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GUI.py
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import tkinter as tk
from System import System
from PIL import ImageTk, Image
from KeyPad import KeyPad
import os
from Camera import Camera
from CamAdjustFrame import CamAdjustFrame
from multiprocessing import Queue
from queue import Empty
from threading import Thread
class GUI:
def __init__(self):
#used to set memory requirement for Camera
os.system("sudo echo 0|sudo tee /sys/module/usbcore/parameters/usbfs_memory_mb")
'''This class configures and populates the toplevel window.
self.root is the toplevel containing window.'''
_bgcolor = '#d9d9d9' # X11 color: 'gray85'
_fgcolor = '#000000' # X11 color: 'black'
_compcolor = '#d9d9d9' # X11 color: 'gray85'
_ana1color = '#d9d9d9' # X11 color: 'gray85'
_ana2color = '#ececec' # Closest X11 color: 'gray92'
font25 = "-family {Segoe UI} -size 25 -weight bold -slant roman " \
"-underline 0 -overstrike 0"
font20 = "-family {Segoe UI} -size 20 -slant roman " \
"-underline 0 -overstrike 0"
self.system = System(self) #system object that stores all objects for al functionality (gets passed the GUI)
self.queue = Queue()#queue used for processing instructions from threads for updating GUI etc.
#carousel zeroed flag and counters and keypad flag
self.carouselZeroed = False
self.kpRunning = 0
#counters to track whether button click opens or closes camera adjust window and on or off LED
self.camAdjustCnt=0
self.ledPowerCnt = 0
#top-level configuration(window position, Title, Background color)
self.root = tk.Tk()
self.root.geometry("1920x1060+-1+-2")
self.root.title("Blood Analyzer")
self.root.configure(background="#d9d9d9")
#configuration for health/ status frame which contains status (busy or ready) and camera temp (celsius)
self.healthStatusFrame = tk.LabelFrame(self.root)
self.healthStatusFrame.place(relx=0.006, rely=0.0, relheight=0.16
, relwidth=0.192)
self.healthStatusFrame.configure(relief='groove')
self.healthStatusFrame.configure(borderwidth="8")
self.healthStatusFrame.configure(font=font25)
self.healthStatusFrame.configure(foreground="black")
self.healthStatusFrame.configure(text='''Health/Status''')
self.healthStatusFrame.configure(background="#d9d9d9")
self.statusText = tk.Text(self.healthStatusFrame)
self.statusText.place(relx=0.062, rely=0.275, relheight=0.282, relwidth=0.622
, bordermode='ignore')
self.statusText.configure(background="white")
self.statusText.configure(font=font20)
self.statusText.configure(foreground="black")
self.statusText.configure(highlightbackground="#d9d9d9")
self.statusText.configure(highlightcolor="black")
self.statusText.configure(insertbackground="black")
self.statusText.configure(selectbackground="#c4c4c4")
self.statusText.configure(selectforeground="black")
self.statusText.configure(wrap="word")
self.statusText.insert(tk.END,"Ready")
self.statusText.configure(highlightbackground="green")
self.statusText.configure(highlightthickness=6)
self.camTempText = tk.Text(self.healthStatusFrame)
self.camTempText.place(relx=0.064, rely=0.61, relheight=0.282
, relwidth=0.3, bordermode='ignore')
self.camTempText.configure(background="white")
self.camTempText.configure(font=font20)
self.camTempText.configure(foreground="black")
self.camTempText.configure(highlightbackground="#d9d9d9")
self.camTempText.configure(highlightcolor="black")
self.camTempText.configure(insertbackground="black")
self.camTempText.configure(selectbackground="#c4c4c4")
self.camTempText.configure(selectforeground="black")
self.camTempText.configure(wrap="word")
self.camTempLabel = tk.Label(self.healthStatusFrame)
self.camTempLabel.place(relx=0.39, rely=0.688, height=26, width=210
, bordermode='ignore')
self.camTempLabel.configure(font=font20)
self.camTempLabel.configure(background="#d9d9d9")
self.camTempLabel.configure(disabledforeground="#a3a3a3")
self.camTempLabel.configure(foreground="#000000")
self.camTempLabel.configure(text='''Cam Temp (°C)''')
#configuration for the camera Frame which contains camera adjust button
self.cameraFrame = tk.LabelFrame(self.root)
self.cameraFrame.place(relx=0.202, rely=0.39, relheight=0.12
, relwidth=0.192)
self.cameraFrame.configure(relief='groove')
self.cameraFrame.configure(borderwidth="8")
self.cameraFrame.configure(font=font25)
self.cameraFrame.configure(foreground="black")
self.cameraFrame.configure(text='''Camera''')
self.cameraFrame.configure(background="#d9d9d9")
self.cameraFrame.configure(highlightbackground="#d9d9d9")
self.cameraFrame.configure(highlightcolor="black")
self.camAdjustButton = tk.Button(self.cameraFrame)
self.camAdjustButton.place(relx=0.062, rely=0.308, height=64, width=300
, bordermode='ignore')
self.camAdjustButton.configure(activebackground="#ececec")
self.camAdjustButton.configure(activeforeground="#000000")
self.camAdjustButton.configure(background="#b7b7b7")
self.camAdjustButton.configure(disabledforeground="#a3a3a3")
self.camAdjustButton.configure(foreground="#000000")
self.camAdjustButton.configure(highlightbackground="#d9d9d9")
self.camAdjustButton.configure(highlightcolor="black")
self.camAdjustButton.configure(pady="0")
self.camAdjustButton.configure(text='''Camera Adjust''')
self.camAdjustButton.configure(font=font20)
self.camAdjustButton.configure(command = self.camAdjust) #callback for adjust button is self.camAdjust()
#configuration for the Focus Frame which contains manual focus (+) (-) buttons, display for curPos, video button
# and auto focus button
self.focusFrame = tk.LabelFrame(self.root)
