr10  89622938.0         0.0\n",
       "1   chr10  89623142.0  89623142.0\n",
       "2    chr0  89623861.0  89623861.0\n",
       "3   chr10  89623901.0  89623901.0\n",
       "4   chr10         0.0         0.0\n",
       "..    ...         ...         ...\n",
       "87  chr17         0.0  41258136.0\n",
       "88  chr19   1206797.0   1206797.0\n",
       "89   chr0   1219444.0   1219444.0\n",
       "90   chr0         0.0   1219451.0\n",
       "91  chr19   1221418.0   1221418.0\n",
       "\n",
       "[92 rows x 3 columns]"
      ]
     },
     "execution_count": 86,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "bed.fillna({\n",
    "    \"Chr\": \"chr0\",\n",
    "    \"Start\": 0,\n",
    "    \"End\": 0\n",
    "})"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    " * Existem alguns métodos no `fillna()` que facilitam a troca do dado faltante."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 87,
   "metadata": {},
   "outputs": [
    {
     "data": {
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       "      <th>88</th>\n",
       "      <td>chr19</td>\n",
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       "      <td>1206797.0</td>\n",
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       "    <tr>\n",
       "      <th>89</th>\n",
       "      <td>chr19</td>\n",
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       "      <td>1219444.0</td>\n",
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       "    <tr>\n",
       "      <th>90</th>\n",
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       "      <td>1219444.0</td>\n",
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       "      <th>91</th>\n",
       "      <td>chr19</td>\n",
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       "      <td>1221418.0</td>\n",
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       "</table>\n",
       "<p>92 rows × 3 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr       Start         End\n",
       "0   chr10  89622938.0         NaN\n",
       "1   chr10  89623142.0  89623142.0\n",
       "2   chr10  89623861.0  89623861.0\n",
       "3   chr10  89623901.0  89623901.0\n",
       "4   chr10  89623901.0  89623901.0\n",
       "..    ...         ...         ...\n",
       "87  chr17  41256088.0  41258136.0\n",
       "88  chr19   1206797.0   1206797.0\n",
       "89  chr19   1219444.0   1219444.0\n",
       "90  chr19   1219444.0   1219451.0\n",
       "91  chr19   1221418.0   1221418.0\n",
       "\n",
       "[92 rows x 3 columns]"
      ]
     },
     "execution_count": 87,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# ffill pega o valor anterior e substitui o dado faltante\n",
    "bed.fillna(method=\"ffill\")"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 88,
   "metadata": {},
   "outputs": [
    {
     "data": {
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       "      <th>88</th>\n",
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       "      <td>1206797.0</td>\n",
       "      <td>1206797.0</td>\n",
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       "    <tr>\n",
       "      <th>89</th>\n",
       "      <td>chr19</td>\n",
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       "      <th>90</th>\n",
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       "      <td>1221418.0</td>\n",
       "      <td>1219451.0</td>\n",
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       "    <tr>\n",
       "      <th>91</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418.0</td>\n",
       "      <td>1221418.0</td>\n",
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       "  </tbody>\n",
       "</table>\n",
       "<p>92 rows × 3 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr       Start         End\n",
       "0   chr10  89622938.0  89623142.0\n",
       "1   chr10  89623142.0  89623142.0\n",
       "2   chr10  89623861.0  89623861.0\n",
       "3   chr10  89623901.0  89623901.0\n",
       "4   chr10  89654190.0  89654190.0\n",
       "..    ...         ...         ...\n",
       "87  chr17   1206797.0  41258136.0\n",
       "88  chr19   1206797.0   1206797.0\n",
       "89  chr19   1219444.0   1219444.0\n",
       "90  chr19   1221418.0   1219451.0\n",
       "91  chr19   1221418.0   1221418.0\n",
       "\n",
       "[92 rows x 3 columns]"
      ]
     },
     "execution_count": 88,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# bfill pega o valor posterior e substitui o dado faltante\n",
    "bed.fillna(method=\"bfill\")"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 89,
   "metadata": {},
   "outputs": [
    {
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       "    </tr>\n",
       "    <tr>\n",
       "      <th>89</th>\n",
       "      <td>1.21944e+06</td>\n",
       "      <td>1.21944e+06</td>\n",
       "      <td>1.21944e+06</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>90</th>\n",
       "      <td>1.21945e+06</td>\n",
       "      <td>1.21945e+06</td>\n",
       "      <td>1.21945e+06</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>91</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1.22142e+06</td>\n",
       "      <td>1.22142e+06</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>92 rows × 3 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "            Chr        Start          End\n",
       "0         chr10  8.96229e+07          NaN\n",
       "1         chr10  8.96231e+07  8.96231e+07\n",
       "2   8.96239e+07  8.96239e+07  8.96239e+07\n",
       "3         chr10  8.96239e+07  8.96239e+07\n",
       "4         chr10          NaN          NaN\n",
       "..          ...          ...          ...\n",
       "87        chr17  4.12581e+07  4.12581e+07\n",
       "88        chr19   1.2068e+06   1.2068e+06\n",
       "89  1.21944e+06  1.21944e+06  1.21944e+06\n",
       "90  1.21945e+06  1.21945e+06  1.21945e+06\n",
       "91        chr19  1.22142e+06  1.22142e+06\n",
       "\n",
       "[92 rows x 3 columns]"
      ]
     },
     "execution_count": 89,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "bed.fillna(method=\"bfill\", axis=\"columns\") # horizontal"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 90,
   "metadata": {},
   "outputs": [
    {
     "data": {
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       "<div>\n",
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       "    .dataframe tbody tr th:only-of-type {\n",
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       "\n",
       "    .dataframe tbody tr th {\n",
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       "\n",
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       "</style>\n",
       "<table border=\"1\" class=\"dataframe\">\n",
       "  <thead>\n",
       "    <tr style=\"text-align: right;\">\n",
       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
       "    </tr>\n",
       "  </thead>\n",
       "  <tbody>\n",
       "    <tr>\n",
       "      <th>0</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89622938.0</td>\n",
       "      <td>89623142.0</td>\n",
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       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142.0</td>\n",
       "      <td>89623142.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623861.0</td>\n",
       "      <td>89623861.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901.0</td>\n",
       "      <td>89623901.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190.0</td>\n",
       "      <td>89654190.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>...</th>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "      <td>...</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>87</th>\n",
       "      <td>chr17</td>\n",
       "      <td>1206797.0</td>\n",
       "      <td>41258136.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>88</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1206797.0</td>\n",
       "      <td>1206797.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>89</th>\n",
