From 5b26d9154d521984b62c74a8177daa6c60943058 Mon Sep 17 00:00:00 2001
From: eduardo-proteinms Experimental Overview
different calibrants.
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Tutorial Figure 1. Experimental Overview @@ -144,7 +144,7 @@
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Before exporting your first transition list, first save your document to the AbsoluteQuant folder by doing the following: @@ -242,7 +242,7 @@
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You can also see that all of the other settings are appropriate for this very simple target list. @@ -290,7 +290,7 @@
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What to inspect when looking at the chromatographic traces for the standards: @@ -368,13 +368,13 @@
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Or the heavy precursor:
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Verify the following: @@ -402,7 +402,7 @@
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@@ -420,7 +420,7 @@
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The slope and intercept are displayed on the calibration curve. They can be used to convert between peak area ratio and concentration with a diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-1.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-1.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-1.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-1.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-10.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-10.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-10.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-10.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-11.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-11.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-11.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-11.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-12.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-12.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-12.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-12.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-13.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-13.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-13.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-13.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-14.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-14.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-14.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-14.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-15.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-15.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-15.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-15.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-16.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-16.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-16.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-16.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-2.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-2.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-2.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-2.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-3.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-3.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-3.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-3.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-4.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-4.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-4.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-4.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-5.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-5.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-5.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-5.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-6.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-6.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-6.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-6.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-7.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-7.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-7.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-7.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-8.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-8.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-8.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-8.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-9.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-9.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-9.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-9.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-0.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-experimental-overview.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/image-0.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/en/s-experimental-overview.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/index.html b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/index.html index 8a918a46ab..6268ef75cc 100644 --- a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/index.html +++ b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/index.html @@ -32,7 +32,7 @@
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チュートリアル図1. 実験概要 @@ -112,7 +112,7 @@
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最初のトランジションリストをエクスポートする前に、まず次の手順でドキュメントをAbsoluteQuantフォルダに保存します。 @@ -205,7 +205,7 @@
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また、他の設定もすべてこのターゲットリストに適切であることがわかります。 @@ -250,7 +250,7 @@
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標準のクロマトグラフトレースを見るときに注目すべきは以下の項目です。 @@ -320,13 +320,13 @@
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または安定同位体標識ペプチドのプリカーサーイオンを選択できます。
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以下を確認します。 @@ -351,7 +351,7 @@
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@@ -369,7 +369,7 @@
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校正曲線線に勾配と切片が表示されています。これを標準のy = m * x + b式と共に使用してピーク領域比と濃度を変換できます。 diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/invariant.html b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/invariant.html index e1cb11687a..c63cf91cd3 100644 --- a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/invariant.html +++ b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/invariant.html @@ -60,7 +60,7 @@
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Tutorial Figure 1. Experimental Overview @@ -144,7 +144,7 @@
- +
- +
- +
- +
- +
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Before exporting your first transition list, first save your document to the AbsoluteQuant folder by doing the following: @@ -242,7 +242,7 @@
- +
You can also see that all of the other settings are appropriate for this very simple target list. @@ -290,7 +290,7 @@
- +