self.focusFrame.place(relx=0.202, rely=0.0, relheight=0.391
, relwidth=0.192)
self.focusFrame.configure(relief='groove')
self.focusFrame.configure(borderwidth="8")
self.focusFrame.configure(font=font25)
self.focusFrame.configure(foreground="black")
self.focusFrame.configure(text='''Focus''')
self.focusFrame.configure(background="#d9d9d9")
self.focusFrame.configure(highlightbackground="#d9d9d9")
self.focusFrame.configure(highlightcolor="black")
self.minusStepFocusButton = tk.Button(self.focusFrame)
self.minusStepFocusButton.place(relx=0.062, rely=0.114, height=64, width=150
, bordermode='ignore')
self.minusStepFocusButton.configure(activebackground="#ececec")
self.minusStepFocusButton.configure(activeforeground="#000000")
self.minusStepFocusButton.configure(background="#b7b7b7")
self.minusStepFocusButton.configure(disabledforeground="#a3a3a3")
self.minusStepFocusButton.configure(foreground="#000000")
self.minusStepFocusButton.configure(highlightbackground="#d9d9d9")
self.minusStepFocusButton.configure(highlightcolor="black")
self.minusStepFocusButton.configure(pady="0")
self.minusStepFocusButton.configure(text='''Step(-)''')
self.minusStepFocusButton.configure(command= self.jogDwn)#callback for manual step(-) is self.jogDwn()
self.minusStepFocusButton.configure(font=font20)
self.plusStepFocusButton = tk.Button(self.focusFrame)
self.plusStepFocusButton.place(relx=0.497, rely=0.114, height=64, width=150
, bordermode='ignore')
self.plusStepFocusButton.configure(activebackground="#ececec")
self.plusStepFocusButton.configure(activeforeground="#000000")
self.plusStepFocusButton.configure(background="#b7b7b7")
self.plusStepFocusButton.configure(disabledforeground="#a3a3a3")
self.plusStepFocusButton.configure(foreground="#000000")
self.plusStepFocusButton.configure(highlightbackground="#d9d9d9")
self.plusStepFocusButton.configure(highlightcolor="black")
self.plusStepFocusButton.configure(pady="0")
self.plusStepFocusButton.configure(text='''Step(+)''')
self.plusStepFocusButton.configure(command= self.jogUp)#callback for manual step(+) is self.jogUp()
self.plusStepFocusButton.configure(font=font20)
self.focusPosText = tk.Text(self.focusFrame)
self.focusPosText.place(relx=0.062, rely=0.343, relheight=0.137
, relwidth=0.273, bordermode='ignore')
self.focusPosText.configure(background="white")
self.focusPosText.configure(font=font20)
self.focusPosText.configure(foreground="black")
self.focusPosText.configure(highlightbackground="#d9d9d9")
self.focusPosText.configure(highlightcolor="black")
self.focusPosText.configure(insertbackground="black")
self.focusPosText.configure(selectbackground="#c4c4c4")
self.focusPosText.configure(selectforeground="black")
self.focusPosText.configure(wrap="word")
self.focusPosText.insert(tk.END,"10000")
self.focusPosLabel = tk.Label(self.focusFrame)
self.focusPosLabel.place(relx=0.352, rely=0.375, height=26, width=209
, bordermode='ignore')
self.focusPosLabel.configure(activebackground="#f9f9f9")
self.focusPosLabel.configure(activeforeground="black")
self.focusPosLabel.configure(background="#d9d9d9")
self.focusPosLabel.configure(disabledforeground="#a3a3a3")
self.focusPosLabel.configure(foreground="#000000")
self.focusPosLabel.configure(highlightbackground="#d9d9d9")
self.focusPosLabel.configure(highlightcolor="black")
self.focusPosLabel.configure(text='''Focus pos (um)''')
self.focusPosLabel.configure(font=font20)
self.videoButton = tk.Button(self.focusFrame)
self.videoButton.place(relx=0.062, rely=0.543, height=64, width=300
, bordermode='ignore')
self.videoButton.configure(activebackground="#ececec")
self.videoButton.configure(activeforeground="#000000")
self.videoButton.configure(background="#b7b7b7")
self.videoButton.configure(disabledforeground="#a3a3a3")
self.videoButton.configure(foreground="#000000")
self.videoButton.configure(highlightbackground="#d9d9d9")
self.videoButton.configure(highlightcolor="black")
self.videoButton.configure(pady="0")
self.videoButton.configure(text='''Video''')
self.videoButton.configure(font=font20)
self.videoButton.configure(command=self.videoPower)#callback for video button is self.videoPower()
self.autoFocusButton = tk.Button(self.focusFrame)
self.autoFocusButton.place(relx=0.062, rely=0.743, height=64, width=300
, bordermode='ignore')
self.autoFocusButton.configure(activebackground="#ececec")
self.autoFocusButton.configure(activeforeground="#000000")
self.autoFocusButton.configure(background="#b7b7b7")
self.autoFocusButton.configure(disabledforeground="#a3a3a3")
self.autoFocusButton.configure(foreground="#000000")
self.autoFocusButton.configure(highlightbackground="#d9d9d9")
self.autoFocusButton.configure(highlightcolor="black")
self.autoFocusButton.configure(pady="0")
self.autoFocusButton.configure(text='''Auto Focus''')
self.autoFocusButton.configure(command = self.autoFocus) #threaded and hooked to e-stop
self.autoFocusButton.configure(font=font20)
#configuration for manual frame which contains capture image button, analyze sample button, and save data button
self.manualFrame = tk.LabelFrame(self.root)
self.manualFrame.place(relx=0.396, rely=0.649, relheight=0.342
, relwidth=0.192)
self.manualFrame.configure(relief='groove')
self.manualFrame.configure(borderwidth="8")
self.manualFrame.configure(font=font25)
self.manualFrame.configure(foreground="black")
self.manualFrame.configure(text='''Manual''')
self.manualFrame.configure(background="#d9d9d9")
self.manualFrame.configure(highlightbackground="#d9d9d9")
self.manualFrame.configure(highlightcolor="black")