       "      <td>NaN</td>\n",
       "      <td>1219444.0</td>\n",
       "      <td>1219444.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>90</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418.0</td>\n",
       "      <td>1219451.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>91</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418.0</td>\n",
       "      <td>1221418.0</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>92 rows × 3 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr       Start         End\n",
       "0   chr10  89622938.0  89623142.0\n",
       "1   chr10  89623142.0  89623142.0\n",
       "2   chr10  89623861.0  89623861.0\n",
       "3   chr10  89623901.0  89623901.0\n",
       "4   chr10  89654190.0  89654190.0\n",
       "..    ...         ...         ...\n",
       "87  chr17   1206797.0  41258136.0\n",
       "88  chr19   1206797.0   1206797.0\n",
       "89    NaN   1219444.0   1219444.0\n",
       "90  chr19   1221418.0   1219451.0\n",
       "91  chr19   1221418.0   1221418.0\n",
       "\n",
       "[92 rows x 3 columns]"
      ]
     },
     "execution_count": 90,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "bed.fillna(method=\"bfill\", limit=1) # apenas uma copia"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Na documentação do [`fillna()`](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.DataFrame.fillna.html) existem outros métodos que podem ser utilizados.\n",
    "* Para deletar as linhas que contém algum dado faltante use a função `dropna()`"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 91,
   "metadata": {
    "scrolled": true
   },
   "outputs": [
    {
     "data": {
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       "  <thead>\n",
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       "      <th></th>\n",
       "      <th>Chr</th>\n",
       "      <th>Start</th>\n",
       "      <th>End</th>\n",
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       "  <tbody>\n",
       "    <tr>\n",
       "      <th>1</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142.0</td>\n",
       "      <td>89623142.0</td>\n",
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       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901.0</td>\n",
       "      <td>89623901.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>5</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190.0</td>\n",
       "      <td>89654190.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>7</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89718057.0</td>\n",
       "      <td>89718057.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>10</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720907.0</td>\n",
       "      <td>89720907.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>12</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725294.0</td>\n",
       "      <td>89725294.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>13</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582.0</td>\n",
       "      <td>89725582.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>14</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32889968.0</td>\n",
       "      <td>32889968.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>17</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32905220.0</td>\n",
       "      <td>32905220.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>19</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32907536.0</td>\n",
       "      <td>32907536.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>20</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32911888.0</td>\n",
       "      <td>32911888.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>23</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32915005.0</td>\n",
       "      <td>32915005.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>24</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32915411.0</td>\n",
       "      <td>32915414.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>26</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32919637.0</td>\n",
       "      <td>32919637.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>27</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32920016.0</td>\n",
       "      <td>32920016.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>28</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32920618.0</td>\n",
       "      <td>32920618.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>29</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32920844.0</td>\n",
       "      <td>32920844.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>30</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32929058.0</td>\n",
       "      <td>32929059.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>31</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32929232.0</td>\n",
       "      <td>32929232.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>32</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32929387.0</td>\n",
       "      <td>32929387.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>33</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32930373.0</td>\n",
       "      <td>32930373.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>34</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32936646.0</td>\n",
       "      <td>32936646.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>37</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32951115.0</td>\n",
       "      <td>32951115.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>39</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32954561.0</td>\n",
       "      <td>32954561.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>40</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32954692.0</td>\n",
       "      <td>32954692.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>41</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32954741.0</td>\n",
       "      <td>32954741.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>43</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32968607.0</td>\n",
       "      <td>32968607.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>44</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32970736.0</td>\n",
       "      <td>32970736.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>45</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32971425.0</td>\n",
       "      <td>32971425.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>46</th>\n",
       "      <td>chr13</td>\n",
       "      <td>32973012.0</td>\n",
       "      <td>32973012.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>47</th>\n",
       "      <td>chr16</td>\n",
       "      <td>23615043.0</td>\n",
       "      <td>23615043.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>48</th>\n",
       "      <td>chr16</td>\n",
       "      <td>23640160.0</td>\n",
       "      <td>23640160.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>49</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68770847.0</td>\n",
       "      <td>68770847.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>51</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68771372.0</td>\n",
       "      <td>68771372.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>53</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68771547.0</td>\n",
       "      <td>68771547.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>54</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68772681.0</td>\n",
       "      <td>68772681.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>55</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68820717.0</td>\n",
       "      <td>68820717.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>57</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68847692.0</td>\n",
       "      <td>68847692.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>58</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68847694.0</td>\n",
