diff --git a/api/v1/prefectserver_types.go b/api/v1/prefectserver_types.go
index 60b2225..da3c6e2 100644
--- a/api/v1/prefectserver_types.go
+++ b/api/v1/prefectserver_types.go
@@ -66,6 +66,9 @@ type PrefectServerSpec struct {
 
 	// MigrationJobLabels defines additional labels to add to the migration Job
 	MigrationJobLabels map[string]string `json:"migrationJobLabels,omitempty"`
+
+	// NodeSelector defines the node selector for the Prefect Server Deployment and migration Job
+	NodeSelector map[string]string `json:"nodeSelector,omitempty"`
 }
 
 type EphemeralConfiguration struct {
diff --git a/api/v1/zz_generated.deepcopy.go b/api/v1/zz_generated.deepcopy.go
index c450cd2..db79d76 100644
--- a/api/v1/zz_generated.deepcopy.go
+++ b/api/v1/zz_generated.deepcopy.go
@@ -296,6 +296,13 @@ func (in *PrefectServerSpec) DeepCopyInto(out *PrefectServerSpec) {
 			(*out)[key] = val
 		}
 	}
+	if in.NodeSelector != nil {
+		in, out := &in.NodeSelector, &out.NodeSelector
+		*out = make(map[string]string, len(*in))
+		for key, val := range *in {
+			(*out)[key] = val
+		}
+	}
 }
 
 // DeepCopy is an autogenerated deepcopy function, copying the receiver, creating a new PrefectServerSpec.
diff --git a/deploy/charts/prefect-operator/crds/prefect.io_prefectservers.yaml b/deploy/charts/prefect-operator/crds/prefect.io_prefectservers.yaml
index b19c249..c277467 100644
--- a/deploy/charts/prefect-operator/crds/prefect.io_prefectservers.yaml
+++ b/deploy/charts/prefect-operator/crds/prefect.io_prefectservers.yaml
@@ -1482,6 +1482,12 @@ spec:
                 description: MigrationJobLabels defines additional labels to add to
                   the migration Job
                 type: object
+              nodeSelector:
+                additionalProperties:
+                  type: string
+                description: NodeSelector defines the node selector for the Prefect
+                  Server Deployment and migration Job
+                type: object
               postgres:
                 description: |-
                   Postgres defines whether the server will be deployed with a PostgreSQL backend connecting to the
diff --git a/internal/controller/prefectserver_controller.go b/internal/controller/prefectserver_controller.go
index 2b30357..a601dab 100644
--- a/internal/controller/prefectserver_controller.go
+++ b/internal/controller/prefectserver_controller.go
@@ -339,6 +339,7 @@ func (r *PrefectServerReconciler) ephemeralDeploymentSpec(server *prefectiov1.Pr
 				Labels: server.ServerLabels(),
 			},
 			Spec: corev1.PodSpec{
+				NodeSelector: server.Spec.NodeSelector,
 				Volumes: []corev1.Volume{
 					{
 						Name: "prefect-data",
@@ -419,6 +420,7 @@ func (r *PrefectServerReconciler) sqliteDeploymentSpec(server *prefectiov1.Prefe
 				Labels: server.ServerLabels(),
 			},
 			Spec: corev1.PodSpec{
+				NodeSelector: server.Spec.NodeSelector,
 				Volumes: []corev1.Volume{
 					{
 						Name: "prefect-data",
@@ -486,6 +488,7 @@ func (r *PrefectServerReconciler) postgresDeploymentSpec(server *prefectiov1.Pre
 				Labels: server.ServerLabels(),
 			},
 			Spec: corev1.PodSpec{
+				NodeSelector: server.Spec.NodeSelector,
 				InitContainers: []corev1.Container{
 					r.initContainerWaitForPostgres(server),
 				},
@@ -534,6 +537,7 @@ func (r *PrefectServerReconciler) postgresMigrationJob(server *prefectiov1.Prefe
 				Labels: server.MigrationJobLabels(),
 			},
 			Spec: corev1.PodSpec{
+				NodeSelector: server.Spec.NodeSelector,
 				InitContainers: []corev1.Container{
 					r.initContainerWaitForPostgres(server),
 				},
diff --git a/internal/controller/prefectserver_controller_test.go b/internal/controller/prefectserver_controller_test.go
index 4b2610e..95eb4ed 100644
--- a/internal/controller/prefectserver_controller_test.go
+++ b/internal/controller/prefectserver_controller_test.go
@@ -190,6 +190,9 @@ var _ = Describe("PrefectServer controller", func() {
 							"some":    "additional-label",
 							"another": "extra-label",
 						},
+						NodeSelector: map[string]string{
+							"some": "node-selector",
+						},
 					},
 				}
 				Expect(k8sClient.Create(ctx, prefectserver)).To(Succeed())
@@ -377,6 +380,10 @@ var _ = Describe("PrefectServer controller", func() {
 						FailureThreshold:    2,
 					}))
 				})
+
+				It("should have the correct nodeSelector value", func() {
+					Expect(deployment.Spec.Template.Spec.NodeSelector).To(HaveKeyWithValue("some", "node-selector"))
+				})
 			})
 
 			Describe("the Service", func() {
@@ -1192,6 +1199,9 @@ var _ = Describe("PrefectServer controller", func() {
 							"some":    "additional-label-for-migrations",
 							"another": "extra-label-for-migrations",
 						},
+						NodeSelector: map[string]string{
+							"some": "node-selector",
+						},
 					},
 				}
 				Expect(k8sClient.Create(ctx, prefectserver)).To(Succeed())
@@ -1331,6 +1341,10 @@ var _ = Describe("PrefectServer controller", func() {
 						{Name: "PREFECT_API_DATABASE_MIGRATE_ON_START", Value: "False"},
 					}))
 				})
+
+				It("should have the correct nodeSelector value", func() {
+					Expect(migrateJob.Spec.Template.Spec.NodeSelector).To(HaveKeyWithValue("some", "node-selector"))
+				})
 			})
 		})