self.captureImageButton = tk.Button(self.manualFrame)
self.captureImageButton.place(relx=0.062, rely=0.131, height=64, width=300
, bordermode='ignore')
self.captureImageButton.configure(activebackground="#ececec")
self.captureImageButton.configure(activeforeground="#000000")
self.captureImageButton.configure(background="#b7b7b7")
self.captureImageButton.configure(disabledforeground="#a3a3a3")
self.captureImageButton.configure(foreground="#000000")
self.captureImageButton.configure(highlightbackground="#d9d9d9")
self.captureImageButton.configure(highlightcolor="black")
self.captureImageButton.configure(pady="0")
self.captureImageButton.configure(text='''Capture image''')
self.captureImageButton.configure(font=font20)
self.captureImageButton.configure(command=self.showIm)#callback for capture image button is self.showIm()
self.analyzeSampleButton = tk.Button(self.manualFrame)
self.analyzeSampleButton.place(relx=0.062, rely=0.425, height=64, width=300
, bordermode='ignore')
self.analyzeSampleButton.configure(activebackground="#ececec")
self.analyzeSampleButton.configure(activeforeground="#000000")
self.analyzeSampleButton.configure(background="#b7b7b7")
self.analyzeSampleButton.configure(disabledforeground="#a3a3a3")
self.analyzeSampleButton.configure(foreground="#000000")
self.analyzeSampleButton.configure(highlightbackground="#d9d9d9")
self.analyzeSampleButton.configure(highlightcolor="black")
self.analyzeSampleButton.configure(pady="0")
self.analyzeSampleButton.configure(text='''Analyze sample''')
self.analyzeSampleButton.configure(command= self.countBlood)#callback for Analyze sample button is self.countBlood()
self.analyzeSampleButton.configure(font=font20)
self.saveDataButton = tk.Button(self.manualFrame)
self.saveDataButton.place(relx=0.062, rely=0.719, height=64, width=300
, bordermode='ignore')
self.saveDataButton.configure(activebackground="#ececec")
self.saveDataButton.configure(activeforeground="#000000")
self.saveDataButton.configure(background="#b7b7b7")
self.saveDataButton.configure(disabledforeground="#a3a3a3")
self.saveDataButton.configure(foreground="#000000")
self.saveDataButton.configure(highlightbackground="#d9d9d9")
self.saveDataButton.configure(highlightcolor="black")
self.saveDataButton.configure(pady="0")
self.saveDataButton.configure(text='''Save data''')
self.saveDataButton.configure(command = self.saveData)#callback for Save data button is self.saveData()
self.saveDataButton.configure(font=font20)
#configuration for utility frame which contains create file button, open file button, and set pathology interface
self.utilityFrame = tk.LabelFrame(self.root)
self.utilityFrame.place(relx=0.202, rely=0.515, relheight=0.47
, relwidth=0.192)
self.utilityFrame.configure(relief='groove')
self.utilityFrame.configure(borderwidth="8")
self.utilityFrame.configure(font=font25)
self.utilityFrame.configure(foreground="black")
self.utilityFrame.configure(text='''Utility''')
self.utilityFrame.configure(background="#d9d9d9")
self.utilityFrame.configure(highlightbackground="#d9d9d9")
self.utilityFrame.configure(highlightcolor="black")
self.createFileButton = tk.Button(self.utilityFrame)
self.createFileButton.place(relx=0.062, rely=0.095, height=64, width=300
, bordermode='ignore')
self.createFileButton.configure(activebackground="#ececec")
self.createFileButton.configure(activeforeground="#000000")
self.createFileButton.configure(background="#b7b7b7")
self.createFileButton.configure(disabledforeground="#a3a3a3")
self.createFileButton.configure(foreground="#000000")
self.createFileButton.configure(highlightbackground="#d9d9d9")
self.createFileButton.configure(highlightcolor="black")
self.createFileButton.configure(pady="0")
self.createFileButton.configure(text='''Create file''')
self.createFileButton.configure(command= self.createFile)#callback for create file button is self.createFile()
self.createFileButton.configure(font=font20)
self.openFileButton = tk.Button(self.utilityFrame)
self.openFileButton.place(relx=0.062, rely=0.31, height=64, width=300
, bordermode='ignore')
self.openFileButton.configure(activebackground="#ececec")
self.openFileButton.configure(activeforeground="#000000")
self.openFileButton.configure(background="#b7b7b7")
self.openFileButton.configure(disabledforeground="#a3a3a3")
self.openFileButton.configure(foreground="#000000")
self.openFileButton.configure(highlightbackground="#d9d9d9")
self.openFileButton.configure(highlightcolor="black")
self.openFileButton.configure(pady="0")
self.openFileButton.configure(text='''Open file''')
self.openFileButton.configure(command = self.openFile)#callback for open file button is self.openFile()
self.openFileButton.configure(font=font20)
self.setPathologyButton = tk.Button(self.utilityFrame)
self.setPathologyButton.place(relx=0.062, rely=0.524, height=64, width=300
, bordermode='ignore')
self.setPathologyButton.configure(activebackground="#ececec")
self.setPathologyButton.configure(activeforeground="#000000")
self.setPathologyButton.configure(background="#b7b7b7")
self.setPathologyButton.configure(disabledforeground="#a3a3a3")
self.setPathologyButton.configure(foreground="#000000")
self.setPathologyButton.configure(highlightbackground="#d9d9d9")
self.setPathologyButton.configure(highlightcolor="black")
self.setPathologyButton.configure(pady="0")
self.setPathologyButton.configure(text='''Set pathology''')
self.setPathologyButton.configure(command = self.setPathology)#callback for set pathology button is self.setPathology()
self.setPathologyButton.configure(font=font20)
self.minPathologyText = tk.Text(self.utilityFrame)
self.minPathologyText.place(relx=0.062, rely=0.786, relheight=0.152
, relwidth=0.335, bordermode='ignore')
self.minPathologyText.configure(background="white")
self.minPathologyText.configure(font=font20)
self.minPathologyText.configure(foreground="black")
self.minPathologyText.configure(highlightbackground="#d9d9d9")
self.minPathologyText.configure(highlightcolor="black")
self.minPathologyText.configure(insertbackground="black")
self.minPathologyText.configure(selectbackground="#c4c4c4")
self.minPathologyText.configure(selectforeground="black")
self.minPathologyText.configure(wrap="word")
self.minPathologyText.bind("<Button-1>",lambda e: self.genKeyPad(e))#callback for min patholgy text is self.genKeyPad(e)
self.maxPathologyText = tk.Text(self.utilityFrame)
self.maxPathologyText.place(relx=0.528, rely=0.786, relheight=0.152
, relwidth=0.335, bordermode='ignore')
self.maxPathologyText.configure(background="white")
self.maxPathologyText.configure(font=font20)
self.maxPathologyText.configure(foreground="black")
self.maxPathologyText.configure(highlightbackground="#d9d9d9")
self.maxPathologyText.configure(highlightcolor="black")
self.maxPathologyText.configure(insertbackground="black")
self.maxPathologyText.configure(selectbackground="#c4c4c4")
self.maxPathologyText.configure(selectforeground="black")
self.maxPathologyText.configure(wrap="word")
self.maxPathologyText.bind("<Button-1>",lambda e: self.genKeyPad(e))#callback for max patholgy text is self.genKeyPad(e)
self.minPathologyLabel = tk.Label(self.utilityFrame)
self.minPathologyLabel.place(relx=0.124, rely=0.714, height=25, width=70
, bordermode='ignore')
self.minPathologyLabel.configure(background="#d9d9d9")
self.minPathologyLabel.configure(disabledforeground="#a3a3a3")
self.minPathologyLabel.configure(foreground="#000000")
self.minPathologyLabel.configure(text='''Min''')
self.minPathologyLabel.configure(font=font20)
self.maxPathologyLabel = tk.Label(self.utilityFrame)
self.maxPathologyLabel.place(relx=0.559, rely=0.714, height=25, width=70
, bordermode='ignore')
self.maxPathologyLabel.configure(activebackground="#f9f9f9")
self.maxPathologyLabel.configure(activeforeground="black")
self.maxPathologyLabel.configure(background="#d9d9d9")
self.maxPathologyLabel.configure(disabledforeground="#a3a3a3")
self.maxPathologyLabel.configure(foreground="#000000")
self.maxPathologyLabel.configure(highlightbackground="#d9d9d9")
self.maxPathologyLabel.configure(highlightcolor="black")
self.maxPathologyLabel.configure(text='''Max''')
self.maxPathologyLabel.configure(font=font20)
#configuration for automatic frame which contains auto-start button and E-stop button
self.automaticFrame = tk.LabelFrame(self.root)
self.automaticFrame.place(relx=0.592, rely=0.649, relheight=0.342
, relwidth=0.192)
self.automaticFrame.configure(relief='groove')
self.automaticFrame.configure(borderwidth="8")
self.automaticFrame.configure(font=font25)
self.automaticFrame.configure(foreground="black")
self.automaticFrame.configure(text='''Automatic''')
self.automaticFrame.configure(background="#d9d9d9")
self.automaticFrame.configure(highlightbackground="#d9d9d9")
self.automaticFrame.configure(highlightcolor="black")
self.autoStartButton = tk.Button(self.automaticFrame)
self.autoStartButton.place(relx=0.062, rely=0.131, height=64, width=300
, bordermode='ignore')
self.autoStartButton.configure(activebackground="#ececec")
self.autoStartButton.configure(activeforeground="#000000")
self.autoStartButton.configure(background="#b7b7b7")
self.autoStartButton.configure(disabledforeground="#a3a3a3")
self.autoStartButton.configure(foreground="#000000")
self.autoStartButton.configure(highlightbackground="#d9d9d9")
self.autoStartButton.configure(highlightcolor="black")
self.autoStartButton.configure(pady="0")
self.autoStartButton.configure(text='''Start''')
self.autoStartButton.configure(command= self.autoStart)#callback for auto start buttton is self.autoStart()
self.autoStartButton.configure(font=font20)
self.emergencyStopButton = tk.Button(self.automaticFrame)
self.emergencyStopButton.place(relx=0.062, rely=0.49, height=160, width=300
, bordermode='ignore')
self.emergencyStopButton.configure(activebackground="#ececec")
self.emergencyStopButton.configure(activeforeground="#000000")
self.emergencyStopButton.configure(background="#ff0000")
self.emergencyStopButton.configure(disabledforeground="#a3a3a3")
self.emergencyStopButton.configure(font=font20)
self.emergencyStopButton.configure(foreground="#000000")
self.emergencyStopButton.configure(highlightbackground="#d9d9d9")
self.emergencyStopButton.configure(highlightcolor="black")
self.emergencyStopButton.configure(pady="0")
self.emergencyStopButton.configure(text='''Emergency stop''')
self.emergencyStopButton.configure(command= self.eStop)#callback for e-stop buttton is self.eStop()
#configuration for illuminator frame which contains input text boxes for RGB values, on/off button, and set button
self.illuminatorFrame = tk.LabelFrame(self.root)
self.illuminatorFrame.place(relx=0.006, rely=0.159, relheight=0.483
, relwidth=0.192)
self.illuminatorFrame.configure(relief='groove')
self.illuminatorFrame.configure(borderwidth="8")
self.illuminatorFrame.configure(font=font25)
self.illuminatorFrame.configure(foreground="black")
self.illuminatorFrame.configure(text='''Illuminator''')
self.illuminatorFrame.configure(background="#d9d9d9")
self.illuminatorFrame.configure(highlightbackground="#d9d9d9")
self.illuminatorFrame.configure(highlightcolor="black")
