diff --git a/reusable-workflow-repo b/reusable-workflow-repo index e24998d..c4c1c85 160000 --- a/reusable-workflow-repo +++ b/reusable-workflow-repo @@ -1 +1 @@ -Subproject commit e24998da8b63cab46f4422abd12571a8ede8b040 +Subproject commit c4c1c8516d686f25a3d8d91004d897f86b1b7346 diff --git a/tutorial_1-bismark.ipynb b/tutorial_1-bismark.ipynb index ddb2509..b5965d8 100644 --- a/tutorial_1-bismark.ipynb +++ b/tutorial_1-bismark.ipynb @@ -59,7 +59,7 @@ "! ~/miniconda3/bin/conda init bash\n", "! ~/miniconda3/bin/conda init zsh\n", "``` \n", - "It would take less time to install some of the tools in this tutorial if you create a new conda environment. But it is a little bit tricky to use a different conda environment within Jupyter in GCP. You can find instructions of how to create a new conda environment [here](https://github.com/STRIDES/NIHCloudLabGCP/docs/How_to_use_conda_envs_as_kernels.ipynb).\n", + "It would take less time to install some of the tools in this tutorial if you create a new conda environment. But it is a little bit tricky to use a different conda environment within Jupyter in GCP. You can find instructions of how to create a new conda environment [here](https://github.com/STRIDES/NIHCloudLabGCP/blob/main/docs/create_conda_env.md).\n", "" ] }, @@ -364,7 +364,7 @@ "\n", "All four DNA strands that arise through bisulfite treatment and subsequent PCR amplification can be sequenced with the same frequency in non-directional libraries, while for directional libraries, adapters are attached to the DNA fragments such that only the original top or bottom strands will be sequenced. In the Bismark alignment step, `--directional` is set to default, so only report OT and OB strands.\n", "\n", - "**Output alignment**. The output .bam files are the binary version of the SAM (Sequence Alignment/Map) format. Please see [here](https://github.com/FelixKrueger/Bismark/tree/master/Docs#bismark-bamsam-output-default) for more detailed explanation of the output .bam/.sam format, where the `XM-tag` is the methylation call string to indicate the methylated status of each C(tyosine) in different contexts: CG (or CpG), CHG or CHH (where H correspond to A, T or C). The methylation call string contains a dot ‘.’ for every position in the BS-read not involving a cytosine,\n", + "**Output alignment**. The output .bam files are the binary version of the SAM (Sequence Alignment/Map) format. Please see [here](https://github.com/FelixKrueger/Bismark/blob/master/docs/bismark/alignment.md#bismark-bamsam-output-default) for more detailed explanation of the output .bam/.sam format, where the `XM-tag` is the methylation call string to indicate the methylated status of each C(tyosine) in different contexts: CG (or CpG), CHG or CHH (where H correspond to A, T or C). The methylation call string contains a dot ‘.’ for every position in the BS-read not involving a cytosine,\n", "or contains one of the following letters (z,Z,x,X,h,H) for the three different cytosine methylation contexts (UPPER CASE = METHYLATED, lower case = unmethylated).\n", "> \n", "\n", @@ -818,7 +818,11 @@ ] } ], - "metadata": {}, + "metadata": { + "language_info": { + "name": "python" + } + }, "nbformat": 4, "nbformat_minor": 5 }