- +
- +
What to inspect when looking at the chromatographic traces for the standards: @@ -368,13 +368,13 @@
- +
Or the heavy precursor:
- +
Verify the following: @@ -402,7 +402,7 @@
- +
@@ -420,7 +420,7 @@
- +
- +
- +
The slope and intercept are displayed on the calibration curve. They can be used to convert between peak area ratio and concentration with a diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-1.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-1.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-1.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-1.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-10.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-10.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-10.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-10.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-11.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-11.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-11.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-11.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-12.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-12.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-12.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-12.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-13.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-13.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-13.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-13.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-14.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-14.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-14.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-14.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-15.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-15.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-15.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-15.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-16.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-16.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-16.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-16.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-2.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-2.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-2.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-2.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-3.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-3.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-3.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-3.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-4.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-4.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-4.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-4.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-5.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-5.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-5.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-5.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-6.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-6.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-6.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-6.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-7.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-7.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-7.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-7.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-8.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-8.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-8.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-8.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-9.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-9.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-9.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-9.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-0.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-experimental-overview.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/image-0.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/ja/s-experimental-overview.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/index.html b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/index.html index e81670bc23..6a3b356ff1 100644 --- a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/index.html +++ b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/index.html @@ -35,7 +35,7 @@
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教程图 1. 实验概述 @@ -115,7 +115,7 @@
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在导出您的第一个离子对列表前,先将您的文档保存至 AbsoluteQuant 文件夹,操作如下: @@ -208,7 +208,7 @@
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您还可看到其他所有设置都适合于这个非常简单的目标列表。 @@ -254,7 +254,7 @@
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查看标准的色谱痕量时所要检查的内容: @@ -324,13 +324,13 @@
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或是选择重母离子:
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验证以下项: @@ -354,7 +354,7 @@
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@@ -372,7 +372,7 @@
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斜率和截距显示在校准曲线上。使用标准的 y = m * x + b 方程式可以在峰面积比和浓度之间进行转换 diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/invariant.html b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/invariant.html index dbd479ec9b..5b06d8c431 100644 --- a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/invariant.html +++ b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/invariant.html @@ -60,7 +60,7 @@
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Tutorial Figure 1. Experimental Overview @@ -144,7 +144,7 @@
- +
- +
- +
- +
- +
- +
Before exporting your first transition list, first save your document to the AbsoluteQuant folder by doing the following: @@ -242,7 +242,7 @@
- +
You can also see that all of the other settings are appropriate for this very simple target list. @@ -290,7 +290,7 @@
- +
- +
- +
What to inspect when looking at the chromatographic traces for the standards: @@ -368,13 +368,13 @@
- +
Or the heavy precursor:
- +
Verify the following: @@ -402,7 +402,7 @@
- +
@@ -420,7 +420,7 @@
- +
- +
- +