self.ledPowerButton = tk.Button(self.illuminatorFrame)
self.ledPowerButton.place(relx=0.062, rely=0.093, height=64, width=300
, bordermode='ignore')
self.ledPowerButton.configure(activebackground="#757575")
self.ledPowerButton.configure(activeforeground="white")
self.ledPowerButton.configure(activeforeground="#000000")
self.ledPowerButton.configure(background="#b7b7b7")
self.ledPowerButton.configure(disabledforeground="#a3a3a3")
self.ledPowerButton.configure(foreground="#000000")
self.ledPowerButton.configure(highlightbackground="#d9d9d9")
self.ledPowerButton.configure(highlightcolor="black")
self.ledPowerButton.configure(pady="0")
self.ledPowerButton.configure(text='''On/Off''')
self.ledPowerButton.configure(command= self.ledPower)#callback for led power buttton is self.ledPower()
self.ledPowerButton.configure(font=font20)
self.redLevelText = tk.Text(self.illuminatorFrame)
self.redLevelText.place(relx=0.062, rely=0.278, relheight=0.139
, relwidth=0.329, bordermode='ignore')
self.redLevelText.configure(background="white")
self.redLevelText.configure(font=font20)
self.redLevelText.configure(foreground="black")
self.redLevelText.configure(highlightbackground="#d9d9d9")
self.redLevelText.configure(highlightcolor="black")
self.redLevelText.configure(insertbackground="black")
self.redLevelText.configure(selectbackground="#c4c4c4")
self.redLevelText.configure(selectforeground="black")
self.redLevelText.configure(wrap="word")
self.redLevelText.bind("<Button-1>",lambda e: self.genKeyPad(e))#callback for red level text is self.genKeyPad(e)
self.greenLevelText = tk.Text(self.illuminatorFrame)
self.greenLevelText.place(relx=0.062, rely=0.463, relheight=0.139
, relwidth=0.329, bordermode='ignore')
self.greenLevelText.configure(background="white")
self.greenLevelText.configure(font=font20)
self.greenLevelText.configure(foreground="black")
self.greenLevelText.configure(highlightbackground="#d9d9d9")
self.greenLevelText.configure(highlightcolor="black")
self.greenLevelText.configure(insertbackground="black")
self.greenLevelText.configure(selectbackground="#c4c4c4")
self.greenLevelText.configure(selectforeground="black")
self.greenLevelText.configure(wrap="word")
self.greenLevelText.bind("<Button-1>",lambda e: self.genKeyPad(e))#callback for green level text is self.genKeyPad(e)
self.blueLevelText = tk.Text(self.illuminatorFrame)
self.blueLevelText.place(relx=0.062, rely=0.648, relheight=0.139
, relwidth=0.329, bordermode='ignore')
self.blueLevelText.configure(background="white")
self.blueLevelText.configure(font=font20)
self.blueLevelText.configure(foreground="black")
self.blueLevelText.configure(highlightbackground="#d9d9d9")
self.blueLevelText.configure(highlightcolor="black")
self.blueLevelText.configure(insertbackground="black")
self.blueLevelText.configure(selectbackground="#c4c4c4")
self.blueLevelText.configure(selectforeground="black")
self.blueLevelText.configure(wrap="word")
self.blueLevelText.bind("<Button-1>",lambda e: self.genKeyPad(e))#callback for blue level text is self.genKeyPad(e)
self.ledSetButton = tk.Button(self.illuminatorFrame)
self.ledSetButton.place(relx=0.062, rely=0.81, height=64, width=300
, bordermode='ignore')
self.ledSetButton.configure(activebackground="#ececec")
self.ledSetButton.configure(activeforeground="#000000")
self.ledSetButton.configure(background="#b7b7b7")
self.ledSetButton.configure(disabledforeground="#a3a3a3")
self.ledSetButton.configure(foreground="#000000")
self.ledSetButton.configure(highlightbackground="#d9d9d9")
self.ledSetButton.configure(highlightcolor="black")
self.ledSetButton.configure(pady="0")
self.ledSetButton.configure(text='''Set''')
self.ledSetButton.configure(command= self.setLed)#callback for set led buttton is self.setLed()
self.ledSetButton.configure(font=font20)
self.redLevelLabel = tk.Label(self.illuminatorFrame)
self.redLevelLabel.place(relx=0.4, rely=0.32, height=26, width=175
, bordermode='ignore')
self.redLevelLabel.configure(activebackground="#f9f9f9")
self.redLevelLabel.configure(activeforeground="black")
self.redLevelLabel.configure(background="#d9d9d9")
self.redLevelLabel.configure(disabledforeground="#a3a3a3")
self.redLevelLabel.configure(foreground="#000000")
self.redLevelLabel.configure(highlightbackground="#d9d9d9")
self.redLevelLabel.configure(highlightcolor="black")
self.redLevelLabel.configure(text='''Red(0-255)''')
self.redLevelLabel.configure(font=font20)
self.greenLevelLabel = tk.Label(self.illuminatorFrame)
self.greenLevelLabel.place(relx=0.41, rely=0.51, height=26, width=183
, bordermode='ignore')
self.greenLevelLabel.configure(activebackground="#f9f9f9")
self.greenLevelLabel.configure(activeforeground="black")
self.greenLevelLabel.configure(background="#d9d9d9")
self.greenLevelLabel.configure(disabledforeground="#a3a3a3")
self.greenLevelLabel.configure(foreground="#000000")
self.greenLevelLabel.configure(highlightbackground="#d9d9d9")
self.greenLevelLabel.configure(highlightcolor="black")
self.greenLevelLabel.configure(text='''Green(0-255)''')
self.greenLevelLabel.configure(font=font20)
self.blueLevelLabel = tk.Label(self.illuminatorFrame)
self.blueLevelLabel.place(relx=0.387, rely=0.698, height=26, width=180
, bordermode='ignore')
self.blueLevelLabel.configure(activebackground="#f9f9f9")
self.blueLevelLabel.configure(activeforeground="black")
self.blueLevelLabel.configure(background="#d9d9d9")
self.blueLevelLabel.configure(disabledforeground="#a3a3a3")
self.blueLevelLabel.configure(foreground="#000000")
