Toffy tiling top left corner not accurate #273
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I have noticed that in MIBIcontrol the target area selected and the target area registered after left click never match up. Not sure if this is contributing to the issue. I tried this several times, and the end result is identical (I was pretty careful not to move the mouse while clicking on it) After mouse click: |
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Got it, I would reach out to ionpath in that case to get the double cursor issue resolved. Once the MIBIControl coregistration is updated, the toffy coregistration will also need to be updated in order to stay current. |
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Sounds good. Thanks for your help! |
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On the same topic, if I've generated a tiled_json, when MIBIcontrol's co-registration was poor, then had the co-registration corrected by Ionpath, and subsequently re-registered optical-stage in Toffy, can I use the same tiled_json, or do I need to generate another tiled_json. I'm guessing that since the json uses stage coordinates the previous tiled_json should still work. Am I thinking about that the right way? |
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I'm not sure, it depends on what changes when ionpath changes their coregistration. I don't know which set of values get updated. Sorry! |
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No worries. I gave it a go, and will update once I have the result. |
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I've been trying tiling using Toffy. However, I can't seem to get the top left corner for the first panel to reflect the area that I picked in MIBIcontrol as the top left corner. I have tried recalibrating stage to optical coordinates and that hasn't worked. I've attached an image of the preview tiling area generated in Toffy. The red dot indicates the target coordinates selected in MIBIcontrol. Does the target area selected in MIBI control translate as the centroid of the top left corner FOV in Toffy? If that is the case, then the two are misregistered by about 600 microns in x and 200 microns in y (FOV is 400). I'm likely doing something wrong, and ideas as to what that might be?
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