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When reverseComplement=true is passed to BwaAlnInteractive, I think the negative flag should be flipped, but not the cigar. The cigar is always relative to the top strand of the genome. I have confirmed this by mapping two reads, both reverse complements of each other, and the mapping information only differs on the strand, but not the cigar, even in the XA tag.
This occurs here and here when building the Hits. Both places call the Hit.apply method, which will reverse the cigar.
When
reverseComplement=true
is passed toBwaAlnInteractive
, I think thenegative
flag should be flipped, but not the cigar. The cigar is always relative to the top strand of the genome. I have confirmed this by mapping two reads, both reverse complements of each other, and the mapping information only differs on the strand, but not the cigar, even in theXA
tag.This occurs here and here when building the
Hit
s. Both places call theHit.apply
method, which will reverse the cigar.The text was updated successfully, but these errors were encountered: