- Removes network and subnetwork parameters because CloudOS now sets this automatically (#315).
- Allows for better control of Lifebit memory resourses (#317).
- Fixed a bug in the post processing that changed the order of the Bamlist (#322)
- Forces usage of NXF_VER=20.04.1 on Sumner in main.pbs to circumvent that the pipeline is written in DSL = 1 (NF changed default language) (#326).
- rMATs
4.1.1
->4.1.2
(#316)
- removed fasta file requirement from
Gen3-DRS
option (#297) - The
google-lifesciences
has been defined as the executor when google profile is used. (#305) GTEX
downlod option has been removed due to being obsolete (#299)Gen3-DRS
was renamed toGTEX
as it's now the only way to download GTEX file (#299)- Added a new
--manifest
parameter with the input being the .json manifest file downloaded from GTEX (#304) - Added
--reads
parameter which receives a CSV file with samples for which the analysis will be limited to when usingGTEX
(#304)
- Fix pipeline crach when using
Gen3-DRS
input due toenv(...)
format being used in process output (#294) - Changed
gtex
input to support new manifest file format (#296)
- None performed
- None added
- Adds saving of all the process .command* log files to results/process-logs folder (#251)
- Adds pipeline workdir
--cleanup
option to clear all intermediate files on pipeline successful completion (true by default, false for CloudOS) (#238, #284, 089d6e3) - Adds pipeline
--error_strategy
parameter to be able to specify pipeline error strategy directly from command line (doesn't work if specified in config linked by-c
or-config
nextflow params) (#267) - Parametrizes google executor parameters so that pipeline can now be run on different CloudOS environments (#281)
- Adds a new
--download_from
optionFTP
mode to download SRA samples from EBI FTP (#283) - Adds new parameter
--save_unmapped
that makes saving of STAR unmapped files optional (false by default) (#284)
- Adds missing trimmomatic logs to the multiqc report (#244)
- Implemented correct support for input strandness in star process when
--stranded
issecond-strand
(was hardcoded tostrType=2
and only supportedfirst-strand
orfalse
before) (#264) - Issue that stringti_merged results folder as well as some other folders are missing all or some files (#263)
- Fix pipeline crash when
params.stranded
was set tofalse
(#276) - Fixes old parameters in google.config that were undesirably overwriting nextflow.config parameters on CloudOS (#281, 217e202)
- Updates the following tools: (#248)
- STAR
2.7.3
->2.7.9a
NOTE: Requires a new index! (updated in test profile) - Samtools
1.10
->1.13
- StringTie
2.1.3b
->2.1.7
- Gffread
0.11.7
->0.12.7
- multiqc
1.8
->1.11
- deeptools
3.4.0
->3.5.1
- bioconductor-rtracklayer
1.46.0
->1.52.0
- gffcompare
0.11.2
->0.12.6
- bedtools
2.29.2
->2.30.0
- sra-tools
2.10.8
->2.11.0
- pigz
2.3.4
->2.6.0
- gdc-client
1.5.0
->1.6.1
- STAR
- Moves all containers to https://hub.docker.com/u/anczukowlab
- Considerably reduces number of basic redundant CI tests by removing completely the
max_retries
matrix andpush
fromon: [push, pull_request]
- Adds CI test for sra-downloading pipeline pathway (only supported with docker profile for now) (#253)