self.blueLevelLabel.configure(highlightbackground="#d9d9d9")
self.blueLevelLabel.configure(highlightcolor="black")
self.blueLevelLabel.configure(justify='left')
self.blueLevelLabel.configure(text='''Blue(0-255)''')
self.blueLevelLabel.configure(font=font20)
#configuration for carousel frame which contains manual step forward and backward buttons,
#zero carousel button, and go to slide interface
self.carouselFrame = tk.LabelFrame(self.root)
self.carouselFrame.place(relx=0.006, rely=0.64, relheight=0.3452
, relwidth=0.192)
self.carouselFrame.configure(relief='groove')
self.carouselFrame.configure(borderwidth="8")
self.carouselFrame.configure(font=font25)
self.carouselFrame.configure(foreground="black")
self.carouselFrame.configure(text='''Carousel''')
self.carouselFrame.configure(background="#d9d9d9")
self.carouselFrame.configure(highlightbackground="#d9d9d9")
self.carouselFrame.configure(highlightcolor="black")
self.minusStepCarouselButton = tk.Button(self.carouselFrame)
self.minusStepCarouselButton.place(relx=0.062, rely=0.135, height=64, width=150
, bordermode='ignore')
self.minusStepCarouselButton.configure(activebackground="#ececec")
self.minusStepCarouselButton.configure(activeforeground="#000000")
self.minusStepCarouselButton.configure(background="#b7b7b7")
self.minusStepCarouselButton.configure(disabledforeground="#a3a3a3")
self.minusStepCarouselButton.configure(foreground="#000000")
self.minusStepCarouselButton.configure(highlightbackground="#d9d9d9")
self.minusStepCarouselButton.configure(highlightcolor="black")
self.minusStepCarouselButton.configure(pady="0")
self.minusStepCarouselButton.configure(text='''Step(-)''')
self.minusStepCarouselButton.configure(command= self.cwStep)#callback for single step minus is self.cwStep()
self.minusStepCarouselButton.configure(font=font20)
self.plusStepCarouselButton = tk.Button(self.carouselFrame)
self.plusStepCarouselButton.place(relx=0.497, rely=0.135, height=64, width=150
, bordermode='ignore')
self.plusStepCarouselButton.configure(activebackground="#ececec")
self.plusStepCarouselButton.configure(activeforeground="#000000")
self.plusStepCarouselButton.configure(background="#b7b7b7")
self.plusStepCarouselButton.configure(disabledforeground="#a3a3a3")
self.plusStepCarouselButton.configure(foreground="#000000")
self.plusStepCarouselButton.configure(highlightbackground="#d9d9d9")
self.plusStepCarouselButton.configure(highlightcolor="black")
self.plusStepCarouselButton.configure(pady="0")
self.plusStepCarouselButton.configure(text='''Step(+)''')
self.plusStepCarouselButton.configure(command= self.ccwStep)#callback for single step plus is self.ccwStep()
self.plusStepCarouselButton.configure(font=font20)
self.zeroCarouselButton = tk.Button(self.carouselFrame)
self.zeroCarouselButton.place(relx=0.062, rely=0.439, height=64, width=300
, bordermode='ignore')
self.zeroCarouselButton.configure(activebackground="#ececec")
self.zeroCarouselButton.configure(activeforeground="#ffffff")
self.zeroCarouselButton.configure(background="#b7b7b7")
self.zeroCarouselButton.configure(disabledforeground="#a3a3a3")
self.zeroCarouselButton.configure(foreground="#000000")
self.zeroCarouselButton.configure(highlightbackground="#d9d9d9")
self.zeroCarouselButton.configure(highlightcolor="#ffffff")
self.zeroCarouselButton.configure(pady="0")
self.zeroCarouselButton.configure(text='''Zero''')
self.zeroCarouselButton.configure(command= self.zeroCarousel)#callback for zero carousel button is self.zeroCarousel()
self.zeroCarouselButton.configure(font=font20)
self.goToSlideText = tk.Text(self.carouselFrame)
self.goToSlideText.place(relx=0.062, rely=0.709, relheight=0.203
, relwidth=0.329, bordermode='ignore')
self.goToSlideText.configure(background="white")
self.goToSlideText.configure(font=font20)
self.goToSlideText.configure(foreground="black")
self.goToSlideText.configure(highlightbackground="#d9d9d9")
self.goToSlideText.configure(highlightcolor="black")
self.goToSlideText.configure(insertbackground="black")
self.goToSlideText.configure(selectbackground="#c4c4c4")
self.goToSlideText.configure(selectforeground="black")
self.goToSlideText.configure(wrap="word")
self.goToSlideText.bind("<Button-1>",lambda e: self.genKeyPad(e))#callback for go to slide text is self.genKeyPad(e)
self.goToSlideButton = tk.Button(self.carouselFrame)
self.goToSlideButton.place(relx=0.435, rely=0.709, height=64, width=164
, bordermode='ignore')
self.goToSlideButton.configure(activebackground="#ececec")
self.goToSlideButton.configure(activeforeground="#000000")
self.goToSlideButton.configure(background="#b7b7b7")
self.goToSlideButton.configure(disabledforeground="#a3a3a3")
self.goToSlideButton.configure(foreground="#000000")
self.goToSlideButton.configure(highlightbackground="#d9d9d9")
self.goToSlideButton.configure(highlightcolor="black")
self.goToSlideButton.configure(pady="0")
self.goToSlideButton.configure(text='''Go to Slide''')
self.goToSlideButton.configure(command= self.goToSlide)#callback for go to slide button is self.goToSlide()
self.goToSlideButton.configure(font=font20)
#configuration for sample parameters frame which contains sample id, sample data, wbc/rbc ratio and wbc/rbc counts
self.sampleParamsFrame = tk.LabelFrame(self.root)
self.sampleParamsFrame.place(relx=0.787, rely=0.649, relheight=0.342
, relwidth=0.208)
self.sampleParamsFrame.configure(relief='groove')
self.sampleParamsFrame.configure(borderwidth="8")
self.sampleParamsFrame.configure(font=font25)
self.sampleParamsFrame.configure(foreground="black")
self.sampleParamsFrame.configure(text='''Sample Parameters''')
self.sampleParamsFrame.configure(background="#d9d9d9")
self.sampleParamsFrame.configure(highlightbackground="#d9d9d9")
self.sampleParamsFrame.configure(highlightcolor="black")
self.sampleIdText = tk.Text(self.sampleParamsFrame)
self.sampleIdText.place(relx=0.062, rely=0.131, relheight=0.157
, relwidth=0.335, bordermode='ignore')
self.sampleIdText.configure(background="white")
self.sampleIdText.configure(font=font20)
self.sampleIdText.configure(foreground="black")
self.sampleIdText.configure(highlightbackground="#d9d9d9")
self.sampleIdText.configure(highlightcolor="black")
self.sampleIdText.configure(insertbackground="black")
self.sampleIdText.configure(selectbackground="#c4c4c4")
self.sampleIdText.configure(selectforeground="black")
self.sampleIdText.configure(wrap="word")
self.sampleDateText = tk.Text(self.sampleParamsFrame)
self.sampleDateText.place(relx=0.062, rely=0.327, relheight=0.157
, relwidth=0.335, bordermode='ignore')
self.sampleDateText.configure(background="white")
self.sampleDateText.configure(font=font20)
self.sampleDateText.configure(foreground="black")
self.sampleDateText.configure(highlightbackground="#d9d9d9")
self.sampleDateText.configure(highlightcolor="black")
self.sampleDateText.configure(insertbackground="black")
self.sampleDateText.configure(selectbackground="#c4c4c4")
self.sampleDateText.configure(selectforeground="black")
self.sampleDateText.configure(wrap="word")
self.sampleRatioText = tk.Text(self.sampleParamsFrame)
self.sampleRatioText.place(relx=0.062, rely=0.523, relheight=0.157
, relwidth=0.335, bordermode='ignore')
self.sampleRatioText.configure(background="white")
self.sampleRatioText.configure(font=font20)
self.sampleRatioText.configure(foreground="black")
self.sampleRatioText.configure(highlightbackground="#d9d9d9")
self.sampleRatioText.configure(highlightcolor="black")
self.sampleRatioText.configure(insertbackground="black")
self.sampleRatioText.configure(selectbackground="#c4c4c4")
self.sampleRatioText.configure(selectforeground="black")
self.sampleRatioText.configure(wrap="word")
self.sampleIdLabel = tk.Label(self.sampleParamsFrame)
self.sampleIdLabel.place(relx=0.45, rely=0.171, height=24, width=140
, bordermode='ignore')
self.sampleIdLabel.configure(activebackground="#f9f9f9")
self.sampleIdLabel.configure(activeforeground="black")
self.sampleIdLabel.configure(background="#d9d9d9")
self.sampleIdLabel.configure(disabledforeground="#a3a3a3")
self.sampleIdLabel.configure(foreground="#000000")
self.sampleIdLabel.configure(highlightbackground="#d9d9d9")
self.sampleIdLabel.configure(highlightcolor="black")
self.sampleIdLabel.configure(text='''Sample ID''')
self.sampleIdLabel.configure(font=font20)
self.sampleDateLabel = tk.Label(self.sampleParamsFrame)
self.sampleDateLabel.place(relx=0.4, rely=0.377, height=24, width=100
, bordermode='ignore')
self.sampleDateLabel.configure(activebackground="#f9f9f9")
self.sampleDateLabel.configure(activeforeground="black")
self.sampleDateLabel.configure(background="#d9d9d9")
self.sampleDateLabel.configure(disabledforeground="#a3a3a3")
self.sampleDateLabel.configure(foreground="#000000")
self.sampleDateLabel.configure(highlightbackground="#d9d9d9")
self.sampleDateLabel.configure(highlightcolor="black")
self.sampleDateLabel.configure(text='''Date''')
self.sampleDateLabel.configure(font=font20)
self.sampleRatioLabel = tk.Label(self.sampleParamsFrame)
self.sampleRatioLabel.place(relx=0.41555, rely=0.5689, height=24, width=170
, bordermode='ignore')
self.sampleRatioLabel.configure(activebackground="#f9f9f9")
self.sampleRatioLabel.configure(activeforeground="black")
self.sampleRatioLabel.configure(background="#d9d9d9")
self.sampleRatioLabel.configure(disabledforeground="#a3a3a3")
self.sampleRatioLabel.configure(foreground="#000000")
self.sampleRatioLabel.configure(highlightbackground="#d9d9d9")
self.sampleRatioLabel.configure(highlightcolor="black")
self.sampleRatioLabel.configure(text='''WBC : RBC''')
self.sampleRatioLabel.configure(font=font20)
self.sampleBloodCountText = tk.Text(self.sampleParamsFrame)
self.sampleBloodCountText.place(relx=0.062, rely=0.752, relheight=0.157
, relwidth=0.335, bordermode='ignore')
self.sampleBloodCountText.configure(background="white")
self.sampleBloodCountText.configure(font=font20)
self.sampleBloodCountText.configure(foreground="black")
self.sampleBloodCountText.configure(highlightbackground="#d9d9d9")
self.sampleBloodCountText.configure(highlightcolor="black")
self.sampleBloodCountText.configure(insertbackground="black")
self.sampleBloodCountText.configure(selectbackground="#c4c4c4")
self.sampleBloodCountText.configure(selectforeground="black")
self.sampleBloodCountText.configure(wrap="word")
self.sampleBloodCountLabel = tk.Label(self.sampleParamsFrame)
self.sampleBloodCountLabel.place(relx=0.43, rely=0.796, height=24, width=100
, bordermode='ignore')
self.sampleBloodCountLabel.configure(activebackground="#f9f9f9")
self.sampleBloodCountLabel.configure(activeforeground="black")
self.sampleBloodCountLabel.configure(background="#d9d9d9")
self.sampleBloodCountLabel.configure(disabledforeground="#a3a3a3")
self.sampleBloodCountLabel.configure(foreground="#000000")
self.sampleBloodCountLabel.configure(highlightbackground="#d9d9d9")
self.sampleBloodCountLabel.configure(highlightcolor="black")
self.sampleBloodCountLabel.configure(text='''Counts''')
self.sampleBloodCountLabel.configure(font=font20)
#configuration for image frame that contains the current image
