diff --git a/checks/class-directories-macosx-check.php b/checks/class-directories-macosx-check.php
new file mode 100644
index 00000000..f56b9113
--- /dev/null
+++ b/checks/class-directories-macosx-check.php
@@ -0,0 +1,65 @@
+<?php
+/**
+ * Check if Macosx directory is included
+ *
+ * @package Theme Check
+ */
+
+/**
+ * Check if Macosx directory is included.
+ *
+ * Check if Macosx directory is included. If it is, require them to be removed.
+ */
+class Directories_Macosx_Check implements themecheck {
+	/**
+	 * Error messages, warnings and info notices.
+	 *
+	 * @var array $error
+	 */
+	protected $error = array();
+
+	/**
+	 * Check that return true for good/okay/acceptable, false for bad/not-okay/unacceptable.
+	 *
+	 * @param array $php_files File paths and content for PHP files.
+	 * @param array $css_files File paths and content for CSS files.
+	 * @param array $other_files Folder names, file paths and content for other files.
+	 */
+	public function check( $php_files, $css_files, $other_files ) {
+
+		$excluded_directories = array(
+			'__MACOSX',
+		);
+
+		$ret = true;
+
+		$path        = get_template_directory();
+		$directories = scandir( $path );
+
+		foreach ( $directories as $dir ) {
+			checkcount();
+
+			if ( in_array( $dir, $excluded_directories, true ) ) {
+				$this->error[] = sprintf(
+					'<span class="tc-lead tc-required">%s</span>: %s',
+					__( 'REQUIRED', 'theme-check' ),
+					__( 'Please remove any extraneous directories like <strong>.git</strong>, <strong>.svn</strong> or <strong>__MACOSX</strong> from the ZIP file before uploading it. For the <a href="https://make.wordpress.org/themes/handbook/review/required/#9-files">List of disallowed files</a>', 'theme-check' )
+				);
+				$ret           = false;
+			}
+		}
+
+		return $ret;
+	}
+
+	/**
+	 * Get error messages from the checks.
+	 *
+	 * @return array Error message.
+	 */
+	public function getError() {
+		return $this->error;
+	}
+}
+
+$themechecks[] = new Directories_Macosx_Check();