The slope and intercept are displayed on the calibration curve. They can be used to convert between peak area ratio and concentration with a diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-1.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-1.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-1.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-1.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-10.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-10.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-10.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-10.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-11.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-11.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-11.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-11.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-12.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-12.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-12.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-12.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-13.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-13.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-13.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-13.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-14.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-14.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-14.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-14.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-15.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-15.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-15.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-15.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-16.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-16.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-16.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-16.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-2.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-2.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-2.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-2.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-3.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-3.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-3.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-3.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-4.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-4.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-4.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-4.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-5.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-5.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-5.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-5.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-6.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-6.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-6.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-6.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-7.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-7.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-7.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-7.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-8.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-8.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-8.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-8.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-9.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-9.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-9.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-9.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-0.png b/pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-experimental-overview.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/image-0.png rename to pwiz_tools/Skyline/Documentation/Tutorials/AbsoluteQuant/zh-CHS/s-experimental-overview.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/AuditLog/en/index.html b/pwiz_tools/Skyline/Documentation/Tutorials/AuditLog/en/index.html index e3142f9d7a..d84626b8f7 100644 --- a/pwiz_tools/Skyline/Documentation/Tutorials/AuditLog/en/index.html +++ b/pwiz_tools/Skyline/Documentation/Tutorials/AuditLog/en/index.html @@ -81,7 +81,7 @@
- +
In the top right corner, you can see that audit logging is currently enabled, which is the default. By unchecking the box, audit logging can be @@ -120,7 +120,7 @@
- +
Each dark gray message and all messages below it until the next dark gray message or the end of the audit log represent a single undoable @@ -137,7 +137,7 @@
- +
Before audit logging was introduced into Skyline, both messages here would have only said Changed settings. Therefore, even if @@ -177,7 +177,7 @@
- +
You can see the change you just undid through the Audit Log grid. @@ -207,7 +207,7 @@
- +
- +
If you do not see all of the elements shown above, do the following: @@ -229,7 +229,7 @@
- +
Note that there is a magnifying glass icon next to the new audit log entry. This appears whenever there is extra information associated with an @@ -242,7 +242,7 @@
- +
The extra information window shows all the information that was pasted into the grid when inserting the peptide, including the column header @@ -284,7 +284,7 @@
- +
The audit log did not create copies of the files that were imported, but rather just stored the paths of files (here shortened to just the file @@ -300,7 +300,7 @@
- +
- +
The changes made are listed under the heading Peptide Settings – Quantification changed and each step is captured in a @@ -383,13 +383,13 @@
- +
Review the Audit Log grid to see that it shows the following:
- +
The Undo-Redo messages indicates that 16 values were pasted into the Document Grid. There are a total of 17 detailed messages. @@ -402,7 +402,7 @@
- +
This displays a much more concise view of the data that was pasted into the document and again the data can be easily copied and pasted to @@ -436,7 +436,7 @@
- +
The integration boundaries look acceptable and changing them may simply add variance to the peak area calculations. However, you might feel the @@ -450,7 +450,7 @@
- +
Note that the new log entry describes a change related to an item in the Targets window, it uses a format similar to the one @@ -468,7 +468,7 @@
- +
Note that the reason entered in the dark gray row is shown in all three new rows. This is the overall reason for the change. To give a @@ -486,7 +486,7 @@
- +
You can see that the last three points fall off the line, which indicates that they are below the limit of detection for this peptide. These @@ -505,7 +505,7 @@
- +
You should again add a reason to explain why the replicates were excluded by doing the following: @@ -527,14 +527,14 @@
- +
- +
Now the Audit Log view only displays the Undo Redo messages and not the detail messages, presenting a concise @@ -562,7 +562,7 @@
- +
- +
Below is a list of all of the columns you can display and their meaning: @@ -604,7 +604,7 @@
- +
- +
- +
- +
- + | - + |
- +
- +
Examine the peak area patterns for the rest of the peptides belonging to this protein. Is the quantitative pattern for the peptides from this @@ -561,7 +561,7 @@
- +
- +
- +
- +
Explore the data further manually (including some decoys). @@ -606,7 +606,7 @@
- +
Note that the distribution appears to have two peaks, one around 3 and one around 6. To understand this better, it helps to know that these are @@ -639,7 +639,7 @@
- +
- +
Note: As the spectral library for this analysis was constructed from a side-by-side analysis of the same samples. As such, the @@ -685,7 +685,7 @@
- +
- +
- +