self.imageFrame = tk.LabelFrame(self.root)
self.imageFrame.place(relx=0.3969, rely=0.006, relheight=0.638
, relwidth=0.600)
self.imageFrame.configure(relief='groove')
self.imageFrame.configure(borderwidth="0")
self.imageFrame.configure(foreground="black")
self.imageFrame.configure(background="#d9d9d9")
self.imageFrame.configure(highlightbackground="#d9d9d9")
self.imageFrame.configure(highlightcolor="black")
self.path = "xi_example.tiff"
self.img = ImageTk.PhotoImage(Image.open(self.path))
self.panel = tk.Label(self.imageFrame,image=self.img)
self.panel.image = self.img
self.panel.pack()
#%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%~~~~~~~~~~BEGIN CALLBACKS~~~~~~~~~~~~%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%#
# 1.) CAMERA #
# 2.) FOCUS #
# 3.) ANALYZE BLOOD #
# 4.) BORDER UPDATE #
# 5.) DATAFILE #
# 6.) AUTO START & E-STOP #
# 7.) LED #
# 8.) CAROUSEL #
# 9.) KEYPAD #
# 10.) RUN #
#%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%#
#~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ CAMERA FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~#
# #
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#opens and closes cam adjust window based on parity of counter
def camAdjust(self):
if(self.camAdjustCnt %2 == 0):
self.system.cam.camAdjust()
else:
self.system.cam.camAdjustExit()
self.camAdjustCnt+=1
#updates Cam Temp Reading every 6 secs
def getCamTemp(self):
x = self.system.cam.getTemp()
x =str(round(x,2))
self.camTempText.delete("1.0","end")
self.camTempText.insert(tk.END,x)
self.root.after(6000,self.getCamTemp)
#shows Image on GUI after capture image is pressed
def showIm(self):
self.updateStatusText("Camera Busy")
self.updateStatusBorder("yellow")
self.system.busy = True
self.system.cam.showIm()
self.system.busy = False
self.system.GUI.updateStatusText()
self.system.GUI.updateStatusBorder()
#prints camera aq status every 6 seconds (useful for debugging)
def camAqStatus(self):
print(self.system.cam.getAqStatus())
self.root.after(6000,self.camAqStatus)
#every 100ms checks if image needs to be updated and updates when necessary
def updateImage(self):
if(self.system.imgChange==True):
# self.newImg = ImageTk.PhotoImage(Image.open(self.path))
self.newImg = ImageTk.PhotoImage(self.system.currImage)
self.panel.configure(image=self.newImg)
self.panel.image = self.newImg
self.panel.pack()
self.system.imgChange = False
self.root.after(100,self.updateImage)
#starts new seperate thread for video calling system.control, which is for controlling threads
#thread runs in a infinite loop, checking parity of vidPowerCnt to determine when to turn on or off
#can be killed by e-stop(thread not killed,but video turned off)
def videoPower(self):
self.system.cam.vidPowerCnt +=1
if(self.system.cam.vidPowerCnt %2 == 1):
self.system.cam.set_bg_black()
self.updateStatusText("Camera Busy")
self.updateStatusBorder("yellow")
self.system.busy = True
if(self.system.videoThreadStarted==False):
self.system.control.combine('cam')
else:
self.system.cam.showIm()
self.system.busy = False
self.system.GUI.updateStatusText()
self.system.GUI.updateStatusBorder()
self.system.cam.showIm()
#~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ FOCUS FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~#
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#runs autofoucs routine in seperate thread, can be killed by e-stop
def autoFocus(self):
self.system.control.combine('focus')
#manual single step down
def jogDwn(self):
self.system.focus.jogDown()
self.focusPosText.delete("1.0", "end")
self.focusPosText.insert(tk.END,self.system.focus.zVar())
#manual single step up
def jogUp(self):
self.system.focus.jogUp()
self.focusPosText.delete("1.0", "end")
self.focusPosText.insert(tk.END,self.system.focus.zVar())
#~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ANALYZE BLOOD FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~#
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#runs blood counting in seperate thread, cannot be killed by e-stop (but could easily be added)
def countBlood(self):
self.updateStatusText("Analyzing Blood")
self.updateStatusBorder("yellow")
self.system.busy = True
analyze = Thread(target = self.system.man.countBlood)
analyze.start()
#sets pathology in utility class from user input into min and max pathology
def setPathology(self):
self.system.util.setPathology(float(self.minPathologyText.get("1.0",tk.END)),float(self.maxPathologyText.get("1.0",tk.END)))
#~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ BORDER UPDATE FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~#
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#added next two functions to remove/add pathology border. root.update()
#forces the GUI to redraw (fixed issue of not remoiving border at new analysis)
#root.update is dangerous and i'm not sure if its causing nested event loops
# should probably switch to root.after similar to camTemp function
def removePathologyBorder(self):
self.sampleBloodCountText.delete("1.0", "end")
self.sampleRatioText.delete("1.0", "end")
self.sampleRatioText.configure(highlightthickness=0)
self.root.update()
#for setting red border on wbc/rbc ratio on GUI, same warning as above
def addPathologyBorder(self,ratio):
if(self.system.util.pathologyWarn(ratio)):
self.sampleRatioText.configure(highlightbackground="red")
self.sampleRatioText.configure(highlightthickness=6)
self.root.update()
#for setting status border on GUI, same warning as above
def updateStatusBorder(self,color="green"):
if(self.system.busy == True):