Quantify tissue-scale stresses along AP, ML and DV axis of Zebrafish embryo?

[elena-scarpa]

Hi Johannes,
Just wanted to confirm that we'd like to measure forces tissue-scale level, as per fig3 in the Gross et al Biorxiv paper. I'd probably need some help with data interpretation when I have them.
I am running some reconstructions on the droplets that I have (I have also control ones injected in agarose) and they look good. when I come back from holidays I will try injecting again and using a confocal for imaging to see if anything improves reconstruction-wise. I'll be in touch later in July, in the meantime thank you so much for your help!

[jo-mueller]

Hi @elena-scarpa ,

thanks for opening the issue! One thing I found recently on the reconstruction (shame on me for never actually trying it) is that increasing the smoothing sigma parameter to slightly higher values (2 or 3) improves the droplet reconstruction big time. It probably has a notable impact on measured cell-scale forces, but tissue stresses should more or less be unaffected by it.