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7. Read Processing & Mapping
George Pacheco edited this page Jul 27, 2021
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We trim and map all samples using PaleoMix--v1.2.5. For the GBS samples, we used the same
.yaml
file used above, the only difference being that now we used the filtered.fastq
files.
xsbatch -c XXX --mem-per-cpu XXX -J GBS --time XXX -- bam_pipeline run --jre-option "-XmxXXXg" --max-threads XXX --bwa-max-threads XXX --adapterremoval-max-threads XXX --destination ~/data/Pigeons/PBGP/Analyses/PaleoMix_GBS/ ~/data/Pigeons/PBGP/PBGP--Analyses/PBGP--Final_PaleoMix_GBS.yaml
xsbatch -c XXX --mem-per-cpu XXX -J Re-Seq --time XXX -- bam_pipeline run --jre-option "-XmxXXXg" --max-threads XXX --bwa-max-threads XXX --adapterremoval-max-threads XXX --destination ~/data/Pigeons/Analysis/PaleoMix_Re-Sequencing/ ~/data/Pigeons/Analysis/FPGP--Final_PaleoMix_Re-Sequencing.yaml
- 1. Data Access
- 2. Sequencing Quality Check
- 3. Demultiplexing
- 4. Creation of Mapping Targets
- 5. Filtering For Chimeric Reads
- 6. GBS Sexing
- 7. Read Processing & Mapping
- 8. Running Stats & Filtering of Bad Samples
- 9. Filtering of Possible Paralogs
- 10. Merging of Duplicate Cases
- 11. Investigation of Filtering of Possible Paralogs
- 12. Creation of Specific Datasets
- 13. Loci Information
- 14. Heterozygosity Calculation
- 15. Population Genetics Statistics
- 16. Phylogenetic Reconstruction
- 17. Multidimensional Scaling
- 18. Estimation of Individual Ancestries
- 19. Inference of Population Splits
- 20. Measuring of Linkage Disequilibrium
- 21. GWAS