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Got some negative values of MSGFScore in the output file when using ".mgf" file #132

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Sweetsour-crap opened this issue Nov 25, 2021 · 0 comments
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@Sweetsour-crap
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I happened to get some negative values of MSGFScore in the output file when running MS-GF+ with ".mgf" file, I don't know the meaning of these negative values or they are just some bugs of the output.
图片

The complete output file is:
mgf_output.txt
I changed it into .txt file for upload.

Useful extras

  • Command line command used to run MS-GF+:
    java -Xmx35000M -jar MSGFPlus.jar -d database_file -t 20ppm -e 1 -m 3 -ntt 2 -inst 1 -thread 9 -tda 1 -ti -1,2 -n 1 -decoy DECOY -maxLength 50 -maxMissedCleavages 2 -mod mod_file -s mgf_file -o output_mzid_file

java -Xmx35000M -cp MSGFPlus.jar edu.ucsd.msjava.ui.MzIDToTsv -showQValue 1 -showDecoy 1 -unroll 0 -i output_mzid_file

  • Output of 'java --version':
    openjdk version "1.8.0_252"
    OpenJDK Runtime Environment (build 1.8.0_252-b09)
    OpenJDK 64-Bit Server VM (build 25.252-b09, mixed mode)
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