       "      <td>68847694.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>60</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68847719.0</td>\n",
       "      <td>68847719.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>61</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68847726.0</td>\n",
       "      <td>68847726.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>62</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68847727.0</td>\n",
       "      <td>68847727.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>63</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68855852.0</td>\n",
       "      <td>68855852.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>64</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68856474.0</td>\n",
       "      <td>68856474.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>66</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68857792.0</td>\n",
       "      <td>68857792.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>67</th>\n",
       "      <td>chr16</td>\n",
       "      <td>68867612.0</td>\n",
       "      <td>68867612.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>68</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7576265.0</td>\n",
       "      <td>7576265.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>70</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7577679.0</td>\n",
       "      <td>7577679.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>71</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7578115.0</td>\n",
       "      <td>7578115.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>72</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7578645.0</td>\n",
       "      <td>7578645.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>74</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7578712.0</td>\n",
       "      <td>7578714.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>75</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7578837.0</td>\n",
       "      <td>7578837.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>76</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7579472.0</td>\n",
       "      <td>7579472.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>78</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7579801.0</td>\n",
       "      <td>7579801.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>80</th>\n",
       "      <td>chr17</td>\n",
       "      <td>7590601.0</td>\n",
       "      <td>7590601.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>82</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41196822.0</td>\n",
       "      <td>41196824.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>83</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41197940.0</td>\n",
       "      <td>41197940.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>84</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41247621.0</td>\n",
       "      <td>41247621.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>88</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1206797.0</td>\n",
       "      <td>1206797.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>91</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418.0</td>\n",
       "      <td>1221418.0</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr       Start         End\n",
       "1   chr10  89623142.0  89623142.0\n",
       "3   chr10  89623901.0  89623901.0\n",
       "5   chr10  89654190.0  89654190.0\n",
       "7   chr10  89718057.0  89718057.0\n",
       "10  chr10  89720907.0  89720907.0\n",
       "12  chr10  89725294.0  89725294.0\n",
       "13  chr10  89725582.0  89725582.0\n",
       "14  chr13  32889968.0  32889968.0\n",
       "17  chr13  32905220.0  32905220.0\n",
       "19  chr13  32907536.0  32907536.0\n",
       "20  chr13  32911888.0  32911888.0\n",
       "23  chr13  32915005.0  32915005.0\n",
       "24  chr13  32915411.0  32915414.0\n",
       "26  chr13  32919637.0  32919637.0\n",
       "27  chr13  32920016.0  32920016.0\n",
       "28  chr13  32920618.0  32920618.0\n",
       "29  chr13  32920844.0  32920844.0\n",
       "30  chr13  32929058.0  32929059.0\n",
       "31  chr13  32929232.0  32929232.0\n",
       "32  chr13  32929387.0  32929387.0\n",
       "33  chr13  32930373.0  32930373.0\n",
       "34  chr13  32936646.0  32936646.0\n",
       "37  chr13  32951115.0  32951115.0\n",
       "39  chr13  32954561.0  32954561.0\n",
       "40  chr13  32954692.0  32954692.0\n",
       "41  chr13  32954741.0  32954741.0\n",
       "43  chr13  32968607.0  32968607.0\n",
       "44  chr13  32970736.0  32970736.0\n",
       "45  chr13  32971425.0  32971425.0\n",
       "46  chr13  32973012.0  32973012.0\n",
       "47  chr16  23615043.0  23615043.0\n",
       "48  chr16  23640160.0  23640160.0\n",
       "49  chr16  68770847.0  68770847.0\n",
       "51  chr16  68771372.0  68771372.0\n",
       "53  chr16  68771547.0  68771547.0\n",
       "54  chr16  68772681.0  68772681.0\n",
       "55  chr16  68820717.0  68820717.0\n",
       "57  chr16  68847692.0  68847692.0\n",
       "58  chr16  68847694.0  68847694.0\n",
       "60  chr16  68847719.0  68847719.0\n",
       "61  chr16  68847726.0  68847726.0\n",
       "62  chr16  68847727.0  68847727.0\n",
       "63  chr16  68855852.0  68855852.0\n",
       "64  chr16  68856474.0  68856474.0\n",
       "66  chr16  68857792.0  68857792.0\n",
       "67  chr16  68867612.0  68867612.0\n",
       "68  chr17   7576265.0   7576265.0\n",
       "70  chr17   7577679.0   7577679.0\n",
       "71  chr17   7578115.0   7578115.0\n",
       "72  chr17   7578645.0   7578645.0\n",
       "74  chr17   7578712.0   7578714.0\n",
       "75  chr17   7578837.0   7578837.0\n",
       "76  chr17   7579472.0   7579472.0\n",
       "78  chr17   7579801.0   7579801.0\n",
       "80  chr17   7590601.0   7590601.0\n",
       "82  chr17  41196822.0  41196824.0\n",
       "83  chr17  41197940.0  41197940.0\n",
       "84  chr17  41247621.0  41247621.0\n",
       "88  chr19   1206797.0   1206797.0\n",
       "91  chr19   1221418.0   1221418.0"
      ]
     },
     "execution_count": 91,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "bed.dropna()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Para deletar apenas linhas em que todos os valores são faltantes use o argumento `how=\"all\"` dentro da função `dropna()`;\n",
    "* Para especificar o valor limite de valores que devem ter em uma linha você pode usar o argumento `thresh`."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 92,
   "metadata": {},
   "outputs": [
    {
     "data": {
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       "      <td>89623901.0</td>\n",
       "      <td>89623901.0</td>\n",
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       "      <th>...</th>\n",
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       "    <tr>\n",
       "      <th>86</th>\n",
       "      <td>chr17</td>\n",
       "      <td>41256088.0</td>\n",
       "      <td>NaN</td>\n",
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       "    <tr>\n",
       "      <th>87</th>\n",
       "      <td>chr17</td>\n",
       "      <td>NaN</td>\n",
       "      <td>41258136.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>88</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1206797.0</td>\n",
       "      <td>1206797.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>89</th>\n",
       "      <td>NaN</td>\n",
       "      <td>1219444.0</td>\n",
       "      <td>1219444.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>91</th>\n",
       "      <td>chr19</td>\n",
       "      <td>1221418.0</td>\n",
       "      <td>1221418.0</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "<p>90 rows × 3 columns</p>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr       Start         End\n",
       "0   chr10  89622938.0         NaN\n",
       "1   chr10  89623142.0  89623142.0\n",
       "2     NaN  89623861.0  89623861.0\n",
       "3   chr10  89623901.0  89623901.0\n",
       "5   chr10  89654190.0  89654190.0\n",
       "..    ...         ...         ...\n",
       "86  chr17  41256088.0         NaN\n",
       "87  chr17         NaN  41258136.0\n",
       "88  chr19   1206797.0   1206797.0\n",
       "89    NaN   1219444.0   1219444.0\n",
       "91  chr19   1221418.0   1221418.0\n",
       "\n",
       "[90 rows x 3 columns]"
      ]
     },
     "execution_count": 92,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "bed.dropna(thresh=2)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Caso arquivo venha com dados faltantes mas não como `NaN` mas como algum valor padrão de quem enviou os dados, como transformar para `NaN`?"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 95,
   "metadata": {},
   "outputs": [
    {
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       "    <tr>\n",
       "      <th>13</th>\n",
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       "      <td>89725582</td>\n",
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       "</table>\n",
       "</div>"
      ],
      "text/plain": [
       "       Chr     Start       End\n",
       "0    chr10  89622938    -88888\n",
       "1    chr10  89623142  89623142\n",
       "2   -99999  89623861  89623861\n",
       "3    chr10  89623901  89623901\n",
       "4    chr10         0    -88888\n",
       "5    chr10  89654190  89654190\n",
       "6    chr10         0  89711576\n",
       "7    chr10  89718057  89718057\n",
       "8    chr10  89720548    -88888\n",
       "9    chr10         0  89720634\n",
       "10   chr10  89720907  89720907\n",
       "11  -99999  89721094  89721094\n",
       "12   chr10  89725294  89725294\n",
       "13   chr10  89725582  89725582"
      ]
     },
     "execution_count": 95,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "uri = \"https://www.dropbox.com/s/0chortbsbpjcdjb/multianno_filtrado_replace.csv?dl=1\"\n",
    "lit_bed = pd.read_csv(uri)\n",
    "lit_bed"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 99,
   "metadata": {},
   "outputs": [
    {
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       "      <th>6</th>\n",
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       "    <tr>\n",
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       "      <td>89718057</td>\n",
       "      <td>89718057</td>\n",
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       "      <td>-88888</td>\n",
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       "    <tr>\n",
       "      <th>9</th>\n",
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       "      <td>0</td>\n",
       "      <td>89720634</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>10</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720907</td>\n",
       "      <td>89720907</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>11</th>\n",
       "      <td>NaN</td>\n",
       "      <td>89721094</td>\n",
       "      <td>89721094</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>12</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725294</td>\n",
       "      <td>89725294</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>13</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582</td>\n",
       "      <td>89725582</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr     Start       End\n",
       "0   chr10  89622938    -88888\n",
       "1   chr10  89623142  89623142\n",
       "2     NaN  89623861  89623861\n",
       "3   chr10  89623901  89623901\n",
       "4   chr10         0    -88888\n",
       "5   chr10  89654190  89654190\n",
       "6   chr10         0  89711576\n",
       "7   chr10  89718057  89718057\n",
       "8   chr10  89720548    -88888\n",
       "9   chr10         0  89720634\n",
       "10  chr10  89720907  89720907\n",
       "11    NaN  89721094  89721094\n",
       "12  chr10  89725294  89725294\n",
       "13  chr10  89725582  89725582"
      ]
     },
     "execution_count": 99,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "import numpy as np\n",
    "lit_bed.replace(\"-99999\", np.NaN)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 100,
   "metadata": {},
   "outputs": [
    {
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       "      <th>6</th>\n",
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       "      <td>NaN</td>\n",
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       "    <tr>\n",
       "      <th>9</th>\n",
       "      <td>chr10</td>\n",
       "      <td>0</td>\n",
       "      <td>89720634.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>10</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720907</td>\n",
       "      <td>89720907.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>11</th>\n",
       "      <td>NaN</td>\n",
       "      <td>89721094</td>\n",
       "      <td>89721094.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>12</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725294</td>\n",
       "      <td>89725294.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>13</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582</td>\n",
       "      <td>89725582.0</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr     Start         End\n",
       "0   chr10  89622938         NaN\n",
       "1   chr10  89623142  89623142.0\n",
       "2     NaN  89623861  89623861.0\n",
       "3   chr10  89623901  89623901.0\n",
       "4   chr10         0         NaN\n",
       "5   chr10  89654190  89654190.0\n",
       "6   chr10         0  89711576.0\n",
       "7   chr10  89718057  89718057.0\n",
       "8   chr10  89720548         NaN\n",
       "9   chr10         0  89720634.0\n",
       "10  chr10  89720907  89720907.0\n",
       "11    NaN  89721094  89721094.0\n",
       "12  chr10  89725294  89725294.0\n",
       "13  chr10  89725582  89725582.0"
      ]
     },
     "execution_count": 100,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "lit_bed.replace([\"-99999\", -88888], np.NaN)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 101,
   "metadata": {},
   "outputs": [
    {
     "data": {
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       "      <th>1</th>\n",
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       "      <td>89623142.0</td>\n",
       "      <td>89623142.0</td>\n",
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       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>NaN</td>\n",
       "      <td>89623861.0</td>\n",
       "      <td>89623861.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901.0</td>\n",
       "      <td>89623901.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
       "      <td>NaN</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>5</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190.0</td>\n",
       "      <td>89654190.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>6</th>\n",
       "      <td>chr10</td>\n",
       "      <td>NaN</td>\n",
       "      <td>89711576.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>7</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89718057.0</td>\n",
       "      <td>89718057.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>8</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720548.0</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>9</th>\n",
       "      <td>chr10</td>\n",
       "      <td>NaN</td>\n",
       "      <td>89720634.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>10</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720907.0</td>\n",
       "      <td>89720907.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>11</th>\n",
       "      <td>NaN</td>\n",
       "      <td>89721094.0</td>\n",
       "      <td>89721094.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>12</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725294.0</td>\n",
       "      <td>89725294.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>13</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582.0</td>\n",