You can already see clusters of points around the expected ratios and above the 5% FDR horizontal line for most of the changing peptides. To @@ -733,7 +733,7 @@
- +
- +
- +
Notice that there are still some gray dots. You can click on any of the dots to cause them to be selected in the Targets view @@ -794,7 +794,7 @@
- +
As with the volcano plot, you can click on the individual bars to select them in the Targets view (so that you can confirm @@ -821,7 +821,7 @@
- +
- +
[figure adapted from Navarro, P. et al. A multicenter study benchmarks software tools for label-free proteome quantification. Nature
@@ -126,7 +126,7 @@ Using the Import Peptide Search wizard
The Import Peptide Search wizard should appear looking like this:
- +
- +
Note: 0.95 is the threshold applied to the PeptideProphet probability computed for every peptide spectrum match in the DDA database search – in @@ -155,13 +155,13 @@
- +
- +
- +
- +
- +
- +
Skyline has worked out the Start and End m/z values for the DIA isolation ranges measured on the @@ -310,7 +310,7 @@
- +
You can click-and-drag a rectangle to zoom in or use the mouse scroll-wheel, and see the margins. If you uncheck and re-check Show
@@ -326,7 +326,7 @@ Using the Import Peptide Search wizard
The Import Peptide Search wizard should now look like this:
- +
- +
- +
Notice that with a Min peptides per protein filter of “1” the 12 proteins found in the FASTA file produce 11 “Mapped” @@ -385,7 +385,7 @@
- +
With 6-cores and above all files will be processed in parallel, and on most laptops with 2-cores the import will process 2 files at a time. @@ -395,7 +395,7 @@
- +
It has already been used to re-score the 10 best peaks Skyline found in each set of chromatograms, using its default score, and re-choose the @@ -423,7 +423,7 @@
- +
- +
- + | - + |
- +
- +
Examine the peak area patterns for the rest of the peptides belonging to this protein. Is the quantitative pattern for the peptides from this @@ -567,7 +567,7 @@
- +
- +
- +
Explore the data further manually (including some decoys). @@ -602,7 +602,7 @@
- +
Note that this distribution covers all 6 runs. To understand better how representative it is of each individual run do the following: @@ -634,7 +634,7 @@
- +
- +
Note: As the spectral library for this analysis was constructed from a side-by-side analysis of the same samples. As such, the @@ -680,7 +680,7 @@
- +
- +
- +
You can already see clusters of points around the expected ratios and above the 5% FDR horizontal line for most of the changing peptides. To @@ -728,7 +728,7 @@
- +
- +
- +
Notice that there are still some gray dots. You can click on any of the dots to cause them to be selected in the Targets view @@ -789,7 +789,7 @@
- +
As with the volcano plot, you can click on the individual bars to select them in the Targets view (so that you can confirm @@ -816,7 +816,7 @@
- +
- +
[図の出典: Navarro, P. et al. A multicenter study benchmarks software tools for label-free proteome quantification.Nature Biotech 34, @@ -112,7 +112,7 @@
- +
注:0.95は、DDAデータベース検索において、すべてのペプチド・スペクトル・マッチ(PSM)に対してPeptideProphetで設定した確率の閾値です。このチュートリアルで用いるデータセットでは、偽陽性率(false discovery rate, @@ -129,7 +129,7 @@
- +
- +
- +
- +
- +
- +
- +
Skylineは、質量分析計で測定されたDIA単離範囲の [ 開始 ] および [ 終了 ] @@ -284,7 +284,7 @@
- +
クリック・アンド・ドラッグして囲んだ長方形を使い部分拡大するか、マウスのスクロールホイールを使用して拡大すると、マージンの位置の詳細を見ることができます。[ マージンを表示 ] チェックボックスをオフにし、再びオンにすると、グラフのタイトルが @@ -299,7 +299,7 @@
- +
- +
- +
[タンパク質毎の最小ペプチド数 ] @@ -349,7 +349,7 @@
- +
6コア以上ではすべてのファイルが並列処理され、2コアのほとんどのラップトップでは、インポートは2ファイルずつ処理されます。 @@ -359,7 +359,7 @@
- +
mProphetモデルでは、それぞれのクロマトグラムセットの中から、Skylineがデフォルトとして使用するスコアに基づき選んだ10個のベストピークを見直し、最も高いmProphetスコアを与える10個のピークを選びます。これらのピークのmProphetスコア(SkylineではDetection
@@ -386,7 +386,7 @@ 試料の注釈
[ 注釈を定義 ] ウィンドウは以下のようになります。
- +
- +
- + | - + |
- +
- +
このタンパク質に属する残りのペプチドのピーク領域パターンを調べて下さい。このタンパク質のペプチドの定量パターンは、予想される差異発現パターン(4:1)と一致していますか? @@ -516,7 +516,7 @@
- +
- +
- +
手作業でさらにデータを調べます(デコイを含む)。 @@ -547,7 +547,7 @@
- +
この分布図は6回のランすべてを網羅していることにご注意ください。この分布図が各ランをどの程度よく表しているかを理解するには、以下の操作を行います。 @@ -575,7 +575,7 @@
- +
- +
注:このチュートリアルのスペクトルライブラリは同じ試料を同時に測定した結果を用いて構築しました。そのため、保持時間予測の精度は非常に高くなっています。異なる装置、異なる時間、あるいは試料が異なる場合は、構築したスペクトルライブラリの保持時間の誤差はもっと大きくなると考えられます。 @@ -612,7 +612,7 @@
- +
- +
- +
表示されたボルケーノプロットでは、変化している点の多くが、予想される倍率変化の周辺に集まっており、また、5% FDRを示す水平線の上にあることがわかります。どの点がどの生物種に属するのかを明確にするため次の操作を行って下さい。 @@ -656,7 +656,7 @@
- +
- +
- +
まだグレーのドットがいくつか残っていることに注意してください。これらのドットのどれかをクリックすると、 [ ターゲット ] @@ -711,7 +711,7 @@
- +
ボルケーノプロット同様、個々の棒をクリックすると [ ターゲット ] 表示で選択されます(グラフ内のさまざまなペプチドがどの生物種からのものかを確認できます)。 @@ -733,7 +733,7 @@
- +
- +
[figure adapted from Navarro, P. et al. A multicenter study benchmarks software tools for label-free proteome quantification. Nature
@@ -124,7 +124,7 @@ Using the Import Peptide Search wizard
The Import Peptide Search wizard should appear looking like this:
- +
Note: 0.95 is the threshold applied to the PeptideProphet probability computed for every peptide spectrum match in the DDA database search – in @@ -142,7 +142,7 @@
- +
- +
- +
- +
- +
- +
- +
Skyline has worked out the Start and End m/z values for the DIA isolation ranges measured on the @@ -308,7 +308,7 @@
- +
You can click-and-drag a rectangle to zoom in or use the mouse scroll-wheel, and see the margins. If you uncheck and re-check Show
@@ -324,7 +324,7 @@ Using the Import Peptide Search wizard
The Import Peptide Search wizard should now look like this:
- +
- +
- +
Notice that with a Min peptides per protein filter of “1” the 13 proteins found in the FASTA file produce 12 @@ -378,7 +378,7 @@
- +
With 6-cores and above all files will be processed in parallel, and on most laptops with 2-cores the import will process 2 files at a time. @@ -388,7 +388,7 @@
- +
It has already been used to re-score the 10 best peaks Skyline found in each set of chromatograms, using its default score, and re-choose the @@ -416,7 +416,7 @@
- +
- +
- + | - + |
- +
- +
Examine the peak area patterns for the rest of the peptides belonging to this protein. Is the quantitative pattern for the peptides from this @@ -559,7 +559,7 @@
- +
- +
- +
Explore the data further manually (including some decoys). @@ -594,7 +594,7 @@
- +
Note that this distribution covers all 6 runs. To understand better how representative it is of each individual run do the following: @@ -626,7 +626,7 @@
- +
- +
Note: As the spectral library for this analysis was constructed from a side-by-side analysis of the same samples. As such, the @@ -672,7 +672,7 @@
- +
- +
- +
You can already see clusters of points around the expected ratios and above the 5% FDR horizontal line for most of the changing peptides. To @@ -720,7 +720,7 @@
- +
- +
- +
Notice that there are still some gray dots. You can click on any of the dots to cause them to be selected in the Targets view @@ -781,7 +781,7 @@
- +
As with the volcano plot, you can click on the individual bars to select them in the Targets view (so that you can confirm @@ -808,7 +808,7 @@
- +
- +
[图片取自 Navarro, P. et al. A multicenter study benchmarks software tools for label-free proteome quantification. Nature Biotech 34, @@ -112,7 +112,7 @@
- +
