diff --git a/pkgs/applications/networking/sniffers/wireshark/default.nix b/pkgs/applications/networking/sniffers/wireshark/default.nix
index 49bf77a483c2d..94bbb480663f5 100644
--- a/pkgs/applications/networking/sniffers/wireshark/default.nix
+++ b/pkgs/applications/networking/sniffers/wireshark/default.nix
@@ -39,14 +39,14 @@
 , makeWrapper
 , wrapGAppsHook
 , withQt ? true
-, qt5 ? null
+, qt6 ? null
 , ApplicationServices
 , SystemConfiguration
 , gmp
 , asciidoctor
 }:
 
-assert withQt -> qt5 != null;
+assert withQt -> qt6 != null;
 
 let
   version = "4.0.5";
@@ -70,6 +70,7 @@ stdenv.mkDerivation {
     # Fix `extcap` and `plugins` paths. See https://bugs.wireshark.org/bugzilla/show_bug.cgi?id=16444
     "-DCMAKE_INSTALL_LIBDIR=lib"
     "-DLEMON_C_COMPILER=cc"
+    "-DUSE_qt6=ON"
   ] ++ lib.optionals (stdenv.buildPlatform != stdenv.hostPlatform) [
     "-DHAVE_C99_VSNPRINTF_EXITCODE=0"
     "-DHAVE_C99_VSNPRINTF_EXITCODE__TRYRUN_OUTPUT="
@@ -79,7 +80,7 @@ stdenv.mkDerivation {
   env.NIX_CFLAGS_COMPILE = toString [ "-DQT_NO_DEBUG" ];
 
   nativeBuildInputs = [ asciidoctor bison cmake ninja flex makeWrapper pkg-config python3 perl ]
-    ++ lib.optionals withQt [ qt5.wrapQtAppsHook wrapGAppsHook ];
+    ++ lib.optionals withQt [ qt6.wrapQtAppsHook wrapGAppsHook ];
 
   depsBuildBuild = [ buildPackages.stdenv.cc ];
 
@@ -108,11 +109,10 @@ stdenv.mkDerivation {
     c-ares
     glib
     zlib
-  ] ++ lib.optionals withQt (with qt5; [ qtbase qtmultimedia qtsvg qttools ])
-  ++ lib.optionals (withQt && stdenv.isLinux) [ qt5.qtwayland ]
+  ] ++ lib.optionals withQt (with qt6; [ qtbase qtmultimedia qtsvg qttools qt5compat ])
+  ++ lib.optionals (withQt && stdenv.isLinux) [ qt6.qtwayland ]
   ++ lib.optionals stdenv.isLinux [ libcap libnl sbc ]
-  ++ lib.optionals stdenv.isDarwin [ SystemConfiguration ApplicationServices gmp ]
-  ++ lib.optionals (withQt && stdenv.isDarwin) (with qt5; [ qtmacextras ]);
+  ++ lib.optionals stdenv.isDarwin [ SystemConfiguration ApplicationServices gmp ];
 
   strictDeps = true;