       "      <td>89725582.0</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr       Start         End\n",
       "0   chr10  89622938.0         NaN\n",
       "1   chr10  89623142.0  89623142.0\n",
       "2     NaN  89623861.0  89623861.0\n",
       "3   chr10  89623901.0  89623901.0\n",
       "4   chr10         NaN         NaN\n",
       "5   chr10  89654190.0  89654190.0\n",
       "6   chr10         NaN  89711576.0\n",
       "7   chr10  89718057.0  89718057.0\n",
       "8   chr10  89720548.0         NaN\n",
       "9   chr10         NaN  89720634.0\n",
       "10  chr10  89720907.0  89720907.0\n",
       "11    NaN  89721094.0  89721094.0\n",
       "12  chr10  89725294.0  89725294.0\n",
       "13  chr10  89725582.0  89725582.0"
      ]
     },
     "execution_count": 101,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "lit_bed.replace([\"-99999\", -88888, 0], np.NaN)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 105,
   "metadata": {},
   "outputs": [
    {
     "data": {
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       "  <tbody>\n",
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       "      <th>0</th>\n",
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       "      <th>1</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623142.0</td>\n",
       "      <td>89623142.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>2</th>\n",
       "      <td>chr0</td>\n",
       "      <td>89623861.0</td>\n",
       "      <td>89623861.0</td>\n",
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       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901.0</td>\n",
       "      <td>89623901.0</td>\n",
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       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
       "      <td>0.0</td>\n",
       "      <td>NaN</td>\n",
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       "    <tr>\n",
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       "      <td>chr10</td>\n",
       "      <td>89654190.0</td>\n",
       "      <td>89654190.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>6</th>\n",
       "      <td>chr10</td>\n",
       "      <td>0.0</td>\n",
       "      <td>89711576.0</td>\n",
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       "    <tr>\n",
       "      <th>7</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89718057.0</td>\n",
       "      <td>89718057.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>8</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720548.0</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>9</th>\n",
       "      <td>chr10</td>\n",
       "      <td>0.0</td>\n",
       "      <td>89720634.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>10</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720907.0</td>\n",
       "      <td>89720907.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>11</th>\n",
       "      <td>chr0</td>\n",
       "      <td>89721094.0</td>\n",
       "      <td>89721094.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>12</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725294.0</td>\n",
       "      <td>89725294.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>13</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582.0</td>\n",
       "      <td>89725582.0</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr       Start         End\n",
       "0   chr10  89622938.0         NaN\n",
       "1   chr10  89623142.0  89623142.0\n",
       "2    chr0  89623861.0  89623861.0\n",
       "3   chr10  89623901.0  89623901.0\n",
       "4   chr10         0.0         NaN\n",
       "5   chr10  89654190.0  89654190.0\n",
       "6   chr10         0.0  89711576.0\n",
       "7   chr10  89718057.0  89718057.0\n",
       "8   chr10  89720548.0         NaN\n",
       "9   chr10         0.0  89720634.0\n",
       "10  chr10  89720907.0  89720907.0\n",
       "11   chr0  89721094.0  89721094.0\n",
       "12  chr10  89725294.0  89725294.0\n",
       "13  chr10  89725582.0  89725582.0"
      ]
     },
     "execution_count": 105,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# Especifique o novo valor usando dicionarios\n",
    "lit_bed.replace({\n",
    "    \"-99999\": \"chr0\",\n",
    "    -88888: np.NaN\n",
    "})"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Caso exista `0` em algumas das colunas e você não queira substituir, você pode especificar os valores a serem substituídos por coluna a partir de um dicionário"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 104,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": [
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       "      <th>0</th>\n",
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       "      <th>2</th>\n",
       "      <td>NaN</td>\n",
       "      <td>89623861.0</td>\n",
       "      <td>89623861.0</td>\n",
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       "    <tr>\n",
       "      <th>3</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89623901.0</td>\n",
       "      <td>89623901.0</td>\n",
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       "    <tr>\n",
       "      <th>4</th>\n",
       "      <td>chr10</td>\n",
       "      <td>NaN</td>\n",
       "      <td>NaN</td>\n",
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       "    <tr>\n",
       "      <th>5</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89654190.0</td>\n",
       "      <td>89654190.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>6</th>\n",
       "      <td>chr10</td>\n",
       "      <td>NaN</td>\n",
       "      <td>89711576.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>7</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89718057.0</td>\n",
       "      <td>89718057.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>8</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720548.0</td>\n",
       "      <td>NaN</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>9</th>\n",
       "      <td>chr10</td>\n",
       "      <td>NaN</td>\n",
       "      <td>89720634.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>10</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89720907.0</td>\n",
       "      <td>89720907.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>11</th>\n",
       "      <td>NaN</td>\n",
       "      <td>89721094.0</td>\n",
       "      <td>89721094.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>12</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725294.0</td>\n",
       "      <td>89725294.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>13</th>\n",
       "      <td>chr10</td>\n",
       "      <td>89725582.0</td>\n",
       "      <td>89725582.0</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "</div>"
      ],
      "text/plain": [
       "      Chr       Start         End\n",
       "0   chr10  89622938.0         NaN\n",
       "1   chr10  89623142.0  89623142.0\n",
       "2     NaN  89623861.0  89623861.0\n",
       "3   chr10  89623901.0  89623901.0\n",
       "4   chr10         NaN         NaN\n",
       "5   chr10  89654190.0  89654190.0\n",
       "6   chr10         NaN  89711576.0\n",
       "7   chr10  89718057.0  89718057.0\n",
       "8   chr10  89720548.0         NaN\n",
       "9   chr10         NaN  89720634.0\n",
       "10  chr10  89720907.0  89720907.0\n",
       "11    NaN  89721094.0  89721094.0\n",
       "12  chr10  89725294.0  89725294.0\n",
       "13  chr10  89725582.0  89725582.0"
      ]
     },
     "execution_count": 104,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "lit_bed.replace({\n",
    "    \"Chr\": \"-99999\",\n",
    "    \"Start\": 0,\n",
    "    \"End\": -88888\n",
    "}, np.NaN)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "* Um exemplo de substituição de valores"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 110,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "array(['chr10', 'chr13', 'chr16', 'chr17', 'chr19'], dtype=object)"
      ]
     },
     "execution_count": 110,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "ex = bed.dropna()\n",
