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env_options.txt
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#Parameter default match [if experimental, what's affected] # comments
PRODUCTION TRUE FALSE # default: refuse experimental settings
CN NKI [A-Za-z0-9_.-]{2,10} # Company name; readgroups and output name
BRCA_CLASS b1.191 (b1\.371|b1|b2) # see README.md
TYPE breast ovarian
OUTCLS cls [A-Za-z0-9_.-]{2,10} # output subdirectory for classification
TAG -GRCh38-blacklist-merged -[A-Za-z0-9._-]+ #postfix for NKI_1m file
# keep mem below your system's or this may crash, `free -h' for what's available. At least 1G is required.
MEM 2G [1-9][0-9]*G
PARALLEL 2 [1-9][0-9]{0,3} # parallel * threads should be < CPU(s)
THREADS 4 [1-9][0-9]{0,3} # ..but mind other users, same for MEM
DEBUG FALSE TRUE # print executed commands
# The filename can differ, if checksum and fai match, the sequence and order of contigs is ok.
FASTA Homo_sapiens.GRCh38.dna.primary_assembly.fa [A-Za-z0-9_.-]{1,64}
#--[ EXPERIMENTAL options below, setting to non-default require PRODUCTION=FALSE ]--#
SETM2C FALSE TRUE classifier # see README.md
CPFCOR TRUE FALSE classifier # crossplatform correction
PL Illumina [A-Za-z0-9_.-]{3,10} classifier # supported platform; @RG
BLACKLIST GRCh38-blacklist-merged.bed [A-Za-z0-9_.-]{1,256}\.bed classifier # blacklisted regions
KBIN_SIZE 20 [1-9][0-9]{0,3} mappability
SEQLEN 65 [1-9][0-9]{0,3} mappability
MINQUAL 15 [0-9]{1,2} mappability
# if any of these change all bets are off..
CHROMINFO /app/chromInfo.txt /input/chromInfo.txt pipeline
BINS /app/windows-20k.txt /input/windows-20k.txt pipeline
GCCONT /app/gccontent-20k.txt /input/gccontent-20k.txt pipeline
CHECKSUM 257604babc88d1a24bffa13f41c39172681c000a1dd396b32ebdcde0d1fa78f6 [a-f0-9]{64} pipeline