注意:0.95 是DDA 数据库搜索中每个肽段谱图匹配度计算的 PeptideProphet 概率阈值 - 在这个特定的数据集中,这相当于 0.2% 的 PSM 错误发现率,但此值在不同数据集中会有所不同,因此应在此处输入您要使用的错误发现率的概率阈值。 @@ -128,7 +128,7 @@
- +
- +
- +
- +
- +
- +
- +
Skyline 已解出在质谱仪上测得的 DIA 分离范围的开始和结束质荷比值。可以看出它们重叠了 2 质荷比,为补偿重叠,Skyline 在分离窗口的每侧增加了 1 @@ -280,7 +280,7 @@
- +
您可以单击并拖动矩形进行放大,或是使用鼠标滚轮查看边距。如果取消选中显示边距后又重新选中,图形名称将在测量窗口和提取窗口之间切换,并且粉红色的边距条消失后又会重新出现。图中没有红色的显示间距或黄色的显示单循环重叠,二者均为 @@ -294,7 +294,7 @@
- +
- +
- +
可以发现,每个蛋白质的最少肽段数过滤器设置为 "1" 时,FASTA 文件中的 13 个蛋白质产生的剩余蛋白质为 12 个。这是因为有 1 @@ -343,7 +343,7 @@
- +
在 6 核及以上的处理器中,所有文件将并行处理;在大多数配备双核处理器的笔记本电脑上,导入过程中一次处理 2 个文件。 @@ -352,7 +352,7 @@
- +
系统采用默认的分数对 Skyline 在每组色谱图中找到的 10 个最优峰重新评分,并将这些峰重新选择为 mProphet 得分最高的峰。这些 mProphet 分数(在 Skyline 中叫做检测 Z 得分)进行扩展后,以使 1.0 表示与平均 @@ -377,7 +377,7 @@
- +
- +
- + | - + |
- +
- +
检查属于该蛋白质的其余肽段的峰面积模式。蛋白质肽段的定量模式是否与预期的差异化调控模式 (4:1) 一致? @@ -505,7 +505,7 @@
- +
- +
- +
进一步手动探索数据(包括一些诱饵)。 @@ -536,7 +536,7 @@
- +
请注意,此分布涵盖全部 6 次运行。为进一步理解每次运行的代表性,请执行以下操作: @@ -563,7 +563,7 @@
- +
- +
注意:因为这项分析的谱图库是通过对相同样品进行并排分析构建而成,所以保留时间预测的准确性非常好。在对不同仪器不同时间不同样品分析创建的外部谱图库中,保留时间将导致这些预测中的误差较大。 @@ -602,7 +602,7 @@
- +
- +
- +
对于大多数变化的肽段,已可以看到出现在预期比率附近和 5% FDR 水平线以上的点簇。为了更好地了解哪些点属于哪种生物体,请执行以下操作: @@ -645,7 +645,7 @@
- +
- +
- +
请注意,图中仍有一些灰点。您可以单击其中任意的点,这样它们在目标视图中将处于选中状态,并且将在图中以红色显示测试。您也可以将鼠标光标悬停在任何点上以获取更多信息。通过这种方式,可以看到灰点属于 iRT @@ -700,7 +700,7 @@
- +
与火山图一样,您可以单击目标视图中的单个条形以将其选定(这样便可确认图中的不同肽段来自哪个物种)。 @@ -723,7 +723,7 @@
- +
- +
[figure adapted from Navarro, P. et al. A multicenter study benchmarks software tools for label-free proteome quantification. Nature
@@ -124,7 +124,7 @@ Using the Import Peptide Search wizard
The Import Peptide Search wizard should appear looking like this:
- +
Note: 0.95 is the threshold applied to the PeptideProphet probability computed for every peptide spectrum match in the DDA database search – in @@ -142,7 +142,7 @@
- +
- +
- +
- +
- +
- +
- +
Skyline has worked out the Start and End m/z values for the DIA isolation ranges measured on the @@ -308,7 +308,7 @@
- +
You can click-and-drag a rectangle to zoom in or use the mouse scroll-wheel, and see the margins. If you uncheck and re-check Show
@@ -324,7 +324,7 @@ Using the Import Peptide Search wizard
The Import Peptide Search wizard should now look like this:
- +
- +
- +
Notice that with a Min peptides per protein filter of “1” the 13 proteins found in the FASTA file produce 12 @@ -378,7 +378,7 @@
- +
With 6-cores and above all files will be processed in parallel, and on most laptops with 2-cores the import will process 2 files at a time. @@ -388,7 +388,7 @@
- +
It has already been used to re-score the 10 best peaks Skyline found in each set of chromatograms, using its default score, and re-choose the @@ -416,7 +416,7 @@
- +
- +
- + | - + |
- +
- +
Examine the peak area patterns for the rest of the peptides belonging to this protein. Is the quantitative pattern for the peptides from this @@ -559,7 +559,7 @@
- +
- +
- +
Explore the data further manually (including some decoys). @@ -594,7 +594,7 @@
- +
Note that this distribution covers all 6 runs. To understand better how representative it is of each individual run do the following: @@ -626,7 +626,7 @@
- +
- +
Note: As the spectral library for this analysis was constructed from a side-by-side analysis of the same samples. As such, the @@ -672,7 +672,7 @@
- +
- +
- +
You can already see clusters of points around the expected ratios and above the 5% FDR horizontal line for most of the changing peptides. To @@ -720,7 +720,7 @@
- +
- +
- +
Notice that there are still some gray dots. You can click on any of the dots to cause them to be selected in the Targets view @@ -781,7 +781,7 @@
- +
As with the volcano plot, you can click on the individual bars to select them in the Targets view (so that you can confirm @@ -808,7 +808,7 @@
- +
- +
[figure adapted from Navarro, P. et al. A multicenter study benchmarks software tools for label-free proteome quantification. Nature
@@ -124,7 +124,7 @@ Using the Import Peptide Search wizard
The Import Peptide Search wizard should appear looking like this:
- +
- +
Note: 0.95 is the threshold applied to the PeptideProphet probability computed for every peptide spectrum match in the DDA database search – in @@ -153,13 +153,13 @@
- +
- +
- +
- +
- +
- +
Skyline has worked out the Start and End m/z values for the DIA isolation ranges measured on the @@ -308,7 +308,7 @@
- +
You can click-and-drag a rectangle to zoom in or use the mouse scroll-wheel, and see the margins. If you uncheck and re-check Show
@@ -324,7 +324,7 @@ Using the Import Peptide Search wizard
The Import Peptide Search wizard should now look like this:
- +
- +
- +
Notice that with a Min peptides per protein filter of “1” the 12 proteins found in the FASTA file produce 11 “Mapped” @@ -383,7 +383,7 @@
- +
With 6-cores and above all files will be processed in parallel, and on most laptops with 2-cores the import will process 2 files at a time. @@ -393,7 +393,7 @@
- +
It has already been used to re-score the 10 best peaks Skyline found in each set of chromatograms, using its default score, and re-choose the @@ -421,7 +421,7 @@
- +
- +
- + | - + |
- +
- +
Examine the peak area patterns for the rest of the peptides belonging to this protein. Is the quantitative pattern for the peptides from this @@ -564,7 +564,7 @@
- +
- +
- +
Explore the data further manually (including some decoys). @@ -599,7 +599,7 @@
- +
Note that the distribution appears to have two peaks, one around 0 and one around 5. To understand this better, it helps to know that these are @@ -632,7 +632,7 @@
- +
- +
Note: As the spectral library for this analysis was constructed from a side-by-side analysis of the same samples. As such, the @@ -678,7 +678,7 @@
- +
- +
- +
You can already see clusters of points around the expected ratios and above the 5% FDR horizontal line for most of the changing peptides. To @@ -726,7 +726,7 @@
- +
- +
- +
Notice that there are still some gray dots. You can click on any of the dots to cause them to be selected in the Targets view @@ -787,7 +787,7 @@
- +
As with the volcano plot, you can click on the individual bars to select them in the Targets view (so that you can confirm @@ -814,7 +814,7 @@
- +
- +
[図の出典:Navarro, P. et al. A multicenter study benchmarks software tools for label-free proteome quantification.Nature Biotech 34, @@ -113,7 +113,7 @@
- +
注:0.95は、DDAデータベース検索において、すべてのペプチド・スペクトル・マッチ(PSM)に対してPeptideProphetで設定した確率の閾値です。このチュートリアルで用いるデータセットでは、偽陽性率(false discovery rate, @@ -130,7 +130,7 @@
- +
- +
- +
- +
- +
- +
- +
Skylineは、質量分析計で測定されたDIA単離範囲の [ 開始 ] および [ 終了 ] @@ -285,7 +285,7 @@
- +
クリック・アンド・ドラッグして囲んだ長方形を使い部分拡大するか、マウスのスクロールホイールを使用して拡大すると、マージンの位置の詳細を見ることができます。[ マージンを表示 ] チェックボックスをオフにし、再びオンにすると、グラフのタイトルが @@ -300,7 +300,7 @@
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[ タンパク質毎の最小ペプチド数 ] @@ -350,7 +350,7 @@
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6コア以上ではすべてのファイルが並列処理され、2コアのほとんどのラップトップでは、インポートは2ファイルずつ処理されます。 @@ -360,7 +360,7 @@