    "ex.Chr.unique()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 111,
   "metadata": {
    "scrolled": true
   },
   "outputs": [
    {
     "data": {
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       "      <th>5</th>\n",
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       "      <td>89654190.0</td>\n",
       "      <td>89654190.0</td>\n",
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       "    <tr>\n",
       "      <th>7</th>\n",
       "      <td>10</td>\n",
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       "      <th>10</th>\n",
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       "    <tr>\n",
       "      <th>12</th>\n",
       "      <td>10</td>\n",
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       "      <td>89725294.0</td>\n",
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       "    <tr>\n",
       "      <th>13</th>\n",
       "      <td>10</td>\n",
       "      <td>89725582.0</td>\n",
       "      <td>89725582.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>14</th>\n",
       "      <td>13</td>\n",
       "      <td>32889968.0</td>\n",
       "      <td>32889968.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>17</th>\n",
       "      <td>13</td>\n",
       "      <td>32905220.0</td>\n",
       "      <td>32905220.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>19</th>\n",
       "      <td>13</td>\n",
       "      <td>32907536.0</td>\n",
       "      <td>32907536.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>20</th>\n",
       "      <td>13</td>\n",
       "      <td>32911888.0</td>\n",
       "      <td>32911888.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>23</th>\n",
       "      <td>13</td>\n",
       "      <td>32915005.0</td>\n",
       "      <td>32915005.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>24</th>\n",
       "      <td>13</td>\n",
       "      <td>32915411.0</td>\n",
       "      <td>32915414.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>26</th>\n",
       "      <td>13</td>\n",
       "      <td>32919637.0</td>\n",
       "      <td>32919637.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>27</th>\n",
       "      <td>13</td>\n",
       "      <td>32920016.0</td>\n",
       "      <td>32920016.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>28</th>\n",
       "      <td>13</td>\n",
       "      <td>32920618.0</td>\n",
       "      <td>32920618.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>29</th>\n",
       "      <td>13</td>\n",
       "      <td>32920844.0</td>\n",
       "      <td>32920844.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>30</th>\n",
       "      <td>13</td>\n",
       "      <td>32929058.0</td>\n",
       "      <td>32929059.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>31</th>\n",
       "      <td>13</td>\n",
       "      <td>32929232.0</td>\n",
       "      <td>32929232.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>32</th>\n",
       "      <td>13</td>\n",
       "      <td>32929387.0</td>\n",
       "      <td>32929387.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>33</th>\n",
       "      <td>13</td>\n",
       "      <td>32930373.0</td>\n",
       "      <td>32930373.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>34</th>\n",
       "      <td>13</td>\n",
       "      <td>32936646.0</td>\n",
       "      <td>32936646.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>37</th>\n",
       "      <td>13</td>\n",
       "      <td>32951115.0</td>\n",
       "      <td>32951115.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>39</th>\n",
       "      <td>13</td>\n",
       "      <td>32954561.0</td>\n",
       "      <td>32954561.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>40</th>\n",
       "      <td>13</td>\n",
       "      <td>32954692.0</td>\n",
       "      <td>32954692.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>41</th>\n",
       "      <td>13</td>\n",
       "      <td>32954741.0</td>\n",
       "      <td>32954741.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>43</th>\n",
       "      <td>13</td>\n",
       "      <td>32968607.0</td>\n",
       "      <td>32968607.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>44</th>\n",
       "      <td>13</td>\n",
       "      <td>32970736.0</td>\n",
       "      <td>32970736.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>45</th>\n",
       "      <td>13</td>\n",
       "      <td>32971425.0</td>\n",
       "      <td>32971425.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>46</th>\n",
       "      <td>13</td>\n",
       "      <td>32973012.0</td>\n",
       "      <td>32973012.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>47</th>\n",
       "      <td>16</td>\n",
       "      <td>23615043.0</td>\n",
       "      <td>23615043.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>48</th>\n",
       "      <td>16</td>\n",
       "      <td>23640160.0</td>\n",
       "      <td>23640160.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>49</th>\n",
       "      <td>16</td>\n",
       "      <td>68770847.0</td>\n",
       "      <td>68770847.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>51</th>\n",
       "      <td>16</td>\n",
       "      <td>68771372.0</td>\n",
       "      <td>68771372.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>53</th>\n",
       "      <td>16</td>\n",
       "      <td>68771547.0</td>\n",
       "      <td>68771547.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>54</th>\n",
       "      <td>16</td>\n",
       "      <td>68772681.0</td>\n",
       "      <td>68772681.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>55</th>\n",
       "      <td>16</td>\n",
       "      <td>68820717.0</td>\n",
       "      <td>68820717.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>57</th>\n",
       "      <td>16</td>\n",
       "      <td>68847692.0</td>\n",
       "      <td>68847692.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>58</th>\n",
       "      <td>16</td>\n",
       "      <td>68847694.0</td>\n",
       "      <td>68847694.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>60</th>\n",
       "      <td>16</td>\n",
       "      <td>68847719.0</td>\n",
       "      <td>68847719.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>61</th>\n",
       "      <td>16</td>\n",
       "      <td>68847726.0</td>\n",
       "      <td>68847726.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>62</th>\n",
       "      <td>16</td>\n",
       "      <td>68847727.0</td>\n",
       "      <td>68847727.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>63</th>\n",
       "      <td>16</td>\n",
       "      <td>68855852.0</td>\n",
       "      <td>68855852.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>64</th>\n",
       "      <td>16</td>\n",
       "      <td>68856474.0</td>\n",
       "      <td>68856474.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>66</th>\n",
       "      <td>16</td>\n",
       "      <td>68857792.0</td>\n",
       "      <td>68857792.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>67</th>\n",
       "      <td>16</td>\n",
       "      <td>68867612.0</td>\n",
       "      <td>68867612.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>68</th>\n",
       "      <td>17</td>\n",
       "      <td>7576265.0</td>\n",
       "      <td>7576265.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>70</th>\n",
       "      <td>17</td>\n",
       "      <td>7577679.0</td>\n",
       "      <td>7577679.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>71</th>\n",
       "      <td>17</td>\n",
       "      <td>7578115.0</td>\n",
       "      <td>7578115.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>72</th>\n",
       "      <td>17</td>\n",
       "      <td>7578645.0</td>\n",
       "      <td>7578645.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>74</th>\n",
       "      <td>17</td>\n",
       "      <td>7578712.0</td>\n",
