diff --git a/packages/block-editor/src/components/block-preview/index.js b/packages/block-editor/src/components/block-preview/index.js
index cf0937507be095..0fb7f55b9955d2 100644
--- a/packages/block-editor/src/components/block-preview/index.js
+++ b/packages/block-editor/src/components/block-preview/index.js
@@ -16,6 +16,7 @@ import deprecated from '@wordpress/deprecated';
  */
 import { ExperimentalBlockEditorProvider } from '../provider';
 import AutoHeightBlockPreview from './auto';
+import EditorStyles from '../editor-styles';
 import { store as blockEditorStore } from '../../store';
 import { BlockListItems } from '../block-list';
 
@@ -113,7 +114,11 @@ export function useBlockPreview( { blocks, props = {}, layout } ) {
 		[]
 	);
 	const settings = useMemo(
-		() => ( { ...originalSettings, __unstableIsPreviewMode: true } ),
+		() => ( {
+			...originalSettings,
+			styles: undefined, // Clear styles included by the parent settings, as they are already output by the parent's EditorStyles.
+			__unstableIsPreviewMode: true,
+		} ),
 		[ originalSettings ]
 	);
 	const disabledRef = useDisabled();
@@ -128,6 +133,7 @@ export function useBlockPreview( { blocks, props = {}, layout } ) {
 			value={ renderedBlocks }
 			settings={ settings }
 		>
+			<EditorStyles />
 			<BlockListItems renderAppender={ false } layout={ layout } />
 		</ExperimentalBlockEditorProvider>
 	);
diff --git a/packages/block-editor/src/hooks/duotone.js b/packages/block-editor/src/hooks/duotone.js
index 2bbe18793fe558..39a8979782a0c6 100644
--- a/packages/block-editor/src/hooks/duotone.js
+++ b/packages/block-editor/src/hooks/duotone.js
@@ -37,11 +37,22 @@ import { scopeSelector } from '../components/global-styles/utils';
 import { useBlockSettings } from './utils';
 import { default as StylesFiltersPanel } from '../components/global-styles/filters-panel';
 import { useBlockEditingMode } from '../components/block-editing-mode';
+import { __unstableUseBlockElement as useBlockElement } from '../components/block-list/use-block-props/use-block-refs';
 import { store as blockEditorStore } from '../store';
 import { unlock } from '../lock-unlock';
 
 const EMPTY_ARRAY = [];
 
+// Safari does not always update the duotone filter when the duotone colors
+// are changed. This browser check is later used to force a re-render of the block
+// element to ensure the duotone filter is updated. The check is included at the
+// root of this file as it only needs to be run once per page load.
+const isSafari =
+	window?.navigator.userAgent &&
+	window.navigator.userAgent.includes( 'Safari' ) &&
+	! window.navigator.userAgent.includes( 'Chrome' ) &&
+	! window.navigator.userAgent.includes( 'Chromium' );
+
 extend( [ namesPlugin ] );
 
 function useMultiOriginPresets( { presetSetting, defaultSetting } ) {
@@ -223,6 +234,7 @@ const withDuotoneControls = createHigherOrderComponent(
 );
 
 function DuotoneStyles( {
+	clientId,
 	id: filterId,
 	selector: duotoneSelector,
 	attribute: duotoneAttr,
@@ -278,6 +290,8 @@ function DuotoneStyles( {
 		useDispatch( blockEditorStore )
 	);
 
+	const blockElement = useBlockElement( clientId );
+
 	useEffect( () => {
 		if ( ! isValidFilter ) return;
 
@@ -294,12 +308,30 @@ function DuotoneStyles( {
 			__unstableType: 'svgs',
 		} );
 
+		// Safari does not always update the duotone filter when the duotone colors
+		// are changed. When using Safari, force the block element to be repainted by
+		// the browser to ensure any changes are reflected visually. This logic matches
+		// that used on the site frontend in `block-supports/duotone.php`.
+		if ( blockElement && isSafari ) {
+			const display = blockElement.style.display;
+			// Switch to `inline-block` to force a repaint. In the editor, `inline-block`
+			// is used instead of `none` to ensure that scroll position is not affected,
+			// as `none` results in the editor scrolling to the top of the block.
+			blockElement.style.display = 'inline-block';
+			// Simply accessing el.offsetHeight flushes layout and style
+			// changes in WebKit without having to wait for setTimeout.
+			// eslint-disable-next-line no-unused-expressions
+			blockElement.offsetHeight;
+			blockElement.style.display = display;
+		}
+
 		return () => {
 			deleteStyleOverride( filterId );
 			deleteStyleOverride( `duotone-${ filterId }` );
 		};
 	}, [
 		isValidFilter,
+		blockElement,
 		colors,
 		selector,
 		filterId,
@@ -378,6 +410,7 @@ const withDuotoneStyles = createHigherOrderComponent(
 			<>
 				{ shouldRender && (
 					<DuotoneStyles
+						clientId={ props.clientId }
 						id={ filterClass }
 						selector={ selector }
 						attribute={ attribute }