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mProphetモデルでは、それぞれのクロマトグラムセットの中から、Skylineがデフォルトとして使用するスコアに基づき選んだ10個のベストピークを見直し、最も高いmProphetスコアを与える10個のピークを選びます。これらのピークのmProphetスコア(SkylineではDetection
@@ -387,7 +387,7 @@ 試料の注釈
[ 注釈を定義 ] ウィンドウは以下のようになります。
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このタンパク質に属する残りのペプチドのピーク領域パターンを調べて下さい。このタンパク質のペプチドの定量パターンは、予想される差異発現パターン(4:1)と一致していますか? @@ -517,7 +517,7 @@
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手作業でさらにデータを調べます(デコイを含む)。 @@ -548,7 +548,7 @@
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分布には、0周辺と5周辺とピークが2つあるように見えることに注意してください。これをよりよく理解するには、これが6回のランすべてでの質量誤差値であることを知っていると有用です。 @@ -577,7 +577,7 @@
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注:このチュートリアルのスペクトルライブラリは同じ試料を同時に測定した結果を用いて構築しました。そのため、保持時間予測の精度は非常に高くなっています。異なる装置、異なる時間、あるいは試料が異なる場合は、構築したスペクトルライブラリの保持時間の誤差はもっと大きくなると考えられます。 @@ -614,7 +614,7 @@
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表示されたボルケーノプロットでは、変化している点の多くが、予想される倍率変化の周辺に集まっており、また、5% FDRを示す水平線の上にあることがわかります。どの点がどの生物種に属するのかを明確にするため次の操作を行って下さい。 @@ -658,7 +658,7 @@
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まだグレーのドットがいくつか残っていることに注意してください。これらのドットのどれかをクリックすると、 [ ターゲット ] @@ -713,7 +713,7 @@
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ボルケーノプロット同様、個々の棒をクリックすると [ ターゲット ] 表示で選択されます(グラフ内のさまざまなペプチドがどの生物種からのものかを確認できます)。 @@ -736,7 +736,7 @@
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[figure adapted from Navarro, P. et al. A multicenter study benchmarks software tools for label-free proteome quantification. Nature
@@ -124,7 +124,7 @@ Using the Import Peptide Search wizard
The Import Peptide Search wizard should appear looking like this:
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Note: 0.95 is the threshold applied to the PeptideProphet probability computed for every peptide spectrum match in the DDA database search – in @@ -142,7 +142,7 @@
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Skyline has worked out the Start and End m/z values for the DIA isolation ranges measured on the @@ -308,7 +308,7 @@
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You can click-and-drag a rectangle to zoom in or use the mouse scroll-wheel, and see the margins. If you uncheck and re-check Show
@@ -324,7 +324,7 @@ Using the Import Peptide Search wizard
The Import Peptide Search wizard should now look like this:
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Notice that with a Min peptides per protein filter of “1” the 13 proteins found in the FASTA file produce 12 @@ -378,7 +378,7 @@
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With 6-cores and above all files will be processed in parallel, and on most laptops with 2-cores the import will process 2 files at a time. @@ -388,7 +388,7 @@
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It has already been used to re-score the 10 best peaks Skyline found in each set of chromatograms, using its default score, and re-choose the @@ -416,7 +416,7 @@
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Examine the peak area patterns for the rest of the peptides belonging to this protein. Is the quantitative pattern for the peptides from this @@ -559,7 +559,7 @@
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Explore the data further manually (including some decoys). @@ -594,7 +594,7 @@
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Note that the distribution appears to have two peaks, one around 0 and one around 5. To understand this better, it helps to know that these are @@ -627,7 +627,7 @@
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Note: As the spectral library for this analysis was constructed from a side-by-side analysis of the same samples. As such, the @@ -673,7 +673,7 @@
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You can already see clusters of points around the expected ratios and above the 5% FDR horizontal line for most of the changing peptides. To @@ -721,7 +721,7 @@
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Notice that there are still some gray dots. You can click on any of the dots to cause them to be selected in the Targets view @@ -782,7 +782,7 @@
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As with the volcano plot, you can click on the individual bars to select them in the Targets view (so that you can confirm @@ -809,7 +809,7 @@
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[图片取自 Navarro, P. et al. A multicenter study benchmarks software tools for label-free proteome quantification. Nature Biotech 34, @@ -110,7 +110,7 @@
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注意:0.95 是 DDA 数据库搜索中每个肽段谱图匹配度计算的 PeptideProphet 概率阈值 - 在这个特定的数据集中,这相当于 0.2% 的 PSM 错误发现率,但此值在不同数据集中会有所不同,因此应在此处输入您要使用的错误发现率的概率阈值。 @@ -126,7 +126,7 @@
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Skyline 已解出在质谱仪上测得的 DIA 分离范围的开始和结束质荷比值。可以看出它们重叠了 1.0 质荷比,为补偿重叠,Skyline 在分离窗口的每侧增加了 0.5 @@ -279,7 +279,7 @@
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您可以单击并拖动矩形进行放大,或是使用鼠标滚轮查看边距。如果取消选中显示边距后又重新选中,图形名称将在测量窗口和提取窗口之间切换,并且粉红色的边距条消失后又会重新出现。图中没有红色的显示间距或黄色的显示单循环重叠,二者均为 @@ -293,7 +293,7 @@
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可以发现,每个蛋白质的最少肽段数过滤器设置为 "1" 时,FASTA 文件中的 13 个蛋白质产生的剩余蛋白质为 12 个。这是因为有 1 @@ -342,7 +342,7 @@
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在 6 核及以上的处理器中,所有文件将并行处理;在大多数配备双核处理器的笔记本电脑上,导入过程中一次处理 2 个文件。 @@ -351,7 +351,7 @@
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系统采用默认的分数对 Skyline 在每组色谱图中找到的 10 个最优峰重新评分,并将这些峰重新选择为 mProphet 得分最高的峰。这些 mProphet 分数(在 Skyline 中叫做检测 Z 得分)进行扩展后,以使 1.0表示与平均 @@ -376,7 +376,7 @@
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检查属于该蛋白质的其余肽段的峰面积模式。该蛋白质的肽段的定量模式是否与预期的差异化调控模式 (4:1) 一致? @@ -504,7 +504,7 @@
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进一步手动探索数据(包括一些诱饵)。 @@ -535,7 +535,7 @@
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请注意,该分布似乎有两个峰,一个在 0 附近,另一个在 5 附近。为更好地理解这一点,应当明白这些是所有 6 次运行的质量误差值。 @@ -562,7 +562,7 @@
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注意:因为这项分析的谱图库是通过对相同样品进行并排分析构建而成,所以保留时间预测的准确性非常好。在对不同仪器不同时间不同样品分析创建的外部谱图库中,保留时间将导致这些预测中的误差较大。 @@ -601,7 +601,7 @@
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对于大多数变化的肽段,已可以看到出现在预期比率附近和 5% FDR 水平线以上的点簇。为了更好地了解哪些点属于哪种生物体,请执行以下操作: @@ -644,7 +644,7 @@
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请注意,图中仍有一些灰点。您可以单击其中任意的点,这样它们在目标视图中将处于选中状态,并且将在图中以红色显示测试。您也可以将鼠标光标悬停在任何点上以获取更多信息。通过这种方式,可以看到灰点属于 iRT @@ -699,7 +699,7 @@
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与火山图一样,您可以单击目标视图中的单个条形以将其选定(这样便可确认图中的不同肽段来自哪个物种)。 @@ -722,7 +722,7 @@
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[figure adapted from Navarro, P. et al. A multicenter study benchmarks software tools for label-free proteome quantification. Nature
@@ -122,7 +122,7 @@ Using the Import Peptide Search wizard
The Import Peptide Search wizard should appear looking like this:
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Note: 0.95 is the threshold applied to the PeptideProphet probability computed for every peptide spectrum match in the DDA database search – in @@ -140,7 +140,7 @@