       "      <td>7578714.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>75</th>\n",
       "      <td>17</td>\n",
       "      <td>7578837.0</td>\n",
       "      <td>7578837.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>76</th>\n",
       "      <td>17</td>\n",
       "      <td>7579472.0</td>\n",
       "      <td>7579472.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>78</th>\n",
       "      <td>17</td>\n",
       "      <td>7579801.0</td>\n",
       "      <td>7579801.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>80</th>\n",
       "      <td>17</td>\n",
       "      <td>7590601.0</td>\n",
       "      <td>7590601.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>82</th>\n",
       "      <td>17</td>\n",
       "      <td>41196822.0</td>\n",
       "      <td>41196824.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>83</th>\n",
       "      <td>17</td>\n",
       "      <td>41197940.0</td>\n",
       "      <td>41197940.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>84</th>\n",
       "      <td>17</td>\n",
       "      <td>41247621.0</td>\n",
       "      <td>41247621.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>88</th>\n",
       "      <td>19</td>\n",
       "      <td>1206797.0</td>\n",
       "      <td>1206797.0</td>\n",
       "    </tr>\n",
       "    <tr>\n",
       "      <th>91</th>\n",
       "      <td>19</td>\n",
       "      <td>1221418.0</td>\n",
       "      <td>1221418.0</td>\n",
       "    </tr>\n",
       "  </tbody>\n",
       "</table>\n",
       "</div>"
      ],
      "text/plain": [
       "    Chr       Start         End\n",
       "1    10  89623142.0  89623142.0\n",
       "3    10  89623901.0  89623901.0\n",
       "5    10  89654190.0  89654190.0\n",
       "7    10  89718057.0  89718057.0\n",
       "10   10  89720907.0  89720907.0\n",
       "12   10  89725294.0  89725294.0\n",
       "13   10  89725582.0  89725582.0\n",
       "14   13  32889968.0  32889968.0\n",
       "17   13  32905220.0  32905220.0\n",
       "19   13  32907536.0  32907536.0\n",
       "20   13  32911888.0  32911888.0\n",
       "23   13  32915005.0  32915005.0\n",
       "24   13  32915411.0  32915414.0\n",
       "26   13  32919637.0  32919637.0\n",
       "27   13  32920016.0  32920016.0\n",
       "28   13  32920618.0  32920618.0\n",
       "29   13  32920844.0  32920844.0\n",
       "30   13  32929058.0  32929059.0\n",
       "31   13  32929232.0  32929232.0\n",
       "32   13  32929387.0  32929387.0\n",
       "33   13  32930373.0  32930373.0\n",
       "34   13  32936646.0  32936646.0\n",
       "37   13  32951115.0  32951115.0\n",
       "39   13  32954561.0  32954561.0\n",
       "40   13  32954692.0  32954692.0\n",
       "41   13  32954741.0  32954741.0\n",
       "43   13  32968607.0  32968607.0\n",
       "44   13  32970736.0  32970736.0\n",
       "45   13  32971425.0  32971425.0\n",
       "46   13  32973012.0  32973012.0\n",
       "47   16  23615043.0  23615043.0\n",
       "48   16  23640160.0  23640160.0\n",
       "49   16  68770847.0  68770847.0\n",
       "51   16  68771372.0  68771372.0\n",
       "53   16  68771547.0  68771547.0\n",
       "54   16  68772681.0  68772681.0\n",
       "55   16  68820717.0  68820717.0\n",
       "57   16  68847692.0  68847692.0\n",
       "58   16  68847694.0  68847694.0\n",
       "60   16  68847719.0  68847719.0\n",
       "61   16  68847726.0  68847726.0\n",
       "62   16  68847727.0  68847727.0\n",
       "63   16  68855852.0  68855852.0\n",
       "64   16  68856474.0  68856474.0\n",
       "66   16  68857792.0  68857792.0\n",
       "67   16  68867612.0  68867612.0\n",
       "68   17   7576265.0   7576265.0\n",
       "70   17   7577679.0   7577679.0\n",
       "71   17   7578115.0   7578115.0\n",
       "72   17   7578645.0   7578645.0\n",
       "74   17   7578712.0   7578714.0\n",
       "75   17   7578837.0   7578837.0\n",
       "76   17   7579472.0   7579472.0\n",
       "78   17   7579801.0   7579801.0\n",
       "80   17   7590601.0   7590601.0\n",
       "82   17  41196822.0  41196824.0\n",
       "83   17  41197940.0  41197940.0\n",
       "84   17  41247621.0  41247621.0\n",
       "88   19   1206797.0   1206797.0\n",
       "91   19   1221418.0   1221418.0"
      ]
     },
     "execution_count": 111,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "ex.replace(['chr10', 'chr13', 'chr16', 'chr17', 'chr19'], [10, 13, 16, 17, 19])"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": "<div>\n<style scoped>\n    .dataframe tbody tr th:only-of-type {\n        vertical-align: middle;\n    }\n\n    .dataframe tbody tr th {\n        vertical-align: top;\n    }\n\n    .dataframe thead th {\n        text-align: right;\n    }\n</style>\n<table border=\"1\" class=\"dataframe\">\n  <thead>\n    <tr style=\"text-align: right;\">\n      <th></th>\n      <th>chave1</th>\n      <th>chave2</th>\n      <th>data1</th>\n      <th>data2</th>\n    </tr>\n  </thead>\n  <tbody>\n    <tr>\n      <th>0</th>\n      <td>a</td>\n      <td>um</td>\n      <td>0.665078</td>\n      <td>-2.217535</td>\n    </tr>\n    <tr>\n      <th>1</th>\n      <td>a</td>\n      <td>dois</td>\n      <td>1.793492</td>\n      <td>0.260864</td>\n    </tr>\n    <tr>\n      <th>2</th>\n      <td>b</td>\n      <td>um</td>\n      <td>-1.029816</td>\n      <td>1.835246</td>\n    </tr>\n    <tr>\n      <th>3</th>\n      <td>b</td>\n      <td>dois</td>\n      <td>-0.105318</td>\n      <td>0.573794</td>\n    </tr>\n    <tr>\n      <th>4</th>\n      <td>a</td>\n      <td>um</td>\n      <td>-2.292867</td>\n      <td>1.089472</td>\n    </tr>\n  </tbody>\n</table>\n</div>",
      "text/plain": "  chave1 chave2     data1     data2\n0      a     um  0.665078 -2.217535\n1      a   dois  1.793492  0.260864\n2      b     um -1.029816  1.835246\n3      b   dois -0.105318  0.573794\n4      a     um -2.292867  1.089472"
     },
     "execution_count": 5,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "import numpy as np\n",
    "agrupamento_df = pd.DataFrame({'chave1' : ['a', 'a', 'b', 'b', 'a'],\n",
    "                   'chave2' : ['um', 'dois', 'um', 'dois', 'um'],\n",
    "                   'data1' : np.random.randn(5),\n",
    "                   'data2' : np.random.randn(5)})\n",
    "agrupamento_df"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 13,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": "pandas.core.groupby.generic.SeriesGroupBy"
     },
     "execution_count": 13,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "agrupamento_chave1 = agrupamento_df['data1'].groupby(agrupamento_df['chave1'])\n",
    "type(agrupamento_chave1)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "Apesar do resultado da operação `groupby` não ser de fato um `DataFrame` podemos computar funções de agregação no resultado da mesma forma. Dentro do pandas as operações mais comuns em agrupamentos (máximo, mínimo, media etc.) foram desenvolvidas de forma otimizada e podem ser aplicadas diretamente ao nosso objeto `pandas.core.groupby.generic.SeriesGroupBy`.\n",
    "\n",
    "| **Nome da Função**                                                                                                                                               | **Descrição**                            |\n",
    "|------------------------------------------------------------------------------------------------------------------------------------------------------------------|------------------------------------------|\n",
    "| [`count`](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.core.groupby.DataFrameGroupBy.count.html?pandas.core.groupby.DataFrameGroupBy.count) |  Número de valores no agrupamento        |\n",
    "| [`sum`](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.core.groupby.GroupBy.sum.html?pandas.core.groupby.GroupBy.sum)                         | Soma dos valores no agrupamento          |\n",
    "| [`mean`](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.core.groupby.GroupBy.mean.html?pandas.core.groupby.GroupBy.mean)                      | Media dos valores no agrupamento         |\n",
