lecture_1.ipynb

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   "cell_type": "code",
   "execution_count": 1,
   "metadata": {},
   "outputs": [],
   "source": [
    "# 1. imports \n",
    "\n",
    "# pip install biotite plotly nbformat \n",
    "\n",
    "import plotly.express as px \n",
    "from biotite.sequence.io.fasta import FastaFile"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 2,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      ">tr|A0A0Q0WE68|A0A0Q0WE68_9EURY acylphosphatase OS=Methanosaeta sp. SDB OX=1735328 GN=APR56_14035 PE=3 SV=1\n",
      "MKRVDIIASGDVQKVGFRDVVQKIGRDLGLSGTVENREPYDVRIVAEGEEDGLKEFIEAL\n",
      "KIKRGPIHVRELEVSWSEATGEFPYFKILRGDWQEELGERFDVAVGLLYRSIEIGEENLA\n",
      "LGRENLALGKENLAVSKENLAIGKKMLEKQDTMIERQDETIGEIRGMRSDFQDHMEKRFT\n",
      "KIEGEIAEIKAAIADIKGNA\n",
      ">tr|A0A1S3CGZ8|A0A1S3CGZ8_CUCME acylphosphatase OS=Cucumis melo OX=3656 GN=LOC103500820 PE=3 SV=1\n",
      "MASASSSSSAAFAVGPLIRNRSTRFLPCRNLHHKWSFKDAGSHSRLSLFNYYNSSPLLPS\n",
      "LPIRHRLLLPHPPLHLLLRSRSPSFRYPLPLMASTVPHQAGPETPQSNPTKTVRVVIKGR\n",
      "VQGVFYRDWTVENATELGLKGWVRNRRDGSVEALFSGRPESVTEMEQRCRRGPPAAMVTG\n",
      "FQVFPSSDDPGPGFERLRTA\n"
     ]
    }
   ],
   "source": [
    "# 2. input file \n",
    "\n",
    "# uniprot \"acylphosphatase family > with active site annotation > length less than 200 > to fasta\"\n",
    "\n",
    "# look at the file on disk \n",
    "\n",
    "input_file = \"./fasta/raw.fa\"\n",
    "\n",
    "! head \"$input_file\""
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 8,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Read 28856 sequences\n"
     ]
    },
    {
     "data": {
      "text/plain": [
       "['MKRVDIIASGDVQKVGFRDVVQKIGRDLGLSGTVENREPYDVRIVAEGEEDGLKEFIEALKIKRGPIHVRELEVSWSEATGEFPYFKILRGDWQEELGERFDVAVGLLYRSIEIGEENLALGRENLALGKENLAVSKENLAIGKKMLEKQDTMIERQDETIGEIRGMRSDFQDHMEKRFTKIEGEIAEIKAAIADIKGNA',\n",
       " 'MASASSSSSAAFAVGPLIRNRSTRFLPCRNLHHKWSFKDAGSHSRLSLFNYYNSSPLLPSLPIRHRLLLPHPPLHLLLRSRSPSFRYPLPLMASTVPHQAGPETPQSNPTKTVRVVIKGRVQGVFYRDWTVENATELGLKGWVRNRRDGSVEALFSGRPESVTEMEQRCRRGPPAAMVTGFQVFPSSDDPGPGFERLRTA']"
      ]
     },
     "execution_count": 8,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "# 3. save the FASTA headers, the sequences, and the sequence lengths \n",
    "\n",
    "fn = FastaFile.read(input_file)\n",
    "\n",
    "headers = []\n",
    "sequences = []\n",
    "lengths = []\n",
    "\n",
    "for header, sequence in fn.items():\n",
    "    \n",
    "    header = header.split(\"|\")[1]\n",
    "    sequence = str(sequence)\n",
    "    length = len(sequence)\n",
    "    \n",
    "    headers.append(header)\n",
    "    sequences.append(sequence)\n",
    "    lengths.append(length)\n",
    "\n",
    "print(f\"Read {len(headers)} sequences\")\n",
    "\n",
    "sequences[:2]"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 4,
   "metadata": {},
   "outputs": [
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   ],
   "source": [
    "# 4. examine the length distribution \n",
    "\n",
    "px.histogram(x=lengths, width=1024)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 5,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Selected 26779 of 28856 sequences\n"
     ]
    }
   ],
   "source": [
    "# 5. construct a new FASTA with just the sequences we want \n",
    "\n",
    "output_fasta = FastaFile() \n",
    "count = 0 \n",
    "\n",
    "for header, sequence in zip(headers, sequences):\n",
    "    if 64 < len(sequence) < 128:\n",
    "        if sequence.startswith(\"M\"):\n",
    "            if not \"X\" in sequence:\n",
    "                count += 1\n",
    "                output_fasta[header] = sequence\n",
    "\n",
    "print(f\"Selected {count} of {len(sequences)} sequences\")\n",
    "\n",
    "output_fasta.write(\"fasta/hypf.fa\")"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 6,
   "metadata": {},
   "outputs": [],
   "source": [
    "# examine sequences bioinformatically \n",
    "\n",
    "# Alignment? \n",
    "\n",
    "# Embedding? (can we learn a good embedding?)\n",
    "\n",
    "# Examine predicted structures in PyMOL "
   ]
  }
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