You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Hi PlantTribes!
I have been using the PlanTribes to perform gene clustering and have successfully run the AssemblyPostProcess and GeneFamilyClassifier. However, I fail to get the RAxML tree from the PhylogenomicsAnalysis (legacy pipeline).
This is the code I used >> /home/mpsud1/cn78_scratch/mpsud1/Ortho_Cluster_Gene/PlantTribes-master/legacy/PhylogenomicsAnalysis --orthogroup_faa geneFamilyClassification_dir/orthogroups_fasta --scaffold 12Gv1.0 --method orthomcl --pasta_alignments --tree_inference raxml --max_memory 65536 --num_threads 20
I think the alignment was performed successfully? I kept getting the following warnings and the error for RAxML:
Refused to clean '/home/mpsud1/.pasta/pastajob/tempbrvrc6cr/init_aln': not created by PASTA
'/home/mpsud1/.pasta/pastajob/tempbrvrc6cr/init_aln' is not registered as a temporary directory that was created by this process!
Refused to clean '/home/mpsud1/.pasta/pastajob/temp1a0ifb4g/init_aln': not created by PASTA
'/home/mpsud1/.pasta/pastajob/temp1a0ifb4g/init_aln' is not registered as a temporary directory that was created by this process!
Refused to clean '/home/mpsud1/.pasta/pastajob/tempa9hn4uoa/init_aln': not created by PASTA
'/home/mpsud1/.pasta/pastajob/tempa9hn4uoa/init_aln' is not registered as a temporary directory that was created by this process!
mv: cannot stat 'RAxML_bipartitions.': No such file or directory
rm: cannot remove 'RAxML_bestTree.': No such file or directory
rm: cannot remove 'RAxML_bipartitionsBranchLabels.': No such file or directory
rm: cannot remove 'RAxML_bootstrap.': No such file or directory
rm: cannot remove 'RAxML_info.*': No such file or directory
What I did was I ran the GeneFamilyPhylogeny pipeline using the alignment files I got from PhylogenomicsAnalysis (legacy pipeline). It seems to be working for now. Would that be okay? Also, how do I confirm that I have got all the alignments?
This is the command I used >> /home/mpsud1/cn78_scratch/mpsud1/Ortho_Cluster_Gene/PlantTribes-master/pipelines/GeneFamilyPhylogenyBuilder --orthogroup_aln phylogenomicsAnalysis_dir/orthogroups_aln --scaffold 12Gv1.0 --method orthomcl --tree_inference raxml --num_threads 20
Thank you in advance! I hope to hear from you soon.
The text was updated successfully, but these errors were encountered:
Hi PlantTribes!
I have been using the PlanTribes to perform gene clustering and have successfully run the AssemblyPostProcess and GeneFamilyClassifier. However, I fail to get the RAxML tree from the PhylogenomicsAnalysis (legacy pipeline).
This is the code I used >> /home/mpsud1/cn78_scratch/mpsud1/Ortho_Cluster_Gene/PlantTribes-master/legacy/PhylogenomicsAnalysis --orthogroup_faa geneFamilyClassification_dir/orthogroups_fasta --scaffold 12Gv1.0 --method orthomcl --pasta_alignments --tree_inference raxml --max_memory 65536 --num_threads 20
I think the alignment was performed successfully? I kept getting the following warnings and the error for RAxML:
Refused to clean '/home/mpsud1/.pasta/pastajob/tempbrvrc6cr/init_aln': not created by PASTA
'/home/mpsud1/.pasta/pastajob/tempbrvrc6cr/init_aln' is not registered as a temporary directory that was created by this process!
Refused to clean '/home/mpsud1/.pasta/pastajob/temp1a0ifb4g/init_aln': not created by PASTA
'/home/mpsud1/.pasta/pastajob/temp1a0ifb4g/init_aln' is not registered as a temporary directory that was created by this process!
Refused to clean '/home/mpsud1/.pasta/pastajob/tempa9hn4uoa/init_aln': not created by PASTA
'/home/mpsud1/.pasta/pastajob/tempa9hn4uoa/init_aln' is not registered as a temporary directory that was created by this process!
mv: cannot stat 'RAxML_bipartitions.': No such file or directory
rm: cannot remove 'RAxML_bestTree.': No such file or directory
rm: cannot remove 'RAxML_bipartitionsBranchLabels.': No such file or directory
rm: cannot remove 'RAxML_bootstrap.': No such file or directory
rm: cannot remove 'RAxML_info.*': No such file or directory
What I did was I ran the GeneFamilyPhylogeny pipeline using the alignment files I got from PhylogenomicsAnalysis (legacy pipeline). It seems to be working for now. Would that be okay? Also, how do I confirm that I have got all the alignments?
This is the command I used >> /home/mpsud1/cn78_scratch/mpsud1/Ortho_Cluster_Gene/PlantTribes-master/pipelines/GeneFamilyPhylogenyBuilder --orthogroup_aln phylogenomicsAnalysis_dir/orthogroups_aln --scaffold 12Gv1.0 --method orthomcl --tree_inference raxml --num_threads 20
Thank you in advance! I hope to hear from you soon.
The text was updated successfully, but these errors were encountered: