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Quick start examples exit with error (-U missing) and give different numbers #45
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Hi Stefan, Thank you for the thorough testing. I will have a look at what is going on here. regards |
Hey Stefan, Thanks, |
Also, @scogi , do you mind letting me know how many sequences you have in Thanks, |
Hi Ryan, test_r941/raconx3_medakax1/consensus_raconx3_medakax1.fa: 6 sequences i have not been able to locate the file longread_umi_version_dump.txt. Where should it be saved? Thanks, |
@ziels test_r941/raconx3_medakax1/consensus_raconx3_medakax1.fa: 6 sequences test_r10/raconx2_medakax1/consensus_raconx3_medakax1.fa: 90 sequences Content of longread_umi_version_dump.txt: |
Hi,
fantastic tool, promises to solve a lot of our problems!
I managed to install it (Ubuntu 18.04, conda installation as proposed in #36), but if I run the Quickstart examples for Nanopore data R9.4.1 and R10, i get an error message that the "-U" parameter is missing
-U is missing but required. Exiting.
I thus modified the commands to:
longread_umi nanopore_pipeline -d test_reads.fq -v 30 -o test_r941 -s 90 -e 90 -m 3500 -M 6000 -f CAAGCAGAAGACGGCATACGAGAT -F AGRGTTYGATYMTGGCTCAG -r AATGATACGGCGACCACCGAGATC -R CGACATCGAGGTGCCAAAC -c 3 -p 1 -q r941_min_high_g330 -t 1 -U r941_min_high_g360
and
longread_umi nanopore_pipeline -d ont_r10_zymo_rrna.fq -o test_r10 -v 25 -q r10_min_high_g340 -m 3500 -M 6000 -s 90 -e 90 -f CAAGCAGAAGACGGCATACGAGAT -F AGRGTTYGATYMTGGCTCAG -r AATGATACGGCGACCACCGAGATC -R CGACATCGAGGTGCCAAAC -c 2 -p 2 -t 1 -U r103_min_high_g360
which works.
However, the numbers in variants.fq and consensus_raconx3_medakax1.fa differ from what the Quick start section states
R9.4.1
variants.fq: 1 (should be 3, according to quick start section)
consensus_raconx3_medakax1.fa: 6 (should be 9)
R10
variants.fq: 12 sequences (should be 13)
consensus_raconx3_medakax1.fa 88 (should be 98)
My questions:
Thank you,
Stefan
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