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Skyline has worked out the Start and End m/z values for the DIA isolation ranges measured on the @@ -306,7 +306,7 @@
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You can click-and-drag a rectangle to zoom in or use the mouse scroll-wheel, and see the margins. If you uncheck and re-check Show
@@ -322,7 +322,7 @@ Using the Import Peptide Search wizard
The Import Peptide Search wizard should now look like this:
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Notice that with a Min peptides per protein filter of “1” the 13 proteins found in the FASTA file produce 12 @@ -376,7 +376,7 @@
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With 6-cores and above all files will be processed in parallel, and on most laptops with 2-cores the import will process 2 files at a time. @@ -386,7 +386,7 @@
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It has already been used to re-score the 10 best peaks Skyline found in each set of chromatograms, using its default score, and re-choose the @@ -414,7 +414,7 @@
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Examine the peak area patterns for the rest of the peptides belonging to this protein. Is the quantitative pattern for the peptides from this @@ -557,7 +557,7 @@
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Explore the data further manually (including some decoys). @@ -592,7 +592,7 @@
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Note that the distribution appears to have two peaks, one around 0 and one around 5. To understand this better, it helps to know that these are @@ -625,7 +625,7 @@
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Note: As the spectral library for this analysis was constructed from a side-by-side analysis of the same samples. As such, the @@ -671,7 +671,7 @@
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You can already see clusters of points around the expected ratios and above the 5% FDR horizontal line for most of the changing peptides. To @@ -719,7 +719,7 @@
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Notice that there are still some gray dots. You can click on any of the dots to cause them to be selected in the Targets view @@ -780,7 +780,7 @@
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As with the volcano plot, you can click on the individual bars to select them in the Targets view (so that you can confirm @@ -807,7 +807,7 @@
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[figure adapted from Navarro, P. et al. A multicenter study benchmarks software tools for label-free proteome quantification. Nature
@@ -119,7 +119,7 @@ Using the Import Peptide Search wizard
The Run Peptide Search wizard should appear looking like this:
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Skyline has worked out the Start and End m/z values for the DIA isolation ranges measured on the @@ -253,7 +253,7 @@
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You can click-and-drag a rectangle to zoom in or use the mouse scroll-wheel, and see the margins. If you uncheck and re-check Show
@@ -269,7 +269,7 @@ Using the Import Peptide Search wizard
The Import Peptide Search wizard should now look like this:
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The main setting here is “Instrument preset” which sets some established defaults for DIA-Umpire parameters according to what kind of @@ -342,7 +342,7 @@
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The Max q-value field sets a false discovery rate (FDR) limit allowed for the peptide matches in the search results. In this @@ -360,7 +360,7 @@
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After the search is finished: @@ -374,13 +374,13 @@
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Notice that with a Min peptides per protein filter of “1” the 13 proteins found in the FASTA file produce 9 @@ -419,14 +419,14 @@
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After the import is completed, Skyline will show you the mProphet model you requested on the Import FASTA page of the Import Peptide Search wizard. It should look like this:
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It has already been used to re-score the 10 best peaks Skyline found in each set of chromatograms, using its default score, and re-choose the @@ -462,10 +462,10 @@
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Examine the peak area patterns for the rest of the peptides belonging to this protein. Is the quantitative pattern for the peptides from this @@ -520,7 +520,7 @@
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Explore the data further manually (including some decoys). @@ -555,7 +555,7 @@
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Note that the distribution appears to have two peaks, one around 0 and one around 5. To understand this better, it helps to know that these are @@ -588,7 +588,7 @@
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Note: The spectral library for this analysis was constructed from the DIA-Umpire pseudo-DDA spectra. As such, the accuracy of diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/image-1.png b/pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/s-1.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/image-1.png rename to pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/s-1.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/image-10.png b/pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/s-10.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/image-10.png rename to pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/s-10.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/image-11.png b/pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/s-11.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/image-11.png rename 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pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/image-14.png rename to pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/s-14.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/image-15.png b/pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/s-15.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/image-15.png rename to pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/s-15.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/image-16.png b/pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/s-16.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/image-16.png rename to pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/s-16.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/image-17.png 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pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/image-9.png rename to pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/s-9.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/image-0.png b/pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/s-ttof-label-free-proteome-quantification.png similarity index 100% rename from pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/image-0.png rename to pwiz_tools/Skyline/Documentation/Tutorials/DIA-Umpire-TTOF/en/s-ttof-label-free-proteome-quantification.png diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/DIA/en/index.html b/pwiz_tools/Skyline/Documentation/Tutorials/DIA/en/index.html index 29dc5a7a03..f6fc8c8f64 100644 --- a/pwiz_tools/Skyline/Documentation/Tutorials/DIA/en/index.html +++ b/pwiz_tools/Skyline/Documentation/Tutorials/DIA/en/index.html @@ -154,7 +154,7 @@