    "| [`median`](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.core.groupby.GroupBy.median.html?pandas.core.groupby.GroupBy.median)                | Mediana dos valores no agrupamento       |\n",
    "| [`std`](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.core.groupby.GroupBy.std.html?pandas.core.groupby.GroupBy.std)                         | Desvio padrão dos valores no agrupamento |\n",
    "| [`var`](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.core.groupby.GroupBy.var.html?pandas.core.groupby.GroupBy.var)                         | Variância dos valores no agrupamento     |\n",
    "| [`min`](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.core.groupby.GroupBy.min.html?pandas.core.groupby.GroupBy.min)                         | Menores valores no agrupamento           |\n",
    "| [`max`](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.core.groupby.GroupBy.max.html?pandas.core.groupby.GroupBy.max)                         | Maiores valores no agrupamento           |\n",
    "| [`prod`](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.core.groupby.GroupBy.prod.html?pandas.core.groupby.GroupBy.prod)                      | Produto dos valores no agrupamento       |\n",
    "| [`first`](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.core.groupby.GroupBy.first.html?pandas.core.groupby.GroupBy.first)                   | Primeiros valores no agrupamento         |\n",
    "| [`last`](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.core.groupby.GroupBy.last.html?pandas.core.groupby.GroupBy.last)                      | Últimos valores no agrupamento           |"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 27,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": "<div>\n<style scoped>\n    .dataframe tbody tr th:only-of-type {\n        vertical-align: middle;\n    }\n\n    .dataframe tbody tr th {\n        vertical-align: top;\n    }\n\n    .dataframe thead th {\n        text-align: right;\n    }\n</style>\n<table border=\"1\" class=\"dataframe\">\n  <thead>\n    <tr style=\"text-align: right;\">\n      <th></th>\n      <th>count</th>\n      <th>mean</th>\n      <th>std</th>\n      <th>min</th>\n      <th>25%</th>\n      <th>50%</th>\n      <th>75%</th>\n      <th>max</th>\n    </tr>\n    <tr>\n      <th>chave1</th>\n      <th></th>\n      <th></th>\n      <th></th>\n      <th></th>\n      <th></th>\n      <th></th>\n      <th></th>\n      <th></th>\n    </tr>\n  </thead>\n  <tbody>\n    <tr>\n      <th>a</th>\n      <td>3.0</td>\n      <td>0.055234</td>\n      <td>2.110335</td>\n      <td>-2.292867</td>\n      <td>-0.813895</td>\n      <td>0.665078</td>\n      <td>1.229285</td>\n      <td>1.793492</td>\n    </tr>\n    <tr>\n      <th>b</th>\n      <td>2.0</td>\n      <td>-0.567567</td>\n      <td>0.653718</td>\n      <td>-1.029816</td>\n      <td>-0.798691</td>\n      <td>-0.567567</td>\n      <td>-0.336443</td>\n      <td>-0.105318</td>\n    </tr>\n  </tbody>\n</table>\n</div>",
      "text/plain": "        count      mean       std       min       25%       50%       75%  \\\nchave1                                                                      \na         3.0  0.055234  2.110335 -2.292867 -0.813895  0.665078  1.229285   \nb         2.0 -0.567567  0.653718 -1.029816 -0.798691 -0.567567 -0.336443   \n\n             max  \nchave1            \na       1.793492  \nb      -0.105318  "
     },
     "execution_count": 27,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "descricoes_chave1 = agrupamento_chave1.describe()\n",
    "descricoes_chave1"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "Podemos desenvolver nossas próprias funções de agregação e usá-las no nosso agrupamento com o auxílio da função [`agg`](https://pandas.pydata.org/pandas-docs/stable/reference/api/pandas.core.groupby.GroupBy.agg.html?pandas.core.groupby.GroupBy.agg)."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 29,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": "chave1\na    4.086360\nb    0.924497\nName: data1, dtype: float64"
     },
     "execution_count": 29,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "def pico_a_pico(arr):\n",
    "    return arr.max() - arr.min()\n",
    "\n",
    "\n",
    "agrupamento_chave1.agg(pico_a_pico)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "Em vários casos é necessário computador diferentes funções para o mesmo agrupamento e analisar em conjunto os resultados. Nesse cenário, podemos passar uma lista de funções como parâmetro para a função `agg`. Nesse caso, nos é retornado um `DataFrame` com o resultado das computações no agrupamento."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 32,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": "<div>\n<style scoped>\n    .dataframe tbody tr th:only-of-type {\n        vertical-align: middle;\n    }\n\n    .dataframe tbody tr th {\n        vertical-align: top;\n    }\n\n    .dataframe thead th {\n        text-align: right;\n    }\n</style>\n<table border=\"1\" class=\"dataframe\">\n  <thead>\n    <tr style=\"text-align: right;\">\n      <th></th>\n      <th>sum</th>\n      <th>count</th>\n      <th>mean</th>\n      <th>pico_a_pico</th>\n    </tr>\n    <tr>\n      <th>chave1</th>\n      <th></th>\n      <th></th>\n      <th></th>\n      <th></th>\n    </tr>\n  </thead>\n  <tbody>\n    <tr>\n      <th>a</th>\n      <td>0.165703</td>\n      <td>3</td>\n      <td>0.055234</td>\n      <td>4.086360</td>\n    </tr>\n    <tr>\n      <th>b</th>\n      <td>-1.135134</td>\n      <td>2</td>\n      <td>-0.567567</td>\n      <td>0.924497</td>\n    </tr>\n  </tbody>\n</table>\n</div>",
      "text/plain": "             sum  count      mean  pico_a_pico\nchave1                                        \na       0.165703      3  0.055234     4.086360\nb      -1.135134      2 -0.567567     0.924497"
     },
     "execution_count": 32,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "resultado_agrupamentos_df = agrupamento_chave1.agg([\"sum\", \"count\", \"mean\", pico_a_pico])\n",
    "type(resultado_agrupamentos_df)"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "Não precisamos usar como nome das colunas o que é retornado para nós usando a sintaxe acima. Caso seja necessário dar nomes específicos as colunas resultantes das operações no agrupamento."
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 38,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/html": "<div>\n<style scoped>\n    .dataframe tbody tr th:only-of-type {\n        vertical-align: middle;\n    }\n\n    .dataframe tbody tr th {\n        vertical-align: top;\n    }\n\n    .dataframe thead th {\n        text-align: right;\n    }\n</style>\n<table border=\"1\" class=\"dataframe\">\n  <thead>\n    <tr style=\"text-align: right;\">\n      <th></th>\n      <th>soma</th>\n      <th>contagem</th>\n      <th>media</th>\n      <th>pico a pico</th>\n    </tr>\n    <tr>\n      <th>chave1</th>\n      <th></th>\n      <th></th>\n      <th></th>\n      <th></th>\n    </tr>\n  </thead>\n  <tbody>\n    <tr>\n      <th>a</th>\n      <td>0.165703</td>\n      <td>3</td>\n      <td>0.055234</td>\n      <td>4.086360</td>\n    </tr>\n    <tr>\n      <th>b</th>\n      <td>-1.135134</td>\n      <td>2</td>\n      <td>-0.567567</td>\n      <td>0.924497</td>\n    </tr>\n  </tbody>\n</table>\n</div>",
      "text/plain": "            soma  contagem     media  pico a pico\nchave1                                           \na       0.165703         3  0.055234     4.086360\nb      -1.135134         2 -0.567567     0.924497"
     },
     "execution_count": 38,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "resultado_agrupamentos_df = agrupamento_chave1.agg([(\"soma\", \"sum\"), (\"contagem\", \"count\"), (\"media\", \"mean\"), (\"pico a pico\", pico_a_pico)])\n",
    "resultado_agrupamentos_df"
   ]
  }
 ],
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   "name": "workshop-python_pandas_gnmk.ipynb",
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  "kernelspec": {
   "display_name": "Python 3",
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