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fails at pyhmmer #357

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dcampbdc opened this issue Aug 21, 2024 · 0 comments
Open

fails at pyhmmer #357

dcampbdc opened this issue Aug 21, 2024 · 0 comments
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bug Something isn't working

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@dcampbdc
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  • pharokka version: 1.6.1
  • Python version: 3.12.2
  • Operating System: Linux Mint 21.3

Hi! I'm trying to run pharokka.py on a contig and it always gets stuck in the same place after starting to run pyhmmer. None of my other contigs from the dataset return this error.

$ pharokka.py -t 2 -d ~/pharokka_DB/ --skip_mash -i 8p5_11B_NODE_62_length_2252_cov_44.925878.fna -o 8p5_11B_NODE_62_length_2252_cov_44.925878_pharokka -f

Traceback (most recent call last):
File "/home/ddawg/miniconda/bin/pharokka.py", line 499, in
main()
File "/home/ddawg/miniconda/bin/pharokka.py", line 364, in main
best_results_pyhmmer = run_pyhmmer(
^^^^^^^^^^^^
File "/home/ddawg/miniconda/bin/hmm.py", line 34, in run_pyhmmer
with pyhmmer.easel.SequenceFile(
^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "pyhmmer/easel.pyx", line 6289, in pyhmmer.easel.SequenceFile.init
File "pyhmmer/easel.pyx", line 6283, in pyhmmer.easel.SequenceFile.init
ValueError: Could not determine alphabet of file: '8p5_11B_NODE_62_length_2252_cov_44.925878_pharokka/phanotate_aas_tmp.fasta'

Input fasta: 8p5_11B_NODE_62_length_2252_cov_44.925878.fna.txt

@gbouras13 gbouras13 added the bug Something isn't working label Oct 7, 2024
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