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config.yaml
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config.yaml
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# list of sample names as used by genpipes: ["sample1", "sample2"]
SAMPLES: []
# list marks as used by genpipes: ["H3K27me3", "H3K27ac"]
MARKS: []
# path to genpipes output folder
GENPIPES: "/lustre06/project/6004736/alvann/from_narval/220408_SJG2_A29P/ChIP/out/01A_genpipes_chipseq"
# path to output folder
OUT_PATH: ""
CPU: 10
CPU_COMPUTE_MATRIX: 10
# path to black list file
BLACK_LIST: "/lustre06/project/6004736/alvann/from_narval/REFERENCES/annotation_files/ENCODE.blacklist.wgEncodeHg19ConsensusSignalArtifactRegions.hg19.noOverlaps.bed"
# bin size to use for bigwig summary
BIN_SIZE: [5000]
# bed files used for bigwig summary
SUMMARY_REGIONS:
gene.ens.hg19: "/lustre06/project/6004736/alvann/from_narval/REFERENCES/annotation_files/Ensembl.ensGene.whole.hg19.collapsed.can.bed"
promoter5kb.ens.hg 19: "/lustre06/project/6004736/alvann/from_narval/REFERENCES/annotation_files/Ensembl.ensGene.whole.hg19.collapsed.promoter5kb.can.bed"
cgi10kb.UCSC.hg19: "/lustre06/project/6004736/alvann/from_narval/REFERENCES/annotation_files/UCSC.regulation.CpGIslands.cpgIslandExt.hg19.10kb.bed"
# PCs to compute
PC:
pc12: [1, 2]
pc34: [3, 4]
# normalization method: RPKM, CPM etc (see deeptools for options)
NORM_METHOD: ["CPM"]
# scale factrs to use (numeric or rx)
SCALE_FACTOR: [1, "rx"]
# if SCALE_FACTOR = "rx" provide path to csv file with rx scale factors for each sample
# file needs to have column Sample and column rx
SCALE_FACTOR_FILE_RX: ""
# params to run compute matrix (used as input for heatmaps etc)
# change <name> to meaningful matrix name
COMPUT_MATRIX_PARAM:
<name>:
type: "scale"
ref_point: ""
region_bed: "Ensembl.ensGene.whole.hg19.collapsed.bed"
length: 10000
up: 2500
down: 2500
samples: ["", "", ""]
<name>:
type: "ref_point"
ref_point: "center"
region_bed: "Ensembl.ensGene.whole.hg19.collapsed.promoter5kb.bed"
length: ""
up: 2500
down: 2500
samples: ["", "", ""]
# params plot heatmap and plot profile. See deeptols for options.
PLOT_HEATMAP_PARAM:
name:
color: "#1e5385,#f8f79d,#cc2200"
colorLine:
plotType: se
samples: ""
lables: ""
startLabel: ""
endLabel: ""
title: ""
height: 15
kmeans: 2
PLOT_PROFILE_PARAM:
name:
lables: ""
startLabel: ""
endLabel: ""
title: ""
plotType: se
colors:
# samples used when calculating Z-score
Z_SCORE_PARAM:
control: [""]
condition:
condition1: [""]
condition2: [""]