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A non-guided transcriptome assembly usually aims to the de novo assembly method of creating a transcriptome without the aid of a reference genome. The availability of Next Generation Sequencing (NGS) technologies allows researchers to capture the spatial or temporal profile of gene expresion from a huge types of biological samples. The Following markdown describe an integrative workflow analysis in RNA-seq data based on the current bioinformatic methods.

-Ricardo Gore

Outlines

• Processing Raw libraries .
• Running Trinity .
• Transrating Assembly .
• Annotation .
• Abundance .
• Differential Expression .
• Functional Annotation (In process ...).
• Further analysis (In process ...) -Cluster Profile -ReactomePA

Flow-analysis diagram

Starts working on ssh serve

ssh user@omica

Password: ******

The Center for Scientific Research and Higher Education of Ensenada (CICESE), Mexico have a computer cluster (Paper) with many bioinformatic apps installed within ready to implement by subscribed users.

Please, email PhD A. Lago in order to request a cluster account (a prior authorization from your responsal)

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