- +
- + | - + |
The plots for this peptide should now look like this:
- +
The combination of the cyan ID lines, the idotp of 0.99, and the dotp of 096 should give you high confidence in this peak assignment. @@ -773,7 +773,7 @@
- +
You can get a sense of scale in intensity (ions per second) by looking at the Peak Areas plots where the precurors (MS1) total @@ -796,7 +796,7 @@
Skyline will show the Full Scan view displaying a range of the MS1 scan from which the precursor chromatogram points were - extracted. You could see the entire MS1 scan by clicking on the button with the magnifying glass and a plus () in + extracted. You could see the entire MS1 scan by clicking on the button with the magnifying glass and a plus () in the upper right corner of the view. Instead, do the following:
- +
The intensities in the centroid-mode peaks are shown as sticks. Intensities that were used to create the points on the chromatograms are @@ -817,7 +817,7 @@
@@ -836,7 +836,7 @@
- +
You can see the MS/MS peak that contributed the point to the chromatogram you just clicked on highlighted in aqua and annotated y10. The fact @@ -849,7 +849,7 @@
- +
Again, you can see the tallest peak in a series highlighted and annotated, with the following peaks in the series 0.5 m/z apart, @@ -871,7 +871,7 @@
@@ -881,19 +881,19 @@
- +
At 41.68 minutes, you can see the extracted peaks shift left in m/z:
- +
And at 41.70 minutes, they have shifted out of the +/- 20 ppm extraction ranges:
- +
Extraction from centroided MS/MS spectra for DIA, especially on Thermo Orbitrap instruments, has proven more reliable in broad statistical
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zcmV-Y0DIAの単離スキームの設定
[ トランジションの設定 ] フォームは以下のようになります。
- +
- + | - + |
このペプチドのプロットは、以下のようになります。
- +
青緑色のIDライン、idotp 0.99、そしてdotp 096の組み合わせにより、このピークの割り当てにはかなりの信頼性があります。 @@ -662,7 +662,7 @@
- +
ピーク面積プロットを見ると、強度の規模(イオン個数/秒)が把握できます。ここでプリカーサー (MS1) は総数100万近く、プロダクト (MS/MS) @@ -682,7 +682,7 @@
- Skylineは、プリカーサークロマトグラムの点が抽出された幅広いMS1スキャンを表示するフルスキャンビューを表示します。虫眼鏡のボタンをクリックし、ビューの右上角にあるプラス () + Skylineは、プリカーサークロマトグラムの点が抽出された幅広いMS1スキャンを表示するフルスキャンビューを表示します。虫眼鏡のボタンをクリックし、ビューの右上角にあるプラス () をクリックするとMS1スキャン全体が見えるようになります。代わりに、以下の操作を行います。
- +
セントロイドモードピークの強度は棒のようになります。クロマトグラフで点を形成していた強度は、クロマトグラムの色で強調表示され、抽出範囲は影付き領域として表示されます。抽出されていなかったM+3およびM+4ピークが影付きピークの右側にあるのが見えます。また、干渉しているピークの頂点を形成していたスペクトルが、このプリカーサーの積分されたピークの左側に表示されています。ターゲットペプチドよりも1ダルトン重い2価のプリカーサーの同位体分布全体が見られます。モノアイソトピックピークは、ターゲットのM+1に非常に近くなっています。
70.56分には、ターゲットモノアイソトピックフィルタ範囲にあるピークを含む最初のMS1スペクトルが青色で強調表示されます。70.67分までは、モノアイソトピックピークが最も高い最初のMS1スペクトルは見えません。 @@ -714,7 +714,7 @@
- +
今クリックしたクロマトグラムの点となったMS/MSピークが水色で強調表示され、y10と注釈が付くことがわかります。同位体ピークは1 m/zずつ離れていることからも、これらが1価のイオンからによるものであるという確信が持てます。 @@ -726,7 +726,7 @@
- +
ここでも、一連の中で一番高いピークが強調表示されて注釈が付きます。そのうち、以下のピークは0.5 @@ -744,7 +744,7 @@
時間が経つほど、M-1(モノアイソトピックプリカーサーの左)にあるピークが強くなっていくのがわかります。 @@ -753,19 +753,19 @@
- +
41.68分で、抽出されたピークのm/zが左にシフトするのがわかります。
- +
そして41.70分では、+/- 20 ppmの抽出範囲を超えてシフトしています。
- +
DIAのセントロイド化されたMS/MSスペクトルからの抽出は、特にThermo diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/DIA/ja/invariant.html b/pwiz_tools/Skyline/Documentation/Tutorials/DIA/ja/invariant.html index 78cae0b16c..fcca7b0594 100644 --- a/pwiz_tools/Skyline/Documentation/Tutorials/DIA/ja/invariant.html +++ b/pwiz_tools/Skyline/Documentation/Tutorials/DIA/ja/invariant.html @@ -154,7 +154,7 @@
- +
- + | - + |
The plots for this peptide should now look like this:
- +
The combination of the cyan ID lines, the idotp of 0.99, and the dotp of 096 should give you high confidence in this peak assignment. @@ -775,7 +775,7 @@
- +
You can get a sense of scale in intensity (ions per second) by looking at the Peak Areas plots where the precurors (MS1) total @@ -798,7 +798,7 @@
Skyline will show the Full Scan view displaying a range of the MS1 scan from which the precursor chromatogram points were - extracted. You could see the entire MS1 scan by clicking on the button with the magnifying glass and a plus () in + extracted. You could see the entire MS1 scan by clicking on the button with the magnifying glass and a plus () in the upper right corner of the view. Instead, do the following:
- +
The intensities in the centroid-mode peaks are shown as sticks. Intensities that were used to create the points on the chromatograms are @@ -819,7 +819,7 @@
@@ -838,7 +838,7 @@
- +
You can see the MS/MS peak that contributed the point to the chromatogram you just clicked on highlighted in aqua and annotated y10. The fact @@ -851,7 +851,7 @@
- +
Again, you can see the tallest peak in a series highlighted and annotated, with the following peaks in the series 0.5 m/z apart, @@ -873,7 +873,7 @@
@@ -883,19 +883,19 @@
- +
At 41.68 minutes, you can see the extracted peaks shift left in m/z:
- +
And at 41.70 minutes, they have shifted out of the +/- 20 ppm extraction ranges:
- +
Extraction from centroided MS/MS spectra for DIA, especially on Thermo Orbitrap instruments, has proven more reliable in broad statistical
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zcmV-Y0为 DIA 设置分离方案
“离子对设置”表单将显示如下:
- +
- + | - + |
这个肽段图现在应如下所示:
- +
在青色 ID 线、0.99 同位素点积和 096 点积的组合之下,您应当对这个峰分配信心十足。 @@ -651,7 +651,7 @@
- +
您可以查看峰面积图来了解强度大小(每秒的离子数),其中母离子 (MS1) 总数接近 100 万,子离子(串联质谱)总数为 20 到 25,000 或 40 到 50 倍的差异。前面看到过,MS1 @@ -670,7 +670,7 @@
- Skyline 将显示全扫描视图,其中显示从中提取母离子色谱图点的 MS1 扫描范围。您可以单击带有放大镜的按钮和视图右上角的加号 () 来查看整个 MS1 扫描。执行以下操作: + Skyline 将显示全扫描视图,其中显示从中提取母离子色谱图点的 MS1 扫描范围。您可以单击带有放大镜的按钮和视图右上角的加号 () 来查看整个 MS1 扫描。执行以下操作:
- +
质心模式峰的强度显示为棒状。用于在色谱图上创建点的强度以色谱图颜色突出显示,提取范围显示为阴影区域。您可以看到未提取到阴影峰右侧的 M+3 和 M+4 峰。您所查看的产生干扰峰顶点的谱图在这个母离子积分峰的左边。您可以看到比目标肽段重 1 道尔顿的双电荷母离子的完整同位素分布。它的单同位素峰非常接近目标的 M+1。
在 70.56 分钟处,应看到第一个包含目标单同位素过滤范围内峰的 MS1 谱图,该谱图突出显示为蓝色。直到 70.67 分钟处,您才会看到第一个包含最强单同位素峰的 MS1 谱图。 @@ -702,7 +702,7 @@
- +
可以看到,对您刚刚在色谱图上单击的点做出贡献的串联质谱峰突出显示为浅绿色,并标注有 y10。从同位素峰相距 1 质荷比这一点,可以更加确信它们是由单电荷离子产生的。 @@ -714,7 +714,7 @@
- +
同样,可以看到对一系列峰中最高的峰进行了突出显示和注释,该系列峰中的后续峰相距 0.5 质荷比,表明是双电荷离子的同位素分布。您应当可以更加确信在子离子色谱图中提取的信号与您的目标相匹配。 @@ -732,7 +732,7 @@
您会看到 M-1(单同位素母离子的左侧)处的峰随时间推移而增长。 @@ -741,19 +741,19 @@
- +
在 41.68 分钟处,可以看到所提取峰的质荷比左移:
- +
在 41.70 分钟处,它们已移出 +/- 20 ppm 的提取范围:
- +
从 DIA 的质心串联质谱谱图中提取,尤其是在 Thermo Orbitrap 仪器上进行,已被证明在广泛的统计测试中更可靠,但在考虑数据时,这仍然是一个有用的概念。当您通常预期它会导致信号增加时,此设置中的干扰会降低总信号。 diff --git a/pwiz_tools/Skyline/Documentation/Tutorials/DIA/zh-CHS/invariant.html b/pwiz_tools/Skyline/Documentation/Tutorials/DIA/zh-CHS/invariant.html index f602bcdd79..e80c115869 100644 --- a/pwiz_tools/Skyline/Documentation/Tutorials/DIA/zh-CHS/invariant.html +++ b/pwiz_tools/Skyline/Documentation/Tutorials/DIA/zh-CHS/invariant.html @@ -154,7 +154,7 @@
- +